pycom01g23720
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003088
Physical Location & Seq
Reverse (-)
20965818 .. 20968360
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23720.3

Sequence Viewer

Length: 1098 bp
ATGGGTTCGACCCCAGAGACTCAGATGACTCCAACCCAAGTCTCCGACGAAGAAGCCAACCTCTTCGCCATGCAGCTAGCCAGCGGCTCCATTCTCCCCATGGTGCTCAAGGCGGCCATCGAGCTCGACCTCCTCGAGGTCATGGCAAAAGCCGGGCCTGGCGCTTTTGTTTCTCCGGCTGACATAGCTTCGCAGCTGCCGACCAAGAACCCCGACGCCCCCGTCATGCTGGACCGCATACTGCGCCTCCTGGCCAGCTACTCCATCCTCACTTACTCCCTCCGCACACTTCCCGACGGCAAAGTCCAGCGGCTGTACGGCCTCGGCCCCGTCTGCAAGTTCTTGACAAAGAACGAGGATGGTGCTTCCATTGGTTCTCTGTGCCTCATGAATCACGACAAGGTCCTTATGGAGAGCTGGTACCACTTGAAAGAGTCAGTCCTTGAAGGAGGTATCTCATTCAACAAGGCCTATGGAATGACCGCTTTTGAGTACCATGGCACTGACCCAAGATTCAACAAGGTCTTCAACAAGGGAATGGCTGACCACTCTACCATTACCATGAAGAAACTTCTTGAGATCTATAATGGCTTTGAGGGCCTCACATCCATCATTGACGTTGGTGGTGGCACCGGCGCTGTCCTTAACATGATCGTCTCTAAATACCCTTCGATTAAGGGCATTAACTTCGACTTGCCTCATGTCATTGAAGATGCTCCCCAATATCCTGGTGTGGAGCATGTTGGAGGAGACATGTTTGTAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGTGGATATGCCATGACTGGAGTGACGAGCATTGCTTGAAATTTTTGAAGAACTGCTATGCTGCGCTCCCAGACAATGGGAAGGTAATTGTTGCTGAGTGCATTCTTCCGGTGGCTCCGGACAGTAGCCTTGCCACCAAGGTAGTTGTCCATATCGACGCGATCATGTTAGCTCACAACCCCGGCGGAAAAGAGAGGACGGAGAAGGAGTTTGAGGCCTTGGCTAAGGGATCTGGATTCCAAGGCTTTCGGGTCGTGTGTTGTGCTTTCAACACCTATGCCATTGAATTTCTTAAGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

366

Amino Acids

39.79

Weight (kDa)

5.71

Isoelectric Point (pI)

31.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 221, 302
AbsI CCTCGAGG 1 cut(s) 134
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 2 cut(s) 420, 629
AccB7I CCANNNNNTGG 1 cut(s) 869
AccII CGCG 1 cut(s) 953
AccIII TCCGGA 1 cut(s) 910
AciI CCGC 7 cut(s) 84, 113, 235, 283, 310, 483, 978
AclWI GGATC 1 cut(s) 1030
AcoI YGGCCR 2 cut(s) 114, 252
AcsI RAATTY 2 cut(s) 833, 1079
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 3 cut(s) 317, 422, 494
AfiI CCNNNNNNNGG 2 cut(s) 136, 869
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 1 cut(s) 753
AjnI CCWGG 3 cut(s) 157, 249, 727
AluBI AGCT 7 cut(s) 76, 124, 188, 196, 258, 417, 965
AluI AGCT 7 cut(s) 76, 124, 188, 196, 258, 417, 965
Alw21I GWGCWC 2 cut(s) 108, 126
Alw26I GTCTC 4 cut(s) 11, 46, 661, 744
AlwI GGATC 1 cut(s) 1030
AlwNI CAGNNNCTG 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 8 cut(s) 114, 155, 252, 319, 325, 468, 598, 1008
ApeKI GCWGC 4 cut(s) 73, 193, 196, 854
ApoI RAATTY 2 cut(s) 833, 1079
Asp718I GGTACC 1 cut(s) 420
AspLEI GCGC 4 cut(s) 164, 246, 638, 859
AspS9I GGNCC 5 cut(s) 155, 232, 326, 403, 598
AsuC2I CCSGG 2 cut(s) 154, 975
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 2 cut(s) 232, 403
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 2 cut(s) 420, 629
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 2 cut(s) 108, 126
BbvI GCAGC 4 cut(s) 85, 183, 205, 841
BccI CCATC 4 cut(s) 125, 272, 353, 617
BceAI ACGGC 2 cut(s) 313, 334
BcgI CGANNNNNNTGC 4 cut(s) 344, 378, 670, 704
BciT130I CCWGG 3 cut(s) 159, 251, 729
BcnI CCSGG 2 cut(s) 154, 975
BcoDI GTCTC 4 cut(s) 11, 46, 661, 744
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 2 cut(s) 165, 639
BfrI CTTAAG 1 cut(s) 1085
BglII AGATCT 1 cut(s) 579
BisI GCNGC 7 cut(s) 74, 85, 114, 194, 197, 311, 855
BlsI GCNGC 7 cut(s) 75, 86, 115, 195, 198, 312, 856
Bme1390I CCNGG 5 cut(s) 154, 159, 251, 729, 975
Bme18I GGWCC 2 cut(s) 232, 403
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 5 cut(s) 155, 232, 326, 403, 598
BmiI GGNNCC 5 cut(s) 88, 328, 422, 631, 909
BmrFI CCNGG 5 cut(s) 154, 159, 251, 729, 975
BmsI GCATC 2 cut(s) 703, 769
BmtI GCTAGC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 832
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 2 cut(s) 92, 596
BpuMI CCSGG 2 cut(s) 154, 975
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 7 cut(s) 99, 322, 496, 930, 973, 1011, 1033
BsaWI WCCGGW 2 cut(s) 901, 910
BsaXI ACNNNNNCTCC 6 cut(s) 231, 261, 404, 434, 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 869
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 823
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 3 cut(s) 159, 251, 729
BseDI CCNNGG 7 cut(s) 99, 322, 496, 930, 973, 1011, 1033
BseGI GGATG 3 cut(s) 264, 364, 605
BseLI CCNNNNNNNGG 2 cut(s) 136, 869
BseMI GCAATG 1 cut(s) 823
BseMII CTCAG 2 cut(s) 35, 879
BseNI ACTGG 1 cut(s) 815
BseRI GAGGAG 2 cut(s) 122, 762
BseXI GCAGC 4 cut(s) 85, 183, 205, 841
Bsh1236I CGCG 1 cut(s) 953
BshFI GGCC 8 cut(s) 116, 157, 254, 321, 327, 470, 600, 1010
BshNI GGYRCC 2 cut(s) 420, 629
BsiHKAI GWGCWC 2 cut(s) 108, 126
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 6 cut(s) 153, 176, 633, 902, 911, 975
BslI CCNNNNNNNGG 2 cut(s) 136, 869
BsmAI GTCTC 4 cut(s) 11, 46, 661, 744
BsmBI CGTCTC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 8 cut(s) 116, 157, 254, 321, 327, 470, 600, 1010
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 108, 126
Bsp13I TCCGGA 1 cut(s) 910
Bsp143I GATC 4 cut(s) 579, 651, 954, 1022
Bsp19I CCATGG 2 cut(s) 99, 496
BspACI CCGC 7 cut(s) 84, 113, 235, 283, 310, 483, 978
BspANI GGCC 8 cut(s) 116, 157, 254, 321, 327, 470, 600, 1010
BspCNI CTCAG 2 cut(s) 34, 880
BspEI TCCGGA 1 cut(s) 910
BspFNI CGCG 1 cut(s) 953
BspHI TCATGA 2 cut(s) 387, 789
BspLI GGNNCC 5 cut(s) 88, 328, 422, 631, 909
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 1 cut(s) 1030
BspT107I GGYRCC 2 cut(s) 420, 629
BspTI CTTAAG 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 823
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 1 cut(s) 815
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 7 cut(s) 99, 322, 496, 930, 973, 1011, 1033
BssMI GATC 4 cut(s) 579, 651, 954, 1022
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 5 cut(s) 99, 496, 930, 1011, 1033
Bst2UI CCWGG 3 cut(s) 159, 251, 729
Bst4CI ACNGT 1 cut(s) 917
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 1 cut(s) 1085
BstC8I GCNNGC 3 cut(s) 78, 82, 256
BstDEI CTNAG 3 cut(s) 21, 888, 1017
BstDSI CCRYGG 2 cut(s) 99, 496
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 3 cut(s) 264, 364, 605
BstFNI CGCG 1 cut(s) 953
BstH2I RGCGCY 2 cut(s) 165, 639
BstHHI GCGC 4 cut(s) 164, 246, 638, 859
BstKTI GATC 4 cut(s) 582, 654, 957, 1025
BstMAI GTCTC 4 cut(s) 11, 46, 661, 744
BstMBI GATC 4 cut(s) 579, 651, 954, 1022
BstMWI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
BstNI CCWGG 3 cut(s) 159, 251, 729
BstNSI RCATGY 2 cut(s) 743, 757
BstSCI CCNGG 5 cut(s) 152, 157, 249, 727, 973
BstUI CGCG 1 cut(s) 953
BstV1I GCAGC 4 cut(s) 85, 183, 205, 841
BstV2I GAAGAC 1 cut(s) 517
BstX2I RGATCY 2 cut(s) 579, 1022
BstXI CCANNNNNNTGG 1 cut(s) 728
BstYI RGATCY 2 cut(s) 579, 1022
BsuRI GGCC 8 cut(s) 116, 157, 254, 321, 327, 470, 600, 1010
BtgI CCRYGG 2 cut(s) 99, 496
BtsCI GGATG 3 cut(s) 264, 364, 605
BtsIMutI CAGTG 1 cut(s) 501
Cac8I GCNNGC 3 cut(s) 78, 82, 256
CaiI CAGNNNCTG 1 cut(s) 313
CciI TCATGA 2 cut(s) 387, 789
CfoI GCGC 4 cut(s) 164, 246, 638, 859
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 5 cut(s) 155, 232, 326, 403, 598
CseI GACGC 2 cut(s) 224, 959
Csp6I GTAC 3 cut(s) 316, 421, 493
CviQI GTAC 3 cut(s) 316, 421, 493
DdeI CTNAG 3 cut(s) 21, 888, 1017
DpnI GATC 4 cut(s) 581, 653, 956, 1024
DpnII GATC 4 cut(s) 579, 651, 954, 1022
DrdI GACNNNNNNGTC 2 cut(s) 221, 302
DseDI GACNNNNNNGTC 2 cut(s) 221, 302
EaeI YGGCCR 2 cut(s) 114, 252
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 993
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 5 cut(s) 99, 496, 930, 1011, 1033
Eco147I AGGCCT 2 cut(s) 470, 1010
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 2 cut(s) 232, 403
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 2 cut(s) 403, 598
EcoRII CCWGG 3 cut(s) 157, 249, 727
EcoT14I CCWWGG 5 cut(s) 99, 496, 930, 1011, 1033
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 5 cut(s) 99, 496, 930, 1011, 1033
Esp3I CGTCTC 1 cut(s) 661
Fnu4HI GCNGC 7 cut(s) 74, 85, 114, 194, 197, 311, 855
FokI GGATG 3 cut(s) 251, 371, 592
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 7 cut(s) 74, 85, 114, 194, 197, 311, 855
FspBI CTAG 1 cut(s) 77
GlaI GCGC 4 cut(s) 163, 245, 637, 858
GluI GCNGC 7 cut(s) 74, 85, 114, 194, 197, 311, 855
GsuI CTGGAG 1 cut(s) 832
HaeII RGCGCY 2 cut(s) 165, 639
HaeIII GGCC 8 cut(s) 116, 157, 254, 321, 327, 470, 600, 1010
HapII CCGG 6 cut(s) 153, 176, 633, 902, 911, 975
HgaI GACGC 2 cut(s) 224, 959
HhaI GCGC 4 cut(s) 164, 246, 638, 859
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 4 cut(s) 162, 244, 636, 857
HinP1I GCGC 4 cut(s) 162, 244, 636, 857
HinfI GANTC 6 cut(s) 19, 28, 391, 434, 513, 1029
HpaII CCGG 6 cut(s) 153, 176, 633, 902, 911, 975
Hpy188I TCNGA 2 cut(s) 24, 46
Hpy188III TCNNGA 8 cut(s) 293, 343, 388, 395, 575, 790, 911, 1026
Hpy99I CGWCG 4 cut(s) 50, 218, 299, 953
HpyAV CCTTC 4 cut(s) 440, 678, 868, 991
HpyCH4III ACNGT 1 cut(s) 917
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 4 cut(s) 73, 336, 782, 894
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
HpyF3I CTNAG 3 cut(s) 21, 888, 1017
HpySE526I ACGT 1 cut(s) 618
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 4 cut(s) 162, 244, 636, 857
Kpn2I TCCGGA 1 cut(s) 910
KpnI GGTACC 1 cut(s) 424
Kzo9I GATC 4 cut(s) 579, 651, 954, 1022
LmnI GCTCC 5 cut(s) 92, 721, 736, 864, 913
Lsp1109I GCAGC 4 cut(s) 85, 183, 205, 841
LweI GCATC 2 cut(s) 703, 769
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 1 cut(s) 815
MalI GATC 4 cut(s) 581, 653, 956, 1024
MboI GATC 4 cut(s) 579, 651, 954, 1022
MboII GAAGA 7 cut(s) 55, 62, 517, 577, 722, 853, 890
MflI RGATCY 2 cut(s) 579, 1022
MhlI GDGCHC 2 cut(s) 108, 126
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 5 cut(s) 783, 833, 879, 1079, 1093
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 3 cut(s) 13, 22, 443
MmeI TCCRAC 3 cut(s) 56, 69, 724
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 910
MscI TGGCCA 1 cut(s) 254
MseI TTAA 4 cut(s) 645, 675, 684, 1086
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 3 cut(s) 84, 196, 310
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 6 cut(s) 153, 176, 633, 902, 911, 975
MspR9I CCNGG 5 cut(s) 154, 159, 251, 729, 975
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 3 cut(s) 159, 251, 729
MvnI CGCG 1 cut(s) 953
MwoI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
NciI CCSGG 2 cut(s) 154, 975
NcoI CCATGG 2 cut(s) 99, 496
NdeII GATC 4 cut(s) 579, 651, 954, 1022
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 5 cut(s) 88, 328, 422, 631, 909
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 815
NspI RCATGY 2 cut(s) 743, 757
PaeR7I CTCGAG 1 cut(s) 134
PagI TCATGA 2 cut(s) 387, 789
PceI AGGCCT 2 cut(s) 470, 1010
PciI ACATGT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 197
PctI GAATGC 1 cut(s) 894
PfeI GAWTC 3 cut(s) 391, 513, 1029
PflFI GACNNNGTC 1 cut(s) 401
PflMI CCANNNNNTGG 1 cut(s) 869
PkrI GCNGC 7 cut(s) 75, 86, 115, 195, 198, 312, 856
PleI GAGTC 3 cut(s) 13, 22, 442
PpsI GAGTC 3 cut(s) 13, 22, 442
PpuMI RGGWCCY 1 cut(s) 403
PscI ACATGT 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 1 cut(s) 403
Psp6I CCWGG 3 cut(s) 157, 249, 727
PspGI CCWGG 3 cut(s) 157, 249, 727
PspN4I GGNNCC 5 cut(s) 88, 328, 422, 631, 909
PspPI GGNCC 5 cut(s) 155, 232, 326, 403, 598
PspPPI RGGWCCY 1 cut(s) 403
PspXI VCTCGAGB 1 cut(s) 134
PstNI CAGNNNCTG 1 cut(s) 313
PsuI RGATCY 2 cut(s) 579, 1022
PsyI GACNNNGTC 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 3 cut(s) 317, 422, 494
RsaNI GTAC 3 cut(s) 316, 421, 493
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 4 cut(s) 645, 675, 684, 1086
SatI GCNGC 7 cut(s) 74, 85, 114, 194, 197, 311, 855
Sau3AI GATC 4 cut(s) 579, 651, 954, 1022
Sau96I GGNCC 5 cut(s) 155, 232, 326, 403, 598
SchI GAGTC 3 cut(s) 13, 22, 443
ScrFI CCNGG 5 cut(s) 154, 159, 251, 729, 975
SduI GDGCHC 2 cut(s) 108, 126
SfaNI GCATC 2 cut(s) 703, 769
Sfr274I CTCGAG 1 cut(s) 134
SgrAI CRCCGGYG 1 cut(s) 632
SinI GGWCC 2 cut(s) 232, 403
SlaI CTCGAG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 4 cut(s) 107, 134, 575, 1085
SmoI CTYRAG 4 cut(s) 107, 134, 575, 1085
Sse9I AATT 5 cut(s) 783, 833, 879, 1079, 1093
SseBI AGGCCT 2 cut(s) 470, 1010
SsiI CCGC 7 cut(s) 84, 113, 235, 283, 310, 483, 978
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 2 cut(s) 470, 1010
StyD4I CCNGG 5 cut(s) 152, 157, 249, 727, 973
StyI CCWWGG 5 cut(s) 99, 496, 930, 1011, 1033
TaaI ACNGT 1 cut(s) 917
TaiI ACGT 1 cut(s) 621
TaqI TCGA 7 cut(s) 8, 120, 126, 135, 671, 690, 948
TasI AATT 5 cut(s) 783, 833, 879, 1079, 1093
TauI GCSGC 3 cut(s) 87, 116, 313
TfiI GAWTC 3 cut(s) 391, 513, 1029
Tru1I TTAA 4 cut(s) 645, 675, 684, 1086
Tru9I TTAA 4 cut(s) 645, 675, 684, 1086
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 1 cut(s) 815
TseI GCWGC 4 cut(s) 73, 193, 196, 854
Tsp45I GTSAC 1 cut(s) 815
TspDTI ATGAA 4 cut(s) 404, 578, 778, 806
TspGWI ACGGA 1 cut(s) 1007
TspRI CASTG 1 cut(s) 508
Tth111I GACNNNGTC 1 cut(s) 401
Van91I CCANNNNNTGG 1 cut(s) 869
Vha464I CTTAAG 1 cut(s) 1085
VpaK11BI GGWCC 2 cut(s) 232, 403
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 2 cut(s) 833, 1079
XceI RCATGY 2 cut(s) 743, 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XhoI CTCGAG 1 cut(s) 134
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.