MD01G1091200.v1.1

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0004908
Physical Location & Seq
Forward (+)
20623954 .. 20626907
2954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1091200.v1.1.491

Sequence Viewer

Length: 732 bp
ATGAGAGAAAGGGTGTTAGCTTGTTTTACATTGTTGGGTTACTCCCTGACACTTCAAGCTTTTGAAGTCCAAAAATCGATGGCTTCTGAAGATCAAAGTGAAGAAGCTAAAGCAGCTATTCAAGAACACAAAAAAGCTCCTAAGTTAAATGAGAGGATTCTTTCTTCTCTCTCAAGAAAATCAGTTGCTGCACATCCTTGGCATGATCTTGAAATTGGACCTAAAGCTCCCCATATTTTCAACGTTGTTGTTGAGATCTCAAAGGGAAGCAAAGTCAAATATGAACTTGACAAGAAGACAGGACTCATTAAGGTTGATCGGATTTTGTATTCCTCAGTTGTCTATCCTCACAACTACGGTTTCATCCCTCGCACATTGTGTGAAGACAATGATCCCCTGGATGTGTTGGTTCTCATGCAGGAACCTGTTCATCCGGGCTGTTTTCTGCGAGCCAAAGCGATTGGAGTAATGCCTATGATTGACCAGGGAGAGGAAGATGATAAAATCATTGCAGTCTGTGCCGATGATCCAGCATATAACCATTACACTGACATCAAAGAGCTTCCTCCTCATCGCCTCACTGAGATTCGTCGGTTCTTTGAAGACTACAAGAAGAATGAGAACAAAGAGGTTGCAGTTGACCAGTTTTTGCCTGCTCCCAATGCTGCTACAGTTATCCAGTATTCCATGGACCTTTATGCAGAGTACATAATGCTCACCTTAAGGCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Pyrophosphatase

Protein Analysis

244

Amino Acids

27.75

Weight (kDa)

5.93

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 82 - 233 2.6e-59 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 243
AclWI GGATC 2 cut(s) 386, 521
AcuI CTGAAG 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 378
AfaI GTAC 1 cut(s) 707
AfiI CCNNNNNNNGG 1 cut(s) 490
AflII CTTAAG 1 cut(s) 721
AgsI TTSAA 6 cut(s) 56, 65, 122, 212, 241, 602
AjnI CCWGG 2 cut(s) 396, 483
AluBI AGCT 7 cut(s) 20, 59, 107, 116, 137, 227, 562
AluI AGCT 7 cut(s) 20, 59, 107, 116, 137, 227, 562
AlwI GGATC 2 cut(s) 386, 521
AlwNI CAGNNNCTG 1 cut(s) 188
ApeKI GCWGC 3 cut(s) 113, 188, 665
Asp700I GAANNNNTTC 1 cut(s) 426
AspS9I GGNCC 2 cut(s) 218, 691
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 1 cut(s) 709
AvaII GGWCC 2 cut(s) 218, 691
BbsI GAAGAC 3 cut(s) 302, 390, 609
BbvI GCAGC 3 cut(s) 125, 175, 652
BccI CCATC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 398, 485
BcnI CCSGG 1 cut(s) 435
BfmI CTRYAG 1 cut(s) 669
BfrI CTTAAG 1 cut(s) 721
BglII AGATCT 1 cut(s) 255
BisI GCNGC 3 cut(s) 114, 189, 666
BlsI GCNGC 3 cut(s) 115, 190, 667
Bme1390I CCNGG 3 cut(s) 398, 435, 485
Bme18I GGWCC 2 cut(s) 218, 691
BmgT120I GGNCC 2 cut(s) 218, 691
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 3 cut(s) 398, 435, 485
BpiI GAAGAC 3 cut(s) 302, 390, 609
BpuEI CTTGAG 1 cut(s) 157
BpuMI CCSGG 1 cut(s) 435
Bsa29I ATCGAT 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 197, 396, 484, 687
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse1I ACTGG 2 cut(s) 643, 679
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 2 cut(s) 398, 485
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 197, 396, 484, 687
BseGI GGATG 4 cut(s) 193, 363, 406, 430
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 2 cut(s) 348, 573
BseNI ACTGG 2 cut(s) 643, 679
BseRI GAGGAG 1 cut(s) 558
BseXI GCAGC 3 cut(s) 125, 175, 652
BsgI GTGCAG 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 77
BsiSI CCGG 1 cut(s) 434
BslI CCNNNNNNNGG 1 cut(s) 490
Bsp143I GATC 6 cut(s) 91, 205, 255, 316, 391, 526
Bsp19I CCATGG 1 cut(s) 687
BspCNI CTCAG 2 cut(s) 347, 574
BspDI ATCGAT 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 2 cut(s) 386, 521
BspTI CTTAAG 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 643, 679
BssECI CCNNGG 4 cut(s) 197, 396, 484, 687
BssMI GATC 6 cut(s) 91, 205, 255, 316, 391, 526
BssT1I CCWWGG 2 cut(s) 197, 687
Bst2UI CCWGG 2 cut(s) 398, 485
Bst4CI ACNGT 2 cut(s) 359, 673
BstAFI CTTAAG 1 cut(s) 721
BstAPI GCANNNNNTGC 1 cut(s) 518
BstC8I GCNNGC 2 cut(s) 450, 654
BstDEI CTNAG 3 cut(s) 141, 334, 582
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 4 cut(s) 193, 363, 406, 430
BstKTI GATC 6 cut(s) 94, 208, 258, 319, 394, 529
BstMBI GATC 6 cut(s) 91, 205, 255, 316, 391, 526
BstMWI GCNNNNNNNGC 3 cut(s) 113, 518, 662
BstNI CCWGG 2 cut(s) 398, 485
BstSCI CCNGG 3 cut(s) 396, 433, 483
BstSFI CTRYAG 1 cut(s) 669
BstV1I GCAGC 3 cut(s) 125, 175, 652
BstV2I GAAGAC 3 cut(s) 302, 390, 609
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
Bsu15I ATCGAT 1 cut(s) 77
BsuTUI ATCGAT 1 cut(s) 77
BtgI CCRYGG 1 cut(s) 687
BtgZI GCGATG 1 cut(s) 557
BtsCI GGATG 4 cut(s) 193, 363, 406, 430
BtsIMutI CAGTG 2 cut(s) 546, 579
Cac8I GCNNGC 2 cut(s) 450, 654
CaiI CAGNNNCTG 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 218, 691
ClaI ATCGAT 1 cut(s) 77
Csp6I GTAC 1 cut(s) 706
CviAII CATG 3 cut(s) 203, 415, 688
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 3 cut(s) 141, 334, 582
DpnI GATC 6 cut(s) 93, 207, 257, 318, 393, 528
DpnII GATC 6 cut(s) 91, 205, 255, 316, 391, 526
DraIII CACNNNGTG 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 197, 687
Eco47I GGWCC 2 cut(s) 218, 691
Eco57I CTGAAG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 396, 483
EcoT14I CCWWGG 2 cut(s) 197, 687
ErhI CCWWGG 2 cut(s) 197, 687
FaeI CATG 3 cut(s) 206, 418, 691
FatI CATG 3 cut(s) 202, 414, 687
Fnu4HI GCNGC 3 cut(s) 114, 189, 666
FokI GGATG 4 cut(s) 180, 350, 413, 417
Fsp4HI GCNGC 3 cut(s) 114, 189, 666
GluI GCNGC 3 cut(s) 114, 189, 666
HapII CCGG 1 cut(s) 434
Hin1II CATG 3 cut(s) 206, 418, 691
HincII GTYRAC 1 cut(s) 640
HindII GTYRAC 1 cut(s) 640
HindIII AAGCTT 1 cut(s) 57
HinfI GANTC 3 cut(s) 157, 303, 586
HpaII CCGG 1 cut(s) 434
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 2 cut(s) 88, 321
Hpy188III TCNNGA 3 cut(s) 122, 174, 209
Hpy8I GTNNAC 1 cut(s) 640
Hpy99I CGWCG 1 cut(s) 594
HpyCH4III ACNGT 2 cut(s) 359, 673
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 191, 418, 512, 635, 701
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 518, 662
HpyF3I CTNAG 3 cut(s) 141, 334, 582
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 3 cut(s) 206, 418, 691
Kzo9I GATC 6 cut(s) 91, 205, 255, 316, 391, 526
LmnI GCTCC 3 cut(s) 142, 232, 661
Lsp1109I GCAGC 3 cut(s) 125, 175, 652
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 38
MalI GATC 6 cut(s) 93, 207, 257, 318, 393, 528
MboI GATC 6 cut(s) 91, 205, 255, 316, 391, 526
MboII GAAGA 8 cut(s) 101, 113, 156, 307, 395, 506, 614, 625
MflI RGATCY 1 cut(s) 255
MluCI AATT 1 cut(s) 213
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 9 cut(s) 147, 343, 357, 378, 484, 576, 579, 587, 622
MroXI GAANNNNTTC 1 cut(s) 426
MseI TTAA 3 cut(s) 146, 309, 722
MspCI CTTAAG 1 cut(s) 721
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 3 cut(s) 398, 435, 485
MvaI CCWGG 2 cut(s) 398, 485
MwoI GCNNNNNNNGC 3 cut(s) 113, 518, 662
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 687
NdeII GATC 6 cut(s) 91, 205, 255, 316, 391, 526
NlaIII CATG 3 cut(s) 206, 418, 691
NlaIV GGNNCC 1 cut(s) 423
PdmI GAANNNNTTC 1 cut(s) 426
PfeI GAWTC 2 cut(s) 157, 586
PkrI GCNGC 3 cut(s) 115, 190, 667
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp1406I AACGTT 1 cut(s) 243
Psp6I CCWGG 2 cut(s) 396, 483
PspGI CCWGG 2 cut(s) 396, 483
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 218, 691
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 255
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
SaqAI TTAA 3 cut(s) 146, 309, 722
SatI GCNGC 3 cut(s) 114, 189, 666
Sau3AI GATC 6 cut(s) 91, 205, 255, 316, 391, 526
Sau96I GGNCC 2 cut(s) 218, 691
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 398, 435, 485
SfcI CTRYAG 1 cut(s) 669
SinI GGWCC 2 cut(s) 218, 691
SmlI CTYRAG 2 cut(s) 172, 721
SmoI CTYRAG 2 cut(s) 172, 721
Sse9I AATT 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 396, 433, 483
StyI CCWWGG 2 cut(s) 197, 687
TaaI ACNGT 2 cut(s) 359, 673
TaiI ACGT 1 cut(s) 246
TaqI TCGA 1 cut(s) 77
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 705
TfiI GAWTC 2 cut(s) 157, 586
Tru1I TTAA 3 cut(s) 146, 309, 722
Tru9I TTAA 3 cut(s) 146, 309, 722
TscAI CASTG 2 cut(s) 553, 586
TseI GCWGC 3 cut(s) 113, 188, 665
TspDTI ATGAA 3 cut(s) 297, 352, 419
TspRI CASTG 2 cut(s) 553, 586
Vha464I CTTAAG 1 cut(s) 721
VpaK11BI GGWCC 2 cut(s) 218, 691
XmnI GAANNNNTTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.