MD07G1268400.v1.1

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007411
Physical Location & Seq
Reverse (-)
33596978 .. 33600556
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1268400.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGCGCTAAAGGTACCGGCGCTTCCGTTGACGTTTGTGGTACACACGCTCGGAATCGTGGGCTTGGTTTTGGTGTTGGTTTGGAACATTCATTTCAGAGGCGGCTTGGCATGGGAAGCTGCCAACAAGAACCTCATCTTCAACCTCCATCCTGTTCTCATGTTCCTTGGCCTAATTTTCTTGGGAGGAGAAGCCATTGTTAGTTACAAAGCACTGCCTTTCAGGAAAGAGGTGAAGAAAGTGATACACTTGGTTCTTCATGCCCTCGCGTTAGGTCTTGGGATCATTGGTATTTATCTTGCATTCAAGAACCATAACGAGAGTGGAATTGCCAATCTGTACAGTTTGCACTCTTGGATTGGAATCGGGGTCATTTGTCTCTATGGAATTCAGTGGATATATGGGTTTGTGACCTTCTTCTACCCCGGAGGAAGTCCTGCACTGAGGAGCGAGTCTATCCCATGGCATGTGGTGTTTGGAATCACCGTGTACTTACTAGCTGTTGGCAATGCTTCATTAGGGTTCTTAGAGAAGGTCACATTCCTCGAGAACTCGGGACTCGATAAATTTGGGACGGAGGCCTTTCTCGTCAACTTCACCGCAATCATCACAGTACTGTTCGGAGTCTTCGTTATATTCTCAGTACTTTCTGAAGCTCCTACTGTAGAAGATGACAACAGCTATTCTGCTATTTGA

Protein Analysis

232

Amino Acids

25.27

Weight (kDa)

6.7

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 49 - 181 2.1e-47 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 13
AccB1I GGYRCC 1 cut(s) 13
AccII CGCG 1 cut(s) 269
AciI CCGC 2 cut(s) 102, 600
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 2 cut(s) 387, 566
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 6 cut(s) 15, 42, 341, 491, 615, 645
AgsI TTSAA 2 cut(s) 142, 307
AluBI AGCT 4 cut(s) 119, 500, 656, 681
AluI AGCT 4 cut(s) 119, 500, 656, 681
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 2 cut(s) 545, 553
AoxI GGCC 2 cut(s) 169, 579
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 2 cut(s) 387, 566
Asp718I GGTACC 1 cut(s) 13
AspLEI GCGC 2 cut(s) 7, 22
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 3 cut(s) 244, 475, 589
AvaI CYCGRG 2 cut(s) 545, 553
BanI GGYRCC 1 cut(s) 13
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 619
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 156
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 2 cut(s) 8, 23
BisI GCNGC 2 cut(s) 103, 120
BlsI GCNGC 2 cut(s) 104, 121
BmcAI AGTACT 2 cut(s) 615, 645
Bme1390I CCNGG 1 cut(s) 426
BmeT110I CYCGRG 2 cut(s) 545, 553
BmiI GGNNCC 1 cut(s) 15
BmrFI CCNGG 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 619
BpuMI CCSGG 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 362
BsaJI CCNNGG 3 cut(s) 166, 424, 461
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bse118I RCCGGY 1 cut(s) 16
Bse3DI GCAATG 1 cut(s) 514
Bse8I GATNNNNATC 1 cut(s) 362
BseDI CCNNGG 3 cut(s) 166, 424, 461
BseGI GGATG 1 cut(s) 148
BseJI GATNNNNATC 1 cut(s) 362
BseMI GCAATG 1 cut(s) 514
BseMII CTCAG 2 cut(s) 434, 654
BseRI GAGGAG 2 cut(s) 201, 460
BseXI GCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 2 cut(s) 171, 581
BshNI GGYRCC 1 cut(s) 13
BsiHKCI CYCGRG 2 cut(s) 545, 553
BsiSI CCGG 2 cut(s) 17, 426
BslFI GGGAC 2 cut(s) 570, 586
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 570, 586
BsmI GAATGC 1 cut(s) 302
BsnI GGCC 2 cut(s) 171, 581
BsoBI CYCGRG 2 cut(s) 545, 553
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 282
Bsp19I CCATGG 1 cut(s) 461
BspACI CCGC 2 cut(s) 102, 600
BspANI GGCC 2 cut(s) 171, 581
BspCNI CTCAG 2 cut(s) 435, 653
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 15
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 514
BsrFI RCCGGY 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 3 cut(s) 166, 424, 461
BssMI GATC 1 cut(s) 282
BssT1I CCWWGG 2 cut(s) 166, 461
Bst4CI ACNGT 5 cut(s) 344, 487, 613, 618, 664
BstAUI TGTACA 1 cut(s) 339
BstDEI CTNAG 3 cut(s) 443, 526, 640
BstDSI CCRYGG 1 cut(s) 461
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 2 cut(s) 8, 23
BstHHI GCGC 2 cut(s) 7, 22
BstKTI GATC 1 cut(s) 285
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 1 cut(s) 282
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 1 cut(s) 106
BstV2I GAAGAC 1 cut(s) 619
BsuRI GGCC 2 cut(s) 171, 581
BtgI CCRYGG 1 cut(s) 461
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 212
BtsIMutI CAGTG 3 cut(s) 212, 398, 440
CfoI GCGC 2 cut(s) 7, 22
Cfr10I RCCGGY 1 cut(s) 16
Csp6I GTAC 6 cut(s) 14, 41, 340, 490, 614, 644
CviAII CATG 5 cut(s) 111, 160, 260, 462, 467
CviJI RGCY 9 cut(s) 63, 105, 119, 171, 194, 500, 581, 656, 681
CviKI_1 RGCY 9 cut(s) 63, 105, 119, 171, 194, 500, 581, 656, 681
CviQI GTAC 6 cut(s) 14, 41, 340, 490, 614, 644
DdeI CTNAG 3 cut(s) 443, 526, 640
DpnI GATC 1 cut(s) 284
DpnII GATC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 166, 461
Eco147I AGGCCT 1 cut(s) 581
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 2 cut(s) 545, 553
EcoRI GAATTC 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 166, 461
ErhI CCWWGG 2 cut(s) 166, 461
FaeI CATG 5 cut(s) 114, 163, 263, 465, 470
FaqI GGGAC 2 cut(s) 570, 586
FatI CATG 5 cut(s) 110, 159, 259, 461, 466
Fnu4HI GCNGC 2 cut(s) 103, 120
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 103, 120
FspBI CTAG 1 cut(s) 497
GlaI GCGC 2 cut(s) 6, 21
GluI GCNGC 2 cut(s) 103, 120
HaeII RGCGCY 2 cut(s) 8, 23
HaeIII GGCC 2 cut(s) 171, 581
HapII CCGG 2 cut(s) 17, 426
HhaI GCGC 2 cut(s) 7, 22
Hin1II CATG 5 cut(s) 114, 163, 263, 465, 470
Hin6I GCGC 2 cut(s) 5, 20
HinP1I GCGC 2 cut(s) 5, 20
HincII GTYRAC 2 cut(s) 30, 592
HindII GTYRAC 2 cut(s) 30, 592
HinfI GANTC 6 cut(s) 54, 363, 452, 480, 558, 624
HpaII CCGG 2 cut(s) 17, 426
HphI GGTGA 3 cut(s) 244, 475, 589
Hpy166II GTNNAC 4 cut(s) 30, 43, 490, 592
Hpy188I TCNGA 4 cut(s) 53, 98, 623, 652
Hpy188III TCNNGA 4 cut(s) 223, 307, 547, 555
Hpy8I GTNNAC 4 cut(s) 30, 43, 490, 592
HpyAV CCTTC 2 cut(s) 424, 526
HpyCH4III ACNGT 5 cut(s) 344, 487, 613, 618, 664
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 302, 349, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 443, 526, 640
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 5 cut(s) 114, 163, 263, 465, 470
HspAI GCGC 2 cut(s) 5, 20
KpnI GGTACC 1 cut(s) 17
Kzo9I GATC 1 cut(s) 282
LmnI GCTCC 2 cut(s) 447, 661
LpnPI CCDG 5 cut(s) 30, 165, 208, 439, 450
Lsp1109I GCAGC 1 cut(s) 106
MaeI CTAG 1 cut(s) 497
MaeII ACGT 1 cut(s) 32
MaeIII GTNAC 3 cut(s) 203, 409, 535
MalI GATC 1 cut(s) 284
MboI GATC 1 cut(s) 282
MboII GAAGA 6 cut(s) 130, 247, 248, 409, 619, 680
MluCI AATT 4 cut(s) 174, 327, 387, 566
MlyI GAGTC 3 cut(s) 461, 552, 633
MspI CCGG 2 cut(s) 17, 426
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 302
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 426
NcoI CCATGG 1 cut(s) 461
NdeII GATC 1 cut(s) 282
NlaIII CATG 5 cut(s) 114, 163, 263, 465, 470
NlaIV GGNNCC 1 cut(s) 15
NmuCI GTSAC 2 cut(s) 409, 535
NspI RCATGY 1 cut(s) 470
PaeR7I CTCGAG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 581
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PctI GAATGC 1 cut(s) 302
PfeI GAWTC 3 cut(s) 54, 363, 480
PkrI GCNGC 2 cut(s) 104, 121
PleI GAGTC 3 cut(s) 460, 552, 632
PpsI GAGTC 3 cut(s) 460, 552, 632
PspN4I GGNNCC 1 cut(s) 15
RsaI GTAC 6 cut(s) 15, 42, 341, 491, 615, 645
RsaNI GTAC 6 cut(s) 14, 41, 340, 490, 614, 644
SatI GCNGC 2 cut(s) 103, 120
Sau3AI GATC 1 cut(s) 282
ScaI AGTACT 2 cut(s) 615, 645
SchI GAGTC 3 cut(s) 461, 552, 633
ScrFI CCNGG 1 cut(s) 426
SfcI CTRYAG 1 cut(s) 663
Sfr274I CTCGAG 1 cut(s) 545
SlaI CTCGAG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 4 cut(s) 174, 327, 387, 566
SseBI AGGCCT 1 cut(s) 581
SsiI CCGC 2 cut(s) 102, 600
SspMI CTAG 1 cut(s) 497
StuI AGGCCT 1 cut(s) 581
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 2 cut(s) 166, 461
TaaI ACNGT 5 cut(s) 344, 487, 613, 618, 664
TaiI ACGT 1 cut(s) 35
TaqI TCGA 2 cut(s) 546, 561
TasI AATT 4 cut(s) 174, 327, 387, 566
TatI WGTACW 4 cut(s) 339, 489, 613, 643
TauI GCSGC 1 cut(s) 105
TfiI GAWTC 3 cut(s) 54, 363, 480
TscAI CASTG 3 cut(s) 219, 398, 447
TseFI GTSAC 2 cut(s) 409, 535
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 2 cut(s) 409, 535
TspDTI ATGAA 3 cut(s) 80, 248, 504
TspGWI ACGGA 2 cut(s) 15, 590
TspRI CASTG 3 cut(s) 219, 398, 447
XapI RAATTY 2 cut(s) 387, 566
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 320
XhoI CTCGAG 1 cut(s) 545
XspI CTAG 1 cut(s) 497
ZrmI AGTACT 2 cut(s) 615, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.