Rroxscaffold_2G00146050

Respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003315
Physical Location & Seq
Forward (+)
83904870 .. 83909502
4633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146050.1

Sequence Viewer

Length: 2508 bp
ATGCTAGGAAGGAGCCTCTCCGCTACGTCACGGAGCATTCGCCGCACATTCGGGTGGCTGATCAGATCGTCGTCGTCGAGGACCGCCGAGTCTTCCAACTCCGGCGAACTCGCGGTTCCGCCACGAGAGGCGAGGAGGCTGAAGGCGAAGCTTCAACGGACAAGATCGAGCGCGCAAAGAGCTCTCAACGGCCTGAGGTTCATCAGCAAGACCACCGGACCTAGCGACACCGAGGAGCTGTGGAAGCAAGTGGAGGCCAGGTTCGAGTCGCTGTCCGTCCACGGATTGCTCGCCAGAGGAGACTTTGGTCGATGCATAGGGATGGCGGACTCGGAGGAGTTTGCGGTGGGGATATTTGACACATTGGCGAGGAGGCGGCGGCAGAAAATTACGAAGATCACGAAGGAAGAACTTCATGATTTTTGGATACAAATCTCGGACCAGAGCTTTGATGCGCGCCTTCAAATTTTCTTCGACATGGCAGATAGCAATGAGGATGGAAGAATCACAAGGGAGGGAGTGCAAGAGCTTATAATGCTGAGTGCTTCTGCAAACAAGCTATCAAAGCTGAAAGAACAAGCTGAAGAATATGCTTCATTGATAATGGAAGAACTAGACCCCGAAAACTTTGGTTTTATTGAGTTATGGCAATTGGAAGCACTGCTTTTACAAAGAGACGTATACATAAACTACAGCAGACCATTGAGCGCGGTAAGTGTAGGTTGGAGTCAGAACCTGAGTTCCTTCAGGCCCAAGAATCTACTTCGGAGACTAAATCGTTCACTTCAGTGTCTCATACTAGACAATTGGCAGCGTGGTTGGATTTTATTATTGTGGATTGTTGCAATGGCTTCACTTTTTGCCTGGAAATTCTACCAATACAGAAACAAAGCAGCATTTGAAGTCATCGGCTATTGCTTGCCAGTAGCCAAAGGAGCTGCAGAAACTTTGAAGCTTAACATGGCCCTCATCCTTTTACCTGTTTGTCGTAACACACTGACATGGCTTCGATCAACACGAGCTAGGTCCTTCATTCCCTTTGATGACAACATTAATTTCCACAAGATGATTGCATTTGCTATAGCAATTGGAATAATTGTTCATGCTGGGAACCATGTGGCATGTGACTTTCCTCGTCTAGTAAACTCATCTGCGGAAGAATTTGAGCCAATATCCTCTGATTTCCATGGCAAAAAACCGACATACAAATTCTTATTCACTGGACCAGAAGGCTTGACTGGAATTCTGATGGTGATTTTTATGGCCATCTCATTCACGCTAGCAGCGAGACACTTTCGAAGGAACATGGTGAGGCTCCCTGCCCCCCTGAACAAACTGACTGGGTTTAATGCGTTCTGGTATTCTCATCATCTGCTAGGTCTGGTCTACGTTTTGCTACTCATCCATGGAAGCTGTTTATACTTTGTCCACGAGTGGTATCAGAAAACGACATGGATGTACATCTCTGTTCCATTTCTGCTGTATGTTGTAGAGCGCAGTATCCGAACATGTAGATCAAAACACTATTCAGTTAAGATACTAAAGGCTACAATCTTACCCGGAAATGTTATCAGCTTAATCATGTCCAAGCCACCTGGATTTAAGTACAGAAGTGGGCAATACATATTTCTACAATGCCCAACAATCTCCCCATTTGAATGGCACCCATTTTCCATCACCTCAGCACCTGGGGATGAGCATCTCAGTGTTCATATCCGAACAGTTGGAGACTGGACGCAAGAATTGAAGCGAGCCATTTCTGAGGGAGATCATTCATTATCTTTAGTTCATCAAACTAGTGTTGCTCAGCCACAAGATATGGATCAAATAAGACTATGCAGCCAACCCAAATTGTTTTTTGATGGTCCATATGGGGCACCGGCACAGGACTACCGCAACTATGATGTCCTACTCCTTGTGGGTCTTGGCATTGGAGCTACCCCTTTTGTAAGCATCCTTAAAGATCTTCTTAACAGCACAAGAACAGCAGAAGAACAACTGGATTCAAATACAGAAACTAGTACACCATCAGGCAGCCTAAATAGTTTTACATCATCATGTTTGACACCCAATGACAAGAAGAAATCACAGAGGACTACAAATGCTCATTTCTATTGGGTTACCAGAGAACCTGGCTCTTTCGAGTGGTTTAAAGGAGTCATGGATGAAGTGGCAGAAATGGATCACAAAGGTCAGATTGAACTTCACAACTACCTTACGAGTGTCTACGAAGAGGGTGATGCAAGGTCAACTCTAATCACCATGGTCCAAGCTCTCAACCATGCCAAACATGGAGTTGACATCCTATCTGGCACCAGAGTAATGACTCATTTTGCAAGACCTAATTGGAAAGAAGTGTTTACCAAGATAGCTTCCAAGCATCCATTTTCGACAGTAGGGGTATTCTACTGTGGGATGCCAATGTTAGCAAAGGAGCTGAAGATGCTATCACTTGAGCTCAGTCACAAGACATCGACACGCTTTGAATTCCACAAGGAGTACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

835

Amino Acids

95.2

Weight (kDa)

9.16

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 50 - 152 1.8e-38 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 320 - 466 6.6e-11 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 509 - 628 9.2e-31 FAD-binding domain
NAD_binding_6 PF08030 634 - 816 1.1e-48 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AasI GACNNNNNNGTC 1 cut(s) 88
AccB1I GGYRCC 3 cut(s) 1662, 1876, 2312
AccI GTMKAC 3 cut(s) 681, 1386, 2226
AccII CGCG 4 cut(s) 113, 173, 457, 710
AclWI GGATC 2 cut(s) 1830, 2190
AcoI YGGCCR 1 cut(s) 1263
AcsI RAATTY 6 cut(s) 465, 869, 1160, 1208, 1242, 2486
AcuI CTGAAG 5 cut(s) 161, 603, 730, 770, 2459
AfaI GTAC 4 cut(s) 1460, 1607, 2023, 2501
AflIII ACRYGT 1 cut(s) 1508
AgsI TTSAA 9 cut(s) 155, 464, 902, 952, 1658, 1747, 2007, 2201, 2486
AhlI ACTAGT 2 cut(s) 1796, 2018
AjnI CCWGG 5 cut(s) 257, 863, 1594, 1687, 2131
AleI CACNNNNGTG 1 cut(s) 787
AloI GAACNNNNNNTCC 6 cut(s) 245, 277, 725, 757, 1083, 1115
Alw21I GWGCWC 2 cut(s) 184, 2460
Alw26I GTCTC 6 cut(s) 294, 669, 763, 797, 1282, 1722
AlwI GGATC 2 cut(s) 1830, 2190
AlwNI CAGNNNCTG 2 cut(s) 736, 1688
AoxI GGCC 5 cut(s) 190, 255, 749, 963, 1263
ApeKI GCWGC 6 cut(s) 811, 893, 938, 1283, 1839, 2034
ApoI RAATTY 6 cut(s) 465, 869, 1160, 1208, 1242, 2486
ArsI GACNNNNNNTTYG 2 cut(s) 431, 463
AseI ATTAAT 1 cut(s) 1053
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 6 cut(s) 173, 175, 457, 459, 710, 1497
AspS9I GGNCC 9 cut(s) 81, 218, 439, 750, 964, 1026, 1223, 1865, 2266
AsuC2I CCSGG 1 cut(s) 1560
AsuHPI GGTGA 5 cut(s) 1264, 1321, 1669, 2249, 2251
AsuII TTCGAA 1 cut(s) 1297
AsuNHI GCTAGC 1 cut(s) 1279
AvaII GGWCC 7 cut(s) 81, 218, 439, 1026, 1223, 1865, 2266
AxyI CCTNAGG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 1879
BalI TGGCCA 1 cut(s) 1265
BanI GGYRCC 3 cut(s) 1662, 1876, 2312
BanII GRGCYC 2 cut(s) 184, 2460
BauI CACGAG 3 cut(s) 123, 1017, 1430
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 184, 2460
BbvCI CCTCAGC 1 cut(s) 1681
BbvI GCAGC 6 cut(s) 823, 905, 925, 1295, 1851, 2046
BccI CCATC 7 cut(s) 316, 491, 1243, 1274, 1682, 1856, 2035
BceAI ACGGC 1 cut(s) 205
BciT130I CCWGG 5 cut(s) 259, 865, 1596, 1689, 2133
BciVI GTATCC 2 cut(s) 420, 1511
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 1 cut(s) 1560
BcoDI GTCTC 6 cut(s) 294, 669, 763, 797, 1282, 1722
BcuI ACTAGT 2 cut(s) 1796, 2018
BfmI CTRYAG 3 cut(s) 691, 939, 1080
BfuI GTATCC 2 cut(s) 420, 1511
BglII AGATCT 1 cut(s) 1963
BisI GCNGC 9 cut(s) 43, 377, 380, 812, 894, 939, 1284, 1840, 2035
BlpI GCTNAGC 1 cut(s) 1806
BlsI GCNGC 9 cut(s) 44, 378, 381, 813, 895, 940, 1285, 1841, 2036
BmcAI AGTACT 1 cut(s) 2501
Bme1390I CCNGG 6 cut(s) 259, 865, 1560, 1596, 1689, 2133
Bme18I GGWCC 7 cut(s) 81, 218, 439, 1026, 1223, 1865, 2266
BmgT120I GGNCC 9 cut(s) 81, 218, 439, 750, 964, 1026, 1223, 1865, 2266
BmiI GGNNCC 7 cut(s) 14, 117, 1112, 1316, 1664, 1878, 2314
BmrFI CCNGG 6 cut(s) 259, 865, 1560, 1596, 1689, 2133
BmrI ACTGGG 1 cut(s) 1350
BmsI GCATC 8 cut(s) 302, 442, 1708, 1962, 2230, 2389, 2406, 2433
BmtI GCTAGC 1 cut(s) 1283
BmuI ACTGGG 1 cut(s) 1350
BoxI GACNNNNGTC 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 1681
Bpu1102I GCTNAGC 1 cut(s) 1806
Bpu14I TTCGAA 1 cut(s) 1297
BpuEI CTTGAG 1 cut(s) 2474
BpuMI CCSGG 1 cut(s) 1560
BsaBI GATNNNNATC 4 cut(s) 431, 1460, 1698, 1821
BsaJI CCNNGG 6 cut(s) 231, 280, 1186, 1405, 1688, 2262
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bse118I RCCGGY 1 cut(s) 1879
Bse1I ACTGG 6 cut(s) 923, 1225, 1243, 1345, 1736, 2004
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 2 cut(s) 496, 852
Bse8I GATNNNNATC 4 cut(s) 431, 1460, 1698, 1821
BseBI CCWGG 5 cut(s) 259, 865, 1596, 1689, 2133
BseDI CCNNGG 6 cut(s) 231, 280, 1186, 1405, 1688, 2262
BseJI GATNNNNATC 4 cut(s) 431, 1460, 1698, 1821
BseMI GCAATG 2 cut(s) 496, 852
BseMII CTCAG 8 cut(s) 185, 530, 728, 1695, 1717, 1752, 1820, 2473
BseNI ACTGG 6 cut(s) 923, 1225, 1243, 1345, 1736, 2004
BsePI GCGCGC 2 cut(s) 171, 455
BseRI GAGGAG 5 cut(s) 148, 248, 312, 350, 385
BseSI GKGCMC 1 cut(s) 1879
BseXI GCAGC 6 cut(s) 823, 905, 925, 1295, 1851, 2046
BseYI CCCAGC 1 cut(s) 1106
Bsh1236I CGCG 4 cut(s) 113, 173, 457, 710
BshFI GGCC 5 cut(s) 192, 257, 751, 965, 1265
BshNI GGYRCC 3 cut(s) 1662, 1876, 2312
BsiHKAI GWGCWC 2 cut(s) 184, 2460
BsiSI CCGG 4 cut(s) 102, 216, 1560, 1880
BsmAI GTCTC 6 cut(s) 294, 669, 763, 797, 1282, 1722
BsmBI CGTCTC 1 cut(s) 669
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 5 cut(s) 192, 257, 751, 965, 1265
Bsp119I TTCGAA 1 cut(s) 1297
Bsp1286I GDGCHC 3 cut(s) 184, 1879, 2460
Bsp1407I TGTACA 1 cut(s) 1458
Bsp1720I GCTNAGC 1 cut(s) 1806
Bsp19I CCATGG 3 cut(s) 1186, 1405, 2262
BspANI GGCC 5 cut(s) 192, 257, 751, 965, 1265
BspCNI CTCAG 8 cut(s) 186, 531, 729, 1694, 1716, 1753, 1819, 2472
BspFNI CGCG 4 cut(s) 113, 173, 457, 710
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 7 cut(s) 14, 117, 1112, 1316, 1664, 1878, 2314
BspMAI CTGCAG 1 cut(s) 943
BspOI GCTAGC 1 cut(s) 1283
BspPI GGATC 2 cut(s) 1830, 2190
BspT104I TTCGAA 1 cut(s) 1297
BspT107I GGYRCC 3 cut(s) 1662, 1876, 2312
BsrDI GCAATG 2 cut(s) 496, 852
BsrFI RCCGGY 1 cut(s) 1879
BsrGI TGTACA 1 cut(s) 1458
BsrI ACTGG 6 cut(s) 923, 1225, 1243, 1345, 1736, 2004
BssAI RCCGGY 1 cut(s) 1879
BssECI CCNNGG 6 cut(s) 231, 280, 1186, 1405, 1688, 2262
BssHII GCGCGC 2 cut(s) 171, 455
BssNAI GTATAC 1 cut(s) 682
BssSI CACGAG 3 cut(s) 123, 1017, 1430
BssT1I CCWWGG 3 cut(s) 1186, 1405, 2262
Bst1107I GTATAC 1 cut(s) 682
Bst2BI CACGAG 3 cut(s) 123, 1017, 1430
Bst2UI CCWGG 5 cut(s) 259, 865, 1596, 1689, 2133
Bst4CI ACNGT 3 cut(s) 1723, 2395, 2411
Bst6I CTCTTC 1 cut(s) 2226
BstAUI TGTACA 1 cut(s) 1458
BstBI TTCGAA 1 cut(s) 1297
BstC8I GCNNGC 6 cut(s) 173, 291, 457, 920, 1281, 1752
BstDEI CTNAG 8 cut(s) 194, 539, 737, 1681, 1703, 1761, 1806, 2459
BstDSI CCRYGG 4 cut(s) 280, 1186, 1405, 2262
BstEII GGTNACC 1 cut(s) 2119
BstFNI CGCG 4 cut(s) 113, 173, 457, 710
BstHHI GCGC 6 cut(s) 173, 175, 457, 459, 710, 1497
BstMAI GTCTC 6 cut(s) 294, 669, 763, 797, 1282, 1722
BstMWI GCNNNNNNNGC 7 cut(s) 42, 179, 244, 535, 565, 935, 2443
BstNI CCWGG 5 cut(s) 259, 865, 1596, 1689, 2133
BstNSI RCATGY 2 cut(s) 1125, 1512
BstPAI GACNNNNGTC 1 cut(s) 306
BstPI GGTNACC 1 cut(s) 2119
BstSCI CCNGG 6 cut(s) 257, 863, 1558, 1594, 1687, 2131
BstSFI CTRYAG 3 cut(s) 691, 939, 1080
BstSLI GKGCMC 1 cut(s) 1879
BstUI CGCG 4 cut(s) 113, 173, 457, 710
BstV1I GCAGC 6 cut(s) 823, 905, 925, 1295, 1851, 2046
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 1963
BstXI CCANNNNNNTGG 1 cut(s) 1659
BstYI RGATCY 1 cut(s) 1963
BstZ17I GTATAC 1 cut(s) 682
Bsu36I CCTNAGG 1 cut(s) 194
BsuI GTATCC 2 cut(s) 420, 1511
BsuRI GGCC 5 cut(s) 192, 257, 751, 965, 1265
BtgI CCRYGG 4 cut(s) 280, 1186, 1405, 2262
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 5 cut(s) 659, 794, 995, 1218, 1711
Cac8I GCNNGC 6 cut(s) 173, 291, 457, 920, 1281, 1752
CaiI CAGNNNCTG 2 cut(s) 736, 1688
CciI TCATGA 1 cut(s) 415
CfoI GCGC 6 cut(s) 173, 175, 457, 459, 710, 1497
Cfr10I RCCGGY 1 cut(s) 1879
Cfr13I GGNCC 9 cut(s) 81, 218, 439, 750, 964, 1026, 1223, 1865, 2266
CseI GACGC 1 cut(s) 1744
Csp6I GTAC 4 cut(s) 1459, 1606, 2022, 2500
CviQI GTAC 4 cut(s) 1459, 1606, 2022, 2500
DdeI CTNAG 8 cut(s) 194, 539, 737, 1681, 1703, 1761, 1806, 2459
DraI TTTAAA 1 cut(s) 2152
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 1263
Eam1104I CTCTTC 1 cut(s) 2226
EarI CTCTTC 1 cut(s) 2226
EciI GGCGGA 2 cut(s) 108, 341
Ecl136II GAGCTC 2 cut(s) 182, 2458
Eco130I CCWWGG 3 cut(s) 1186, 1405, 2262
Eco24I GRGCYC 2 cut(s) 184, 2460
Eco47I GGWCC 7 cut(s) 81, 218, 439, 1026, 1223, 1865, 2266
Eco53kI GAGCTC 2 cut(s) 182, 2458
Eco57I CTGAAG 5 cut(s) 161, 603, 730, 770, 2459
Eco81I CCTNAGG 1 cut(s) 194
Eco91I GGTNACC 1 cut(s) 2119
EcoICRI GAGCTC 2 cut(s) 182, 2458
EcoO109I RGGNCCY 1 cut(s) 1026
EcoO65I GGTNACC 1 cut(s) 2119
EcoRI GAATTC 2 cut(s) 1242, 2486
EcoRII CCWGG 5 cut(s) 257, 863, 1594, 1687, 2131
EcoT14I CCWWGG 3 cut(s) 1186, 1405, 2262
EcoT22I ATGCAT 1 cut(s) 317
EcoT38I GRGCYC 2 cut(s) 184, 2460
ErhI CCWWGG 3 cut(s) 1186, 1405, 2262
Esp3I CGTCTC 1 cut(s) 669
FalI AAGNNNNNCTT 5 cut(s) 648, 680, 1953, 1985, 2486
FauNDI CATATG 1 cut(s) 1870
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 3 cut(s) 681, 1386, 2226
Fnu4HI GCNGC 9 cut(s) 43, 377, 380, 812, 894, 939, 1284, 1840, 2035
FriOI GRGCYC 2 cut(s) 184, 2460
Fsp4HI GCNGC 9 cut(s) 43, 377, 380, 812, 894, 939, 1284, 1840, 2035
GlaI GCGC 6 cut(s) 172, 174, 456, 458, 709, 1496
GluI GCNGC 9 cut(s) 43, 377, 380, 812, 894, 939, 1284, 1840, 2035
GsaI CCCAGC 1 cut(s) 1110
HaeIII GGCC 5 cut(s) 192, 257, 751, 965, 1265
HapII CCGG 4 cut(s) 102, 216, 1560, 1880
HgaI GACGC 1 cut(s) 1744
HhaI GCGC 6 cut(s) 173, 175, 457, 459, 710, 1497
Hin6I GCGC 6 cut(s) 171, 173, 455, 457, 708, 1495
HinP1I GCGC 6 cut(s) 171, 173, 455, 457, 708, 1495
HincII GTYRAC 2 cut(s) 2250, 2299
HindII GTYRAC 2 cut(s) 2250, 2299
HindIII AAGCTT 2 cut(s) 149, 953
HinfI GANTC 9 cut(s) 89, 266, 329, 504, 727, 757, 2003, 2157, 2326
HpaII CCGG 4 cut(s) 102, 216, 1560, 1880
HphI GGTGA 5 cut(s) 1264, 1321, 1669, 2249, 2251
Hpy188III TCNNGA 2 cut(s) 400, 416
Hpy99I CGWCG 3 cut(s) 73, 76, 79
HpyAV CCTTC 8 cut(s) 3, 136, 397, 470, 754, 1039, 1223, 1293
HpyCH4III ACNGT 3 cut(s) 1723, 2395, 2411
HpyCH4IV ACGT 3 cut(s) 26, 678, 1389
HpyCH4V TGCA 9 cut(s) 315, 523, 551, 845, 941, 1073, 1839, 2243, 2336
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 179, 244, 535, 565, 935, 2443
HpyF3I CTNAG 8 cut(s) 194, 539, 737, 1681, 1703, 1761, 1806, 2459
HpySE526I ACGT 3 cut(s) 26, 678, 1389
HspAI GCGC 6 cut(s) 171, 173, 455, 457, 708, 1495
Ksp22I TGATCA 1 cut(s) 60
LmnI GCTCC 7 cut(s) 12, 33, 235, 935, 1320, 1934, 2434
Lsp1109I GCAGC 6 cut(s) 823, 905, 925, 1295, 1851, 2046
LweI GCATC 8 cut(s) 302, 442, 1708, 1962, 2230, 2389, 2406, 2433
MaeII ACGT 3 cut(s) 26, 678, 1389
MaeIII GTNAC 5 cut(s) 27, 989, 1124, 2119, 2462
MfeI CAATTG 3 cut(s) 650, 805, 1086
MflI RGATCY 1 cut(s) 1963
MhlI GDGCHC 3 cut(s) 184, 1879, 2460
MlsI TGGCCA 1 cut(s) 1265
MluNI TGGCCA 1 cut(s) 1265
MlyI GAGTC 6 cut(s) 98, 275, 323, 736, 2166, 2320
MmeI TCCRAC 4 cut(s) 120, 704, 800, 1705
Mox20I TGGCCA 1 cut(s) 1265
Mph1103I ATGCAT 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 1265
MslI CAYNNNNRTG 7 cut(s) 52, 320, 787, 1000, 1657, 1704, 2056
Msp20I TGGCCA 1 cut(s) 1265
MspI CCGG 4 cut(s) 102, 216, 1560, 1880
MspR9I CCNGG 6 cut(s) 259, 865, 1560, 1596, 1689, 2133
MunI CAATTG 3 cut(s) 650, 805, 1086
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 5 cut(s) 259, 865, 1596, 1689, 2133
MvnI CGCG 4 cut(s) 113, 173, 457, 710
MwoI GCNNNNNNNGC 7 cut(s) 42, 179, 244, 535, 565, 935, 2443
NciI CCSGG 1 cut(s) 1560
NcoI CCATGG 3 cut(s) 1186, 1405, 2262
NdeI CATATG 1 cut(s) 1870
NheI GCTAGC 1 cut(s) 1279
NlaIV GGNNCC 7 cut(s) 14, 117, 1112, 1316, 1664, 1878, 2314
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 3 cut(s) 27, 1124, 2462
NsiI ATGCAT 1 cut(s) 317
NspI RCATGY 2 cut(s) 1125, 1512
NspV TTCGAA 1 cut(s) 1297
OliI CACNNNNGTG 1 cut(s) 787
PagI TCATGA 1 cut(s) 415
PauI GCGCGC 2 cut(s) 171, 455
PciI ACATGT 1 cut(s) 1508
PcsI WCGNNNNNNNCGW 4 cut(s) 74, 398, 1015, 1283
PctI GAATGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 504, 757, 2003
PkrI GCNGC 9 cut(s) 44, 378, 381, 813, 895, 940, 1285, 1841, 2036
PleI GAGTC 6 cut(s) 97, 274, 323, 735, 2165, 2320
PpsI GAGTC 6 cut(s) 97, 274, 323, 735, 2165, 2320
PpuMI RGGWCCY 1 cut(s) 1026
PscI ACATGT 1 cut(s) 1508
PshAI GACNNNNGTC 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 1053
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 2 cut(s) 184, 2460
Psp5II RGGWCCY 1 cut(s) 1026
Psp6I CCWGG 5 cut(s) 257, 863, 1594, 1687, 2131
PspEI GGTNACC 1 cut(s) 2119
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 5 cut(s) 257, 863, 1594, 1687, 2131
PspN4I GGNNCC 7 cut(s) 14, 117, 1112, 1316, 1664, 1878, 2314
PspPI GGNCC 9 cut(s) 81, 218, 439, 750, 964, 1026, 1223, 1865, 2266
PspPPI RGGWCCY 1 cut(s) 1026
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 2 cut(s) 736, 1688
PsuI RGATCY 1 cut(s) 1963
PteI GCGCGC 2 cut(s) 171, 455
RsaI GTAC 4 cut(s) 1460, 1607, 2023, 2501
RsaNI GTAC 4 cut(s) 1459, 1606, 2022, 2500
RseI CAYNNNNRTG 7 cut(s) 52, 320, 787, 1000, 1657, 1704, 2056
SacI GAGCTC 2 cut(s) 184, 2460
SatI GCNGC 9 cut(s) 43, 377, 380, 812, 894, 939, 1284, 1840, 2035
Sau96I GGNCC 9 cut(s) 81, 218, 439, 750, 964, 1026, 1223, 1865, 2266
ScaI AGTACT 1 cut(s) 2501
SchI GAGTC 6 cut(s) 98, 275, 323, 736, 2166, 2320
ScrFI CCNGG 6 cut(s) 259, 865, 1560, 1596, 1689, 2133
SduI GDGCHC 3 cut(s) 184, 1879, 2460
SfaNI GCATC 8 cut(s) 302, 442, 1708, 1962, 2230, 2389, 2406, 2433
SfcI CTRYAG 3 cut(s) 691, 939, 1080
SfuI TTCGAA 1 cut(s) 1297
SinI GGWCC 7 cut(s) 81, 218, 439, 1026, 1223, 1865, 2266
SmiMI CAYNNNNRTG 7 cut(s) 52, 320, 787, 1000, 1657, 1704, 2056
SmlI CTYRAG 1 cut(s) 2453
SmoI CTYRAG 1 cut(s) 2453
SpeI ACTAGT 2 cut(s) 1796, 2018
SstI GAGCTC 2 cut(s) 184, 2460
StyD4I CCNGG 6 cut(s) 257, 863, 1558, 1594, 1687, 2131
StyI CCWWGG 3 cut(s) 1186, 1405, 2262
TaaI ACNGT 3 cut(s) 1723, 2395, 2411
TaiI ACGT 3 cut(s) 29, 681, 1392
TatI WGTACW 4 cut(s) 1458, 1605, 2021, 2499
TauI GCSGC 3 cut(s) 45, 379, 382
TfiI GAWTC 3 cut(s) 504, 757, 2003
TscAI CASTG 5 cut(s) 666, 794, 1002, 1225, 1711
TseFI GTSAC 3 cut(s) 27, 1124, 2462
TseI GCWGC 6 cut(s) 811, 893, 938, 1283, 1839, 2034
Tsp45I GTSAC 3 cut(s) 27, 1124, 2462
TspDTI ATGAA 9 cut(s) 190, 404, 585, 1021, 1091, 1700, 1764, 1778, 2181
TspGWI ACGGA 4 cut(s) 46, 172, 265, 297
TspRI CASTG 5 cut(s) 666, 794, 1002, 1225, 1711
VpaK11BI GGWCC 7 cut(s) 81, 218, 439, 1026, 1223, 1865, 2266
VspI ATTAAT 1 cut(s) 1053
XapI RAATTY 6 cut(s) 465, 869, 1160, 1208, 1242, 2486
XceI RCATGY 2 cut(s) 1125, 1512
XcmI CCANNNNNNNNNTGG 1 cut(s) 2288
XmiI GTMKAC 3 cut(s) 681, 1386, 2226
XmnI GAANNNNTTC 1 cut(s) 411
ZrmI AGTACT 1 cut(s) 2501
Zsp2I ATGCAT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.