MD01G1189500.v1.1

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0010601
Physical Location & Seq
Forward (+)
28842375 .. 28844185
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1189500.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCCTCTTTTTCTACTCCTCCTCCTCCTCCTTCTTCTTCCCGTGGTCGTTGGATTTATGATGTGTTCATTAATTTCGGGAGCGAAGACACTCGCAAGATCTTTATCGGCCATCTTTACAAAGCGCTGGAGCAGAAATCAATCAAAACCTTCATTGATGGCGATGATCCGGATCTCTTGACAACTATCCAAGTGTCAAAGATCTCGATTGTAGTTTTTTCTAAAAACTATGCTTCTTCTTCCACGCGGTGCTTAAAACAACTTGTTCATATCCTAGATCATATGGATCCAGAGAAGCATATAGTGGTGCCCATTTTCTACCAAGTAGATCCGTCTCATGTTCGTAAACTCGCGGGAAGTCTTCAACCTGATCACGGTTCTAACGCCAGCATCGAAGAGATGCAGAACTGGAGGTCCGCTCTAACTAAAGCCGCCGGTATATCTGGCTGGGATTCACAAGAGTCTATGGATGATGCCAAGTTTATTGAGGACATTGTGCAAGATATTTTTAGTAAATTGCTCCAACTCAGCAATGTTTTCATCAATTTTAGAGGTGAAGACACTCGCCTCAGTTTTGTCGCCCATCTCCACAAAGCTCTGTATCAGAAAGCAATCCACGCCTTCAGCGATCTTGGAGAGCTTAATAAAGGCGATGAGATTTCGAATCTCTTCAACGATATTCAAAAATCAAGTATTTCGATCGTAATTTTTTCCAAAGGATATGCCCAATCCACATGGTGCTTGAGAGAACTGGTCGAAATCCTGGAATGTGTGGACAGAAAAATTCAGAAAGTGTGTCCCGTTTTCTATGGAGTATCTCCATCTGAAGTTCGTAAAATCACCGGTCCTTTTGGGGATGCTTTTTATCGATACAATGACAATTCTCGCTTCGACGTGGAAGAGGTGAAGAGATGGCGCTACGCTCTCACTAGAGCCGCCAATTATAGTGGATTTGATCGCATTCATTCCAAGAATGATGCCGAGCTTATTGAGAAAATTGTACAACACACTTGGAGAGCCTGCCGTGACTTCGACGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

347

Amino Acids

39.53

Weight (kDa)

6.42

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 176 5.2e-41 TIR domain
TIR_2 PF13676 22 - 111 4.9e-06 TIR domain
TIR PF01582 179 - 340 7.7e-42 TIR domain
TIR_2 PF13676 179 - 274 2.8e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 307
AccBSI CCGCTC 1 cut(s) 419
AccII CGCG 2 cut(s) 247, 353
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 5 cut(s) 247, 353, 417, 432, 936
AclWI GGATC 5 cut(s) 161, 180, 281, 294, 323
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 783
AcuI CTGAAG 2 cut(s) 607, 846
AdeI CACNNNGTG 2 cut(s) 249, 738
AfaI GTAC 1 cut(s) 1002
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 842
AgsI TTSAA 3 cut(s) 365, 673, 683
AjiI CACGTC 1 cut(s) 895
AjnI CCWGG 1 cut(s) 762
AloI GAACNNNNNNTCC 2 cut(s) 398, 430
AluBI AGCT 3 cut(s) 596, 640, 985
AluI AGCT 3 cut(s) 596, 640, 985
Alw26I GTCTC 1 cut(s) 339
AlwI GGATC 5 cut(s) 161, 180, 281, 294, 323
Aor13HI TCCGGA 1 cut(s) 169
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 2 cut(s) 3, 109
ApoI RAATTY 1 cut(s) 783
AseI ATTAAT 1 cut(s) 72
AsiGI ACCGGT 1 cut(s) 842
Asp700I GAANNNNTTC 1 cut(s) 668
AspLEI GCGC 2 cut(s) 127, 918
AspS9I GGNCC 2 cut(s) 414, 845
AsuHPI GGTGA 3 cut(s) 566, 832, 916
AsuII TTCGAA 1 cut(s) 662
AvaII GGWCC 2 cut(s) 414, 845
BaeGI GKGCMC 1 cut(s) 312
BamHI GGATCC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 307
BbsI GAAGAC 3 cut(s) 93, 353, 564
BccI CCATC 5 cut(s) 120, 152, 591, 829, 906
BceAI ACGGC 1 cut(s) 1008
BciT130I CCWGG 1 cut(s) 764
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 339
BfaI CTAG 2 cut(s) 275, 930
BfoI RGCGCY 2 cut(s) 128, 919
BglII AGATCT 2 cut(s) 99, 201
BisI GCNGC 2 cut(s) 432, 936
BlsI GCNGC 2 cut(s) 433, 937
Bme1390I CCNGG 1 cut(s) 764
Bme18I GGWCC 2 cut(s) 414, 845
BmgBI CACGTC 1 cut(s) 895
BmgT120I GGNCC 2 cut(s) 414, 845
BmiI GGNNCC 2 cut(s) 288, 309
BmrFI CCNGG 1 cut(s) 764
BmsI GCATC 5 cut(s) 390, 399, 463, 847, 967
BpiI GAAGAC 3 cut(s) 93, 353, 564
BplI GAGNNNNNCTC 2 cut(s) 403, 435
BpmI CTGGAG 2 cut(s) 149, 430
Bpu14I TTCGAA 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 763
Bsa29I ATCGAT 1 cut(s) 868
BsaBI GATNNNNATC 2 cut(s) 104, 171
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 2 cut(s) 169, 842
BsaXI ACNNNNNCTCC 2 cut(s) 7, 37
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse118I RCCGGY 2 cut(s) 434, 842
Bse1I ACTGG 2 cut(s) 413, 756
Bse3DI GCAATG 1 cut(s) 538
Bse8I GATNNNNATC 2 cut(s) 104, 171
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 764
BseCI ATCGAT 1 cut(s) 868
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 2 cut(s) 475, 862
BseJI GATNNNNATC 2 cut(s) 104, 171
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMI GCAATG 1 cut(s) 538
BseMII CTCAG 2 cut(s) 541, 583
BseNI ACTGG 2 cut(s) 413, 756
BseRI GAGGAG 4 cut(s) 9, 12, 15, 18
BseSI GKGCMC 1 cut(s) 312
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 247, 353
Bsh1285I CGRYCG 1 cut(s) 702
BshFI GGCC 2 cut(s) 5, 111
BshNI GGYRCC 1 cut(s) 307
BshTI ACCGGT 1 cut(s) 842
BshVI ATCGAT 1 cut(s) 868
BsiEI CGRYCG 1 cut(s) 702
BsiSI CCGG 3 cut(s) 170, 435, 843
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 339
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 783
BsmI GAATGC 1 cut(s) 960
BsnI GGCC 2 cut(s) 5, 111
Bsp119I TTCGAA 1 cut(s) 662
Bsp1286I GDGCHC 1 cut(s) 312
Bsp13I TCCGGA 1 cut(s) 169
Bsp1407I TGTACA 1 cut(s) 1000
BspACI CCGC 5 cut(s) 247, 353, 417, 432, 936
BspANI GGCC 2 cut(s) 5, 111
BspCNI CTCAG 2 cut(s) 540, 582
BspDI ATCGAT 1 cut(s) 868
BspEI TCCGGA 1 cut(s) 169
BspFNI CGCG 2 cut(s) 247, 353
BspLI GGNNCC 2 cut(s) 288, 309
BspPI GGATC 5 cut(s) 161, 180, 281, 294, 323
BspT104I TTCGAA 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 307
BsrBI CCGCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 538
BsrFI RCCGGY 2 cut(s) 434, 842
BsrGI TGTACA 1 cut(s) 1000
BsrI ACTGG 2 cut(s) 413, 756
BssAI RCCGGY 2 cut(s) 434, 842
BssECI CCNNGG 1 cut(s) 43
Bst2UI CCWGG 1 cut(s) 764
Bst4CI ACNGT 1 cut(s) 377
Bst6I CTCTTC 4 cut(s) 390, 674, 894, 902
BstAUI TGTACA 1 cut(s) 1000
BstBI TTCGAA 1 cut(s) 662
BstC8I GCNNGC 2 cut(s) 388, 1021
BstDEI CTNAG 2 cut(s) 527, 569
BstDSI CCRYGG 1 cut(s) 43
BstF5I GGATG 2 cut(s) 475, 862
BstFNI CGCG 2 cut(s) 247, 353
BstH2I RGCGCY 2 cut(s) 128, 919
BstHHI GCGC 2 cut(s) 127, 918
BstMAI GTCTC 1 cut(s) 339
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstNI CCWGG 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 762
BstSLI GKGCMC 1 cut(s) 312
BstUI CGCG 2 cut(s) 247, 353
BstV2I GAAGAC 3 cut(s) 93, 353, 564
BstX2I RGATCY 5 cut(s) 99, 172, 201, 286, 328
BstYI RGATCY 5 cut(s) 99, 172, 201, 286, 328
Bsu15I ATCGAT 1 cut(s) 868
BsuRI GGCC 2 cut(s) 5, 111
BsuTUI ATCGAT 1 cut(s) 868
BtgI CCRYGG 1 cut(s) 43
BtgZI GCGATG 2 cut(s) 177, 666
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 2 cut(s) 475, 862
Cac8I GCNNGC 2 cut(s) 388, 1021
CfoI GCGC 2 cut(s) 127, 918
Cfr10I RCCGGY 2 cut(s) 434, 842
Cfr13I GGNCC 2 cut(s) 414, 845
ClaI ATCGAT 1 cut(s) 868
Csp6I GTAC 1 cut(s) 1001
CspAI ACCGGT 1 cut(s) 842
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviAII CATG 2 cut(s) 338, 735
CviJI RGCY 9 cut(s) 5, 111, 431, 447, 596, 640, 935, 985, 1019
CviKI_1 RGCY 9 cut(s) 5, 111, 431, 447, 596, 640, 935, 985, 1019
CviQI GTAC 1 cut(s) 1001
DdeI CTNAG 2 cut(s) 527, 569
DraIII CACNNNGTG 2 cut(s) 249, 738
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 4 cut(s) 390, 674, 894, 902
EarI CTCTTC 4 cut(s) 390, 674, 894, 902
Eco47I GGWCC 2 cut(s) 414, 845
Eco47III AGCGCT 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 607, 846
EcoRII CCWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 2 cut(s) 341, 738
FaqI GGGAC 1 cut(s) 783
FatI CATG 2 cut(s) 337, 734
FauI CCCGC 1 cut(s) 346
FauNDI CATATG 1 cut(s) 282
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 2 cut(s) 432, 936
FokI GGATG 2 cut(s) 482, 869
Fsp4HI GCNGC 2 cut(s) 432, 936
FspBI CTAG 2 cut(s) 275, 930
GlaI GCGC 2 cut(s) 126, 917
GluI GCNGC 2 cut(s) 432, 936
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 2 cut(s) 149, 430
HaeII RGCGCY 2 cut(s) 128, 919
HaeIII GGCC 2 cut(s) 5, 111
HapII CCGG 3 cut(s) 170, 435, 843
HhaI GCGC 2 cut(s) 127, 918
Hin1II CATG 2 cut(s) 341, 738
Hin6I GCGC 2 cut(s) 125, 916
HinP1I GCGC 2 cut(s) 125, 916
HinfI GANTC 3 cut(s) 452, 461, 664
HpaII CCGG 3 cut(s) 170, 435, 843
HphI GGTGA 3 cut(s) 566, 832, 916
Hpy166II GTNNAC 2 cut(s) 347, 775
Hpy188I TCNGA 3 cut(s) 606, 789, 826
Hpy188III TCNNGA 5 cut(s) 79, 170, 178, 205, 290
Hpy8I GTNNAC 2 cut(s) 347, 775
Hpy99I CGWCG 2 cut(s) 896, 1037
HpyAV CCTTC 3 cut(s) 42, 160, 631
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 2 cut(s) 894, 1035
HpyCH4V TGCA 2 cut(s) 403, 499
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
HpyF3I CTNAG 2 cut(s) 527, 569
HpySE526I ACGT 2 cut(s) 894, 1035
Hsp92II CATG 2 cut(s) 341, 738
HspAI GCGC 2 cut(s) 125, 916
Kpn2I TCCGGA 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 370
LmnI GCTCC 3 cut(s) 81, 130, 525
LweI GCATC 5 cut(s) 390, 399, 463, 847, 967
MaeI CTAG 2 cut(s) 275, 930
MaeII ACGT 2 cut(s) 894, 1035
MaeIII GTNAC 1 cut(s) 1025
MbiI CCGCTC 1 cut(s) 419
MflI RGATCY 5 cut(s) 99, 172, 201, 286, 328
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 8 cut(s) 73, 515, 544, 705, 783, 880, 940, 996
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 2 cut(s) 32, 547
MroI TCCGGA 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 668
MseI TTAA 4 cut(s) 72, 254, 642, 1039
MspI CCGG 3 cut(s) 170, 435, 843
MspR9I CCNGG 1 cut(s) 764
Mva1269I GAATGC 1 cut(s) 960
MvaI CCWGG 1 cut(s) 764
MvnI CGCG 2 cut(s) 247, 353
MwoI GCNNNNNNNGC 1 cut(s) 617
NdeI CATATG 1 cut(s) 282
NlaIII CATG 2 cut(s) 341, 738
NlaIV GGNNCC 2 cut(s) 288, 309
NmeAIII GCCGAG 1 cut(s) 1006
NmuCI GTSAC 1 cut(s) 1025
NspV TTCGAA 1 cut(s) 662
PcsI WCGNNNNNNNCGW 2 cut(s) 390, 624
PctI GAATGC 1 cut(s) 960
PdmI GAANNNNTTC 1 cut(s) 668
PfeI GAWTC 2 cut(s) 452, 664
PfoI TCCNGGA 1 cut(s) 762
PinAI ACCGGT 1 cut(s) 842
PkrI GCNGC 2 cut(s) 433, 937
Ple19I CGATCG 1 cut(s) 702
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
PshBI ATTAAT 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 762
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 1 cut(s) 762
PspN4I GGNNCC 2 cut(s) 288, 309
PspPI GGNCC 2 cut(s) 414, 845
PsuI RGATCY 5 cut(s) 99, 172, 201, 286, 328
PvuI CGATCG 1 cut(s) 702
RsaI GTAC 1 cut(s) 1002
RsaNI GTAC 1 cut(s) 1001
SaqAI TTAA 4 cut(s) 72, 254, 642, 1039
SatI GCNGC 2 cut(s) 432, 936
Sau96I GGNCC 2 cut(s) 414, 845
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 1 cut(s) 764
SduI GDGCHC 1 cut(s) 312
SfaNI GCATC 5 cut(s) 390, 399, 463, 847, 967
SfuI TTCGAA 1 cut(s) 662
SinI GGWCC 2 cut(s) 414, 845
SmlI CTYRAG 1 cut(s) 742
SmoI CTYRAG 1 cut(s) 742
Sse9I AATT 8 cut(s) 73, 515, 544, 705, 783, 880, 940, 996
SsiI CCGC 5 cut(s) 247, 353, 417, 432, 936
SspMI CTAG 2 cut(s) 275, 930
StyD4I CCNGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 2 cut(s) 897, 1038
TaqI TCGA 8 cut(s) 206, 393, 662, 698, 756, 868, 891, 1032
TasI AATT 8 cut(s) 73, 515, 544, 705, 783, 880, 940, 996
TatI WGTACW 1 cut(s) 1000
TauI GCSGC 2 cut(s) 434, 938
TfiI GAWTC 2 cut(s) 452, 664
Tru1I TTAA 4 cut(s) 72, 254, 642, 1039
Tru9I TTAA 4 cut(s) 72, 254, 642, 1039
TseFI GTSAC 1 cut(s) 1025
Tsp45I GTSAC 1 cut(s) 1025
TspDTI ATGAA 5 cut(s) 58, 142, 257, 529, 953
TspGWI ACGGA 1 cut(s) 321
VpaK11BI GGWCC 2 cut(s) 414, 845
VspI ATTAAT 1 cut(s) 72
XapI RAATTY 1 cut(s) 783
XmnI GAANNNNTTC 1 cut(s) 668
XspI CTAG 2 cut(s) 275, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.