Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004908

Physical Location & Seq

Genomic Coordinates Forward (+)

20636289 .. 20639121

Gene Structure 2833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1091400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 774 bp

ATGTTCATCCAATCACTCCAGCCTTATAAGTACATTTGCTCTTCAATCCTCTTTTTCTTAGTTTCAAGTTTCTGCATCAACAAACACCAAGATTCCCCAACTGCCCTTTTTGCACTTTCAAGGTGCCATGATTTATTCAAGTGTATGTCTGAAGGAGAAGAACCTAAAACTCAAAAAGCTCCCAAATTGAATGAGAGGATCCTTTCATCTCTGTCAAGAAGATCAGTTGCTGCACATCCTTGGCATGATCTTGAAATTGGACCTAGTGCACCCGATATTTTCAACGTTGTTATCGAGATAACAAAGGGAAGCAAAGTCAAATATGAACTTGACAAGAAGACAGGAATGATTAAGGTTGATCGGATTTTATACTCGTCGGTGGTGTATCCTCACAATTACGGCTTCATCCCTCGCACCTTGTGTGAAGACAATGATCCACTGGATGTTTTAGTCCTCATGCAGGAACCTGTGCTTCCGGGTTGCTTTCTGCGAGCCAGAGCCATTGGAGTGATGCCCATGATTGATCAGGGAGAGAAGGATGATAAGATCATCGCAGTCTGCGCCGATGATCCAGAGTACACACATTACACTGCACTCGATGACCTCCCCCCTCATCGCCTTTCTGAGATTCGTCGCTTCTTTGAAGACTACAAGAAGAATGAGAACAAAGAGGTTGCAGTAAACGCCTTCTTGCCCGCTAGCACTGCTCTTGAAGCTATCCAGTACTCAATGGATCTTTATGCTGAGTACATAATGCACACCTTAAGGCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004427 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0008150 GO:0010876 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0019915 GO:0033036 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051235 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Pyrophosphatase

Protein Analysis

258

Amino Acids

29.43

Weight (kDa)

5.92

Isoelectric Point (pI)

56.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pyrophosphatase	PF00719	96 - 247	2.7e-59	Inorganic pyrophosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0004908)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01050 AT1G01050 AT2G46860 AT4G01480 AT4G01480
fragaria_vesca	FvH4_7g17871 FvH4_7g17880 FvH4_7g17880 FvH4_7g17880
malus_domestica	MD01G1091200.v1.1 MD01G1091400.v1.1 MD07G1161500.v1.1
prunus_persica	Prupe.2G200700_v2.0.a1 Prupe.2G200700_v2.0.a1 Prupe.2G200800_v2.0.a1 Prupe.2G200800_v2.0.a1
pyrus_communis	pycom01g11550
rosa_chinensis	RchiOBHm_Chr1g0361351
rosa_laevigata	RLG00000027723 RLG00000027724
rosa_multiflora	Rmu_sc0002119.1_g000027
rosa_roxburghii	Rroxscaffold_4G00295030
rosa_rugosa	Rorug01G0288300 Rorug01G0288400 Rorug01G0288500 Rorug01G0288800
rosa_samantha	Rh1AG299600 Rh1BG263400
rosa_wichuraiana	Rw1G026520 Rw1G026530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	27
AccB1I	GGYRCC	1 cut(s)	123
AciI	CCGC	2 cut(s)	696, 769
AclI	AACGTT	1 cut(s)	285
AclWI	GGATC	5 cut(s)	193, 206, 428, 563, 741
AcuI	CTGAAG	1 cut(s)	171
AdeI	CACNNNGTG	1 cut(s)	420
AfaI	GTAC	4 cut(s)	32, 578, 725, 749
AfiI	CCNNNNNNNGG	2 cut(s)	460, 768
AflII	CTTAAG	1 cut(s)	763
AgsI	TTSAA	9 cut(s)	45, 66, 120, 139, 190, 254, 283, 644, 713
AluBI	AGCT	2 cut(s)	179, 716
AluI	AGCT	2 cut(s)	179, 716
Alw21I	GWGCWC	1 cut(s)	271
Alw44I	GTGCAC	1 cut(s)	267
AlwI	GGATC	5 cut(s)	193, 206, 428, 563, 741
AlwNI	CAGNNNCTG	1 cut(s)	230
ApaLI	GTGCAC	1 cut(s)	267
ApeKI	GCWGC	1 cut(s)	230
AspLEI	GCGC	1 cut(s)	563
AspS9I	GGNCC	1 cut(s)	260
AsuC2I	CCSGG	1 cut(s)	477
AsuNHI	GCTAGC	1 cut(s)	698
AvaII	GGWCC	1 cut(s)	260
BaeGI	GKGCMC	1 cut(s)	271
BamHI	GGATCC	1 cut(s)	198
BanI	GGYRCC	1 cut(s)	123
BbsI	GAAGAC	3 cut(s)	344, 432, 651
Bbv12I	GWGCWC	1 cut(s)	271
BbvI	GCAGC	1 cut(s)	217
BceAI	ACGGC	1 cut(s)	415
BciVI	GTATCC	1 cut(s)	396
BclI	TGATCA	1 cut(s)	523
BcnI	CCSGG	1 cut(s)	477
BfaI	CTAG	2 cut(s)	264, 699
BfrI	CTTAAG	1 cut(s)	763
BfuI	GTATCC	1 cut(s)	396
BisI	GCNGC	1 cut(s)	231
BlsI	GCNGC	1 cut(s)	232
BmcAI	AGTACT	1 cut(s)	725
Bme1390I	CCNGG	1 cut(s)	477
Bme18I	GGWCC	1 cut(s)	260
BmgT120I	GGNCC	1 cut(s)	260
BmiI	GGNNCC	3 cut(s)	125, 200, 465
BmrFI	CCNGG	1 cut(s)	477
BmsI	GCATC	2 cut(s)	84, 501
BmtI	GCTAGC	1 cut(s)	702
BpiI	GAAGAC	3 cut(s)	344, 432, 651
BpuMI	CCSGG	1 cut(s)	477
BsaJI	CCNNGG	1 cut(s)	239
Bsc4I	CCNNNNNNNGG	2 cut(s)	460, 768
Bse1I	ACTGG	2 cut(s)	444, 721
BseDI	CCNNGG	1 cut(s)	239
BseGI	GGATG	5 cut(s)	6, 235, 405, 448, 544
BseLI	CCNNNNNNNGG	2 cut(s)	460, 768
BseMII	CTCAG	2 cut(s)	615, 735
BseNI	ACTGG	2 cut(s)	444, 721
BseSI	GKGCMC	1 cut(s)	271
BseXI	GCAGC	1 cut(s)	217
BsgI	GTGCAG	2 cut(s)	216, 576
BshNI	GGYRCC	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	271
BsiSI	CCGG	1 cut(s)	476
BslI	CCNNNNNNNGG	2 cut(s)	460, 768
Bsp1286I	GDGCHC	1 cut(s)	271
Bsp143I	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
BspACI	CCGC	2 cut(s)	696, 769
BspCNI	CTCAG	2 cut(s)	616, 736
BspLI	GGNNCC	3 cut(s)	125, 200, 465
BspOI	GCTAGC	1 cut(s)	702
BspPI	GGATC	5 cut(s)	193, 206, 428, 563, 741
BspQI	GCTCTTC	1 cut(s)	46
BspT107I	GGYRCC	1 cut(s)	123
BspTI	CTTAAG	1 cut(s)	763
BsrI	ACTGG	2 cut(s)	444, 721
BssECI	CCNNGG	1 cut(s)	239
BssMI	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
BssT1I	CCWWGG	1 cut(s)	239
Bst6I	CTCTTC	1 cut(s)	46
BstAFI	CTTAAG	1 cut(s)	763
BstC8I	GCNNGC	3 cut(s)	492, 696, 700
BstDEI	CTNAG	3 cut(s)	58, 624, 744
BstENI	CCTNNNNNAGG	1 cut(s)	458
BstF5I	GGATG	5 cut(s)	6, 235, 405, 448, 544
BstHHI	GCGC	1 cut(s)	563
BstKTI	GATC	9 cut(s)	201, 224, 250, 361, 436, 526, 549, 571, 736
BstMBI	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
BstMWI	GCNNNNNNNGC	5 cut(s)	110, 560, 683, 704, 713
BstSCI	CCNGG	1 cut(s)	475
BstSLI	GKGCMC	1 cut(s)	271
BstV1I	GCAGC	1 cut(s)	217
BstV2I	GAAGAC	3 cut(s)	344, 432, 651
BstX2I	RGATCY	2 cut(s)	198, 733
BstYI	RGATCY	2 cut(s)	198, 733
BsuI	GTATCC	1 cut(s)	396
BtgZI	GCGATG	2 cut(s)	535, 599
BtsCI	GGATG	5 cut(s)	6, 235, 405, 448, 544
BtsI	GCAGTG	2 cut(s)	588, 702
BtsIMutI	CAGTG	3 cut(s)	437, 588, 702
Cac8I	GCNNGC	3 cut(s)	492, 696, 700
CaiI	CAGNNNCTG	1 cut(s)	230
CfoI	GCGC	1 cut(s)	563
Cfr13I	GGNCC	1 cut(s)	260
Csp6I	GTAC	4 cut(s)	31, 577, 724, 748
CviAII	CATG	4 cut(s)	128, 245, 457, 517
CviJI	RGCY	6 cut(s)	22, 179, 402, 494, 500, 716
CviKI_1	RGCY	6 cut(s)	22, 179, 402, 494, 500, 716
CviQI	GTAC	4 cut(s)	31, 577, 724, 748
DdeI	CTNAG	3 cut(s)	58, 624, 744
DpnI	GATC	9 cut(s)	200, 223, 249, 360, 435, 525, 548, 570, 735
DpnII	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
DraIII	CACNNNGTG	1 cut(s)	420
Eam1104I	CTCTTC	1 cut(s)	46
EarI	CTCTTC	1 cut(s)	46
Eco130I	CCWWGG	1 cut(s)	239
Eco47I	GGWCC	1 cut(s)	260
Eco57I	CTGAAG	1 cut(s)	171
EcoNI	CCTNNNNNAGG	1 cut(s)	458
EcoT14I	CCWWGG	1 cut(s)	239
ErhI	CCWWGG	1 cut(s)	239
FaeI	CATG	4 cut(s)	131, 248, 460, 520
FatI	CATG	4 cut(s)	127, 244, 456, 516
FauI	CCCGC	1 cut(s)	703
FbaI	TGATCA	1 cut(s)	523
Fnu4HI	GCNGC	1 cut(s)	231
FokI	GGATG	4 cut(s)	222, 392, 455, 551
Fsp4HI	GCNGC	1 cut(s)	231
FspBI	CTAG	2 cut(s)	264, 699
GlaI	GCGC	1 cut(s)	562
GluI	GCNGC	1 cut(s)	231
HapII	CCGG	1 cut(s)	476
HhaI	GCGC	1 cut(s)	563
Hin1II	CATG	4 cut(s)	131, 248, 460, 520
Hin6I	GCGC	1 cut(s)	561
HinP1I	GCGC	1 cut(s)	561
HinfI	GANTC	2 cut(s)	92, 628
HpaII	CCGG	1 cut(s)	476
Hpy166II	GTNNAC	3 cut(s)	269, 579, 682
Hpy188I	TCNGA	3 cut(s)	151, 363, 625
Hpy188III	TCNNGA	5 cut(s)	216, 251, 295, 572, 710
Hpy8I	GTNNAC	3 cut(s)	269, 579, 682
Hpy99I	CGWCG	2 cut(s)	379, 636
HpyAV	CCTTC	3 cut(s)	146, 529, 697
HpyCH4IV	ACGT	1 cut(s)	285
HpyCH4V	TGCA	8 cut(s)	75, 113, 233, 269, 460, 593, 677, 757
HpyF10VI	GCNNNNNNNGC	5 cut(s)	110, 560, 683, 704, 713
HpyF3I	CTNAG	3 cut(s)	58, 624, 744
HpySE526I	ACGT	1 cut(s)	285
Hsp92II	CATG	4 cut(s)	131, 248, 460, 520
HspAI	GCGC	1 cut(s)	561
Ksp22I	TGATCA	1 cut(s)	523
Kzo9I	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
LguI	GCTCTTC	1 cut(s)	46
LmnI	GCTCC	1 cut(s)	184
Lsp1109I	GCAGC	1 cut(s)	217
LweI	GCATC	2 cut(s)	84, 501
MaeI	CTAG	2 cut(s)	264, 699
MaeII	ACGT	1 cut(s)	285
MalI	GATC	9 cut(s)	200, 223, 249, 360, 435, 525, 548, 570, 735
MboI	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
MboII	GAAGA	7 cut(s)	33, 170, 231, 349, 437, 656, 667
MflI	RGATCY	2 cut(s)	198, 733
MhlI	GDGCHC	1 cut(s)	271
MluCI	AATT	3 cut(s)	185, 255, 394
MnlI	CCTC	8 cut(s)	59, 189, 399, 420, 464, 614, 621, 664
MseI	TTAA	2 cut(s)	351, 764
MslI	CAYNNNNRTG	1 cut(s)	506
MspCI	CTTAAG	1 cut(s)	763
MspI	CCGG	1 cut(s)	476
MspR9I	CCNGG	1 cut(s)	477
MwoI	GCNNNNNNNGC	5 cut(s)	110, 560, 683, 704, 713
NciI	CCSGG	1 cut(s)	477
NdeII	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
NheI	GCTAGC	1 cut(s)	698
NlaIII	CATG	4 cut(s)	131, 248, 460, 520
NlaIV	GGNNCC	3 cut(s)	125, 200, 465
PciSI	GCTCTTC	1 cut(s)	46
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PfeI	GAWTC	2 cut(s)	92, 628
PkrI	GCNGC	1 cut(s)	232
PsiI	TTATAA	1 cut(s)	27
Psp1406I	AACGTT	1 cut(s)	285
PspN4I	GGNNCC	3 cut(s)	125, 200, 465
PspPI	GGNCC	1 cut(s)	260
PstNI	CAGNNNCTG	1 cut(s)	230
PsuI	RGATCY	2 cut(s)	198, 733
RsaI	GTAC	4 cut(s)	32, 578, 725, 749
RsaNI	GTAC	4 cut(s)	31, 577, 724, 748
RseI	CAYNNNNRTG	1 cut(s)	506
SapI	GCTCTTC	1 cut(s)	46
SaqAI	TTAA	2 cut(s)	351, 764
SatI	GCNGC	1 cut(s)	231
Sau3AI	GATC	9 cut(s)	198, 221, 247, 358, 433, 523, 546, 568, 733
Sau96I	GGNCC	1 cut(s)	260
ScaI	AGTACT	1 cut(s)	725
ScrFI	CCNGG	1 cut(s)	477
SduI	GDGCHC	1 cut(s)	271
SfaNI	GCATC	2 cut(s)	84, 501
SinI	GGWCC	1 cut(s)	260
SmiMI	CAYNNNNRTG	1 cut(s)	506
SmlI	CTYRAG	1 cut(s)	763
SmoI	CTYRAG	1 cut(s)	763
Sse9I	AATT	3 cut(s)	185, 255, 394
SsiI	CCGC	2 cut(s)	696, 769
SspMI	CTAG	2 cut(s)	264, 699
StyD4I	CCNGG	1 cut(s)	475
StyI	CCWWGG	1 cut(s)	239
TaiI	ACGT	1 cut(s)	288
TaqI	TCGA	2 cut(s)	294, 597
TasI	AATT	3 cut(s)	185, 255, 394
TatI	WGTACW	4 cut(s)	30, 576, 723, 747
TfiI	GAWTC	2 cut(s)	92, 628
Tru1I	TTAA	2 cut(s)	351, 764
Tru9I	TTAA	2 cut(s)	351, 764
TscAI	CASTG	3 cut(s)	444, 595, 709
TseI	GCWGC	1 cut(s)	230
TspDTI	ATGAA	3 cut(s)	195, 339, 394
TspRI	CASTG	3 cut(s)	444, 595, 709
Vha464I	CTTAAG	1 cut(s)	763
VneI	GTGCAC	1 cut(s)	267
VpaK11BI	GGWCC	1 cut(s)	260
XagI	CCTNNNNNAGG	1 cut(s)	458
XspI	CTAG	2 cut(s)	264, 699
ZrmI	AGTACT	1 cut(s)	725

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.