Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001511

Physical Location & Seq

Genomic Coordinates Reverse (-)

34013176 .. 34019501

Gene Structure 6326 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1274900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGAGAAATATGCCTTCATGGTGGGGGAAGTCGTCGTCCAAAGAAGCAAAGAAGAAGGCAGGAAAGGAAAGTTTCATTGACTCTTTACATCGGAAATTTAAGTTTTCTTCAGAAAGTAGAGTGAATAATAGATCAGGAGGATCTCGAAGAAATTGCAATGACACAATTTCGGAAAAAGGATGTCAATCCCCTATAGAATCAAGATCTCCTTCACCCTCAAAAAATGTTGCCAGATGTCAAAGTTTTGCTGAAAGGACTAATGCTCAGCCACTTCCACTTCCTGGTCTACATCCTGCTCATGTTGCTCGTACAAATTCTGGAATTAGTATATCAACAAAACCGAGATCAGAAAAAGGCTCTAAGCCATTATCGTTTCTACCTCTCCCGAGACCTGCGTGCATCGGTAGTAGGTCAAATCATACAGACGTAGATGGAGACATGGTCACTGATTCAGTCTCTAGTGAGAGCTCTGTTGATAGTGACGATCCAGCTGACTCATCATGCCATCGTAGCCCTCAGGCAACTGACTATGACAATGGTCCTAGAACGGCTGCTGGCAGCCCTTGCAGTGTGATGCTCAAGGATCAGTCATCCACTGTTGCTCCAGTACTCTCAAGAGAGCCAAAAAAATCAGCTAACATTTCTTTCAGTAATCACGTTTCCCCTACATTGCCTAAATGGAGACTTTTAGGCAGCCATGTGCCAAACCTACAGGTTCCTTATAATGGAGCTTTGTGGAGTGCTCCCGACAGCTCATTGTCAAGTCCTTCTAGAAGCCCAGTGAGAGCATTTGGCACTGAGCAAGTCGTGAACTCTGCTTTCTGGGCTGCAAAAACTTATACAGATGTCACTCTACTTGGATCTGGCCACTGCTCCAGTCCAGGTTCGGGTCACAATTCTGGGCATAATTCAATGGGAGGGGATATGTCTGGACCGCTGTTTTGGCAACAAAGCAGGGGTAGCCCTGAGTATTCTCCGGTACCTAGTCCCAGAATGACAAGCCCAGGGCCAGGCTCAAGAATTCAGAGTGGTGCTGTTACGCCTATTCACCCTAGAGCAGGAGGGACTCCCAATGAGACACAGACAAGCTGGGCTGATGATGGGAAACAACAAAGTCACCGTTTACCTCTTCCCCCGACAATTACAATTGCTTCCCCTTTTTCTCATTCAAATTCAGCAGCAACATCTCCTTCTGTGCCACGAAGTCCGGGAAGGGCAGAGAACCCACCAAGCCCTGGTTCACGCTGGAAAAAGGGAAAGCTGCTGGGTAGAGGGACTTTTGGACATGTTTATGTTGGTTTTAATAGTGAAAATGGTGAAATGTGTGCGATGAAGGAGGTCACATTATTTTCTGATGATGCAAAGTCAAAGGAAAGCGCTAAGCAATTAAACCAGGAAATTGCTTTATTGAGCCGTTTACGGCACCCAAATATTGTGCAATATTATGGGTCTGAATCAGTTGGTGACCGACTATATATATATTTGGAGTATGTATCTGGTGGCTCCATATATAAACTTCTCCAAGACTATGGGCAATTTGGCGAACTAGCAATTCGTAGTTATACTAAGCAAATCTTGTCGGGGCTGGCATATTTACATGCTAAAAACACTGTTCACAGGGATATTAAAGGAGCAAATATACTTGTAGACCCAAATGGACGTGTCAAGTTGGCAGACTTTGGGATGGCCAAGCATATCACCGGGCAGTCATGTCCGTTATCATTTAAGGGAAGCCCTTACTGGATGGCACCTGAGGTTATAAAGAACTCAAGCGGTGCCAACCTTGCTGTGGATATATGGAGTCTTGGGTGCACTGTTTTGGAAATGGCTACGACAAAACCACCTTGGAGCCAGTATGAAGGGGTTGCTGCCATGTTTAAGATTGGGAATAGCAAAGAACTTCCAGCAATGCCAGATAGCCTCTTGGATGATGGAAAGGATTTCATAAGGCAATGCTTACAGAGAAATCCACTACATCGTCCTACAGCTGCCCAGCTTTTGGAGCATCCTTTTGTGAAATATGCTGCGCCTTTGGAAAGATCTATGTTGAGCACTGAGCCTTCTGATCCATCCTCTGGGATTACAAATGGAGTGAAAGCACTGGGTATTGGGCAAGGAAGGAATTTCTCCAACTTGGATTCAGATAGGCTTGCAATTCATTCATCTAGAGTTTCAAAAACTAATAACCATACCAGTGAGATCCATATTCCCAGGAACATATCATGCCCCGTCTCACCCATTGGAAGCCCTCTATTGCACTCAAGGTCACCACCACACCTAAATGGAAGAATGTCACCTTCACCTATATCTAGCCCACATACTACTTCGGGCTCATCCACTCCTCTTACAGGTGGCAGTGGTGCCATTCCATTTATTCACATGAACCAGTCCATCAACTTGCAAGAGGGTTTTGGAGGAATTTCAAAGCCCTCGAATGGTCTTTATGTCAATGGTCCTTCGTCCTATCATGATTCATGTCCTGACATGTTTCGAGGGAAGCAGCCAGGATCTCATATCTTCTCGGAACTGATACCCTGCGAAAATGATGTTCTGGGAAAGCAGTTTGTAAGGCATGCCCACGCAGAGCAGTATGATGGACAATCAGTTTTAGCTGATCGGGTGTCTCGCCAACTCCTTAAGGATCATGTTAAGATGAACCTTAAACTGGATTTAAGTCCCAACTCTCCTTTGCCGAACCGCACAAATGGTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010562 GO:0010604 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023052 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0097435 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

905

Amino Acids

97.45

Weight (kDa)

9.35

Isoelectric Point (pI)

62.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	417 - 672	6.7e-71	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	418 - 668	5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63700 AT1G63700
fragaria_vesca	FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_5g02670 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920 FvH4_7g29920
malus_domestica	MD01G1205500.v1.1 MD06G1085900.v1.1 MD07G1274900.v1.1
prunus_persica	Prupe.2G298100_v2.0.a1 Prupe.2G298100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1 Prupe.5G102100_v2.0.a1
pyrus_communis	pycom01g21500 pycom06g08220 pycom07g25100
rosa_chinensis	RchiOBHm_Chr1g0377881 RchiOBHm_Chr7g0197281
rosa_laevigata	RLG00000003944 RLG00000026489
rosa_multiflora	Rmu_co8459889.1_g000001 Rmu_sc0004491.1_g000001 Rmu_sc0004824.1_g000011 Rmu_sc0010019.1_g000003 Rmu_sc0017623.1_g000004 Rmu_sc0030897.1_g000001
rosa_roxburghii	Rroxscaffold_3G00258510 Rroxscaffold_4G00280980
rosa_rugosa	Rorug01G0405400 Rorug07G0039200
rosa_samantha	Rh1AG426400 Rh1BG382900 Rh1CG396700 Rh1DG413900 Rh7AG164200 Rh7BG166000 Rh7CG172700 Rh7DG165400
rosa_wichuraiana	Rw1G037210 Rw7G014320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	723, 1760
Acc36I	ACCTGC	1 cut(s)	400
Acc65I	GGTACC	1 cut(s)	979
AccB1I	GGYRCC	5 cut(s)	979, 1422, 1747, 1775, 2360
AccB7I	CCANNNNNTGG	4 cut(s)	281, 1235, 1999, 2075
AccI	GTMKAC	2 cut(s)	286, 1647
AciI	CCGC	3 cut(s)	935, 1773, 2698
AclWI	GGATC	8 cut(s)	148, 479, 591, 868, 2060, 2194, 2515, 2649
AcoI	YGGCCR	2 cut(s)	865, 1686
AcsI	RAATTY	6 cut(s)	95, 313, 1020, 1171, 2122, 2418
AcuI	CTGAAG	1 cut(s)	93
AfaI	GTAC	3 cut(s)	310, 609, 981
AfeI	AGCGCT	1 cut(s)	1378
AflII	CTTAAG	1 cut(s)	2636
AflIII	ACRYGT	3 cut(s)	1285, 1660, 2484
AgsI	TTSAA	4 cut(s)	912, 1170, 2175, 2424
AjiI	CACGTC	1 cut(s)	1661
AjnI	CCWGG	8 cut(s)	280, 880, 1003, 1009, 1234, 1392, 2210, 2503
AjuI	GAANNNNNNNTTGG	2 cut(s)	1222, 1254
Alw21I	GWGCWC	4 cut(s)	470, 745, 1814, 2054
Alw26I	GTCTC	7 cut(s)	382, 429, 460, 676, 1070, 2236, 2628
Alw44I	GTGCAC	1 cut(s)	1810
AlwI	GGATC	8 cut(s)	148, 479, 591, 868, 2060, 2194, 2515, 2649
Ama87I	CYCGRG	1 cut(s)	385
Aor51HI	AGCGCT	1 cut(s)	1378
AoxI	GGCC	3 cut(s)	865, 1007, 1686
ApaLI	GTGCAC	1 cut(s)	1810
ApoI	RAATTY	6 cut(s)	95, 313, 1020, 1171, 2122, 2418
ArsI	GACNNNNNNTTYG	2 cut(s)	924, 956
Asp718I	GGTACC	1 cut(s)	979
AspLEI	GCGC	2 cut(s)	1379, 2029
AspS9I	GGNCC	4 cut(s)	539, 932, 1007, 2453
AsuC2I	CCSGG	2 cut(s)	1209, 1702
AvaI	CYCGRG	1 cut(s)	385
AvaII	GGWCC	3 cut(s)	539, 932, 2453
AxyI	CCTNAGG	2 cut(s)	516, 1752
BaeGI	GKGCMC	1 cut(s)	1814
BalI	TGGCCA	2 cut(s)	867, 1688
BanI	GGYRCC	5 cut(s)	979, 1422, 1747, 1775, 2360
BanII	GRGCYC	2 cut(s)	470, 2333
Bbv12I	GWGCWC	4 cut(s)	470, 745, 1814, 2054
BccI	CCATC	9 cut(s)	425, 513, 1094, 1678, 1738, 1925, 2077, 2399, 2588
BceAI	ACGGC	3 cut(s)	564, 1398, 1436
BcgI	CGANNNNNNTGC	2 cut(s)	135, 169
BciT130I	CCWGG	8 cut(s)	282, 882, 1005, 1011, 1236, 1394, 2212, 2505
BcnI	CCSGG	2 cut(s)	1209, 1702
BcoDI	GTCTC	7 cut(s)	382, 429, 460, 676, 1070, 2236, 2628
BfaI	CTAG	8 cut(s)	459, 543, 771, 984, 1053, 1547, 2166, 2310
BfmI	CTRYAG	3 cut(s)	192, 710, 1983
BfoI	RGCGCY	1 cut(s)	1380
BfrI	CTTAAG	1 cut(s)	2636
BfuAI	ACCTGC	1 cut(s)	400
BglII	AGATCT	2 cut(s)	203, 2039
BlpI	GCTNAGC	2 cut(s)	264, 1380
BmcAI	AGTACT	1 cut(s)	609
Bme18I	GGWCC	3 cut(s)	539, 932, 2453
BmeT110I	CYCGRG	1 cut(s)	385
BmgBI	CACGTC	1 cut(s)	1661
BmgT120I	GGNCC	4 cut(s)	539, 932, 1007, 2453
BmiI	GGNNCC	8 cut(s)	717, 981, 1424, 1504, 1749, 1777, 1850, 2362
BmrI	ACTGGG	2 cut(s)	773, 2111
BmsI	GCATC	4 cut(s)	408, 564, 1348, 2014
BmuI	ACTGGG	2 cut(s)	773, 2111
BoxI	GACNNNNGTC	1 cut(s)	537
BpmI	CTGGAG	2 cut(s)	588, 859
Bpu1102I	GCTNAGC	2 cut(s)	264, 1380
BpuEI	CTTGAG	5 cut(s)	563, 598, 1000, 1753, 2245
BpuMI	CCSGG	2 cut(s)	1209, 1702
BsaBI	GATNNNNATC	1 cut(s)	184
BsaI	GGTCTC	1 cut(s)	382
BsaJI	CCNNGG	5 cut(s)	1003, 1004, 1234, 1844, 2210
BsaWI	WCCGGW	1 cut(s)	976
BsaXI	ACNNNNNCTCC	4 cut(s)	426, 456, 586, 616
Bse1I	ACTGG	9 cut(s)	605, 779, 876, 1745, 1852, 2106, 2193, 2386, 2670
Bse21I	CCTNAGG	2 cut(s)	516, 1752
Bse3DI	GCAATG	4 cut(s)	163, 668, 1914, 1958
Bse8I	GATNNNNATC	1 cut(s)	184
BseBI	CCWGG	8 cut(s)	282, 882, 1005, 1011, 1236, 1394, 2212, 2505
BseDI	CCNNGG	5 cut(s)	1003, 1004, 1234, 1844, 2210
BseGI	GGATG	9 cut(s)	185, 289, 590, 1689, 1749, 1933, 2005, 2069, 2333
BseJI	GATNNNNATC	1 cut(s)	184
BseMI	GCAATG	4 cut(s)	163, 668, 1914, 1958
BseMII	CTCAG	6 cut(s)	278, 530, 789, 957, 1743, 2046
BseNI	ACTGG	9 cut(s)	605, 779, 876, 1745, 1852, 2106, 2193, 2386, 2670
BseRI	GAGGAG	1 cut(s)	2331
BseSI	GKGCMC	1 cut(s)	1814
BseYI	CCCAGC	3 cut(s)	1089, 1264, 1992
BshFI	GGCC	3 cut(s)	867, 1009, 1688
BshNI	GGYRCC	5 cut(s)	979, 1422, 1747, 1775, 2360
BsiHKAI	GWGCWC	4 cut(s)	470, 745, 1814, 2054
BsiHKCI	CYCGRG	1 cut(s)	385
BsiSI	CCGG	3 cut(s)	977, 1208, 1701
BslFI	GGGAC	4 cut(s)	972, 1078, 1288, 2661
BsmAI	GTCTC	7 cut(s)	382, 429, 460, 676, 1070, 2236, 2628
BsmBI	CGTCTC	1 cut(s)	2236
BsmFI	GGGAC	4 cut(s)	972, 1078, 1288, 2661
BsnI	GGCC	3 cut(s)	867, 1009, 1688
Bso31I	GGTCTC	1 cut(s)	382
BsoBI	CYCGRG	1 cut(s)	385
Bsp1286I	GDGCHC	5 cut(s)	470, 745, 1814, 2054, 2333
Bsp1720I	GCTNAGC	2 cut(s)	264, 1380
BspACI	CCGC	3 cut(s)	935, 1773, 2698
BspANI	GGCC	3 cut(s)	867, 1009, 1688
BspCNI	CTCAG	6 cut(s)	277, 529, 790, 958, 1744, 2047
BspHI	TCATGA	1 cut(s)	2467
BspLI	GGNNCC	8 cut(s)	717, 981, 1424, 1504, 1749, 1777, 1850, 2362
BspMI	ACCTGC	1 cut(s)	400
BspPI	GGATC	8 cut(s)	148, 479, 591, 868, 2060, 2194, 2515, 2649
BspT107I	GGYRCC	5 cut(s)	979, 1422, 1747, 1775, 2360
BspTI	CTTAAG	1 cut(s)	2636
BspTNI	GGTCTC	1 cut(s)	382
BsrDI	GCAATG	4 cut(s)	163, 668, 1914, 1958
BsrI	ACTGG	9 cut(s)	605, 779, 876, 1745, 1852, 2106, 2193, 2386, 2670
BssECI	CCNNGG	5 cut(s)	1003, 1004, 1234, 1844, 2210
BssT1I	CCWWGG	1 cut(s)	1844
Bst2UI	CCWGG	8 cut(s)	282, 882, 1005, 1011, 1236, 1394, 2212, 2505
Bst4CI	ACNGT	4 cut(s)	598, 1121, 1612, 1816
Bst6I	CTCTTC	1 cut(s)	1134
BstAFI	CTTAAG	1 cut(s)	2636
BstAPI	GCANNNNNTGC	1 cut(s)	564
BstC8I	GCNNGC	5 cut(s)	397, 556, 1587, 2151, 2574
BstDEI	CTNAG	9 cut(s)	264, 360, 516, 798, 966, 1380, 1566, 1752, 2055
BstEII	GGTNACC	2 cut(s)	1463, 2265
BstENI	CCTNNNNNAGG	2 cut(s)	1056, 2346
BstF5I	GGATG	9 cut(s)	185, 289, 590, 1689, 1749, 1933, 2005, 2069, 2333
BstH2I	RGCGCY	1 cut(s)	1380
BstHHI	GCGC	2 cut(s)	1379, 2029
BstMAI	GTCTC	7 cut(s)	382, 429, 460, 676, 1070, 2236, 2628
BstMWI	GCNNNNNNNGC	8 cut(s)	302, 510, 564, 824, 943, 960, 1784, 2002
BstNI	CCWGG	8 cut(s)	282, 882, 1005, 1011, 1236, 1394, 2212, 2505
BstNSI	RCATGY	4 cut(s)	1289, 1601, 2488, 2576
BstPAI	GACNNNNGTC	1 cut(s)	537
BstPI	GGTNACC	2 cut(s)	1463, 2265
BstSFI	CTRYAG	3 cut(s)	192, 710, 1983
BstSLI	GKGCMC	1 cut(s)	1814
BstX2I	RGATCY	6 cut(s)	140, 203, 860, 2039, 2199, 2507
BstXI	CCANNNNNNTGG	1 cut(s)	1529
BstYI	RGATCY	6 cut(s)	140, 203, 860, 2039, 2199, 2507
Bsu36I	CCTNAGG	2 cut(s)	516, 1752
BsuRI	GGCC	3 cut(s)	867, 1009, 1688
BtgZI	GCGATG	1 cut(s)	1343
BtrI	CACGTC	1 cut(s)	1661
BtsCI	GGATG	9 cut(s)	185, 289, 590, 1689, 1749, 1933, 2005, 2069, 2333
BtsI	GCAGTG	3 cut(s)	574, 868, 2362
BveI	ACCTGC	1 cut(s)	400
Cac8I	GCNNGC	5 cut(s)	397, 556, 1587, 2151, 2574
CciI	TCATGA	1 cut(s)	2467
CfoI	GCGC	2 cut(s)	1379, 2029
Cfr13I	GGNCC	4 cut(s)	539, 932, 1007, 2453
Csp6I	GTAC	3 cut(s)	309, 608, 980
CviQI	GTAC	3 cut(s)	309, 608, 980
DdeI	CTNAG	9 cut(s)	264, 360, 516, 798, 966, 1380, 1566, 1752, 2055
EaeI	YGGCCR	2 cut(s)	865, 1686
Eam1104I	CTCTTC	1 cut(s)	1134
EarI	CTCTTC	1 cut(s)	1134
Ecl136II	GAGCTC	1 cut(s)	468
Eco130I	CCWWGG	1 cut(s)	1844
Eco24I	GRGCYC	2 cut(s)	470, 2333
Eco31I	GGTCTC	1 cut(s)	382
Eco47I	GGWCC	3 cut(s)	539, 932, 2453
Eco47III	AGCGCT	1 cut(s)	1378
Eco53kI	GAGCTC	1 cut(s)	468
Eco57I	CTGAAG	1 cut(s)	93
Eco81I	CCTNAGG	2 cut(s)	516, 1752
Eco88I	CYCGRG	1 cut(s)	385
Eco91I	GGTNACC	2 cut(s)	1463, 2265
EcoICRI	GAGCTC	1 cut(s)	468
EcoNI	CCTNNNNNAGG	2 cut(s)	1056, 2346
EcoO65I	GGTNACC	2 cut(s)	1463, 2265
EcoRI	GAATTC	1 cut(s)	1020
EcoRII	CCWGG	8 cut(s)	280, 880, 1003, 1009, 1234, 1392, 2210, 2503
EcoT14I	CCWWGG	1 cut(s)	1844
EcoT38I	GRGCYC	2 cut(s)	470, 2333
ErhI	CCWWGG	1 cut(s)	1844
Esp3I	CGTCTC	1 cut(s)	2236
FalI	AAGNNNNNCTT	4 cut(s)	193, 225, 1559, 1591
FaqI	GGGAC	4 cut(s)	972, 1078, 1288, 2661
FblI	GTMKAC	2 cut(s)	286, 1647
FokI	GGATG	9 cut(s)	192, 276, 577, 1696, 1756, 1940, 1992, 2056, 2320
FriOI	GRGCYC	2 cut(s)	470, 2333
FspBI	CTAG	8 cut(s)	459, 543, 771, 984, 1053, 1547, 2166, 2310
GlaI	GCGC	2 cut(s)	1378, 2028
GsaI	CCCAGC	3 cut(s)	1093, 1268, 1996
GsuI	CTGGAG	2 cut(s)	588, 859
HaeII	RGCGCY	1 cut(s)	1380
HaeIII	GGCC	3 cut(s)	867, 1009, 1688
HapII	CCGG	3 cut(s)	977, 1208, 1701
HhaI	GCGC	2 cut(s)	1379, 2029
Hin6I	GCGC	2 cut(s)	1377, 2027
HinP1I	GCGC	2 cut(s)	1377, 2027
HinfI	GANTC	9 cut(s)	80, 197, 449, 494, 1066, 1454, 1801, 2138, 2471
HpaII	CCGG	3 cut(s)	977, 1208, 1701
Hpy166II	GTNNAC	8 cut(s)	287, 811, 1124, 1241, 1418, 1615, 1648, 1812
Hpy8I	GTNNAC	8 cut(s)	287, 811, 1124, 1241, 1418, 1615, 1648, 1812
Hpy99I	CGWCG	1 cut(s)	37
HpyCH4III	ACNGT	4 cut(s)	598, 1121, 1612, 1816
HpyCH4IV	ACGT	3 cut(s)	426, 657, 1660
HpyF10VI	GCNNNNNNNGC	8 cut(s)	302, 510, 564, 824, 943, 960, 1784, 2002
HpyF3I	CTNAG	9 cut(s)	264, 360, 516, 798, 966, 1380, 1566, 1752, 2055
HpySE526I	ACGT	3 cut(s)	426, 657, 1660
HspAI	GCGC	2 cut(s)	1377, 2027
KpnI	GGTACC	1 cut(s)	983
LmnI	GCTCC	8 cut(s)	607, 728, 748, 878, 1508, 1631, 1848, 2002
LweI	GCATC	4 cut(s)	408, 564, 1348, 2014
MaeI	CTAG	8 cut(s)	459, 543, 771, 984, 1053, 1547, 2166, 2310
MaeII	ACGT	3 cut(s)	426, 657, 1660
MboII	GAAGA	6 cut(s)	64, 99, 159, 1121, 2298, 2509
MfeI	CAATTG	1 cut(s)	1146
MflI	RGATCY	6 cut(s)	140, 203, 860, 2039, 2199, 2507
MhlI	GDGCHC	5 cut(s)	470, 745, 1814, 2054, 2333
MlsI	TGGCCA	2 cut(s)	867, 1688
MluNI	TGGCCA	2 cut(s)	867, 1688
MlyI	GAGTC	4 cut(s)	74, 488, 1060, 1810
MmeI	TCCRAC	1 cut(s)	2154
Mox20I	TGGCCA	2 cut(s)	867, 1688
MscI	TGGCCA	2 cut(s)	867, 1688
MslI	CAYNNNNRTG	3 cut(s)	1290, 2193, 2280
Msp20I	TGGCCA	2 cut(s)	867, 1688
MspA1I	CMGCKG	3 cut(s)	491, 937, 1988
MspCI	CTTAAG	1 cut(s)	2636
MspI	CCGG	3 cut(s)	977, 1208, 1701
MunI	CAATTG	1 cut(s)	1146
MvaI	CCWGG	8 cut(s)	282, 882, 1005, 1011, 1236, 1394, 2212, 2505
MwoI	GCNNNNNNNGC	8 cut(s)	302, 510, 564, 824, 943, 960, 1784, 2002
NciI	CCSGG	2 cut(s)	1209, 1702
NlaIV	GGNNCC	8 cut(s)	717, 981, 1424, 1504, 1749, 1777, 1850, 2362
NmuCI	GTSAC	9 cut(s)	442, 479, 847, 890, 1115, 1339, 1463, 2265, 2292
NspI	RCATGY	4 cut(s)	1289, 1601, 2488, 2576
PaeI	GCATGC	1 cut(s)	2576
PagI	TCATGA	1 cut(s)	2467
PasI	CCCWGGG	1 cut(s)	1004
PciI	ACATGT	2 cut(s)	1285, 2484
PfeI	GAWTC	5 cut(s)	197, 449, 1454, 2138, 2471
PflFI	GACNNNGTC	1 cut(s)	440
PflMI	CCANNNNNTGG	4 cut(s)	281, 1235, 1999, 2075
PfoI	TCCNGGA	1 cut(s)	1207
PleI	GAGTC	4 cut(s)	74, 488, 1060, 1809
PpsI	GAGTC	4 cut(s)	74, 488, 1060, 1809
PscI	ACATGT	2 cut(s)	1285, 2484
PshAI	GACNNNNGTC	1 cut(s)	537
PsiI	TTATAA	2 cut(s)	723, 1760
Psp124BI	GAGCTC	1 cut(s)	470
Psp6I	CCWGG	8 cut(s)	280, 880, 1003, 1009, 1234, 1392, 2210, 2503
PspEI	GGTNACC	2 cut(s)	1463, 2265
PspFI	CCCAGC	3 cut(s)	1089, 1264, 1992
PspGI	CCWGG	8 cut(s)	280, 880, 1003, 1009, 1234, 1392, 2210, 2503
PspN4I	GGNNCC	8 cut(s)	717, 981, 1424, 1504, 1749, 1777, 1850, 2362
PspPI	GGNCC	4 cut(s)	539, 932, 1007, 2453
PsuI	RGATCY	6 cut(s)	140, 203, 860, 2039, 2199, 2507
PsyI	GACNNNGTC	1 cut(s)	440
PvuII	CAGCTG	2 cut(s)	491, 1988
RsaI	GTAC	3 cut(s)	310, 609, 981
RsaNI	GTAC	3 cut(s)	309, 608, 980
RseI	CAYNNNNRTG	3 cut(s)	1290, 2193, 2280
SacI	GAGCTC	1 cut(s)	470
Sau96I	GGNCC	4 cut(s)	539, 932, 1007, 2453
ScaI	AGTACT	1 cut(s)	609
SchI	GAGTC	4 cut(s)	74, 488, 1060, 1810
SduI	GDGCHC	5 cut(s)	470, 745, 1814, 2054, 2333
SfaNI	GCATC	4 cut(s)	408, 564, 1348, 2014
SfcI	CTRYAG	3 cut(s)	192, 710, 1983
SinI	GGWCC	3 cut(s)	539, 932, 2453
SmiMI	CAYNNNNRTG	3 cut(s)	1290, 2193, 2280
SmlI	CTYRAG	6 cut(s)	578, 613, 1015, 1768, 2260, 2636
SmoI	CTYRAG	6 cut(s)	578, 613, 1015, 1768, 2260, 2636
SphI	GCATGC	1 cut(s)	2576
SsiI	CCGC	3 cut(s)	935, 1773, 2698
SspI	AATATT	2 cut(s)	1432, 1442
SspMI	CTAG	8 cut(s)	459, 543, 771, 984, 1053, 1547, 2166, 2310
SstI	GAGCTC	1 cut(s)	470
StyI	CCWWGG	1 cut(s)	1844
TaaI	ACNGT	4 cut(s)	598, 1121, 1612, 1816
TaiI	ACGT	3 cut(s)	429, 660, 1663
TaqI	TCGA	3 cut(s)	145, 2432, 2491
TaqII	GACCGA	1 cut(s)	1482
TatI	WGTACW	1 cut(s)	607
TfiI	GAWTC	5 cut(s)	197, 449, 1454, 2138, 2471
TseFI	GTSAC	9 cut(s)	442, 479, 847, 890, 1115, 1339, 1463, 2265, 2292
Tsp45I	GTSAC	9 cut(s)	442, 479, 847, 890, 1115, 1339, 1463, 2265, 2292
TspGWI	ACGGA	1 cut(s)	1704
Tth111I	GACNNNGTC	1 cut(s)	440
Van91I	CCANNNNNTGG	4 cut(s)	281, 1235, 1999, 2075
Vha464I	CTTAAG	1 cut(s)	2636
VneI	GTGCAC	1 cut(s)	1810
VpaK11BI	GGWCC	3 cut(s)	539, 932, 2453
XagI	CCTNNNNNAGG	2 cut(s)	1056, 2346
XapI	RAATTY	6 cut(s)	95, 313, 1020, 1171, 2122, 2418
XbaI	TCTAGA	2 cut(s)	770, 2165
XceI	RCATGY	4 cut(s)	1289, 1601, 2488, 2576
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1786
XmiI	GTMKAC	2 cut(s)	286, 1647
XspI	CTAG	8 cut(s)	459, 543, 771, 984, 1053, 1547, 2166, 2310
ZrmI	AGTACT	1 cut(s)	609

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.