pycom01g15830

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001385
Physical Location & Seq
Reverse (-)
15576859 .. 15578298
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g15830.1

Sequence Viewer

Length: 1440 bp
ATGCTTTCACCCGATTCGATCAAACCCAAATCCCCGATTTTAACATTCGATGTCGATCTTTCTCCAATTTTGAAAGATTTTATGTACGTTGGGTTCTCTGCTTCGACGGGGTTGCTCGCCAGCTCCCACTACGTCTCCGGTTGGAGCTTTAAGATGAACGGAGAAGCCAAATCTCTGGATTTAGAAGCTCTGCCTAAACTTCCCGGCCCGAAAAAGAAGCATTCATCAGCGTTGATCGTTGGCGTTTCTCTTTCTGTTCTTGTTTTCTTTATCTTGGCCGCCGGATTGGGCTTCTACATTGTTCACAGGATCAAGAACGCCGAGGTGATCGAGCCGTGGGAGCTCGACATTGGGCCTCATAGATTCAGATACAATGAGCTGAAGCAGGCCACCAGAGGGTTCAGGGATAAAGAGGTGATCGGGTTTGGTGGATTTGGTAAGGTTTACAAAGGGACACTGCGGAATTCGGAAACGCAAGTGGCGGTTAAGCGAATTTCGCACGAGTCGAGGCAGGGTCTGCAGGAATTCGTGTCGGAAATTGCTACCATTGGCCGTCTCAGGCACAGGAATTTAGTGCAGTTGTTGGGGTGGTGTCGCCGGCGCGGAGATTTGTTGCTTGTGTATGATTTTATGCCTAATGGGAGCTTGGATAAATATTTATTTGAGAAGCCAAAAGCAATTTTGAGCTGGGAACAGAGGTTCAAGATTGCTCAAAATGTAGCTTCTGGCTTATTGTATTTGCACGAAGGTTGGGAGCAGACTGTGATTCATAGGGACATAAAAGCAGGAAACGTGCTTTTGGATTCTGAGATGAATGGAAGGCTTGGAGATTTCGGTCTTGCTAAATTGTATGAGCATGGAGCAAACCCTACAACAACTAGGGTTGTTGGGACACTGGGTTACCTCGCTCCGGAGTTGACTCGGACCGGAAAACCGACTCCGAGCTCGGATGTGTTTGCTCTTGGTGCTCTCCTGCTGGAGCTGGTGTGTGGGAGGAGACCCATTGAACAAAAAGCCCTGCCGGAGGAGGTGATATTGGTTGATTGGGTTTGGGAGAAGTGGAAAGCTGGTGCAATTCTTGAAGTTGTGGACCCCAGATTGGGGGATGAGTTTGATGATCTGGAGGCTGTGGTGGTGCTGAAGCTAGGGCTGATGTGCTCAAATAATACCCCCAAGGCAAGGCCCACAATGAGGCAGGTGGTGAGGTACTTGGAGGGTGAGGCAGCGTTGCCGGACACAGTGGCGTCTCCCGGGGCTTATGATGGGAAGAAGGGTGTTGAGTTCGAGGATTATGTGCATTCTTATCCCACATCATCAGGTTTTGTGAAGGCAAGTGATTGGTCCTGTGATGATGCGGATGTTGATTTCGACGGCTGTTCTGGCTCGCCTCCCTTAGTATCTGGTAGTCGTGGAGGTGGTAGTGACAGGTACCCAAGCTAG

Protein Analysis

480

Amino Acids

52.91

Weight (kDa)

6.58

Isoelectric Point (pI)

34.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 7 - 65 3.2e-14 Legume lectin domain
Pkinase PF00069 135 - 401 1.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 137 - 404 9.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1186
Acc36I ACCTGC 1 cut(s) 1186
Acc65I GGTACC 1 cut(s) 1428
AccB1I GGYRCC 1 cut(s) 1428
AccII CGCG 1 cut(s) 603
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 5 cut(s) 279, 460, 482, 603, 1355
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 2 cut(s) 276, 550
AcsI RAATTY 4 cut(s) 463, 492, 524, 568
AcuI CTGAAG 2 cut(s) 401, 1160
AcyI GRCGYC 1 cut(s) 1244
AfaI GTAC 3 cut(s) 86, 1208, 1430
AfiI CCNNNNNNNGG 8 cut(s) 396, 910, 1024, 1099, 1100, 1101, 1179, 1191
AgsI TTSAA 4 cut(s) 73, 703, 1007, 1082
AloI GAACNNNNNNTCC 2 cut(s) 683, 715
Alw21I GWGCWC 4 cut(s) 345, 947, 970, 1160
Alw26I GTCTC 4 cut(s) 139, 560, 991, 1251
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 517
Ama87I CYCGRG 1 cut(s) 1250
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 6 cut(s) 205, 276, 353, 387, 550, 1181
ApeKI GCWGC 1 cut(s) 1223
ApoI RAATTY 4 cut(s) 463, 492, 524, 568
Asp718I GGTACC 1 cut(s) 1428
AspLEI GCGC 1 cut(s) 603
AspS9I GGNCC 6 cut(s) 206, 353, 924, 1090, 1182, 1341
AsuC2I CCSGG 3 cut(s) 204, 1251, 1252
AsuHPI GGTGA 5 cut(s) 337, 427, 1042, 1213, 1229
AvaI CYCGRG 1 cut(s) 1250
AvaII GGWCC 3 cut(s) 924, 1090, 1341
BanI GGYRCC 1 cut(s) 1428
BanII GRGCYC 2 cut(s) 345, 947
BauI CACGAG 1 cut(s) 500
Bbv12I GWGCWC 4 cut(s) 345, 947, 970, 1160
BbvI GCAGC 1 cut(s) 1235
BccI CCATC 1 cut(s) 1256
BceAI ACGGC 3 cut(s) 319, 537, 1387
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BcnI CCSGG 3 cut(s) 204, 1251, 1252
BcoDI GTCTC 4 cut(s) 139, 560, 991, 1251
BfaI CTAG 3 cut(s) 879, 1145, 1438
BfmI CTRYAG 1 cut(s) 518
BfuAI ACCTGC 1 cut(s) 1186
BisI GCNGC 2 cut(s) 279, 1224
BlsI GCNGC 2 cut(s) 280, 1225
Bme1390I CCNGG 3 cut(s) 204, 1251, 1252
Bme18I GGWCC 3 cut(s) 924, 1090, 1341
BmeT110I CYCGRG 1 cut(s) 1250
BmgT120I GGNCC 6 cut(s) 206, 353, 924, 1090, 1182, 1341
BmiI GGNNCC 2 cut(s) 1092, 1430
BmrFI CCNGG 3 cut(s) 204, 1251, 1252
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 1 cut(s) 1342
BmuI ACTGGG 1 cut(s) 905
BpmI CTGGAG 2 cut(s) 998, 1142
BpuMI CCSGG 3 cut(s) 204, 1251, 1252
BsaBI GATNNNNATC 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 1244
BsaI GGTCTC 1 cut(s) 991
BsaJI CCNNGG 5 cut(s) 321, 335, 1173, 1250, 1251
BsaWI WCCGGW 3 cut(s) 137, 910, 926
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 971, 1001
Bsc4I CCNNNNNNNGG 8 cut(s) 396, 910, 1024, 1099, 1100, 1101, 1179, 1191
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 54
BseAI TCCGGA 1 cut(s) 910
BseDI CCNNGG 5 cut(s) 321, 335, 1173, 1250, 1251
BseGI GGATG 3 cut(s) 955, 1111, 1363
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 8 cut(s) 396, 910, 1024, 1099, 1100, 1101, 1179, 1191
BseMII CTCAG 2 cut(s) 571, 798
BseNI ACTGG 1 cut(s) 900
BseRI GAGGAG 2 cut(s) 1009, 1040
BseXI GCAGC 1 cut(s) 1235
BseYI CCCAGC 1 cut(s) 687
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 6 cut(s) 207, 278, 355, 389, 552, 1183
BshNI GGYRCC 1 cut(s) 1428
BsiHKAI GWGCWC 4 cut(s) 345, 947, 970, 1160
BsiHKCI CYCGRG 1 cut(s) 1250
BsiSI CCGG 9 cut(s) 138, 204, 282, 598, 911, 927, 1022, 1232, 1251
BslFI GGGAC 3 cut(s) 466, 788, 904
BslI CCNNNNNNNGG 8 cut(s) 396, 910, 1024, 1099, 1100, 1101, 1179, 1191
BsmAI GTCTC 4 cut(s) 139, 560, 991, 1251
BsmBI CGTCTC 3 cut(s) 139, 560, 1251
BsmFI GGGAC 3 cut(s) 466, 788, 904
BsmI GAATGC 2 cut(s) 220, 1297
BsnI GGCC 6 cut(s) 207, 278, 355, 389, 552, 1183
Bso31I GGTCTC 1 cut(s) 991
BsoBI CYCGRG 1 cut(s) 1250
Bsp1286I GDGCHC 4 cut(s) 345, 947, 970, 1160
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
BspACI CCGC 5 cut(s) 279, 460, 482, 603, 1355
BspANI GGCC 6 cut(s) 207, 278, 355, 389, 552, 1183
BspCNI CTCAG 2 cut(s) 570, 799
BspEI TCCGGA 1 cut(s) 910
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 2 cut(s) 1092, 1430
BspMAI CTGCAG 1 cut(s) 522
BspMI ACCTGC 1 cut(s) 1186
BspPI GGATC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 1428
BspTNI GGTCTC 1 cut(s) 991
BsrFI RCCGGY 1 cut(s) 597
BsrI ACTGG 1 cut(s) 900
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 5 cut(s) 321, 335, 1173, 1250, 1251
BssMI GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
BssNI GRCGYC 1 cut(s) 1244
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 1173
Bst2BI CACGAG 1 cut(s) 500
Bst4CI ACNGT 2 cut(s) 763, 1240
BstACI GRCGYC 1 cut(s) 1244
BstAPI GCANNNNNTGC 1 cut(s) 517
BstC8I GCNNGC 5 cut(s) 117, 121, 387, 599, 1385
BstDEI CTNAG 3 cut(s) 557, 807, 1393
BstDSI CCRYGG 1 cut(s) 335
BstEII GGTNACC 1 cut(s) 899
BstENI CCTNNNNNAGG 1 cut(s) 1022
BstF5I GGATG 3 cut(s) 955, 1111, 1363
BstFNI CGCG 1 cut(s) 603
BstHHI GCGC 1 cut(s) 603
BstKTI GATC 7 cut(s) 21, 58, 237, 312, 330, 420, 1120
BstMAI GTCTC 4 cut(s) 139, 560, 991, 1251
BstMBI GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
BstMWI GCNNNNNNNGC 5 cut(s) 340, 496, 517, 965, 1380
BstPI GGTNACC 1 cut(s) 899
BstSCI CCNGG 3 cut(s) 202, 1249, 1250
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 1 cut(s) 1235
BstXI CCANNNNNNTGG 1 cut(s) 175
BsuRI GGCC 6 cut(s) 207, 278, 355, 389, 552, 1183
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 3 cut(s) 955, 1111, 1363
BtsI GCAGTG 1 cut(s) 455
BtsIMutI CAGTG 3 cut(s) 455, 893, 1245
BveI ACCTGC 1 cut(s) 1186
Cac8I GCNNGC 5 cut(s) 117, 121, 387, 599, 1385
CaiI CAGNNNCTG 1 cut(s) 517
CfoI GCGC 1 cut(s) 603
Cfr10I RCCGGY 1 cut(s) 597
Cfr13I GGNCC 6 cut(s) 206, 353, 924, 1090, 1182, 1341
Cfr9I CCCGGG 1 cut(s) 1250
CpoI CGGWCCG 1 cut(s) 924
CseI GACGC 1 cut(s) 1233
Csp6I GTAC 3 cut(s) 85, 1207, 1429
CspI CGGWCCG 1 cut(s) 924
CviAII CATG 1 cut(s) 857
CviQI GTAC 3 cut(s) 85, 1207, 1429
DdeI CTNAG 3 cut(s) 557, 807, 1393
DpnI GATC 7 cut(s) 20, 57, 236, 311, 329, 419, 1119
DpnII GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
EaeI YGGCCR 2 cut(s) 276, 550
Ecl136II GAGCTC 2 cut(s) 343, 945
Eco130I CCWWGG 1 cut(s) 1173
Eco24I GRGCYC 2 cut(s) 345, 947
Eco31I GGTCTC 1 cut(s) 991
Eco47I GGWCC 3 cut(s) 924, 1090, 1341
Eco53kI GAGCTC 2 cut(s) 343, 945
Eco57I CTGAAG 2 cut(s) 401, 1160
Eco88I CYCGRG 1 cut(s) 1250
Eco91I GGTNACC 1 cut(s) 899
EcoICRI GAGCTC 2 cut(s) 343, 945
EcoNI CCTNNNNNAGG 1 cut(s) 1022
EcoO65I GGTNACC 1 cut(s) 899
EcoRI GAATTC 2 cut(s) 463, 524
EcoT14I CCWWGG 1 cut(s) 1173
EcoT38I GRGCYC 2 cut(s) 345, 947
ErhI CCWWGG 1 cut(s) 1173
Esp3I CGTCTC 3 cut(s) 139, 560, 1251
FaeI CATG 1 cut(s) 860
FaqI GGGAC 3 cut(s) 466, 788, 904
FatI CATG 1 cut(s) 856
Fnu4HI GCNGC 2 cut(s) 279, 1224
FokI GGATG 3 cut(s) 962, 1118, 1370
FriOI GRGCYC 2 cut(s) 345, 947
Fsp4HI GCNGC 2 cut(s) 279, 1224
FspBI CTAG 3 cut(s) 879, 1145, 1438
GlaI GCGC 1 cut(s) 602
GluI GCNGC 2 cut(s) 279, 1224
GsaI CCCAGC 1 cut(s) 691
GsuI CTGGAG 2 cut(s) 998, 1142
HaeIII GGCC 6 cut(s) 207, 278, 355, 389, 552, 1183
HapII CCGG 9 cut(s) 138, 204, 282, 598, 911, 927, 1022, 1232, 1251
HgaI GACGC 1 cut(s) 1233
HhaI GCGC 1 cut(s) 603
Hin1I GRCGYC 1 cut(s) 1244
Hin1II CATG 1 cut(s) 860
Hin6I GCGC 1 cut(s) 601
HinP1I GCGC 1 cut(s) 601
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HinfI GANTC 7 cut(s) 14, 363, 503, 766, 803, 919, 937
HpaII CCGG 9 cut(s) 138, 204, 282, 598, 911, 927, 1022, 1232, 1251
HphI GGTGA 5 cut(s) 337, 427, 1042, 1213, 1229
Hpy166II GTNNAC 4 cut(s) 304, 445, 918, 1090
Hpy188I TCNGA 7 cut(s) 368, 469, 535, 808, 924, 942, 949
Hpy188III TCNNGA 6 cut(s) 176, 313, 703, 911, 1079, 1121
Hpy8I GTNNAC 4 cut(s) 304, 445, 918, 1090
Hpy99I CGWCG 2 cut(s) 109, 1373
HpyAV CCTTC 4 cut(s) 740, 813, 1264, 1321
HpyCH4III ACNGT 2 cut(s) 763, 1240
HpyCH4IV ACGT 3 cut(s) 87, 132, 792
HpyCH4V TGCA 5 cut(s) 520, 577, 742, 1073, 1297
HpyF10VI GCNNNNNNNGC 5 cut(s) 340, 496, 517, 965, 1380
HpyF3I CTNAG 3 cut(s) 557, 807, 1393
HpySE526I ACGT 3 cut(s) 87, 132, 792
Hsp92I GRCGYC 1 cut(s) 1244
Hsp92II CATG 1 cut(s) 860
HspAI GCGC 1 cut(s) 601
Kpn2I TCCGGA 1 cut(s) 910
KpnI GGTACC 1 cut(s) 1432
KroI GCCGGC 1 cut(s) 597
KroNI GCCGGC 1 cut(s) 599
Kzo9I GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
LmnI GCTCC 8 cut(s) 128, 144, 340, 642, 754, 860, 913, 979
Lsp1109I GCAGC 1 cut(s) 1235
LweI GCATC 1 cut(s) 1342
MaeI CTAG 3 cut(s) 879, 1145, 1438
MaeII ACGT 3 cut(s) 87, 132, 792
MaeIII GTNAC 2 cut(s) 899, 1421
MalI GATC 7 cut(s) 20, 57, 236, 311, 329, 419, 1119
MboI GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
MboII GAAGA 1 cut(s) 1279
MhlI GDGCHC 4 cut(s) 345, 947, 970, 1160
MluCI AATT 9 cut(s) 66, 463, 492, 524, 537, 568, 678, 845, 1074
MlyI GAGTC 3 cut(s) 512, 913, 931
MmeI TCCRAC 2 cut(s) 122, 513
MreI CGCCGGCG 1 cut(s) 597
MroI TCCGGA 1 cut(s) 910
MroNI GCCGGC 1 cut(s) 597
MseI TTAA 3 cut(s) 41, 150, 486
MspI CCGG 9 cut(s) 138, 204, 282, 598, 911, 927, 1022, 1232, 1251
MspR9I CCNGG 3 cut(s) 204, 1251, 1252
Mva1269I GAATGC 2 cut(s) 220, 1297
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 5 cut(s) 340, 496, 517, 965, 1380
NaeI GCCGGC 1 cut(s) 599
NciI CCSGG 3 cut(s) 204, 1251, 1252
NdeII GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
NgoMIV GCCGGC 1 cut(s) 597
NlaIII CATG 1 cut(s) 860
NlaIV GGNNCC 2 cut(s) 1092, 1430
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 1421
PaqCI CACCTGC 1 cut(s) 1186
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PctI GAATGC 2 cut(s) 220, 1297
PdiI GCCGGC 1 cut(s) 599
PfeI GAWTC 4 cut(s) 14, 363, 766, 803
PkrI GCNGC 2 cut(s) 280, 1225
PleI GAGTC 3 cut(s) 511, 913, 931
PpsI GAGTC 3 cut(s) 511, 913, 931
Psp124BI GAGCTC 2 cut(s) 345, 947
PspEI GGTNACC 1 cut(s) 899
PspFI CCCAGC 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 1092, 1430
PspPI GGNCC 6 cut(s) 206, 353, 924, 1090, 1182, 1341
PsrI GAACNNNNNNTAC 2 cut(s) 77, 109
PstI CTGCAG 1 cut(s) 522
PstNI CAGNNNCTG 1 cut(s) 517
RsaI GTAC 3 cut(s) 86, 1208, 1430
RsaNI GTAC 3 cut(s) 85, 1207, 1429
Rsr2I CGGWCCG 1 cut(s) 924
RsrII CGGWCCG 1 cut(s) 924
SacI GAGCTC 2 cut(s) 345, 947
SaqAI TTAA 3 cut(s) 41, 150, 486
SatI GCNGC 2 cut(s) 279, 1224
Sau3AI GATC 7 cut(s) 18, 55, 234, 309, 327, 417, 1117
Sau96I GGNCC 6 cut(s) 206, 353, 924, 1090, 1182, 1341
SchI GAGTC 3 cut(s) 512, 913, 931
ScrFI CCNGG 3 cut(s) 204, 1251, 1252
SduI GDGCHC 4 cut(s) 345, 947, 970, 1160
SfaNI GCATC 1 cut(s) 1342
SfcI CTRYAG 1 cut(s) 518
SgrAI CRCCGGYG 1 cut(s) 597
SinI GGWCC 3 cut(s) 924, 1090, 1341
SmaI CCCGGG 1 cut(s) 1252
Sse9I AATT 9 cut(s) 66, 463, 492, 524, 537, 568, 678, 845, 1074
SsiI CCGC 5 cut(s) 279, 460, 482, 603, 1355
SspI AATATT 1 cut(s) 656
SspMI CTAG 3 cut(s) 879, 1145, 1438
SstI GAGCTC 2 cut(s) 345, 947
StyD4I CCNGG 3 cut(s) 202, 1249, 1250
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 2 cut(s) 763, 1240
TaiI ACGT 3 cut(s) 90, 135, 795
TaqI TCGA 9 cut(s) 17, 48, 54, 104, 330, 345, 506, 1284, 1368
TaqII GACCGA 1 cut(s) 824
TasI AATT 9 cut(s) 66, 463, 492, 524, 537, 568, 678, 845, 1074
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 4 cut(s) 14, 363, 766, 803
Tru1I TTAA 3 cut(s) 41, 150, 486
Tru9I TTAA 3 cut(s) 41, 150, 486
TscAI CASTG 3 cut(s) 462, 900, 1245
TseFI GTSAC 1 cut(s) 1421
TseI GCWGC 1 cut(s) 1223
Tsp45I GTSAC 1 cut(s) 1421
TspDTI ATGAA 4 cut(s) 170, 213, 758, 827
TspGWI ACGGA 1 cut(s) 174
TspMI CCCGGG 1 cut(s) 1250
TspRI CASTG 3 cut(s) 462, 900, 1245
VpaK11BI GGWCC 3 cut(s) 924, 1090, 1341
XagI CCTNNNNNAGG 1 cut(s) 1022
XapI RAATTY 4 cut(s) 463, 492, 524, 568
XmaI CCCGGG 1 cut(s) 1250
XspI CTAG 3 cut(s) 879, 1145, 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.