Rh3AG286300

Armadillo repeat-containing kinesin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001912
Physical Location & Seq
Forward (+)
33922840 .. 33935679
12840 bp
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UTR
Exon/CDS
Intron
Rh3AG286300.1

Sequence Viewer

Length: 642 bp
ATGCGCTCCGGCGCTGATGATCGCCGAGGTCCCCATGGTCGCCATCGACTTCATCGAGATCGAAATCAACTCCTCTACCTCGGCCTCATCCCCTTGACTCCGGCCTCGGCCTCATCCACACTGTCGTCCCCGTCGATTTCTCTGGCGACTCTGAGGGATTATCATTGTGAAGTTACGAAGCGGGAATCACTGCCTGCAAGGTTGATCCTAATACTGGCAAGTTGCTCGGCAAACGACGTCGTCTGTTCGCTCCAATCCAGCGTCTTCTTCCACTTCACAGCGCTCCGTCACTCCCACTTCTCGGATTCTGACCTCCAATTCCGAGTTTCAGCAAGATGCTGGAAGAGTGAGTGGTTGCTATTAGAGTTCGGCCAAGAAATGATGAGGATCGAATTTCTGATTTGGATTACGGCGACAAGTGTATTGAATGGAAATAATGGGACAGTCATGGCTTACGGTCAAACTGGTACAGGGAAGATTTACACACTTGGCAGGCTGGGTTGTGATGATGCATTGGAGCGTGGTAATCTGCAACAAATGCATTTGCTTCTGGAACTTTTTAGGTGGCTAGTAAATTCAAACCATAGATATCTTGCATATATGTTGTTAATTGACTATTACCAACTGCTATGCTGTACCTAG

Protein Analysis

213

Amino Acids

24.27

Weight (kDa)

8.51

Isoelectric Point (pI)

46.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 138 - 192 2.9e-08 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
AciI CCGC 1 cut(s) 181
AclWI GGATC 2 cut(s) 199, 395
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 2 cut(s) 392, 574
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 2 cut(s) 469, 637
AfeI AGCGCT 1 cut(s) 282
AfiI CCNNNNNNNGG 2 cut(s) 214, 301
AgsI TTSAA 2 cut(s) 427, 579
AlwI GGATC 2 cut(s) 199, 395
Aor51HI AGCGCT 1 cut(s) 282
AoxI GGCC 4 cut(s) 82, 102, 108, 370
ApoI RAATTY 2 cut(s) 392, 574
AspLEI GCGC 3 cut(s) 6, 14, 283
AspS9I GGNCC 1 cut(s) 29
AvaII GGWCC 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 256
BccI CCATC 1 cut(s) 51
BceAI ACGGC 1 cut(s) 426
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BfaI CTAG 2 cut(s) 569, 640
BfoI RGCGCY 2 cut(s) 15, 284
Bme18I GGWCC 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 29
BmiI GGNNCC 1 cut(s) 31
BmsI GCATC 2 cut(s) 326, 499
BpiI GAAGAC 1 cut(s) 256
BsaBI GATNNNNATC 2 cut(s) 63, 386
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 25, 34, 79, 105
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 301
Bse1I ACTGG 2 cut(s) 219, 469
Bse8I GATNNNNATC 2 cut(s) 63, 386
BseDI CCNNGG 4 cut(s) 25, 34, 79, 105
BseGI GGATG 2 cut(s) 87, 113
BseJI GATNNNNATC 2 cut(s) 63, 386
BseLI CCNNNNNNNGG 2 cut(s) 214, 301
BseMII CTCAG 1 cut(s) 143
BseNI ACTGG 2 cut(s) 219, 469
BseRI GAGGAG 1 cut(s) 62
BseYI CCCAGC 1 cut(s) 496
BshFI GGCC 4 cut(s) 84, 104, 110, 372
BsiSI CCGG 2 cut(s) 9, 101
BslFI GGGAC 3 cut(s) 15, 112, 454
BslI CCNNNNNNNGG 2 cut(s) 214, 301
BsmFI GGGAC 3 cut(s) 15, 112, 454
BsnI GGCC 4 cut(s) 84, 104, 110, 372
Bsp143I GATC 4 cut(s) 19, 58, 204, 387
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 1 cut(s) 181
BspANI GGCC 4 cut(s) 84, 104, 110, 372
BspCNI CTCAG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 2 cut(s) 199, 395
BsrI ACTGG 2 cut(s) 219, 469
BssECI CCNNGG 4 cut(s) 25, 34, 79, 105
BssMI GATC 4 cut(s) 19, 58, 204, 387
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 34
Bst4CI ACNGT 3 cut(s) 123, 445, 458
Bst6I CTCTTC 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 538
BstC8I GCNNGC 2 cut(s) 195, 494
BstDEI CTNAG 1 cut(s) 152
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 2 cut(s) 87, 113
BstH2I RGCGCY 2 cut(s) 15, 284
BstHHI GCGC 3 cut(s) 6, 14, 283
BstKTI GATC 4 cut(s) 22, 61, 207, 390
BstMBI GATC 4 cut(s) 19, 58, 204, 387
BstMWI GCNNNNNNNGC 1 cut(s) 538
BstV2I GAAGAC 1 cut(s) 256
BsuRI GGCC 4 cut(s) 84, 104, 110, 372
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 87, 113
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 119, 188
Cac8I GCNNGC 2 cut(s) 195, 494
CfoI GCGC 3 cut(s) 6, 14, 283
Cfr13I GGNCC 1 cut(s) 29
CseI GACGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 468, 636
CviAII CATG 2 cut(s) 35, 448
CviJI RGCY 7 cut(s) 84, 104, 110, 372, 452, 496, 568
CviKI_1 RGCY 7 cut(s) 84, 104, 110, 372, 452, 496, 568
CviQI GTAC 2 cut(s) 468, 636
DdeI CTNAG 1 cut(s) 152
DpnI GATC 4 cut(s) 21, 60, 206, 389
DpnII GATC 4 cut(s) 19, 58, 204, 387
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco130I CCWWGG 1 cut(s) 34
Eco32I GATATC 1 cut(s) 590
Eco47I GGWCC 1 cut(s) 29
Eco47III AGCGCT 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 29
EcoRV GATATC 1 cut(s) 590
EcoT14I CCWWGG 1 cut(s) 34
EcoT22I ATGCAT 2 cut(s) 514, 543
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 2 cut(s) 38, 451
FaiI YATR 7 cut(s) 36, 449, 585, 598, 600, 602, 631
FaqI GGGAC 3 cut(s) 15, 112, 454
FatI CATG 2 cut(s) 34, 447
FauI CCCGC 1 cut(s) 174
FokI GGATG 2 cut(s) 74, 100
FspBI CTAG 2 cut(s) 569, 640
GlaI GCGC 3 cut(s) 5, 13, 282
GsaI CCCAGC 1 cut(s) 500
HaeII RGCGCY 2 cut(s) 15, 284
HaeIII GGCC 4 cut(s) 84, 104, 110, 372
HapII CCGG 2 cut(s) 9, 101
HgaI GACGC 1 cut(s) 250
HhaI GCGC 3 cut(s) 6, 14, 283
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 2 cut(s) 38, 451
Hin6I GCGC 3 cut(s) 4, 12, 281
HinP1I GCGC 3 cut(s) 4, 12, 281
HinfI GANTC 4 cut(s) 97, 148, 185, 305
HpaII CCGG 2 cut(s) 9, 101
Hpy188I TCNGA 5 cut(s) 153, 304, 310, 323, 399
Hpy188III TCNNGA 2 cut(s) 56, 551
Hpy99I CGWCG 3 cut(s) 136, 239, 242
HpyCH4III ACNGT 3 cut(s) 123, 445, 458
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 5 cut(s) 197, 512, 532, 541, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 538
HpyF3I CTNAG 1 cut(s) 152
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 2 cut(s) 38, 451
HspAI GCGC 3 cut(s) 4, 12, 281
Kzo9I GATC 4 cut(s) 19, 58, 204, 387
LmnI GCTCC 4 cut(s) 11, 255, 288, 517
LweI GCATC 2 cut(s) 326, 499
MaeI CTAG 2 cut(s) 569, 640
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 172, 287
MalI GATC 4 cut(s) 21, 60, 206, 389
MboI GATC 4 cut(s) 19, 58, 204, 387
MboII GAAGA 4 cut(s) 256, 259, 355, 487
MluCI AATT 4 cut(s) 317, 392, 574, 609
MlyI GAGTC 2 cut(s) 91, 142
MnlI CCTC 9 cut(s) 20, 83, 89, 95, 115, 121, 147, 323, 378
Mph1103I ATGCAT 2 cut(s) 514, 543
MseI TTAA 1 cut(s) 608
MspI CCGG 2 cut(s) 9, 101
MwoI GCNNNNNNNGC 1 cut(s) 538
NcoI CCATGG 1 cut(s) 34
NdeII GATC 4 cut(s) 19, 58, 204, 387
NlaIII CATG 2 cut(s) 38, 451
NlaIV GGNNCC 1 cut(s) 31
NmeAIII GCCGAG 4 cut(s) 50, 60, 86, 206
NmuCI GTSAC 1 cut(s) 287
NsiI ATGCAT 2 cut(s) 514, 543
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 131
PfeI GAWTC 2 cut(s) 185, 305
PflFI GACNNNGTC 1 cut(s) 239
PleI GAGTC 2 cut(s) 91, 142
PpsI GAGTC 2 cut(s) 91, 142
PpuMI RGGWCCY 1 cut(s) 29
Psp5II RGGWCCY 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 496
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 29
PspPPI RGGWCCY 1 cut(s) 29
PsyI GACNNNGTC 1 cut(s) 239
RsaI GTAC 2 cut(s) 469, 637
RsaNI GTAC 2 cut(s) 468, 636
SaqAI TTAA 1 cut(s) 608
Sau3AI GATC 4 cut(s) 19, 58, 204, 387
Sau96I GGNCC 1 cut(s) 29
SchI GAGTC 2 cut(s) 91, 142
SetI ASST 7 cut(s) 31, 81, 203, 240, 315, 566, 641
SfaNI GCATC 2 cut(s) 326, 499
SinI GGWCC 1 cut(s) 29
Sse9I AATT 4 cut(s) 317, 392, 574, 609
SsiI CCGC 1 cut(s) 181
SspMI CTAG 2 cut(s) 569, 640
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 3 cut(s) 123, 445, 458
TaiI ACGT 1 cut(s) 240
TaqI TCGA 5 cut(s) 46, 55, 61, 134, 390
TasI AATT 4 cut(s) 317, 392, 574, 609
TfiI GAWTC 2 cut(s) 185, 305
Tru1I TTAA 1 cut(s) 608
Tru9I TTAA 1 cut(s) 608
TscAI CASTG 2 cut(s) 126, 195
TseFI GTSAC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 41
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 2 cut(s) 126, 195
Tth111I GACNNNGTC 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 29
XapI RAATTY 2 cut(s) 392, 574
XspI CTAG 2 cut(s) 569, 640
ZraI GACGTC 1 cut(s) 238
Zsp2I ATGCAT 2 cut(s) 514, 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.