RLG00000028594

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010601
Physical Location & Seq
Forward (+)
24816788 .. 24820486
3699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028594

Sequence Viewer

Length: 1119 bp
ATGGCCGGTGCTTCTTCATCGTCTGGAGGCAGTCGCAAATACGACGTTTTCATCAATTTCAGAGGCGAAGACACACGTAGGTGTTTCGTCTGCCATCTTTACCAAGCTCTGAAACAGAGAGCACTCGACCCCTTCATTGATTCCGAAGAGCTTAGAAAAGGCGACGACCTTTCCGAGCTATTCAAAGCCATTCAAGACTCGAGGCTTTCGATTGTAGTTTTCTCTAAAGACTACGCATCTTCTAAATGGTGCTTAAAAGAACTGGTCCAGATCTTGGAGTGTATGGATAGGCGGAGTCAGATTGTGGTGCCCATTTTCTATGAAGTAGATCCCTCCCATGTTCGCAACCTTAAGGAAAGTTTTGCACAAGCTTTTGCAAAACATGAAAGCGGTTCCAATGTCGACAAAGAAGAGTTGGAGAGCTGGAAGTCCACTTTAGAGAGAGCAACCAATTTATCTGGCTGGGATTCCAAAAATTATCGTCACTCAAAGAATCTTATCAAAACCCCCGACTTCACTGAGGCAACAAATCTTGAGAAGCTGAAGCTTAAAGAATGTACAAGTTTATTGGAGGTTCACCCATCCATTTCAGCTCTTAAAAGCCTCGTCGAGTTGATCCTACGAGGCTGCTCAAATCTCAAGGAGTTTCCAAATATATCCGGAAATATGAAGAAGCTAAGAGAACTTTACTTAGATGGGACTGCAATTACAGAAGTGCCCTCGTCAATTAATGATCTTTTCGGGCTGCAGATTTTGAGCCTCGCTGGCTGCTCGAAACTTGAGGAGTTTCCAGAGATTTCAGAAGGAATGAAGAAGCTATGGAAGCTTAAATTAGACGGGACTGCAATTAAAAAACTACCCTCATCTATTAATAATCTTACGGGGCTTTATCGGTTAAGTTTGAGATACTGCAGAAGCCTTGTCTGTCTTCCGGACAATATCTGCAGTTTGAAGCAACTTTCTCAACTCGATCTGACTGGGTGCTCTAAGCTTTCAAAGTTGCCTGAGAATTTTGGGAATTTGGGACCCTTCTGGTTGGTGAACAATACCAATATATTTATAGATATTGTGAAAAGTGCTACTGATAATCAGGTTCATGCCCTCTCTCACAAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

373

Amino Acids

41.98

Weight (kDa)

8.38

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 168 1.8e-47 TIR domain
TIR_2 PF13676 16 - 111 7.2e-16 TIR domain
LRR_14 PF23598 191 - 302 1.5e-11 Leucine-rich repeat region
LRR_13 PF23286 248 - 333 2.5e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 275 - 341 2.3e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 307
AccB7I CCANNNNNTGG 1 cut(s) 274
AccI GTMKAC 1 cut(s) 402
AccIII TCCGGA 2 cut(s) 659, 931
AciI CCGC 2 cut(s) 292, 390
AclWI GGATC 2 cut(s) 323, 610
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 1009, 1018
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 1 cut(s) 274
AflII CTTAAG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 4 cut(s) 184, 194, 952, 996
AleI CACNNNNGTG 1 cut(s) 79
Alw21I GWGCWC 2 cut(s) 124, 986
AlwI GGATC 2 cut(s) 323, 610
Ama87I CYCGRG 1 cut(s) 199
Aor13HI TCCGGA 2 cut(s) 659, 931
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 627, 745, 768
ApoI RAATTY 2 cut(s) 1009, 1018
AseI ATTAAT 2 cut(s) 729, 870
AspS9I GGNCC 2 cut(s) 265, 1025
AsuHPI GGTGA 2 cut(s) 569, 1051
AvaI CYCGRG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 265, 1025
BaeGI GKGCMC 2 cut(s) 312, 720
BanI GGYRCC 1 cut(s) 307
BbsI GAAGAC 2 cut(s) 75, 920
Bbv12I GWGCWC 2 cut(s) 124, 986
BbvI GCAGC 3 cut(s) 614, 732, 755
BccI CCATC 3 cut(s) 102, 589, 689
BfmI CTRYAG 3 cut(s) 746, 910, 943
BfrI CTTAAG 1 cut(s) 350
BglI GCCNNNNNGGC 1 cut(s) 765
BglII AGATCT 1 cut(s) 270
BisI GCNGC 3 cut(s) 628, 746, 769
BlsI GCNGC 3 cut(s) 629, 747, 770
Bme18I GGWCC 2 cut(s) 265, 1025
BmeT110I CYCGRG 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 265, 1025
BmiI GGNNCC 4 cut(s) 309, 394, 1026, 1027
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 1 cut(s) 245
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 2 cut(s) 75, 920
BpmI CTGGAG 1 cut(s) 45
BpuEI CTTGAG 3 cut(s) 554, 623, 800
BsaAI YACGTR 1 cut(s) 77
BsaWI WCCGGW 2 cut(s) 659, 931
Bsc4I CCNNNNNNNGG 1 cut(s) 274
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 2 cut(s) 267, 982
BseAI TCCGGA 2 cut(s) 659, 931
BseGI GGATG 1 cut(s) 581
BseLI CCNNNNNNNGG 1 cut(s) 274
BseMII CTCAG 2 cut(s) 510, 996
BseNI ACTGG 2 cut(s) 267, 982
BseRI GAGGAG 1 cut(s) 797
BseSI GKGCMC 2 cut(s) 312, 720
BseXI GCAGC 3 cut(s) 614, 732, 755
BseYI CCCAGC 1 cut(s) 462
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 307
BsiHKAI GWGCWC 2 cut(s) 124, 986
BsiHKCI CYCGRG 1 cut(s) 199
BsiSI CCGG 3 cut(s) 6, 660, 932
BslFI GGGAC 3 cut(s) 712, 853, 1038
BslI CCNNNNNNNGG 1 cut(s) 274
BsmFI GGGAC 3 cut(s) 712, 853, 1038
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 4 cut(s) 124, 312, 720, 986
Bsp13I TCCGGA 2 cut(s) 659, 931
Bsp1407I TGTACA 1 cut(s) 557
Bsp143I GATC 5 cut(s) 270, 328, 615, 733, 970
BspACI CCGC 2 cut(s) 292, 390
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 511, 997
BspEI TCCGGA 2 cut(s) 659, 931
BspLI GGNNCC 4 cut(s) 309, 394, 1026, 1027
BspMAI CTGCAG 3 cut(s) 750, 914, 947
BspPI GGATC 2 cut(s) 323, 610
BspQI GCTCTTC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 307
BspTI CTTAAG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 557
BsrI ACTGG 2 cut(s) 267, 982
BssAI RCCGGY 1 cut(s) 5
BssMI GATC 5 cut(s) 270, 328, 615, 733, 970
Bst6I CTCTTC 2 cut(s) 141, 405
BstAFI CTTAAG 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 557
BstBAI YACGTR 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 766
BstDEI CTNAG 6 cut(s) 152, 519, 677, 691, 987, 1005
BstF5I GGATG 1 cut(s) 581
BstKTI GATC 5 cut(s) 273, 331, 618, 736, 973
BstMBI GATC 5 cut(s) 270, 328, 615, 733, 970
BstMWI GCNNNNNNNGC 2 cut(s) 765, 823
BstSFI CTRYAG 3 cut(s) 746, 910, 943
BstSLI GKGCMC 2 cut(s) 312, 720
BstV1I GCAGC 3 cut(s) 614, 732, 755
BstV2I GAAGAC 2 cut(s) 75, 920
BstX2I RGATCY 2 cut(s) 270, 328
BstYI RGATCY 2 cut(s) 270, 328
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 581
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 766
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 2 cut(s) 265, 1025
Csp6I GTAC 1 cut(s) 558
CviAII CATG 3 cut(s) 338, 383, 1097
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 6 cut(s) 152, 519, 677, 691, 987, 1005
DpnI GATC 5 cut(s) 272, 330, 617, 735, 972
DpnII GATC 5 cut(s) 270, 328, 615, 733, 970
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 141, 405
EarI CTCTTC 2 cut(s) 141, 405
EciI GGCGGA 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 265, 1025
Eco57I CTGAAG 1 cut(s) 563
Eco88I CYCGRG 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 1025
FaeI CATG 3 cut(s) 341, 386, 1100
FaqI GGGAC 3 cut(s) 712, 853, 1038
FatI CATG 3 cut(s) 337, 382, 1096
FblI GTMKAC 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 628, 746, 769
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 3 cut(s) 628, 746, 769
GluI GCNGC 3 cut(s) 628, 746, 769
GsaI CCCAGC 1 cut(s) 466
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 6, 660, 932
Hin1II CATG 3 cut(s) 341, 386, 1100
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 4 cut(s) 369, 545, 824, 989
HinfI GANTC 5 cut(s) 140, 197, 295, 467, 493
HpaII CCGG 3 cut(s) 6, 660, 932
HphI GGTGA 2 cut(s) 569, 1051
Hpy166II GTNNAC 4 cut(s) 403, 432, 577, 1042
Hpy188I TCNGA 7 cut(s) 62, 111, 145, 175, 300, 802, 975
Hpy188III TCNNGA 7 cut(s) 24, 194, 268, 533, 660, 791, 932
Hpy8I GTNNAC 4 cut(s) 403, 432, 577, 1042
Hpy99I CGWCG 3 cut(s) 47, 167, 611
HpyAV CCTTC 3 cut(s) 142, 797, 1039
HpyCH4IV ACGT 2 cut(s) 45, 76
HpyCH4V TGCA 7 cut(s) 365, 377, 704, 748, 845, 912, 945
HpyF10VI GCNNNNNNNGC 2 cut(s) 765, 823
HpyF3I CTNAG 6 cut(s) 152, 519, 677, 691, 987, 1005
HpySE526I ACGT 2 cut(s) 45, 76
Hsp92II CATG 3 cut(s) 341, 386, 1100
KflI GGGWCCC 1 cut(s) 1025
Kpn2I TCCGGA 2 cut(s) 659, 931
Kzo9I GATC 5 cut(s) 270, 328, 615, 733, 970
LguI GCTCTTC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 614, 732, 755
LweI GCATC 1 cut(s) 245
MaeII ACGT 2 cut(s) 45, 76
MaeIII GTNAC 1 cut(s) 482
MalI GATC 5 cut(s) 272, 330, 617, 735, 972
MboI GATC 5 cut(s) 270, 328, 615, 733, 970
MboII GAAGA 8 cut(s) 6, 80, 158, 231, 422, 682, 823, 920
MflI RGATCY 2 cut(s) 270, 328
MhlI GDGCHC 4 cut(s) 124, 312, 720, 986
MluCI AATT 9 cut(s) 55, 451, 475, 705, 726, 830, 846, 1009, 1018
MlyI GAGTC 2 cut(s) 191, 304
MmeI TCCRAC 1 cut(s) 396
MroI TCCGGA 2 cut(s) 659, 931
MseI TTAA 9 cut(s) 254, 351, 549, 597, 729, 828, 849, 870, 896
MslI CAYNNNNRTG 1 cut(s) 79
MspCI CTTAAG 1 cut(s) 350
MspI CCGG 3 cut(s) 6, 660, 932
MwoI GCNNNNNNNGC 2 cut(s) 765, 823
NdeII GATC 5 cut(s) 270, 328, 615, 733, 970
NlaIII CATG 3 cut(s) 341, 386, 1100
NlaIV GGNNCC 4 cut(s) 309, 394, 1026, 1027
NmuCI GTSAC 1 cut(s) 482
OliI CACNNNNGTG 1 cut(s) 79
PaeR7I CTCGAG 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 206
PfeI GAWTC 3 cut(s) 140, 467, 493
PflMI CCANNNNNTGG 1 cut(s) 274
PkrI GCNGC 3 cut(s) 629, 747, 770
PleI GAGTC 2 cut(s) 191, 303
PpsI GAGTC 2 cut(s) 191, 303
Ppu21I YACGTR 1 cut(s) 77
PpuMI RGGWCCY 1 cut(s) 1025
PshBI ATTAAT 2 cut(s) 729, 870
Psp5II RGGWCCY 1 cut(s) 1025
PspFI CCCAGC 1 cut(s) 462
PspN4I GGNNCC 4 cut(s) 309, 394, 1026, 1027
PspPI GGNCC 2 cut(s) 265, 1025
PspPPI RGGWCCY 1 cut(s) 1025
PspXI VCTCGAGB 1 cut(s) 199
PstI CTGCAG 3 cut(s) 750, 914, 947
PsuI RGATCY 2 cut(s) 270, 328
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
RseI CAYNNNNRTG 1 cut(s) 79
SalI GTCGAC 1 cut(s) 401
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 9 cut(s) 254, 351, 549, 597, 729, 828, 849, 870, 896
SatI GCNGC 3 cut(s) 628, 746, 769
Sau3AI GATC 5 cut(s) 270, 328, 615, 733, 970
Sau96I GGNCC 2 cut(s) 265, 1025
SchI GAGTC 2 cut(s) 191, 304
SduI GDGCHC 4 cut(s) 124, 312, 720, 986
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 3 cut(s) 746, 910, 943
Sfr274I CTCGAG 1 cut(s) 199
SinI GGWCC 2 cut(s) 265, 1025
SlaI CTCGAG 1 cut(s) 199
SmiMI CAYNNNNRTG 1 cut(s) 79
SmlI CTYRAG 5 cut(s) 199, 350, 533, 638, 779
SmoI CTYRAG 5 cut(s) 199, 350, 533, 638, 779
Sse9I AATT 9 cut(s) 55, 451, 475, 705, 726, 830, 846, 1009, 1018
SsiI CCGC 2 cut(s) 292, 390
TaiI ACGT 2 cut(s) 48, 79
TaqI TCGA 7 cut(s) 126, 200, 209, 402, 609, 773, 969
TasI AATT 9 cut(s) 55, 451, 475, 705, 726, 830, 846, 1009, 1018
TatI WGTACW 1 cut(s) 557
TfiI GAWTC 3 cut(s) 140, 467, 493
Tru1I TTAA 9 cut(s) 254, 351, 549, 597, 729, 828, 849, 870, 896
Tru9I TTAA 9 cut(s) 254, 351, 549, 597, 729, 828, 849, 870, 896
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 482
TseI GCWGC 3 cut(s) 627, 745, 768
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 8 cut(s) 6, 40, 124, 336, 399, 683, 824, 1085
TspRI CASTG 1 cut(s) 523
Van91I CCANNNNNTGG 1 cut(s) 274
Vha464I CTTAAG 1 cut(s) 350
VpaK11BI GGWCC 2 cut(s) 265, 1025
VspI ATTAAT 2 cut(s) 729, 870
XapI RAATTY 2 cut(s) 1009, 1018
XhoI CTCGAG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.