MD07G1301000.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003088
Physical Location & Seq
Reverse (-)
35977894 .. 35978511
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1301000.v1.1.491

Sequence Viewer

Length: 243 bp
ATGCTTGGTATCAACTTCGACTTGCCTCATGTCATAGAAGATGCTCCCAAATTTCTTGGTGTGGAGCATGTTGGAGGAGACATGTTTGCAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGAACTGTTATACCGCACTCCCTGACAATGGGAAGGAGATTCTTGCTGAAGGCATTCTTCCAGTAGCTGCAGACAGCAGCCTGGCCACCAAGGGAGTTGTCAATCTCGACGTGATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

81

Amino Acids

8.44

Weight (kDa)

4.46

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 3 - 41 2.1e-09 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 135
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 81
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 72
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 188, 198
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 174
BalI TGGCCA 1 cut(s) 206
BbvI GCAGC 2 cut(s) 175, 210
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 72
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 2 cut(s) 189, 199
BlsI GCNGC 2 cut(s) 190, 200
Bme1390I CCNGG 1 cut(s) 203
BmgBI CACGTC 1 cut(s) 232
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 2 cut(s) 31, 97
BsaJI CCNNGG 1 cut(s) 210
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 182
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 2 cut(s) 175, 210
BshFI GGCC 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 1 cut(s) 206
Bsp143I GATC 1 cut(s) 234
BspACI CCGC 1 cut(s) 135
BspANI GGCC 1 cut(s) 206
BspHI TCATGA 1 cut(s) 117
BspMAI CTGCAG 1 cut(s) 193
BsrI ACTGG 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 210
BssMI GATC 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 128
BstKTI GATC 1 cut(s) 237
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 71, 85
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 175, 210
BsuRI GGCC 1 cut(s) 206
BtrI CACGTC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 117
CviAII CATG 5 cut(s) 29, 68, 82, 118, 238
CviJI RGCY 3 cut(s) 188, 201, 206
CviKI_1 RGCY 3 cut(s) 188, 201, 206
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 5 cut(s) 32, 71, 85, 121, 241
FaiI YATR 7 cut(s) 30, 35, 69, 83, 119, 132, 239
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FatI CATG 5 cut(s) 28, 67, 81, 117, 237
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 2 cut(s) 189, 199
Fsp4HI GCNGC 2 cut(s) 189, 199
GluI GCNGC 2 cut(s) 189, 199
HaeIII GGCC 1 cut(s) 206
Hin1II CATG 5 cut(s) 32, 71, 85, 121, 241
HinfI GANTC 1 cut(s) 160
Hpy188III TCNNGA 2 cut(s) 118, 227
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 2 cut(s) 148, 164
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 89, 110, 191
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 5 cut(s) 32, 71, 85, 121, 241
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 1 cut(s) 234
LmnI GCTCC 2 cut(s) 49, 64
LpnPI CCDG 4 cut(s) 156, 188, 195, 215
Lsp1109I GCAGC 2 cut(s) 175, 210
LweI GCATC 2 cut(s) 31, 97
MaeII ACGT 1 cut(s) 231
MalI GATC 1 cut(s) 236
MboI GATC 1 cut(s) 234
MboII GAAGA 3 cut(s) 50, 133, 170
MlsI TGGCCA 1 cut(s) 206
MluCI AATT 2 cut(s) 50, 111
MluNI TGGCCA 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 2 cut(s) 36, 68
Mox20I TGGCCA 1 cut(s) 206
MroXI GAANNNNTTC 1 cut(s) 174
MscI TGGCCA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 1 cut(s) 203
NdeII GATC 1 cut(s) 234
NlaIII CATG 5 cut(s) 32, 71, 85, 121, 241
NspI RCATGY 2 cut(s) 71, 85
PagI TCATGA 1 cut(s) 117
PciI ACATGT 1 cut(s) 81
PctI GAATGC 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 2 cut(s) 190, 200
PscI ACATGT 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PstI CTGCAG 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 188
SatI GCNGC 2 cut(s) 189, 199
Sau3AI GATC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 203
SetI ASST 2 cut(s) 190, 234
SfaNI GCATC 2 cut(s) 31, 97
SfcI CTRYAG 1 cut(s) 189
Sse9I AATT 2 cut(s) 50, 111
SsiI CCGC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 128
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 18, 228
TasI AATT 2 cut(s) 50, 111
TfiI GAWTC 1 cut(s) 160
TseI GCWGC 2 cut(s) 188, 198
TspDTI ATGAA 2 cut(s) 106, 134
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 71, 85
XmnI GAANNNNTTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.