pycom07g27350
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003088
Physical Location & Seq
Reverse (-)
27112991 .. 27115969
2979 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27350.3

Sequence Viewer

Length: 1074 bp
ATGGGTTCGACCGGAAAGACTCAGATGACTCCAACCCAAGTCTCCGAGCAAGAAACAAACCTCTTCGCCATGCAGCTAGCCAACGCCCCCGTTCTCCCCATGGTGCTTAAGACAGCCCTCGAGCTCGACCTCCTCGAGATCATGGCGAAAGCCGGGCCTGGCGCTTTCGTTTCTCCGGCAGACTTAGCTTCGCAGCTGCCGACCAAGAACCCTGACGCCCCCGTCATGCTGGACCGCATGCTGCGCCTCCTGGCCAGCTACTCCGTCCTCACTTACTCCCTCCGCACGCTACCCGACGGCAATGTCGAGCGGCTGTACGGCCTCAGCCCCGTATGCAAGCTCTTGACAAAGAACGGGGATGGTGTTTCCTTTGCTCCTCTCTGCGTCATGAGTCAGGACAGGGTCTTTATGGAGAGCTGGTACCACTTGAAAGATGCAGTTCTTGAAGGAGGAATTCCATTCAACAAGGCCTTTGGAATGACTGCTTTTGAGTACCCTGGCACTGACCCTAGATTCAACAAGGTCTTCAACAGGGGAATGGCTGTCCAATCTACCATTATCATGGAAAAACTTCTAGAGACCTACAAGGGCTTTGAGGGCCTCACATCTGTCGTAGATGTTGGCGGTGGCACTGGCGCTGCTGTTAACATGATCGTTTCTAAGTACCCCTCGATTAAGGGTATCAACTTCGACTTGCCTCATGTCATAGACGATGCTCCCCAATTTCTTGGTGTGGAGCATGTTGGAGGAGACATGTTTGCAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGGTGATTCTTGCTGAGGGCATTCTTCCAGTAGCTCCAGACAGCAGCCTTGCCACCAAGGTAGTTGTCAATCTCGACGTGATCATGTTGGGTTACAACCCCGGAGGAAAAGAGCGGACGGAGAAGGAGTTTGAGGCCTTGGCTCCGATCGATCTGGATTCAAAGGCTTCCGCGTCATCTGCTCCGCTTTCCACATCTATGCCATTGAGTTTCTTAAGAAGATTTGAGTATAACTTTGGCAGTGGTATGCTGCCGTCTGCCTTCATATTGCTTTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

358

Amino Acids

38.48

Weight (kDa)

5.05

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 221, 302
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 1 cut(s) 420
AccBSI CCGCTC 2 cut(s) 310, 907
AccII CGCG 1 cut(s) 965
AciI CCGC 7 cut(s) 235, 283, 310, 624, 907, 963, 977
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 4 cut(s) 317, 422, 494, 665
AflII CTTAAG 2 cut(s) 107, 1006
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 6 cut(s) 430, 446, 463, 517, 529, 954
AjiI CACGTC 1 cut(s) 871
AjnI CCWGG 3 cut(s) 157, 249, 496
AluBI AGCT 8 cut(s) 76, 124, 188, 196, 258, 340, 417, 827
AluI AGCT 8 cut(s) 76, 124, 188, 196, 258, 340, 417, 827
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 46, 572, 744
Ama87I CYCGRG 2 cut(s) 119, 134
AoxI GGCC 6 cut(s) 155, 252, 319, 468, 598, 927
ApeKI GCWGC 7 cut(s) 73, 193, 196, 241, 638, 837, 1042
ApoI RAATTY 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 420
AspLEI GCGC 3 cut(s) 164, 246, 638
AspS9I GGNCC 3 cut(s) 155, 232, 598
AsuC2I CCSGG 2 cut(s) 154, 894
AsuHPI GGTGA 1 cut(s) 808
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 119, 134
AvaII GGWCC 1 cut(s) 232
BalI TGGCCA 1 cut(s) 254
BanI GGYRCC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 2 cut(s) 323, 807
BbvI GCAGC 7 cut(s) 85, 183, 205, 228, 625, 849, 1029
BccI CCATC 1 cut(s) 353
BceAI ACGGC 3 cut(s) 313, 334, 1030
BciT130I CCWGG 3 cut(s) 159, 251, 498
BclI TGATCA 1 cut(s) 873
BcnI CCSGG 2 cut(s) 154, 894
BcoDI GTCTC 3 cut(s) 46, 572, 744
BfaI CTAG 3 cut(s) 77, 510, 575
BfoI RGCGCY 2 cut(s) 165, 639
BfrI CTTAAG 2 cut(s) 107, 1006
BisI GCNGC 8 cut(s) 74, 194, 197, 242, 311, 639, 838, 1043
BlsI GCNGC 8 cut(s) 75, 195, 198, 243, 312, 640, 839, 1044
Bme1390I CCNGG 5 cut(s) 154, 159, 251, 498, 894
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 2 cut(s) 119, 134
BmgBI CACGTC 1 cut(s) 871
BmgT120I GGNCC 3 cut(s) 155, 232, 598
BmiI GGNNCC 2 cut(s) 422, 936
BmrFI CCNGG 5 cut(s) 154, 159, 251, 498, 894
BmsI GCATC 3 cut(s) 424, 703, 769
BmtI GCTAGC 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 2 cut(s) 323, 807
BpuMI CCSGG 2 cut(s) 154, 894
Bsa29I ATCGAT 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 5 cut(s) 99, 496, 849, 892, 930
BsaWI WCCGGW 1 cut(s) 11
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 905, 935
Bse1I ACTGG 2 cut(s) 637, 821
Bse3DI GCAATG 1 cut(s) 307
BseBI CCWGG 3 cut(s) 159, 251, 498
BseCI ATCGAT 1 cut(s) 942
BseDI CCNNGG 5 cut(s) 99, 496, 849, 892, 930
BseGI GGATG 1 cut(s) 364
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 3 cut(s) 35, 337, 798
BseNI ACTGG 2 cut(s) 637, 821
BseRI GAGGAG 3 cut(s) 122, 366, 762
BseXI GCAGC 7 cut(s) 85, 183, 205, 228, 625, 849, 1029
Bsh1236I CGCG 1 cut(s) 965
Bsh1285I CGRYCG 2 cut(s) 12, 942
BshFI GGCC 6 cut(s) 157, 254, 321, 470, 600, 929
BshNI GGYRCC 1 cut(s) 420
BshVI ATCGAT 1 cut(s) 942
BsiEI CGRYCG 2 cut(s) 12, 942
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 2 cut(s) 119, 134
BsiSI CCGG 4 cut(s) 12, 153, 176, 894
BsmAI GTCTC 3 cut(s) 46, 572, 744
BsmI GAATGC 1 cut(s) 813
BsnI GGCC 6 cut(s) 157, 254, 321, 470, 600, 929
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 2 cut(s) 119, 134
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 5 cut(s) 138, 651, 873, 939, 943
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 7 cut(s) 235, 283, 310, 624, 907, 963, 977
BspANI GGCC 6 cut(s) 157, 254, 321, 470, 600, 929
BspCNI CTCAG 3 cut(s) 34, 336, 799
BspDI ATCGAT 1 cut(s) 942
BspFNI CGCG 1 cut(s) 965
BspHI TCATGA 2 cut(s) 387, 789
BspLI GGNNCC 2 cut(s) 422, 936
BspOI GCTAGC 1 cut(s) 80
BspT107I GGYRCC 1 cut(s) 420
BspTI CTTAAG 2 cut(s) 107, 1006
BspTNI GGTCTC 1 cut(s) 572
BsrBI CCGCTC 2 cut(s) 310, 907
BsrDI GCAATG 1 cut(s) 307
BsrI ACTGG 2 cut(s) 637, 821
BssECI CCNNGG 5 cut(s) 99, 496, 849, 892, 930
BssMI GATC 5 cut(s) 138, 651, 873, 939, 943
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 3 cut(s) 99, 849, 930
Bst2UI CCWGG 3 cut(s) 159, 251, 498
Bst6I CTCTTC 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 216
BstAFI CTTAAG 2 cut(s) 107, 1006
BstC8I GCNNGC 5 cut(s) 78, 239, 256, 287, 338
BstDEI CTNAG 5 cut(s) 21, 184, 323, 660, 807
BstDSI CCRYGG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 965
BstH2I RGCGCY 2 cut(s) 165, 639
BstHHI GCGC 3 cut(s) 164, 246, 638
BstKTI GATC 5 cut(s) 141, 654, 876, 942, 946
BstMAI GTCTC 3 cut(s) 46, 572, 744
BstMBI GATC 5 cut(s) 138, 651, 873, 939, 943
BstMCI CGRYCG 2 cut(s) 12, 942
BstMWI GCNNNNNNNGC 5 cut(s) 185, 243, 333, 597, 971
BstNI CCWGG 3 cut(s) 159, 251, 498
BstNSI RCATGY 3 cut(s) 241, 743, 757
BstSCI CCNGG 5 cut(s) 152, 157, 249, 496, 892
BstUI CGCG 1 cut(s) 965
BstV1I GCAGC 7 cut(s) 85, 183, 205, 228, 625, 849, 1029
BstV2I GAAGAC 1 cut(s) 517
BstXI CCANNNNNNTGG 2 cut(s) 562, 728
Bsu15I ATCGAT 1 cut(s) 942
BsuRI GGCC 6 cut(s) 157, 254, 321, 470, 600, 929
BsuTUI ATCGAT 1 cut(s) 942
BtgI CCRYGG 1 cut(s) 99
BtrI CACGTC 1 cut(s) 871
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 1039
BtsIMutI CAGTG 3 cut(s) 501, 630, 1039
Cac8I GCNNGC 5 cut(s) 78, 239, 256, 287, 338
CciI TCATGA 2 cut(s) 387, 789
CfoI GCGC 3 cut(s) 164, 246, 638
Cfr13I GGNCC 3 cut(s) 155, 232, 598
ClaI ATCGAT 1 cut(s) 942
CseI GACGC 3 cut(s) 224, 373, 954
Csp6I GTAC 4 cut(s) 316, 421, 493, 664
CviQI GTAC 4 cut(s) 316, 421, 493, 664
DdeI CTNAG 5 cut(s) 21, 184, 323, 660, 807
DpnI GATC 5 cut(s) 140, 653, 875, 941, 945
DpnII GATC 5 cut(s) 138, 651, 873, 939, 943
DrdI GACNNNNNNGTC 2 cut(s) 221, 302
DseDI GACNNNNNNGTC 2 cut(s) 221, 302
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 3 cut(s) 99, 849, 930
Eco147I AGGCCT 2 cut(s) 470, 929
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 119, 134
EcoICRI GAGCTC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 3 cut(s) 157, 249, 496
EcoT14I CCWWGG 3 cut(s) 99, 849, 930
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 3 cut(s) 99, 849, 930
FbaI TGATCA 1 cut(s) 873
Fnu4HI GCNGC 8 cut(s) 74, 194, 197, 242, 311, 639, 838, 1043
FokI GGATG 1 cut(s) 371
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 8 cut(s) 74, 194, 197, 242, 311, 639, 838, 1043
FspBI CTAG 3 cut(s) 77, 510, 575
GlaI GCGC 3 cut(s) 163, 245, 637
GluI GCNGC 8 cut(s) 74, 194, 197, 242, 311, 639, 838, 1043
GsuI CTGGAG 1 cut(s) 813
HaeII RGCGCY 2 cut(s) 165, 639
HaeIII GGCC 6 cut(s) 157, 254, 321, 470, 600, 929
HapII CCGG 4 cut(s) 12, 153, 176, 894
HgaI GACGC 3 cut(s) 224, 373, 954
HhaI GCGC 3 cut(s) 164, 246, 638
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 3 cut(s) 162, 244, 636
HinP1I GCGC 3 cut(s) 162, 244, 636
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 6 cut(s) 19, 28, 391, 513, 799, 950
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 4 cut(s) 12, 153, 176, 894
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 3 cut(s) 24, 46, 939
Hpy8I GTNNAC 1 cut(s) 646
Hpy99I CGWCG 2 cut(s) 299, 872
HpyAV CCTTC 4 cut(s) 440, 787, 910, 1063
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 5 cut(s) 73, 336, 437, 761, 782
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 243, 333, 597, 971
HpyF3I CTNAG 5 cut(s) 21, 184, 323, 660, 807
HpySE526I ACGT 1 cut(s) 870
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 3 cut(s) 162, 244, 636
KpnI GGTACC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 873
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 5 cut(s) 138, 651, 873, 939, 943
LmnI GCTCC 6 cut(s) 379, 721, 736, 832, 940, 979
Lsp1109I GCAGC 7 cut(s) 85, 183, 205, 228, 625, 849, 1029
LweI GCATC 3 cut(s) 424, 703, 769
MaeI CTAG 3 cut(s) 77, 510, 575
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 1 cut(s) 884
MalI GATC 5 cut(s) 140, 653, 875, 941, 945
MbiI CCGCTC 2 cut(s) 310, 907
MboI GATC 5 cut(s) 138, 651, 873, 939, 943
MboII GAAGA 4 cut(s) 55, 517, 809, 1023
MhlI GDGCHC 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 3 cut(s) 453, 722, 783
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 3 cut(s) 13, 22, 400
MmeI TCCRAC 2 cut(s) 56, 724
Mox20I TGGCCA 1 cut(s) 254
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 1 cut(s) 254
MseI TTAA 4 cut(s) 108, 645, 675, 1007
MslI CAYNNNNRTG 3 cut(s) 560, 794, 989
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 196
MspCI CTTAAG 2 cut(s) 107, 1006
MspI CCGG 4 cut(s) 12, 153, 176, 894
MspR9I CCNGG 5 cut(s) 154, 159, 251, 498, 894
Mva1269I GAATGC 1 cut(s) 813
MvaI CCWGG 3 cut(s) 159, 251, 498
MvnI CGCG 1 cut(s) 965
MwoI GCNNNNNNNGC 5 cut(s) 185, 243, 333, 597, 971
NciI CCSGG 2 cut(s) 154, 894
NcoI CCATGG 1 cut(s) 99
NdeII GATC 5 cut(s) 138, 651, 873, 939, 943
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 2 cut(s) 422, 936
NspI RCATGY 3 cut(s) 241, 743, 757
PaeI GCATGC 1 cut(s) 241
PaeR7I CTCGAG 2 cut(s) 119, 134
PagI TCATGA 2 cut(s) 387, 789
PceI AGGCCT 2 cut(s) 470, 929
PciI ACATGT 1 cut(s) 753
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 197, 303
PctI GAATGC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 3 cut(s) 513, 799, 950
PflFI GACNNNGTC 1 cut(s) 401
PkrI GCNGC 8 cut(s) 75, 195, 198, 243, 312, 640, 839, 1044
Ple19I CGATCG 1 cut(s) 942
PleI GAGTC 3 cut(s) 13, 22, 399
PpsI GAGTC 3 cut(s) 13, 22, 399
PscI ACATGT 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 3 cut(s) 157, 249, 496
PspGI CCWGG 3 cut(s) 157, 249, 496
PspN4I GGNNCC 2 cut(s) 422, 936
PspPI GGNCC 3 cut(s) 155, 232, 598
PspXI VCTCGAGB 1 cut(s) 119
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 942
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 4 cut(s) 317, 422, 494, 665
RsaNI GTAC 4 cut(s) 316, 421, 493, 664
RseI CAYNNNNRTG 3 cut(s) 560, 794, 989
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 4 cut(s) 108, 645, 675, 1007
SatI GCNGC 8 cut(s) 74, 194, 197, 242, 311, 639, 838, 1043
Sau3AI GATC 5 cut(s) 138, 651, 873, 939, 943
Sau96I GGNCC 3 cut(s) 155, 232, 598
SchI GAGTC 3 cut(s) 13, 22, 400
ScrFI CCNGG 5 cut(s) 154, 159, 251, 498, 894
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 3 cut(s) 424, 703, 769
Sfr274I CTCGAG 2 cut(s) 119, 134
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 119, 134
SmiMI CAYNNNNRTG 3 cut(s) 560, 794, 989
SmlI CTYRAG 4 cut(s) 107, 119, 134, 1006
SmoI CTYRAG 4 cut(s) 107, 119, 134, 1006
SphI GCATGC 1 cut(s) 241
Sse9I AATT 3 cut(s) 453, 722, 783
SseBI AGGCCT 2 cut(s) 470, 929
SsiI CCGC 7 cut(s) 235, 283, 310, 624, 907, 963, 977
SspMI CTAG 3 cut(s) 77, 510, 575
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 2 cut(s) 470, 929
StyD4I CCNGG 5 cut(s) 152, 157, 249, 496, 892
StyI CCWWGG 3 cut(s) 99, 849, 930
TaiI ACGT 1 cut(s) 873
TaqI TCGA 9 cut(s) 8, 120, 126, 135, 306, 671, 690, 867, 942
TasI AATT 3 cut(s) 453, 722, 783
TauI GCSGC 1 cut(s) 313
TfiI GAWTC 3 cut(s) 513, 799, 950
Tru1I TTAA 4 cut(s) 108, 645, 675, 1007
Tru9I TTAA 4 cut(s) 108, 645, 675, 1007
TscAI CASTG 3 cut(s) 508, 637, 1039
TseI GCWGC 7 cut(s) 73, 193, 196, 241, 638, 837, 1042
TspDTI ATGAA 3 cut(s) 778, 806, 1045
TspGWI ACGGA 2 cut(s) 253, 926
TspRI CASTG 3 cut(s) 508, 637, 1039
Tth111I GACNNNGTC 1 cut(s) 401
Vha464I CTTAAG 2 cut(s) 107, 1006
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 453
XbaI TCTAGA 1 cut(s) 574
XceI RCATGY 3 cut(s) 241, 743, 757
XhoI CTCGAG 2 cut(s) 119, 134
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 3 cut(s) 77, 510, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.