Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0001912

Physical Location & Seq

Genomic Coordinates Reverse (-)

54233169 .. 54241842

Gene Structure 8674 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG308500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGGCTTCTTCGTCGTTGAGATCTTCGTCGACGACAACGGCAGCGCCCGCGGCGAACAATGGCGGAGTCGGAAGGTCCGAGAGGCAGCAACTCCATCCCGCTCGACAAACGACGTCGTCCGTTCGCTCTAAGCCGGCGTCTTCTTCCACTTCACGGCGCTCCGTCACTCCTACTTCTCGGATTCCGACCTCCAATTCCGAGTTTCAGCAAGATGCTGGAAGAGTGAGGGTTGCTGTTAGAGTTCGGCCGAGAAATGATGAGGATCGAATTTCCGATTCGGATTACGGCGACAGTGTTGAGGTCCAGCCAGAGTTGAAGCGGTTAAAGTTGAGGAAAAACAATTGGATGTCCGAGTCTTATAGATTTGATGAAATTTTTACTGAGACTGCCTCGCAAAACCGTGTTTACCAAGTGGTAGCAAAGCCTGTAGTTGAGAGTGTGTTAAACGGATATAATGGGACAGTCATGGCTTACGGTCAAACTGGTACTGGGAAGACTTACACACTTGGTAGGCTGGGTTGTGATGATGCATCGGAGCGTGGTATTATGGTTAGAGCTTTGGAGGACATTATTTTCAGTACCGCTCCAGAGTCTGATACTGTGGAAGTTTCATACTTGCAGCTGTACATGGAATCTATACAAGATTTACTTGCACCAGAAAAGACTAATATTCCCATTCACGAGGACCCTAAGACCGGTGAGGTGTCACTTCCTGGCGCTTCGGTAGTAAGAATCAAAGACCTTGATCATTTCTTACAACTCCTGCAAATTGGTGAGGCAAACCGGCATGCAGCTAACACAAATCTAAACACGGAATCTTCTCGTAGTCATGCCATTCTCATGGTTTTCATTCGTAGATCTGTGGAAGACAAATCTACAGATGAGATCATTTCTCAGGAGAAAGCCTCGAGAAACGGCTTACCTATAATTCGAAGAAGCAAGTTGCTCATTGTTGATCTTGCTGGATCCGAAAGAGTTGATAAATCTGGCAGTGAGGGCCACTTACTCGAGGAGGCTAAATTTATTAATCTTTCACTGACTTCCCTTGGGAAATGTATAAATGCATTGGCTGAAAACAGTCCACATATCCCAACCAGAGATTCCAAGCTTACAAGGTTGCTACGTGATTCATTTGGAGGTACAGCGAGGACATCACTTATAATAACGATTGGACCATCAGCACGACATTATGCAGAGACCAGTAGCACAGTAATGTTTGGACAACGGGCAATGAAAATAGTAAACACAGTAAAACTTAAAGAAGAATTTGATTATGAAAGTCAATGTCGGAAGCTTGAGAACCAAATAGACAATCTCACTGCAGAATTTGAACGGCAACTGAAGTTGAGGGACAGTAAAAAATATGAATTGGAAAGACAGCTGACAGAGTGTCAACACTCTTTTGCTGAGGCCGAAGAAAATCTGGTCACAAGGTCTGAGTTTTTGGAGAAGGAAAATACTCGTTTAGGGTTGGAGATGAAAGATCTTTTATATGAATTGAACAGTCAGAAAGAACACCATGATATGATGCATGATAAGGTGGCACAGCTTGAATCGAGTTTGGAACAGGGCAAGCAACACCAGGTTGAAAGTTCTGCATATCAGAAAATACTAGCTGATACCACTCAGATGTATGAAGAGAAAATAGCAAAGTTGATGAAGCAGCTAGAAGATGAGCATACTCGTTATGGAAGCACAGAGGGGCAGTTAGATGCGATGAGGAAGCTTCTGACTGATGGCCAGAAGACTATTCAGCGACATGAGATGGAACATTCTACATATCAAAAAGCACTTGCTGACACAACTCAGATGTATGAGAGGAAAATAGCCGAGCTAATGGAGCAATTGGAAGATGAGCATTCCCGTTTTGAAGATTTAAAAGAACAAGTTAATTTGGCAAAGAAGCTACGTCATCAACAAAGTTCAATGAAGGGGAAGAAGGAGAGCGATGAACTTAGAGTGAAGTTACAAGACATGAAGCAGCTGCACGAATCAACTCTAAACGAATTTCAGTCCTTGAAATCAGACTATTCTGATCTATCAGAAGAGAAGGCTAGACTAGATGAAGAACTTCAAGATGTGAAGCAAAGACTTCTATTGGAAGAGAAGAAGAGGAAAACTATAAATAATGAGCTGGTCATGCTAAAGAAGGATGTACCAGAGAAAGAAGATGATTTTGTGGACAAGAAATCATATATGAAGGAAAATATATATAAAGGGTCTTCTGCTTTGGGGACTCCAATGGGTTTACACAATTCAAATCCATCAAGGGAGTTGCTATCTAGCCAGAGGGAGACACCTTCTAATCAGAGGGCTACAATTGCAAAAATATGTGAAGAAGTTGGGCTTCAAAAGATTGTACAACTGATGACATCTGATGATTTAGATGTCCAAACCCATGCTGTGAAGATGGTAGCCAATCTTGCAGCTGAAGCATCAAAGGACAACTACAATTCAATCTTACTTGTGATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

824

Amino Acids

93.38

Weight (kDa)

5.94

Isoelectric Point (pI)

46.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	71 - 217	1.5e-18	Microtubule binding
Kinesin	PF00225	80 - 413	7.1e-98	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54870 AT3G54870 AT3G54870
fragaria_vesca	FvH4_2g41290 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790 FvH4_7g18790
malus_domestica	MD01G1103200.v1.1 MD07G1169900.v1.1
prunus_persica	Prupe.2G208800_v2.0.a1 Prupe.2G208800_v2.0.a1 Prupe.2G208800_v2.0.a1
pyrus_communis	pycom01g12970 pycom07g16380
rosa_chinensis	RchiOBHm_Chr1g0362591 RchiOBHm_Chr3g0488101
rosa_laevigata	RLG00000027636
rosa_multiflora	Rmu_co8281713.1_g000001 Rmu_sc0001716.1_g000001
rosa_roxburghii	Rroxscaffold_1G00043680 Rroxscaffold_2G00076710 Rroxscaffold_3G00218650 Rroxscaffold_4G00293920 Rroxscaffold_4G00293930
rosa_rugosa	Rorug01G0299900.1
rosa_samantha	Rh1AG308500 Rh1BG272200 Rh1CG289400 Rh1DG302600 Rh2AG571000 Rh2BG101900 Rh2CG104300 Rh3AG286300 Rh5AG442000 Rh5BG250100 Rh5BG250200 Rh5BG276500
rosa_wichuraiana	Rw1G008860 Rw1G027400 Rw6G016290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1160
AatII	GACGTC	1 cut(s)	116
AccBSI	CCGCTC	2 cut(s)	101, 584
AccI	GTMKAC	1 cut(s)	29
AccII	CGCG	1 cut(s)	50
AciI	CCGC	6 cut(s)	48, 50, 63, 99, 319, 582
AclWI	GGATC	3 cut(s)	270, 960, 973
AcoI	YGGCCR	2 cut(s)	245, 1738
AcsI	RAATTY	6 cut(s)	267, 372, 1019, 1265, 1325, 2006
AcuI	CTGAAG	2 cut(s)	1361, 2451
AcyI	GRCGYC	2 cut(s)	113, 137
AfaI	GTAC	6 cut(s)	487, 580, 626, 1141, 2157, 2361
AfiI	CCNNNNNNNGG	2 cut(s)	153, 695
AgeI	ACCGGT	1 cut(s)	695
AhdI	GACNNNNNGTC	1 cut(s)	1389
AjnI	CCWGG	2 cut(s)	712, 1581
AjuI	GAANNNNNNNTTGG	2 cut(s)	1084, 1116
Alw26I	GTCTC	3 cut(s)	377, 1190, 2288
AlwI	GGATC	3 cut(s)	270, 960, 973
AlwNI	CAGNNNCTG	1 cut(s)	593
Ama87I	CYCGRG	2 cut(s)	907, 1007
AoxI	GGCC	4 cut(s)	245, 997, 1410, 1738
ApeKI	GCWGC	8 cut(s)	41, 85, 619, 791, 1663, 1981, 1984, 2426
ApoI	RAATTY	6 cut(s)	267, 372, 1019, 1265, 1325, 2006
AseI	ATTAAT	1 cut(s)	1026
AsiGI	ACCGGT	1 cut(s)	695
Asp700I	GAANNNNTTC	1 cut(s)	2070
AspLEI	GCGC	3 cut(s)	46, 159, 719
AspS9I	GGNCC	5 cut(s)	75, 301, 685, 997, 1172
AsuHPI	GGTGA	2 cut(s)	710, 785
AsuII	TTCGAA	1 cut(s)	931
AvaI	CYCGRG	2 cut(s)	907, 1007
AvaII	GGWCC	4 cut(s)	75, 301, 685, 1172
BalI	TGGCCA	1 cut(s)	1740
BamHI	GGATCC	1 cut(s)	965
BauI	CACGAG	1 cut(s)	680
BbsI	GAAGAC	5 cut(s)	132, 500, 873, 1751, 2214
BbvCI	CCTCAGC	1 cut(s)	1407
BbvI	GCAGC	8 cut(s)	53, 97, 631, 803, 1675, 1971, 1993, 2438
BccI	CCATC	6 cut(s)	102, 1183, 1730, 1759, 2272, 2404
BceAI	ACGGC	5 cut(s)	54, 170, 301, 931, 1349
BciT130I	CCWGG	2 cut(s)	714, 1583
BclI	TGATCA	1 cut(s)	745
BcoDI	GTCTC	3 cut(s)	377, 1190, 2288
BfaI	CTAG	6 cut(s)	1613, 1667, 2055, 2060, 2283, 2473
BfmI	CTRYAG	3 cut(s)	426, 876, 1320
BfoI	RGCGCY	3 cut(s)	47, 160, 720
BglII	AGATCT	3 cut(s)	20, 857, 1483
BisI	GCNGC	9 cut(s)	42, 51, 86, 620, 792, 1664, 1982, 1985, 2427
BlsI	GCNGC	9 cut(s)	43, 52, 87, 621, 793, 1665, 1983, 1986, 2428
Bme1390I	CCNGG	2 cut(s)	714, 1583
Bme18I	GGWCC	4 cut(s)	75, 301, 685, 1172
BmeRI	GACNNNNNGTC	1 cut(s)	1389
BmeT110I	CYCGRG	2 cut(s)	907, 1007
BmgT120I	GGNCC	5 cut(s)	75, 301, 685, 997, 1172
BmiI	GGNNCC	2 cut(s)	687, 967
BmrFI	CCNGG	2 cut(s)	714, 1583
BmrI	ACTGGG	1 cut(s)	498
BmsI	GCATC	6 cut(s)	202, 517, 539, 1518, 1702, 2444
BmuI	ACTGGG	1 cut(s)	498
BpiI	GAAGAC	5 cut(s)	132, 500, 873, 1751, 2214
BplI	GAGNNNNNCTC	4 cut(s)	374, 406, 890, 922
BpmI	CTGGAG	1 cut(s)	570
Bpu10I	CCTNAGC	1 cut(s)	1407
Bpu14I	TTCGAA	1 cut(s)	931
BpuEI	CTTGAG	1 cut(s)	1316
BsaAI	YACGTR	1 cut(s)	1124
BsaBI	GATNNNNATC	1 cut(s)	261
BsaHI	GRCGYC	2 cut(s)	113, 137
BsaI	GGTCTC	1 cut(s)	1190
BsaJI	CCNNGG	2 cut(s)	48, 1045
BsaWI	WCCGGW	1 cut(s)	695
Bsc4I	CCNNNNNNNGG	2 cut(s)	153, 695
Bse118I	RCCGGY	3 cut(s)	133, 695, 783
Bse1I	ACTGG	3 cut(s)	487, 493, 1200
Bse3DI	GCAATG	1 cut(s)	1236
Bse8I	GATNNNNATC	1 cut(s)	261
BseBI	CCWGG	2 cut(s)	714, 1583
BseDI	CCNNGG	2 cut(s)	48, 1045
BseGI	GGATG	3 cut(s)	94, 351, 2158
BseJI	GATNNNNATC	1 cut(s)	261
BseLI	CCNNNNNNNGG	2 cut(s)	153, 695
BseMI	GCAATG	1 cut(s)	1236
BseMII	CTCAG	6 cut(s)	372, 908, 1398, 1428, 1640, 1820
BseNI	ACTGG	3 cut(s)	487, 493, 1200
BseRI	GAGGAG	1 cut(s)	1025
BseX3I	CGGCCG	1 cut(s)	245
BseXI	GCAGC	8 cut(s)	53, 97, 631, 803, 1675, 1971, 1993, 2438
BseYI	CCCAGC	1 cut(s)	514
BsgI	GTGCAG	1 cut(s)	1970
Bsh1236I	CGCG	1 cut(s)	50
Bsh1285I	CGRYCG	1 cut(s)	248
BshFI	GGCC	4 cut(s)	247, 999, 1412, 1740
BshTI	ACCGGT	1 cut(s)	695
BsiEI	CGRYCG	1 cut(s)	248
BsiHKCI	CYCGRG	2 cut(s)	907, 1007
BsiSI	CCGG	3 cut(s)	134, 696, 784
BslFI	GGGAC	3 cut(s)	472, 1364, 2248
BslI	CCNNNNNNNGG	2 cut(s)	153, 695
BsmAI	GTCTC	3 cut(s)	377, 1190, 2288
BsmFI	GGGAC	3 cut(s)	472, 1364, 2248
BsmI	GAATGC	1 cut(s)	1858
BsnI	GGCC	4 cut(s)	247, 999, 1412, 1740
Bso31I	GGTCTC	1 cut(s)	1190
BsoBI	CYCGRG	2 cut(s)	907, 1007
Bsp119I	TTCGAA	1 cut(s)	931
Bsp1407I	TGTACA	2 cut(s)	624, 2359
BspACI	CCGC	6 cut(s)	48, 50, 63, 99, 319, 582
BspANI	GGCC	4 cut(s)	247, 999, 1412, 1740
BspCNI	CTCAG	6 cut(s)	373, 907, 1399, 1429, 1639, 1819
BspFNI	CGCG	1 cut(s)	50
BspLI	GGNNCC	2 cut(s)	687, 967
BspMAI	CTGCAG	1 cut(s)	1324
BspPI	GGATC	3 cut(s)	270, 960, 973
BspT104I	TTCGAA	1 cut(s)	931
BspTNI	GGTCTC	1 cut(s)	1190
BsrBI	CCGCTC	2 cut(s)	101, 584
BsrDI	GCAATG	1 cut(s)	1236
BsrFI	RCCGGY	3 cut(s)	133, 695, 783
BsrGI	TGTACA	2 cut(s)	624, 2359
BsrI	ACTGG	3 cut(s)	487, 493, 1200
BssAI	RCCGGY	3 cut(s)	133, 695, 783
BssECI	CCNNGG	2 cut(s)	48, 1045
BssNI	GRCGYC	2 cut(s)	113, 137
BssSI	CACGAG	1 cut(s)	680
BssT1I	CCWWGG	1 cut(s)	1045
Bst2BI	CACGAG	1 cut(s)	680
Bst2UI	CCWGG	2 cut(s)	714, 1583
Bst6I	CTCTTC	5 cut(s)	214, 1632, 2040, 2097, 2105
BstACI	GRCGYC	2 cut(s)	113, 137
BstAUI	TGTACA	2 cut(s)	624, 2359
BstBAI	YACGTR	1 cut(s)	1124
BstBI	TTCGAA	1 cut(s)	931
BstC8I	GCNNGC	4 cut(s)	48, 135, 789, 1574
BstDEI	CTNAG	9 cut(s)	129, 381, 690, 894, 1407, 1437, 1626, 1806, 1955
BstDSI	CCRYGG	1 cut(s)	48
BstF5I	GGATG	3 cut(s)	94, 351, 2158
BstFNI	CGCG	1 cut(s)	50
BstH2I	RGCGCY	3 cut(s)	47, 160, 720
BstHHI	GCGC	3 cut(s)	46, 159, 719
BstMAI	GTCTC	3 cut(s)	377, 1190, 2288
BstMCI	CGRYCG	1 cut(s)	248
BstMWI	GCNNNNNNNGC	8 cut(s)	47, 50, 996, 1840, 2140, 2321, 2423, 2432
BstNI	CCWGG	2 cut(s)	714, 1583
BstNSI	RCATGY	1 cut(s)	791
BstSCI	CCNGG	2 cut(s)	712, 1581
BstSFI	CTRYAG	3 cut(s)	426, 876, 1320
BstUI	CGCG	1 cut(s)	50
BstV1I	GCAGC	8 cut(s)	53, 97, 631, 803, 1675, 1971, 1993, 2438
BstV2I	GAAGAC	5 cut(s)	132, 500, 873, 1751, 2214
BstX2I	RGATCY	4 cut(s)	20, 857, 965, 1483
BstXI	CCANNNNNNTGG	1 cut(s)	841
BstYI	RGATCY	4 cut(s)	20, 857, 965, 1483
BstZI	CGGCCG	1 cut(s)	245
BsuRI	GGCC	4 cut(s)	247, 999, 1412, 1740
BtgI	CCRYGG	1 cut(s)	48
BtgZI	GCGATG	2 cut(s)	1730, 1962
BtsCI	GGATG	3 cut(s)	94, 351, 2158
BtsI	GCAGTG	2 cut(s)	997, 1317
BtsIMutI	CAGTG	4 cut(s)	298, 997, 1034, 1317
Cac8I	GCNNGC	4 cut(s)	48, 135, 789, 1574
CaiI	CAGNNNCTG	1 cut(s)	593
CfoI	GCGC	3 cut(s)	46, 159, 719
Cfr10I	RCCGGY	3 cut(s)	133, 695, 783
Cfr13I	GGNCC	5 cut(s)	75, 301, 685, 997, 1172
Cfr42I	CCGCGG	1 cut(s)	51
CseI	GACGC	1 cut(s)	126
CsiI	ACCWGGT	1 cut(s)	1581
Csp6I	GTAC	6 cut(s)	486, 579, 625, 1140, 2156, 2360
CspAI	ACCGGT	1 cut(s)	695
CviQI	GTAC	6 cut(s)	486, 579, 625, 1140, 2156, 2360
DdeI	CTNAG	9 cut(s)	129, 381, 690, 894, 1407, 1437, 1626, 1806, 1955
DraI	TTTAAA	1 cut(s)	1878
DriI	GACNNNNNGTC	1 cut(s)	1389
EaeI	YGGCCR	2 cut(s)	245, 1738
EagI	CGGCCG	1 cut(s)	245
Eam1104I	CTCTTC	5 cut(s)	214, 1632, 2040, 2097, 2105
Eam1105I	GACNNNNNGTC	1 cut(s)	1389
EarI	CTCTTC	5 cut(s)	214, 1632, 2040, 2097, 2105
EciI	GGCGGA	1 cut(s)	78
EclXI	CGGCCG	1 cut(s)	245
Eco130I	CCWWGG	1 cut(s)	1045
Eco31I	GGTCTC	1 cut(s)	1190
Eco47I	GGWCC	4 cut(s)	75, 301, 685, 1172
Eco52I	CGGCCG	1 cut(s)	245
Eco57I	CTGAAG	2 cut(s)	1361, 2451
Eco88I	CYCGRG	2 cut(s)	907, 1007
EcoO109I	RGGNCCY	1 cut(s)	685
EcoRII	CCWGG	2 cut(s)	712, 1581
EcoT14I	CCWWGG	1 cut(s)	1045
EcoT22I	ATGCAT	3 cut(s)	532, 1066, 1533
ErhI	CCWWGG	1 cut(s)	1045
FalI	AAGNNNNNCTT	4 cut(s)	633, 665, 2331, 2363
FaqI	GGGAC	3 cut(s)	472, 1364, 2248
FauI	CCCGC	2 cut(s)	55, 106
FbaI	TGATCA	1 cut(s)	745
FblI	GTMKAC	1 cut(s)	29
Fnu4HI	GCNGC	9 cut(s)	42, 51, 86, 620, 792, 1664, 1982, 1985, 2427
FokI	GGATG	3 cut(s)	81, 358, 2165
Fsp4HI	GCNGC	9 cut(s)	42, 51, 86, 620, 792, 1664, 1982, 1985, 2427
FspBI	CTAG	6 cut(s)	1613, 1667, 2055, 2060, 2283, 2473
GlaI	GCGC	3 cut(s)	45, 158, 718
GluI	GCNGC	9 cut(s)	42, 51, 86, 620, 792, 1664, 1982, 1985, 2427
GsaI	CCCAGC	1 cut(s)	518
GsuI	CTGGAG	1 cut(s)	570
HaeII	RGCGCY	3 cut(s)	47, 160, 720
HaeIII	GGCC	4 cut(s)	247, 999, 1412, 1740
HapII	CCGG	3 cut(s)	134, 696, 784
HgaI	GACGC	1 cut(s)	126
HhaI	GCGC	3 cut(s)	46, 159, 719
Hin1I	GRCGYC	2 cut(s)	113, 137
Hin6I	GCGC	3 cut(s)	44, 157, 717
HinP1I	GCGC	3 cut(s)	44, 157, 717
HincII	GTYRAC	2 cut(s)	30, 1394
HindII	GTYRAC	2 cut(s)	30, 1394
HindIII	AAGCTT	3 cut(s)	1106, 1292, 1724
HpaII	CCGG	3 cut(s)	134, 696, 784
HphI	GGTGA	2 cut(s)	710, 785
Hpy166II	GTNNAC	7 cut(s)	30, 406, 1082, 1243, 1394, 2182, 2249
Hpy188III	TCNNGA	5 cut(s)	587, 680, 896, 909, 2075
Hpy8I	GTNNAC	7 cut(s)	30, 406, 1082, 1243, 1394, 2182, 2249
Hpy99I	CGWCG	5 cut(s)	16, 31, 34, 115, 118
HpyAV	CCTTC	8 cut(s)	66, 1444, 1924, 1933, 2044, 2143, 2194, 2310
HpyCH4IV	ACGT	3 cut(s)	113, 1123, 1909
HpyF10VI	GCNNNNNNNGC	8 cut(s)	47, 50, 996, 1840, 2140, 2321, 2423, 2432
HpyF3I	CTNAG	9 cut(s)	129, 381, 690, 894, 1407, 1437, 1626, 1806, 1955
HpySE526I	ACGT	3 cut(s)	113, 1123, 1909
Hsp92I	GRCGYC	2 cut(s)	113, 137
HspAI	GCGC	3 cut(s)	44, 157, 717
KroI	GCCGGC	1 cut(s)	133
KroNI	GCCGGC	1 cut(s)	135
Ksp22I	TGATCA	1 cut(s)	745
KspI	CCGCGG	1 cut(s)	51
LmnI	GCTCC	4 cut(s)	164, 535, 589, 1840
Lsp1109I	GCAGC	8 cut(s)	53, 97, 631, 803, 1675, 1971, 1993, 2438
LweI	GCATC	6 cut(s)	202, 517, 539, 1518, 1702, 2444
MabI	ACCWGGT	1 cut(s)	1581
MaeI	CTAG	6 cut(s)	1613, 1667, 2055, 2060, 2283, 2473
MaeII	ACGT	3 cut(s)	113, 1123, 1909
MaeIII	GTNAC	4 cut(s)	163, 705, 1426, 1965
MbiI	CCGCTC	2 cut(s)	101, 584
MfeI	CAATTG	3 cut(s)	340, 1844, 2319
MflI	RGATCY	4 cut(s)	20, 857, 965, 1483
MlsI	TGGCCA	1 cut(s)	1740
MluNI	TGGCCA	1 cut(s)	1740
MlyI	GAGTC	4 cut(s)	75, 362, 599, 2230
MmeI	TCCRAC	4 cut(s)	49, 209, 1268, 1452
Mox20I	TGGCCA	1 cut(s)	1740
Mph1103I	ATGCAT	3 cut(s)	532, 1066, 1533
MroNI	GCCGGC	1 cut(s)	133
MroXI	GAANNNNTTC	1 cut(s)	2070
MscI	TGGCCA	1 cut(s)	1740
MseI	TTAA	6 cut(s)	323, 443, 1026, 1257, 1877, 1890
MslI	CAYNNNNRTG	3 cut(s)	839, 1211, 1628
Msp20I	TGGCCA	1 cut(s)	1740
MspA1I	CMGCKG	5 cut(s)	50, 622, 1381, 1984, 2429
MspI	CCGG	3 cut(s)	134, 696, 784
MspR9I	CCNGG	2 cut(s)	714, 1583
MunI	CAATTG	3 cut(s)	340, 1844, 2319
Mva1269I	GAATGC	1 cut(s)	1858
MvaI	CCWGG	2 cut(s)	714, 1583
MvnI	CGCG	1 cut(s)	50
MwoI	GCNNNNNNNGC	8 cut(s)	47, 50, 996, 1840, 2140, 2321, 2423, 2432
NaeI	GCCGGC	1 cut(s)	135
NgoMIV	GCCGGC	1 cut(s)	133
NlaIV	GGNNCC	2 cut(s)	687, 967
NmeAIII	GCCGAG	2 cut(s)	273, 1855
NmuCI	GTSAC	3 cut(s)	163, 705, 1426
NsiI	ATGCAT	3 cut(s)	532, 1066, 1533
NspI	RCATGY	1 cut(s)	791
NspV	TTCGAA	1 cut(s)	931
PaeI	GCATGC	1 cut(s)	791
PaeR7I	CTCGAG	2 cut(s)	907, 1007
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1858
PdiI	GCCGGC	1 cut(s)	135
PdmI	GAANNNNTTC	1 cut(s)	2070
PfeI	GAWTC	9 cut(s)	181, 275, 632, 732, 815, 1100, 1127, 1553, 1991
PflFI	GACNNNGTC	1 cut(s)	115
PinAI	ACCGGT	1 cut(s)	695
PkrI	GCNGC	9 cut(s)	43, 52, 87, 621, 793, 1665, 1983, 1986, 2428
PleI	GAGTC	4 cut(s)	74, 361, 598, 2230
PpsI	GAGTC	4 cut(s)	74, 361, 598, 2230
Ppu21I	YACGTR	1 cut(s)	1124
PpuMI	RGGWCCY	1 cut(s)	685
PshBI	ATTAAT	1 cut(s)	1026
PsiI	TTATAA	1 cut(s)	1160
Psp5II	RGGWCCY	1 cut(s)	685
Psp6I	CCWGG	2 cut(s)	712, 1581
PspFI	CCCAGC	1 cut(s)	514
PspGI	CCWGG	2 cut(s)	712, 1581
PspN4I	GGNNCC	2 cut(s)	687, 967
PspPI	GGNCC	5 cut(s)	75, 301, 685, 997, 1172
PspPPI	RGGWCCY	1 cut(s)	685
PspXI	VCTCGAGB	1 cut(s)	1007
PstI	CTGCAG	1 cut(s)	1324
PstNI	CAGNNNCTG	1 cut(s)	593
PsuI	RGATCY	4 cut(s)	20, 857, 965, 1483
PsyI	GACNNNGTC	1 cut(s)	115
PvuII	CAGCTG	4 cut(s)	622, 1381, 1984, 2429
RsaI	GTAC	6 cut(s)	487, 580, 626, 1141, 2157, 2361
RsaNI	GTAC	6 cut(s)	486, 579, 625, 1140, 2156, 2360
RseI	CAYNNNNRTG	3 cut(s)	839, 1211, 1628
SacII	CCGCGG	1 cut(s)	51
SalI	GTCGAC	1 cut(s)	28
SaqAI	TTAA	6 cut(s)	323, 443, 1026, 1257, 1877, 1890
SatI	GCNGC	9 cut(s)	42, 51, 86, 620, 792, 1664, 1982, 1985, 2427
Sau96I	GGNCC	5 cut(s)	75, 301, 685, 997, 1172
SchI	GAGTC	4 cut(s)	75, 362, 599, 2230
ScrFI	CCNGG	2 cut(s)	714, 1583
SexAI	ACCWGGT	1 cut(s)	1581
SfaNI	GCATC	6 cut(s)	202, 517, 539, 1518, 1702, 2444
SfcI	CTRYAG	3 cut(s)	426, 876, 1320
Sfr274I	CTCGAG	2 cut(s)	907, 1007
Sfr303I	CCGCGG	1 cut(s)	51
SfuI	TTCGAA	1 cut(s)	931
SgrBI	CCGCGG	1 cut(s)	51
SgrDI	CGTCGACG	1 cut(s)	28
SinI	GGWCC	4 cut(s)	75, 301, 685, 1172
SlaI	CTCGAG	2 cut(s)	907, 1007
SmiMI	CAYNNNNRTG	3 cut(s)	839, 1211, 1628
SmlI	CTYRAG	3 cut(s)	907, 1007, 1295
SmoI	CTYRAG	3 cut(s)	907, 1007, 1295
SphI	GCATGC	1 cut(s)	791
SsiI	CCGC	6 cut(s)	48, 50, 63, 99, 319, 582
SspI	AATATT	1 cut(s)	670
SspMI	CTAG	6 cut(s)	1613, 1667, 2055, 2060, 2283, 2473
StyD4I	CCNGG	2 cut(s)	712, 1581
StyI	CCWWGG	1 cut(s)	1045
TaiI	ACGT	3 cut(s)	116, 1126, 1912
TaqI	TCGA	7 cut(s)	29, 103, 265, 908, 931, 1008, 1556
TatI	WGTACW	2 cut(s)	624, 2359
TauI	GCSGC	1 cut(s)	53
TfiI	GAWTC	9 cut(s)	181, 275, 632, 732, 815, 1100, 1127, 1553, 1991
Tru1I	TTAA	6 cut(s)	323, 443, 1026, 1257, 1877, 1890
Tru9I	TTAA	6 cut(s)	323, 443, 1026, 1257, 1877, 1890
TscAI	CASTG	4 cut(s)	298, 997, 1041, 1324
TseFI	GTSAC	3 cut(s)	163, 705, 1426
TseI	GCWGC	8 cut(s)	41, 85, 619, 791, 1663, 1981, 1984, 2426
Tsp45I	GTSAC	3 cut(s)	163, 705, 1426
TspGWI	ACGGA	4 cut(s)	109, 151, 462, 827
TspRI	CASTG	4 cut(s)	298, 997, 1041, 1324
Tth111I	GACNNNGTC	1 cut(s)	115
VpaK11BI	GGWCC	4 cut(s)	75, 301, 685, 1172
VspI	ATTAAT	1 cut(s)	1026
XapI	RAATTY	6 cut(s)	267, 372, 1019, 1265, 1325, 2006
XceI	RCATGY	1 cut(s)	791
XhoI	CTCGAG	2 cut(s)	907, 1007
XmiI	GTMKAC	1 cut(s)	29
XmnI	GAANNNNTTC	1 cut(s)	2070
XspI	CTAG	6 cut(s)	1613, 1667, 2055, 2060, 2283, 2473
ZraI	GACGTC	1 cut(s)	114
Zsp2I	ATGCAT	3 cut(s)	532, 1066, 1533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.