Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001511

Physical Location & Seq

Genomic Coordinates Reverse (-)

21179882 .. 21186581

Gene Structure 6700 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1085900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGCCTTCTTGGTGGAGGAAGTCTTCATCCAAAGAAGTGAAGAAGAAGAAAGCTAATCGGGAGAGTTTCATCGATACCATAGCAACCATACACCGTAAACTTAAGAGTTCGTCCGAAGAAAAGTGCAATGGTAGATCAGGAGGTTCTCGAAGAAGATGCATTGACACCGTTTCAGAAATGGGTTCTCTATCTAGGGTGCTGTCACCAGCACCCTCCTCACAAGTATCACGCTGTCAGAGCTTTGCAGAAAAGCCTCATGCCCAGCCACTGCCCCTTCCTAGAGTGCAACTTTCTAACATTGGGTGTACTAACTCTGGCATCAGTGTGGCATCAAAACCAGAATCTGACAGAGAGTCCAACCAATTGTTCTATTTGCCTCTTCCAGCACCCGGATGTGTCTCAAGCAGGGAAGATCGTACCTATGCTGAAGGAGATTTAGCTACTGCTTCCATATCCAGTGATAGTTCTATTGATAGTGATGATCCGTCTGACTCACGCCTTCTAAGTCCCATGGGATCCGACTATGAAAATGGAAACAGAACAAATATAAACAGTCCTTCCTGTGTAATACAAAAGGATCCATTCCCTATTGTGTACCAAAAGAACTCCAAAGAAACGTTGAAGCCGGGTCATCTTTTGTGCAATAATCAGATTCTGTCTACATCACCAAAATGGAAACCTTCAAGCACACATATGCAGAATATACAAATCCCTTTCCATGGTACTTTGTCTAGTGCTCCAGACAGCTCAATGTCGAGTCCTTCTAGGAGTCCAATTAGAGTGTTCAGTGTTGAGCAAGTTAGGAACTCTAGTTTCTGGGCAGCAAAGACTTATCCAGATATAGCTTCTACCCACTGCTCTAGTCCAGGTTCAGGCCAAAATTCTGGGCATAATTCAGTTGGAGGGGATATGTCAGGACAGTTGTTTTGGCAGCACAGTAGGTGTAGTCCTGAATGTTCTCCAATACCCAGTCCTAGAATGACTAGTCCTGGTCCCAACTCCAGAATACAAAGTGGTGCTGCTACCCCTCTGCATCCTCGATCCGGAGGGCCAGCCGCAGAGTCACCTACAAGCCGGCCTGATGATGCGAAGCAGAAAAGCCATCGGTTGCCCCTTCCTCCAATAACAATAACTAAGACCTGTCTTTTTTCTCCTTCATATTCAGCTACAACAACTCCCACAGTTCCACGTAGCCCTAATAGGGTAGAACATCCAACAAGCCCTGGCTCACACTGGAAGAAGGGGCGCCTGCTTGGAAGGGGCACATTTGGACATGTATATCTTGGTTTTAACAGTGAAAGCGGTGAGATGTGTGCAATGAAGGAGGTAACTCTATTTGCAGATGATGCAAAATCAAAGGAAAGTGCACAGCAGCTGGGCCAAGAAATTGCTTTGCTAAGTCGCTTGCGACATCCAAATATAGTGCAGTATTATGGATCTGAGACGGTAGATGACAAGCTTTACATATACTTGGAGTATGTGTCTGGTGGGTCCATCTATAAACTGCTTCAAGAGTATGGTCAGTTTGGTGAAGTAGCCACTCGTAGTTATACCCAACAAATCTTGTCAGGGCTTGCATACTTACATACCAAAAACACTGTCCACCGGGATATCAAGGGAGCAAATATATTAGTAGATCCAAATGGTCGGGTGAAATTAGCAGATTTTGGGATGGCAAAGCATATAACTGGGCAGTCTTGTCCATTATCGTTCAATGGAAGCCCATATTGGATGGCGCCTGAGGTTATCAAGAATTCAAACGGTTGTAATCTTGCGGTTGATATATGGAGCCTTGGCTGCACTGTTCTTGAGATGGCCACAACAAAACCACCTTGGAGCCAATATGAAGGGGTTGCTGCCATGTTTAAGATTGGAAACAGCAAGGAACTTCCTGAAATTCCTGATCATCTGTCAGAGGATGGGAAGAACTTTATTAGGCTTTGTTTGCAACGTAACCCACTGCATCGTCCCACAGCTGCTCAGCTTTTGGAGCATCCTTTTGTTAGGAATGTTGCTCCACCGGAAAGAACCATTTCTAGTGCAGAGCATTCTAAAGGACAAGCTGCAATGAGATCTCAGGCATTTGGACATGGGAAAAATCATTTGAACTTAGAATCAGAACGGATGGGCATGCATCAGTCCAGAGGCTCAACAACTGTTTCTGCGTCAAGTGATGCTTATTCACCGAAAAATGTATCATGTCCAATTTCTCCTATTGGAAGTCCGCTTCTACATTCTAGATCACCACAACATGTCAGCGGAAGGATGTCCCCCTCTCCCATATCTAGCCCTCGTACCACATCCGGTTCATCTACGCCTCTCACCAGCTGTTCAATCCCTTTTCAACACTTGAAGCAGCCAACAACCTACTCACTTGCAGGCACAGGGATGACCCAGAGATCTCAAAACTGTGGTTTCCATACAAATGGCAACATGCCATACCATGAGCCAAAGCCAGACCTATCTCAAGGATTTCCACAAGGCTCTTATGCTTTCCGGGATATATTTTTATCTGATAATGGTGTACTTGGAAACCAGGTCGGGCATTCAGTTTCTGGGGACCCCAAGGAGCTATTTGACGTGCAGTCTGTTTTGGCTGATCGTGTGTCTCAGCAGCTCTTAAGGGATCATATTAAGTTGAACATCCATGGACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0009653 GO:0009791 GO:0009888 GO:0009893 GO:0009987 GO:0010016 GO:0010098 GO:0010103 GO:0010229 GO:0010374 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048367 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0090558 GO:0090567 GO:0090626 GO:0090698 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

886

Amino Acids

96.39

Weight (kDa)

9.1

Isoelectric Point (pI)

57.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	413 - 668	1.9e-71	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	415 - 664	1.9e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63700 AT1G63700
fragaria_vesca	FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920
malus_domestica	MD01G1205500.v1.1 MD06G1085900.v1.1 MD07G1274900.v1.1
prunus_persica	Prupe.2G298100_v2.0.a1 Prupe.2G298100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1
pyrus_communis	pycom01g21500 pycom06g08220 pycom07g25100
rosa_chinensis	RchiOBHm_Chr1g0377881 RchiOBHm_Chr7g0197281
rosa_laevigata	RLG00000003944 RLG00000026489
rosa_multiflora	Rmu_co8459889.1_g000001 Rmu_sc0004491.1_g000001 Rmu_sc0004824.1_g000011 Rmu_sc0010019.1_g000003 Rmu_sc0017623.1_g000004 Rmu_sc0030897.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258510 Rroxscaffold_4G00280980
rosa_rugosa	Rorug01G0405400 Rorug07G0039200
rosa_samantha	Rh1AG426400 Rh1BG382900 Rh1CG396700 Rh1DG413900 Rh7AG164200 Rh7BG166000 Rh7CG172700 Rh7DG165400
rosa_wichuraiana	Rw1G037210 Rw7G014320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1245, 1735
AccI	GTMKAC	1 cut(s)	659
AccIII	TCCGGA	1 cut(s)	1043
AciI	CCGC	5 cut(s)	1056, 1302, 1775, 2225, 2259
AclI	AACGTT	1 cut(s)	617
AclWI	GGATC	9 cut(s)	476, 510, 523, 572, 585, 1035, 1444, 1631, 2634
AcoI	YGGCCR	1 cut(s)	1815
AcsI	RAATTY	3 cut(s)	880, 1753, 1896
AcuI	CTGAAG	1 cut(s)	447
AcyI	GRCGYC	2 cut(s)	1246, 1736
AfaI	GTAC	6 cut(s)	307, 418, 596, 724, 2296, 2526
AfiI	CCNNNNNNNGG	5 cut(s)	389, 719, 872, 1043, 1201
AflII	CTTAAG	2 cut(s)	101, 2620
AflIII	ACRYGT	2 cut(s)	1273, 2251
AhdI	GACNNNNNGTC	2 cut(s)	352, 2584
AhlI	ACTAGT	1 cut(s)	983
AjiI	CACGTC	1 cut(s)	2581
AjnI	CCWGG	4 cut(s)	865, 988, 1222, 2535
AjuI	GAANNNNNNNTTGG	4 cut(s)	350, 382, 1711, 1743
Alw21I	GWGCWC	2 cut(s)	739, 1369
Alw26I	GTCTC	3 cut(s)	403, 1436, 2613
Alw44I	GTGCAC	1 cut(s)	1365
AlwI	GGATC	9 cut(s)	476, 510, 523, 572, 585, 1035, 1444, 1631, 2634
AlwNI	CAGNNNCTG	5 cut(s)	268, 344, 655, 1375, 2555
Aor13HI	TCCGGA	1 cut(s)	1043
AoxI	GGCC	5 cut(s)	874, 1049, 1076, 1378, 1815
ApaLI	GTGCAC	1 cut(s)	1365
ApoI	RAATTY	3 cut(s)	880, 1753, 1896
ArsI	GACNNNNNNTTYG	4 cut(s)	909, 941, 1584, 1616
Asp700I	GAANNNNTTC	1 cut(s)	2032
AspLEI	GCGC	2 cut(s)	1248, 1738
AspS9I	GGNCC	5 cut(s)	992, 1049, 1378, 1491, 2560
AsuC2I	CCSGG	4 cut(s)	390, 627, 1607, 2498
AsuHPI	GGTGA	9 cut(s)	195, 657, 1056, 1316, 1541, 1663, 2175, 2235, 2314
AvaII	GGWCC	3 cut(s)	992, 1491, 2560
AxyI	CCTNAGG	1 cut(s)	1740
BaeGI	GKGCMC	2 cut(s)	1265, 1369
BalI	TGGCCA	1 cut(s)	1817
BamHI	GGATCC	2 cut(s)	515, 577
BanI	GGYRCC	2 cut(s)	1245, 1735
BbsI	GAAGAC	1 cut(s)	15
Bbv12I	GWGCWC	2 cut(s)	739, 1369
BccI	CCATC	7 cut(s)	1110, 1502, 1666, 1726, 1807, 1913, 2119
BcgI	CGANNNNNNTGC	2 cut(s)	138, 172
BciT130I	CCWGG	4 cut(s)	867, 990, 1224, 2537
BclI	TGATCA	1 cut(s)	1903
BcnI	CCSGG	4 cut(s)	390, 627, 1607, 2498
BcoDI	GTCTC	3 cut(s)	403, 1436, 2613
BcuI	ACTAGT	1 cut(s)	983
BfmI	CTRYAG	1 cut(s)	2654
BfoI	RGCGCY	2 cut(s)	1249, 1739
BfrI	CTTAAG	2 cut(s)	101, 2620
BglII	AGATCT	2 cut(s)	2072, 2399
BlpI	GCTNAGC	1 cut(s)	1980
Bme1390I	CCNGG	8 cut(s)	390, 627, 867, 990, 1224, 1607, 2498, 2537
Bme18I	GGWCC	3 cut(s)	992, 1491, 2560
BmeRI	GACNNNNNGTC	2 cut(s)	352, 2584
BmgBI	CACGTC	1 cut(s)	2581
BmgT120I	GGNCC	5 cut(s)	992, 1049, 1378, 1491, 2560
BmrFI	CCNGG	8 cut(s)	390, 627, 867, 990, 1224, 1607, 2498, 2537
BmrI	ACTGGG	2 cut(s)	963, 1698
BmuI	ACTGGG	2 cut(s)	963, 1698
BpiI	GAAGAC	1 cut(s)	15
BpmI	CTGGAG	2 cut(s)	723, 985
Bpu1102I	GCTNAGC	1 cut(s)	1980
BpuEI	CTTGAG	3 cut(s)	385, 1829, 2451
BpuMI	CCSGG	4 cut(s)	390, 627, 1607, 2498
Bsa29I	ATCGAT	1 cut(s)	72
BsaAI	YACGTR	1 cut(s)	1190
BsaHI	GRCGYC	2 cut(s)	1246, 1736
BsaJI	CCNNGG	7 cut(s)	510, 718, 1222, 1792, 1832, 2565, 2647
BsaWI	WCCGGW	3 cut(s)	1043, 2020, 2303
BsaXI	ACNNNNNCTCC	4 cut(s)	1159, 1189, 1466, 1496
Bsc4I	CCNNNNNNNGG	5 cut(s)	389, 719, 872, 1043, 1201
Bse118I	RCCGGY	1 cut(s)	1074
Bse1I	ACTGG	4 cut(s)	456, 969, 1238, 1693
Bse21I	CCTNAGG	1 cut(s)	1740
Bse3DI	GCAATG	3 cut(s)	133, 1323, 2073
BseAI	TCCGGA	1 cut(s)	1043
BseBI	CCWGG	4 cut(s)	867, 990, 1224, 2537
BseCI	ATCGAT	1 cut(s)	72
BseDI	CCNNGG	7 cut(s)	510, 718, 1222, 1792, 1832, 2565, 2647
BseLI	CCNNNNNNNGG	5 cut(s)	389, 719, 872, 1043, 1201
BseMI	GCAATG	3 cut(s)	133, 1323, 2073
BseMII	CTCAG	5 cut(s)	1431, 1731, 1994, 2090, 2624
BseNI	ACTGG	4 cut(s)	456, 969, 1238, 1693
BseRI	GAGGAG	1 cut(s)	205
BseSI	GKGCMC	2 cut(s)	1265, 1369
BseYI	CCCAGC	2 cut(s)	261, 1375
BsgI	GTGCAG	4 cut(s)	1445, 1783, 2061, 2603
BshFI	GGCC	5 cut(s)	876, 1051, 1078, 1380, 1817
BshNI	GGYRCC	2 cut(s)	1245, 1735
BshVI	ATCGAT	1 cut(s)	72
BsiHKAI	GWGCWC	2 cut(s)	739, 1369
BsiSI	CCGG	8 cut(s)	390, 626, 1044, 1075, 1606, 2021, 2304, 2497
BslFI	GGGAC	5 cut(s)	492, 978, 1953, 2254, 2573
BslI	CCNNNNNNNGG	5 cut(s)	389, 719, 872, 1043, 1201
BsmAI	GTCTC	3 cut(s)	403, 1436, 2613
BsmBI	CGTCTC	1 cut(s)	1436
BsmFI	GGGAC	5 cut(s)	492, 978, 1953, 2254, 2573
BsmI	GAATGC	2 cut(s)	2047, 2545
BsnI	GGCC	5 cut(s)	876, 1051, 1078, 1380, 1817
Bsp1286I	GDGCHC	3 cut(s)	739, 1265, 1369
Bsp13I	TCCGGA	1 cut(s)	1043
Bsp1720I	GCTNAGC	1 cut(s)	1980
Bsp19I	CCATGG	3 cut(s)	510, 718, 2647
BspACI	CCGC	5 cut(s)	1056, 1302, 1775, 2225, 2259
BspANI	GGCC	5 cut(s)	876, 1051, 1078, 1380, 1817
BspCNI	CTCAG	5 cut(s)	1432, 1732, 1993, 2089, 2623
BspDI	ATCGAT	1 cut(s)	72
BspEI	TCCGGA	1 cut(s)	1043
BspPI	GGATC	9 cut(s)	476, 510, 523, 572, 585, 1035, 1444, 1631, 2634
BspT107I	GGYRCC	2 cut(s)	1245, 1735
BspTI	CTTAAG	2 cut(s)	101, 2620
BsrDI	GCAATG	3 cut(s)	133, 1323, 2073
BsrFI	RCCGGY	1 cut(s)	1074
BsrI	ACTGG	4 cut(s)	456, 969, 1238, 1693
BssAI	RCCGGY	1 cut(s)	1074
BssECI	CCNNGG	7 cut(s)	510, 718, 1222, 1792, 1832, 2565, 2647
BssNI	GRCGYC	2 cut(s)	1246, 1736
BssT1I	CCWWGG	6 cut(s)	510, 718, 1792, 1832, 2565, 2647
Bst2UI	CCWGG	4 cut(s)	867, 990, 1224, 2537
Bst6I	CTCTTC	1 cut(s)	384
BstACI	GRCGYC	2 cut(s)	1246, 1736
BstAFI	CTTAAG	2 cut(s)	101, 2620
BstAPI	GCANNNNNTGC	1 cut(s)	1346
BstBAI	YACGTR	1 cut(s)	1190
BstC8I	GCNNGC	7 cut(s)	1053, 1076, 1250, 1406, 1575, 2132, 2380
BstDEI	CTNAG	9 cut(s)	503, 1134, 1397, 1440, 1740, 1980, 2076, 2110, 2610
BstDSI	CCRYGG	3 cut(s)	510, 718, 2647
BstH2I	RGCGCY	2 cut(s)	1249, 1739
BstHHI	GCGC	2 cut(s)	1248, 1738
BstMAI	GTCTC	3 cut(s)	403, 1436, 2613
BstMWI	GCNNNNNNNGC	5 cut(s)	237, 1346, 1797, 1945, 1990
BstNI	CCWGG	4 cut(s)	867, 990, 1224, 2537
BstNSI	RCATGY	4 cut(s)	1277, 2134, 2255, 2437
BstSCI	CCNGG	8 cut(s)	388, 625, 865, 988, 1222, 1605, 2496, 2535
BstSFI	CTRYAG	1 cut(s)	2654
BstSLI	GKGCMC	2 cut(s)	1265, 1369
BstV2I	GAAGAC	1 cut(s)	15
BstX2I	RGATCY	6 cut(s)	515, 577, 1436, 1636, 2072, 2399
BstXI	CCANNNNNNTGG	2 cut(s)	884, 2426
BstYI	RGATCY	6 cut(s)	515, 577, 1436, 1636, 2072, 2399
Bsu15I	ATCGAT	1 cut(s)	72
Bsu36I	CCTNAGG	1 cut(s)	1740
BsuRI	GGCC	5 cut(s)	876, 1051, 1078, 1380, 1817
BsuTUI	ATCGAT	1 cut(s)	72
BtgI	CCRYGG	3 cut(s)	510, 718, 2647
BtrI	CACGTC	1 cut(s)	2581
BtsI	GCAGTG	3 cut(s)	266, 853, 1958
Cac8I	GCNNGC	7 cut(s)	1053, 1076, 1250, 1406, 1575, 2132, 2380
CaiI	CAGNNNCTG	5 cut(s)	268, 344, 655, 1375, 2555
CfoI	GCGC	2 cut(s)	1248, 1738
Cfr10I	RCCGGY	1 cut(s)	1074
Cfr13I	GGNCC	5 cut(s)	992, 1049, 1378, 1491, 2560
ClaI	ATCGAT	1 cut(s)	72
CseI	GACGC	1 cut(s)	2154
CsiI	ACCWGGT	1 cut(s)	2535
Csp6I	GTAC	6 cut(s)	306, 417, 595, 723, 2295, 2525
CviQI	GTAC	6 cut(s)	306, 417, 595, 723, 2295, 2525
DdeI	CTNAG	9 cut(s)	503, 1134, 1397, 1440, 1740, 1980, 2076, 2110, 2610
DinI	GGCGCC	2 cut(s)	1247, 1737
DriI	GACNNNNNGTC	2 cut(s)	352, 2584
EaeI	YGGCCR	1 cut(s)	1815
Eam1104I	CTCTTC	1 cut(s)	384
Eam1105I	GACNNNNNGTC	2 cut(s)	352, 2584
EarI	CTCTTC	1 cut(s)	384
Eco130I	CCWWGG	6 cut(s)	510, 718, 1792, 1832, 2565, 2647
Eco32I	GATATC	1 cut(s)	1612
Eco47I	GGWCC	3 cut(s)	992, 1491, 2560
Eco57I	CTGAAG	1 cut(s)	447
Eco81I	CCTNAGG	1 cut(s)	1740
EcoO109I	RGGNCCY	1 cut(s)	2560
EcoRI	GAATTC	1 cut(s)	1753
EcoRII	CCWGG	4 cut(s)	865, 988, 1222, 2535
EcoRV	GATATC	1 cut(s)	1612
EcoT14I	CCWWGG	6 cut(s)	510, 718, 1792, 1832, 2565, 2647
EcoT22I	ATGCAT	2 cut(s)	161, 2136
EgeI	GGCGCC	2 cut(s)	1247, 1737
EheI	GGCGCC	2 cut(s)	1247, 1737
ErhI	CCWWGG	6 cut(s)	510, 718, 1792, 1832, 2565, 2647
Esp3I	CGTCTC	1 cut(s)	1436
FalI	AAGNNNNNCTT	2 cut(s)	2161, 2193
FaqI	GGGAC	5 cut(s)	492, 978, 1953, 2254, 2573
FauNDI	CATATG	1 cut(s)	693
FbaI	TGATCA	1 cut(s)	1903
FblI	GTMKAC	1 cut(s)	659
GlaI	GCGC	2 cut(s)	1247, 1737
GsaI	CCCAGC	2 cut(s)	265, 1379
GsuI	CTGGAG	2 cut(s)	723, 985
HaeII	RGCGCY	2 cut(s)	1249, 1739
HaeIII	GGCC	5 cut(s)	876, 1051, 1078, 1380, 1817
HapII	CCGG	8 cut(s)	390, 626, 1044, 1075, 1606, 2021, 2304, 2497
HgaI	GACGC	1 cut(s)	2154
HhaI	GCGC	2 cut(s)	1248, 1738
Hin1I	GRCGYC	2 cut(s)	1246, 1736
Hin6I	GCGC	2 cut(s)	1246, 1736
HinP1I	GCGC	2 cut(s)	1246, 1736
HindIII	AAGCTT	1 cut(s)	1457
HinfI	GANTC	8 cut(s)	341, 353, 491, 652, 757, 769, 1061, 2114
HpaII	CCGG	8 cut(s)	390, 626, 1044, 1075, 1606, 2021, 2304, 2497
HphI	GGTGA	9 cut(s)	195, 657, 1056, 1316, 1541, 1663, 2175, 2235, 2314
Hpy166II	GTNNAC	7 cut(s)	98, 306, 595, 660, 1367, 1603, 2525
Hpy8I	GTNNAC	7 cut(s)	98, 306, 595, 660, 1367, 1603, 2525
HpyCH4IV	ACGT	4 cut(s)	617, 1189, 1951, 2580
HpyF10VI	GCNNNNNNNGC	5 cut(s)	237, 1346, 1797, 1945, 1990
HpyF3I	CTNAG	9 cut(s)	503, 1134, 1397, 1440, 1740, 1980, 2076, 2110, 2610
HpySE526I	ACGT	4 cut(s)	617, 1189, 1951, 2580
Hsp92I	GRCGYC	2 cut(s)	1246, 1736
HspAI	GCGC	2 cut(s)	1246, 1736
KasI	GGCGCC	2 cut(s)	1245, 1735
KflI	GGGWCCC	1 cut(s)	2560
Kpn2I	TCCGGA	1 cut(s)	1043
KroI	GCCGGC	1 cut(s)	1074
KroNI	GCCGGC	1 cut(s)	1076
Ksp22I	TGATCA	1 cut(s)	1903
LmnI	GCTCC	7 cut(s)	742, 1619, 1788, 1836, 1990, 2020, 2569
MabI	ACCWGGT	1 cut(s)	2535
MaeII	ACGT	4 cut(s)	617, 1189, 1951, 2580
MaeIII	GTNAC	4 cut(s)	201, 1062, 1327, 1952
MfeI	CAATTG	1 cut(s)	362
MflI	RGATCY	6 cut(s)	515, 577, 1436, 1636, 2072, 2399
MhlI	GDGCHC	3 cut(s)	739, 1265, 1369
MlsI	TGGCCA	1 cut(s)	1817
MluCI	AATT	9 cut(s)	362, 774, 880, 892, 1386, 1655, 1753, 1896, 2205
MluNI	TGGCCA	1 cut(s)	1817
Mly113I	GGCGCC	2 cut(s)	1246, 1736
MlyI	GAGTC	5 cut(s)	362, 485, 766, 778, 1070
MmeI	TCCRAC	4 cut(s)	381, 543, 880, 1238
Mox20I	TGGCCA	1 cut(s)	1817
Mph1103I	ATGCAT	2 cut(s)	161, 2136
MroI	TCCGGA	1 cut(s)	1043
MroNI	GCCGGC	1 cut(s)	1074
MroXI	GAANNNNTTC	1 cut(s)	2032
MscI	TGGCCA	1 cut(s)	1817
MseI	TTAA	5 cut(s)	102, 1290, 1866, 2621, 2634
MslI	CAYNNNNRTG	6 cut(s)	323, 391, 670, 692, 2387, 2424
Msp20I	TGGCCA	1 cut(s)	1817
MspA1I	CMGCKG	4 cut(s)	1375, 1976, 2259, 2328
MspCI	CTTAAG	2 cut(s)	101, 2620
MspI	CCGG	8 cut(s)	390, 626, 1044, 1075, 1606, 2021, 2304, 2497
MspR9I	CCNGG	8 cut(s)	390, 627, 867, 990, 1224, 1607, 2498, 2537
MunI	CAATTG	1 cut(s)	362
Mva1269I	GAATGC	2 cut(s)	2047, 2545
MvaI	CCWGG	4 cut(s)	867, 990, 1224, 2537
MwoI	GCNNNNNNNGC	5 cut(s)	237, 1346, 1797, 1945, 1990
NaeI	GCCGGC	1 cut(s)	1076
NarI	GGCGCC	2 cut(s)	1246, 1736
NciI	CCSGG	4 cut(s)	390, 627, 1607, 2498
NcoI	CCATGG	3 cut(s)	510, 718, 2647
NdeI	CATATG	1 cut(s)	693
NgoMIV	GCCGGC	1 cut(s)	1074
NmuCI	GTSAC	2 cut(s)	201, 1062
NsiI	ATGCAT	2 cut(s)	161, 2136
NspI	RCATGY	4 cut(s)	1277, 2134, 2255, 2437
PaeI	GCATGC	1 cut(s)	2134
PciI	ACATGT	2 cut(s)	1273, 2251
PctI	GAATGC	2 cut(s)	2047, 2545
PdiI	GCCGGC	1 cut(s)	1076
PdmI	GAANNNNTTC	1 cut(s)	2032
PfeI	GAWTC	3 cut(s)	341, 652, 2114
PfoI	TCCNGGA	1 cut(s)	2496
PleI	GAGTC	5 cut(s)	361, 485, 765, 777, 1069
PluTI	GGCGCC	2 cut(s)	1249, 1739
PpsI	GAGTC	5 cut(s)	361, 485, 765, 777, 1069
Ppu21I	YACGTR	1 cut(s)	1190
PpuMI	RGGWCCY	1 cut(s)	2560
PscI	ACATGT	2 cut(s)	1273, 2251
Psp1406I	AACGTT	1 cut(s)	617
Psp5II	RGGWCCY	1 cut(s)	2560
Psp6I	CCWGG	4 cut(s)	865, 988, 1222, 2535
PspFI	CCCAGC	2 cut(s)	261, 1375
PspGI	CCWGG	4 cut(s)	865, 988, 1222, 2535
PspPI	GGNCC	5 cut(s)	992, 1049, 1378, 1491, 2560
PspPPI	RGGWCCY	1 cut(s)	2560
PstNI	CAGNNNCTG	5 cut(s)	268, 344, 655, 1375, 2555
PsuI	RGATCY	6 cut(s)	515, 577, 1436, 1636, 2072, 2399
PvuII	CAGCTG	3 cut(s)	1375, 1976, 2328
RsaI	GTAC	6 cut(s)	307, 418, 596, 724, 2296, 2526
RsaNI	GTAC	6 cut(s)	306, 417, 595, 723, 2295, 2525
RseI	CAYNNNNRTG	6 cut(s)	323, 391, 670, 692, 2387, 2424
SaqAI	TTAA	5 cut(s)	102, 1290, 1866, 2621, 2634
Sau96I	GGNCC	5 cut(s)	992, 1049, 1378, 1491, 2560
SchI	GAGTC	5 cut(s)	362, 485, 766, 778, 1070
ScrFI	CCNGG	8 cut(s)	390, 627, 867, 990, 1224, 1607, 2498, 2537
SduI	GDGCHC	3 cut(s)	739, 1265, 1369
SexAI	ACCWGGT	1 cut(s)	2535
SfcI	CTRYAG	1 cut(s)	2654
SfoI	GGCGCC	2 cut(s)	1247, 1737
SinI	GGWCC	3 cut(s)	992, 1491, 2560
SmiMI	CAYNNNNRTG	6 cut(s)	323, 391, 670, 692, 2387, 2424
SmlI	CTYRAG	5 cut(s)	101, 400, 1808, 2466, 2620
SmoI	CTYRAG	5 cut(s)	101, 400, 1808, 2466, 2620
SpeI	ACTAGT	1 cut(s)	983
SphI	GCATGC	1 cut(s)	2134
Sse9I	AATT	9 cut(s)	362, 774, 880, 892, 1386, 1655, 1753, 1896, 2205
SsiI	CCGC	5 cut(s)	1056, 1302, 1775, 2225, 2259
SspDI	GGCGCC	2 cut(s)	1245, 1735
StyD4I	CCNGG	8 cut(s)	388, 625, 865, 988, 1222, 1605, 2496, 2535
StyI	CCWWGG	6 cut(s)	510, 718, 1792, 1832, 2565, 2647
TaiI	ACGT	4 cut(s)	620, 1192, 1954, 2583
TaqI	TCGA	4 cut(s)	72, 148, 755, 1039
TasI	AATT	9 cut(s)	362, 774, 880, 892, 1386, 1655, 1753, 1896, 2205
TatI	WGTACW	2 cut(s)	305, 2524
TauI	GCSGC	1 cut(s)	1058
TfiI	GAWTC	3 cut(s)	341, 652, 2114
Tru1I	TTAA	5 cut(s)	102, 1290, 1866, 2621, 2634
Tru9I	TTAA	5 cut(s)	102, 1290, 1866, 2621, 2634
TseFI	GTSAC	2 cut(s)	201, 1062
Tsp45I	GTSAC	2 cut(s)	201, 1062
TspDTI	ATGAA	7 cut(s)	15, 58, 540, 1146, 1334, 1860, 2298
TspGWI	ACGGA	2 cut(s)	474, 2137
Vha464I	CTTAAG	2 cut(s)	101, 2620
VneI	GTGCAC	1 cut(s)	1365
VpaK11BI	GGWCC	3 cut(s)	992, 1491, 2560
XapI	RAATTY	3 cut(s)	880, 1753, 1896
XbaI	TCTAGA	1 cut(s)	2237
XceI	RCATGY	4 cut(s)	1277, 2134, 2255, 2437
XmiI	GTMKAC	1 cut(s)	659
XmnI	GAANNNNTTC	1 cut(s)	2032
Zsp2I	ATGCAT	2 cut(s)	161, 2136

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.