MD01G1219000.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003088
Physical Location & Seq
Forward (+)
31126727 .. 31127715
989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1219000.v1.1.491

Sequence Viewer

Length: 690 bp
ATGCTTGTTTTGGGTTTGTGCAAGTACAACTTGAAAGATGCAGTCCTTGAAGGAGGTATCCCATTCAACAAGGCCTACGGAATGACTGCTTTTGAGTACCATGGCACTGACCCCAGATTCAACAAGGGAATGGCTAACCACTCTACCATTACCATGAAGAACCTTCTTGAGAACTACAATGGCTTTGAGGGCCTCACATCCATCGTTGATGTTGGTGGTGGCACCGGCATTGTCCTTAACATGATCGTCTTTAAATACCCTTCGATTAAGGGCATTAACTTCGACCTGCCTTATGTCATCGAAGATGCTCCCCAATATCCTGGTGTGGAGCATGTTGGAGGAGACATGTTTGTGAGTGTTCCAAAGGGAGATGCAATTTTTATGAAGTGGATATGCCACGACTGGAGTGACGAGCATTGCTTGAAATTTTTGAAGAACTGCTATTCTGCGCTCCCAGACAATGGGAAGGTAATTGTTGCTGAGTGCATTCTTCCGGTAGCTCCGGACAGTAGCCTTGCCACCAAGGGAGTTGTCCATATCGACGCGATCATGTTAGCTCACAACCCCGGCGGAAAAGAGAGGACGGAGAAGGAGTTCGAGGCCTTGGCTAAGGGATCTGGATTCCAAGGCTTTCGAGTCGTGTGCTCTGCTTTCAACACCTATGCCATTGAATTTCTTAAGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

230

Amino Acids

25.11

Weight (kDa)

5.97

Isoelectric Point (pI)

25.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 10 - 209 1.6e-71 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 294
AccB1I GGYRCC 1 cut(s) 221
AccB7I CCANNNNNTGG 1 cut(s) 461
AccII CGCG 1 cut(s) 545
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 1 cut(s) 570
AclWI GGATC 1 cut(s) 622
AcsI RAATTY 2 cut(s) 425, 671
AfaI GTAC 2 cut(s) 26, 98
AfiI CCNNNNNNNGG 1 cut(s) 461
AflII CTTAAG 1 cut(s) 677
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 8 cut(s) 34, 50, 67, 121, 424, 433, 655, 671
AjnI CCWGG 1 cut(s) 319
AloI GAACNNNNNNTCC 2 cut(s) 578, 610
AluBI AGCT 2 cut(s) 500, 557
AluI AGCT 2 cut(s) 500, 557
Alw21I GWGCWC 1 cut(s) 647
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 1 cut(s) 622
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 3 cut(s) 72, 190, 600
ApoI RAATTY 2 cut(s) 425, 671
Asp700I GAANNNNTTC 1 cut(s) 593
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 1 cut(s) 190
AsuC2I CCSGG 1 cut(s) 567
BanI GGYRCC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 647
BccI CCATC 1 cut(s) 209
BcgI CGANNNNNNTGC 4 cut(s) 262, 296, 624, 658
BciT130I CCWGG 1 cut(s) 321
BciVI GTATCC 1 cut(s) 68
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 336
BfrI CTTAAG 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 294
BfuI GTATCC 1 cut(s) 68
Bme1390I CCNGG 2 cut(s) 321, 567
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 2 cut(s) 321, 567
BmsI GCATC 3 cut(s) 28, 295, 361
BpmI CTGGAG 1 cut(s) 424
Bpu10I CCTNAGC 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 567
BsaJI CCNNGG 5 cut(s) 100, 522, 565, 603, 625
BsaWI WCCGGW 2 cut(s) 493, 502
BsaXI ACNNNNNCTCC 2 cut(s) 578, 608
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 415
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 5 cut(s) 100, 522, 565, 603, 625
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 1 cut(s) 407
BseRI GAGGAG 1 cut(s) 354
Bsh1236I CGCG 1 cut(s) 545
BshFI GGCC 3 cut(s) 74, 192, 602
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 647
BsiSI CCGG 4 cut(s) 225, 494, 503, 567
BslI CCNNNNNNNGG 1 cut(s) 461
BsmAI GTCTC 1 cut(s) 336
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 3 cut(s) 74, 192, 602
Bsp1286I GDGCHC 1 cut(s) 647
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 3 cut(s) 243, 546, 614
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 1 cut(s) 570
BspANI GGCC 3 cut(s) 74, 192, 602
BspCNI CTCAG 1 cut(s) 472
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 223
BspMI ACCTGC 1 cut(s) 294
BspPI GGATC 1 cut(s) 622
BspT107I GGYRCC 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 677
BsrDI GCAATG 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 224
BssECI CCNNGG 5 cut(s) 100, 522, 565, 603, 625
BssMI GATC 3 cut(s) 243, 546, 614
BssT1I CCWWGG 4 cut(s) 100, 522, 603, 625
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 509
BstAFI CTTAAG 1 cut(s) 677
BstDEI CTNAG 2 cut(s) 480, 609
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 545
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 3 cut(s) 246, 549, 617
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 3 cut(s) 243, 546, 614
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 2 cut(s) 335, 349
BstSCI CCNGG 2 cut(s) 319, 565
BstUI CGCG 1 cut(s) 545
BstX2I RGATCY 1 cut(s) 614
BstXI CCANNNNNNTGG 1 cut(s) 320
BstYI RGATCY 1 cut(s) 614
BsuI GTATCC 1 cut(s) 68
BsuRI GGCC 3 cut(s) 74, 192, 602
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 294
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 1 cut(s) 190
CseI GACGC 1 cut(s) 551
Csp6I GTAC 2 cut(s) 25, 97
CviAII CATG 6 cut(s) 101, 154, 241, 332, 346, 550
CviQI GTAC 2 cut(s) 25, 97
DdeI CTNAG 2 cut(s) 480, 609
DpnI GATC 3 cut(s) 245, 548, 616
DpnII GATC 3 cut(s) 243, 546, 614
DraI TTTAAA 1 cut(s) 253
EciI GGCGGA 1 cut(s) 585
Eco130I CCWWGG 4 cut(s) 100, 522, 603, 625
Eco147I AGGCCT 2 cut(s) 74, 602
EcoO109I RGGNCCY 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 4 cut(s) 100, 522, 603, 625
ErhI CCWWGG 4 cut(s) 100, 522, 603, 625
FaeI CATG 6 cut(s) 104, 157, 244, 335, 349, 553
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 6 cut(s) 100, 153, 240, 331, 345, 549
FokI GGATG 1 cut(s) 184
GlaI GCGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 424
HaeIII GGCC 3 cut(s) 74, 192, 602
HapII CCGG 4 cut(s) 225, 494, 503, 567
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 451
Hin1II CATG 6 cut(s) 104, 157, 244, 335, 349, 553
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HinfI GANTC 3 cut(s) 117, 621, 636
HpaII CCGG 4 cut(s) 225, 494, 503, 567
Hpy188III TCNNGA 3 cut(s) 167, 503, 618
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 5 cut(s) 44, 173, 270, 460, 583
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4V TGCA 4 cut(s) 21, 41, 374, 486
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 2 cut(s) 480, 609
Hsp92II CATG 6 cut(s) 104, 157, 244, 335, 349, 553
HspAI GCGC 1 cut(s) 449
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 3 cut(s) 243, 546, 614
LmnI GCTCC 4 cut(s) 313, 328, 456, 505
LweI GCATC 3 cut(s) 28, 295, 361
MaeIII GTNAC 1 cut(s) 407
MalI GATC 3 cut(s) 245, 548, 616
MboI GATC 3 cut(s) 243, 546, 614
MboII GAAGA 4 cut(s) 169, 314, 445, 482
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 1 cut(s) 647
MluCI AATT 5 cut(s) 375, 425, 471, 671, 685
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 6 cut(s) 47, 181, 203, 332, 573, 592
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 593
MseI TTAA 5 cut(s) 237, 252, 267, 276, 678
MslI CAYNNNNRTG 2 cut(s) 152, 350
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 4 cut(s) 225, 494, 503, 567
MspR9I CCNGG 2 cut(s) 321, 567
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 321
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 189
NciI CCSGG 1 cut(s) 567
NcoI CCATGG 1 cut(s) 100
NdeII GATC 3 cut(s) 243, 546, 614
NlaIII CATG 6 cut(s) 104, 157, 244, 335, 349, 553
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 407
NspI RCATGY 2 cut(s) 335, 349
PceI AGGCCT 2 cut(s) 74, 602
PciI ACATGT 1 cut(s) 345
PctI GAATGC 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 593
PfeI GAWTC 2 cut(s) 117, 621
PflMI CCANNNNNTGG 1 cut(s) 461
PleI GAGTC 1 cut(s) 644
PpsI GAGTC 1 cut(s) 644
PscI ACATGT 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 190
PsuI RGATCY 1 cut(s) 614
RsaI GTAC 2 cut(s) 26, 98
RsaNI GTAC 2 cut(s) 25, 97
RseI CAYNNNNRTG 2 cut(s) 152, 350
SaqAI TTAA 5 cut(s) 237, 252, 267, 276, 678
Sau3AI GATC 3 cut(s) 243, 546, 614
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 2 cut(s) 321, 567
SduI GDGCHC 1 cut(s) 647
SetI ASST 7 cut(s) 58, 165, 288, 471, 502, 559, 662
SfaNI GCATC 3 cut(s) 28, 295, 361
SmiMI CAYNNNNRTG 2 cut(s) 152, 350
SmlI CTYRAG 2 cut(s) 167, 677
SmoI CTYRAG 2 cut(s) 167, 677
Sse9I AATT 5 cut(s) 375, 425, 471, 671, 685
SseBI AGGCCT 2 cut(s) 74, 602
SsiI CCGC 1 cut(s) 570
StuI AGGCCT 2 cut(s) 74, 602
StyD4I CCNGG 2 cut(s) 319, 565
StyI CCWWGG 4 cut(s) 100, 522, 603, 625
TaaI ACNGT 1 cut(s) 509
TaqI TCGA 6 cut(s) 263, 282, 300, 540, 597, 634
TasI AATT 5 cut(s) 375, 425, 471, 671, 685
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 2 cut(s) 117, 621
Tru1I TTAA 5 cut(s) 237, 252, 267, 276, 678
Tru9I TTAA 5 cut(s) 237, 252, 267, 276, 678
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 2 cut(s) 170, 398
TspGWI ACGGA 2 cut(s) 93, 599
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 677
XapI RAATTY 2 cut(s) 425, 671
XceI RCATGY 2 cut(s) 335, 349
XmnI GAANNNNTTC 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.