Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001511

Physical Location & Seq

Genomic Coordinates Reverse (-)

30065016 .. 30071325

Gene Structure 6310 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1205500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2706 bp

ATGAGAAATATGCCTTCATGGTGGGGGAAGTCGTCATCCAAAGAAGCAAAGAAGAAGGCAGGAAAGGAAAGTTTTATTGACTCTTTACATCGGAAATTTAATTTTTCTTCAGAGAGTAGATTGAATAGTAGATCAGGAGGGTCTCGAAGAAATTGCAATGATACTATTTCGGAAAAAGGATGTCAATCCCCTATAGAATCAATATCTCCTTCGCCCTCAAAAAATGTTTCCAGATGTCAAAGTTTTGCTGAAAGGACTAATGCTCAACCACTTCCGCTTCCTGGTCTACATGTTGCTCATGTTGCTCGTACAGATTCTGGAATTAGTATATCAACAAAACCAAGATCGGAAAAAGGCTCTAAGCCATTATCGTTTCTACCTCTGCCGAGACCTGCATGCATCGGCAGTAGGTCAAATCGTACAGATGTAGATGGAGACATGGTCACTGATTCAGTCTTTAGTGAGAGCTCTGTTGATAGTGACGATCCAGCTGACTCATCATGCCATCGTAGCCCTCAGGCAACTGATGACAATGGCACTAGAACAGCTTCTGGCAGCCCTTGCAGTGTGATGCTCAAGGATCAGTCATTCAATGTTGCTCCAGTACACTCAAGAGAGCCAAAAAAATCAGCTAACATTTCTTTCAATAATCATGTTTCCCCCACATCGCCTAAGCGTAGGCATCTAGGCAGCCATGTGCCAAACCTACAAGTTCCTTATAATGGAGCTGTCTGCAGTGCTCCTGACAGCTCAATGTCAAGTCCTTCTAGAAGCCCAATGAGAGCATTTGGCACTGAGCAAGTGGTGAACTCTGCTTTCTGGGCTGCAAAGCCTTATCCAGATGTCACTCTACTTGGCTCTGGCCACTGCTCCAGTCCAGGTTCGGGTCACAATTCTGGACATAATTCAATGGGAGGAGATATGTCTGGACAGTTGTTTTGGCAGCAAAGCAGGGGTAGCCCTGAGTATTCTCCGGTACCTAGTCCCAGAATGACAAGCCCAGGCCCAGGCTCAAGAATTCATAGTGGTGCTGTTACGCCTATCCATCCTAAAGCAGGAGGGACTCCCAATGAGACACAGACAAGCTGGGCTGATGATGGGAAACAACAAAGTCACCGTTTGCCTCTTCCCCCTGTGAATGCTTCCCCTTTTTCTCATTCAAATTCAGCAGCAACATCTCCTTCTGTGCCACGAAGCCCAGGAAGGGCAGAGAACCCACCAAGCCCTGGTTCACGCTGGAAAAAGGGAAAGCTGCTGGGTAGAGGGACTTTTGGACATGTTTATGTTGGTTTTAATAGTGAAAGTGGTGAAATGTGTGCGATGAAGGAGGTCACATTATTTTCTGATGATGCAAAGTCTAAGGAAAGCGCTAAGCAATTAAATCAGGAAATTGCTTTATTGAGCCGTTTACGGCACCCCAATATTGTGCAATATTATGGGTCTGAATCAGTTGATGACAAGCTATATATATATTTGGAGTATGTATCTGGTGGATCCATATATAAACTTCTCCAAGACTATGGGCAATTTGGCGAACTAGCAATTCGTAGTTATACTAAGCAAATCTTGTCGGGGCTGGCGTATTTACATGCTAAAAACACTGTCCACAGGGATATTAAAGGGGCAAATATACTTGTAGACCCAAATGGACGTGTCAAGTTGGCAGACTTTGGGATGGCTAAGCATATCACTGGGCAGTCATGTCCATTATCATTTAAGGGAAGCCCTTACTGGATGGCACCTGAGGTTATAAAGAACTCAAGTGGTGCCAACCTTGCTGTAGATATATGGAGTCTTGGGTGCACTGTTTTGGAAATGGCTACAACAAAACCACCTTGGAGCCAGTATGAAGGGGTTGCTGCCATGTTTAAGATTGGGAATAGCAAGGAACTTCCAGCAATTCCAGATAGCCTCTTGGATGATGGAAAGGATTTCATAAGGCAATGCTTACAGAGAAATCCACTCCATCGTCCTACAGCTGCCCAGCTTTTGGAGCATCCTTATGTGAAATATGCTGCGCCTTTGGAAAGATCTATGTTGGGCCTTGAGCCTTCTGATCCACCCTCTGGGATTACAAATGGAGTGAAAACACTGGGTATTGGGCAAGGGAGGAATTTCTCCAACTTGGATTCAGATAGGCTTGCAATTCATTCATCTAGACATTCAAAAACTAATAACCATACCAGTGAGATCCATATTCCAAGGAATATATCATGCCCTGTCTCACCCATTGGAAGCCCTCTATTGCACTCAAGGTCACCACCACACCTAAATGGAAGAATGTCACCTTCCCCTATATCTAGCCCACGGACTACTTCGGGCTCATCCACGCCTCTCACAGGTGGCAGTGGTGCCGTTCCATTTATTCACATGAACCAGCCCATCAACTTGCAAGAGGGTTTTGGAGGCATTTCAAAACCCTCGAATGGTCTCTATGTCAATGGGCCCTCCTCCTATCATGATTCATGTCCTGACATGTTTCGAGGGAAGCAGCCAGGCGCTCATATCTTCCCAGAACTGATGCCTTGTGAAAATGATGTTCTGGGAAAGCAGTTTGTAAGGCCTGCCAAAGTGGAGCAGTATGATGGACAGTCGGTTTTAGCTGATCGGGTGTCTCGCCAGCTCCTTAAGGATCATGTTAAAATGAACCTGAAACTGGATTTAAGCCCCAACTCTCCTTTGCCGAACCGCACAAGTGGTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

902

Amino Acids

97.21

Weight (kDa)

9.32

Isoelectric Point (pI)

63.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	414 - 669	2e-71	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	415 - 665	4.7e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63700 AT1G63700
fragaria_vesca	FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920
malus_domestica	MD01G1205500.v1.1 MD06G1085900.v1.1 MD07G1274900.v1.1
prunus_persica	Prupe.2G298100_v2.0.a1 Prupe.2G298100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1
pyrus_communis	pycom01g21500 pycom06g08220 pycom07g25100
rosa_chinensis	RchiOBHm_Chr1g0377881 RchiOBHm_Chr7g0197281
rosa_laevigata	RLG00000003944 RLG00000026489
rosa_multiflora	Rmu_co8459889.1_g000001 Rmu_sc0004491.1_g000001 Rmu_sc0004824.1_g000011 Rmu_sc0010019.1_g000003 Rmu_sc0017623.1_g000004 Rmu_sc0030897.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258510 Rroxscaffold_4G00280980
rosa_rugosa	Rorug01G0405400 Rorug07G0039200
rosa_samantha	Rh1AG426400 Rh1BG382900 Rh1CG396700 Rh1DG413900 Rh7AG164200 Rh7BG166000 Rh7CG172700 Rh7DG165400
rosa_wichuraiana	Rw1G037210 Rw7G014320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	720, 1751
Acc36I	ACCTGC	1 cut(s)	400
Acc65I	GGTACC	1 cut(s)	976
AccB1I	GGYRCC	5 cut(s)	976, 1413, 1738, 1766, 2351
AccB7I	CCANNNNNTGG	3 cut(s)	1226, 1990, 2066
AccI	GTMKAC	2 cut(s)	286, 1638
AciI	CCGC	2 cut(s)	275, 2689
AclWI	GGATC	7 cut(s)	479, 588, 1488, 1501, 2051, 2185, 2640
AcoI	YGGCCR	1 cut(s)	862
AcsI	RAATTY	4 cut(s)	95, 1017, 1162, 2113
AcuI	CTGAAG	1 cut(s)	93
AfaI	GTAC	4 cut(s)	310, 421, 606, 978
AfeI	AGCGCT	1 cut(s)	1369
AflII	CTTAAG	1 cut(s)	2627
AflIII	ACRYGT	4 cut(s)	289, 1276, 1651, 2475
AgsI	TTSAA	7 cut(s)	124, 592, 646, 909, 1161, 2166, 2415
AjiI	CACGTC	1 cut(s)	1652
AjnI	CCWGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
AjuI	GAANNNNNNNTTGG	2 cut(s)	1213, 1245
Alw21I	GWGCWC	3 cut(s)	470, 742, 1805
Alw26I	GTCTC	7 cut(s)	147, 382, 429, 1067, 2227, 2435, 2619
Alw44I	GTGCAC	1 cut(s)	1801
AlwI	GGATC	7 cut(s)	479, 588, 1488, 1501, 2051, 2185, 2640
AlwNI	CAGNNNCTG	2 cut(s)	317, 551
Aor51HI	AGCGCT	1 cut(s)	1369
AoxI	GGCC	5 cut(s)	862, 1003, 2041, 2444, 2561
ApaI	GGGCCC	1 cut(s)	2448
ApaLI	GTGCAC	1 cut(s)	1801
ApoI	RAATTY	4 cut(s)	95, 1017, 1162, 2113
ArsI	GACNNNNNNTTYG	2 cut(s)	921, 953
Asp700I	GAANNNNTTC	1 cut(s)	547
Asp718I	GGTACC	1 cut(s)	976
AspLEI	GCGC	3 cut(s)	1370, 2020, 2501
AspS9I	GGNCC	4 cut(s)	1004, 2041, 2444, 2445
AsuHPI	GGTGA	6 cut(s)	817, 1106, 1319, 2217, 2250, 2277
AxyI	CCTNAGG	2 cut(s)	516, 1743
BaeGI	GKGCMC	2 cut(s)	1805, 2448
BalI	TGGCCA	1 cut(s)	864
BamHI	GGATCC	1 cut(s)	1493
BanI	GGYRCC	5 cut(s)	976, 1413, 1738, 1766, 2351
BanII	GRGCYC	3 cut(s)	470, 2324, 2448
Bbv12I	GWGCWC	3 cut(s)	470, 742, 1805
BceAI	ACGGC	3 cut(s)	1389, 1427, 2339
BcgI	CGANNNNNNTGC	2 cut(s)	135, 169
BciT130I	CCWGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
BcoDI	GTCTC	7 cut(s)	147, 382, 429, 1067, 2227, 2435, 2619
BfaI	CTAG	7 cut(s)	540, 686, 768, 981, 1538, 2157, 2301
BfmI	CTRYAG	4 cut(s)	192, 733, 1779, 1974
BfoI	RGCGCY	2 cut(s)	1371, 2502
BfrI	CTTAAG	1 cut(s)	2627
BfuAI	ACCTGC	1 cut(s)	400
BglII	AGATCT	1 cut(s)	2030
BlpI	GCTNAGC	2 cut(s)	1371, 1680
Bme1390I	CCNGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
BmgBI	CACGTC	1 cut(s)	1652
BmgT120I	GGNCC	4 cut(s)	1004, 2041, 2444, 2445
BmiI	GGNNCC	8 cut(s)	978, 1415, 1495, 1740, 1768, 1841, 2353, 2446
BmrFI	CCNGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
BmrI	ACTGGG	2 cut(s)	1701, 2102
BmsI	GCATC	6 cut(s)	408, 561, 691, 1339, 2005, 2511
BmuI	ACTGGG	2 cut(s)	1701, 2102
BpmI	CTGGAG	2 cut(s)	585, 856
Bpu10I	CCTNAGC	1 cut(s)	672
Bpu1102I	GCTNAGC	2 cut(s)	1371, 1680
BpuEI	CTTGAG	6 cut(s)	560, 595, 997, 1744, 2066, 2236
BsaBI	GATNNNNATC	1 cut(s)	184
BsaI	GGTCTC	3 cut(s)	147, 382, 2435
BsaJI	CCNNGG	7 cut(s)	1000, 1006, 1198, 1225, 1835, 2201, 2306
BsaWI	WCCGGW	1 cut(s)	973
BsaXI	ACNNNNNCTCC	2 cut(s)	426, 456
Bse1I	ACTGG	8 cut(s)	602, 873, 1696, 1736, 1843, 2097, 2184, 2661
Bse21I	CCTNAGG	2 cut(s)	516, 1743
Bse3DI	GCAATG	2 cut(s)	163, 1949
Bse8I	GATNNNNATC	1 cut(s)	184
BseBI	CCWGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
BseDI	CCNNGG	7 cut(s)	1000, 1006, 1198, 1225, 1835, 2201, 2306
BseGI	GGATG	8 cut(s)	35, 185, 1045, 1680, 1740, 1924, 1996, 2324
BseJI	GATNNNNATC	1 cut(s)	184
BseMI	GCAATG	2 cut(s)	163, 1949
BseMII	CTCAG	4 cut(s)	530, 786, 954, 1734
BseNI	ACTGG	8 cut(s)	602, 873, 1696, 1736, 1843, 2097, 2184, 2661
BseRI	GAGGAG	2 cut(s)	930, 2440
BseSI	GKGCMC	2 cut(s)	1805, 2448
BseYI	CCCAGC	3 cut(s)	1086, 1255, 1983
BshFI	GGCC	5 cut(s)	864, 1005, 2043, 2446, 2563
BshNI	GGYRCC	5 cut(s)	976, 1413, 1738, 1766, 2351
BsiHKAI	GWGCWC	3 cut(s)	470, 742, 1805
BsiSI	CCGG	1 cut(s)	974
BslFI	GGGAC	3 cut(s)	969, 1075, 1279
BsmAI	GTCTC	7 cut(s)	147, 382, 429, 1067, 2227, 2435, 2619
BsmFI	GGGAC	3 cut(s)	969, 1075, 1279
BsmI	GAATGC	1 cut(s)	1144
BsnI	GGCC	5 cut(s)	864, 1005, 2043, 2446, 2563
Bso31I	GGTCTC	3 cut(s)	147, 382, 2435
Bsp120I	GGGCCC	1 cut(s)	2444
Bsp1286I	GDGCHC	5 cut(s)	470, 742, 1805, 2324, 2448
Bsp1720I	GCTNAGC	2 cut(s)	1371, 1680
BspACI	CCGC	2 cut(s)	275, 2689
BspANI	GGCC	5 cut(s)	864, 1005, 2043, 2446, 2563
BspCNI	CTCAG	4 cut(s)	529, 787, 955, 1735
BspHI	TCATGA	1 cut(s)	2458
BspLI	GGNNCC	8 cut(s)	978, 1415, 1495, 1740, 1768, 1841, 2353, 2446
BspMAI	CTGCAG	1 cut(s)	737
BspMI	ACCTGC	1 cut(s)	400
BspPI	GGATC	7 cut(s)	479, 588, 1488, 1501, 2051, 2185, 2640
BspT107I	GGYRCC	5 cut(s)	976, 1413, 1738, 1766, 2351
BspTI	CTTAAG	1 cut(s)	2627
BspTNI	GGTCTC	3 cut(s)	147, 382, 2435
BsrDI	GCAATG	2 cut(s)	163, 1949
BsrI	ACTGG	8 cut(s)	602, 873, 1696, 1736, 1843, 2097, 2184, 2661
BssECI	CCNNGG	7 cut(s)	1000, 1006, 1198, 1225, 1835, 2201, 2306
BssT1I	CCWWGG	2 cut(s)	1835, 2201
Bst2UI	CCWGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
Bst4CI	ACNGT	5 cut(s)	933, 1118, 1603, 1807, 2592
Bst6I	CTCTTC	1 cut(s)	1131
BstAFI	CTTAAG	1 cut(s)	2627
BstAPI	GCANNNNNTGC	1 cut(s)	561
BstC8I	GCNNGC	5 cut(s)	397, 1578, 2142, 2565, 2621
BstDSI	CCRYGG	1 cut(s)	2306
BstEII	GGTNACC	1 cut(s)	2256
BstENI	CCTNNNNNAGG	2 cut(s)	1053, 2337
BstF5I	GGATG	8 cut(s)	35, 185, 1045, 1680, 1740, 1924, 1996, 2324
BstH2I	RGCGCY	2 cut(s)	1371, 2502
BstHHI	GCGC	3 cut(s)	1370, 2020, 2501
BstMAI	GTCTC	7 cut(s)	147, 382, 429, 1067, 2227, 2435, 2619
BstMWI	GCNNNNNNNGC	7 cut(s)	302, 510, 561, 821, 957, 1775, 1993
BstNI	CCWGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
BstNSI	RCATGY	5 cut(s)	293, 399, 1280, 1592, 2479
BstPI	GGTNACC	1 cut(s)	2256
BstSCI	CCNGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
BstSFI	CTRYAG	4 cut(s)	192, 733, 1779, 1974
BstSLI	GKGCMC	2 cut(s)	1805, 2448
BstX2I	RGATCY	3 cut(s)	1493, 2030, 2190
BstXI	CCANNNNNNTGG	1 cut(s)	1520
BstYI	RGATCY	3 cut(s)	1493, 2030, 2190
Bsu36I	CCTNAGG	2 cut(s)	516, 1743
BsuRI	GGCC	5 cut(s)	864, 1005, 2043, 2446, 2563
BtgI	CCRYGG	1 cut(s)	2306
BtgZI	GCGATG	2 cut(s)	651, 1334
BtrI	CACGTC	1 cut(s)	1652
BtsCI	GGATG	8 cut(s)	35, 185, 1045, 1680, 1740, 1924, 1996, 2324
BtsI	GCAGTG	4 cut(s)	571, 742, 865, 2353
BveI	ACCTGC	1 cut(s)	400
Cac8I	GCNNGC	5 cut(s)	397, 1578, 2142, 2565, 2621
CaiI	CAGNNNCTG	2 cut(s)	317, 551
CciI	TCATGA	1 cut(s)	2458
CfoI	GCGC	3 cut(s)	1370, 2020, 2501
Cfr13I	GGNCC	4 cut(s)	1004, 2041, 2444, 2445
Csp6I	GTAC	4 cut(s)	309, 420, 605, 977
CviQI	GTAC	4 cut(s)	309, 420, 605, 977
EaeI	YGGCCR	1 cut(s)	862
Eam1104I	CTCTTC	1 cut(s)	1131
EarI	CTCTTC	1 cut(s)	1131
Ecl136II	GAGCTC	1 cut(s)	468
Eco130I	CCWWGG	2 cut(s)	1835, 2201
Eco147I	AGGCCT	1 cut(s)	2563
Eco24I	GRGCYC	3 cut(s)	470, 2324, 2448
Eco31I	GGTCTC	3 cut(s)	147, 382, 2435
Eco47III	AGCGCT	1 cut(s)	1369
Eco53kI	GAGCTC	1 cut(s)	468
Eco57I	CTGAAG	1 cut(s)	93
Eco81I	CCTNAGG	2 cut(s)	516, 1743
Eco91I	GGTNACC	1 cut(s)	2256
EcoICRI	GAGCTC	1 cut(s)	468
EcoNI	CCTNNNNNAGG	2 cut(s)	1053, 2337
EcoO109I	RGGNCCY	1 cut(s)	2445
EcoO65I	GGTNACC	1 cut(s)	2256
EcoRI	GAATTC	1 cut(s)	1017
EcoRII	CCWGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
EcoT14I	CCWWGG	2 cut(s)	1835, 2201
EcoT22I	ATGCAT	1 cut(s)	401
EcoT38I	GRGCYC	3 cut(s)	470, 2324, 2448
ErhI	CCWWGG	2 cut(s)	1835, 2201
FalI	AAGNNNNNCTT	2 cut(s)	1550, 1582
FaqI	GGGAC	3 cut(s)	969, 1075, 1279
FblI	GTMKAC	2 cut(s)	286, 1638
FokI	GGATG	8 cut(s)	22, 192, 1032, 1687, 1747, 1931, 1983, 2311
FriOI	GRGCYC	3 cut(s)	470, 2324, 2448
FspBI	CTAG	7 cut(s)	540, 686, 768, 981, 1538, 2157, 2301
GlaI	GCGC	3 cut(s)	1369, 2019, 2500
GsaI	CCCAGC	3 cut(s)	1090, 1259, 1987
GsuI	CTGGAG	2 cut(s)	585, 856
HaeII	RGCGCY	2 cut(s)	1371, 2502
HaeIII	GGCC	5 cut(s)	864, 1005, 2043, 2446, 2563
HapII	CCGG	1 cut(s)	974
HhaI	GCGC	3 cut(s)	1370, 2020, 2501
Hin6I	GCGC	3 cut(s)	1368, 2018, 2499
HinP1I	GCGC	3 cut(s)	1368, 2018, 2499
HpaII	CCGG	1 cut(s)	974
HphI	GGTGA	6 cut(s)	817, 1106, 1319, 2217, 2250, 2277
Hpy166II	GTNNAC	8 cut(s)	287, 607, 808, 1232, 1409, 1606, 1639, 1803
Hpy188I	TCNGA	8 cut(s)	93, 112, 172, 349, 1345, 1444, 2056, 2134
Hpy8I	GTNNAC	8 cut(s)	287, 607, 808, 1232, 1409, 1606, 1639, 1803
HpyCH4III	ACNGT	5 cut(s)	933, 1118, 1603, 1807, 2592
HpyCH4IV	ACGT	1 cut(s)	1651
HpyF10VI	GCNNNNNNNGC	7 cut(s)	302, 510, 561, 821, 957, 1775, 1993
HpySE526I	ACGT	1 cut(s)	1651
HspAI	GCGC	3 cut(s)	1368, 2018, 2499
KpnI	GGTACC	1 cut(s)	980
LmnI	GCTCC	8 cut(s)	604, 725, 745, 875, 1839, 1993, 2575, 2628
LweI	GCATC	6 cut(s)	408, 561, 691, 1339, 2005, 2511
MaeI	CTAG	7 cut(s)	540, 686, 768, 981, 1538, 2157, 2301
MaeII	ACGT	1 cut(s)	1651
MaeIII	GTNAC	9 cut(s)	442, 479, 844, 887, 1033, 1112, 1330, 2256, 2283
MboII	GAAGA	6 cut(s)	64, 99, 159, 1118, 2289, 2500
MflI	RGATCY	3 cut(s)	1493, 2030, 2190
MhlI	GDGCHC	5 cut(s)	470, 742, 1805, 2324, 2448
MlsI	TGGCCA	1 cut(s)	864
MluNI	TGGCCA	1 cut(s)	864
MlyI	GAGTC	4 cut(s)	74, 488, 1057, 1801
MmeI	TCCRAC	1 cut(s)	2145
Mox20I	TGGCCA	1 cut(s)	864
Mph1103I	ATGCAT	1 cut(s)	401
MroXI	GAANNNNTTC	1 cut(s)	547
MscI	TGGCCA	1 cut(s)	864
MseI	TTAA	9 cut(s)	99, 1293, 1379, 1617, 1716, 1869, 2628, 2640, 2663
MslI	CAYNNNNRTG	5 cut(s)	1026, 1281, 2001, 2184, 2271
Msp20I	TGGCCA	1 cut(s)	864
MspA1I	CMGCKG	2 cut(s)	491, 1979
MspCI	CTTAAG	1 cut(s)	2627
MspI	CCGG	1 cut(s)	974
MspR9I	CCNGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
Mva1269I	GAATGC	1 cut(s)	1144
MvaI	CCWGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
MwoI	GCNNNNNNNGC	7 cut(s)	302, 510, 561, 821, 957, 1775, 1993
NlaIV	GGNNCC	8 cut(s)	978, 1415, 1495, 1740, 1768, 1841, 2353, 2446
NmeAIII	GCCGAG	1 cut(s)	411
NmuCI	GTSAC	8 cut(s)	442, 479, 844, 887, 1112, 1330, 2256, 2283
NsiI	ATGCAT	1 cut(s)	401
NspI	RCATGY	5 cut(s)	293, 399, 1280, 1592, 2479
PaeI	GCATGC	1 cut(s)	399
PagI	TCATGA	1 cut(s)	2458
PceI	AGGCCT	1 cut(s)	2563
PciI	ACATGT	3 cut(s)	289, 1276, 2475
PcsI	WCGNNNNNNNCGW	1 cut(s)	1577
PctI	GAATGC	1 cut(s)	1144
PdmI	GAANNNNTTC	1 cut(s)	547
PfeI	GAWTC	6 cut(s)	197, 314, 449, 1445, 2129, 2462
PflFI	GACNNNGTC	1 cut(s)	440
PflMI	CCANNNNNTGG	3 cut(s)	1226, 1990, 2066
PleI	GAGTC	4 cut(s)	74, 488, 1057, 1800
PpsI	GAGTC	4 cut(s)	74, 488, 1057, 1800
PscI	ACATGT	3 cut(s)	289, 1276, 2475
PsiI	TTATAA	2 cut(s)	720, 1751
Psp124BI	GAGCTC	1 cut(s)	470
Psp6I	CCWGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
PspEI	GGTNACC	1 cut(s)	2256
PspFI	CCCAGC	3 cut(s)	1086, 1255, 1983
PspGI	CCWGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
PspN4I	GGNNCC	8 cut(s)	978, 1415, 1495, 1740, 1768, 1841, 2353, 2446
PspOMI	GGGCCC	1 cut(s)	2444
PspPI	GGNCC	4 cut(s)	1004, 2041, 2444, 2445
PstI	CTGCAG	1 cut(s)	737
PstNI	CAGNNNCTG	2 cut(s)	317, 551
PsuI	RGATCY	3 cut(s)	1493, 2030, 2190
PsyI	GACNNNGTC	1 cut(s)	440
PvuII	CAGCTG	2 cut(s)	491, 1979
RsaI	GTAC	4 cut(s)	310, 421, 606, 978
RsaNI	GTAC	4 cut(s)	309, 420, 605, 977
RseI	CAYNNNNRTG	5 cut(s)	1026, 1281, 2001, 2184, 2271
SacI	GAGCTC	1 cut(s)	470
SaqAI	TTAA	9 cut(s)	99, 1293, 1379, 1617, 1716, 1869, 2628, 2640, 2663
Sau96I	GGNCC	4 cut(s)	1004, 2041, 2444, 2445
SchI	GAGTC	4 cut(s)	74, 488, 1057, 1801
ScrFI	CCNGG	7 cut(s)	282, 879, 1002, 1008, 1200, 1227, 2496
SduI	GDGCHC	5 cut(s)	470, 742, 1805, 2324, 2448
SfaNI	GCATC	6 cut(s)	408, 561, 691, 1339, 2005, 2511
SfcI	CTRYAG	4 cut(s)	192, 733, 1779, 1974
SmiMI	CAYNNNNRTG	5 cut(s)	1026, 1281, 2001, 2184, 2271
SmlI	CTYRAG	7 cut(s)	575, 610, 1012, 1759, 2045, 2251, 2627
SmoI	CTYRAG	7 cut(s)	575, 610, 1012, 1759, 2045, 2251, 2627
SphI	GCATGC	1 cut(s)	399
SseBI	AGGCCT	1 cut(s)	2563
SsiI	CCGC	2 cut(s)	275, 2689
SspI	AATATT	2 cut(s)	1423, 1433
SspMI	CTAG	7 cut(s)	540, 686, 768, 981, 1538, 2157, 2301
SstI	GAGCTC	1 cut(s)	470
StuI	AGGCCT	1 cut(s)	2563
StyD4I	CCNGG	7 cut(s)	280, 877, 1000, 1006, 1198, 1225, 2494
StyI	CCWWGG	2 cut(s)	1835, 2201
TaaI	ACNGT	5 cut(s)	933, 1118, 1603, 1807, 2592
TaiI	ACGT	1 cut(s)	1654
TaqI	TCGA	3 cut(s)	145, 2423, 2482
TatI	WGTACW	1 cut(s)	604
TfiI	GAWTC	6 cut(s)	197, 314, 449, 1445, 2129, 2462
Tru1I	TTAA	9 cut(s)	99, 1293, 1379, 1617, 1716, 1869, 2628, 2640, 2663
Tru9I	TTAA	9 cut(s)	99, 1293, 1379, 1617, 1716, 1869, 2628, 2640, 2663
TseFI	GTSAC	8 cut(s)	442, 479, 844, 887, 1112, 1330, 2256, 2283
Tsp45I	GTSAC	8 cut(s)	442, 479, 844, 887, 1112, 1330, 2256, 2283
TspGWI	ACGGA	1 cut(s)	2323
Tth111I	GACNNNGTC	1 cut(s)	440
Van91I	CCANNNNNTGG	3 cut(s)	1226, 1990, 2066
Vha464I	CTTAAG	1 cut(s)	2627
VneI	GTGCAC	1 cut(s)	1801
XagI	CCTNNNNNAGG	2 cut(s)	1053, 2337
XapI	RAATTY	4 cut(s)	95, 1017, 1162, 2113
XbaI	TCTAGA	2 cut(s)	767, 2156
XceI	RCATGY	5 cut(s)	293, 399, 1280, 1592, 2479
XmiI	GTMKAC	2 cut(s)	286, 1638
XmnI	GAANNNNTTC	1 cut(s)	547
XspI	CTAG	7 cut(s)	540, 686, 768, 981, 1538, 2157, 2301
Zsp2I	ATGCAT	1 cut(s)	401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.