pycom01g20180

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010601
Physical Location & Seq
Forward (+)
18583973 .. 18585297
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20180.1

Sequence Viewer

Length: 1116 bp
ATGTTTTATTTTTTAAGTATATTGTTTGGCCTGACCAGTCATCTTCCTATCGATCGGACCAATATCCAGCAGTCCTTTTATAGGGTACTAATCAATCCAGTATTTCAGTTGTTATCCAGCGGTCCTTTTATAGGGGTACTAATATTGTGCTTGTTAATGGCTGCTTGTTCTGTATCATCCGGTGGTCGATATGATGTGTTCATCAATTTTAGACGCGAAGACACTCGCAAGATCTTTATCGGCCATCTTTACAAAGCGCTGGTTCAGAAATCAATATACACCTTCATTGATGGTGACGATCCGGAGCTCCCGACAACTATCCAAGAGTCAAGGCTCTCGATTGTAGTTTTTTCTAAAAACTATGCTTCTTCCACGCGGTGCTTAAAACAACTTGTACATATCCTAGATCGTGTGGATACAAAGAAGCATATAGTGGTGCCCATTTTCTATCAAATAGATCCGTCTGATCTTCGTAAACTCGCTGGAAGTTTTGCGAAAGCTTTTGCTCAACATGATCACGACCCTAACGCCAACATCGATGAGGTGCAGAGCTGGAGGTCCGCTCTAACTAAAGCCGCCAATTTTTCCGGTTGGGCTTCACGAGATTATAGGGATGATGCCAAGTTTATTGAGGGAGTTGTGCAAGATATTTTTAATCAGTTACTCCAATTCAGCAAAGTTTTCGTCTCTTTTAGAGGTGAAGACACTCGCAAGGGTTTTGCCGCCCATCTCTCCAAAGCTCTGCATCAGAATGTACTCAGCACCTTTAGAGATGTTTCAGCGGATATTAAAGGCGATGAATTCAGCAGTCTTTTCAAAGTTATTCAGCAGTCAAAAATTTCGATTGTAATTTTTTCCGAAAAATATGCTTATTCCCGTTGGTGCTTAGACGAGTTGGTCGCAATCCTGGAATGTGCAGACACAAACAATCAGATTGTGTTTCCCGTTTTCTATGGAGTAGATCCATCTGATGTTCGTAAAGTCAAAGGGCACTTTGGGGCTTTTGATAGACACATGGAACGTTACAGATACAATAAGGAAAAGGTGACGCTTTGGCGCTACGCTCTAGCTAGAGCCGCCAATTATCCTGGATTTGTACGCAATAATTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR

Protein Analysis

372

Amino Acids

42.59

Weight (kDa)

9.18

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 64 - 223 4.4e-44 TIR domain
TIR_2 PF13676 66 - 159 7.4e-07 TIR domain
TIR PF01582 227 - 369 9.8e-38 TIR domain
TIR_2 PF13676 227 - 322 3.1e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 896
AccB1I GGYRCC 1 cut(s) 436
AccBSI CCGCTC 1 cut(s) 563
AccII CGCG 2 cut(s) 216, 376
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 7 cut(s) 120, 376, 561, 576, 723, 782, 1077
AclI AACGTT 1 cut(s) 1021
AclWI GGATC 3 cut(s) 293, 452, 956
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 2 cut(s) 800, 837
AdeI CACNNNGTG 1 cut(s) 378
AfaI GTAC 5 cut(s) 87, 138, 396, 756, 1098
AfeI AGCGCT 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 81, 131
AgsI TTSAA 2 cut(s) 817, 1111
AjnI CCWGG 2 cut(s) 906, 1087
AluBI AGCT 5 cut(s) 307, 500, 552, 740, 1070
AluI AGCT 5 cut(s) 307, 500, 552, 740, 1070
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 3 cut(s) 293, 452, 956
Aor13HI TCCGGA 1 cut(s) 301
Aor51HI AGCGCT 1 cut(s) 258
AoxI GGCC 2 cut(s) 28, 241
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 2 cut(s) 800, 837
ArsI GACNNNNNNTTYG 2 cut(s) 669, 701
AspLEI GCGC 2 cut(s) 259, 1059
AspS9I GGNCC 3 cut(s) 57, 122, 558
AsuHPI GGTGA 3 cut(s) 305, 710, 1057
AvaII GGWCC 3 cut(s) 57, 122, 558
BaeGI GKGCMC 2 cut(s) 441, 993
BanI GGYRCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 309
BauI CACGAG 1 cut(s) 600
BbsI GAAGAC 2 cut(s) 225, 708
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 148
BccI CCATC 4 cut(s) 252, 284, 735, 973
BcgI CGANNNNNNTGC 4 cut(s) 664, 698, 848, 882
BciT130I CCWGG 2 cut(s) 908, 1089
BciVI GTATCC 1 cut(s) 409
BclI TGATCA 1 cut(s) 514
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 3 cut(s) 404, 1067, 1071
BfoI RGCGCY 2 cut(s) 260, 1060
BfuI GTATCC 1 cut(s) 409
BglII AGATCT 1 cut(s) 231
BisI GCNGC 4 cut(s) 162, 576, 723, 1077
BlsI GCNGC 4 cut(s) 163, 577, 724, 1078
Bme1390I CCNGG 2 cut(s) 908, 1089
Bme18I GGWCC 3 cut(s) 57, 122, 558
BmgT120I GGNCC 3 cut(s) 57, 122, 558
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 908, 1089
BmsI GCATC 2 cut(s) 607, 754
BpiI GAAGAC 2 cut(s) 225, 708
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 1 cut(s) 574
Bsa29I ATCGAT 2 cut(s) 51, 537
BsaBI GATNNNNATC 1 cut(s) 236
BsaWI WCCGGW 3 cut(s) 179, 301, 587
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 131
Bse1I ACTGG 2 cut(s) 36, 98
Bse8I GATNNNNATC 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 2 cut(s) 908, 1089
BseCI ATCGAT 2 cut(s) 51, 537
BseGI GGATG 2 cut(s) 176, 619
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 2 cut(s) 81, 131
BseMII CTCAG 1 cut(s) 772
BseNI ACTGG 2 cut(s) 36, 98
BseSI GKGCMC 2 cut(s) 441, 993
BseXI GCAGC 1 cut(s) 148
BsgI GTGCAG 2 cut(s) 566, 936
Bsh1236I CGCG 2 cut(s) 216, 376
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 2 cut(s) 30, 243
BshNI GGYRCC 1 cut(s) 436
BshVI ATCGAT 2 cut(s) 51, 537
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 3 cut(s) 180, 302, 588
BslI CCNNNNNNNGG 2 cut(s) 81, 131
BsmAI GTCTC 1 cut(s) 691
BsmBI CGTCTC 1 cut(s) 691
BsnI GGCC 2 cut(s) 30, 243
Bsp1286I GDGCHC 3 cut(s) 309, 441, 993
Bsp13I TCCGGA 1 cut(s) 301
Bsp1407I TGTACA 1 cut(s) 394
Bsp143I GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
BspACI CCGC 7 cut(s) 120, 376, 561, 576, 723, 782, 1077
BspANI GGCC 2 cut(s) 30, 243
BspCNI CTCAG 1 cut(s) 771
BspDI ATCGAT 2 cut(s) 51, 537
BspEI TCCGGA 1 cut(s) 301
BspFNI CGCG 2 cut(s) 216, 376
BspLI GGNNCC 1 cut(s) 438
BspPI GGATC 3 cut(s) 293, 452, 956
BspT107I GGYRCC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 563
BsrGI TGTACA 1 cut(s) 394
BsrI ACTGG 2 cut(s) 36, 98
BssMI GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
BssSI CACGAG 1 cut(s) 600
Bst2BI CACGAG 1 cut(s) 600
Bst2UI CCWGG 2 cut(s) 908, 1089
BstAUI TGTACA 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 758, 886
BstENI CCTNNNNNAGG 2 cut(s) 79, 129
BstF5I GGATG 2 cut(s) 176, 619
BstFNI CGCG 2 cut(s) 216, 376
BstH2I RGCGCY 2 cut(s) 260, 1060
BstHHI GCGC 2 cut(s) 259, 1059
BstKTI GATC 8 cut(s) 55, 234, 301, 409, 460, 469, 517, 964
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 1076
BstNI CCWGG 2 cut(s) 908, 1089
BstSCI CCNGG 2 cut(s) 906, 1087
BstSLI GKGCMC 2 cut(s) 441, 993
BstUI CGCG 2 cut(s) 216, 376
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 2 cut(s) 225, 708
BstX2I RGATCY 3 cut(s) 231, 457, 961
BstYI RGATCY 3 cut(s) 231, 457, 961
Bsu15I ATCGAT 2 cut(s) 51, 537
BsuI GTATCC 1 cut(s) 409
BsuRI GGCC 2 cut(s) 30, 243
BsuTUI ATCGAT 2 cut(s) 51, 537
BtgZI GCGATG 1 cut(s) 810
BtsCI GGATG 2 cut(s) 176, 619
CfoI GCGC 2 cut(s) 259, 1059
Cfr13I GGNCC 3 cut(s) 57, 122, 558
ClaI ATCGAT 2 cut(s) 51, 537
CseI GACGC 2 cut(s) 222, 1057
Csp6I GTAC 5 cut(s) 86, 137, 395, 755, 1097
CviAII CATG 2 cut(s) 512, 1015
CviQI GTAC 5 cut(s) 86, 137, 395, 755, 1097
DdeI CTNAG 2 cut(s) 758, 886
DpnI GATC 8 cut(s) 54, 233, 300, 408, 459, 468, 516, 963
DpnII GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
DraIII CACNNNGTG 1 cut(s) 378
DrdI GACNNNNNNGTC 1 cut(s) 896
DseDI GACNNNNNNGTC 1 cut(s) 896
EaeI YGGCCR 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 307
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 3 cut(s) 57, 122, 558
Eco47III AGCGCT 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 307
EcoNI CCTNNNNNAGG 2 cut(s) 79, 129
EcoRI GAATTC 1 cut(s) 800
EcoRII CCWGG 2 cut(s) 906, 1087
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 691
FaeI CATG 2 cut(s) 515, 1018
FatI CATG 2 cut(s) 511, 1014
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 4 cut(s) 162, 576, 723, 1077
FokI GGATG 2 cut(s) 163, 626
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 4 cut(s) 162, 576, 723, 1077
FspBI CTAG 3 cut(s) 404, 1067, 1071
GlaI GCGC 2 cut(s) 258, 1058
GluI GCNGC 4 cut(s) 162, 576, 723, 1077
GsuI CTGGAG 1 cut(s) 574
HaeII RGCGCY 2 cut(s) 260, 1060
HaeIII GGCC 2 cut(s) 30, 243
HapII CCGG 3 cut(s) 180, 302, 588
HgaI GACGC 2 cut(s) 222, 1057
HhaI GCGC 2 cut(s) 259, 1059
Hin1II CATG 2 cut(s) 515, 1018
Hin6I GCGC 2 cut(s) 257, 1057
HinP1I GCGC 2 cut(s) 257, 1057
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 1 cut(s) 326
HpaII CCGG 3 cut(s) 180, 302, 588
HphI GGTGA 3 cut(s) 305, 710, 1057
Hpy166II GTNNAC 1 cut(s) 476
Hpy188I TCNGA 7 cut(s) 57, 267, 466, 750, 859, 933, 970
Hpy188III TCNNGA 5 cut(s) 302, 310, 337, 518, 600
Hpy8I GTNNAC 1 cut(s) 476
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 1021
HpyCH4V TGCA 4 cut(s) 547, 643, 745, 917
HpyF10VI GCNNNNNNNGC 1 cut(s) 1076
HpyF3I CTNAG 2 cut(s) 758, 886
HpySE526I ACGT 1 cut(s) 1021
Hsp92II CATG 2 cut(s) 515, 1018
HspAI GCGC 2 cut(s) 257, 1057
Kpn2I TCCGGA 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
LmnI GCTCC 2 cut(s) 304, 312
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 607, 754
MaeI CTAG 3 cut(s) 404, 1067, 1071
MaeII ACGT 1 cut(s) 1021
MaeIII GTNAC 4 cut(s) 293, 660, 1022, 1045
MalI GATC 8 cut(s) 54, 233, 300, 408, 459, 468, 516, 963
MbiI CCGCTC 1 cut(s) 563
MboI GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
MboII GAAGA 5 cut(s) 35, 230, 360, 461, 713
MflI RGATCY 3 cut(s) 231, 457, 961
MhlI GDGCHC 3 cut(s) 309, 441, 993
MluCI AATT 8 cut(s) 205, 580, 668, 800, 837, 849, 1081, 1105
MlyI GAGTC 1 cut(s) 335
MnlI CCTC 4 cut(s) 535, 549, 625, 689
MroI TCCGGA 1 cut(s) 301
MseI TTAA 5 cut(s) 14, 155, 383, 654, 789
MslI CAYNNNNRTG 1 cut(s) 750
MspA1I CMGCKG 2 cut(s) 120, 782
MspI CCGG 3 cut(s) 180, 302, 588
MspR9I CCNGG 2 cut(s) 908, 1089
MvaI CCWGG 2 cut(s) 908, 1089
MvnI CGCG 2 cut(s) 216, 376
MwoI GCNNNNNNNGC 1 cut(s) 1076
NdeII GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
NlaIII CATG 2 cut(s) 515, 1018
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 293, 1045
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PfoI TCCNGGA 2 cut(s) 906, 1087
PkrI GCNGC 4 cut(s) 163, 577, 724, 1078
Ple19I CGATCG 1 cut(s) 55
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
Psp124BI GAGCTC 1 cut(s) 309
Psp1406I AACGTT 1 cut(s) 1021
Psp6I CCWGG 2 cut(s) 906, 1087
PspGI CCWGG 2 cut(s) 906, 1087
PspN4I GGNNCC 1 cut(s) 438
PspPI GGNCC 3 cut(s) 57, 122, 558
PsuI RGATCY 3 cut(s) 231, 457, 961
PvuI CGATCG 1 cut(s) 55
RsaI GTAC 5 cut(s) 87, 138, 396, 756, 1098
RsaNI GTAC 5 cut(s) 86, 137, 395, 755, 1097
RseI CAYNNNNRTG 1 cut(s) 750
SacI GAGCTC 1 cut(s) 309
SaqAI TTAA 5 cut(s) 14, 155, 383, 654, 789
SatI GCNGC 4 cut(s) 162, 576, 723, 1077
Sau3AI GATC 8 cut(s) 52, 231, 298, 406, 457, 466, 514, 961
Sau96I GGNCC 3 cut(s) 57, 122, 558
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 2 cut(s) 908, 1089
SduI GDGCHC 3 cut(s) 309, 441, 993
SfaNI GCATC 2 cut(s) 607, 754
SinI GGWCC 3 cut(s) 57, 122, 558
SmiMI CAYNNNNRTG 1 cut(s) 750
Sse9I AATT 8 cut(s) 205, 580, 668, 800, 837, 849, 1081, 1105
SsiI CCGC 7 cut(s) 120, 376, 561, 576, 723, 782, 1077
SspI AATATT 1 cut(s) 144
SspMI CTAG 3 cut(s) 404, 1067, 1071
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 906, 1087
TaiI ACGT 1 cut(s) 1024
TaqI TCGA 5 cut(s) 51, 187, 338, 537, 842
TasI AATT 8 cut(s) 205, 580, 668, 800, 837, 849, 1081, 1105
TatI WGTACW 2 cut(s) 394, 754
TauI GCSGC 3 cut(s) 578, 725, 1079
Tru1I TTAA 5 cut(s) 14, 155, 383, 654, 789
Tru9I TTAA 5 cut(s) 14, 155, 383, 654, 789
TseFI GTSAC 2 cut(s) 293, 1045
TseI GCWGC 1 cut(s) 161
Tsp45I GTSAC 2 cut(s) 293, 1045
TspDTI ATGAA 3 cut(s) 190, 274, 813
TspGWI ACGGA 1 cut(s) 450
VpaK11BI GGWCC 3 cut(s) 57, 122, 558
XagI CCTNNNNNAGG 2 cut(s) 79, 129
XapI RAATTY 2 cut(s) 800, 837
XspI CTAG 3 cut(s) 404, 1067, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.