MD07G1300300.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003088
Physical Location & Seq
Reverse (-)
35924007 .. 35925002
996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1300300.v1.1.491

Sequence Viewer

Length: 213 bp
ATGATCATTTCTAAGTACCCCTCGATTAAGGGTATTAATTTCGACTTGCCTCATATCATAGAAGATGTTCCCCAATATTCTGGTGAAGAGCATGTTGGAGGAGACATGTTTGTAAGTGTTCCAAAGGGAGATGCAATTTTCATGAAGAACTGCTATACCGCGCTACCCGACAATGGGAAGGTGATTCTTGCTGAATGTGCTTTCTTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

71

Amino Acids

7.75

Weight (kDa)

4.78

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_2 PF00891 2 - 49 1.3e-11 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 161
AciI CCGC 1 cut(s) 159
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 2 cut(s) 173, 174
AflIII ACRYGT 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
Alw26I GTCTC 1 cut(s) 96
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 66
AspLEI GCGC 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 95, 193
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 96
BmsI GCATC 1 cut(s) 121
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 174
Bse1I ACTGG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 173, 174
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 114
Bsh1236I CGCG 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 173, 174
BsmAI GTCTC 1 cut(s) 96
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 159
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 81
BsrI ACTGG 1 cut(s) 208
BssMI GATC 1 cut(s) 3
Bst6I CTCTTC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 12
BstFNI CGCG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 2 cut(s) 95, 109
BstUI CGCG 1 cut(s) 161
BstXI CCANNNNNNTGG 1 cut(s) 80
CciI TCATGA 1 cut(s) 141
CfoI GCGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 16
CviAII CATG 3 cut(s) 92, 106, 142
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 1 cut(s) 12
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
FaeI CATG 3 cut(s) 95, 109, 145
FaiI YATR 6 cut(s) 54, 59, 93, 107, 143, 156
FatI CATG 3 cut(s) 91, 105, 141
FbaI TGATCA 1 cut(s) 3
GlaI GCGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 163
Hin1II CATG 3 cut(s) 95, 109, 145
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HinfI GANTC 1 cut(s) 184
HphI GGTGA 2 cut(s) 95, 193
Hpy188III TCNNGA 1 cut(s) 142
HpyAV CCTTC 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 1 cut(s) 12
Hsp92II CATG 3 cut(s) 95, 109, 145
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LguI GCTCTTC 1 cut(s) 81
LpnPI CCDG 1 cut(s) 66
LweI GCATC 1 cut(s) 121
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 4 cut(s) 74, 98, 157, 196
MluCI AATT 2 cut(s) 37, 135
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 3 cut(s) 31, 60, 92
MroXI GAANNNNTTC 1 cut(s) 66
MseI TTAA 2 cut(s) 27, 36
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeII GATC 1 cut(s) 3
NlaIII CATG 3 cut(s) 95, 109, 145
NspI RCATGY 2 cut(s) 95, 109
PagI TCATGA 1 cut(s) 141
PciI ACATGT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 66
PfeI GAWTC 1 cut(s) 184
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 1 cut(s) 36
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 27, 36
Sau3AI GATC 1 cut(s) 3
SetI ASST 1 cut(s) 183
SfaNI GCATC 1 cut(s) 121
SgeI CNNG 9 cut(s) 34, 58, 93, 104, 118, 154, 172, 179, 200
Sse9I AATT 2 cut(s) 37, 135
SsiI CCGC 1 cut(s) 159
SspI AATATT 1 cut(s) 77
TaqI TCGA 2 cut(s) 23, 42
TasI AATT 2 cut(s) 37, 135
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 2 cut(s) 27, 36
Tru9I TTAA 2 cut(s) 27, 36
TspDTI ATGAA 2 cut(s) 130, 158
VspI ATTAAT 1 cut(s) 36
XceI RCATGY 2 cut(s) 95, 109
XmnI GAANNNNTTC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.