Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001385

Physical Location & Seq

Genomic Coordinates Reverse (-)

24729258 .. 24731252

Gene Structure 1995 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1137600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGGCATTCCAAAGATTCATACTTTGCTGGGTTTTCCTCCTCTTTCTCCCAAACCCAGCAAAAACCCAGCTCGACCAAGAGCTCTACTTCAACGGCTTCAATGGTGTCGCCAACAGCAGCAACATGAGCTTAAACGGCGTCGCATCAATCGAGCCCAACGGCATGCTAAAGCTCACAAACGACACTCTCAGAGTCCTCGGCCATGCCTTCTACTCCGCCCCAGTTCGATTCAAAAACTCCACCGACGGCAAAGCATTTTCCTTCTCCACCTCCTTCGTCTTCACCATCGTCCCTGAGTACCCAAAGCTCGGCGGCCACGGCCTTGCCTTCATAATCTCGCCGTCGAAATCGCTCCCGGGATCCCTCCCCAGCCAGTACCTCGGCATCCTCAACTCCACCGTCGTCGGCAACTTCTCCAACCACATCTTCGCCGTCGAATTCGACACCGTCCAGAACTTCGAATTCGGGGACATCAACGACAACCACGTCGGAATCGATATCAACAGCCTGGCCTCCAACAAATCGACCCCGGCCGGGTTTTTCACCGGTGGAAATTCGACGAAAGAGGACCTCAATCTCAAGAGTGGGCTCCCGATCCAGGCCTGGGTCGATTACGATTCGGTGAAAAATCAGGTCACTGTCATGCTTTCACCCGATTCGATCAAACCCAGATCCCCGATTTTAACATTCGATGTCGATCTTTCTCCAATTTTGGAAGATTTTATGTACGTTGGGTTCTCTGCTTCGACCGGGTTGCTCGCCAGCTCCCACTACGTCTCCGGTTGGAGCTTTAAGATGAACGGAGAAGCCAAGTCTCTGAATTTAGAAGCTTCGCCTAAACTTCCCGGCCCGAAAGAGAAGCAATCATCAGCGTTGATCATTGGCGTTTCTGTTTCCGTTCTTGTTTTTTTTATCTTGGCCGTCGGATTGGGGTTCTACATTGTTCACAGGATCAAGAACGCTGAGGTGATCGAGCCGTGGGAGCTCGACATTGGGCCCCATATATTCAGATACAGCGAGCTCAAGCAGGCCACCAGAGGGTTCAGGGATGAAGAGGTGATCGGGTTTGGTGGATCTGGTAAGGTTTACAAAGGGACATTGCCGAATTCAGAAACCCAAGTGGCGGTTAAGCGAATTTCGAACGAGGCTAGGCAGGGTCTGCAGGAATTCGTGTCGGAAATTGCCACCATTGGCCGTCTCAGGCACAGGAATTTAGTGCAGTTGTTGGGGTGGTGTCGCCGGCGTGGAGATTTGTTGCTTGTTTATGATTTTATGCCTAATGGAAGCTTGGATAAGTACTTATTTGAGAAGCCGAAAGCAATTTTGAGCTGGGAACAGAGGTTCAAGATAGCTCAAAATGTAGCTTCTGGCTTATTGTATTTGCACGAAGGTTGGGAGCAGACTGTGATTCATAGGGACATAAAAGCAGGAAACGTGCTTTTGGATTCTGAGATGAACGGAAGGCTTGGAGATTTCGGTCTTGCTAAATTGTATGAGCATGGAGCAAACCCTACAACAACTAGGGTTGTTGGGACACTGGGTTACCTAGCTCCCGAGTTGACTCGGACCGGAAAACCGACTCCGAGCTCGGATGTGTTTGCTCTTGGTGCTCTGCTGCTGGAGCTGGTGTGTGGGAGGAGACCCATTGAACGAAAAGCCCTGCCGGAGGAGCTGATATTGGTTGATTGGGTTTGGGAGAAGTGGAAGGCTGGTGCAATTCTTGAAGTTGTGGACCCCAGATTGGAGGATGAGTTTGATGATCTGGAGGCTGTGGTGGTGCTGAAGCTAGGGCTGATGTGCTCAAATAATTCCCCCAGGGCAAGGCCCACAGTGAGGCAGGTGGTGAGATACTTGGAGGGTGAGGCGGCGCTGCCCGACGCGGAGGCGTCTCCCGGGTCTTATAATGGGAAGAAGGGTGGTAGCGATGGCGGTGGAACGGGTGGTGAGTTTGTGGTTTATGTGCATTCCTACGAAGATACAGATACGGCAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0042742 GO:0043207 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase

Protein Analysis

665

Amino Acids

72.97

Weight (kDa)

5.76

Isoelectric Point (pI)

34.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	28 - 278	5.4e-80	Legume lectin domain
Lectin_L-type_dom	PF18483	39 - 257	1.7e-06	Legume lectin beta-barrel domain
Pkinase	PF00069	349 - 554	2e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	351 - 618	2.9e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55550
fragaria_vesca	FvH4_6g13180 FvH4_7g22900
malus_domestica	MD01G1137300.v1.1 MD01G1137400.v1.1 MD01G1137500.v1.1 MD01G1137600.v1.1 MD01G1137700.v1.1 MD04G1133700.v1.1 MD04G1133800.v1.1 MD12G1147700.v1.1
prunus_persica	Prupe.2G238700_v2.0.a1
pyrus_communis	pycom01g15830 pycom01g15860 pycom01g15870 pycom01g15880 pycom04g12170 pycom12g14140
rosa_chinensis	RchiOBHm_Chr1g0368061 RchiOBHm_Chr3g0465661 RchiOBHm_Chr3g0465671 RchiOBHm_Chr3g0465791 RchiOBHm_Chr4g0404141
rosa_laevigata	RLG00000024618 RLG00000024629 RLG00000024630 RLG00000027242
rosa_multiflora	Rmu_sc0000550.1_g000019 Rmu_sc0005103.1_g000004 Rmu_sc0007705.1_g000006 Rmu_sc0007705.1_g000016 Rmu_sc0007705.1_g000021 Rmu_sc0013273.1_g000002
rosa_roxburghii	Rroxscaffold_4G00288890 Rroxscaffold_6G00414940
rosa_rugosa	Rorug01G0338700 Rorug03G0078100 Rorug03G0078200
rosa_samantha	Rh1AG345700 Rh1BG308000 Rh1CG323600 Rh1DG340100 Rh3BG143600 Rh3CG144600 Rh3CG144700 Rh3CG144800 Rh3CG145700 Rh3DG144900 Rh4AG123100 Rh4BG116200
rosa_wichuraiana	Rw1G030770 Rw3G011660 Rw3G011700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1902
AarI	CACCTGC	1 cut(s)	1828
AasI	GACNNNNNNGTC	1 cut(s)	485
Acc36I	ACCTGC	1 cut(s)	1828
AccII	CGCG	1 cut(s)	1880
AciI	CCGC	6 cut(s)	216, 312, 1124, 1865, 1880, 1929
AclWI	GGATC	6 cut(s)	354, 367, 589, 666, 959, 1081
AcoI	YGGCCR	5 cut(s)	199, 313, 531, 918, 1192
AcsI	RAATTY	8 cut(s)	437, 461, 553, 820, 1105, 1134, 1166, 1210
AcuI	CTGAAG	1 cut(s)	1802
AcyI	GRCGYC	2 cut(s)	138, 1886
AfaI	GTAC	4 cut(s)	299, 377, 728, 1298
AgeI	ACCGGT	1 cut(s)	545
AgsI	TTSAA	6 cut(s)	91, 100, 232, 1345, 1649, 1724
AjiI	CACGTC	1 cut(s)	487
AjnI	CCWGG	4 cut(s)	507, 597, 602, 1814
AjuI	GAANNNNNNNTTGG	2 cut(s)	410, 442
AloI	GAACNNNNNNTCC	2 cut(s)	1325, 1357
Alw21I	GWGCWC	6 cut(s)	84, 987, 1023, 1589, 1612, 1802
Alw26I	GTCTC	5 cut(s)	781, 819, 1202, 1633, 1893
AlwI	GGATC	6 cut(s)	354, 367, 589, 666, 959, 1081
AlwNI	CAGNNNCTG	1 cut(s)	1159
Ama87I	CYCGRG	3 cut(s)	355, 1553, 1892
ApaI	GGGCCC	1 cut(s)	999
ApeKI	GCWGC	3 cut(s)	117, 1615, 1870
ApoI	RAATTY	8 cut(s)	437, 461, 553, 820, 1105, 1134, 1166, 1210
AsiGI	ACCGGT	1 cut(s)	545
AspLEI	GCGC	1 cut(s)	1870
AspS9I	GGNCC	7 cut(s)	568, 848, 995, 996, 1566, 1732, 1824
AsuC2I	CCSGG	8 cut(s)	356, 357, 530, 535, 751, 846, 1893, 1894
AsuHPI	GGTGA	9 cut(s)	274, 535, 634, 642, 979, 1069, 1855, 1871, 1955
AsuII	TTCGAA	2 cut(s)	459, 1139
AvaI	CYCGRG	3 cut(s)	355, 1553, 1892
AvaII	GGWCC	3 cut(s)	568, 1566, 1732
BaeGI	GKGCMC	1 cut(s)	999
BamHI	GGATCC	1 cut(s)	359
BanII	GRGCYC	7 cut(s)	84, 156, 591, 987, 999, 1023, 1589
BbsI	GAAGAC	1 cut(s)	271
Bbv12I	GWGCWC	6 cut(s)	84, 987, 1023, 1589, 1612, 1802
BbvCI	CCTCAGC	1 cut(s)	963
BbvI	GCAGC	3 cut(s)	129, 1602, 1857
BccI	CCATC	2 cut(s)	293, 1919
BcgI	CGANNNNNNTGC	2 cut(s)	736, 770
BciT130I	CCWGG	4 cut(s)	509, 599, 604, 1816
BclI	TGATCA	1 cut(s)	876
BcnI	CCSGG	8 cut(s)	356, 357, 530, 535, 751, 846, 1893, 1894
BcoDI	GTCTC	5 cut(s)	781, 819, 1202, 1633, 1893
BfaI	CTAG	4 cut(s)	1149, 1521, 1547, 1787
BfmI	CTRYAG	1 cut(s)	1160
BfoI	RGCGCY	1 cut(s)	1871
BfuAI	ACCTGC	1 cut(s)	1828
BisI	GCNGC	5 cut(s)	118, 313, 1616, 1866, 1871
BlsI	GCNGC	5 cut(s)	119, 314, 1617, 1867, 1872
BmcAI	AGTACT	1 cut(s)	1298
Bme18I	GGWCC	3 cut(s)	568, 1566, 1732
BmeT110I	CYCGRG	3 cut(s)	355, 1553, 1892
BmgBI	CACGTC	1 cut(s)	487
BmgT120I	GGNCC	7 cut(s)	568, 848, 995, 996, 1566, 1732, 1824
BmiI	GGNNCC	5 cut(s)	361, 590, 997, 998, 1734
BmrI	ACTGGG	2 cut(s)	215, 1547
BmsI	GCATC	2 cut(s)	152, 393
BmuI	ACTGGG	2 cut(s)	215, 1547
BpiI	GAAGAC	1 cut(s)	271
BpmI	CTGGAG	2 cut(s)	1640, 1784
Bpu10I	CCTNAGC	1 cut(s)	963
Bpu14I	TTCGAA	2 cut(s)	459, 1139
BpuEI	CTTGAG	2 cut(s)	563, 1007
BpuMI	CCSGG	8 cut(s)	356, 357, 530, 535, 751, 846, 1893, 1894
Bsa29I	ATCGAT	1 cut(s)	495
BsaBI	GATNNNNATC	1 cut(s)	696
BsaHI	GRCGYC	2 cut(s)	138, 1886
BsaI	GGTCTC	1 cut(s)	1633
BsaWI	WCCGGW	3 cut(s)	545, 779, 1568
BsaXI	ACNNNNNCTCC	6 cut(s)	761, 791, 1613, 1643, 1736, 1766
Bse118I	RCCGGY	2 cut(s)	545, 1239
Bse1I	ACTGG	3 cut(s)	221, 373, 1542
Bse3DI	GCAATG	1 cut(s)	1097
Bse8I	GATNNNNATC	1 cut(s)	696
BseBI	CCWGG	4 cut(s)	509, 599, 604, 1816
BseCI	ATCGAT	1 cut(s)	495
BseGI	GGATG	4 cut(s)	384, 1054, 1597, 1753
BseJI	GATNNNNATC	1 cut(s)	696
BseMI	GCAATG	1 cut(s)	1097
BseMII	CTCAG	5 cut(s)	202, 285, 954, 1213, 1440
BseNI	ACTGG	3 cut(s)	221, 373, 1542
BseRI	GAGGAG	3 cut(s)	29, 1651, 1682
BseSI	GKGCMC	1 cut(s)	999
BseX3I	CGGCCG	1 cut(s)	531
BseXI	GCAGC	3 cut(s)	129, 1602, 1857
BseYI	CCCAGC	5 cut(s)	27, 55, 66, 368, 1329
BsgI	GTGCAG	1 cut(s)	1238
Bsh1236I	CGCG	1 cut(s)	1880
Bsh1285I	CGRYCG	2 cut(s)	534, 750
BshTI	ACCGGT	1 cut(s)	545
BshVI	ATCGAT	1 cut(s)	495
BsiEI	CGRYCG	2 cut(s)	534, 750
BsiHKAI	GWGCWC	6 cut(s)	84, 987, 1023, 1589, 1612, 1802
BsiHKCI	CYCGRG	3 cut(s)	355, 1553, 1892
BslFI	GGGAC	5 cut(s)	275, 482, 1108, 1430, 1546
BsmAI	GTCTC	5 cut(s)	781, 819, 1202, 1633, 1893
BsmBI	CGTCTC	3 cut(s)	781, 1202, 1893
BsmFI	GGGAC	5 cut(s)	275, 482, 1108, 1430, 1546
BsmI	GAATGC	2 cut(s)	5, 1963
Bso31I	GGTCTC	1 cut(s)	1633
BsoBI	CYCGRG	3 cut(s)	355, 1553, 1892
Bsp119I	TTCGAA	2 cut(s)	459, 1139
Bsp120I	GGGCCC	1 cut(s)	995
Bsp1286I	GDGCHC	9 cut(s)	84, 156, 591, 987, 999, 1023, 1589, 1612, 1802
BspACI	CCGC	6 cut(s)	216, 312, 1124, 1865, 1880, 1929
BspCNI	CTCAG	5 cut(s)	201, 286, 955, 1212, 1441
BspDI	ATCGAT	1 cut(s)	495
BspFNI	CGCG	1 cut(s)	1880
BspLI	GGNNCC	5 cut(s)	361, 590, 997, 998, 1734
BspMAI	CTGCAG	1 cut(s)	1164
BspMI	ACCTGC	1 cut(s)	1828
BspPI	GGATC	6 cut(s)	354, 367, 589, 666, 959, 1081
BspT104I	TTCGAA	2 cut(s)	459, 1139
BspTNI	GGTCTC	1 cut(s)	1633
BsrDI	GCAATG	1 cut(s)	1097
BsrFI	RCCGGY	2 cut(s)	545, 1239
BsrI	ACTGG	3 cut(s)	221, 373, 1542
BssAI	RCCGGY	2 cut(s)	545, 1239
BssNI	GRCGYC	2 cut(s)	138, 1886
Bst2UI	CCWGG	4 cut(s)	509, 599, 604, 1816
Bst4CI	ACNGT	5 cut(s)	400, 448, 640, 1405, 1831
Bst6I	CTCTTC	1 cut(s)	1047
BstACI	GRCGYC	2 cut(s)	138, 1886
BstAPI	GCANNNNNTGC	1 cut(s)	1159
BstBI	TTCGAA	2 cut(s)	459, 1139
BstC8I	GCNNGC	6 cut(s)	164, 759, 763, 1019, 1029, 1241
BstDEI	CTNAG	5 cut(s)	188, 294, 963, 1199, 1449
BstDSI	CCRYGG	2 cut(s)	316, 977
BstEII	GGTNACC	1 cut(s)	1541
BstENI	CCTNNNNNAGG	1 cut(s)	1664
BstF5I	GGATG	4 cut(s)	384, 1054, 1597, 1753
BstFNI	CGCG	1 cut(s)	1880
BstH2I	RGCGCY	1 cut(s)	1871
BstHHI	GCGC	1 cut(s)	1870
BstMAI	GTCTC	5 cut(s)	781, 819, 1202, 1633, 1893
BstMCI	CGRYCG	2 cut(s)	534, 750
BstMWI	GCNNNNNNNGC	8 cut(s)	126, 135, 318, 982, 1159, 1607, 1621, 1669
BstNI	CCWGG	4 cut(s)	509, 599, 604, 1816
BstNSI	RCATGY	1 cut(s)	166
BstPI	GGTNACC	1 cut(s)	1541
BstSFI	CTRYAG	1 cut(s)	1160
BstSLI	GKGCMC	1 cut(s)	999
BstUI	CGCG	1 cut(s)	1880
BstV1I	GCAGC	3 cut(s)	129, 1602, 1857
BstV2I	GAAGAC	1 cut(s)	271
BstX2I	RGATCY	3 cut(s)	359, 671, 1073
BstYI	RGATCY	3 cut(s)	359, 671, 1073
BstZI	CGGCCG	1 cut(s)	531
Bsu15I	ATCGAT	1 cut(s)	495
BsuTUI	ATCGAT	1 cut(s)	495
BtgI	CCRYGG	2 cut(s)	316, 977
BtgZI	GCGATG	1 cut(s)	1938
BtrI	CACGTC	1 cut(s)	487
BtsCI	GGATG	4 cut(s)	384, 1054, 1597, 1753
BtsIMutI	CAGTG	3 cut(s)	636, 1535, 1836
BveI	ACCTGC	1 cut(s)	1828
Cac8I	GCNNGC	6 cut(s)	164, 759, 763, 1019, 1029, 1241
CaiI	CAGNNNCTG	1 cut(s)	1159
CfoI	GCGC	1 cut(s)	1870
Cfr10I	RCCGGY	2 cut(s)	545, 1239
Cfr13I	GGNCC	7 cut(s)	568, 848, 995, 996, 1566, 1732, 1824
Cfr9I	CCCGGG	2 cut(s)	355, 1892
ClaI	ATCGAT	1 cut(s)	495
CpoI	CGGWCCG	1 cut(s)	1566
CseI	GACGC	3 cut(s)	127, 1875, 1886
Csp6I	GTAC	4 cut(s)	298, 376, 727, 1297
CspAI	ACCGGT	1 cut(s)	545
CspI	CGGWCCG	1 cut(s)	1566
CviAII	CATG	5 cut(s)	124, 163, 203, 643, 1499
CviQI	GTAC	4 cut(s)	298, 376, 727, 1297
DdeI	CTNAG	5 cut(s)	188, 294, 963, 1199, 1449
DrdI	GACNNNNNNGTC	1 cut(s)	485
DseDI	GACNNNNNNGTC	1 cut(s)	485
EaeI	YGGCCR	5 cut(s)	199, 313, 531, 918, 1192
EagI	CGGCCG	1 cut(s)	531
Eam1104I	CTCTTC	1 cut(s)	1047
EarI	CTCTTC	1 cut(s)	1047
EciI	GGCGGA	1 cut(s)	205
Ecl136II	GAGCTC	4 cut(s)	82, 985, 1021, 1587
EclXI	CGGCCG	1 cut(s)	531
Eco147I	AGGCCT	1 cut(s)	602
Eco24I	GRGCYC	7 cut(s)	84, 156, 591, 987, 999, 1023, 1589
Eco31I	GGTCTC	1 cut(s)	1633
Eco32I	GATATC	1 cut(s)	499
Eco47I	GGWCC	3 cut(s)	568, 1566, 1732
Eco52I	CGGCCG	1 cut(s)	531
Eco53kI	GAGCTC	4 cut(s)	82, 985, 1021, 1587
Eco57I	CTGAAG	1 cut(s)	1802
Eco88I	CYCGRG	3 cut(s)	355, 1553, 1892
Eco91I	GGTNACC	1 cut(s)	1541
EcoICRI	GAGCTC	4 cut(s)	82, 985, 1021, 1587
EcoNI	CCTNNNNNAGG	1 cut(s)	1664
EcoO109I	RGGNCCY	2 cut(s)	568, 996
EcoO65I	GGTNACC	1 cut(s)	1541
EcoRI	GAATTC	4 cut(s)	437, 461, 1105, 1166
EcoRII	CCWGG	4 cut(s)	507, 597, 602, 1814
EcoRV	GATATC	1 cut(s)	499
EcoT38I	GRGCYC	7 cut(s)	84, 156, 591, 987, 999, 1023, 1589
Esp3I	CGTCTC	3 cut(s)	781, 1202, 1893
FaeI	CATG	5 cut(s)	127, 166, 206, 646, 1502
FaqI	GGGAC	5 cut(s)	275, 482, 1108, 1430, 1546
FatI	CATG	5 cut(s)	123, 162, 202, 642, 1498
FbaI	TGATCA	1 cut(s)	876
Fnu4HI	GCNGC	5 cut(s)	118, 313, 1616, 1866, 1871
FokI	GGATG	4 cut(s)	371, 1061, 1604, 1760
FriOI	GRGCYC	7 cut(s)	84, 156, 591, 987, 999, 1023, 1589
Fsp4HI	GCNGC	5 cut(s)	118, 313, 1616, 1866, 1871
FspBI	CTAG	4 cut(s)	1149, 1521, 1547, 1787
GlaI	GCGC	1 cut(s)	1869
GluI	GCNGC	5 cut(s)	118, 313, 1616, 1866, 1871
GsaI	CCCAGC	5 cut(s)	31, 59, 70, 372, 1333
GsuI	CTGGAG	2 cut(s)	1640, 1784
HaeII	RGCGCY	1 cut(s)	1871
HgaI	GACGC	3 cut(s)	127, 1875, 1886
HhaI	GCGC	1 cut(s)	1870
Hin1I	GRCGYC	2 cut(s)	138, 1886
Hin1II	CATG	5 cut(s)	127, 166, 206, 646, 1502
Hin6I	GCGC	1 cut(s)	1868
HinP1I	GCGC	1 cut(s)	1868
HincII	GTYRAC	1 cut(s)	1560
HindII	GTYRAC	1 cut(s)	1560
HindIII	AAGCTT	2 cut(s)	828, 1285
HphI	GGTGA	9 cut(s)	274, 535, 634, 642, 979, 1069, 1855, 1871, 1955
Hpy166II	GTNNAC	4 cut(s)	946, 1087, 1560, 1732
Hpy188III	TCNNGA	8 cut(s)	451, 580, 592, 955, 1345, 1553, 1721, 1763
Hpy8I	GTNNAC	4 cut(s)	946, 1087, 1560, 1732
HpyAV	CCTTC	8 cut(s)	217, 271, 283, 337, 1382, 1455, 1699, 1906
HpyCH4III	ACNGT	5 cut(s)	400, 448, 640, 1405, 1831
HpyCH4IV	ACGT	4 cut(s)	486, 729, 774, 1434
HpyCH4V	TGCA	5 cut(s)	1162, 1219, 1384, 1715, 1963
HpyF10VI	GCNNNNNNNGC	8 cut(s)	126, 135, 318, 982, 1159, 1607, 1621, 1669
HpyF3I	CTNAG	5 cut(s)	188, 294, 963, 1199, 1449
HpySE526I	ACGT	4 cut(s)	486, 729, 774, 1434
Hsp92I	GRCGYC	2 cut(s)	138, 1886
Hsp92II	CATG	5 cut(s)	127, 166, 206, 646, 1502
HspAI	GCGC	1 cut(s)	1868
KroI	GCCGGC	1 cut(s)	1239
KroNI	GCCGGC	1 cut(s)	1241
Ksp22I	TGATCA	1 cut(s)	876
Lsp1109I	GCAGC	3 cut(s)	129, 1602, 1857
LweI	GCATC	2 cut(s)	152, 393
MaeI	CTAG	4 cut(s)	1149, 1521, 1547, 1787
MaeII	ACGT	4 cut(s)	486, 729, 774, 1434
MaeIII	GTNAC	2 cut(s)	634, 1541
MboII	GAAGA	6 cut(s)	271, 418, 728, 1064, 1921, 1985
MflI	RGATCY	3 cut(s)	359, 671, 1073
MhlI	GDGCHC	9 cut(s)	84, 156, 591, 987, 999, 1023, 1589, 1612, 1802
MlyI	GAGTC	3 cut(s)	201, 1555, 1573
MmeI	TCCRAC	6 cut(s)	441, 469, 540, 764, 904, 1155
MreI	CGCCGGCG	1 cut(s)	1239
MroNI	GCCGGC	1 cut(s)	1239
MseI	TTAA	4 cut(s)	131, 683, 792, 1128
MslI	CAYNNNNRTG	1 cut(s)	641
Mva1269I	GAATGC	2 cut(s)	5, 1963
MvaI	CCWGG	4 cut(s)	509, 599, 604, 1816
MvnI	CGCG	1 cut(s)	1880
MwoI	GCNNNNNNNGC	8 cut(s)	126, 135, 318, 982, 1159, 1607, 1621, 1669
NaeI	GCCGGC	1 cut(s)	1241
NciI	CCSGG	8 cut(s)	356, 357, 530, 535, 751, 846, 1893, 1894
NgoMIV	GCCGGC	1 cut(s)	1239
NlaIII	CATG	5 cut(s)	127, 166, 206, 646, 1502
NlaIV	GGNNCC	5 cut(s)	361, 590, 997, 998, 1734
NmeAIII	GCCGAG	3 cut(s)	177, 288, 360
NmuCI	GTSAC	1 cut(s)	634
NspI	RCATGY	1 cut(s)	166
NspV	TTCGAA	2 cut(s)	459, 1139
PaeI	GCATGC	1 cut(s)	166
PaqCI	CACCTGC	1 cut(s)	1828
PasI	CCCWGGG	1 cut(s)	1815
PceI	AGGCCT	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	3 cut(s)	147, 483, 492
PctI	GAATGC	2 cut(s)	5, 1963
PdiI	GCCGGC	1 cut(s)	1241
PfeI	GAWTC	7 cut(s)	15, 228, 492, 617, 656, 1408, 1445
PflFI	GACNNNGTC	1 cut(s)	446
PinAI	ACCGGT	1 cut(s)	545
PkrI	GCNGC	5 cut(s)	119, 314, 1617, 1867, 1872
PleI	GAGTC	3 cut(s)	200, 1555, 1573
PpsI	GAGTC	3 cut(s)	200, 1555, 1573
PpuMI	RGGWCCY	1 cut(s)	568
PsiI	TTATAA	1 cut(s)	1902
Psp124BI	GAGCTC	4 cut(s)	84, 987, 1023, 1589
Psp5II	RGGWCCY	1 cut(s)	568
Psp6I	CCWGG	4 cut(s)	507, 597, 602, 1814
PspEI	GGTNACC	1 cut(s)	1541
PspFI	CCCAGC	5 cut(s)	27, 55, 66, 368, 1329
PspGI	CCWGG	4 cut(s)	507, 597, 602, 1814
PspN4I	GGNNCC	5 cut(s)	361, 590, 997, 998, 1734
PspOMI	GGGCCC	1 cut(s)	995
PspPI	GGNCC	7 cut(s)	568, 848, 995, 996, 1566, 1732, 1824
PspPPI	RGGWCCY	1 cut(s)	568
PsrI	GAACNNNNNNTAC	2 cut(s)	719, 751
PstI	CTGCAG	1 cut(s)	1164
PstNI	CAGNNNCTG	1 cut(s)	1159
PsuI	RGATCY	3 cut(s)	359, 671, 1073
PsyI	GACNNNGTC	1 cut(s)	446
RsaI	GTAC	4 cut(s)	299, 377, 728, 1298
RsaNI	GTAC	4 cut(s)	298, 376, 727, 1297
RseI	CAYNNNNRTG	1 cut(s)	641
Rsr2I	CGGWCCG	1 cut(s)	1566
RsrII	CGGWCCG	1 cut(s)	1566
SacI	GAGCTC	4 cut(s)	84, 987, 1023, 1589
SaqAI	TTAA	4 cut(s)	131, 683, 792, 1128
SatI	GCNGC	5 cut(s)	118, 313, 1616, 1866, 1871
Sau96I	GGNCC	7 cut(s)	568, 848, 995, 996, 1566, 1732, 1824
ScaI	AGTACT	1 cut(s)	1298
SchI	GAGTC	3 cut(s)	201, 1555, 1573
SduI	GDGCHC	9 cut(s)	84, 156, 591, 987, 999, 1023, 1589, 1612, 1802
SfaNI	GCATC	2 cut(s)	152, 393
SfcI	CTRYAG	1 cut(s)	1160
SfuI	TTCGAA	2 cut(s)	459, 1139
SgrAI	CRCCGGYG	2 cut(s)	545, 1239
SinI	GGWCC	3 cut(s)	568, 1566, 1732
SmaI	CCCGGG	2 cut(s)	357, 1894
SmiMI	CAYNNNNRTG	1 cut(s)	641
SmlI	CTYRAG	2 cut(s)	578, 1022
SmoI	CTYRAG	2 cut(s)	578, 1022
SphI	GCATGC	1 cut(s)	166
SseBI	AGGCCT	1 cut(s)	602
SsiI	CCGC	6 cut(s)	216, 312, 1124, 1865, 1880, 1929
SspMI	CTAG	4 cut(s)	1149, 1521, 1547, 1787
SstI	GAGCTC	4 cut(s)	84, 987, 1023, 1589
StuI	AGGCCT	1 cut(s)	602
TaaI	ACNGT	5 cut(s)	400, 448, 640, 1405, 1831
TaiI	ACGT	4 cut(s)	489, 732, 777, 1437
TaqII	GACCGA	1 cut(s)	1466
TatI	WGTACW	1 cut(s)	1296
TauI	GCSGC	2 cut(s)	315, 1868
TfiI	GAWTC	7 cut(s)	15, 228, 492, 617, 656, 1408, 1445
Tru1I	TTAA	4 cut(s)	131, 683, 792, 1128
Tru9I	TTAA	4 cut(s)	131, 683, 792, 1128
TscAI	CASTG	3 cut(s)	643, 1542, 1836
TseFI	GTSAC	1 cut(s)	634
TseI	GCWGC	3 cut(s)	117, 1615, 1870
Tsp45I	GTSAC	1 cut(s)	634
TspDTI	ATGAA	6 cut(s)	7, 319, 812, 1065, 1400, 1469
TspGWI	ACGGA	3 cut(s)	816, 886, 1473
TspMI	CCCGGG	2 cut(s)	355, 1892
TspRI	CASTG	3 cut(s)	643, 1542, 1836
Tth111I	GACNNNGTC	1 cut(s)	446
VpaK11BI	GGWCC	3 cut(s)	568, 1566, 1732
XagI	CCTNNNNNAGG	1 cut(s)	1664
XapI	RAATTY	8 cut(s)	437, 461, 553, 820, 1105, 1134, 1166, 1210
XceI	RCATGY	1 cut(s)	166
XmaI	CCCGGG	2 cut(s)	355, 1892
XspI	CTAG	4 cut(s)	1149, 1521, 1547, 1787
ZrmI	AGTACT	1 cut(s)	1298

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.