MD07G1015200.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010601
Physical Location & Seq
Forward (+)
1365784 .. 1367331
1548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1015200.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGGCTGCTTCTTCTTCTTCTGACGTTCGTTGGAAATACGACGTGTTCATCAATTTCAGAGGGGAAGACACTCACACCGGCTTCATCAGCCATCTCTACAGAGCTCTGCTGCAGAAACCGATCAACACCTTCATTGATGCCGAAGAGCTCAGAAAAGGCGACCGCCTTTGCGAGGTCCGGACAGGGATTCAAGAGTCAAGGCTTTCGCTTGTAGTTTTCTCTCAAAACTATGCTCTTTCCACTTGCTGCTTGAAAGAACTCGTGGAAATCTTGGAACGCAGCTATACTAAGAACCAGATTGTGGTCCCCATTTTCTACCAAGTTGATCCGGCTACCGTTCGTAAACTCAAGGGAACTTTCGCCAAAGCTTTTGTTCAGCATGATCACGATTCTAACCTCTATTCTAAATCCAAAATGCTAGAGGTTGAGAGCTGGAGATCCGCTCTTACAACGGCCACCAATATTTCCGGCTTGGGGGATCCGCGAAACTATAAGAATGAAGCAGAGCTTATTGAGAAAATTGTAGAAGATGTTTATACGAAATTGATGCAATTCTCATCAACATCAAGCAAATATGACGTGTTCATCAATTTCAGAGAGGAAGACATTGACAAAGGCTTCATCAGCCATCTCGAAGGAGCCCTGCTGCAGAAACAGATCAACACCTTCATTGATGCTGAGAAGCTCCGAAAAGGCGACCGCCTTCCCGAGCTCCTGACGGTGATTCGAGAGTCAAGTCTTTCGCTTGTAGTTTTCTCTCGAAACTATGCTCGTTCCACTTGCTGCTTGAAAGAACTCATGGAAATCTTGGAATGCAGAGATACTAAGAACCAGATTGTGGTCCCCATTTTCTACCAAGTTGATCAGGCTAACGTTCGTGAAGTCGAGGGAACTTTCGCCAAAGATTTGTCTCAGCATAAGCACGATTCTAACTCCAAAATGTTAGAGGTTGGGAGATGGAGGGCTGGTTTGGTATTGCTGTGCTTTGAAAAAAAGCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

335

Amino Acids

38.44

Weight (kDa)

7.05

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 188 1.8e-45 TIR domain
TIR_2 PF13676 15 - 111 6e-11 TIR domain
TIR PF01582 191 - 324 1.2e-36 TIR domain
TIR_2 PF13676 194 - 306 1.1e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 301, 838
AccBSI CCGCTC 1 cut(s) 443
AccII CGCG 1 cut(s) 484
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 4 cut(s) 163, 441, 482, 700
AclI AACGTT 1 cut(s) 873
AclWI GGATC 4 cut(s) 320, 432, 473, 486
AcoI YGGCCR 1 cut(s) 453
AfiI CCNNNNNNNGG 4 cut(s) 172, 301, 474, 838
AflIII ACRYGT 2 cut(s) 42, 579
AgsI TTSAA 4 cut(s) 191, 253, 790, 989
AjiI CACGTC 2 cut(s) 43, 580
AluBI AGCT 9 cut(s) 104, 148, 282, 368, 432, 508, 685, 712, 997
AluI AGCT 9 cut(s) 104, 148, 282, 368, 432, 508, 685, 712, 997
Alw21I GWGCWC 3 cut(s) 106, 150, 714
Alw26I GTCTC 1 cut(s) 915
AlwI GGATC 4 cut(s) 320, 432, 473, 486
Ama87I CYCGRG 1 cut(s) 707
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 1 cut(s) 453
ApeKI GCWGC 7 cut(s) 5, 109, 246, 279, 646, 783, 997
AspS9I GGNCC 3 cut(s) 175, 304, 841
AsuHPI GGTGA 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 3 cut(s) 175, 304, 841
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 4 cut(s) 106, 150, 643, 714
BauI CACGAG 1 cut(s) 260
BbsI GAAGAC 2 cut(s) 72, 609
Bbv12I GWGCWC 3 cut(s) 106, 150, 714
BbvI GCAGC 6 cut(s) 96, 233, 291, 633, 770, 984
BccI CCATC 3 cut(s) 99, 636, 951
BceAI ACGGC 1 cut(s) 468
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BclI TGATCA 2 cut(s) 382, 862
BcoDI GTCTC 1 cut(s) 915
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 3 cut(s) 97, 110, 647
BisI GCNGC 7 cut(s) 6, 110, 247, 280, 647, 784, 998
BlsI GCNGC 7 cut(s) 7, 111, 248, 281, 648, 785, 999
Bme18I GGWCC 3 cut(s) 175, 304, 841
BmeT110I CYCGRG 1 cut(s) 707
BmgBI CACGTC 2 cut(s) 43, 580
BmgT120I GGNCC 3 cut(s) 175, 304, 841
BmiI GGNNCC 4 cut(s) 306, 480, 640, 843
BmsI GCATC 3 cut(s) 127, 537, 664
BpiI GAAGAC 2 cut(s) 72, 609
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpmI CTGGAG 1 cut(s) 454
BpuEI CTTGAG 1 cut(s) 332
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 952, 982
Bsc4I CCNNNNNNNGG 4 cut(s) 172, 301, 474, 838
Bse118I RCCGGY 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 177
BseLI CCNNNNNNNGG 4 cut(s) 172, 301, 474, 838
BseMII CTCAG 3 cut(s) 163, 669, 926
BseXI GCAGC 6 cut(s) 96, 233, 291, 633, 770, 984
Bsh1236I CGCG 1 cut(s) 484
Bsh1285I CGRYCG 2 cut(s) 163, 700
BshFI GGCC 1 cut(s) 455
BsiEI CGRYCG 2 cut(s) 163, 700
BsiHKAI GWGCWC 3 cut(s) 106, 150, 714
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 4 cut(s) 78, 178, 329, 468
BslFI GGGAC 2 cut(s) 290, 827
BslI CCNNNNNNNGG 4 cut(s) 172, 301, 474, 838
BsmAI GTCTC 1 cut(s) 915
BsmFI GGGAC 2 cut(s) 290, 827
BsmI GAATGC 1 cut(s) 818
BsnI GGCC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 707
Bsp1286I GDGCHC 4 cut(s) 106, 150, 643, 714
Bsp13I TCCGGA 1 cut(s) 177
Bsp143I GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
BspACI CCGC 4 cut(s) 163, 441, 482, 700
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 3 cut(s) 162, 670, 925
BspEI TCCGGA 1 cut(s) 177
BspFNI CGCG 1 cut(s) 484
BspLI GGNNCC 4 cut(s) 306, 480, 640, 843
BspMAI CTGCAG 2 cut(s) 114, 651
BspPI GGATC 4 cut(s) 320, 432, 473, 486
BspQI GCTCTTC 1 cut(s) 138
BsrBI CCGCTC 1 cut(s) 443
BsrFI RCCGGY 1 cut(s) 77
BssAI RCCGGY 1 cut(s) 77
BssMI GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
BssSI CACGAG 1 cut(s) 260
Bst2BI CACGAG 1 cut(s) 260
Bst4CI ACNGT 2 cut(s) 337, 721
Bst6I CTCTTC 1 cut(s) 138
BstDEI CTNAG 5 cut(s) 149, 288, 678, 825, 912
BstENI CCTNNNNNAGG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 484
BstKTI GATC 7 cut(s) 123, 328, 385, 440, 481, 660, 865
BstMAI GTCTC 1 cut(s) 915
BstMBI GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
BstMCI CGRYCG 2 cut(s) 163, 700
BstMWI GCNNNNNNNGC 2 cut(s) 87, 624
BstSFI CTRYAG 3 cut(s) 97, 110, 647
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 6 cut(s) 96, 233, 291, 633, 770, 984
BstV2I GAAGAC 2 cut(s) 72, 609
BstX2I RGATCY 2 cut(s) 437, 478
BstYI RGATCY 2 cut(s) 437, 478
BsuRI GGCC 1 cut(s) 455
BtrI CACGTC 2 cut(s) 43, 580
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 3 cut(s) 175, 304, 841
CviAII CATG 2 cut(s) 380, 799
DdeI CTNAG 5 cut(s) 149, 288, 678, 825, 912
DpnI GATC 7 cut(s) 122, 327, 384, 439, 480, 659, 864
DpnII GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
EaeI YGGCCR 1 cut(s) 453
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Ecl136II GAGCTC 3 cut(s) 104, 148, 712
Eco24I GRGCYC 4 cut(s) 106, 150, 643, 714
Eco47I GGWCC 3 cut(s) 175, 304, 841
Eco53kI GAGCTC 3 cut(s) 104, 148, 712
Eco88I CYCGRG 1 cut(s) 707
EcoICRI GAGCTC 3 cut(s) 104, 148, 712
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoT38I GRGCYC 4 cut(s) 106, 150, 643, 714
FaeI CATG 2 cut(s) 383, 802
FaiI YATR 9 cut(s) 231, 285, 381, 492, 537, 576, 768, 800, 918
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 2 cut(s) 290, 827
FatI CATG 2 cut(s) 379, 798
FbaI TGATCA 2 cut(s) 382, 862
Fnu4HI GCNGC 7 cut(s) 6, 110, 247, 280, 647, 784, 998
FriOI GRGCYC 4 cut(s) 106, 150, 643, 714
Fsp4HI GCNGC 7 cut(s) 6, 110, 247, 280, 647, 784, 998
FspBI CTAG 1 cut(s) 419
GluI GCNGC 7 cut(s) 6, 110, 247, 280, 647, 784, 998
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 1 cut(s) 455
HapII CCGG 4 cut(s) 78, 178, 329, 468
Hin1II CATG 2 cut(s) 383, 802
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 6 cut(s) 187, 194, 389, 724, 731, 926
HpaII CCGG 4 cut(s) 78, 178, 329, 468
HphI GGTGA 1 cut(s) 733
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 5 cut(s) 22, 59, 152, 596, 689
Hpy188III TCNNGA 9 cut(s) 178, 191, 386, 632, 707, 715, 728, 759, 878
Hpy8I GTNNAC 1 cut(s) 344
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 4 cut(s) 139, 629, 676, 713
HpyCH4III ACNGT 2 cut(s) 337, 721
HpyCH4IV ACGT 4 cut(s) 24, 42, 579, 873
HpyCH4V TGCA 4 cut(s) 112, 550, 649, 816
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 624
HpyF3I CTNAG 5 cut(s) 149, 288, 678, 825, 912
HpySE526I ACGT 4 cut(s) 24, 42, 579, 873
Hsp92II CATG 2 cut(s) 383, 802
Kpn2I TCCGGA 1 cut(s) 177
Ksp22I TGATCA 2 cut(s) 382, 862
Kzo9I GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 3 cut(s) 638, 690, 717
Lsp1109I GCAGC 6 cut(s) 96, 233, 291, 633, 770, 984
LweI GCATC 3 cut(s) 127, 537, 664
MaeI CTAG 1 cut(s) 419
MaeII ACGT 4 cut(s) 24, 42, 579, 873
MalI GATC 7 cut(s) 122, 327, 384, 439, 480, 659, 864
MbiI CCGCTC 1 cut(s) 443
MboI GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
MboII GAAGA 7 cut(s) 3, 6, 9, 77, 155, 539, 614
MflI RGATCY 2 cut(s) 437, 478
MhlI GDGCHC 4 cut(s) 106, 150, 643, 714
MluCI AATT 5 cut(s) 52, 519, 542, 551, 589
MlyI GAGTC 2 cut(s) 203, 740
MmeI TCCRAC 1 cut(s) 11
MnlI CCTC 8 cut(s) 53, 166, 407, 415, 592, 880, 940, 954
MroI TCCGGA 1 cut(s) 177
MspI CCGG 4 cut(s) 78, 178, 329, 468
Mva1269I GAATGC 1 cut(s) 818
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 2 cut(s) 87, 624
NdeII GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
NlaIII CATG 2 cut(s) 383, 802
NlaIV GGNNCC 4 cut(s) 306, 480, 640, 843
PciSI GCTCTTC 1 cut(s) 138
PctI GAATGC 1 cut(s) 818
PfeI GAWTC 4 cut(s) 187, 389, 724, 926
PflMI CCANNNNNTGG 2 cut(s) 301, 838
PkrI GCNGC 7 cut(s) 7, 111, 248, 281, 648, 785, 999
PleI GAGTC 2 cut(s) 202, 739
PpsI GAGTC 2 cut(s) 202, 739
Psp124BI GAGCTC 3 cut(s) 106, 150, 714
Psp1406I AACGTT 1 cut(s) 873
PspN4I GGNNCC 4 cut(s) 306, 480, 640, 843
PspPI GGNCC 3 cut(s) 175, 304, 841
PstI CTGCAG 2 cut(s) 114, 651
PsuI RGATCY 2 cut(s) 437, 478
SacI GAGCTC 3 cut(s) 106, 150, 714
SapI GCTCTTC 1 cut(s) 138
SatI GCNGC 7 cut(s) 6, 110, 247, 280, 647, 784, 998
Sau3AI GATC 7 cut(s) 120, 325, 382, 437, 478, 657, 862
Sau96I GGNCC 3 cut(s) 175, 304, 841
SchI GAGTC 2 cut(s) 203, 740
SduI GDGCHC 4 cut(s) 106, 150, 643, 714
SfaNI GCATC 3 cut(s) 127, 537, 664
SfcI CTRYAG 3 cut(s) 97, 110, 647
SinI GGWCC 3 cut(s) 175, 304, 841
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 5 cut(s) 52, 519, 542, 551, 589
SsiI CCGC 4 cut(s) 163, 441, 482, 700
SspI AATATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 419
SstI GAGCTC 3 cut(s) 106, 150, 714
TaaI ACNGT 2 cut(s) 337, 721
TaiI ACGT 4 cut(s) 27, 45, 582, 876
TaqI TCGA 4 cut(s) 633, 727, 760, 885
TasI AATT 5 cut(s) 52, 519, 542, 551, 589
TfiI GAWTC 4 cut(s) 187, 389, 724, 926
TseI GCWGC 7 cut(s) 5, 109, 246, 279, 646, 783, 997
TspDTI ATGAA 7 cut(s) 37, 73, 121, 513, 574, 610, 658
Van91I CCANNNNNTGG 2 cut(s) 301, 838
VpaK11BI GGWCC 3 cut(s) 175, 304, 841
XagI CCTNNNNNAGG 1 cut(s) 170
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.