Rorug01G0288800

Soluble inorganic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004908
Physical Location & Seq
Forward (+)
40378772 .. 40379269
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0288800.1

Sequence Viewer

Length: 498 bp
ATGGCACATACTAATCTTGGTGCTATGGCTACTCGCAATCCAACCGACTCTGCAAGGGTGACTTTGGGAGCGGCGGGTACGACAGCTATGCAGGACAACCATTGGTATATGGTTGGGCGTCTGCTCGGGCCAAAAACTCGCTTTCCTGGTTTCAAAGGGACCATTCATTCGATTTGGCGGATCAAGTCGGGGCTTTCTGTCCATGATGCTGGAGATCGATTTATTTTTCAGTTTGATCATGAGGCTAATCGAAATAGGGCGCTACATGGAGGACCGTGGTTTCACAGAAGTACGATGTTGCTGGTCGGTGACTATGATGCGGTTGGCTCGGTGGAGGCAGTCCCTTTGTGGAGTATGGAGATCTGGGTGGTGGTTAAAGGGCTACCGGTGGCTCTTCTTAACAAGGCAGCCCTAAATCTGATAGGGTCTGTTGTGGGTCAAGTCATCCTGTTTGATCAAACCGCGCTTCGGCGGAAGGAAGAGAAGCAGAGAATCTGA

Protein Analysis

165

Amino Acids

18.27

Weight (kDa)

10.07

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 30 - 104 1.7e-08 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 71
AccII CGCG 1 cut(s) 464
AciI CCGC 6 cut(s) 71, 74, 178, 320, 462, 472
AclWI GGATC 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 118
AfaI GTAC 2 cut(s) 79, 292
AfiI CCNNNNNNNGG 1 cut(s) 468
AgeI ACCGGT 1 cut(s) 385
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
AlwI GGATC 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 151, 183
AsiGI ACCGGT 1 cut(s) 385
AspLEI GCGC 2 cut(s) 262, 466
AspS9I GGNCC 3 cut(s) 128, 159, 272
AsuHPI GGTGA 2 cut(s) 70, 320
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 2 cut(s) 159, 272
BbvI GCAGC 1 cut(s) 419
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 2 cut(s) 235, 454
BfoI RGCGCY 1 cut(s) 263
BglII AGATCT 1 cut(s) 360
BisI GCNGC 2 cut(s) 72, 408
BlsI GCNGC 2 cut(s) 73, 409
Bme1390I CCNGG 1 cut(s) 147
Bme18I GGWCC 2 cut(s) 159, 272
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 3 cut(s) 128, 159, 272
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 2 cut(s) 196, 307
BpmI CTGGAG 1 cut(s) 231
Bsa29I ATCGAT 1 cut(s) 217
BsaHI GRCGYC 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 275
BsaWI WCCGGW 1 cut(s) 385
Bsc4I CCNNNNNNNGG 1 cut(s) 468
Bse118I RCCGGY 1 cut(s) 385
BseBI CCWGG 1 cut(s) 147
BseCI ATCGAT 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 275
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 1 cut(s) 468
BseXI GCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 1 cut(s) 130
BshTI ACCGGT 1 cut(s) 385
BshVI ATCGAT 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 1 cut(s) 386
BslFI GGGAC 2 cut(s) 172, 326
BslI CCNNNNNNNGG 1 cut(s) 468
BsmFI GGGAC 2 cut(s) 172, 326
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 125
Bsp143I GATC 5 cut(s) 180, 214, 235, 360, 454
BspACI CCGC 6 cut(s) 71, 74, 178, 320, 462, 472
BspANI GGCC 1 cut(s) 130
BspDI ATCGAT 1 cut(s) 217
BspFNI CGCG 1 cut(s) 464
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 399
BsrBI CCGCTC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 385
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 275
BssMI GATC 5 cut(s) 180, 214, 235, 360, 454
BssNI GRCGYC 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 2 cut(s) 399, 474
BstACI GRCGYC 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 1 cut(s) 444
BstFNI CGCG 1 cut(s) 464
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 2 cut(s) 262, 466
BstKTI GATC 5 cut(s) 183, 217, 238, 363, 457
BstMBI GATC 5 cut(s) 180, 214, 235, 360, 454
BstNI CCWGG 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 145
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 360
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 1 cut(s) 360
Bsu15I ATCGAT 1 cut(s) 217
BsuRI GGCC 1 cut(s) 130
BsuTUI ATCGAT 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 1 cut(s) 444
CciI TCATGA 1 cut(s) 238
CfoI GCGC 2 cut(s) 262, 466
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 128, 159, 272
ClaI ATCGAT 1 cut(s) 217
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 78, 291
CspAI ACCGGT 1 cut(s) 385
CviAII CATG 3 cut(s) 203, 239, 266
CviJI RGCY 9 cut(s) 29, 86, 130, 193, 245, 327, 382, 392, 410
CviKI_1 RGCY 9 cut(s) 29, 86, 130, 193, 245, 327, 382, 392, 410
CviQI GTAC 2 cut(s) 78, 291
DpnI GATC 5 cut(s) 182, 216, 237, 362, 456
DpnII GATC 5 cut(s) 180, 214, 235, 360, 454
Eam1104I CTCTTC 2 cut(s) 399, 474
EarI CTCTTC 2 cut(s) 399, 474
EciI GGCGGA 2 cut(s) 193, 487
Eco47I GGWCC 2 cut(s) 159, 272
Eco88I CYCGRG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 206, 242, 269
FalI AAGNNNNNCTT 2 cut(s) 46, 78
FaqI GGGAC 2 cut(s) 172, 326
FatI CATG 3 cut(s) 202, 238, 265
FauI CCCGC 1 cut(s) 67
FbaI TGATCA 2 cut(s) 235, 454
Fnu4HI GCNGC 2 cut(s) 72, 408
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 72, 408
GlaI GCGC 2 cut(s) 261, 465
GluI GCNGC 2 cut(s) 72, 408
GsuI CTGGAG 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 386
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 262, 466
Hin1I GRCGYC 1 cut(s) 118
Hin1II CATG 3 cut(s) 206, 242, 269
Hin6I GCGC 2 cut(s) 260, 464
HinP1I GCGC 2 cut(s) 260, 464
HinfI GANTC 2 cut(s) 47, 492
HpaII CCGG 1 cut(s) 386
HphI GGTGA 2 cut(s) 70, 320
Hpy188I TCNGA 2 cut(s) 420, 497
Hpy188III TCNNGA 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 53, 91
Hsp92I GRCGYC 1 cut(s) 118
Hsp92II CATG 3 cut(s) 206, 242, 269
HspAI GCGC 2 cut(s) 260, 464
Ksp22I TGATCA 2 cut(s) 235, 454
Kzo9I GATC 5 cut(s) 180, 214, 235, 360, 454
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 8 cut(s) 77, 132, 159, 195, 287, 349, 399, 461
Lsp1109I GCAGC 1 cut(s) 419
LweI GCATC 2 cut(s) 196, 307
MaeIII GTNAC 2 cut(s) 58, 308
MalI GATC 5 cut(s) 182, 216, 237, 362, 456
MbiI CCGCTC 1 cut(s) 71
MboI GATC 5 cut(s) 180, 214, 235, 360, 454
MboII GAAGA 2 cut(s) 386, 491
MflI RGATCY 1 cut(s) 360
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 3 cut(s) 235, 263, 328
MseI TTAA 2 cut(s) 375, 399
MspI CCGG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 147
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 464
NdeII GATC 5 cut(s) 180, 214, 235, 360, 454
NlaIII CATG 3 cut(s) 206, 242, 269
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 2 cut(s) 58, 308
PagI TCATGA 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 492
PinAI ACCGGT 1 cut(s) 385
PkrI GCNGC 2 cut(s) 73, 409
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 145
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 3 cut(s) 128, 159, 272
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 2 cut(s) 79, 292
RsaNI GTAC 2 cut(s) 78, 291
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 2 cut(s) 375, 399
SatI GCNGC 2 cut(s) 72, 408
Sau3AI GATC 5 cut(s) 180, 214, 235, 360, 454
Sau96I GGNCC 3 cut(s) 128, 159, 272
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 147
SetI ASST 1 cut(s) 88
SfaNI GCATC 2 cut(s) 196, 307
SinI GGWCC 2 cut(s) 159, 272
SsiI CCGC 6 cut(s) 71, 74, 178, 320, 462, 472
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 3 cut(s) 170, 217, 250
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 2 cut(s) 375, 399
Tru9I TTAA 2 cut(s) 375, 399
TseFI GTSAC 2 cut(s) 58, 308
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 2 cut(s) 58, 308
TspDTI ATGAA 1 cut(s) 155
VpaK11BI GGWCC 2 cut(s) 159, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.