MD12G1213800.v1.1

Respiratory burst

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003315
Physical Location & Seq
Reverse (-)
29205348 .. 29206110
763 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213800.v1.1.491

Sequence Viewer

Length: 345 bp
ATGAATGAGGTGGCCGGAACTGATATAGATGGTGTAATTGTGCTCCGTAACTACTGCACAAGTGTGTACGAAGGAGATGCTCGATCGGCTCTTACCACAATGCTTCAATCTCTTAATCATGCCAAAAACGGCATCGACGTAGTTTCGGGTACGAGGGTTAAGACTCATTTTCCTAGATCGAATTGGCGTGACGTTTTTAAGCGTGTCGCAATTAAGCACACTGATCAACAAGTTGGTGTATTTTACAGTGGTGCACATGGATCAGTAGGGGAGTTAAAGAGGTTAGCTCAGGATTTTTCCAGAAAAACCGAGACCAAGTTTGATTTCCATAAAGAAAACTTTTAA

Protein Analysis

115

Amino Acids

12.85

Weight (kDa)

9.17

Isoelectric Point (pI)

30.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_6 PF08030 1 - 96 1.4e-16 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 268
AcoI YGGCCR 1 cut(s) 12
AfaI GTAC 2 cut(s) 68, 151
AgsI TTSAA 1 cut(s) 107
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 1 cut(s) 287
AluI AGCT 1 cut(s) 287
Alw21I GWGCWC 2 cut(s) 45, 256
Alw26I GTCTC 1 cut(s) 305
Alw44I GTGCAC 1 cut(s) 252
AlwI GGATC 1 cut(s) 268
AoxI GGCC 1 cut(s) 12
ApaLI GTGCAC 1 cut(s) 252
BaeGI GKGCMC 1 cut(s) 256
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
Bbv12I GWGCWC 2 cut(s) 45, 256
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 59, 93
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 174
BmsI GCATC 2 cut(s) 67, 141
BplI GAGNNNNNCTC 2 cut(s) 271, 303
Bpu10I CCTNAGC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 305
BseMII CTCAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 14
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 45, 256
BsiSI CCGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 305
BsnI GGCC 1 cut(s) 14
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 45, 256
Bsp143I GATC 4 cut(s) 83, 176, 223, 260
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 301
BspPI GGATC 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 305
BssMI GATC 4 cut(s) 83, 176, 223, 260
Bst4CI ACNGT 1 cut(s) 248
BstDEI CTNAG 1 cut(s) 288
BstKTI GATC 4 cut(s) 86, 179, 226, 263
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 4 cut(s) 83, 176, 223, 260
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstSLI GKGCMC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 14
BtsIMutI CAGTG 2 cut(s) 219, 253
Csp6I GTAC 2 cut(s) 67, 150
CviAII CATG 2 cut(s) 119, 257
CviJI RGCY 3 cut(s) 14, 89, 287
CviKI_1 RGCY 3 cut(s) 14, 89, 287
CviQI GTAC 2 cut(s) 67, 150
DdeI CTNAG 1 cut(s) 288
DpnI GATC 4 cut(s) 85, 178, 225, 262
DpnII GATC 4 cut(s) 83, 176, 223, 260
EaeI YGGCCR 1 cut(s) 12
Eco31I GGTCTC 1 cut(s) 305
FaeI CATG 2 cut(s) 122, 260
FaiI YATR 4 cut(s) 26, 120, 258, 330
FatI CATG 2 cut(s) 118, 256
FbaI TGATCA 1 cut(s) 223
FspBI CTAG 1 cut(s) 174
HaeIII GGCC 1 cut(s) 14
HapII CCGG 1 cut(s) 15
Hin1II CATG 2 cut(s) 122, 260
HinfI GANTC 1 cut(s) 163
HpaII CCGG 1 cut(s) 15
Hpy166II GTNNAC 2 cut(s) 67, 254
Hpy188III TCNNGA 2 cut(s) 290, 300
Hpy8I GTNNAC 2 cut(s) 67, 254
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 2 cut(s) 138, 192
HpyCH4V TGCA 2 cut(s) 57, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 2 cut(s) 138, 192
Hsp92II CATG 2 cut(s) 122, 260
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 4 cut(s) 83, 176, 223, 260
LmnI GCTCC 1 cut(s) 48
LpnPI CCDG 3 cut(s) 28, 275, 313
LweI GCATC 2 cut(s) 67, 141
MaeI CTAG 1 cut(s) 174
MaeII ACGT 2 cut(s) 138, 192
MaeIII GTNAC 2 cut(s) 47, 188
MalI GATC 4 cut(s) 85, 178, 225, 262
MboI GATC 4 cut(s) 83, 176, 223, 260
MhlI GDGCHC 2 cut(s) 45, 256
MluCI AATT 3 cut(s) 36, 181, 210
MlyI GAGTC 1 cut(s) 157
MnlI CCTC 2 cut(s) 147, 273
MseI TTAA 6 cut(s) 114, 159, 198, 213, 275, 343
MslI CAYNNNNRTG 1 cut(s) 62
MspI CCGG 1 cut(s) 15
MwoI GCNNNNNNNGC 1 cut(s) 86
NdeII GATC 4 cut(s) 83, 176, 223, 260
NlaIII CATG 2 cut(s) 122, 260
NmuCI GTSAC 1 cut(s) 188
OliI CACNNNNGTG 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 135
Ple19I CGATCG 1 cut(s) 86
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PvuI CGATCG 1 cut(s) 86
RsaI GTAC 2 cut(s) 68, 151
RsaNI GTAC 2 cut(s) 67, 150
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 6 cut(s) 114, 159, 198, 213, 275, 343
Sau3AI GATC 4 cut(s) 83, 176, 223, 260
SchI GAGTC 1 cut(s) 157
SduI GDGCHC 2 cut(s) 45, 256
SetI ASST 5 cut(s) 12, 141, 195, 284, 289
SfaNI GCATC 2 cut(s) 67, 141
SmiMI CAYNNNNRTG 1 cut(s) 62
Sse9I AATT 3 cut(s) 36, 181, 210
SspMI CTAG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 2 cut(s) 141, 195
TaqI TCGA 3 cut(s) 82, 135, 179
TasI AATT 3 cut(s) 36, 181, 210
Tru1I TTAA 6 cut(s) 114, 159, 198, 213, 275, 343
Tru9I TTAA 6 cut(s) 114, 159, 198, 213, 275, 343
TscAI CASTG 2 cut(s) 226, 253
TseFI GTSAC 1 cut(s) 188
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 2 cut(s) 226, 253
VneI GTGCAC 1 cut(s) 252
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.