pycom07g27240
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003088
Physical Location & Seq
Reverse (-)
27008611 .. 27012250
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27240.1

Sequence Viewer

Length: 822 bp
ATGGGTTCGACCGGAGAGACTCAGATGACTCCAACCCAAGTCTCCGACGAAGAAACCAACCTCTTCGCCATGCAGCTAGCCAGCGCCTCCATTCCCCCCATGGTGCTCAAGGCAGCCCTCGAGCTCGACCTCCTCGAGATCATGGCGAAAGCCGGGCCTGGCGCTTTCGTTTCTCCGGCGGACTTAGCTTCGCAGCTGCCAACCAAGAACCCTGACGCCCCTGTCATCCTGGACCGCATGCTGCGCCTCCTGGCCAGCTACTCGATCCTCACTTACTCCCTCCGCAAGCTTCCCGATGGCAAAGTAGAGCGGCTGTACGGCCTCGGCCCCGTCTGCAAGTTATTGACAAAGAATGAGGATGGTGTTTCCATTGCTCCTCTCTTACTCCTGACTCAGGACAAGGTCTTTGTTGAGAGCTGGTACCACTTGAAAGATGCTGTTCTTGAAGGAGGAATTGCATTCAACAAGGCCTATGGAATGACTACTTTTGAGTACCATGGCACTGACCTTAGATTCAACAAGGTTTTTAACAGGGCAATGGCTGACCACTCTAGCATTGCCATGAAGAAACTTCTTGAGACCTACATGGGCTTTGAGGGCCTCACATCCGTCGTTGATGTTGGTGGTGGTATCGGCAGTGTTATTCACATGATTGTCTCTAAGTACCCCTCAATTAAGGGCATTAACTTCGACTTGCCTCATGTCATCAAAGATGCTCCCCAATATCCTGGTGTGGAGCATGTTGGAGGAGATATGTTTGTAAGTGTTCCAAAGGGAGATGCAATTGTCATGAAAGTGACATATATGTCACGATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

274

Amino Acids

29.7

Weight (kDa)

5.71

Isoelectric Point (pI)

27.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 24 - 117 1.1e-15 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 140 - 265 4.3e-43 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 221, 805
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 420
AccBSI CCGCTC 1 cut(s) 310
AciI CCGC 4 cut(s) 179, 235, 283, 310
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 4 cut(s) 317, 422, 494, 665
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 4 cut(s) 430, 446, 463, 517
AjnI CCWGG 4 cut(s) 157, 228, 249, 727
AluBI AGCT 7 cut(s) 76, 124, 188, 196, 258, 289, 417
AluI AGCT 7 cut(s) 76, 124, 188, 196, 258, 289, 417
Alw21I GWGCWC 2 cut(s) 108, 126
Alw26I GTCTC 4 cut(s) 11, 46, 572, 661
AlwI GGATC 1 cut(s) 259
Ama87I CYCGRG 2 cut(s) 119, 134
AoxI GGCC 6 cut(s) 155, 252, 319, 325, 468, 598
ApeKI GCWGC 5 cut(s) 73, 113, 193, 196, 241
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
Asp718I GGTACC 1 cut(s) 420
AspLEI GCGC 3 cut(s) 86, 164, 246
AspS9I GGNCC 4 cut(s) 155, 232, 326, 598
AsuC2I CCSGG 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 119, 134
AvaII GGWCC 1 cut(s) 232
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 108, 126
BbvI GCAGC 5 cut(s) 85, 125, 183, 205, 228
BccI CCATC 2 cut(s) 290, 353
BceAI ACGGC 1 cut(s) 334
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 4 cut(s) 159, 230, 251, 729
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 4 cut(s) 11, 46, 572, 661
BfaI CTAG 2 cut(s) 77, 552
BfoI RGCGCY 2 cut(s) 87, 165
BisI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
BlsI GCNGC 6 cut(s) 75, 115, 195, 198, 243, 312
Bme1390I CCNGG 5 cut(s) 154, 159, 230, 251, 729
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 2 cut(s) 119, 134
BmgT120I GGNCC 4 cut(s) 155, 232, 326, 598
BmiI GGNNCC 2 cut(s) 328, 422
BmrFI CCNGG 5 cut(s) 154, 159, 230, 251, 729
BmsI GCATC 3 cut(s) 424, 703, 769
BmtI GCTAGC 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 92, 596
BpuMI CCSGG 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 99, 322, 496
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse3DI GCAATG 3 cut(s) 369, 543, 555
BseBI CCWGG 4 cut(s) 159, 230, 251, 729
BseDI CCNNGG 3 cut(s) 99, 322, 496
BseGI GGATG 3 cut(s) 225, 364, 605
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 3 cut(s) 369, 543, 555
BseMII CTCAG 2 cut(s) 35, 407
BseRI GAGGAG 3 cut(s) 122, 366, 762
BseXI GCAGC 5 cut(s) 85, 125, 183, 205, 228
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 6 cut(s) 157, 254, 321, 327, 470, 600
BshNI GGYRCC 1 cut(s) 420
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 108, 126
BsiHKCI CYCGRG 2 cut(s) 119, 134
BsiSI CCGG 3 cut(s) 12, 153, 176
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 4 cut(s) 11, 46, 572, 661
BsmI GAATGC 1 cut(s) 458
BsnI GGCC 6 cut(s) 157, 254, 321, 327, 470, 600
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 2 cut(s) 119, 134
Bsp1286I GDGCHC 2 cut(s) 108, 126
Bsp143I GATC 2 cut(s) 138, 264
Bsp19I CCATGG 2 cut(s) 99, 496
BspACI CCGC 4 cut(s) 179, 235, 283, 310
BspANI GGCC 6 cut(s) 157, 254, 321, 327, 470, 600
BspCNI CTCAG 2 cut(s) 34, 406
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 2 cut(s) 328, 422
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 1 cut(s) 259
BspT107I GGYRCC 1 cut(s) 420
BspTNI GGTCTC 1 cut(s) 572
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 3 cut(s) 369, 543, 555
BssECI CCNNGG 3 cut(s) 99, 322, 496
BssMI GATC 2 cut(s) 138, 264
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 2 cut(s) 99, 496
Bst2UI CCWGG 4 cut(s) 159, 230, 251, 729
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 5 cut(s) 78, 82, 239, 256, 287
BstDEI CTNAG 5 cut(s) 21, 184, 393, 509, 660
BstDSI CCRYGG 2 cut(s) 99, 496
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 3 cut(s) 225, 364, 605
BstH2I RGCGCY 2 cut(s) 87, 165
BstHHI GCGC 3 cut(s) 86, 164, 246
BstKTI GATC 2 cut(s) 141, 267
BstMAI GTCTC 4 cut(s) 11, 46, 572, 661
BstMBI GATC 2 cut(s) 138, 264
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
BstNI CCWGG 4 cut(s) 159, 230, 251, 729
BstNSI RCATGY 2 cut(s) 241, 743
BstSCI CCNGG 5 cut(s) 152, 157, 228, 249, 727
BstV1I GCAGC 5 cut(s) 85, 125, 183, 205, 228
BstXI CCANNNNNNTGG 1 cut(s) 728
BsuRI GGCC 6 cut(s) 157, 254, 321, 327, 470, 600
BtgI CCRYGG 2 cut(s) 99, 496
BtsCI GGATG 3 cut(s) 225, 364, 605
BtsI GCAGTG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 501, 643
Cac8I GCNNGC 5 cut(s) 78, 82, 239, 256, 287
CciI TCATGA 1 cut(s) 789
CfoI GCGC 3 cut(s) 86, 164, 246
Cfr13I GGNCC 4 cut(s) 155, 232, 326, 598
CseI GACGC 1 cut(s) 224
Csp6I GTAC 4 cut(s) 316, 421, 493, 664
CviQI GTAC 4 cut(s) 316, 421, 493, 664
DdeI CTNAG 5 cut(s) 21, 184, 393, 509, 660
DpnI GATC 2 cut(s) 140, 266
DpnII GATC 2 cut(s) 138, 264
DrdI GACNNNNNNGTC 2 cut(s) 221, 805
DseDI GACNNNNNNGTC 2 cut(s) 221, 805
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 2 cut(s) 99, 496
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 119, 134
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 4 cut(s) 157, 228, 249, 727
EcoT14I CCWWGG 2 cut(s) 99, 496
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 2 cut(s) 99, 496
Fnu4HI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
FokI GGATG 3 cut(s) 212, 371, 592
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
FspBI CTAG 2 cut(s) 77, 552
GlaI GCGC 3 cut(s) 85, 163, 245
GluI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
HaeII RGCGCY 2 cut(s) 87, 165
HaeIII GGCC 6 cut(s) 157, 254, 321, 327, 470, 600
HapII CCGG 3 cut(s) 12, 153, 176
HgaI GACGC 1 cut(s) 224
HhaI GCGC 3 cut(s) 86, 164, 246
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 3 cut(s) 84, 162, 244
HinP1I GCGC 3 cut(s) 84, 162, 244
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 4 cut(s) 19, 28, 391, 513
HpaII CCGG 3 cut(s) 12, 153, 176
Hpy188I TCNGA 2 cut(s) 24, 46
Hpy188III TCNNGA 8 cut(s) 136, 293, 388, 395, 443, 575, 790, 810
Hpy99I CGWCG 2 cut(s) 50, 614
HpyAV CCTTC 1 cut(s) 440
HpyCH4V TGCA 4 cut(s) 73, 336, 458, 782
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
HpyF3I CTNAG 5 cut(s) 21, 184, 393, 509, 660
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 3 cut(s) 84, 162, 244
KpnI GGTACC 1 cut(s) 424
Kzo9I GATC 2 cut(s) 138, 264
LmnI GCTCC 3 cut(s) 379, 721, 736
Lsp1109I GCAGC 5 cut(s) 85, 125, 183, 205, 228
LweI GCATC 3 cut(s) 424, 703, 769
MaeI CTAG 2 cut(s) 77, 552
MaeIII GTNAC 2 cut(s) 796, 807
MalI GATC 2 cut(s) 140, 266
MbiI CCGCTC 1 cut(s) 310
MboI GATC 2 cut(s) 138, 264
MboII GAAGA 3 cut(s) 55, 62, 577
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 2 cut(s) 108, 126
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 3 cut(s) 453, 672, 783
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 3 cut(s) 13, 22, 385
MmeI TCCRAC 3 cut(s) 56, 69, 724
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 3 cut(s) 528, 675, 684
MslI CAYNNNNRTG 2 cut(s) 560, 794
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 3 cut(s) 12, 153, 176
MspR9I CCNGG 5 cut(s) 154, 159, 230, 251, 729
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 4 cut(s) 159, 230, 251, 729
MwoI GCNNNNNNNGC 4 cut(s) 185, 243, 333, 597
NciI CCSGG 1 cut(s) 154
NcoI CCATGG 2 cut(s) 99, 496
NdeII GATC 2 cut(s) 138, 264
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 2 cut(s) 328, 422
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 2 cut(s) 796, 807
NspI RCATGY 2 cut(s) 241, 743
PaeI GCATGC 1 cut(s) 241
PaeR7I CTCGAG 2 cut(s) 119, 134
PagI TCATGA 1 cut(s) 789
PceI AGGCCT 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 1 cut(s) 513
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 1 cut(s) 228
PkrI GCNGC 6 cut(s) 75, 115, 195, 198, 243, 312
PleI GAGTC 3 cut(s) 13, 22, 385
PpsI GAGTC 3 cut(s) 13, 22, 385
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 4 cut(s) 157, 228, 249, 727
PspGI CCWGG 4 cut(s) 157, 228, 249, 727
PspN4I GGNNCC 2 cut(s) 328, 422
PspPI GGNCC 4 cut(s) 155, 232, 326, 598
PspXI VCTCGAGB 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 401
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 4 cut(s) 317, 422, 494, 665
RsaNI GTAC 4 cut(s) 316, 421, 493, 664
RseI CAYNNNNRTG 2 cut(s) 560, 794
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 528, 675, 684
SatI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
Sau3AI GATC 2 cut(s) 138, 264
Sau96I GGNCC 4 cut(s) 155, 232, 326, 598
SchI GAGTC 3 cut(s) 13, 22, 385
ScrFI CCNGG 5 cut(s) 154, 159, 230, 251, 729
SduI GDGCHC 2 cut(s) 108, 126
SfaNI GCATC 3 cut(s) 424, 703, 769
Sfr274I CTCGAG 2 cut(s) 119, 134
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 119, 134
SmiMI CAYNNNNRTG 2 cut(s) 560, 794
SmlI CTYRAG 4 cut(s) 107, 119, 134, 575
SmoI CTYRAG 4 cut(s) 107, 119, 134, 575
SphI GCATGC 1 cut(s) 241
Sse9I AATT 3 cut(s) 453, 672, 783
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 4 cut(s) 179, 235, 283, 310
SspMI CTAG 2 cut(s) 77, 552
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 5 cut(s) 152, 157, 228, 249, 727
StyI CCWWGG 2 cut(s) 99, 496
TaqI TCGA 6 cut(s) 8, 120, 126, 135, 263, 690
TasI AATT 3 cut(s) 453, 672, 783
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 1 cut(s) 513
Tru1I TTAA 3 cut(s) 528, 675, 684
Tru9I TTAA 3 cut(s) 528, 675, 684
TscAI CASTG 2 cut(s) 508, 643
TseFI GTSAC 2 cut(s) 796, 807
TseI GCWGC 5 cut(s) 73, 113, 193, 196, 241
Tsp45I GTSAC 2 cut(s) 796, 807
TspDTI ATGAA 2 cut(s) 578, 806
TspGWI ACGGA 1 cut(s) 598
TspRI CASTG 2 cut(s) 508, 643
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 232
XagI CCTNNNNNAGG 1 cut(s) 392
XceI RCATGY 2 cut(s) 241, 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XhoI CTCGAG 2 cut(s) 119, 134
XspI CTAG 2 cut(s) 77, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.