Rh5BG250100

Armadillo repeat-containing kinesin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001912
Physical Location & Seq
Reverse (-)
30641335 .. 30642013
679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG250100.1

Sequence Viewer

Length: 285 bp
ATGGCTTACGGTCAAATTGGTACAGGGAAGATTTACACACTTGGCAGGCTGGGTTGTGATGATGCATCGGAGCGTGGTATTATGGCTAGAGCTTTGCAGGACATTATTTTGAGTACGGCTCCGGAGTCTGATACAGTGGAAGTTTCATACTTGCAGCTGTACATGGAATCTATACAAGATTTGCTTGCACTAGAAAAGACTAATATTCCCATTCACGAGGACCCTAAGGCCAGTGAGGTGTCACTTCCTGGTGCTTCGGTAGTAAGAATCAAAGACCTTCTTTAA

Protein Analysis

94

Amino Acids

10.21

Weight (kDa)

4.53

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 1 - 92 2e-16 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 121
AfaI GTAC 3 cut(s) 22, 115, 161
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 2 cut(s) 92, 157
AluI AGCT 2 cut(s) 92, 157
Aor13HI TCCGGA 1 cut(s) 121
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 220
AvaII GGWCC 1 cut(s) 220
AxyI CCTNAGG 1 cut(s) 225
BauI CACGAG 1 cut(s) 215
BbvI GCAGC 1 cut(s) 166
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 249
BfaI CTAG 2 cut(s) 87, 191
BisI GCNGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 156
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 2 cut(s) 120, 222
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 52, 74
BplI GAGNNNNNCTC 2 cut(s) 103, 135
BsaWI WCCGGW 1 cut(s) 121
Bse1I ACTGG 1 cut(s) 231
Bse21I CCTNAGG 1 cut(s) 225
BseAI TCCGGA 1 cut(s) 121
BseBI CCWGG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 231
BseXI GCAGC 1 cut(s) 166
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 1 cut(s) 230
BsiSI CCGG 1 cut(s) 122
BsnI GGCC 1 cut(s) 230
Bsp13I TCCGGA 1 cut(s) 121
Bsp1407I TGTACA 1 cut(s) 159
BspANI GGCC 1 cut(s) 230
BspEI TCCGGA 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 120, 222
BsrGI TGTACA 1 cut(s) 159
BsrI ACTGG 1 cut(s) 231
BssSI CACGAG 1 cut(s) 215
Bst2BI CACGAG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 11, 136
BstAUI TGTACA 1 cut(s) 159
BstC8I GCNNGC 2 cut(s) 47, 186
BstDEI CTNAG 1 cut(s) 225
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 166
Bsu36I CCTNAGG 1 cut(s) 225
BsuRI GGCC 1 cut(s) 230
BtsIMutI CAGTG 2 cut(s) 141, 238
Cac8I GCNNGC 2 cut(s) 47, 186
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 3 cut(s) 21, 114, 160
CviAII CATG 1 cut(s) 163
CviJI RGCY 7 cut(s) 5, 49, 86, 92, 119, 157, 230
CviKI_1 RGCY 7 cut(s) 5, 49, 86, 92, 119, 157, 230
CviQI GTAC 3 cut(s) 21, 114, 160
DdeI CTNAG 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 220
Eco81I CCTNAGG 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 67
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 83, 148, 164, 173
FalI AAGNNNNNCTT 3 cut(s) 168, 200, 264
FatI CATG 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 155
FspBI CTAG 2 cut(s) 87, 191
GluI GCNGC 1 cut(s) 155
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 122
Hin1II CATG 1 cut(s) 166
HinfI GANTC 3 cut(s) 125, 167, 267
HpaII CCGG 1 cut(s) 122
Hpy188I TCNGA 2 cut(s) 70, 130
Hpy188III TCNNGA 2 cut(s) 122, 215
HpyCH4III ACNGT 2 cut(s) 11, 136
HpyCH4V TGCA 4 cut(s) 65, 97, 154, 188
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 1 cut(s) 166
Kpn2I TCCGGA 1 cut(s) 121
LmnI GCTCC 2 cut(s) 70, 124
LpnPI CCDG 8 cut(s) 9, 31, 35, 83, 135, 234, 244, 261
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 2 cut(s) 52, 74
MaeI CTAG 2 cut(s) 87, 191
MaeIII GTNAC 1 cut(s) 240
MboII GAAGA 1 cut(s) 40
MluCI AATT 1 cut(s) 15
MlyI GAGTC 1 cut(s) 134
MnlI CCTC 2 cut(s) 211, 229
Mph1103I ATGCAT 1 cut(s) 67
MroI TCCGGA 1 cut(s) 121
MseI TTAA 1 cut(s) 283
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 120, 222
NmuCI GTSAC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 67
PfeI GAWTC 2 cut(s) 167, 267
PkrI GCNGC 1 cut(s) 156
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 120, 222
PspPI GGNCC 1 cut(s) 220
PspPPI RGGWCCY 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 3 cut(s) 22, 115, 161
RsaNI GTAC 3 cut(s) 21, 114, 160
SaqAI TTAA 1 cut(s) 283
SatI GCNGC 1 cut(s) 155
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 249
SetI ASST 4 cut(s) 94, 159, 240, 279
SfaNI GCATC 2 cut(s) 52, 74
SinI GGWCC 1 cut(s) 220
Sse9I AATT 1 cut(s) 15
SspI AATATT 1 cut(s) 205
SspMI CTAG 2 cut(s) 87, 191
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 11, 136
TasI AATT 1 cut(s) 15
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 2 cut(s) 167, 267
Tru1I TTAA 1 cut(s) 283
Tru9I TTAA 1 cut(s) 283
TscAI CASTG 2 cut(s) 141, 238
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 1 cut(s) 135
TspRI CASTG 2 cut(s) 141, 238
VpaK11BI GGWCC 1 cut(s) 220
XspI CTAG 2 cut(s) 87, 191
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.