pycom07g27330
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003088
Physical Location & Seq
Reverse (-)
27097454 .. 27102023
4570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27330.4

Sequence Viewer

Length: 1020 bp
ATGGGTTCGACCGTAGAGACTCAGATGACTCCAACCCAAGTCTCCAACGAAGACGAAGAAGCAAACCTCTTCGCCATGCAGCTAGCCAGCGCCTCTGTTCTCCCCATGGTGCTCAATGCAGCCCTTGAGCTCGACCTCCTCGAGATCATGGCGAGAGCAGGGCCTGGCGCTTTCGTTTCTCCGGCGGACTTAGCTTCGCAGCTGCCGACCAAGAACCCTGACGCCACCGTCATGCTGGACCGCATGCTGCGCCTCCTGGCCAGCTACTCCATCCTCACTTGCTCCCTCCGCACGCTTCGCGACGGCAATGTCGAGCGGCTGTACGGCCTCGGCCCCGTCTGCAAGTTCTTGATAAAGAACGAGGATGGTGTTTCCATTGCTCCTCTCTGCCTCATGAATCAGGACAAGGTCCTTATGGAGAGCTGGTACCACTTGAAAGATGCTGTTCTTGAAGGAGGAATTCCATTCAACAAGGCCTATGGAATGACTGCTTTTGAGTACCATGGCACTGACCCTAGATTCAACAAGGTCTTCAACGCAGGAATGGCTGCCCACTCTACCATTACCATGAAAAAACTTCTAGAGACCTACAAGGGCTTTCAGGGCCTCACATCCGTAGTAGATGTTGGTGGTGGCACTGGCGCTGTTATTAACATGATCGTTTCTAAGTACCCCTCGATTAAGGGTATTAACTTCGATCTGCCTCATGTCATAGAAGATGCTCCCCAATATCCTGGTGTGGAGCATGTTGGAGGAGACATGTTTGTAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGTTTATATGTCACGACTGGAGTGACGATCATTGCTTGAAATTTTTGAAGAACTGTTATACCGCGCTCCCTGACAATGGGAAGGTGATTCTTGCTGAGTGCATTCTTCCAGTAGCTCCAGCCAGTAGCCTTGCCACCAAGGGTGTTGTCCATATCGACGACTTGATTGATCATGTTAGCTCACAACCCCGGAGGAAAAGAGCGGACGGAGAAGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

340

Amino Acids

36.8

Weight (kDa)

5.37

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 227, 308
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 1 cut(s) 426
AccBSI CCGCTC 2 cut(s) 316, 1001
AccII CGCG 2 cut(s) 300, 863
AciI CCGC 6 cut(s) 185, 241, 289, 316, 861, 1001
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 459, 839
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 4 cut(s) 323, 428, 500, 671
AfiI CCNNNNNNNGG 1 cut(s) 875
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 7 cut(s) 436, 452, 469, 523, 535, 838, 847
AjnI CCWGG 3 cut(s) 163, 255, 733
AluBI AGCT 8 cut(s) 82, 130, 194, 202, 264, 423, 914, 978
AluI AGCT 8 cut(s) 82, 130, 194, 202, 264, 423, 914, 978
Alw21I GWGCWC 2 cut(s) 114, 132
Alw26I GTCTC 4 cut(s) 11, 46, 578, 750
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 6 cut(s) 161, 258, 325, 331, 474, 604
ApeKI GCWGC 6 cut(s) 79, 119, 199, 202, 247, 548
ApoI RAATTY 2 cut(s) 459, 839
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 5 cut(s) 92, 170, 252, 644, 865
AspS9I GGNCC 5 cut(s) 161, 238, 332, 409, 604
AsuC2I CCSGG 1 cut(s) 988
AsuHPI GGTGA 1 cut(s) 895
AsuNHI GCTAGC 1 cut(s) 82
AvaI CYCGRG 1 cut(s) 140
AvaII GGWCC 2 cut(s) 238, 409
BalI TGGCCA 1 cut(s) 260
BanI GGYRCC 1 cut(s) 426
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 2 cut(s) 57, 523
Bbv12I GWGCWC 2 cut(s) 114, 132
BbvI GCAGC 6 cut(s) 91, 131, 189, 211, 234, 535
BccI CCATC 2 cut(s) 278, 359
BceAI ACGGC 2 cut(s) 319, 340
BciT130I CCWGG 3 cut(s) 165, 257, 735
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 1 cut(s) 988
BcoDI GTCTC 4 cut(s) 11, 46, 578, 750
BfaI CTAG 3 cut(s) 83, 516, 581
BfoI RGCGCY 3 cut(s) 93, 171, 645
BisI GCNGC 7 cut(s) 80, 120, 200, 203, 248, 317, 549
BlsI GCNGC 7 cut(s) 81, 121, 201, 204, 249, 318, 550
Bme1390I CCNGG 4 cut(s) 165, 257, 735, 988
Bme18I GGWCC 2 cut(s) 238, 409
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 5 cut(s) 161, 238, 332, 409, 604
BmiI GGNNCC 2 cut(s) 334, 428
BmrFI CCNGG 4 cut(s) 165, 257, 735, 988
BmsI GCATC 3 cut(s) 430, 709, 775
BmtI GCTAGC 1 cut(s) 86
BpiI GAAGAC 2 cut(s) 57, 523
BpmI CTGGAG 2 cut(s) 838, 900
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 988
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 578
BsaJI CCNNGG 5 cut(s) 105, 328, 502, 936, 986
BsaXI ACNNNNNCTCC 3 cut(s) 410, 440, 999
Bsc4I CCNNNNNNNGG 1 cut(s) 875
Bse1I ACTGG 4 cut(s) 643, 821, 908, 921
Bse3DI GCAATG 3 cut(s) 313, 375, 829
BseBI CCWGG 3 cut(s) 165, 257, 735
BseDI CCNNGG 5 cut(s) 105, 328, 502, 936, 986
BseGI GGATG 3 cut(s) 270, 370, 611
BseLI CCNNNNNNNGG 1 cut(s) 875
BseMI GCAATG 3 cut(s) 313, 375, 829
BseMII CTCAG 2 cut(s) 35, 885
BseNI ACTGG 4 cut(s) 643, 821, 908, 921
BseRI GAGGAG 3 cut(s) 128, 372, 768
BseXI GCAGC 6 cut(s) 91, 131, 189, 211, 234, 535
Bsh1236I CGCG 2 cut(s) 300, 863
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 6 cut(s) 163, 260, 327, 333, 476, 606
BshNI GGYRCC 1 cut(s) 426
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 114, 132
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 2 cut(s) 182, 988
BslI CCNNNNNNNGG 1 cut(s) 875
BsmAI GTCTC 4 cut(s) 11, 46, 578, 750
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 6 cut(s) 163, 260, 327, 333, 476, 606
Bso31I GGTCTC 1 cut(s) 578
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 114, 132
Bsp143I GATC 5 cut(s) 144, 657, 697, 826, 967
Bsp19I CCATGG 2 cut(s) 105, 502
Bsp68I TCGCGA 1 cut(s) 300
BspACI CCGC 6 cut(s) 185, 241, 289, 316, 861, 1001
BspANI GGCC 6 cut(s) 163, 260, 327, 333, 476, 606
BspCNI CTCAG 2 cut(s) 34, 886
BspFNI CGCG 2 cut(s) 300, 863
BspHI TCATGA 2 cut(s) 393, 795
BspLI GGNNCC 2 cut(s) 334, 428
BspOI GCTAGC 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 578
BsrBI CCGCTC 2 cut(s) 316, 1001
BsrDI GCAATG 3 cut(s) 313, 375, 829
BsrI ACTGG 4 cut(s) 643, 821, 908, 921
BssECI CCNNGG 5 cut(s) 105, 328, 502, 936, 986
BssMI GATC 5 cut(s) 144, 657, 697, 826, 967
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 3 cut(s) 105, 502, 936
Bst2UI CCWGG 3 cut(s) 165, 257, 735
Bst4CI ACNGT 3 cut(s) 13, 229, 854
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 5 cut(s) 84, 88, 245, 262, 293
BstDEI CTNAG 4 cut(s) 21, 190, 666, 894
BstDSI CCRYGG 2 cut(s) 105, 502
BstF5I GGATG 3 cut(s) 270, 370, 611
BstFNI CGCG 2 cut(s) 300, 863
BstH2I RGCGCY 3 cut(s) 93, 171, 645
BstHHI GCGC 5 cut(s) 92, 170, 252, 644, 865
BstKTI GATC 5 cut(s) 147, 660, 700, 829, 970
BstMAI GTCTC 4 cut(s) 11, 46, 578, 750
BstMBI GATC 5 cut(s) 144, 657, 697, 826, 967
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 7 cut(s) 191, 249, 288, 297, 339, 545, 603
BstNI CCWGG 3 cut(s) 165, 257, 735
BstNSI RCATGY 3 cut(s) 247, 749, 763
BstSCI CCNGG 4 cut(s) 163, 255, 733, 986
BstUI CGCG 2 cut(s) 300, 863
BstV1I GCAGC 6 cut(s) 91, 131, 189, 211, 234, 535
BstV2I GAAGAC 2 cut(s) 57, 523
BstXI CCANNNNNNTGG 1 cut(s) 734
BsuRI GGCC 6 cut(s) 163, 260, 327, 333, 476, 606
BtgI CCRYGG 2 cut(s) 105, 502
BtsCI GGATG 3 cut(s) 270, 370, 611
BtsIMutI CAGTG 2 cut(s) 507, 636
BtuMI TCGCGA 1 cut(s) 300
Cac8I GCNNGC 5 cut(s) 84, 88, 245, 262, 293
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 2 cut(s) 393, 795
CfoI GCGC 5 cut(s) 92, 170, 252, 644, 865
Cfr13I GGNCC 5 cut(s) 161, 238, 332, 409, 604
CseI GACGC 1 cut(s) 230
Csp6I GTAC 4 cut(s) 322, 427, 499, 670
CviQI GTAC 4 cut(s) 322, 427, 499, 670
DdeI CTNAG 4 cut(s) 21, 190, 666, 894
DpnI GATC 5 cut(s) 146, 659, 699, 828, 969
DpnII GATC 5 cut(s) 144, 657, 697, 826, 967
DrdI GACNNNNNNGTC 2 cut(s) 227, 308
DseDI GACNNNNNNGTC 2 cut(s) 227, 308
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 3 cut(s) 105, 502, 936
Eco147I AGGCCT 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 578
Eco47I GGWCC 2 cut(s) 238, 409
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 3 cut(s) 161, 409, 604
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 3 cut(s) 163, 255, 733
EcoT14I CCWWGG 3 cut(s) 105, 502, 936
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 105, 502, 936
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 7 cut(s) 80, 120, 200, 203, 248, 317, 549
FokI GGATG 3 cut(s) 257, 377, 598
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 7 cut(s) 80, 120, 200, 203, 248, 317, 549
FspBI CTAG 3 cut(s) 83, 516, 581
GlaI GCGC 5 cut(s) 91, 169, 251, 643, 864
GluI GCNGC 7 cut(s) 80, 120, 200, 203, 248, 317, 549
GsuI CTGGAG 2 cut(s) 838, 900
HaeII RGCGCY 3 cut(s) 93, 171, 645
HaeIII GGCC 6 cut(s) 163, 260, 327, 333, 476, 606
HapII CCGG 2 cut(s) 182, 988
HgaI GACGC 1 cut(s) 230
HhaI GCGC 5 cut(s) 92, 170, 252, 644, 865
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 5 cut(s) 90, 168, 250, 642, 863
HinP1I GCGC 5 cut(s) 90, 168, 250, 642, 863
HinfI GANTC 5 cut(s) 19, 28, 397, 519, 886
HpaII CCGG 2 cut(s) 182, 988
HphI GGTGA 1 cut(s) 895
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 9 cut(s) 142, 299, 349, 394, 401, 449, 581, 796, 812
Hpy99I CGWCG 2 cut(s) 305, 959
HpyAV CCTTC 3 cut(s) 446, 874, 1004
HpyCH4III ACNGT 3 cut(s) 13, 229, 854
HpyCH4V TGCA 5 cut(s) 79, 119, 342, 788, 900
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 249, 288, 297, 339, 545, 603
HpyF3I CTNAG 4 cut(s) 21, 190, 666, 894
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 5 cut(s) 90, 168, 250, 642, 863
KpnI GGTACC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 5 cut(s) 144, 657, 697, 826, 967
LmnI GCTCC 6 cut(s) 287, 385, 727, 742, 870, 919
Lsp1109I GCAGC 6 cut(s) 91, 131, 189, 211, 234, 535
LweI GCATC 3 cut(s) 430, 709, 775
MaeI CTAG 3 cut(s) 83, 516, 581
MaeIII GTNAC 2 cut(s) 809, 821
MalI GATC 5 cut(s) 146, 659, 699, 828, 969
MbiI CCGCTC 2 cut(s) 316, 1001
MboI GATC 5 cut(s) 144, 657, 697, 826, 967
MboII GAAGA 7 cut(s) 61, 62, 68, 523, 728, 859, 896
MhlI GDGCHC 2 cut(s) 114, 132
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 3 cut(s) 459, 789, 839
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 2 cut(s) 13, 22
MmeI TCCRAC 3 cut(s) 56, 69, 730
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
MseI TTAA 3 cut(s) 651, 681, 690
MslI CAYNNNNRTG 2 cut(s) 230, 566
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 2 cut(s) 182, 988
MspR9I CCNGG 4 cut(s) 165, 257, 735, 988
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 3 cut(s) 165, 257, 735
MvnI CGCG 2 cut(s) 300, 863
MwoI GCNNNNNNNGC 7 cut(s) 191, 249, 288, 297, 339, 545, 603
NciI CCSGG 1 cut(s) 988
NcoI CCATGG 2 cut(s) 105, 502
NdeII GATC 5 cut(s) 144, 657, 697, 826, 967
NheI GCTAGC 1 cut(s) 82
NlaIV GGNNCC 2 cut(s) 334, 428
NmeAIII GCCGAG 1 cut(s) 309
NmuCI GTSAC 2 cut(s) 809, 821
NruI TCGCGA 1 cut(s) 300
NspI RCATGY 3 cut(s) 247, 749, 763
PaeI GCATGC 1 cut(s) 247
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 2 cut(s) 393, 795
PceI AGGCCT 1 cut(s) 476
PciI ACATGT 1 cut(s) 759
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 203, 309
PctI GAATGC 1 cut(s) 900
PfeI GAWTC 3 cut(s) 397, 519, 886
PflFI GACNNNGTC 1 cut(s) 407
PkrI GCNGC 7 cut(s) 81, 121, 201, 204, 249, 318, 550
PleI GAGTC 2 cut(s) 13, 22
PpsI GAGTC 2 cut(s) 13, 22
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 3 cut(s) 163, 255, 733
PspGI CCWGG 3 cut(s) 163, 255, 733
PspN4I GGNNCC 2 cut(s) 334, 428
PspPI GGNCC 5 cut(s) 161, 238, 332, 409, 604
PspPPI RGGWCCY 1 cut(s) 409
PstNI CAGNNNCTG 1 cut(s) 164
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 202
RruI TCGCGA 1 cut(s) 300
RsaI GTAC 4 cut(s) 323, 428, 500, 671
RsaNI GTAC 4 cut(s) 322, 427, 499, 670
RseI CAYNNNNRTG 2 cut(s) 230, 566
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 651, 681, 690
SatI GCNGC 7 cut(s) 80, 120, 200, 203, 248, 317, 549
Sau3AI GATC 5 cut(s) 144, 657, 697, 826, 967
Sau96I GGNCC 5 cut(s) 161, 238, 332, 409, 604
SchI GAGTC 2 cut(s) 13, 22
ScrFI CCNGG 4 cut(s) 165, 257, 735, 988
SduI GDGCHC 2 cut(s) 114, 132
SfaNI GCATC 3 cut(s) 430, 709, 775
Sfr274I CTCGAG 1 cut(s) 140
SinI GGWCC 2 cut(s) 238, 409
SlaI CTCGAG 1 cut(s) 140
SmiMI CAYNNNNRTG 2 cut(s) 230, 566
SmlI CTYRAG 2 cut(s) 125, 140
SmoI CTYRAG 2 cut(s) 125, 140
SphI GCATGC 1 cut(s) 247
Sse9I AATT 3 cut(s) 459, 789, 839
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 6 cut(s) 185, 241, 289, 316, 861, 1001
SspMI CTAG 3 cut(s) 83, 516, 581
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 4 cut(s) 163, 255, 733, 986
StyI CCWWGG 3 cut(s) 105, 502, 936
TaaI ACNGT 3 cut(s) 13, 229, 854
TaqI TCGA 7 cut(s) 8, 132, 141, 312, 677, 696, 954
TasI AATT 3 cut(s) 459, 789, 839
TauI GCSGC 1 cut(s) 319
TfiI GAWTC 3 cut(s) 397, 519, 886
Tru1I TTAA 3 cut(s) 651, 681, 690
Tru9I TTAA 3 cut(s) 651, 681, 690
TscAI CASTG 2 cut(s) 514, 643
TseFI GTSAC 2 cut(s) 809, 821
TseI GCWGC 6 cut(s) 79, 119, 199, 202, 247, 548
Tsp45I GTSAC 2 cut(s) 809, 821
TspDTI ATGAA 4 cut(s) 410, 584, 784, 812
TspGWI ACGGA 2 cut(s) 604, 1020
TspRI CASTG 2 cut(s) 514, 643
Tth111I GACNNNGTC 1 cut(s) 407
VpaK11BI GGWCC 2 cut(s) 238, 409
XapI RAATTY 2 cut(s) 459, 839
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 3 cut(s) 247, 749, 763
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 140
XspI CTAG 3 cut(s) 83, 516, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.