MD01G1200600.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007411
Physical Location & Seq
Reverse (-)
29727100 .. 29733141
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1200600.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGCGTTAAAGGTACCGGCGGTTGCCTTCACGTTTGTGGTACACACGCTCGGGGTTACGGCTTTGGCTTTGGTGTTGGTTTGGACCATTCATTTCAGAGGAGGTTTGGCATGGGAAGCTACCAACAAGAACCTCATCTTCAACCTCCATCCTGTTCTCATGTTACTTGGCATAATTATCATGGGAGGTGAAGCCATTGTTAGTCACAAATCACTGCCTTTCAATAAAGAGACGAAGAAAGTGATACACTTGGTTCTTCATACCCTCGCATTGGCTCTTGGGATCATTGGTATTTATCTTGTGTTCAAGAACCACAATGAGAGTGGAATTGCCAATCTGTACAGTTTGCACTCCTGGATTGGAATTGGGGTGATTGTTCTCTATGGAATTCAGTGGATATATGGGTTCCTGACCTTCTTCTACCCCGGAGGAAGTCCGACACTGAAGAGTGAGTCTATTCCATGGCATGTGGTGTTTGGAATCATCGTGTACTTACTGGCTGTTGGCAATGCTTCATTAGGGTTCTTAGAGAAGCTCACATTCCTCGAGAACTCGGGACTCGATAAGTTTGGGGCCGAGGCCTTTCTTGTCAACTTCACCGCAATCATCACAGTACTGTTCGGAATCTTCGTTATACTCTCGGTAATTTCTGAAGCTCCAGCTCCCGTTGAAGATGACAACATCCATTCTTCTATATATAAGGACTGA

Protein Analysis

236

Amino Acids

25.7

Weight (kDa)

6.54

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 49 - 181 4.8e-46 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AciI CCGC 2 cut(s) 20, 600
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 2 cut(s) 464, 672
AfaI GTAC 5 cut(s) 15, 42, 341, 491, 615
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 4 cut(s) 142, 223, 307, 671
AjnI CCWGG 1 cut(s) 353
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 4 cut(s) 119, 535, 656, 662
AluI AGCT 4 cut(s) 119, 535, 656, 662
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 3 cut(s) 50, 545, 553
AoxI GGCC 2 cut(s) 573, 579
ApoI RAATTY 1 cut(s) 387
Asp718I GGTACC 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 84, 573
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 200, 382, 589
AvaI CYCGRG 3 cut(s) 50, 545, 553
AvaII GGWCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 13
BccI CCATC 1 cut(s) 156
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 355
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 224
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 355, 426
Bme18I GGWCC 1 cut(s) 84
BmeT110I CYCGRG 3 cut(s) 50, 545, 553
BmgT120I GGNCC 2 cut(s) 84, 573
BmiI GGNNCC 3 cut(s) 15, 407, 574
BmrFI CCNGG 2 cut(s) 355, 426
BpmI CTGGAG 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 424, 461, 576
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 501
Bse3DI GCAATG 1 cut(s) 514
BseBI CCWGG 1 cut(s) 355
BseDI CCNNGG 3 cut(s) 424, 461, 576
BseGI GGATG 2 cut(s) 148, 681
BseLI CCNNNNNNNGG 1 cut(s) 271
BseMI GCAATG 1 cut(s) 514
BseNI ACTGG 1 cut(s) 501
BseRI GAGGAG 1 cut(s) 114
BshFI GGCC 2 cut(s) 575, 581
BshNI GGYRCC 1 cut(s) 13
BsiHKCI CYCGRG 3 cut(s) 50, 545, 553
BsiSI CCGG 2 cut(s) 17, 426
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 570
BsnI GGCC 2 cut(s) 575, 581
BsoBI CYCGRG 3 cut(s) 50, 545, 553
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 461
BspACI CCGC 2 cut(s) 20, 600
BspANI GGCC 2 cut(s) 575, 581
BspLI GGNNCC 3 cut(s) 15, 407, 574
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 1 cut(s) 501
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 424, 461, 576
BssMI GATC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 461
Bst2UI CCWGG 1 cut(s) 355
Bst4CI ACNGT 3 cut(s) 344, 613, 618
Bst6I CTCTTC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 526
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 148, 681
BstKTI GATC 1 cut(s) 285
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 353, 424
BsuRI GGCC 2 cut(s) 575, 581
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 2 cut(s) 148, 681
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 3 cut(s) 212, 398, 440
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 84, 573
Csp6I GTAC 5 cut(s) 14, 41, 340, 490, 614
CspCI CAANNNNNGTGG 2 cut(s) 304, 339
CviAII CATG 5 cut(s) 111, 160, 181, 462, 467
CviQI GTAC 5 cut(s) 14, 41, 340, 490, 614
DdeI CTNAG 1 cut(s) 526
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 461
Eco147I AGGCCT 1 cut(s) 581
Eco47I GGWCC 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 464, 672
Eco88I CYCGRG 3 cut(s) 50, 545, 553
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 5 cut(s) 114, 163, 184, 465, 470
FaqI GGGAC 1 cut(s) 570
FatI CATG 5 cut(s) 110, 159, 180, 461, 466
FokI GGATG 2 cut(s) 135, 668
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 2 cut(s) 575, 581
HapII CCGG 2 cut(s) 17, 426
Hin1II CATG 5 cut(s) 114, 163, 184, 465, 470
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 4 cut(s) 452, 480, 558, 624
HpaII CCGG 2 cut(s) 17, 426
HphI GGTGA 3 cut(s) 200, 382, 589
Hpy166II GTNNAC 3 cut(s) 43, 490, 592
Hpy188I TCNGA 4 cut(s) 98, 438, 623, 652
Hpy188III TCNNGA 4 cut(s) 307, 409, 547, 555
Hpy8I GTNNAC 3 cut(s) 43, 490, 592
HpyAV CCTTC 2 cut(s) 37, 424
HpyCH4III ACNGT 3 cut(s) 344, 613, 618
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 1 cut(s) 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 1 cut(s) 526
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 5 cut(s) 114, 163, 184, 465, 470
KpnI GGTACC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 282
LmnI GCTCC 2 cut(s) 661, 667
LpnPI CCDG 8 cut(s) 30, 165, 340, 367, 422, 439, 482, 672
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 55, 162, 203
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 8 cut(s) 130, 247, 248, 409, 457, 619, 681, 683
MluCI AATT 5 cut(s) 174, 327, 363, 387, 645
MlyI GAGTC 2 cut(s) 461, 552
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 9 cut(s) 92, 95, 143, 155, 179, 275, 422, 554, 571
MseI TTAA 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 2 cut(s) 17, 426
MspR9I CCNGG 2 cut(s) 355, 426
MvaI CCWGG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 426
NcoI CCATGG 1 cut(s) 461
NdeII GATC 1 cut(s) 282
NlaIII CATG 5 cut(s) 114, 163, 184, 465, 470
NlaIV GGNNCC 3 cut(s) 15, 407, 574
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 1 cut(s) 203
NspI RCATGY 1 cut(s) 470
OliI CACNNNNGTG 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 581
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PfeI GAWTC 2 cut(s) 480, 624
PfoI TCCNGGA 1 cut(s) 353
PleI GAGTC 2 cut(s) 460, 552
PpsI GAGTC 2 cut(s) 460, 552
Psp6I CCWGG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 15, 407, 574
PspPI GGNCC 2 cut(s) 84, 573
RsaI GTAC 5 cut(s) 15, 42, 341, 491, 615
RsaNI GTAC 5 cut(s) 14, 41, 340, 490, 614
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 1 cut(s) 8
Sau3AI GATC 1 cut(s) 282
Sau96I GGNCC 2 cut(s) 84, 573
ScaI AGTACT 1 cut(s) 615
SchI GAGTC 2 cut(s) 461, 552
ScrFI CCNGG 2 cut(s) 355, 426
Sfr274I CTCGAG 1 cut(s) 545
SinI GGWCC 1 cut(s) 84
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 5 cut(s) 174, 327, 363, 387, 645
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 2 cut(s) 20, 600
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 2 cut(s) 353, 424
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 3 cut(s) 344, 613, 618
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 546, 561
TasI AATT 5 cut(s) 174, 327, 363, 387, 645
TatI WGTACW 3 cut(s) 339, 489, 613
TfiI GAWTC 2 cut(s) 480, 624
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 3 cut(s) 219, 398, 447
TseFI GTSAC 1 cut(s) 203
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 3 cut(s) 80, 248, 504
TspRI CASTG 3 cut(s) 219, 398, 447
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 1 cut(s) 387
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 320
XhoI CTCGAG 1 cut(s) 545
ZrmI AGTACT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.