Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001912

Physical Location & Seq

Genomic Coordinates Reverse (-)

21528166 .. 21536320

Gene Structure 8155 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1103200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3225 bp

ATGGAAGTAACAGTACGGGTGGAAGGTCCCAGAGGCAAACGACATCGTCGGCGCAAGTCCCTCTTACTCACTCCACTAGACAAACGACGTCGTCTTTGCAAGCCAACAGCCACCGCACACGGCCCTCTTCTTCCAGTTCACGGCGCTCCGTCATACCCAGTTCGAGAACTCCTCACTCTAACCCCGAATATCAACAAGGCCGGAAGAGTGAGGGTTGCTGTCAGGCTTCGACCGAGAAATCTGGAGGATCGCATTTCGGATTCTGATTTCGCAGACATTGTCGAGTTGCAGCCCGAGCTGAAGCGGCTAAAGTTGAGAAAGAACAATTGGAGCTCAGAGTCTTACAGATTTGATGAAGTTTTCACTGAAACCGCCTCACAAAATCGGGTTTATCAAGTGGTGGCAAAGCCTGTAGTTGAGAGTGTGTTGAGTGGATATAATGGGACAGTTATGGCCTATGGCCAAACCGGTACTGGGAAAACTTACACAGTTGGCAGTCTGGGTAATGATGATGCATCCGAGCGTGGCATTATGGTTAGAGCTTTGGAGGACATACTTTCGACTACATCTACTGCTTTTGATAGTGTGGAAGTTTCCTACTTGCAGTTGTACATGGAATCTATTCAAGATTTGCTTGCACCAGAAAAGATTAATATTCCTATTCATGAGGACTCGAAGACTGGTGAGGTGTCACTTCCTGGTGCTTCAGTAGTAAGAATTAAAGACCTTGATCACTTTTTACAACTGCTGCAAATTGGTGAGGCAAACCGTCATGCAGCTAACACGAAGCTGAATACAGAATCTTCACGCAGTCACGCGATTCTTATGGTTTTTGTTCGTAGATCTGTGCAAGAAAATGCTACAGATGAGATCACTTCTCAAGAAAAAACCTTTAGAGCTGATTTATCAGTTAGAAGTGGGGTACCTGTAGTTCGAAAAAGCAAGTTGTTGATTGTTGATCTTGCAGGATCTGAAAGAATAGATAAATCTGGCAGTGAGGGCCACTTGCTTGAGGAGGCGAAATTTATAAATCTTTCACTTACTTCCCTTGGAAAATGTATAAATGCATTGGCGGAAAACAGTCCACATATCCCTGTCAGAGATTCTAAGCTTACAAGGTTGCTTCGTGATTCATTTGGAGGCTCTGCGAGGACCTCACTCATAGTAATGATTGGGCTGTCAGCACAGCACTATGCAGAGACCACTAGTACAATAATGTTTGGACAACGGGCAATGAGAATAGTAAATATGGTAAAACTTAAAGAAGAATTTGATTATGAAAGTCAATGTCGGAAGCTTGAGAATCAAGTAGACCATCTTACTGCACAATTTGAAAGGCAACAGAAGTTGTTGGACAGTAACAAATTGGAATTGGAAAGACAGCTGAAGGAGTGTCAAGATTCTTTTGCTGAGGCCAAGAACAATCTAAGCACAAGGTCTGAGTTATTGGAGAAGGAAAATGCTCGTTTGGAGTCGGATCTGAATGATCTTTTAGATGAGCTGAACTGTCATAAAGACCACAGTGAGTTGATGCATGATAAGGTTGCAAAGCTTGAAGCAAGTTTAGAACATGGAGAGTTTCTGGAGAAGGAAAATACTCGTTTGGAGTTGGATCTGAAAGATCTTTCAGATAAACTGAACCTTGAGAAAGAACACAGTGACCTGATGCATGATAAAGTTGCAGAACTTGAAGCAAGTTTAGAACACGGCAAGCAACACCAGCTTGAAAGTTCTATGTATCAGAAAGTCCTAGCTGATAGCACTCAGATGTATGAAGAGAAAATAGCAAAGTTGATTAAGCAGCTAGATGATGAGCATGCTCGTGCTGAAAGCACAGAGGAACAGTTAGATTTAACAACGAAGCTTCTGACTGATGGCCAAAAGACTATTCAGCAACATCAGATAGAGAATTCTGCATTTCAATCAGCACTTGCAGACGCTACTCAGATGTATGAGAAAAAAATAGGTGAGTTAATGGAGCAATTGGAAAATGAGCACGCTCGTTTTGAAGATCTAGAAGAACAATTGGATGTGGAGAAGAAGCAACGTAGTCGGCAAAACTCAATGCAGGGGAAGAAAGAGATTGATGAACTTAGAGTGCAGTTAGAAGAAATGAATCAGCTACATGAACAAACTCTAAACAAATATTGGTCCTTGAAATCAGAATGTGCTGATCTCTCAGAAGAGAAGGAAATACTCAAGGAAGAACTTCAAGATGTGAGGCAAAGACTTCTAATTGAAGAGAAGCAGAGGAAGTCTGTAGAGAATGAGCTAATCAAATTAAAGAAGGCTGCACCGGAGAAAGAAGATGAGTTTGAGGACAAGAAATTGTATATGAAGGAAAATACACATAAAGGATCTTCTGCTTTGGGGAATCCAATGGGGTTACACAATGCAAATCCATCGAGAGATACAATTTCTGGTCAGAGGGCCACAATTGCAAAAATATGTGAAGAAGTTGGGCTTCAAAAGATTGTACAATTGTTGACATCTGAAGATTCAGATGTCCAAACCCATGCTGTGAAGGTGGTGGCCAATCTTGCAGCTGAAGATACTAATCAAGCAAAGATTGTTGAGGAAGGCGGTTTAGACGCTTTGCTTGTTCTGTTGCAATCCTCACAAAATACAACTATCCTTAGAGTTGCTTCTGGGGCCATTGCCAATTTGGCAATGAATGAGGTAAATCAAGGCGCAATTATGAGCAGAGGCGGAGCTCAACTACTCGCAGAGACAGCATGCAAGACAAATGATCCACAAACTCTTAGAATGGTTGCTGGTGCACTTGCTAATTTATGTGGAAATGAAAGATTACATACAATGCTGAAAGAAGATGGAGGAATTAAGGCACTCTTGGGAATGGGGAGGTCGGGGAGTAGTGATGTCATTGCACAGGTTGCCAGAGGACTGGCTAACTTTGCAAAATGTGAATCGCGCGGAACTATTCAAGGACATAGAAAAGGTCGTTCACTTTTGATGGACGATGGTGCCCTTACCTGGTTGATTATCAACTCCAACACTGCCTCAACTTCAACCCGACGCCATATAGAGCTTGCTCTCTGCCATTTAGCGCAAAACGAGGACAATGCAAGGGATTTCATATCCGGTGGAGGGCTGCAAGAGATCGTTCGAATATCAACTGAATCCATTCGGGACGATATCCGAAACTTGGCAAAGAAGGCGTTGAGAGTGAATCCGAAACTTTACAATCAGAGGCACGGTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

1075

Amino Acids

120.52

Weight (kDa)

5.88

Isoelectric Point (pI)

43.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	67 - 212	5.7e-18	Microtubule binding
Kinesin	PF00225	75 - 414	1.4e-95	Kinesin motor domain
ARM_ARMC5	PF24768	816 - 920	8.2e-09	ARMC5
ARM_ARMC5	PF24768	843 - 979	2.4e-11	ARMC5
Arm	PF00514	855 - 890	5.2e-06	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54870 AT3G54870 AT3G54870
fragaria_vesca	FvH4_2g41290 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790
malus_domestica	MD01G1103200.v1.1 MD07G1169900.v1.1
prunus_persica	Prupe.2G208800_v2.0.a1 Prupe.2G208800_v2.0.a1 Prupe.2G208800_v2.0.a1
pyrus_communis	pycom01g12970 pycom07g16380
rosa_chinensis	RchiOBHm_Chr1g0362591 RchiOBHm_Chr3g0488101
rosa_laevigata	RLG00000027636
rosa_multiflora	Rmu_co8281713.1_g000001 Rmu_sc0001716.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043680 Rroxscaffold_2G00076710 Rroxscaffold_3G00218650 Rroxscaffold_4G00293920 Rroxscaffold_4G00293930
rosa_rugosa	Rorug01G0299900.1
rosa_samantha	Rh1AG308500 Rh1BG272200 Rh1CG289400 Rh1DG302600 Rh2AG571000 Rh2BG101900 Rh2CG104300 Rh3AG286300 Rh5AG442000 Rh5BG250100 Rh5BG250200 Rh5BG276500
rosa_wichuraiana	Rw1G008860 Rw1G027400 Rw6G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1028
AatII	GACGTC	1 cut(s)	91
Acc65I	GGTACC	1 cut(s)	922
AccB1I	GGYRCC	2 cut(s)	922, 2984
AccI	GTMKAC	1 cut(s)	1311
AccII	CGCG	3 cut(s)	818, 2932, 2934
AciI	CCGC	7 cut(s)	114, 304, 372, 1073, 2583, 2709, 2934
AclWI	GGATC	6 cut(s)	255, 976, 1485, 1620, 2365, 2744
AcoI	YGGCCR	3 cut(s)	460, 1876, 2532
AcsI	RAATTY	3 cut(s)	1022, 1268, 1909
AcuI	CTGAAG	5 cut(s)	320, 690, 1406, 2514, 2568
AcyI	GRCGYC	2 cut(s)	88, 3037
AfaI	GTAC	6 cut(s)	15, 472, 611, 924, 1210, 2478
AfiI	CCNNNNNNNGG	5 cut(s)	140, 474, 2994, 3108, 3166
AgeI	ACCGGT	1 cut(s)	467
AhlI	ACTAGT	1 cut(s)	1205
AjnI	CCWGG	2 cut(s)	697, 2993
AjuI	GAANNNNNNNTTGG	4 cut(s)	310, 342, 1872, 1904
Alw21I	GWGCWC	4 cut(s)	335, 1998, 2716, 2782
Alw26I	GTCTC	2 cut(s)	1193, 2723
Alw44I	GTGCAC	1 cut(s)	2778
AlwI	GGATC	6 cut(s)	255, 976, 1485, 1620, 2365, 2744
AlwNI	CAGNNNCTG	1 cut(s)	971
Ama87I	CYCGRG	1 cut(s)	293
ApaLI	GTGCAC	1 cut(s)	2778
ApeKI	GCWGC	7 cut(s)	289, 748, 776, 1801, 2291, 2543, 3112
ApoI	RAATTY	3 cut(s)	1022, 1268, 1909
ArsI	GACNNNNNNTTYG	2 cut(s)	78, 110
AseI	ATTAAT	1 cut(s)	651
AsiGI	ACCGGT	1 cut(s)	467
Asp700I	GAANNNNTTC	3 cut(s)	621, 2208, 3144
Asp718I	GGTACC	1 cut(s)	922
AspLEI	GCGC	5 cut(s)	54, 146, 2693, 2934, 3070
AspS9I	GGNCC	7 cut(s)	26, 122, 1000, 1152, 2151, 2430, 2652
AsuHPI	GGTGA	3 cut(s)	695, 770, 1979
AsuII	TTCGAA	2 cut(s)	934, 3127
AvaI	CYCGRG	1 cut(s)	293
AvaII	GGWCC	3 cut(s)	26, 1152, 2151
BaeGI	GKGCMC	2 cut(s)	2782, 2989
BalI	TGGCCA	3 cut(s)	462, 1878, 2534
BanI	GGYRCC	2 cut(s)	922, 2984
BanII	GRGCYC	2 cut(s)	335, 2716
BarI	GAAGNNNNNNTAC	1 cut(s)	29
BauI	CACGAG	1 cut(s)	1821
BbsI	GAAGAC	1 cut(s)	683
Bbv12I	GWGCWC	4 cut(s)	335, 1998, 2716, 2782
BbvCI	CCTCAGC	1 cut(s)	1410
BbvI	GCAGC	7 cut(s)	301, 735, 788, 1813, 2278, 2555, 3099
BccI	CCATC	6 cut(s)	1323, 1868, 2410, 2825, 2968, 2975
BceAI	ACGGC	3 cut(s)	136, 157, 1723
BcgI	CGANNNNNNTGC	6 cut(s)	2033, 2067, 2385, 2419, 3065, 3099
BciT130I	CCWGG	2 cut(s)	699, 2995
BclI	TGATCA	1 cut(s)	730
BcoDI	GTCTC	2 cut(s)	1193, 2723
BcuI	ACTAGT	1 cut(s)	1205
BfaI	CTAG	5 cut(s)	77, 1206, 1751, 1805, 2015
BfmI	CTRYAG	4 cut(s)	411, 861, 927, 2259
BfoI	RGCGCY	1 cut(s)	147
BglI	GCCNNNNNGGC	1 cut(s)	2666
BglII	AGATCT	3 cut(s)	842, 1621, 2011
BisI	GCNGC	8 cut(s)	290, 305, 749, 777, 1802, 2292, 2544, 3113
BlsI	GCNGC	8 cut(s)	291, 306, 750, 778, 1803, 2293, 2545, 3114
Bme1390I	CCNGG	2 cut(s)	699, 2995
Bme18I	GGWCC	3 cut(s)	26, 1152, 2151
BmeT110I	CYCGRG	1 cut(s)	293
BmgT120I	GGNCC	7 cut(s)	26, 122, 1000, 1152, 2151, 2430, 2652
BmiI	GGNNCC	4 cut(s)	28, 924, 2653, 2986
BmrFI	CCNGG	2 cut(s)	699, 2995
BmrI	ACTGGG	2 cut(s)	152, 483
BmsI	GCATC	4 cut(s)	502, 524, 1521, 1656
BmuI	ACTGGG	2 cut(s)	152, 483
BpiI	GAAGAC	1 cut(s)	683
BplI	GAGNNNNNCTC	2 cut(s)	156, 188
BpmI	CTGGAG	2 cut(s)	263, 1604
Bpu10I	CCTNAGC	1 cut(s)	1410
Bpu14I	TTCGAA	2 cut(s)	934, 3127
BpuEI	CTTGAG	5 cut(s)	864, 1031, 1319, 1664, 2183
BsaBI	GATNNNNATC	1 cut(s)	2556
BsaHI	GRCGYC	2 cut(s)	88, 3037
BsaI	GGTCTC	1 cut(s)	1193
BsaJI	CCNNGG	1 cut(s)	1048
BsaWI	WCCGGW	3 cut(s)	467, 2296, 3101
Bsc4I	CCNNNNNNNGG	5 cut(s)	140, 474, 2994, 3108, 3166
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	5 cut(s)	134, 158, 478, 685, 2910
Bse3DI	GCAATG	4 cut(s)	1239, 2655, 2676, 2883
Bse8I	GATNNNNATC	1 cut(s)	2556
BseBI	CCWGG	2 cut(s)	699, 2995
BseDI	CCNNGG	1 cut(s)	1048
BseGI	GGATG	2 cut(s)	515, 2035
BseJI	GATNNNNATC	1 cut(s)	2556
BseLI	CCNNNNNNNGG	5 cut(s)	140, 474, 2994, 3108, 3166
BseMI	GCAATG	4 cut(s)	1239, 2655, 2676, 2883
BseMII	CTCAG	6 cut(s)	348, 1401, 1431, 1778, 1958, 2193
BseNI	ACTGG	5 cut(s)	134, 158, 478, 685, 2910
BseRI	GAGGAG	2 cut(s)	161, 1028
BseSI	GKGCMC	2 cut(s)	2782, 2989
BseXI	GCAGC	7 cut(s)	301, 735, 788, 1813, 2278, 2555, 3099
BsgI	GTGCAG	3 cut(s)	1308, 2120, 2277
Bsh1236I	CGCG	3 cut(s)	818, 2932, 2934
Bsh1285I	CGRYCG	1 cut(s)	233
BshNI	GGYRCC	2 cut(s)	922, 2984
BshTI	ACCGGT	1 cut(s)	467
BsiEI	CGRYCG	1 cut(s)	233
BsiHKAI	GWGCWC	4 cut(s)	335, 1998, 2716, 2782
BsiHKCI	CYCGRG	1 cut(s)	293
BsiSI	CCGG	4 cut(s)	201, 468, 2297, 3102
BslFI	GGGAC	4 cut(s)	12, 43, 457, 3164
BslI	CCNNNNNNNGG	5 cut(s)	140, 474, 2994, 3108, 3166
BsmAI	GTCTC	2 cut(s)	1193, 2723
BsmFI	GGGAC	4 cut(s)	12, 43, 457, 3164
Bso31I	GGTCTC	1 cut(s)	1193
BsoBI	CYCGRG	1 cut(s)	293
Bsp119I	TTCGAA	2 cut(s)	934, 3127
Bsp1286I	GDGCHC	5 cut(s)	335, 1998, 2716, 2782, 2989
Bsp1407I	TGTACA	2 cut(s)	609, 2476
BspACI	CCGC	7 cut(s)	114, 304, 372, 1073, 2583, 2709, 2934
BspCNI	CTCAG	6 cut(s)	347, 1402, 1432, 1777, 1957, 2192
BspFNI	CGCG	3 cut(s)	818, 2932, 2934
BspHI	TCATGA	1 cut(s)	664
BspLI	GGNNCC	4 cut(s)	28, 924, 2653, 2986
BspPI	GGATC	6 cut(s)	255, 976, 1485, 1620, 2365, 2744
BspT104I	TTCGAA	2 cut(s)	934, 3127
BspT107I	GGYRCC	2 cut(s)	922, 2984
BspTNI	GGTCTC	1 cut(s)	1193
BsrDI	GCAATG	4 cut(s)	1239, 2655, 2676, 2883
BsrFI	RCCGGY	1 cut(s)	467
BsrGI	TGTACA	2 cut(s)	609, 2476
BsrI	ACTGG	5 cut(s)	134, 158, 478, 685, 2910
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	1 cut(s)	1048
BssNI	GRCGYC	2 cut(s)	88, 3037
BssSI	CACGAG	1 cut(s)	1821
BssT1I	CCWWGG	1 cut(s)	1048
Bst2BI	CACGAG	1 cut(s)	1821
Bst2UI	CCWGG	2 cut(s)	699, 2995
Bst6I	CTCTTC	5 cut(s)	132, 199, 1770, 2178, 2235
BstACI	GRCGYC	2 cut(s)	88, 3037
BstAPI	GCANNNNNTGC	1 cut(s)	2894
BstAUI	TGTACA	2 cut(s)	609, 2476
BstBI	TTCGAA	2 cut(s)	934, 3127
BstC8I	GCNNGC	7 cut(s)	101, 636, 1712, 1818, 1998, 2737, 3051
BstF5I	GGATG	2 cut(s)	515, 2035
BstFNI	CGCG	3 cut(s)	818, 2932, 2934
BstH2I	RGCGCY	1 cut(s)	147
BstHHI	GCGC	5 cut(s)	54, 146, 2693, 2934, 3070
BstMAI	GTCTC	2 cut(s)	1193, 2723
BstMCI	CGRYCG	1 cut(s)	233
BstNI	CCWGG	2 cut(s)	699, 2995
BstNSI	RCATGY	2 cut(s)	1820, 2739
BstSCI	CCNGG	2 cut(s)	697, 2993
BstSFI	CTRYAG	4 cut(s)	411, 861, 927, 2259
BstSLI	GKGCMC	2 cut(s)	2782, 2989
BstUI	CGCG	3 cut(s)	818, 2932, 2934
BstV1I	GCAGC	7 cut(s)	301, 735, 788, 1813, 2278, 2555, 3099
BstV2I	GAAGAC	1 cut(s)	683
BstX2I	RGATCY	7 cut(s)	842, 968, 1477, 1612, 1621, 2011, 2357
BstXI	CCANNNNNNTGG	1 cut(s)	2905
BstYI	RGATCY	7 cut(s)	842, 968, 1477, 1612, 1621, 2011, 2357
BtsCI	GGATG	2 cut(s)	515, 2035
BtsI	GCAGTG	2 cut(s)	1000, 3015
BtsIMutI	CAGTG	5 cut(s)	363, 1000, 1528, 1663, 3015
Cac8I	GCNNGC	7 cut(s)	101, 636, 1712, 1818, 1998, 2737, 3051
CaiI	CAGNNNCTG	1 cut(s)	971
CciI	TCATGA	1 cut(s)	664
CfoI	GCGC	5 cut(s)	54, 146, 2693, 2934, 3070
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	7 cut(s)	26, 122, 1000, 1152, 2151, 2430, 2652
CseI	GACGC	3 cut(s)	1946, 2600, 3045
CsiI	ACCWGGT	1 cut(s)	2993
Csp6I	GTAC	6 cut(s)	14, 471, 610, 923, 1209, 2477
CspAI	ACCGGT	1 cut(s)	467
CviQI	GTAC	6 cut(s)	14, 471, 610, 923, 1209, 2477
EaeI	YGGCCR	3 cut(s)	460, 1876, 2532
Eam1104I	CTCTTC	5 cut(s)	132, 199, 1770, 2178, 2235
EarI	CTCTTC	5 cut(s)	132, 199, 1770, 2178, 2235
EciI	GGCGGA	2 cut(s)	1088, 2724
Ecl136II	GAGCTC	2 cut(s)	333, 2714
Eco130I	CCWWGG	1 cut(s)	1048
Eco24I	GRGCYC	2 cut(s)	335, 2716
Eco31I	GGTCTC	1 cut(s)	1193
Eco32I	GATATC	1 cut(s)	3157
Eco47I	GGWCC	3 cut(s)	26, 1152, 2151
Eco53kI	GAGCTC	2 cut(s)	333, 2714
Eco57I	CTGAAG	5 cut(s)	320, 690, 1406, 2514, 2568
Eco88I	CYCGRG	1 cut(s)	293
EcoICRI	GAGCTC	2 cut(s)	333, 2714
EcoO109I	RGGNCCY	2 cut(s)	26, 1152
EcoRI	GAATTC	1 cut(s)	1909
EcoRII	CCWGG	2 cut(s)	697, 2993
EcoRV	GATATC	1 cut(s)	3157
EcoT14I	CCWWGG	1 cut(s)	1048
EcoT22I	ATGCAT	4 cut(s)	517, 1069, 1536, 1671
EcoT38I	GRGCYC	2 cut(s)	335, 2716
ErhI	CCWWGG	1 cut(s)	1048
FalI	AAGNNNNNCTT	8 cut(s)	47, 79, 618, 650, 2448, 2480, 2834, 2866
FaqI	GGGAC	4 cut(s)	12, 43, 457, 3164
FbaI	TGATCA	1 cut(s)	730
FblI	GTMKAC	1 cut(s)	1311
Fnu4HI	GCNGC	8 cut(s)	290, 305, 749, 777, 1802, 2292, 2544, 3113
FokI	GGATG	2 cut(s)	502, 2042
FriOI	GRGCYC	2 cut(s)	335, 2716
Fsp4HI	GCNGC	8 cut(s)	290, 305, 749, 777, 1802, 2292, 2544, 3113
FspBI	CTAG	5 cut(s)	77, 1206, 1751, 1805, 2015
GlaI	GCGC	5 cut(s)	53, 145, 2692, 2933, 3069
GluI	GCNGC	8 cut(s)	290, 305, 749, 777, 1802, 2292, 2544, 3113
GsuI	CTGGAG	2 cut(s)	263, 1604
HaeII	RGCGCY	1 cut(s)	147
HapII	CCGG	4 cut(s)	201, 468, 2297, 3102
HgaI	GACGC	3 cut(s)	1946, 2600, 3045
HhaI	GCGC	5 cut(s)	54, 146, 2693, 2934, 3070
Hin1I	GRCGYC	2 cut(s)	88, 3037
Hin6I	GCGC	5 cut(s)	52, 144, 2691, 2932, 3068
HinP1I	GCGC	5 cut(s)	52, 144, 2691, 2932, 3068
HincII	GTYRAC	1 cut(s)	2487
HindII	GTYRAC	1 cut(s)	2487
HindIII	AAGCTT	4 cut(s)	1109, 1295, 1550, 1862
HpaII	CCGG	4 cut(s)	201, 468, 2297, 3102
HphI	GGTGA	3 cut(s)	695, 770, 1979
Hpy166II	GTNNAC	6 cut(s)	139, 1085, 1312, 2487, 2780, 2966
Hpy8I	GTNNAC	6 cut(s)	139, 1085, 1312, 2487, 2780, 2966
Hpy99I	CGWCG	4 cut(s)	51, 90, 93, 3039
HpyCH4IV	ACGT	2 cut(s)	88, 2047
HpySE526I	ACGT	2 cut(s)	88, 2047
Hsp92I	GRCGYC	2 cut(s)	88, 3037
HspAI	GCGC	5 cut(s)	52, 144, 2691, 2932, 3068
KpnI	GGTACC	1 cut(s)	926
Ksp22I	TGATCA	1 cut(s)	730
LmnI	GCTCC	4 cut(s)	151, 330, 1978, 2711
Lsp1109I	GCAGC	7 cut(s)	301, 735, 788, 1813, 2278, 2555, 3099
LweI	GCATC	4 cut(s)	502, 524, 1521, 1656
MabI	ACCWGGT	1 cut(s)	2993
MaeI	CTAG	5 cut(s)	77, 1206, 1751, 1805, 2015
MaeII	ACGT	2 cut(s)	88, 2047
MaeIII	GTNAC	6 cut(s)	7, 690, 812, 1358, 1658, 2385
MfeI	CAATTG	5 cut(s)	325, 1982, 2024, 2436, 2480
MflI	RGATCY	7 cut(s)	842, 968, 1477, 1612, 1621, 2011, 2357
MhlI	GDGCHC	5 cut(s)	335, 1998, 2716, 2782, 2989
MlsI	TGGCCA	3 cut(s)	462, 1878, 2534
MluNI	TGGCCA	3 cut(s)	462, 1878, 2534
MlyI	GAGTC	3 cut(s)	347, 665, 1481
MmeI	TCCRAC	5 cut(s)	1271, 1332, 1455, 1590, 3036
Mox20I	TGGCCA	3 cut(s)	462, 1878, 2534
Mph1103I	ATGCAT	4 cut(s)	517, 1069, 1536, 1671
MroXI	GAANNNNTTC	3 cut(s)	621, 2208, 3144
MscI	TGGCCA	3 cut(s)	462, 1878, 2534
MseI	TTAA	8 cut(s)	651, 720, 1260, 1797, 1853, 1973, 2282, 2841
MslI	CAYNNNNRTG	3 cut(s)	1166, 1766, 1821
Msp20I	TGGCCA	3 cut(s)	462, 1878, 2534
MspA1I	CMGCKG	2 cut(s)	1384, 2546
MspI	CCGG	4 cut(s)	201, 468, 2297, 3102
MspR9I	CCNGG	2 cut(s)	699, 2995
MunI	CAATTG	5 cut(s)	325, 1982, 2024, 2436, 2480
MvaI	CCWGG	2 cut(s)	699, 2995
MvnI	CGCG	3 cut(s)	818, 2932, 2934
NlaIV	GGNNCC	4 cut(s)	28, 924, 2653, 2986
NmuCI	GTSAC	3 cut(s)	690, 812, 1658
NsiI	ATGCAT	4 cut(s)	517, 1069, 1536, 1671
NspI	RCATGY	2 cut(s)	1820, 2739
NspV	TTCGAA	2 cut(s)	934, 3127
PaeI	GCATGC	2 cut(s)	1820, 2739
PagI	TCATGA	1 cut(s)	664
PdmI	GAANNNNTTC	3 cut(s)	621, 2208, 3144
PflFI	GACNNNGTC	3 cut(s)	45, 90, 278
PinAI	ACCGGT	1 cut(s)	467
PkrI	GCNGC	8 cut(s)	291, 306, 750, 778, 1803, 2293, 2545, 3114
PleI	GAGTC	3 cut(s)	346, 665, 1480
PpsI	GAGTC	3 cut(s)	346, 665, 1480
PpuMI	RGGWCCY	2 cut(s)	26, 1152
PshBI	ATTAAT	1 cut(s)	651
PsiI	TTATAA	1 cut(s)	1028
Psp124BI	GAGCTC	2 cut(s)	335, 2716
Psp5II	RGGWCCY	2 cut(s)	26, 1152
Psp6I	CCWGG	2 cut(s)	697, 2993
PspGI	CCWGG	2 cut(s)	697, 2993
PspN4I	GGNNCC	4 cut(s)	28, 924, 2653, 2986
PspPI	GGNCC	7 cut(s)	26, 122, 1000, 1152, 2151, 2430, 2652
PspPPI	RGGWCCY	2 cut(s)	26, 1152
PsrI	GAACNNNNNNTAC	2 cut(s)	915, 947
PstNI	CAGNNNCTG	1 cut(s)	971
PsuI	RGATCY	7 cut(s)	842, 968, 1477, 1612, 1621, 2011, 2357
PsyI	GACNNNGTC	3 cut(s)	45, 90, 278
PvuII	CAGCTG	2 cut(s)	1384, 2546
RsaI	GTAC	6 cut(s)	15, 472, 611, 924, 1210, 2478
RsaNI	GTAC	6 cut(s)	14, 471, 610, 923, 1209, 2477
RseI	CAYNNNNRTG	3 cut(s)	1166, 1766, 1821
SacI	GAGCTC	2 cut(s)	335, 2716
SaqAI	TTAA	8 cut(s)	651, 720, 1260, 1797, 1853, 1973, 2282, 2841
SatI	GCNGC	8 cut(s)	290, 305, 749, 777, 1802, 2292, 2544, 3113
Sau96I	GGNCC	7 cut(s)	26, 122, 1000, 1152, 2151, 2430, 2652
SchI	GAGTC	3 cut(s)	347, 665, 1481
ScrFI	CCNGG	2 cut(s)	699, 2995
SduI	GDGCHC	5 cut(s)	335, 1998, 2716, 2782, 2989
SexAI	ACCWGGT	1 cut(s)	2993
SfaNI	GCATC	4 cut(s)	502, 524, 1521, 1656
SfcI	CTRYAG	4 cut(s)	411, 861, 927, 2259
SfuI	TTCGAA	2 cut(s)	934, 3127
SinI	GGWCC	3 cut(s)	26, 1152, 2151
SmiMI	CAYNNNNRTG	3 cut(s)	1166, 1766, 1821
SmlI	CTYRAG	5 cut(s)	879, 1010, 1298, 1643, 2198
SmoI	CTYRAG	5 cut(s)	879, 1010, 1298, 1643, 2198
SpeI	ACTAGT	1 cut(s)	1205
SphI	GCATGC	2 cut(s)	1820, 2739
SsiI	CCGC	7 cut(s)	114, 304, 372, 1073, 2583, 2709, 2934
SspI	AATATT	2 cut(s)	655, 2147
SspMI	CTAG	5 cut(s)	77, 1206, 1751, 1805, 2015
SstI	GAGCTC	2 cut(s)	335, 2716
StyD4I	CCNGG	2 cut(s)	697, 2993
StyI	CCWWGG	1 cut(s)	1048
TaiI	ACGT	2 cut(s)	91, 2050
TaqI	TCGA	8 cut(s)	163, 229, 282, 560, 674, 934, 2405, 3127
TaqII	GACCGA	1 cut(s)	247
TatI	WGTACW	3 cut(s)	609, 1208, 2476
TauI	GCSGC	1 cut(s)	307
Tru1I	TTAA	8 cut(s)	651, 720, 1260, 1797, 1853, 1973, 2282, 2841
Tru9I	TTAA	8 cut(s)	651, 720, 1260, 1797, 1853, 1973, 2282, 2841
TscAI	CASTG	5 cut(s)	370, 1000, 1528, 1663, 3022
TseFI	GTSAC	3 cut(s)	690, 812, 1658
TseI	GCWGC	7 cut(s)	289, 748, 776, 1801, 2291, 2543, 3112
Tsp45I	GTSAC	3 cut(s)	690, 812, 1658
TspGWI	ACGGA	1 cut(s)	138
TspRI	CASTG	5 cut(s)	370, 1000, 1528, 1663, 3022
Tth111I	GACNNNGTC	3 cut(s)	45, 90, 278
VneI	GTGCAC	1 cut(s)	2778
VpaK11BI	GGWCC	3 cut(s)	26, 1152, 2151
VspI	ATTAAT	1 cut(s)	651
XapI	RAATTY	3 cut(s)	1022, 1268, 1909
XbaI	TCTAGA	1 cut(s)	2014
XceI	RCATGY	2 cut(s)	1820, 2739
XcmI	CCANNNNNNNNNTGG	2 cut(s)	470, 2662
XmiI	GTMKAC	1 cut(s)	1311
XmnI	GAANNNNTTC	3 cut(s)	621, 2208, 3144
XspI	CTAG	5 cut(s)	77, 1206, 1751, 1805, 2015
ZraI	GACGTC	1 cut(s)	89
Zsp2I	ATGCAT	4 cut(s)	517, 1069, 1536, 1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.