Rorug05G0497800

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010601
Physical Location & Seq
Forward (+)
67945771 .. 67947634
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0497800.1

Sequence Viewer

Length: 435 bp
ATGGAGGGTCTGTGGCGGGCCGCGACGGGCCAGGACCCGAGCCCAGAAGACTACACCGGCATCGAATTCTGGTCCAACCCTGAACGCGCCGCCTGGCTCACCAAGCAAGGCGAGTACATCAAGACCTGGCGCCGCCGCTGGTTCGTCCTCAAGCAAGGCAAGCTCTTCTGGTTCAAGGACTCCCACGTCACCCCCTCCTCCACCCCACGCGGCGTCATCCCCGTCGGCACCTGCCTCACCGTCAAGGGCGCCGAGGACGTCCTCCACAAGCCCTGCGCCTTCGAGCTCTCCACCACCACCCACGACACCATGTACTTCATCGCCGATACTGAGAAGGAGAAGGAGGAGTGGATCAACTCCATCGGACGGTCCTTAGTCCAGCACTCCAGATCGCTCGCCGATTCCGAGGTCGTCGATTACGATAACCGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

144

Amino Acids

16.65

Weight (kDa)

6.09

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 30 - 123 1.3e-19 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 185
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 129, 227, 248
AccII CGCG 3 cut(s) 23, 87, 210
AciI CCGC 7 cut(s) 16, 21, 90, 133, 136, 210, 427
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 1 cut(s) 65
AcyI GRCGYC 4 cut(s) 130, 213, 249, 258
AfaI GTAC 2 cut(s) 116, 314
AfiI CCNNNNNNNGG 1 cut(s) 366
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 3 cut(s) 30, 92, 125
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw21I GWGCWC 1 cut(s) 288
AlwI GGATC 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 2 cut(s) 18, 28
ApoI RAATTY 1 cut(s) 65
AspLEI GCGC 4 cut(s) 89, 132, 251, 278
AspS9I GGNCC 5 cut(s) 18, 28, 34, 72, 369
AsuHPI GGTGA 3 cut(s) 91, 181, 229
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 3 cut(s) 34, 72, 369
BanI GGYRCC 3 cut(s) 129, 227, 248
BanII GRGCYC 2 cut(s) 44, 288
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 288
BccI CCATC 1 cut(s) 368
BciT130I CCWGG 3 cut(s) 32, 94, 127
BfoI RGCGCY 2 cut(s) 133, 252
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 5 cut(s) 21, 90, 133, 136, 211
BlsI GCNGC 5 cut(s) 22, 91, 134, 137, 212
Bme1390I CCNGG 3 cut(s) 32, 94, 127
Bme18I GGWCC 3 cut(s) 34, 72, 369
BmeT110I CYCGRG 1 cut(s) 37
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 5 cut(s) 18, 28, 34, 72, 369
BmiI GGNNCC 4 cut(s) 36, 131, 229, 250
BmrFI CCNGG 3 cut(s) 32, 94, 127
BmsI GCATC 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 370
BpuEI CTTGAG 1 cut(s) 134
BsaHI GRCGYC 4 cut(s) 130, 213, 249, 258
BsaJI CCNNGG 2 cut(s) 252, 405
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 366
Bse118I RCCGGY 2 cut(s) 56, 429
BseBI CCWGG 3 cut(s) 32, 94, 127
BseDI CCNNGG 2 cut(s) 252, 405
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 321
BseRI GAGGAG 2 cut(s) 187, 359
Bsh1236I CGCG 3 cut(s) 23, 87, 210
BshFI GGCC 2 cut(s) 20, 30
BshNI GGYRCC 3 cut(s) 129, 227, 248
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 57, 430
BslI CCNNNNNNNGG 1 cut(s) 366
BsnI GGCC 2 cut(s) 20, 30
BsoBI CYCGRG 1 cut(s) 37
Bsp1286I GDGCHC 2 cut(s) 44, 288
Bsp143I GATC 2 cut(s) 351, 389
BspACI CCGC 7 cut(s) 16, 21, 90, 133, 136, 210, 427
BspANI GGCC 2 cut(s) 20, 30
BspCNI CTCAG 1 cut(s) 322
BspFNI CGCG 3 cut(s) 23, 87, 210
BspLI GGNNCC 4 cut(s) 36, 131, 229, 250
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 359
BspQI GCTCTTC 1 cut(s) 170
BspT107I GGYRCC 3 cut(s) 129, 227, 248
BsrFI RCCGGY 2 cut(s) 56, 429
BssAI RCCGGY 2 cut(s) 56, 429
BssECI CCNNGG 2 cut(s) 252, 405
BssMI GATC 2 cut(s) 351, 389
BssNI GRCGYC 4 cut(s) 130, 213, 249, 258
Bst2UI CCWGG 3 cut(s) 32, 94, 127
Bst4CI ACNGT 2 cut(s) 241, 369
Bst6I CTCTTC 1 cut(s) 170
BstACI GRCGYC 4 cut(s) 130, 213, 249, 258
BstC8I GCNNGC 3 cut(s) 18, 161, 396
BstDEI CTNAG 2 cut(s) 330, 373
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 3 cut(s) 23, 87, 210
BstH2I RGCGCY 2 cut(s) 133, 252
BstHHI GCGC 4 cut(s) 89, 132, 251, 278
BstKTI GATC 2 cut(s) 354, 392
BstMBI GATC 2 cut(s) 351, 389
BstMWI GCNNNNNNNGC 2 cut(s) 103, 160
BstNI CCWGG 3 cut(s) 32, 94, 127
BstSCI CCNGG 3 cut(s) 30, 92, 125
BstUI CGCG 3 cut(s) 23, 87, 210
BstV2I GAAGAC 1 cut(s) 54
BsuRI GGCC 2 cut(s) 20, 30
BtgZI GCGATG 1 cut(s) 304
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 3 cut(s) 18, 161, 396
CfoI GCGC 4 cut(s) 89, 132, 251, 278
Cfr10I RCCGGY 2 cut(s) 56, 429
Cfr13I GGNCC 5 cut(s) 18, 28, 34, 72, 369
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 115, 313
CviAII CATG 1 cut(s) 310
CviJI RGCY 7 cut(s) 20, 30, 42, 97, 163, 271, 286
CviKI_1 RGCY 7 cut(s) 20, 30, 42, 97, 163, 271, 286
CviQI GTAC 2 cut(s) 115, 313
DdeI CTNAG 2 cut(s) 330, 373
DinI GGCGCC 2 cut(s) 131, 250
DpnI GATC 2 cut(s) 353, 391
DpnII GATC 2 cut(s) 351, 389
DrdI GACNNNNNNGTC 1 cut(s) 185
DseDI GACNNNNNNGTC 1 cut(s) 185
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 2 cut(s) 44, 288
Eco47I GGWCC 3 cut(s) 34, 72, 369
Eco53kI GAGCTC 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 1 cut(s) 65
EcoRII CCWGG 3 cut(s) 30, 92, 125
EcoT38I GRGCYC 2 cut(s) 44, 288
EgeI GGCGCC 2 cut(s) 131, 250
EheI GGCGCC 2 cut(s) 131, 250
FaeI CATG 1 cut(s) 313
FaiI YATR 1 cut(s) 311
FatI CATG 1 cut(s) 309
FauI CCCGC 1 cut(s) 9
Fnu4HI GCNGC 5 cut(s) 21, 90, 133, 136, 211
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 44, 288
Fsp4HI GCNGC 5 cut(s) 21, 90, 133, 136, 211
GlaI GCGC 4 cut(s) 88, 131, 250, 277
GluI GCNGC 5 cut(s) 21, 90, 133, 136, 211
GsuI CTGGAG 1 cut(s) 370
HaeII RGCGCY 2 cut(s) 133, 252
HaeIII GGCC 2 cut(s) 20, 30
HapII CCGG 2 cut(s) 57, 430
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 89, 132, 251, 278
Hin1I GRCGYC 4 cut(s) 130, 213, 249, 258
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 4 cut(s) 87, 130, 249, 276
HinP1I GCGC 4 cut(s) 87, 130, 249, 276
HinfI GANTC 2 cut(s) 179, 401
HpaII CCGG 2 cut(s) 57, 430
HphI GGTGA 3 cut(s) 91, 181, 229
Hpy188I TCNGA 2 cut(s) 365, 406
Hpy188III TCNNGA 2 cut(s) 121, 387
Hpy99I CGWCG 3 cut(s) 28, 227, 416
HpyAV CCTTC 3 cut(s) 289, 328, 334
HpyCH4III ACNGT 2 cut(s) 241, 369
HpyCH4IV ACGT 2 cut(s) 186, 258
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 160
HpyF3I CTNAG 2 cut(s) 330, 373
HpySE526I ACGT 2 cut(s) 186, 258
Hsp92I GRCGYC 4 cut(s) 130, 213, 249, 258
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 4 cut(s) 87, 130, 249, 276
KasI GGCGCC 2 cut(s) 129, 248
Kzo9I GATC 2 cut(s) 351, 389
LguI GCTCTTC 1 cut(s) 170
LweI GCATC 1 cut(s) 69
MaeII ACGT 2 cut(s) 186, 258
MaeIII GTNAC 1 cut(s) 187
MalI GATC 2 cut(s) 353, 391
MboI GATC 2 cut(s) 351, 389
MboII GAAGA 2 cut(s) 59, 157
MhlI GDGCHC 2 cut(s) 44, 288
MluCI AATT 1 cut(s) 65
Mly113I GGCGCC 2 cut(s) 130, 249
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 8 cut(s) 158, 205, 208, 245, 247, 272, 337, 400
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 2 cut(s) 57, 430
MspR9I CCNGG 3 cut(s) 32, 94, 127
MvaI CCWGG 3 cut(s) 32, 94, 127
MvnI CGCG 3 cut(s) 23, 87, 210
MwoI GCNNNNNNNGC 2 cut(s) 103, 160
NarI GGCGCC 2 cut(s) 130, 249
NdeII GATC 2 cut(s) 351, 389
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 4 cut(s) 36, 131, 229, 250
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 170
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 417
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 5 cut(s) 22, 91, 134, 137, 212
PleI GAGTC 1 cut(s) 173
PluTI GGCGCC 2 cut(s) 133, 252
PpsI GAGTC 1 cut(s) 173
PpuMI RGGWCCY 1 cut(s) 34
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 3 cut(s) 30, 92, 125
PspGI CCWGG 3 cut(s) 30, 92, 125
PspN4I GGNNCC 4 cut(s) 36, 131, 229, 250
PspPI GGNCC 5 cut(s) 18, 28, 34, 72, 369
PspPPI RGGWCCY 1 cut(s) 34
RsaI GTAC 2 cut(s) 116, 314
RsaNI GTAC 2 cut(s) 115, 313
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 170
SatI GCNGC 5 cut(s) 21, 90, 133, 136, 211
Sau3AI GATC 2 cut(s) 351, 389
Sau96I GGNCC 5 cut(s) 18, 28, 34, 72, 369
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 32, 94, 127
SduI GDGCHC 2 cut(s) 44, 288
SetI ASST 7 cut(s) 128, 165, 189, 233, 261, 288, 411
SfaNI GCATC 1 cut(s) 69
SfoI GGCGCC 2 cut(s) 131, 250
SgrAI CRCCGGYG 1 cut(s) 429
SinI GGWCC 3 cut(s) 34, 72, 369
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
Sse9I AATT 1 cut(s) 65
SsiI CCGC 7 cut(s) 16, 21, 90, 133, 136, 210, 427
SspDI GGCGCC 2 cut(s) 129, 248
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 3 cut(s) 30, 92, 125
TaaI ACNGT 2 cut(s) 241, 369
TaiI ACGT 2 cut(s) 189, 261
TaqI TCGA 3 cut(s) 63, 282, 414
TasI AATT 1 cut(s) 65
TatI WGTACW 2 cut(s) 114, 312
TauI GCSGC 5 cut(s) 23, 92, 135, 138, 213
TfiI GAWTC 1 cut(s) 401
TseFI GTSAC 1 cut(s) 187
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 1 cut(s) 307
VpaK11BI GGWCC 3 cut(s) 34, 72, 369
XapI RAATTY 1 cut(s) 65
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.