MD07G1209700.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003088
Physical Location & Seq
Reverse (-)
28883996 .. 28884205
210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1209700.v1.1.491

Sequence Viewer

Length: 210 bp
ATGTGCTGTCTTCCAGTAGCTCCGGACAGCAGCCTTGCCATCAAGGGAGTTGTCCATATCGACGTGATCTTGTTAGCTCACAACCCCGGCGGAAAAGAGAGGACGGAAAAGGAGTTTGAGGCCTTGGCTAAGGGATCTGGATACCAAGGCTTCCGAGTCATCCGCTCCGCTTTCAACACCTATGTCATTGAGTTTCTTAAGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

70

Amino Acids

7.58

Weight (kDa)

8.69

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 165
AccIII TCCGGA 1 cut(s) 22
AciI CCGC 3 cut(s) 90, 163, 168
AclWI GGATC 1 cut(s) 142
AflII CTTAAG 1 cut(s) 197
AgsI TTSAA 1 cut(s) 175
AjiI CACGTC 1 cut(s) 64
AluBI AGCT 2 cut(s) 20, 77
AluI AGCT 2 cut(s) 20, 77
AlwI GGATC 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 22
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 1 cut(s) 30
AsuC2I CCSGG 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 2
BbvI GCAGC 1 cut(s) 42
BccI CCATC 1 cut(s) 47
BciVI GTATCC 1 cut(s) 134
BcnI CCSGG 1 cut(s) 87
BfrI CTTAAG 1 cut(s) 197
BfuI GTATCC 1 cut(s) 134
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 64
BmrFI CCNGG 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 2
Bpu10I CCTNAGC 1 cut(s) 129
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 85, 123, 145
BsaWI WCCGGW 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 14
BseAI TCCGGA 1 cut(s) 22
BseDI CCNNGG 3 cut(s) 85, 123, 145
BseGI GGATG 1 cut(s) 159
BseNI ACTGG 1 cut(s) 14
BseXI GCAGC 1 cut(s) 42
BshFI GGCC 1 cut(s) 122
BsiSI CCGG 2 cut(s) 23, 87
BsnI GGCC 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 22
Bsp143I GATC 2 cut(s) 66, 134
BspACI CCGC 3 cut(s) 90, 163, 168
BspANI GGCC 1 cut(s) 122
BspEI TCCGGA 1 cut(s) 22
BspPI GGATC 1 cut(s) 142
BspTI CTTAAG 1 cut(s) 197
BsrBI CCGCTC 1 cut(s) 165
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 3 cut(s) 85, 123, 145
BssMI GATC 2 cut(s) 66, 134
BssT1I CCWWGG 2 cut(s) 123, 145
BstAFI CTTAAG 1 cut(s) 197
BstDEI CTNAG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 159
BstKTI GATC 2 cut(s) 69, 137
BstMBI GATC 2 cut(s) 66, 134
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 2
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuI GTATCC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 122
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 1 cut(s) 159
CviJI RGCY 6 cut(s) 20, 33, 77, 122, 128, 150
CviKI_1 RGCY 6 cut(s) 20, 33, 77, 122, 128, 150
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 68, 136
DpnII GATC 2 cut(s) 66, 134
EciI GGCGGA 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 123, 145
Eco147I AGGCCT 1 cut(s) 122
EcoT14I CCWWGG 2 cut(s) 123, 145
ErhI CCWWGG 2 cut(s) 123, 145
FaiI YATR 2 cut(s) 57, 183
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 31
GluI GCNGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 122
HapII CCGG 2 cut(s) 23, 87
HinfI GANTC 1 cut(s) 156
HpaII CCGG 2 cut(s) 23, 87
Hpy188I TCNGA 1 cut(s) 155
Hpy188III TCNNGA 2 cut(s) 23, 138
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 1 cut(s) 63
Kpn2I TCCGGA 1 cut(s) 22
Kzo9I GATC 2 cut(s) 66, 134
LmnI GCTCC 2 cut(s) 25, 170
LpnPI CCDG 4 cut(s) 27, 36, 100, 123
Lsp1109I GCAGC 1 cut(s) 42
MaeII ACGT 1 cut(s) 63
MalI GATC 2 cut(s) 68, 136
MbiI CCGCTC 1 cut(s) 165
MboI GATC 2 cut(s) 66, 134
MflI RGATCY 1 cut(s) 134
MluCI AATT 1 cut(s) 205
MlyI GAGTC 1 cut(s) 165
MnlI CCTC 2 cut(s) 93, 112
MroI TCCGGA 1 cut(s) 22
MseI TTAA 1 cut(s) 198
MspCI CTTAAG 1 cut(s) 197
MspI CCGG 2 cut(s) 23, 87
MspR9I CCNGG 1 cut(s) 87
NciI CCSGG 1 cut(s) 87
NdeII GATC 2 cut(s) 66, 134
PceI AGGCCT 1 cut(s) 122
PkrI GCNGC 1 cut(s) 32
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PsuI RGATCY 1 cut(s) 134
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 2 cut(s) 66, 134
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 1 cut(s) 87
SetI ASST 4 cut(s) 22, 66, 79, 182
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 1 cut(s) 205
SseBI AGGCCT 1 cut(s) 122
SsiI CCGC 3 cut(s) 90, 163, 168
StuI AGGCCT 1 cut(s) 122
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 123, 145
TaiI ACGT 1 cut(s) 66
TaqI TCGA 1 cut(s) 60
TasI AATT 1 cut(s) 205
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseI GCWGC 1 cut(s) 30
TspGWI ACGGA 1 cut(s) 119
Vha464I CTTAAG 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.