MD02G1099900.v1.1

Respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003315
Physical Location & Seq
Reverse (-)
7918841 .. 7925357
6517 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1099900.v1.1.491

Sequence Viewer

Length: 2739 bp
ATGGCGTCGACGGCGTCGTCTAGCGCGAAGCGGTCGAGTTACACCCCTGGGTTTGAGTTACCCTCCTCGCCGTCACCGGTCGGCGGCGCAATGCTGCCGAAGTTTCTCAGAGACCTCAGCCAGGACTTGGTCGAAGTCACATTGGAGCTCGACGACGACGACGACGACGGCTCAATTGTCGTGTGCAGCGTCGCTCCCGCAAACGACGACTTGACGGCGGCGGCGGAGAACGAGATTTTACGGAGGAGTCTGTCCGCGACGTCGAGGAGTGTACGGAGGACGTTCGGGTGGCTGAAATCGGCGTCTTCGAGGACGACGGCGTCAAATTCGGAATCGGTGGTGGAGTTCACTCTGTCGGCTCGCGAGGGGAGGAGGATGAAGGCCAAGCTTCAACGGACCAGATCGAGCGCGCAAAGAGCTCTCAACGGTCTTAGATTCATCAGCAAGACGACTGCCGGAGGAACTGACGCGGCGGAGCAATGGCAGCAAGTGGAGGCGAGGTTCGAGTCGCTCGCTAAGGACGGCTTGCTCGCCAGAGCAGACTTCGGCGAATGTATAGGGATGGGGGACTCGGAGGAGTTTGCGGTGGGGATATTTGATGCATTGGCGAGGCGGCGGCGGCAGAAGATAACCAAAATTACGATAGAGGAGCTTCGTGACTTTTGGTTGCAAATTTCCGACAGCAGCTTCGACGCGCGCCTTCAAATCTTCTTTGACATGGCGGATAGCAATGACGATGGAAGAATCACGAGTGAAGGAGTTCAAGAGCTTATAACGCTCAGTGCTTCTGCAAACAAGCTATCGAAGCTCAAAGAACAAGCTGAAGAGTATGCTTCATTGATAATGGAAGAACTCGATCCTGAAAATTTCGGTTACATCGAGTTATGGCAATTGGAAGCACTGCTTCTACAAAGAGACGTGTACATAAACTACAGCAGACCATTGAGCACAGCAAGTGTAACTTGGAGTCAGAACACCAGTTCCTTGAAGCCCAAGAATCTGGTCCGGAGACTGAGCCGTTCACTTCAGTGTCTCGCACTCGAGAATTGGCGGCGTGGTTGGATTTTACTGTTGTGGGTCGTTGCAATGGCTTCCCTTTTTGTATGGAAATTCTACCGGTACAGAAAAAATAGGGCTACATTTCAGGTCATGCATTATTGCTTGCCAGTGGCCAAAGGGGCTGCAGAGACTCTCAAGCTTAACATGGCTCTCGTCCTCCTACCAGTCTGTCGAAACACGCTGACATGGCTTCGGTCCACAAGAGCCAGGTCCTTCATCCCATTTGATGACAACATTAATTTCCACAAGATTATTGCATTTGCTATAGCAATTGGAATAACCATTCACGCCGGGAACCATTTGGCGTGCGACTTTCCTCGCCTGGTAAACTCTTCGCCGGAAAAATTTGCACCACTATCCTCGGATTTCCATGACAAAAAGCCCACCTATGAATTCCTACTCACCGGAACTGAAGGCGCGACTGGAATTTTGATGGTGATTTTAATAGCTATCTCCTTCACACTAGCAGCTCATAGATTTCGGAGGAACATGGTGAGACTTCCTGCGCCCTTCAACAAGTTGACTGGGTTTAATGCATTCTGGTATTCTCACCATCTGCTAGGTCTTGTATACATTTTGCTAATCATCCATGGAAGCTGCTTGTATTTAGCCCACAACTGGTACGAGAAAACGACATGGATGTACATCTGTGTTCCGTTGTTGCTCTATGTAGCGGAGCGCAGTCTGAGAACATGTAGATCGAAGCATTATTCAGTTAAGATGTTAAAGGTTTCGGTCCTCCCCGGAAATGTGATTAGCTTAATCATGTCCAAACCACCTGGATTTAAGTACAAAAGTGGGCAGTACATATTTCTTCAATGCCCTACAATCTCCCCATTTGAATGGCATCCATTTTCCATTACCTCCGCACCAGGAGACGAGTACCTCAGCGTTCACATCCGAACAGTAGGAGACTGGACTCGAGAATTGAAACGAGCCATAGCGGAGGAAGATGATTCATCTGTAGTTCATCCAGCAAGATTTGCTCAGATGCGAAATATCGATCAGAGAGGCCAACCCAGATTGTTTGTTGACGGTCCATATGGGGCGCCAGCGCAGGACTACCAGCACTACGATGTCTTGCTCCTCGTGGGGCTTGGGATTGGAGCTACCCCTTTTATAAGCATTCTTCGAGATCTTCTTGACAGAGCAAGAACAGCAGAAGAACAAATGGATTCAAATACAGAAACTAGTAGATCAGACGACAGCCTGAGTAGTTTTATGTCTTCAAGTTTGACGCCCGGTGACAAGAAGAAATCTCAGAAGACTACAAATGCTCACTTCTACTGGGTTACCAGAGAGCCTGGATCTTTTGAGTGGTTTAAAGGAGTCATGGACGAGGTTGCAGAAATGGATCACAGAGGTCAGATTGAGCTACACAACTATCTTACAAGTGTCTACGAAGAGGGTGATGCTAGGTCGACTCTAATCACCATGGTCCAAGCTCTCAACCACGCCAAACATGGTGTAGACATCCTATCTGGCACCCGAGTAAGAACTCACTTTGCAAGACCAGACTGGAAACAAGTGTTTACCAAGATAGCTTCCAAGCATCCGTATTCAACAGTCGGGGTATTCTACTGTGGGATGCCAATGTTGGCAAAGGAGCTGAAGGTGCTATCACATGAACTCAGCCAGAAGACAACGACACGGTTTGAATTCCACAAGGAGTATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

913

Amino Acids

103.16

Weight (kDa)

8.69

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 129 - 232 1.3e-38 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 401 - 548 3.4e-10 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 590 - 705 2.8e-33 FAD-binding domain
NAD_binding_6 PF08030 711 - 892 7.7e-49 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 773, 2180
AasI GACNNNNNNGTC 2 cut(s) 16, 319
AatII GACGTC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 2107, 2543
AccB7I CCANNNNNTGG 1 cut(s) 127
AccI GTMKAC 5 cut(s) 8, 1629, 2457, 2480, 2529
AccII CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
AccIII TCCGGA 1 cut(s) 1005
AclWI GGATC 3 cut(s) 851, 2374, 2421
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 8 cut(s) 325, 672, 865, 1109, 1403, 1451, 1485, 2717
AcuI CTGAAG 4 cut(s) 843, 1010, 1491, 2690
AcyI GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
AfaI GTAC 8 cut(s) 273, 923, 1121, 1682, 1703, 1850, 1865, 1943
AfiI CCNNNNNNNGG 4 cut(s) 83, 121, 127, 1677
AflIII ACRYGT 2 cut(s) 918, 1751
AgeI ACCGGT 2 cut(s) 76, 1116
AhlI ACTAGT 1 cut(s) 2249
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 7 cut(s) 46, 120, 1265, 1380, 1837, 1930, 2362
AleI CACNNNNGTG 1 cut(s) 1027
AloI GAACNNNNNNTCC 4 cut(s) 485, 517, 965, 997
Alw21I GWGCWC 3 cut(s) 150, 421, 950
Alw26I GTCTC 8 cut(s) 105, 909, 1003, 1037, 1181, 1549, 1929, 1965
AlwI GGATC 3 cut(s) 851, 2374, 2421
Ama87I CYCGRG 3 cut(s) 1040, 1980, 2547
Aor13HI TCCGGA 1 cut(s) 1005
AoxI GGCC 3 cut(s) 381, 1170, 2071
ApeKI GCWGC 7 cut(s) 94, 186, 484, 684, 1181, 1526, 1656
ApoI RAATTY 8 cut(s) 325, 672, 865, 1109, 1403, 1451, 1485, 2717
ArsI GACNNNNNNTTYG 4 cut(s) 671, 703, 1471, 1503
AseI ATTAAT 1 cut(s) 1296
AsiGI ACCGGT 2 cut(s) 76, 1116
Asp700I GAANNNNTTC 1 cut(s) 759
AspS9I GGNCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
AsuC2I CCSGG 3 cut(s) 1351, 1803, 2301
AsuHPI GGTGA 8 cut(s) 66, 1453, 1507, 1564, 1601, 2315, 2480, 2482
AvaI CYCGRG 3 cut(s) 1040, 1980, 2547
AvaII GGWCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
BalI TGGCCA 1 cut(s) 1172
BanI GGYRCC 2 cut(s) 2107, 2543
BanII GRGCYC 2 cut(s) 150, 421
BauI CACGAG 2 cut(s) 748, 2147
BbsI GAAGAC 4 cut(s) 297, 2277, 2330, 2705
Bbv12I GWGCWC 3 cut(s) 150, 421, 950
BbvCI CCTCAGC 2 cut(s) 116, 1946
BbvI GCAGC 7 cut(s) 81, 198, 496, 696, 1168, 1538, 1643
BccI CCATC 4 cut(s) 556, 731, 1486, 1620
BceAI ACGGC 7 cut(s) 27, 55, 184, 231, 333, 538, 1002
BciT130I CCWGG 7 cut(s) 48, 122, 1267, 1382, 1839, 1932, 2364
BcnI CCSGG 3 cut(s) 1351, 1803, 2301
BcoDI GTCTC 8 cut(s) 105, 909, 1003, 1037, 1181, 1549, 1929, 1965
BcuI ACTAGT 1 cut(s) 2249
BfaI CTAG 5 cut(s) 21, 1523, 1619, 2250, 2475
BfmI CTRYAG 4 cut(s) 931, 1182, 1323, 2022
BfoI RGCGCY 1 cut(s) 2111
BglI GCCNNNNNGGC 1 cut(s) 1178
BglII AGATCT 1 cut(s) 2194
Bme18I GGWCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
BmeT110I CYCGRG 3 cut(s) 1040, 1980, 2547
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
BmiI GGNNCC 3 cut(s) 1355, 2109, 2545
BmrI ACTGGG 2 cut(s) 1593, 2356
BmsI GCATC 6 cut(s) 589, 1915, 2040, 2461, 2620, 2637
BmuI ACTGGG 2 cut(s) 1593, 2356
BpiI GAAGAC 4 cut(s) 297, 2277, 2330, 2705
BplI GAGNNNNNCTC 2 cut(s) 47, 79
Bpu10I CCTNAGC 3 cut(s) 116, 516, 1946
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 3 cut(s) 1351, 1803, 2301
Bsa29I ATCGAT 1 cut(s) 2061
BsaBI GATNNNNATC 1 cut(s) 1703
BsaHI GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 6 cut(s) 46, 47, 1419, 1648, 1801, 2493
BsaWI WCCGGW 4 cut(s) 76, 1005, 1116, 1463
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 121, 127, 1677
Bse118I RCCGGY 2 cut(s) 76, 1116
Bse1I ACTGG 9 cut(s) 978, 1166, 1223, 1486, 1588, 1682, 1979, 2351, 2582
Bse3DI GCAATG 4 cut(s) 96, 485, 736, 1092
Bse8I GATNNNNATC 1 cut(s) 1703
BseAI TCCGGA 1 cut(s) 1005
BseBI CCWGG 7 cut(s) 48, 122, 1267, 1382, 1839, 1932, 2364
BseCI ATCGAT 1 cut(s) 2061
BseDI CCNNGG 6 cut(s) 46, 47, 1419, 1648, 1801, 2493
BseJI GATNNNNATC 1 cut(s) 1703
BseLI CCNNNNNNNGG 4 cut(s) 83, 121, 127, 1677
BseMI GCAATG 4 cut(s) 96, 485, 736, 1092
BseNI ACTGG 9 cut(s) 978, 1166, 1223, 1486, 1588, 1682, 1979, 2351, 2582
BsePI GCGCGC 2 cut(s) 408, 695
BseRI GAGGAG 7 cut(s) 55, 259, 280, 385, 590, 662, 2135
BseXI GCAGC 7 cut(s) 81, 198, 496, 696, 1168, 1538, 1643
BsgI GTGCAG 1 cut(s) 205
Bsh1236I CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
Bsh1285I CGRYCG 2 cut(s) 35, 81
BshFI GGCC 3 cut(s) 383, 1172, 2073
BshNI GGYRCC 2 cut(s) 2107, 2543
BshTI ACCGGT 2 cut(s) 76, 1116
BshVI ATCGAT 1 cut(s) 2061
BsiEI CGRYCG 2 cut(s) 35, 81
BsiHKAI GWGCWC 3 cut(s) 150, 421, 950
BsiHKCI CYCGRG 3 cut(s) 1040, 1980, 2547
BsiSI CCGG 9 cut(s) 77, 456, 1006, 1117, 1350, 1397, 1464, 1803, 2301
BslFI GGGAC 1 cut(s) 581
BslI CCNNNNNNNGG 4 cut(s) 83, 121, 127, 1677
BsmAI GTCTC 8 cut(s) 105, 909, 1003, 1037, 1181, 1549, 1929, 1965
BsmBI CGTCTC 2 cut(s) 909, 1929
BsmFI GGGAC 1 cut(s) 581
BsmI GAATGC 2 cut(s) 1595, 2184
BsnI GGCC 3 cut(s) 383, 1172, 2073
Bso31I GGTCTC 1 cut(s) 105
BsoBI CYCGRG 3 cut(s) 1040, 1980, 2547
Bsp1286I GDGCHC 3 cut(s) 150, 421, 950
Bsp13I TCCGGA 1 cut(s) 1005
Bsp1407I TGTACA 2 cut(s) 921, 1701
Bsp143I GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
Bsp19I CCATGG 2 cut(s) 1648, 2493
Bsp68I TCGCGA 1 cut(s) 363
BspANI GGCC 3 cut(s) 383, 1172, 2073
BspDI ATCGAT 1 cut(s) 2061
BspEI TCCGGA 1 cut(s) 1005
BspFNI CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
BspLI GGNNCC 3 cut(s) 1355, 2109, 2545
BspMAI CTGCAG 1 cut(s) 1186
BspPI GGATC 3 cut(s) 851, 2374, 2421
BspT107I GGYRCC 2 cut(s) 2107, 2543
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 4 cut(s) 96, 485, 736, 1092
BsrFI RCCGGY 2 cut(s) 76, 1116
BsrGI TGTACA 2 cut(s) 921, 1701
BsrI ACTGG 9 cut(s) 978, 1166, 1223, 1486, 1588, 1682, 1979, 2351, 2582
BssAI RCCGGY 2 cut(s) 76, 1116
BssECI CCNNGG 6 cut(s) 46, 47, 1419, 1648, 1801, 2493
BssHII GCGCGC 2 cut(s) 408, 695
BssMI GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
BssNAI GTATAC 1 cut(s) 1630
BssNI GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
BssSI CACGAG 2 cut(s) 748, 2147
BssT1I CCWWGG 2 cut(s) 1648, 2493
Bst1107I GTATAC 1 cut(s) 1630
Bst2BI CACGAG 2 cut(s) 748, 2147
Bst2UI CCWGG 7 cut(s) 48, 122, 1267, 1382, 1839, 1932, 2364
Bst4CI ACNGT 7 cut(s) 428, 1071, 1966, 2096, 2626, 2642, 2712
Bst6I CTCTTC 3 cut(s) 819, 1396, 2457
BstACI GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
BstAPI GCANNNNNTGC 1 cut(s) 2042
BstAUI TGTACA 2 cut(s) 921, 1701
BstC8I GCNNGC 9 cut(s) 361, 410, 513, 527, 531, 697, 1163, 1366, 2112
BstDSI CCRYGG 2 cut(s) 1648, 2493
BstEII GGTNACC 1 cut(s) 2350
BstENI CCTNNNNNAGG 1 cut(s) 119
BstFNI CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
BstH2I RGCGCY 1 cut(s) 2111
BstKTI GATC 8 cut(s) 404, 859, 1760, 2065, 2197, 2258, 2369, 2416
BstMAI GTCTC 8 cut(s) 105, 909, 1003, 1037, 1181, 1549, 1929, 1965
BstMBI GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
BstMCI CGRYCG 2 cut(s) 35, 81
BstNI CCWGG 7 cut(s) 48, 122, 1267, 1382, 1839, 1932, 2364
BstNSI RCATGY 1 cut(s) 1755
BstPI GGTNACC 1 cut(s) 2350
BstSFI CTRYAG 4 cut(s) 931, 1182, 1323, 2022
BstUI CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
BstV1I GCAGC 7 cut(s) 81, 198, 496, 696, 1168, 1538, 1643
BstV2I GAAGAC 4 cut(s) 297, 2277, 2330, 2705
BstX2I RGATCY 2 cut(s) 2194, 2366
BstXI CCANNNNNNTGG 2 cut(s) 1000, 1902
BstYI RGATCY 2 cut(s) 2194, 2366
BstZ17I GTATAC 1 cut(s) 1630
Bsu15I ATCGAT 1 cut(s) 2061
BsuRI GGCC 3 cut(s) 383, 1172, 2073
BsuTUI ATCGAT 1 cut(s) 2061
BtgI CCRYGG 2 cut(s) 1648, 2493
BtrI CACGTC 1 cut(s) 919
BtsI GCAGTG 1 cut(s) 899
BtsIMutI CAGTG 4 cut(s) 787, 899, 1034, 1173
BtuMI TCGCGA 1 cut(s) 363
Cac8I GCNNGC 9 cut(s) 361, 410, 513, 527, 531, 697, 1163, 1366, 2112
Cfr10I RCCGGY 2 cut(s) 76, 1116
Cfr13I GGNCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
ClaI ATCGAT 1 cut(s) 2061
CseI GACGC 7 cut(s) 3, 178, 291, 309, 476, 701, 2305
Csp6I GTAC 8 cut(s) 272, 922, 1120, 1681, 1702, 1849, 1864, 1942
CspAI ACCGGT 2 cut(s) 76, 1116
CviQI GTAC 8 cut(s) 272, 922, 1120, 1681, 1702, 1849, 1864, 1942
DinI GGCGCC 1 cut(s) 2109
DpnI GATC 8 cut(s) 403, 858, 1759, 2064, 2196, 2257, 2368, 2415
DpnII GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
DraI TTTAAA 1 cut(s) 2383
DrdI GACNNNNNNGTC 2 cut(s) 16, 319
DseDI GACNNNNNNGTC 2 cut(s) 16, 319
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 3 cut(s) 819, 1396, 2457
EarI CTCTTC 3 cut(s) 819, 1396, 2457
EciI GGCGGA 3 cut(s) 239, 488, 737
Ecl136II GAGCTC 2 cut(s) 148, 419
Eco130I CCWWGG 2 cut(s) 1648, 2493
Eco24I GRGCYC 2 cut(s) 150, 421
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
Eco53kI GAGCTC 2 cut(s) 148, 419
Eco57I CTGAAG 4 cut(s) 843, 1010, 1491, 2690
Eco88I CYCGRG 3 cut(s) 1040, 1980, 2547
Eco91I GGTNACC 1 cut(s) 2350
EcoICRI GAGCTC 2 cut(s) 148, 419
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 1269
EcoO65I GGTNACC 1 cut(s) 2350
EcoRI GAATTC 2 cut(s) 1451, 2717
EcoRII CCWGG 7 cut(s) 46, 120, 1265, 1380, 1837, 1930, 2362
EcoT14I CCWWGG 2 cut(s) 1648, 2493
EcoT22I ATGCAT 3 cut(s) 604, 1155, 1597
EcoT38I GRGCYC 2 cut(s) 150, 421
EgeI GGCGCC 1 cut(s) 2109
EheI GGCGCC 1 cut(s) 2109
ErhI CCWWGG 2 cut(s) 1648, 2493
Esp3I CGTCTC 2 cut(s) 909, 1929
FalI AAGNNNNNCTT 6 cut(s) 509, 541, 888, 920, 946, 978
FaqI GGGAC 1 cut(s) 581
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 2101
FblI GTMKAC 5 cut(s) 8, 1629, 2457, 2480, 2529
FriOI GRGCYC 2 cut(s) 150, 421
FspBI CTAG 5 cut(s) 21, 1523, 1619, 2250, 2475
HaeII RGCGCY 1 cut(s) 2111
HaeIII GGCC 3 cut(s) 383, 1172, 2073
HapII CCGG 9 cut(s) 77, 456, 1006, 1117, 1350, 1397, 1464, 1803, 2301
HgaI GACGC 7 cut(s) 3, 178, 291, 309, 476, 701, 2305
Hin1I GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
HincII GTYRAC 4 cut(s) 9, 1581, 2092, 2481
HindII GTYRAC 4 cut(s) 9, 1581, 2092, 2481
HindIII AAGCTT 2 cut(s) 386, 1196
HpaII CCGG 9 cut(s) 77, 456, 1006, 1117, 1350, 1397, 1464, 1803, 2301
HphI GGTGA 8 cut(s) 66, 1453, 1507, 1564, 1601, 2315, 2480, 2482
HpyAV CCTTC 8 cut(s) 373, 710, 749, 1282, 1466, 1525, 1579, 2665
HpyCH4III ACNGT 7 cut(s) 428, 1071, 1966, 2096, 2626, 2642, 2712
HpyCH4IV ACGT 3 cut(s) 260, 281, 918
HpySE526I ACGT 3 cut(s) 260, 281, 918
Hsp92I GRCGYC 7 cut(s) 5, 14, 260, 302, 320, 2108, 2297
KasI GGCGCC 1 cut(s) 2107
Kpn2I TCCGGA 1 cut(s) 1005
Kzo9I GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
LmnI GCTCC 8 cut(s) 145, 199, 475, 649, 1735, 2148, 2165, 2665
Lsp1109I GCAGC 7 cut(s) 81, 198, 496, 696, 1168, 1538, 1643
LweI GCATC 6 cut(s) 589, 1915, 2040, 2461, 2620, 2637
MaeI CTAG 5 cut(s) 21, 1523, 1619, 2250, 2475
MaeII ACGT 3 cut(s) 260, 281, 918
MaeIII GTNAC 9 cut(s) 38, 57, 72, 136, 656, 872, 958, 2303, 2350
MalI GATC 8 cut(s) 403, 858, 1759, 2064, 2196, 2257, 2368, 2415
MboI GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
MfeI CAATTG 3 cut(s) 174, 890, 1329
MflI RGATCY 2 cut(s) 2194, 2366
MhlI GDGCHC 3 cut(s) 150, 421, 950
MlsI TGGCCA 1 cut(s) 1172
MluNI TGGCCA 1 cut(s) 1172
Mly113I GGCGCC 1 cut(s) 2108
MlyI GAGTC 8 cut(s) 256, 515, 563, 976, 1183, 1972, 2397, 2476
MmeI TCCRAC 2 cut(s) 702, 1040
Mox20I TGGCCA 1 cut(s) 1172
Mph1103I ATGCAT 3 cut(s) 604, 1155, 1597
MroI TCCGGA 1 cut(s) 1005
MroXI GAANNNNTTC 1 cut(s) 759
MscI TGGCCA 1 cut(s) 1172
MseI TTAA 9 cut(s) 1200, 1296, 1502, 1590, 1776, 1784, 1820, 1845, 2382
MslI CAYNNNNRTG 3 cut(s) 1027, 1900, 2133
Msp20I TGGCCA 1 cut(s) 1172
MspI CCGG 9 cut(s) 77, 456, 1006, 1117, 1350, 1397, 1464, 1803, 2301
MunI CAATTG 3 cut(s) 174, 890, 1329
Mva1269I GAATGC 2 cut(s) 1595, 2184
MvaI CCWGG 7 cut(s) 48, 122, 1267, 1382, 1839, 1932, 2364
MvnI CGCG 8 cut(s) 26, 257, 363, 410, 470, 695, 697, 1478
NarI GGCGCC 1 cut(s) 2108
NciI CCSGG 3 cut(s) 1351, 1803, 2301
NcoI CCATGG 2 cut(s) 1648, 2493
NdeI CATATG 1 cut(s) 2101
NdeII GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
NlaIV GGNNCC 3 cut(s) 1355, 2109, 2545
NmuCI GTSAC 4 cut(s) 72, 136, 656, 2303
NruI TCGCGA 1 cut(s) 363
NsiI ATGCAT 3 cut(s) 604, 1155, 1597
NspI RCATGY 1 cut(s) 1755
OliI CACNNNNGTG 1 cut(s) 1027
PaeR7I CTCGAG 2 cut(s) 1040, 1980
PasI CCCWGGG 1 cut(s) 47
PauI GCGCGC 2 cut(s) 408, 695
PciI ACATGT 1 cut(s) 1751
PcsI WCGNNNNNNNCGW 8 cut(s) 14, 23, 156, 159, 162, 186, 305, 876
PctI GAATGC 2 cut(s) 1595, 2184
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 6 cut(s) 332, 435, 744, 997, 2015, 2234
PflFI GACNNNGTC 3 cut(s) 13, 128, 319
PflMI CCANNNNNTGG 1 cut(s) 127
PinAI ACCGGT 2 cut(s) 76, 1116
PleI GAGTC 8 cut(s) 255, 514, 563, 975, 1183, 1972, 2396, 2476
PluTI GGCGCC 1 cut(s) 2111
PpsI GAGTC 8 cut(s) 255, 514, 563, 975, 1183, 1972, 2396, 2476
PpuMI RGGWCCY 1 cut(s) 1269
PscI ACATGT 1 cut(s) 1751
PshBI ATTAAT 1 cut(s) 1296
PsiI TTATAA 2 cut(s) 773, 2180
Psp124BI GAGCTC 2 cut(s) 150, 421
Psp5II RGGWCCY 1 cut(s) 1269
Psp6I CCWGG 7 cut(s) 46, 120, 1265, 1380, 1837, 1930, 2362
PspEI GGTNACC 1 cut(s) 2350
PspGI CCWGG 7 cut(s) 46, 120, 1265, 1380, 1837, 1930, 2362
PspN4I GGNNCC 3 cut(s) 1355, 2109, 2545
PspPI GGNCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
PspPPI RGGWCCY 1 cut(s) 1269
PstI CTGCAG 1 cut(s) 1186
PsuI RGATCY 2 cut(s) 2194, 2366
PsyI GACNNNGTC 3 cut(s) 13, 128, 319
PteI GCGCGC 2 cut(s) 408, 695
RruI TCGCGA 1 cut(s) 363
RsaI GTAC 8 cut(s) 273, 923, 1121, 1682, 1703, 1850, 1865, 1943
RsaNI GTAC 8 cut(s) 272, 922, 1120, 1681, 1702, 1849, 1864, 1942
RseI CAYNNNNRTG 3 cut(s) 1027, 1900, 2133
SacI GAGCTC 2 cut(s) 150, 421
SalI GTCGAC 2 cut(s) 7, 2479
SaqAI TTAA 9 cut(s) 1200, 1296, 1502, 1590, 1776, 1784, 1820, 1845, 2382
Sau3AI GATC 8 cut(s) 401, 856, 1757, 2062, 2194, 2255, 2366, 2413
Sau96I GGNCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
SchI GAGTC 8 cut(s) 256, 515, 563, 976, 1183, 1972, 2397, 2476
SduI GDGCHC 3 cut(s) 150, 421, 950
SfaNI GCATC 6 cut(s) 589, 1915, 2040, 2461, 2620, 2637
SfcI CTRYAG 4 cut(s) 931, 1182, 1323, 2022
SfoI GGCGCC 1 cut(s) 2109
Sfr274I CTCGAG 2 cut(s) 1040, 1980
SgrDI CGTCGACG 1 cut(s) 7
SinI GGWCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
SlaI CTCGAG 2 cut(s) 1040, 1980
SmiMI CAYNNNNRTG 3 cut(s) 1027, 1900, 2133
SmlI CTYRAG 3 cut(s) 1040, 1193, 1980
SmoI CTYRAG 3 cut(s) 1040, 1193, 1980
SpeI ACTAGT 1 cut(s) 2249
SspDI GGCGCC 1 cut(s) 2107
SspMI CTAG 5 cut(s) 21, 1523, 1619, 2250, 2475
SstI GAGCTC 2 cut(s) 150, 421
StyI CCWWGG 2 cut(s) 1648, 2493
TaaI ACNGT 7 cut(s) 428, 1071, 1966, 2096, 2626, 2642, 2712
TaiI ACGT 3 cut(s) 263, 284, 921
TaqII GACCGA 2 cut(s) 1242, 1783
TatI WGTACW 4 cut(s) 921, 1701, 1848, 1863
TauI GCSGC 8 cut(s) 87, 221, 224, 473, 616, 619, 622, 1054
TfiI GAWTC 6 cut(s) 332, 435, 744, 997, 2015, 2234
Tru1I TTAA 9 cut(s) 1200, 1296, 1502, 1590, 1776, 1784, 1820, 1845, 2382
Tru9I TTAA 9 cut(s) 1200, 1296, 1502, 1590, 1776, 1784, 1820, 1845, 2382
TscAI CASTG 4 cut(s) 787, 906, 1034, 1173
TseFI GTSAC 4 cut(s) 72, 136, 656, 2303
TseI GCWGC 7 cut(s) 94, 186, 484, 684, 1181, 1526, 1656
Tsp45I GTSAC 4 cut(s) 72, 136, 656, 2303
TspDTI ATGAA 8 cut(s) 392, 427, 825, 1264, 1464, 2007, 2018, 2700
TspGWI ACGGA 5 cut(s) 256, 289, 409, 1704, 2604
TspRI CASTG 4 cut(s) 787, 906, 1034, 1173
Tth111I GACNNNGTC 3 cut(s) 13, 128, 319
Van91I CCANNNNNTGG 1 cut(s) 127
VpaK11BI GGWCC 7 cut(s) 396, 1003, 1254, 1269, 1795, 2096, 2497
VspI ATTAAT 1 cut(s) 1296
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 8 cut(s) 325, 672, 865, 1109, 1403, 1451, 1485, 2717
XceI RCATGY 1 cut(s) 1755
XcmI CCANNNNNNNNNTGG 1 cut(s) 2519
XhoI CTCGAG 2 cut(s) 1040, 1980
XmiI GTMKAC 5 cut(s) 8, 1629, 2457, 2480, 2529
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 5 cut(s) 21, 1523, 1619, 2250, 2475
ZraI GACGTC 1 cut(s) 261
Zsp2I ATGCAT 3 cut(s) 604, 1155, 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.