Rh1AG299600

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004908
Physical Location & Seq
Forward (+)
53112289 .. 53132948
20660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG299600.1

Sequence Viewer

Length: 618 bp
ATGACTGAAGAAAAAAAACCAACTCCGAAATTAAATGAGAGGATTCTTTCATCTTTGTCAAGGAAATCAGTTGCTGCACATCCTTGGCATGATCTTGAAATTGGACCATCAGCTCCCCAAATTTTCAATGTTGTTGTTGAGATATCACAGGGAAGCAAAGTCAAATATGAACTTGACAAGAAAACAGGATTAATTAAGGTTGATCGGATTTTGTACTCGTCAGTGGTCTATCCTCATAACTATGGTTTCATCCCTCGCACCTTGTGTGAAGACAATGATCCACTGGATGTCTTAGTTCTCATGCAGGAACCTGTTATTCCGGGTGCATTTCTGCGTGCCAAAGCCATTGGACTAATGCCCATGATTGACCAGGGAGAGAAAGATGATAAGATCATTGCAGTCTGTGCTGACGATCCAGAGTATACGCATTATACTGAACTCAACGACCTACCCCCTCACCGCCTTTCTGAAATCCGTCGCTTCTTTGAAGACTACAAGAAAAATGAGAACAAAGAGGTTGCGGTTAACGCCTTTTTGCCTGCCACATCCGCTCTTGAAGCTATCCAGTACTCCATGGATCTTTATGCTGAGTACATACTGCACACCTTAAGGCGGTAA

Protein Analysis

205

Amino Acids

23.41

Weight (kDa)

5.55

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyrophosphatase PF00719 44 - 195 2.4e-58 Inorganic pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 551
AccI GTMKAC 1 cut(s) 422
AciI CCGC 4 cut(s) 460, 521, 549, 613
AclWI GGATC 3 cut(s) 272, 407, 585
AcsI RAATTY 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 27
AdeI CACNNNGTG 1 cut(s) 264
AfaI GTAC 3 cut(s) 215, 569, 593
AfiI CCNNNNNNNGG 1 cut(s) 612
AflII CTTAAG 1 cut(s) 607
AgsI TTSAA 4 cut(s) 98, 127, 488, 557
AjnI CCWGG 1 cut(s) 369
AluBI AGCT 2 cut(s) 113, 560
AluI AGCT 2 cut(s) 113, 560
AlwI GGATC 3 cut(s) 272, 407, 585
AlwNI CAGNNNCTG 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 120
AseI ATTAAT 1 cut(s) 191
AspS9I GGNCC 1 cut(s) 104
AsuC2I CCSGG 1 cut(s) 321
AsuHPI GGTGA 1 cut(s) 449
AvaII GGWCC 1 cut(s) 104
BbsI GAAGAC 2 cut(s) 276, 495
BbvI GCAGC 1 cut(s) 61
BccI CCATC 1 cut(s) 115
BciT130I CCWGG 1 cut(s) 371
BcnI CCSGG 1 cut(s) 321
BfrI CTTAAG 1 cut(s) 607
BisI GCNGC 1 cut(s) 75
BlsI GCNGC 1 cut(s) 76
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 2 cut(s) 321, 371
Bme18I GGWCC 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 104
BmiI GGNNCC 1 cut(s) 309
BmrFI CCNGG 2 cut(s) 321, 371
BpiI GAAGAC 2 cut(s) 276, 495
BpuMI CCSGG 1 cut(s) 321
BsaJI CCNNGG 3 cut(s) 83, 370, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 612
Bse1I ACTGG 2 cut(s) 288, 565
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 3 cut(s) 83, 370, 573
BseGI GGATG 4 cut(s) 79, 249, 292, 545
BseLI CCNNNNNNNGG 1 cut(s) 612
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 1 cut(s) 579
BseNI ACTGG 2 cut(s) 288, 565
BseXI GCAGC 1 cut(s) 61
BsgI GTGCAG 2 cut(s) 60, 584
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 612
Bsp143I GATC 6 cut(s) 91, 202, 277, 390, 412, 577
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 4 cut(s) 460, 521, 549, 613
BspCNI CTCAG 1 cut(s) 580
BspLI GGNNCC 1 cut(s) 309
BspPI GGATC 3 cut(s) 272, 407, 585
BspTI CTTAAG 1 cut(s) 607
BsrBI CCGCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 393
BsrI ACTGG 2 cut(s) 288, 565
BssECI CCNNGG 3 cut(s) 83, 370, 573
BssMI GATC 6 cut(s) 91, 202, 277, 390, 412, 577
BssNAI GTATAC 1 cut(s) 423
BssT1I CCWWGG 2 cut(s) 83, 573
Bst1107I GTATAC 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 371
BstAFI CTTAAG 1 cut(s) 607
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 2 cut(s) 336, 540
BstDEI CTNAG 2 cut(s) 292, 588
BstDSI CCRYGG 1 cut(s) 573
BstF5I GGATG 4 cut(s) 79, 249, 292, 545
BstKTI GATC 6 cut(s) 94, 205, 280, 393, 415, 580
BstMBI GATC 6 cut(s) 91, 202, 277, 390, 412, 577
BstMWI GCNNNNNNNGC 4 cut(s) 404, 527, 548, 557
BstNI CCWGG 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 319, 369
BstV1I GCAGC 1 cut(s) 61
BstV2I GAAGAC 2 cut(s) 276, 495
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BstZ17I GTATAC 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 4 cut(s) 79, 249, 292, 545
BtsIMutI CAGTG 2 cut(s) 228, 281
Cac8I GCNNGC 2 cut(s) 336, 540
CaiI CAGNNNCTG 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 104
Csp6I GTAC 3 cut(s) 214, 568, 592
CviAII CATG 4 cut(s) 89, 301, 361, 574
CviJI RGCY 3 cut(s) 113, 344, 560
CviKI_1 RGCY 3 cut(s) 113, 344, 560
CviQI GTAC 3 cut(s) 214, 568, 592
DdeI CTNAG 2 cut(s) 292, 588
DpnI GATC 6 cut(s) 93, 204, 279, 392, 414, 579
DpnII GATC 6 cut(s) 91, 202, 277, 390, 412, 577
DraIII CACNNNGTG 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 83, 573
Eco32I GATATC 1 cut(s) 144
Eco47I GGWCC 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 83, 573
ErhI CCWWGG 2 cut(s) 83, 573
FaeI CATG 4 cut(s) 92, 304, 364, 577
FatI CATG 4 cut(s) 88, 300, 360, 573
FblI GTMKAC 1 cut(s) 422
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 4 cut(s) 66, 236, 299, 532
Fsp4HI GCNGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 75
HapII CCGG 1 cut(s) 320
Hin1II CATG 4 cut(s) 92, 304, 364, 577
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 1 cut(s) 43
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 1 cut(s) 320
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 2 cut(s) 423, 526
Hpy188I TCNGA 3 cut(s) 27, 207, 469
Hpy188III TCNNGA 3 cut(s) 95, 416, 554
Hpy8I GTNNAC 2 cut(s) 423, 526
Hpy99I CGWCG 1 cut(s) 480
HpyCH4V TGCA 5 cut(s) 77, 304, 326, 398, 601
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 527, 548, 557
HpyF3I CTNAG 2 cut(s) 292, 588
Hsp92II CATG 4 cut(s) 92, 304, 364, 577
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 6 cut(s) 91, 202, 277, 390, 412, 577
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 1 cut(s) 61
MalI GATC 6 cut(s) 93, 204, 279, 392, 414, 579
MbiI CCGCTC 1 cut(s) 551
MboI GATC 6 cut(s) 91, 202, 277, 390, 412, 577
MboII GAAGA 3 cut(s) 20, 281, 500
MflI RGATCY 1 cut(s) 577
MluCI AATT 4 cut(s) 29, 99, 120, 192
MnlI CCTC 5 cut(s) 33, 243, 264, 465, 508
MseI TTAA 5 cut(s) 32, 191, 195, 525, 608
MslI CAYNNNNRTG 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 607
MspI CCGG 1 cut(s) 320
MspR9I CCNGG 2 cut(s) 321, 371
MvaI CCWGG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 404, 527, 548, 557
NciI CCSGG 1 cut(s) 321
NcoI CCATGG 1 cut(s) 573
NdeII GATC 6 cut(s) 91, 202, 277, 390, 412, 577
NlaIII CATG 4 cut(s) 92, 304, 364, 577
NlaIV GGNNCC 1 cut(s) 309
PacI TTAATTAA 1 cut(s) 195
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 309
PspPI GGNCC 1 cut(s) 104
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 1 cut(s) 577
RsaI GTAC 3 cut(s) 215, 569, 593
RsaNI GTAC 3 cut(s) 214, 568, 592
RseI CAYNNNNRTG 1 cut(s) 240
SaqAI TTAA 5 cut(s) 32, 191, 195, 525, 608
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 6 cut(s) 91, 202, 277, 390, 412, 577
Sau96I GGNCC 1 cut(s) 104
ScaI AGTACT 1 cut(s) 569
ScrFI CCNGG 2 cut(s) 321, 371
SetI ASST 8 cut(s) 115, 201, 263, 313, 450, 519, 562, 608
SinI GGWCC 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 240
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 4 cut(s) 29, 99, 120, 192
SsiI CCGC 4 cut(s) 460, 521, 549, 613
StyD4I CCNGG 2 cut(s) 319, 369
StyI CCWWGG 2 cut(s) 83, 573
TasI AATT 4 cut(s) 29, 99, 120, 192
TatI WGTACW 3 cut(s) 213, 567, 591
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 5 cut(s) 32, 191, 195, 525, 608
Tru9I TTAA 5 cut(s) 32, 191, 195, 525, 608
TscAI CASTG 2 cut(s) 228, 288
TseI GCWGC 1 cut(s) 74
TspDTI ATGAA 3 cut(s) 39, 183, 238
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 2 cut(s) 228, 288
Vha464I CTTAAG 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 104
VspI ATTAAT 1 cut(s) 191
XapI RAATTY 1 cut(s) 120
XmiI GTMKAC 1 cut(s) 422
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.