Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004908

Physical Location & Seq

Genomic Coordinates Forward (+)

23701145 .. 23703589

Gene Structure 2445 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1161500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGATTCTGTATTTTATGTCTGAAGGAAAGGAAGAAGAAACTAAAACCCAAAAAGCTCCCAAATTGAATGAGAGGATTCTTTCATCTCTGTCAAGGAGATCAGTTGCTGCACATCCTTGGCATGATCTTGAAATTGGACCTAGTGCACCCAATGTTTTCAACGTTGTTATTGAGATAACAAAGGGAAGTAAAGTCAAATATGAACTTGACAAGAAAACAGGACTGATTAAGGTTGATAGGATTTTATATTCGTCGGTGGTGTACCCTCACAATTACGGTTTCATCCCTCGCACCCTGTGCGAAGACAATGATCCACTGGATGTTTTAGTTCTCATGCAGGAACCTGTACTTCCGGGTTGCTTTCTGCGAGCCCGAGCCATTGGAGTAATGCCCATGATTGATCAGGGAGAGAAAGATGATAAGATCATCGCAGTCTGCGCAGATGATCCAGAGTACAGACATTTCACTGAGCTCAATGACCTACCCCCTCATCGCCTTTCTGAGATTCGTCGCTTCTTTGAAGACTACAAGAAGAATGAGAACAAAGAGGTTGCAGTAAACGCCTTTTTGCCCGTTAGCACTGCTCTTGAAGCTATCCAGTACTCAATGGATCTTTATGCTGAGTACATACTCCACACCTTAAGGCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004427 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008150 GO:0010876 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0019915 GO:0033036 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051235 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Pyrophosphatase

Protein Analysis

217

Amino Acids

24.86

Weight (kDa)

5.73

Isoelectric Point (pI)

49.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pyrophosphatase	PF00719	55 - 206	2.5e-59	Inorganic pyrophosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01050 AT1G01050 AT2G46860 AT4G01480 AT4G01480
fragaria_vesca	FvH4_7g17871 FvH4_7g17880 FvH4_7g17880 FvH4_7g17880
malus_domestica	MD01G1091200.v1.1 MD01G1091400.v1.1 MD07G1161500.v1.1
prunus_persica	Prupe.2G200700_v2.0.a1 Prupe.2G200700_v2.0.a1 Prupe.2G200800_v2.0.a1 Prupe.2G200800_v2.0.a1
pyrus_communis	pycom01g11550
rosa_chinensis	RchiOBHm_Chr1g0361351
rosa_laevigata	RLG00000027723 RLG00000027724
rosa_multiflora	Rmu_sc0002119.1_g000027
rosa_roxburghii	Rroxscaffold_4G00295030
rosa_rugosa	Rorug01G0288300 Rorug01G0288400 Rorug01G0288500 Rorug01G0288800
rosa_samantha	Rh1AG299600 Rh1BG263400
rosa_wichuraiana	Rw1G026520 Rw1G026530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	439
AciI	CCGC	1 cut(s)	646
AclI	AACGTT	1 cut(s)	162
AclWI	GGATC	3 cut(s)	305, 440, 618
AcuI	CTGAAG	1 cut(s)	42
AdeI	CACNNNGTG	1 cut(s)	297
AfaI	GTAC	5 cut(s)	263, 348, 455, 602, 626
AfiI	CCNNNNNNNGG	1 cut(s)	645
AflII	CTTAAG	1 cut(s)	640
AgsI	TTSAA	5 cut(s)	67, 131, 160, 521, 590
AluBI	AGCT	3 cut(s)	56, 472, 593
AluI	AGCT	3 cut(s)	56, 472, 593
Alw21I	GWGCWC	2 cut(s)	148, 474
Alw44I	GTGCAC	1 cut(s)	144
AlwI	GGATC	3 cut(s)	305, 440, 618
AlwNI	CAGNNNCTG	1 cut(s)	107
Ama87I	CYCGRG	1 cut(s)	372
ApaLI	GTGCAC	1 cut(s)	144
ApeKI	GCWGC	1 cut(s)	107
AspLEI	GCGC	1 cut(s)	440
AspS9I	GGNCC	1 cut(s)	137
AsuC2I	CCSGG	1 cut(s)	354
AvaI	CYCGRG	1 cut(s)	372
AvaII	GGWCC	1 cut(s)	137
BaeGI	GKGCMC	1 cut(s)	148
BanII	GRGCYC	2 cut(s)	373, 474
BbsI	GAAGAC	2 cut(s)	309, 528
Bbv12I	GWGCWC	2 cut(s)	148, 474
BbvI	GCAGC	1 cut(s)	94
BclI	TGATCA	1 cut(s)	400
BcnI	CCSGG	1 cut(s)	354
BfaI	CTAG	1 cut(s)	141
BfrI	CTTAAG	1 cut(s)	640
BisI	GCNGC	1 cut(s)	108
BlsI	GCNGC	1 cut(s)	109
BmcAI	AGTACT	1 cut(s)	602
Bme1390I	CCNGG	1 cut(s)	354
Bme18I	GGWCC	1 cut(s)	137
BmeT110I	CYCGRG	1 cut(s)	372
BmgT120I	GGNCC	1 cut(s)	137
BmiI	GGNNCC	1 cut(s)	342
BmrFI	CCNGG	1 cut(s)	354
BpiI	GAAGAC	2 cut(s)	309, 528
BpuMI	CCSGG	1 cut(s)	354
BsaJI	CCNNGG	1 cut(s)	116
Bsc4I	CCNNNNNNNGG	1 cut(s)	645
Bse1I	ACTGG	2 cut(s)	321, 598
BseDI	CCNNGG	1 cut(s)	116
BseGI	GGATG	3 cut(s)	112, 282, 325
BseLI	CCNNNNNNNGG	1 cut(s)	645
BseMII	CTCAG	3 cut(s)	459, 492, 612
BseNI	ACTGG	2 cut(s)	321, 598
BseSI	GKGCMC	1 cut(s)	148
BseXI	GCAGC	1 cut(s)	94
BsgI	GTGCAG	1 cut(s)	93
BsiHKAI	GWGCWC	2 cut(s)	148, 474
BsiHKCI	CYCGRG	1 cut(s)	372
BsiSI	CCGG	1 cut(s)	353
BslI	CCNNNNNNNGG	1 cut(s)	645
BsoBI	CYCGRG	1 cut(s)	372
Bsp1286I	GDGCHC	3 cut(s)	148, 373, 474
Bsp143I	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
BspACI	CCGC	1 cut(s)	646
BspCNI	CTCAG	3 cut(s)	460, 493, 613
BspLI	GGNNCC	1 cut(s)	342
BspPI	GGATC	3 cut(s)	305, 440, 618
BspTI	CTTAAG	1 cut(s)	640
BsrI	ACTGG	2 cut(s)	321, 598
BssECI	CCNNGG	1 cut(s)	116
BssMI	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
BssT1I	CCWWGG	1 cut(s)	116
Bst4CI	ACNGT	1 cut(s)	278
BstAFI	CTTAAG	1 cut(s)	640
BstAPI	GCANNNNNTGC	1 cut(s)	297
BstC8I	GCNNGC	1 cut(s)	369
BstDEI	CTNAG	3 cut(s)	468, 501, 621
BstF5I	GGATG	3 cut(s)	112, 282, 325
BstHHI	GCGC	1 cut(s)	440
BstKTI	GATC	7 cut(s)	101, 127, 313, 403, 426, 448, 613
BstMBI	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
BstMWI	GCNNNNNNNGC	4 cut(s)	297, 437, 560, 590
BstSCI	CCNGG	1 cut(s)	352
BstSLI	GKGCMC	1 cut(s)	148
BstV1I	GCAGC	1 cut(s)	94
BstV2I	GAAGAC	2 cut(s)	309, 528
BstX2I	RGATCY	1 cut(s)	610
BstYI	RGATCY	1 cut(s)	610
BtgZI	GCGATG	2 cut(s)	412, 476
BtsCI	GGATG	3 cut(s)	112, 282, 325
BtsI	GCAGTG	1 cut(s)	579
BtsIMutI	CAGTG	3 cut(s)	314, 465, 579
Cac8I	GCNNGC	1 cut(s)	369
CaiI	CAGNNNCTG	1 cut(s)	107
CfoI	GCGC	1 cut(s)	440
Cfr13I	GGNCC	1 cut(s)	137
Csp6I	GTAC	5 cut(s)	262, 347, 454, 601, 625
CviAII	CATG	3 cut(s)	122, 334, 394
CviJI	RGCY	5 cut(s)	56, 371, 377, 472, 593
CviKI_1	RGCY	5 cut(s)	56, 371, 377, 472, 593
CviQI	GTAC	5 cut(s)	262, 347, 454, 601, 625
DdeI	CTNAG	3 cut(s)	468, 501, 621
DpnI	GATC	7 cut(s)	100, 126, 312, 402, 425, 447, 612
DpnII	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
DraIII	CACNNNGTG	1 cut(s)	297
Ecl136II	GAGCTC	1 cut(s)	472
Eco130I	CCWWGG	1 cut(s)	116
Eco24I	GRGCYC	2 cut(s)	373, 474
Eco47I	GGWCC	1 cut(s)	137
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	1 cut(s)	42
Eco88I	CYCGRG	1 cut(s)	372
EcoICRI	GAGCTC	1 cut(s)	472
EcoT14I	CCWWGG	1 cut(s)	116
EcoT38I	GRGCYC	2 cut(s)	373, 474
ErhI	CCWWGG	1 cut(s)	116
FaeI	CATG	3 cut(s)	125, 337, 397
FaiI	YATR	8 cut(s)	17, 123, 201, 247, 335, 395, 618, 629
FatI	CATG	3 cut(s)	121, 333, 393
FbaI	TGATCA	1 cut(s)	400
Fnu4HI	GCNGC	1 cut(s)	108
FokI	GGATG	3 cut(s)	99, 269, 332
FriOI	GRGCYC	2 cut(s)	373, 474
Fsp4HI	GCNGC	1 cut(s)	108
FspBI	CTAG	1 cut(s)	141
FspI	TGCGCA	1 cut(s)	439
GlaI	GCGC	1 cut(s)	439
GluI	GCNGC	1 cut(s)	108
HapII	CCGG	1 cut(s)	353
HhaI	GCGC	1 cut(s)	440
Hin1II	CATG	3 cut(s)	125, 337, 397
Hin6I	GCGC	1 cut(s)	438
HinP1I	GCGC	1 cut(s)	438
HinfI	GANTC	3 cut(s)	4, 76, 505
HpaII	CCGG	1 cut(s)	353
Hpy166II	GTNNAC	3 cut(s)	146, 262, 559
Hpy188I	TCNGA	2 cut(s)	22, 502
Hpy188III	TCNNGA	3 cut(s)	128, 449, 587
Hpy8I	GTNNAC	3 cut(s)	146, 262, 559
Hpy99I	CGWCG	2 cut(s)	256, 513
HpyAV	CCTTC	1 cut(s)	17
HpyCH4III	ACNGT	1 cut(s)	278
HpyCH4IV	ACGT	1 cut(s)	162
HpyCH4V	TGCA	4 cut(s)	110, 146, 337, 554
HpyF10VI	GCNNNNNNNGC	4 cut(s)	297, 437, 560, 590
HpyF3I	CTNAG	3 cut(s)	468, 501, 621
HpySE526I	ACGT	1 cut(s)	162
Hsp92II	CATG	3 cut(s)	125, 337, 397
HspAI	GCGC	1 cut(s)	438
Ksp22I	TGATCA	1 cut(s)	400
Kzo9I	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
LmnI	GCTCC	1 cut(s)	61
LpnPI	CCDG	9 cut(s)	204, 302, 308, 323, 357, 366, 389, 462, 611
Lsp1109I	GCAGC	1 cut(s)	94
MaeI	CTAG	1 cut(s)	141
MaeII	ACGT	1 cut(s)	162
MalI	GATC	7 cut(s)	100, 126, 312, 402, 425, 447, 612
MboI	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
MboII	GAAGA	5 cut(s)	44, 47, 314, 533, 544
MflI	RGATCY	1 cut(s)	610
MhlI	GDGCHC	3 cut(s)	148, 373, 474
MluCI	AATT	3 cut(s)	62, 132, 271
MnlI	CCTC	5 cut(s)	66, 276, 297, 498, 541
MseI	TTAA	2 cut(s)	228, 641
MspCI	CTTAAG	1 cut(s)	640
MspI	CCGG	1 cut(s)	353
MspR9I	CCNGG	1 cut(s)	354
MwoI	GCNNNNNNNGC	4 cut(s)	297, 437, 560, 590
NciI	CCSGG	1 cut(s)	354
NdeII	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
NlaIII	CATG	3 cut(s)	125, 337, 397
NlaIV	GGNNCC	1 cut(s)	342
NsbI	TGCGCA	1 cut(s)	439
PfeI	GAWTC	3 cut(s)	4, 76, 505
PkrI	GCNGC	1 cut(s)	109
Psp124BI	GAGCTC	1 cut(s)	474
Psp1406I	AACGTT	1 cut(s)	162
PspN4I	GGNNCC	1 cut(s)	342
PspPI	GGNCC	1 cut(s)	137
PstNI	CAGNNNCTG	1 cut(s)	107
PsuI	RGATCY	1 cut(s)	610
RsaI	GTAC	5 cut(s)	263, 348, 455, 602, 626
RsaNI	GTAC	5 cut(s)	262, 347, 454, 601, 625
SacI	GAGCTC	1 cut(s)	474
SaqAI	TTAA	2 cut(s)	228, 641
SatI	GCNGC	1 cut(s)	108
Sau3AI	GATC	7 cut(s)	98, 124, 310, 400, 423, 445, 610
Sau96I	GGNCC	1 cut(s)	137
ScaI	AGTACT	1 cut(s)	602
ScrFI	CCNGG	1 cut(s)	354
SduI	GDGCHC	3 cut(s)	148, 373, 474
SinI	GGWCC	1 cut(s)	137
SmlI	CTYRAG	1 cut(s)	640
SmoI	CTYRAG	1 cut(s)	640
Sse9I	AATT	3 cut(s)	62, 132, 271
SsiI	CCGC	1 cut(s)	646
SspMI	CTAG	1 cut(s)	141
SstI	GAGCTC	1 cut(s)	474
StyD4I	CCNGG	1 cut(s)	352
StyI	CCWWGG	1 cut(s)	116
TaaI	ACNGT	1 cut(s)	278
TaiI	ACGT	1 cut(s)	165
TasI	AATT	3 cut(s)	62, 132, 271
TatI	WGTACW	4 cut(s)	346, 453, 600, 624
TfiI	GAWTC	3 cut(s)	4, 76, 505
Tru1I	TTAA	2 cut(s)	228, 641
Tru9I	TTAA	2 cut(s)	228, 641
TscAI	CASTG	3 cut(s)	321, 472, 586
TseI	GCWGC	1 cut(s)	107
TspDTI	ATGAA	3 cut(s)	72, 216, 271
TspRI	CASTG	3 cut(s)	321, 472, 586
Vha464I	CTTAAG	1 cut(s)	640
VneI	GTGCAC	1 cut(s)	144
VpaK11BI	GGWCC	1 cut(s)	137
XspI	CTAG	1 cut(s)	141
ZrmI	AGTACT	1 cut(s)	602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.