RchiOBHm_Chr1g0375961

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0010601
Physical Location & Seq
Forward (+)
63241930 .. 63246213
4284 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59964

Sequence Viewer

Length: 537 bp
ATGATGGCTTCTTCTTCATCATCCAGCGGTCACCGGAAGTATGATGTCTTCCTAAGTTTTAGAGGAGAAGACACCCGAAAAATCTTCGTCGGCCATCTTTACAGAGCTCTGCAACAGAAAGCAATCAATACTTTCATTGATTCTGAAGAGCTGAGTAAAGGCAACAAGATTTCGGAGCTACTCACAGCTATTGAGGACTTGAGGATTTCGATTGCTGCCACTTCCACTTGGTGCTTACAAGAGCTCGTCAAAATCCTGGATTGCATGGATACGAAGAAGCAGATAGTGGTGCCCATCTTCTACGAAGTAGATCCCTCTCATCTACGGAAAATCAATGGAAAATTTGCAGAAGCTTTTGCCAAACATGGAGAAAATCCTAACCTCGACAAGGAAGAGTTGAAGAGCTGGAGATCCGCTCTAACTGCAACTGCGGATCTATCTGGCTGGGATTCGAGCAAATATGAGGATGATGGCAAGCTTATTGAGGAAATTGTTGAAAATACATTAAAAAAACTGATTCACACTTCGTCCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

178

Amino Acids

20.11

Weight (kDa)

5.98

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 176 2.2e-44 TIR domain
TIR_2 PF13676 16 - 111 1.4e-08 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 289
AccBSI CCGCTC 1 cut(s) 416
AciI CCGC 3 cut(s) 27, 414, 431
AclWI GGATC 3 cut(s) 305, 405, 441
AcoI YGGCCR 1 cut(s) 91
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 165
AdeI CACNNNGTG 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 2 cut(s) 400, 497
AjnI CCWGG 1 cut(s) 255
AluBI AGCT 8 cut(s) 107, 151, 178, 188, 244, 353, 405, 478
AluI AGCT 8 cut(s) 107, 151, 178, 188, 244, 353, 405, 478
Alw21I GWGCWC 2 cut(s) 109, 246
AlwI GGATC 3 cut(s) 305, 405, 441
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 341
AsuHPI GGTGA 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 2 cut(s) 109, 246
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
BbsI GAAGAC 2 cut(s) 40, 75
Bbv12I GWGCWC 2 cut(s) 109, 246
BbvI GCAGC 1 cut(s) 202
BccI CCATC 3 cut(s) 102, 302, 464
BciT130I CCWGG 1 cut(s) 257
BciVI GTATCC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 262
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 257
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 1 cut(s) 257
BpiI GAAGAC 2 cut(s) 40, 75
BplI GAGNNNNNCTC 2 cut(s) 400, 432
BpmI CTGGAG 1 cut(s) 427
BpuEI CTTGAG 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 388
BseBI CCWGG 1 cut(s) 257
BseGI GGATG 2 cut(s) 20, 472
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 294
BseXI GCAGC 1 cut(s) 202
BseYI CCCAGC 1 cut(s) 444
BshFI GGCC 1 cut(s) 93
BshNI GGYRCC 1 cut(s) 289
BsiHKAI GWGCWC 2 cut(s) 109, 246
BsiSI CCGG 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 388
BsnI GGCC 1 cut(s) 93
Bsp1286I GDGCHC 3 cut(s) 109, 246, 294
Bsp143I GATC 3 cut(s) 310, 410, 433
BspACI CCGC 3 cut(s) 27, 414, 431
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 3 cut(s) 305, 405, 441
BspQI GCTCTTC 2 cut(s) 141, 395
BspT107I GGYRCC 1 cut(s) 289
BsrBI CCGCTC 1 cut(s) 416
BssMI GATC 3 cut(s) 310, 410, 433
Bst2UI CCWGG 1 cut(s) 257
Bst6I CTCTTC 3 cut(s) 141, 387, 395
BstC8I GCNNGC 1 cut(s) 476
BstDEI CTNAG 2 cut(s) 53, 152
BstEII GGTNACC 1 cut(s) 29
BstENI CCTNNNNNAGG 1 cut(s) 386
BstF5I GGATG 2 cut(s) 20, 472
BstKTI GATC 3 cut(s) 313, 413, 436
BstMBI GATC 3 cut(s) 310, 410, 433
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstNI CCWGG 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 255
BstSLI GKGCMC 1 cut(s) 294
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 40, 75
BstX2I RGATCY 3 cut(s) 310, 410, 433
BstYI RGATCY 3 cut(s) 310, 410, 433
BsuI GTATCC 1 cut(s) 262
BsuRI GGCC 1 cut(s) 93
BtsCI GGATG 2 cut(s) 20, 472
Cac8I GCNNGC 1 cut(s) 476
CviAII CATG 2 cut(s) 265, 365
DdeI CTNAG 2 cut(s) 53, 152
DpnI GATC 3 cut(s) 312, 412, 435
DpnII GATC 3 cut(s) 310, 410, 433
DraIII CACNNNGTG 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 91
Eam1104I CTCTTC 3 cut(s) 141, 387, 395
EarI CTCTTC 3 cut(s) 141, 387, 395
Ecl136II GAGCTC 2 cut(s) 107, 244
Eco24I GRGCYC 2 cut(s) 109, 246
Eco53kI GAGCTC 2 cut(s) 107, 244
Eco57I CTGAAG 1 cut(s) 165
Eco91I GGTNACC 1 cut(s) 29
EcoICRI GAGCTC 2 cut(s) 107, 244
EcoNI CCTNNNNNAGG 1 cut(s) 386
EcoO65I GGTNACC 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 255
EcoT38I GRGCYC 2 cut(s) 109, 246
FaeI CATG 2 cut(s) 268, 368
FaiI YATR 4 cut(s) 42, 266, 366, 462
FatI CATG 2 cut(s) 264, 364
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 7, 479
FriOI GRGCYC 2 cut(s) 109, 246
Fsp4HI GCNGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 216
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 1 cut(s) 427
HaeIII GGCC 1 cut(s) 93
HapII CCGG 1 cut(s) 34
Hin1II CATG 2 cut(s) 268, 368
HindIII AAGCTT 2 cut(s) 351, 476
HinfI GANTC 3 cut(s) 140, 449, 517
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 145, 175
Hpy99I CGWCG 1 cut(s) 92
HpyCH4V TGCA 4 cut(s) 112, 264, 347, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 2 cut(s) 53, 152
Hsp92II CATG 2 cut(s) 268, 368
Kzo9I GATC 3 cut(s) 310, 410, 433
LguI GCTCTTC 2 cut(s) 141, 395
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 7 cut(s) 37, 47, 242, 269, 391, 426, 430
Lsp1109I GCAGC 1 cut(s) 202
MaeIII GTNAC 1 cut(s) 29
MalI GATC 3 cut(s) 312, 412, 435
MbiI CCGCTC 1 cut(s) 416
MboI GATC 3 cut(s) 310, 410, 433
MflI RGATCY 3 cut(s) 310, 410, 433
MhlI GDGCHC 3 cut(s) 109, 246, 294
MluCI AATT 3 cut(s) 341, 489, 532
MnlI CCTC 7 cut(s) 56, 187, 195, 325, 392, 457, 478
MseI TTAA 2 cut(s) 506, 535
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 257
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 422
NdeII GATC 3 cut(s) 310, 410, 433
NlaIII CATG 2 cut(s) 268, 368
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 29
PciSI GCTCTTC 2 cut(s) 141, 395
PfeI GAWTC 3 cut(s) 140, 449, 517
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 1 cut(s) 217
Psp124BI GAGCTC 2 cut(s) 109, 246
Psp6I CCWGG 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 29
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 291
PsuI RGATCY 3 cut(s) 310, 410, 433
SacI GAGCTC 2 cut(s) 109, 246
SapI GCTCTTC 2 cut(s) 141, 395
SaqAI TTAA 2 cut(s) 506, 535
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 310, 410, 433
ScrFI CCNGG 1 cut(s) 257
SduI GDGCHC 3 cut(s) 109, 246, 294
SetI ASST 9 cut(s) 109, 153, 180, 190, 246, 355, 384, 407, 480
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 3 cut(s) 341, 489, 532
SsiI CCGC 3 cut(s) 27, 414, 431
SstI GAGCTC 2 cut(s) 109, 246
StyD4I CCNGG 1 cut(s) 255
TaqI TCGA 3 cut(s) 209, 384, 452
TasI AATT 3 cut(s) 341, 489, 532
TfiI GAWTC 3 cut(s) 140, 449, 517
Tru1I TTAA 2 cut(s) 506, 535
Tru9I TTAA 2 cut(s) 506, 535
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 2 cut(s) 6, 124
TspGWI ACGGA 1 cut(s) 340
XagI CCTNNNNNAGG 1 cut(s) 386
XapI RAATTY 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.