MD07G1301100.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003088
Physical Location & Seq
Reverse (-)
35980116 .. 35980466
351 bp
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UTR
Exon/CDS
Intron
MD07G1301100.v1.1.491

Sequence Viewer

Length: 351 bp
ATGGGATCGACCGGAGAAGCTCAGACGACTCCAACCCAAGTCTCCGACGAAGAAACAAACCTCTTCGCCATGCAGCTAGTCAACGCCCCCTTTCTCCCCATAGTGCTCAAGGCAGCCCTCGAGCTCGACCTCCTCGAGATCATGGCGAAAGCCGGGCTTGGCACTTTCGTTTCTCCGACGGACTTAGCTTCGCAGTTGCCGACCAAGAACCCTGACGACCCCGTCATGCTGGACCGCATGATGCACCTCCTGGTCAGCTACTCCATCCTCACTTACTCCCTCAGCATGCTTCCCGACGACAATGTCGAGCGGTTGTACGGCCTCGGCCCCATCTGCAAGTTCTTGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

117

Amino Acids

12.65

Weight (kDa)

4.24

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 24 - 115 3.8e-13 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 221, 302
AccBSI CCGCTC 1 cut(s) 310
AciI CCGC 2 cut(s) 235, 310
AclWI GGATC 1 cut(s) 13
AfaI GTAC 1 cut(s) 317
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 5 cut(s) 20, 76, 124, 188, 258
AluI AGCT 5 cut(s) 20, 76, 124, 188, 258
Alw21I GWGCWC 2 cut(s) 108, 126
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 1 cut(s) 13
Ama87I CYCGRG 2 cut(s) 119, 134
AoxI GGCC 2 cut(s) 319, 325
ApeKI GCWGC 2 cut(s) 73, 113
AspS9I GGNCC 2 cut(s) 232, 326
AsuC2I CCSGG 1 cut(s) 154
AvaI CYCGRG 2 cut(s) 119, 134
AvaII GGWCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 108, 126
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 85, 125
BccI CCATC 2 cut(s) 272, 338
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 77
BisI GCNGC 2 cut(s) 74, 114
BlsI GCNGC 2 cut(s) 75, 115
Bme1390I CCNGG 2 cut(s) 154, 251
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 2 cut(s) 119, 134
BmgT120I GGNCC 2 cut(s) 232, 326
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 2 cut(s) 154, 251
BmsI GCATC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 11
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 35, 295
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 2 cut(s) 85, 125
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 2 cut(s) 321, 327
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 108, 126
BsiHKCI CYCGRG 2 cut(s) 119, 134
BsiSI CCGG 2 cut(s) 12, 153
BsmAI GTCTC 1 cut(s) 46
BsnI GGCC 2 cut(s) 321, 327
BsoBI CYCGRG 2 cut(s) 119, 134
Bsp1286I GDGCHC 2 cut(s) 108, 126
Bsp143I GATC 2 cut(s) 5, 138
BspACI CCGC 2 cut(s) 235, 310
BspANI GGCC 2 cut(s) 321, 327
BspCNI CTCAG 2 cut(s) 34, 294
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 2 cut(s) 5, 138
Bst2UI CCWGG 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 68
BstC8I GCNNGC 1 cut(s) 287
BstDEI CTNAG 3 cut(s) 21, 184, 281
BstF5I GGATG 1 cut(s) 264
BstKTI GATC 2 cut(s) 8, 141
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 2 cut(s) 5, 138
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 1 cut(s) 333
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 152, 249
BstV1I GCAGC 2 cut(s) 85, 125
BsuRI GGCC 2 cut(s) 321, 327
BtsCI GGATG 1 cut(s) 264
Cac8I GCNNGC 1 cut(s) 287
Cfr13I GGNCC 2 cut(s) 232, 326
Csp6I GTAC 1 cut(s) 316
CviAII CATG 5 cut(s) 70, 142, 226, 238, 286
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 3 cut(s) 21, 184, 281
DpnI GATC 2 cut(s) 7, 140
DpnII GATC 2 cut(s) 5, 138
DrdI GACNNNNNNGTC 2 cut(s) 221, 302
DseDI GACNNNNNNGTC 2 cut(s) 221, 302
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 119, 134
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 5 cut(s) 73, 145, 229, 241, 289
FaiI YATR 7 cut(s) 71, 101, 143, 227, 239, 287, 349
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FatI CATG 5 cut(s) 69, 141, 225, 237, 285
Fnu4HI GCNGC 2 cut(s) 74, 114
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 74, 114
FspBI CTAG 1 cut(s) 77
GluI GCNGC 2 cut(s) 74, 114
HaeIII GGCC 2 cut(s) 321, 327
HapII CCGG 2 cut(s) 12, 153
Hin1II CATG 5 cut(s) 73, 145, 229, 241, 289
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 1 cut(s) 28
HpaII CCGG 2 cut(s) 12, 153
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 3 cut(s) 24, 46, 177
Hpy188III TCNNGA 3 cut(s) 136, 293, 343
Hpy8I GTNNAC 1 cut(s) 82
Hpy99I CGWCG 3 cut(s) 50, 181, 299
HpyCH4V TGCA 3 cut(s) 73, 244, 336
HpyF10VI GCNNNNNNNGC 1 cut(s) 333
HpyF3I CTNAG 3 cut(s) 21, 184, 281
Hsp92II CATG 5 cut(s) 73, 145, 229, 241, 289
Kzo9I GATC 2 cut(s) 5, 138
LpnPI CCDG 6 cut(s) 25, 166, 215, 225, 236, 263
Lsp1109I GCAGC 2 cut(s) 85, 125
LweI GCATC 1 cut(s) 231
MaeI CTAG 1 cut(s) 77
MalI GATC 2 cut(s) 7, 140
MbiI CCGCTC 1 cut(s) 310
MboI GATC 2 cut(s) 5, 138
MboII GAAGA 2 cut(s) 55, 62
MhlI GDGCHC 2 cut(s) 108, 126
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 3 cut(s) 56, 69, 200
MnlI CCTC 8 cut(s) 71, 128, 140, 143, 257, 278, 290, 332
MspI CCGG 2 cut(s) 12, 153
MspR9I CCNGG 2 cut(s) 154, 251
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 333
NciI CCSGG 1 cut(s) 154
NdeII GATC 2 cut(s) 5, 138
NlaIII CATG 5 cut(s) 73, 145, 229, 241, 289
NlaIV GGNNCC 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 303
NspI RCATGY 1 cut(s) 289
PaeI GCATGC 1 cut(s) 289
PaeR7I CTCGAG 2 cut(s) 119, 134
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 197, 303
PflFI GACNNNGTC 2 cut(s) 221, 302
PkrI GCNGC 2 cut(s) 75, 115
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 232, 326
PspXI VCTCGAGB 1 cut(s) 119
PsyI GACNNNGTC 2 cut(s) 221, 302
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 2 cut(s) 74, 114
Sau3AI GATC 2 cut(s) 5, 138
Sau96I GGNCC 2 cut(s) 232, 326
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 2 cut(s) 154, 251
SduI GDGCHC 2 cut(s) 108, 126
SetI ASST 8 cut(s) 22, 63, 78, 126, 132, 190, 249, 260
SfaNI GCATC 1 cut(s) 231
Sfr274I CTCGAG 2 cut(s) 119, 134
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 119, 134
SmlI CTYRAG 3 cut(s) 107, 119, 134
SmoI CTYRAG 3 cut(s) 107, 119, 134
SphI GCATGC 1 cut(s) 289
SsiI CCGC 2 cut(s) 235, 310
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 152, 249
TaqI TCGA 5 cut(s) 8, 120, 126, 135, 306
TseI GCWGC 2 cut(s) 73, 113
TspGWI ACGGA 1 cut(s) 194
Tth111I GACNNNGTC 2 cut(s) 221, 302
VpaK11BI GGWCC 1 cut(s) 232
XceI RCATGY 1 cut(s) 289
XhoI CTCGAG 2 cut(s) 119, 134
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.