Rroxscaffold_4G00281360

Cytochrome b-561 / ferric reductase transmembrane domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007411
Physical Location & Seq
Forward (+)
3687159 .. 3690515
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281360.1

Sequence Viewer

Length: 657 bp
ATGCACACTATCGTATCGACGAGCTCTACAACTTTGATGAAGGAAGTTTTCCCAAATTCGATACGGGCGGTTTCATGGGAAGCCACCGACAAGAGCCTCATCTTCAACCTCCATCCTTTTCTCATGGTATTGGGCTTAGTTTTCCTTGGAGGAGAAGCCATTATCAGTTATAGATACCTTCCTTTGAGGAAAGATGTGAAGAAATTAGTGCACCTGGTTCTTCATGCTATCGCATTAGCACTCGGTATAATTGGCATTTATACTGCATTCAAGAACCACAATGAGACTGGGGTTGCCAATCTCTACAGTCTGCACTCCTGGATTGGCATTGGGGTTATTTGCCTCTATGGTATTCAGTGGATATATGGGTTCTTGGTATTCTTCTACCCAAAAGGAAGTCCAGGGCTGAGAAGTGACTCAGTTCCATGGCATGTGATTTTTGGGCTTCTCGTGTACATTTTGGTCGTCGGCAATGCTACCTTGGGGTTCTTGGAGAAGCTCACATTCCTTGAGAGCTCAGGAGTCGAAAAGTTTGGAAGTGAGGCATTTCTTGTCAACTTCACTGCTCTGGTTACACTCGTGTTTGGGGCCTTTGTGATATTCTCGGTCATTTCTCAGGGTCCTGTAGAAGAAGACCACAGCTACTCAGAAATATGA

Protein Analysis

218

Amino Acids

24.14

Weight (kDa)

6.43

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 37 - 169 2.2e-47 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 68
AcsI RAATTY 1 cut(s) 55
AfaI GTAC 1 cut(s) 455
AgsI TTSAA 2 cut(s) 106, 271
AjnI CCWGG 3 cut(s) 213, 317, 400
AluBI AGCT 4 cut(s) 24, 499, 516, 642
AluI AGCT 4 cut(s) 24, 499, 516, 642
Alw21I GWGCWC 3 cut(s) 26, 213, 518
Alw26I GTCTC 1 cut(s) 278
Alw44I GTGCAC 1 cut(s) 209
AoxI GGCC 1 cut(s) 588
ApaLI GTGCAC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 588, 620
AvaII GGWCC 1 cut(s) 620
BaeGI GKGCMC 1 cut(s) 213
BaeI ACNNNNGTAYC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 26, 518
BauI CACGAG 2 cut(s) 449, 578
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 3 cut(s) 26, 213, 518
BccI CCATC 1 cut(s) 120
BciT130I CCWGG 3 cut(s) 215, 319, 402
BcoDI GTCTC 1 cut(s) 278
BfmI CTRYAG 2 cut(s) 304, 624
Bme1390I CCNGG 3 cut(s) 215, 319, 402
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 2 cut(s) 588, 620
BmiI GGNNCC 2 cut(s) 589, 621
BmrFI CCNGG 3 cut(s) 215, 319, 402
BmrI ACTGGG 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 530
BsaJI CCNNGG 4 cut(s) 145, 401, 425, 480
Bse1I ACTGG 1 cut(s) 292
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 3 cut(s) 215, 319, 402
BseDI CCNNGG 4 cut(s) 145, 401, 425, 480
BseGI GGATG 1 cut(s) 112
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 4 cut(s) 398, 432, 531, 629
BseNI ACTGG 1 cut(s) 292
BseRI GAGGAG 1 cut(s) 165
BseSI GKGCMC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 296
BshFI GGCC 1 cut(s) 590
BsiHKAI GWGCWC 3 cut(s) 26, 213, 518
BsmAI GTCTC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 266
BsnI GGCC 1 cut(s) 590
Bsp1286I GDGCHC 3 cut(s) 26, 213, 518
Bsp1407I TGTACA 1 cut(s) 453
Bsp19I CCATGG 1 cut(s) 425
BspACI CCGC 1 cut(s) 68
BspANI GGCC 1 cut(s) 590
BspCNI CTCAG 4 cut(s) 399, 431, 530, 628
BspLI GGNNCC 2 cut(s) 589, 621
BsrDI GCAATG 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 453
BsrI ACTGG 1 cut(s) 292
BssECI CCNNGG 4 cut(s) 145, 401, 425, 480
BssSI CACGAG 2 cut(s) 449, 578
BssT1I CCWWGG 3 cut(s) 145, 425, 480
Bst2BI CACGAG 2 cut(s) 449, 578
Bst2UI CCWGG 3 cut(s) 215, 319, 402
Bst4CI ACNGT 1 cut(s) 308
BstAUI TGTACA 1 cut(s) 453
BstDEI CTNAG 6 cut(s) 136, 407, 418, 517, 615, 646
BstDSI CCRYGG 1 cut(s) 425
BstF5I GGATG 1 cut(s) 112
BstMAI GTCTC 1 cut(s) 278
BstNI CCWGG 3 cut(s) 215, 319, 402
BstNSI RCATGY 1 cut(s) 434
BstSCI CCNGG 3 cut(s) 213, 317, 400
BstSFI CTRYAG 2 cut(s) 304, 624
BstSLI GKGCMC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 639
BsuRI GGCC 1 cut(s) 590
BtgI CCRYGG 1 cut(s) 425
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 362, 561
Cfr13I GGNCC 2 cut(s) 588, 620
CsiI ACCWGGT 1 cut(s) 213
Csp6I GTAC 1 cut(s) 454
CviAII CATG 5 cut(s) 75, 124, 224, 426, 431
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 6 cut(s) 136, 407, 418, 517, 615, 646
Ecl136II GAGCTC 2 cut(s) 24, 516
Eco130I CCWWGG 3 cut(s) 145, 425, 480
Eco24I GRGCYC 2 cut(s) 26, 518
Eco47I GGWCC 1 cut(s) 620
Eco53kI GAGCTC 2 cut(s) 24, 516
EcoICRI GAGCTC 2 cut(s) 24, 516
EcoO109I RGGNCCY 2 cut(s) 588, 620
EcoRII CCWGG 3 cut(s) 213, 317, 400
EcoT14I CCWWGG 3 cut(s) 145, 425, 480
EcoT38I GRGCYC 2 cut(s) 26, 518
ErhI CCWWGG 3 cut(s) 145, 425, 480
FaeI CATG 5 cut(s) 78, 127, 227, 429, 434
FatI CATG 5 cut(s) 74, 123, 223, 425, 430
FokI GGATG 1 cut(s) 99
FriOI GRGCYC 2 cut(s) 26, 518
HaeIII GGCC 1 cut(s) 590
Hin1II CATG 5 cut(s) 78, 127, 227, 429, 434
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 2 cut(s) 416, 522
Hpy166II GTNNAC 3 cut(s) 211, 454, 556
Hpy188I TCNGA 1 cut(s) 649
Hpy188III TCNNGA 2 cut(s) 271, 519
Hpy8I GTNNAC 3 cut(s) 211, 454, 556
Hpy99I CGWCG 2 cut(s) 22, 470
HpyAV CCTTC 2 cut(s) 34, 188
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4V TGCA 4 cut(s) 4, 211, 266, 313
HpyF3I CTNAG 6 cut(s) 136, 407, 418, 517, 615, 646
Hsp92II CATG 5 cut(s) 78, 127, 227, 429, 434
MabI ACCWGGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 413, 571
MboII GAAGA 6 cut(s) 94, 211, 212, 373, 641, 644
MhlI GDGCHC 3 cut(s) 26, 213, 518
MluCI AATT 3 cut(s) 55, 203, 249
MlyI GAGTC 2 cut(s) 410, 531
MnlI CCTC 6 cut(s) 107, 119, 143, 180, 353, 535
MspR9I CCNGG 3 cut(s) 215, 319, 402
Mva1269I GAATGC 1 cut(s) 266
MvaI CCWGG 3 cut(s) 215, 319, 402
NcoI CCATGG 1 cut(s) 425
NlaIII CATG 5 cut(s) 78, 127, 227, 429, 434
NlaIV GGNNCC 2 cut(s) 589, 621
NmuCI GTSAC 1 cut(s) 413
NspI RCATGY 1 cut(s) 434
PctI GAATGC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 317
PleI GAGTC 2 cut(s) 410, 530
PpsI GAGTC 2 cut(s) 410, 530
PpuMI RGGWCCY 1 cut(s) 620
Psp124BI GAGCTC 2 cut(s) 26, 518
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 3 cut(s) 213, 317, 400
PspGI CCWGG 3 cut(s) 213, 317, 400
PspN4I GGNNCC 2 cut(s) 589, 621
PspPI GGNCC 2 cut(s) 588, 620
PspPPI RGGWCCY 1 cut(s) 620
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacI GAGCTC 2 cut(s) 26, 518
Sau96I GGNCC 2 cut(s) 588, 620
SchI GAGTC 2 cut(s) 410, 531
ScrFI CCNGG 3 cut(s) 215, 319, 402
SduI GDGCHC 3 cut(s) 26, 213, 518
SetI ASST 8 cut(s) 26, 111, 180, 216, 482, 501, 518, 644
SexAI ACCWGGT 1 cut(s) 213
SfcI CTRYAG 2 cut(s) 304, 624
SinI GGWCC 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 3 cut(s) 55, 203, 249
SsiI CCGC 1 cut(s) 68
SstI GAGCTC 2 cut(s) 26, 518
StyD4I CCNGG 3 cut(s) 213, 317, 400
StyI CCWWGG 3 cut(s) 145, 425, 480
TaaI ACNGT 1 cut(s) 308
TaqI TCGA 3 cut(s) 17, 59, 525
TaqII GACCGA 1 cut(s) 595
TasI AATT 3 cut(s) 55, 203, 249
TatI WGTACW 1 cut(s) 453
TscAI CASTG 2 cut(s) 362, 568
TseFI GTSAC 1 cut(s) 413
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 3 cut(s) 53, 63, 212
TspRI CASTG 2 cut(s) 362, 568
VneI GTGCAC 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 434
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.