Rorug01G0402500

Cytochrome b-561 / ferric reductase transmembrane domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0007411
Physical Location & Seq
Reverse (-)
50998989 .. 51004880
5892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0402500.1

Sequence Viewer

Length: 1731 bp
ATGGGGAAGAAAGCGAAGACGTCCAGGAAGGGAAAGAAGGCGTGGAGAGCCAACATTAGCACGGAGGACATCCAGGACTTCTTCGAGCAGTCCACCAAAGACGCTCTCTCCGGCGGCAATCTCTCTCAGGCTCCCGATGAAGCTCTCTTCTTCGTCGACAACTCCAAAGATCTTTCGGTGAAGAGGAAGATTGAAAAGCATAGAGAGAAAGTGCTTCACATTGACAGCATGCTACAGAAAAACCCATTTGTAAAAGCAGTGCCTTCTTCAACACTAAAGAAGTCCAAGAAAAAGCGAAAACAGATTGCAAATGCCAAAGATGTGACTGAATGCGGCACTGAGGGTTCTGTCTCAACTTCTGGCATGGCTGACCTATGGGGTGATAAAGGTGGTGACAATCGTGTGGTCAAGAAGATGATGAAGCCTTCACCTATTCCTGCTGTAGAAGTTGAGCCTCCAGGTTGCTCATATAATCCCACAGATGAAAGTCATCAGGAAGCATTGGCTCATGCTGTTGCAGATGAAATGCAGAAAGTCTATAGAAATGAGCTAGGGCCTGAACCAGTGCCTCTAACTGTTCCAGGGGAGACTATTGATGAAGAAGAAATGTATTTTATTGAAGCAGATGAAGGCAGTGATGATGATATGAATCCAGAAAACTTGGATGAGAATGAGCATGGTGCATCTGAGAAAAGGCCCTTTAAAACAAAGAGGGTGACAACAGTCGAATTAAATAAGAGAGCTAGGCGTAAAGAACAGCTGAAAAGGGAAGCAGAAGCAAAGAAGGTGCAGAAGCTTTCCAAAGAAATTGATGGCTTACCAGATATCCTTCAGGAAATAGCTGAAGACGACGAGGAGAAGCTAAAAAGACATCTTCGACGAGTTGTAGCTAAAAAGGAAAAATCTAAGTCATGTCCCCCGCGCTTGGGGAAGCACAAATTTGAACCTGCCCCAGTTCAAGTCCTTCTGACTGAAGAAAGAACTGGATCCATCCGTAAACTGAAGGGTTGTTGCACCCTTGTCAAGGATCGCTTCAAGAGTCTTGAAAAAAGAGGATTAATCCCAGTGAAGGCCAACAAAAGACCCTTCTTCAACCTCGGCGAGCATCGGAGTAATCTGTCGGGCACTATGCGCACGTTAGCAACCAAACTCACCACTTGTGCAAAGCATTACTGTAGAACAAACCCAGTACGGAATGCGCAGTCTCGCAGTTTCTCATCTTACAATGGAAGAGATGAGCTCTCACTTGAACAAGAAGCTGATAGAAAAATCGGGTGGTTGCTGAAGTTGTTATTTGCTGGCACTGCCACTGCTGTAGCTTACAACATCATGCCATATATGGGAGATAATTTGTTGCAACAGTCGGTGTCGCTTTTGCAAGTGAAGGATCCTTTGTTCAAGAGGATGGGGGCTTCTAGATTAGCGCAGTTTGCGATAGATGATGAAAGGAGGATGAAAATAGTGGAGATGGGTGGTGCTCAGGAGCTCGTAAATATGTTGGGGAATGCTAAAGATGACAGCACAAGGAAGGAAGCTTTGAAAGCTCTTTCTGCTATTTCACAATCAGATCAAGCTCTTGGAGCTTTACACCAAGCTGGGGCAATCTCAGTCATCCAAGCTGCCCCTGATTCCTTGGAACATGCCGACATTGAGAAATACAAGTCAGGTTTGCTCCAGAGATTCCAAGATCTGAGATATGATAGTCCTTCTGAAGATGTATCAAGTCCCTAG

Protein Analysis

576

Amino Acids

64.4

Weight (kDa)

9.05

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop53 PF07767 8 - 348 1.5e-60 Nop53 (60S ribosomal biogenesis)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 23
Acc16I TGCGCA 2 cut(s) 1133, 1200
Acc36I ACCTGC 1 cut(s) 955
AccI GTMKAC 1 cut(s) 156
AccII CGCG 1 cut(s) 922
AciI CCGC 3 cut(s) 114, 333, 920
AclWI GGATC 5 cut(s) 981, 994, 1035, 1382, 1395
AcsI RAATTY 1 cut(s) 938
AcuI CTGAAG 6 cut(s) 815, 864, 993, 1022, 1304, 1731
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 1 cut(s) 1191
AfiI CCNNNNNNNGG 6 cut(s) 925, 926, 1024, 1069, 1340, 1597
AjnI CCWGG 4 cut(s) 23, 72, 457, 580
AloI GAACNNNNNNTCC 2 cut(s) 1379, 1411
Alw21I GWGCWC 3 cut(s) 1242, 1480, 1488
Alw26I GTCTC 3 cut(s) 355, 581, 1209
AlwI GGATC 5 cut(s) 981, 994, 1035, 1382, 1395
AoxI GGCC 3 cut(s) 554, 695, 1071
ApeKI GCWGC 1 cut(s) 1619
ApoI RAATTY 1 cut(s) 938
AseI ATTAAT 1 cut(s) 1058
AspLEI GCGC 4 cut(s) 924, 1134, 1201, 1426
AspS9I GGNCC 2 cut(s) 554, 696
AsuHPI GGTGA 6 cut(s) 190, 392, 404, 420, 727, 1144
BaeGI GKGCMC 1 cut(s) 1127
BamHI GGATCC 2 cut(s) 986, 1387
BanII GRGCYC 2 cut(s) 1242, 1488
BbsI GAAGAC 2 cut(s) 23, 852
Bbv12I GWGCWC 3 cut(s) 1242, 1480, 1488
BbvI GCAGC 1 cut(s) 1606
BccI CCATC 4 cut(s) 806, 998, 1399, 1462
BciT130I CCWGG 4 cut(s) 25, 74, 459, 582
BcoDI GTCTC 3 cut(s) 355, 581, 1209
BfaI CTAG 4 cut(s) 551, 744, 1416, 1729
BfmI CTRYAG 5 cut(s) 233, 441, 538, 1174, 1314
BfuAI ACCTGC 1 cut(s) 955
BglII AGATCT 2 cut(s) 169, 1687
BisI GCNGC 3 cut(s) 115, 334, 1620
BlsI GCNGC 3 cut(s) 116, 335, 1621
Bme1390I CCNGG 4 cut(s) 25, 74, 459, 582
BmgT120I GGNCC 2 cut(s) 554, 696
BmiI GGNNCC 3 cut(s) 132, 988, 1389
BmrFI CCNGG 4 cut(s) 25, 74, 459, 582
BmrI ACTGGG 3 cut(s) 947, 1058, 1181
BmsI GCATC 2 cut(s) 692, 1114
BmuI ACTGGG 3 cut(s) 947, 1058, 1181
BoxI GACNNNNGTC 1 cut(s) 722
BpiI GAAGAC 2 cut(s) 23, 852
BplI GAGNNNNNCTC 2 cut(s) 1224, 1256
BpmI CTGGAG 2 cut(s) 441, 1658
Bpu10I CCTNAGC 1 cut(s) 1479
BsaBI GATNNNNATC 1 cut(s) 648
BsaHI GRCGYC 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 581, 1096, 1632
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 6 cut(s) 925, 926, 1024, 1069, 1340, 1597
Bse1I ACTGG 5 cut(s) 563, 953, 988, 1064, 1187
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 4 cut(s) 25, 74, 459, 582
BseDI CCNNGG 3 cut(s) 581, 1096, 1632
BseGI GGATG 6 cut(s) 69, 670, 990, 1410, 1458, 1611
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 6 cut(s) 925, 926, 1024, 1069, 1340, 1597
BseMII CTCAG 6 cut(s) 140, 330, 678, 1493, 1620, 1682
BseNI ACTGG 5 cut(s) 563, 953, 988, 1064, 1187
BseRI GAGGAG 1 cut(s) 869
BseSI GKGCMC 1 cut(s) 1127
BseXI GCAGC 1 cut(s) 1606
BseYI CCCAGC 1 cut(s) 1595
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 1 cut(s) 922
BshFI GGCC 3 cut(s) 556, 697, 1073
BsiHKAI GWGCWC 3 cut(s) 1242, 1480, 1488
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 2 cut(s) 900, 1710
BslI CCNNNNNNNGG 6 cut(s) 925, 926, 1024, 1069, 1340, 1597
BsmAI GTCTC 3 cut(s) 355, 581, 1209
BsmFI GGGAC 2 cut(s) 900, 1710
BsmI GAATGC 3 cut(s) 335, 1201, 1510
BsnI GGCC 3 cut(s) 556, 697, 1073
Bsp1286I GDGCHC 4 cut(s) 1127, 1242, 1480, 1488
Bsp143I GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
BspACI CCGC 3 cut(s) 114, 333, 920
BspANI GGCC 3 cut(s) 556, 697, 1073
BspCNI CTCAG 6 cut(s) 139, 331, 679, 1492, 1619, 1683
BspFNI CGCG 1 cut(s) 922
BspLI GGNNCC 3 cut(s) 132, 988, 1389
BspMI ACCTGC 1 cut(s) 955
BspPI GGATC 5 cut(s) 981, 994, 1035, 1382, 1395
BsrI ACTGG 5 cut(s) 563, 953, 988, 1064, 1187
BssECI CCNNGG 3 cut(s) 581, 1096, 1632
BssMI GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
BssNI GRCGYC 1 cut(s) 20
BssT1I CCWWGG 1 cut(s) 1632
Bst2UI CCWGG 4 cut(s) 25, 74, 459, 582
Bst4CI ACNGT 4 cut(s) 577, 724, 1175, 1362
Bst6I CTCTTC 3 cut(s) 152, 176, 1225
BstACI GRCGYC 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 230, 1103, 1300
BstDEI CTNAG 7 cut(s) 126, 339, 687, 906, 1479, 1606, 1691
BstENI CCTNNNNNAGG 1 cut(s) 1022
BstF5I GGATG 6 cut(s) 69, 670, 990, 1410, 1458, 1611
BstFNI CGCG 1 cut(s) 922
BstHHI GCGC 4 cut(s) 924, 1134, 1201, 1426
BstKTI GATC 6 cut(s) 172, 989, 1030, 1390, 1570, 1690
BstMAI GTCTC 3 cut(s) 355, 581, 1209
BstMBI GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
BstMWI GCNNNNNNNGC 7 cut(s) 47, 1131, 1304, 1430, 1541, 1550, 1580
BstNI CCWGG 4 cut(s) 25, 74, 459, 582
BstNSI RCATGY 2 cut(s) 232, 1643
BstPAI GACNNNNGTC 1 cut(s) 722
BstSCI CCNGG 4 cut(s) 23, 72, 457, 580
BstSFI CTRYAG 5 cut(s) 233, 441, 538, 1174, 1314
BstSLI GKGCMC 1 cut(s) 1127
BstUI CGCG 1 cut(s) 922
BstV1I GCAGC 1 cut(s) 1606
BstV2I GAAGAC 2 cut(s) 23, 852
BstX2I RGATCY 4 cut(s) 169, 986, 1387, 1687
BstYI RGATCY 4 cut(s) 169, 986, 1387, 1687
BsuRI GGCC 3 cut(s) 556, 697, 1073
BtsCI GGATG 6 cut(s) 69, 670, 990, 1410, 1458, 1611
BtsI GCAGTG 4 cut(s) 264, 640, 1302, 1308
BtsIMutI CAGTG 7 cut(s) 264, 336, 570, 640, 1071, 1302, 1308
BveI ACCTGC 1 cut(s) 955
Cac8I GCNNGC 3 cut(s) 230, 1103, 1300
CfoI GCGC 4 cut(s) 924, 1134, 1201, 1426
Cfr13I GGNCC 2 cut(s) 554, 696
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 1190
CviAII CATG 7 cut(s) 229, 364, 509, 677, 912, 1330, 1640
CviQI GTAC 1 cut(s) 1190
DdeI CTNAG 7 cut(s) 126, 339, 687, 906, 1479, 1606, 1691
DpnI GATC 6 cut(s) 171, 988, 1029, 1389, 1569, 1689
DpnII GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
DraI TTTAAA 1 cut(s) 703
Eam1104I CTCTTC 3 cut(s) 152, 176, 1225
EarI CTCTTC 3 cut(s) 152, 176, 1225
Ecl136II GAGCTC 2 cut(s) 1240, 1486
Eco130I CCWWGG 1 cut(s) 1632
Eco24I GRGCYC 2 cut(s) 1242, 1488
Eco32I GATATC 1 cut(s) 826
Eco53kI GAGCTC 2 cut(s) 1240, 1486
Eco57I CTGAAG 6 cut(s) 815, 864, 993, 1022, 1304, 1731
EcoICRI GAGCTC 2 cut(s) 1240, 1486
EcoNI CCTNNNNNAGG 1 cut(s) 1022
EcoO109I RGGNCCY 2 cut(s) 554, 696
EcoRII CCWGG 4 cut(s) 23, 72, 457, 580
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 1632
EcoT38I GRGCYC 2 cut(s) 1242, 1488
ErhI CCWWGG 1 cut(s) 1632
FaeI CATG 7 cut(s) 232, 367, 512, 680, 915, 1333, 1643
FalI AAGNNNNNCTT 2 cut(s) 1016, 1048
FaqI GGGAC 2 cut(s) 900, 1710
FatI CATG 7 cut(s) 228, 363, 508, 676, 911, 1329, 1639
FauI CCCGC 1 cut(s) 927
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 115, 334, 1620
FokI GGATG 6 cut(s) 56, 677, 977, 1417, 1465, 1598
FriOI GRGCYC 2 cut(s) 1242, 1488
Fsp4HI GCNGC 3 cut(s) 115, 334, 1620
FspAI RTGCGCAY 1 cut(s) 1133
FspBI CTAG 4 cut(s) 551, 744, 1416, 1729
FspI TGCGCA 2 cut(s) 1133, 1200
GlaI GCGC 4 cut(s) 923, 1133, 1200, 1425
GluI GCNGC 3 cut(s) 115, 334, 1620
GsaI CCCAGC 1 cut(s) 1599
GsuI CTGGAG 2 cut(s) 441, 1658
HaeIII GGCC 3 cut(s) 556, 697, 1073
HapII CCGG 1 cut(s) 111
HgaI GACGC 1 cut(s) 110
HhaI GCGC 4 cut(s) 924, 1134, 1201, 1426
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 7 cut(s) 232, 367, 512, 680, 915, 1333, 1643
Hin6I GCGC 4 cut(s) 922, 1132, 1199, 1424
HinP1I GCGC 4 cut(s) 922, 1132, 1199, 1424
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 2 cut(s) 794, 1533
HinfI GANTC 4 cut(s) 649, 1039, 1628, 1680
HpaII CCGG 1 cut(s) 111
HphI GGTGA 6 cut(s) 190, 392, 404, 420, 727, 1144
Hpy166II GTNNAC 3 cut(s) 93, 157, 998
Hpy188I TCNGA 6 cut(s) 688, 969, 1110, 1567, 1692, 1711
Hpy8I GTNNAC 3 cut(s) 93, 157, 998
Hpy99I CGWCG 3 cut(s) 158, 854, 882
HpyCH4III ACNGT 4 cut(s) 577, 724, 1175, 1362
HpyCH4IV ACGT 2 cut(s) 20, 1136
HpyCH4V TGCA 9 cut(s) 308, 518, 529, 683, 790, 1014, 1163, 1357, 1378
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 1131, 1304, 1430, 1541, 1550, 1580
HpyF3I CTNAG 7 cut(s) 126, 339, 687, 906, 1479, 1606, 1691
HpySE526I ACGT 2 cut(s) 20, 1136
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 7 cut(s) 232, 367, 512, 680, 915, 1333, 1643
HspAI GCGC 4 cut(s) 922, 1132, 1199, 1424
Kzo9I GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
LmnI GCTCC 4 cut(s) 136, 1483, 1580, 1677
Lsp1109I GCAGC 1 cut(s) 1606
LweI GCATC 2 cut(s) 692, 1114
MaeI CTAG 4 cut(s) 551, 744, 1416, 1729
MaeII ACGT 2 cut(s) 20, 1136
MaeIII GTNAC 3 cut(s) 322, 392, 715
MalI GATC 6 cut(s) 171, 988, 1029, 1389, 1569, 1689
MboI GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
MflI RGATCY 4 cut(s) 169, 986, 1387, 1687
MhlI GDGCHC 4 cut(s) 1127, 1242, 1480, 1488
MluCI AATT 4 cut(s) 728, 807, 938, 1348
MlyI GAGTC 1 cut(s) 1048
MseI TTAA 3 cut(s) 702, 731, 1058
MspA1I CMGCKG 1 cut(s) 760
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 4 cut(s) 25, 74, 459, 582
Mva1269I GAATGC 3 cut(s) 335, 1201, 1510
MvaI CCWGG 4 cut(s) 25, 74, 459, 582
MvnI CGCG 1 cut(s) 922
MwoI GCNNNNNNNGC 7 cut(s) 47, 1131, 1304, 1430, 1541, 1550, 1580
NdeII GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
NlaIII CATG 7 cut(s) 232, 367, 512, 680, 915, 1333, 1643
NlaIV GGNNCC 3 cut(s) 132, 988, 1389
NmeAIII GCCGAG 1 cut(s) 1077
NmuCI GTSAC 3 cut(s) 322, 392, 715
NsbI TGCGCA 2 cut(s) 1133, 1200
NspI RCATGY 2 cut(s) 232, 1643
PaeI GCATGC 1 cut(s) 232
PctI GAATGC 3 cut(s) 335, 1201, 1510
PfeI GAWTC 3 cut(s) 649, 1628, 1680
PfoI TCCNGGA 2 cut(s) 23, 72
PkrI GCNGC 3 cut(s) 116, 335, 1621
PleI GAGTC 1 cut(s) 1047
PpsI GAGTC 1 cut(s) 1047
PshAI GACNNNNGTC 1 cut(s) 722
PshBI ATTAAT 1 cut(s) 1058
Psp124BI GAGCTC 2 cut(s) 1242, 1488
Psp6I CCWGG 4 cut(s) 23, 72, 457, 580
PspFI CCCAGC 1 cut(s) 1595
PspGI CCWGG 4 cut(s) 23, 72, 457, 580
PspN4I GGNNCC 3 cut(s) 132, 988, 1389
PspPI GGNCC 2 cut(s) 554, 696
PsuI RGATCY 4 cut(s) 169, 986, 1387, 1687
PvuII CAGCTG 1 cut(s) 760
RsaI GTAC 1 cut(s) 1191
RsaNI GTAC 1 cut(s) 1190
SacI GAGCTC 2 cut(s) 1242, 1488
SalI GTCGAC 1 cut(s) 155
SaqAI TTAA 3 cut(s) 702, 731, 1058
SatI GCNGC 3 cut(s) 115, 334, 1620
Sau3AI GATC 6 cut(s) 169, 986, 1027, 1387, 1567, 1687
Sau96I GGNCC 2 cut(s) 554, 696
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 4 cut(s) 25, 74, 459, 582
SduI GDGCHC 4 cut(s) 1127, 1242, 1480, 1488
SfaNI GCATC 2 cut(s) 692, 1114
SfcI CTRYAG 5 cut(s) 233, 441, 538, 1174, 1314
SphI GCATGC 1 cut(s) 232
Sse9I AATT 4 cut(s) 728, 807, 938, 1348
SsiI CCGC 3 cut(s) 114, 333, 920
SspMI CTAG 4 cut(s) 551, 744, 1416, 1729
SstI GAGCTC 2 cut(s) 1242, 1488
StyD4I CCNGG 4 cut(s) 23, 72, 457, 580
StyI CCWWGG 1 cut(s) 1632
TaaI ACNGT 4 cut(s) 577, 724, 1175, 1362
TaiI ACGT 2 cut(s) 23, 1139
TaqI TCGA 4 cut(s) 84, 156, 726, 877
TasI AATT 4 cut(s) 728, 807, 938, 1348
TauI GCSGC 2 cut(s) 117, 336
TfiI GAWTC 3 cut(s) 649, 1628, 1680
Tru1I TTAA 3 cut(s) 702, 731, 1058
Tru9I TTAA 3 cut(s) 702, 731, 1058
TscAI CASTG 7 cut(s) 264, 343, 570, 640, 1071, 1309, 1315
TseFI GTSAC 3 cut(s) 322, 392, 715
TseI GCWGC 1 cut(s) 1619
Tsp45I GTSAC 3 cut(s) 322, 392, 715
TspDTI ATGAA 9 cut(s) 153, 434, 498, 537, 612, 642, 662, 1458, 1469
TspGWI ACGGA 3 cut(s) 77, 983, 1207
TspRI CASTG 7 cut(s) 264, 343, 570, 640, 1071, 1309, 1315
VspI ATTAAT 1 cut(s) 1058
XagI CCTNNNNNAGG 1 cut(s) 1022
XapI RAATTY 1 cut(s) 938
XbaI TCTAGA 1 cut(s) 1415
XceI RCATGY 2 cut(s) 232, 1643
XmiI GTMKAC 1 cut(s) 156
XspI CTAG 4 cut(s) 551, 744, 1416, 1729
ZraI GACGTC 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.