MD07G1209500.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003088
Physical Location & Seq
Reverse (-)
28874294 .. 28875228
935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1209500.v1.1.491

Sequence Viewer

Length: 450 bp
ATGATCGTTTCTAAGTACCCCTCGATTAAGGGTATTAACTTCGACCTGCCTCATGTCATAAAAGATGCTCCCCAATATCCTGGTGTGGAGCATGTTGGAGGAGACATGTTTGTAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGTTTATATGTCACGACTGGAGTGACGATCATTGCTTGAAATTTTTGAAGAACTGTTATACCGCGGTCCCTGACAATGGGAAGGTGATTCTTGCTGAGTGCATTCTTCCAGTAGCTCCAGACAGCAGCCTTGCCACCAAGGGAGTTGTCCATATCGACGTGATCATGTTAGCTCACTACCCCGGAGGAAAAGAGCGGACGGAGAAGGAGTTTGAGGCCTTGGCTAAGGGATCTGGATTCAAAGGTTTCCGCGTCATCTGCTCCGCTTTCAACACCTATGTCATTGAGTTTCTTAAGAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

150

Amino Acids

16.48

Weight (kDa)

6.99

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 2 - 129 7.8e-47 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 54
AccBSI CCGCTC 1 cut(s) 340
AccII CGCG 2 cut(s) 209, 396
AciI CCGC 5 cut(s) 207, 209, 340, 394, 408
AclWI GGATC 1 cut(s) 382
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 221
AflII CTTAAG 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 4 cut(s) 184, 193, 385, 415
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 2 cut(s) 260, 317
AluI AGCT 2 cut(s) 260, 317
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 1 cut(s) 382
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 1 cut(s) 270
ApoI RAATTY 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 211
BbvI GCAGC 1 cut(s) 282
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 306
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 54
BisI GCNGC 1 cut(s) 271
BlsI GCNGC 1 cut(s) 272
Bme1390I CCNGG 2 cut(s) 81, 327
Bme18I GGWCC 1 cut(s) 211
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 2 cut(s) 81, 327
BmsI GCATC 2 cut(s) 55, 121
BpmI CTGGAG 2 cut(s) 184, 246
Bpu10I CCTNAGC 1 cut(s) 369
BpuMI CCSGG 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 207, 282, 325, 363
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 2 cut(s) 167, 254
Bse3DI GCAATG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 4 cut(s) 207, 282, 325, 363
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 167, 254
BseRI GAGGAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 282
Bsh1236I CGCG 2 cut(s) 209, 396
BshFI GGCC 1 cut(s) 362
BsiSI CCGG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 246
BsnI GGCC 1 cut(s) 362
Bsp143I GATC 4 cut(s) 3, 172, 306, 374
BspACI CCGC 5 cut(s) 207, 209, 340, 394, 408
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 1 cut(s) 232
BspFNI CGCG 2 cut(s) 209, 396
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 54
BspPI GGATC 1 cut(s) 382
BspTI CTTAAG 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 175
BsrI ACTGG 2 cut(s) 167, 254
BssECI CCNNGG 4 cut(s) 207, 282, 325, 363
BssMI GATC 4 cut(s) 3, 172, 306, 374
BssT1I CCWWGG 2 cut(s) 282, 363
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 200
BstAFI CTTAAG 1 cut(s) 437
BstDEI CTNAG 3 cut(s) 12, 240, 369
BstDSI CCRYGG 1 cut(s) 207
BstFNI CGCG 2 cut(s) 209, 396
BstKTI GATC 4 cut(s) 6, 175, 309, 377
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 4 cut(s) 3, 172, 306, 374
BstMWI GCNNNNNNNGC 1 cut(s) 402
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 2 cut(s) 95, 109
BstSCI CCNGG 2 cut(s) 79, 325
BstUI CGCG 2 cut(s) 209, 396
BstV1I GCAGC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 1 cut(s) 374
BsuRI GGCC 1 cut(s) 362
BtgI CCRYGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 304
BveI ACCTGC 1 cut(s) 54
CciI TCATGA 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 211
Cfr42I CCGCGG 1 cut(s) 210
CseI GACGC 1 cut(s) 385
Csp6I GTAC 1 cut(s) 16
CviAII CATG 5 cut(s) 53, 92, 106, 142, 310
CviJI RGCY 5 cut(s) 260, 273, 317, 362, 368
CviKI_1 RGCY 5 cut(s) 260, 273, 317, 362, 368
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 3 cut(s) 12, 240, 369
DpnI GATC 4 cut(s) 5, 174, 308, 376
DpnII GATC 4 cut(s) 3, 172, 306, 374
Eco130I CCWWGG 2 cut(s) 282, 363
Eco147I AGGCCT 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 282, 363
ErhI CCWWGG 2 cut(s) 282, 363
FaeI CATG 5 cut(s) 56, 95, 109, 145, 313
FaqI GGGAC 1 cut(s) 197
FatI CATG 5 cut(s) 52, 91, 105, 141, 309
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 271
GluI GCNGC 1 cut(s) 271
GsuI CTGGAG 2 cut(s) 184, 246
HaeIII GGCC 1 cut(s) 362
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 385
Hin1II CATG 5 cut(s) 56, 95, 109, 145, 313
HinfI GANTC 2 cut(s) 232, 381
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 241
Hpy188III TCNNGA 4 cut(s) 142, 158, 263, 378
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 220, 343
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 2 cut(s) 134, 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 402
HpyF3I CTNAG 3 cut(s) 12, 240, 369
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 5 cut(s) 56, 95, 109, 145, 313
Ksp22I TGATCA 1 cut(s) 306
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 4 cut(s) 3, 172, 306, 374
LmnI GCTCC 4 cut(s) 73, 88, 265, 410
LpnPI CCDG 9 cut(s) 59, 66, 93, 148, 228, 267, 276, 340, 363
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 2 cut(s) 55, 121
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 155, 167
MalI GATC 4 cut(s) 5, 174, 308, 376
MbiI CCGCTC 1 cut(s) 340
MboI GATC 4 cut(s) 3, 172, 306, 374
MboII GAAGA 2 cut(s) 205, 242
MflI RGATCY 1 cut(s) 374
MluCI AATT 2 cut(s) 135, 185
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 5 cut(s) 31, 60, 92, 323, 352
MseI TTAA 3 cut(s) 27, 36, 438
MspA1I CMGCKG 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 437
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 81, 327
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 2 cut(s) 209, 396
MwoI GCNNNNNNNGC 1 cut(s) 402
NciI CCSGG 1 cut(s) 327
NdeII GATC 4 cut(s) 3, 172, 306, 374
NlaIII CATG 5 cut(s) 56, 95, 109, 145, 313
NlaIV GGNNCC 1 cut(s) 213
NmuCI GTSAC 2 cut(s) 155, 167
NspI RCATGY 2 cut(s) 95, 109
PagI TCATGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 362
PciI ACATGT 1 cut(s) 105
PctI GAATGC 1 cut(s) 246
PfeI GAWTC 2 cut(s) 232, 381
PkrI GCNGC 1 cut(s) 272
PscI ACATGT 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 211
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 3 cut(s) 27, 36, 438
SatI GCNGC 1 cut(s) 271
Sau3AI GATC 4 cut(s) 3, 172, 306, 374
Sau96I GGNCC 1 cut(s) 211
ScrFI CCNGG 2 cut(s) 81, 327
SetI ASST 7 cut(s) 48, 231, 262, 306, 319, 391, 422
SfaNI GCATC 2 cut(s) 55, 121
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SinI GGWCC 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 2 cut(s) 135, 185
SseBI AGGCCT 1 cut(s) 362
SsiI CCGC 5 cut(s) 207, 209, 340, 394, 408
StuI AGGCCT 1 cut(s) 362
StyD4I CCNGG 2 cut(s) 79, 325
StyI CCWWGG 2 cut(s) 282, 363
TaaI ACNGT 1 cut(s) 200
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 23, 42, 300
TasI AATT 2 cut(s) 135, 185
TfiI GAWTC 2 cut(s) 232, 381
Tru1I TTAA 3 cut(s) 27, 36, 438
Tru9I TTAA 3 cut(s) 27, 36, 438
TseFI GTSAC 2 cut(s) 155, 167
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 2 cut(s) 155, 167
TspDTI ATGAA 2 cut(s) 130, 158
TspGWI ACGGA 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 185
XceI RCATGY 2 cut(s) 95, 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.