pycom07g27320
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003088
Physical Location & Seq
Reverse (-)
27089453 .. 27090159
707 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g27320.1

Sequence Viewer

Length: 450 bp
ATGCGTTCGACCAGAGAGACTCAGATGACTCCAACCCAAGTCTCCGACGAAGAAACCAACCTCGATGCCATGCAGCTAGCCTCCGCCTCCGTTCCCCCCATGGTGCTCAAGGCAGCCCTCGAGCTCGACCTCCTCGAGATCATGGCGAAAGCCGGGCCTGGCGCTTTCGTTTCTCCGGAGGACTTAGCTTCGCAGCTGCCGACCAGGAACCCTGACGCCCCCGTCATTCTGGACCGCAAGCTGCGCCTCCTGGCCAGCTACTCCATCCTCACTTACTCCCTCCGCACGCTACCCGATGGCAAAGTAGAGCGGCTGTACGGCCTCGGCCCCGTCTGCAAGTTATTGACAAAGAACGAAGATGGTTTGTCCATTGCTCCTCACTTACTCCTGACTCAGGACAAGTGTTTCAATTTCAAACCTTTCTTTCTTATTAATCTTTTTCCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00950 ko01100 ko01110 map00940 map00950 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

150

Amino Acids

16.44

Weight (kDa)

5.39

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 24 - 117 1.8e-14 O-methyltransferase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccBSI CCGCTC 1 cut(s) 310
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 4 cut(s) 84, 235, 283, 310
AcoI YGGCCR 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 2 cut(s) 409, 415
AjnI CCWGG 3 cut(s) 157, 203, 249
AluBI AGCT 6 cut(s) 76, 124, 188, 196, 241, 258
AluI AGCT 6 cut(s) 76, 124, 188, 196, 241, 258
Alw21I GWGCWC 2 cut(s) 108, 126
Alw26I GTCTC 2 cut(s) 11, 46
Ama87I CYCGRG 2 cut(s) 119, 134
Aor13HI TCCGGA 1 cut(s) 175
AoxI GGCC 4 cut(s) 155, 252, 319, 325
ApeKI GCWGC 5 cut(s) 73, 113, 193, 196, 241
AseI ATTAAT 1 cut(s) 432
AspLEI GCGC 2 cut(s) 164, 246
AspS9I GGNCC 3 cut(s) 155, 232, 326
AsuC2I CCSGG 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 76
AvaI CYCGRG 2 cut(s) 119, 134
AvaII GGWCC 1 cut(s) 232
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 2 cut(s) 108, 126
BbvI GCAGC 5 cut(s) 85, 125, 183, 205, 228
BccI CCATC 3 cut(s) 272, 290, 353
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 3 cut(s) 159, 205, 251
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 11, 46
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
BlsI GCNGC 6 cut(s) 75, 115, 195, 198, 243, 312
Bme1390I CCNGG 4 cut(s) 154, 159, 205, 251
Bme18I GGWCC 1 cut(s) 232
BmeT110I CYCGRG 2 cut(s) 119, 134
BmgT120I GGNCC 3 cut(s) 155, 232, 326
BmiI GGNNCC 2 cut(s) 209, 328
BmrFI CCNGG 4 cut(s) 154, 159, 205, 251
BmsI GCATC 1 cut(s) 55
BmtI GCTAGC 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 99, 322
BsaWI WCCGGW 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 175
BseBI CCWGG 3 cut(s) 159, 205, 251
BseDI CCNNGG 2 cut(s) 99, 322
BseGI GGATG 1 cut(s) 264
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 35, 407
BseRI GAGGAG 2 cut(s) 122, 366
BseXI GCAGC 5 cut(s) 85, 125, 183, 205, 228
BshFI GGCC 4 cut(s) 157, 254, 321, 327
BsiHKAI GWGCWC 2 cut(s) 108, 126
BsiHKCI CYCGRG 2 cut(s) 119, 134
BsiSI CCGG 2 cut(s) 153, 176
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 2 cut(s) 11, 46
BsnI GGCC 4 cut(s) 157, 254, 321, 327
BsoBI CYCGRG 2 cut(s) 119, 134
Bsp1286I GDGCHC 2 cut(s) 108, 126
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 99
BspACI CCGC 4 cut(s) 84, 235, 283, 310
BspANI GGCC 4 cut(s) 157, 254, 321, 327
BspCNI CTCAG 2 cut(s) 34, 406
BspEI TCCGGA 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 209, 328
BspOI GCTAGC 1 cut(s) 80
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 369
BssECI CCNNGG 2 cut(s) 99, 322
BssMI GATC 1 cut(s) 138
BssNI GRCGYC 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 3 cut(s) 159, 205, 251
BstACI GRCGYC 1 cut(s) 216
BstC8I GCNNGC 4 cut(s) 78, 239, 256, 287
BstDEI CTNAG 3 cut(s) 21, 184, 393
BstDSI CCRYGG 1 cut(s) 99
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 164, 246
BstKTI GATC 1 cut(s) 141
BstMAI GTCTC 2 cut(s) 11, 46
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 243, 333
BstNI CCWGG 3 cut(s) 159, 205, 251
BstSCI CCNGG 4 cut(s) 152, 157, 203, 249
BstV1I GCAGC 5 cut(s) 85, 125, 183, 205, 228
BsuRI GGCC 4 cut(s) 157, 254, 321, 327
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 1 cut(s) 264
Cac8I GCNNGC 4 cut(s) 78, 239, 256, 287
CfoI GCGC 2 cut(s) 164, 246
Cfr13I GGNCC 3 cut(s) 155, 232, 326
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 316
CviAII CATG 3 cut(s) 70, 100, 142
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 3 cut(s) 21, 184, 393
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 252
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 2 cut(s) 119, 134
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoRII CCWGG 3 cut(s) 157, 203, 249
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 3 cut(s) 73, 103, 145
FaiI YATR 3 cut(s) 71, 101, 143
FatI CATG 3 cut(s) 69, 99, 141
Fnu4HI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
FspBI CTAG 1 cut(s) 77
GlaI GCGC 2 cut(s) 163, 245
GluI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 157, 254, 321, 327
HapII CCGG 2 cut(s) 153, 176
HgaI GACGC 1 cut(s) 224
HhaI GCGC 2 cut(s) 164, 246
Hin1I GRCGYC 1 cut(s) 216
Hin1II CATG 3 cut(s) 73, 103, 145
Hin6I GCGC 2 cut(s) 162, 244
HinP1I GCGC 2 cut(s) 162, 244
HinfI GANTC 3 cut(s) 19, 28, 391
HpaII CCGG 2 cut(s) 153, 176
Hpy188I TCNGA 2 cut(s) 24, 46
Hpy188III TCNNGA 5 cut(s) 136, 176, 230, 388, 395
Hpy99I CGWCG 1 cut(s) 50
HpyCH4V TGCA 2 cut(s) 73, 336
HpyF10VI GCNNNNNNNGC 2 cut(s) 243, 333
HpyF3I CTNAG 3 cut(s) 21, 184, 393
Hsp92I GRCGYC 1 cut(s) 216
Hsp92II CATG 3 cut(s) 73, 103, 145
HspAI GCGC 2 cut(s) 162, 244
Kpn2I TCCGGA 1 cut(s) 175
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 5 cut(s) 85, 125, 183, 205, 228
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 77
MalI GATC 1 cut(s) 140
MbiI CCGCTC 1 cut(s) 310
MboI GATC 1 cut(s) 138
MboII GAAGA 2 cut(s) 62, 368
MhlI GDGCHC 2 cut(s) 108, 126
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 1 cut(s) 409
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 3 cut(s) 13, 22, 385
MmeI TCCRAC 2 cut(s) 56, 69
Mox20I TGGCCA 1 cut(s) 254
MroI TCCGGA 1 cut(s) 175
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 2 cut(s) 153, 176
MspR9I CCNGG 4 cut(s) 154, 159, 205, 251
MvaI CCWGG 3 cut(s) 159, 205, 251
MwoI GCNNNNNNNGC 2 cut(s) 243, 333
NciI CCSGG 1 cut(s) 154
NcoI CCATGG 1 cut(s) 99
NdeII GATC 1 cut(s) 138
NheI GCTAGC 1 cut(s) 76
NlaIII CATG 3 cut(s) 73, 103, 145
NlaIV GGNNCC 2 cut(s) 209, 328
NmeAIII GCCGAG 1 cut(s) 303
PaeR7I CTCGAG 2 cut(s) 119, 134
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 197
PkrI GCNGC 6 cut(s) 75, 115, 195, 198, 243, 312
PleI GAGTC 3 cut(s) 13, 22, 385
PpsI GAGTC 3 cut(s) 13, 22, 385
PshBI ATTAAT 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 3 cut(s) 157, 203, 249
PspGI CCWGG 3 cut(s) 157, 203, 249
PspN4I GGNNCC 2 cut(s) 209, 328
PspPI GGNCC 3 cut(s) 155, 232, 326
PspXI VCTCGAGB 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 6 cut(s) 74, 114, 194, 197, 242, 311
Sau3AI GATC 1 cut(s) 138
Sau96I GGNCC 3 cut(s) 155, 232, 326
SchI GAGTC 3 cut(s) 13, 22, 385
ScrFI CCNGG 4 cut(s) 154, 159, 205, 251
SduI GDGCHC 2 cut(s) 108, 126
SetI ASST 9 cut(s) 63, 78, 126, 132, 190, 198, 243, 260, 421
SfaNI GCATC 1 cut(s) 55
Sfr274I CTCGAG 2 cut(s) 119, 134
SinI GGWCC 1 cut(s) 232
SlaI CTCGAG 2 cut(s) 119, 134
SmlI CTYRAG 3 cut(s) 107, 119, 134
SmoI CTYRAG 3 cut(s) 107, 119, 134
Sse9I AATT 1 cut(s) 409
SsiI CCGC 4 cut(s) 84, 235, 283, 310
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 152, 157, 203, 249
StyI CCWWGG 1 cut(s) 99
TaqI TCGA 5 cut(s) 8, 63, 120, 126, 135
TasI AATT 1 cut(s) 409
TauI GCSGC 1 cut(s) 313
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseI GCWGC 5 cut(s) 73, 113, 193, 196, 241
TspGWI ACGGA 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 232
VspI ATTAAT 1 cut(s) 432
XagI CCTNNNNNAGG 1 cut(s) 392
XhoI CTCGAG 2 cut(s) 119, 134
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.