MD15G1222800.v1.1

Respiratory burst oxidase homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003315
Physical Location & Seq
Reverse (-)
18082033 .. 18086949
4917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1222800.v1.1.491

Sequence Viewer

Length: 2841 bp
ATGCCAACCGCGTCGTTTAGCTCGAAGCGGTCGAGTTACACCCACGGGTTTGAGTTACCGGGAAACTCCTCGCCGTCGTCGGTCGGTGGTGCAATGCTGCCGAAGTTTCTCAACGACCTCAACCAGGACTTGGTCGAAGTCACATTGGAGCTCGACGATGACGACGACGGCTCAATTGTCGTGTGCAGCGTCGCTCCCGCAAATGATGATTTGACGTCGTCGGAGGCCGCGGCGGCGAACGGGATTTTACGGAGGAGTCTTTCGGCGACGTCGAGGAGTATTCGGCGGACGTTTGGGTGGTTGAAATCGGCGTCTTCGAGGACGACGGCGTCGAACTCGGAGTCCGTGGCAGAGTTCACTCTGCCGGCTCGCGAGGCGAGGAGGATGAAGGCCAAGCTTCAACGGACGAGATCGAGCGCGCAGAGAGCTCTCAATGGGTTGAGATTCATCAGCAAGACGACTGCCGGAGGAAGTGACGCGGCGGAGCAGTGGCAGCAAGTGGAGGCGAGGTTCGAGTCGCTCGCCAAGGACGGCATGCTCGCCAGTGCAGACTTCGGTGAATGTATAGGGATGGGGGACTCGGAGGAGTTTGCGGTGGGGATATTTGATGCATTGGCGAGAAGGCGGCGGCAGAAGATAAGCAAAATTACGAAAGAGGAGCTTCGCGACTTTTGGTTGCAGATTTCCGACAACAGCTTCGACGCGCGCCTTCAAATCTTCTTTGACATGGCGGATAGCAATGAAGATGGAAGAATCACAAGGGAAGGAGTTCGAGAGCTTATAATGCTCAGTGCTTCTGCAAACAAGCTACCTAAGCTGAAAGAACAAGCTGAAGATTATGCTTCATTAATAATGGAAGAACTTGATCCCGAAAACTTCGGTTACATTGAGTTATGGCAATTGGAAGCATTGCTTCTACAAAGAGACGTGTACATAAACTACAGCAGACCATTGAGCACAGCAAGTGTATCTTGGAGTCAGAACCTCAGTTCCTTGAAGCCCAAGAATCTGGTCCGGAGACTGAGCCGTTCACTTCAGTGTCTCGTACTCGAGAACTGGCAGGGCAGTTGGATTCTACTGCTGTGGGTTGTTGCAATGGCTTCCCTTTTTGGCTGGAAATTCTACCAATACAGAAAAAATAGGGCTGCATTTCAGGTCATGCACTATTGCTTGCCAGTGGCCAAAGGGGCTGCAGAGACTCTGAAGCTTAACATGGCTCTCATCCTCCTACCGGTTTGTCGAAACATGCTGACATGGCTTCGATCCACAAGAGCTAGGTGCTTCATCCCTTTTGATGACAACATTAATTTCCACAAGATAATTGCATTTGCTATAGCAATTGGAATAATCATTCACGCCGGGAACCATCTGGCATGCGACTTTCCTCGCATAGTAAACTCATCGCCGGAAAAATTCGCACCGCTATCCTCAGATTTCCACAACAAAAAGCCCACCTACGAATTCCTACTCACCGGAACTGAAGGCACGACTGGAATTTTGATGGTGATTTTAATAGCCATCTCATTCACTCTAGCAGCGCGTAGATTTCGGAGGAACATGGTGAGGCTTCCTGCTCCATTCAACAAGTTGACTGGGTTTAATGCGTTCTGGTATTCTCATCATCTGCTAGGTCTTGTCTACATATTGCTAATCATACATGGAAGCTGCTTGTATTTAGCCCACAAGTGGTACGAGAAAACGACATGGATGTACATCTCTGTTCCATTGTTGCTCTATGTGGCCGAACGCAGTCTGCGAACATGTAGATCGAAGCATTATTCTGTTAAGATGTTAAAGGTTTCGGTCCTACCCGGAAATGTGATTAGCTTAATCATGTCCAAACCACCTGGATTTAAGTACAAAAGTGGGCAGTACATATTTCTACAATGCCCTACAATCTCCTCATTTGAATGGCATCCATTTTCCATTACCTCAGCGCCAGGAGATGAGTACCTCAGCGTTCACATCCGAACAGTAGGAGACTGGACTCGAGAATTGAAGCGAGCCATAGCGGAGGAAGATGATTCATCCCCTGCAGAACATTCAGCAAGATTTGTTCAGATGCGAAATCTGGATCGGAGAGGCCAACCCAGATTGTTTATCGACGGTCCATATGGGGCACCAGCGCAGGACTACCAGAACTACGATGTCTTGCTCCTCGTGGGGCTTGGGATTGGAGCTACCCCTTTCATAAGCATTCTTCGAGATCTCCTAAACAGCACAAGAACAGCAGAAGAACAAATGGTACAGTTCATAACATCCGACTCTTTTGAGTACAAACATTCAGATTCAAATACAGAAACTAGTAGATCAGACGACAGCCAGAATAGTTTTACGTCTTCAAGTTTGACGCCCGGTGACAAGAAGAAATCGCAGAGGACTATGAATGCACACTTCTATTGGGTTACCAGAGAGCCTGGCTCTTTTGAGTGGTTTAAAGGAGTCATGGATGAGGTTGCAGAAATGGATCACAAAGGTCAGATTGAACTACACAACTATCTTACGAGTGTCTACGAAGAGGGTGACGCTAGGTCGACTCTAATCACCATGGTCCAAGCTCTAAACCACGCCAAACATGGCGTCGACATCCTATCTGGCACCCGAGTAAGAACTCACTTCGCAAGACCGGATTGGAAAGAAGTGTTTACCAAGATAGCTTCCAAGCATCCGTATTCAACAGTAGGTAAAGATATTCTACCACAACCGAATTTTTATGTTAAAAACATAAACCCTAATCATGCATCTAGTAGAGAACCGAAACAAAGAACCGAGAGATTAAACGAATCAGCTAATTTGCAGGGGTATTCTACTGTGGGATGCCGATGTTGGCAAAGGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

947

Amino Acids

106.97

Weight (kDa)

8.91

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NADPH_Ox PF08414 132 - 235 3.7e-39 Respiratory burst NADPH oxidase
Ferric_reduct PF01794 404 - 551 1.1e-09 Ferric reductase like transmembrane component
FAD_binding_8 PF08022 593 - 709 7.7e-33 FAD-binding domain
NAD_binding_6 PF08030 715 - 895 4.1e-40 Ferric reductase NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AasI GACNNNNNNGTC 1 cut(s) 328
AatII GACGTC 2 cut(s) 218, 272
AccB1I GGYRCC 2 cut(s) 2119, 2597
AccB7I CCANNNNNTGG 1 cut(s) 130
AccI GTMKAC 4 cut(s) 1638, 2511, 2534, 2583
AccII CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
AccIII TCCGGA 1 cut(s) 1014
AclWI GGATC 4 cut(s) 860, 1257, 2082, 2475
AcoI YGGCCR 2 cut(s) 1179, 1740
AcsI RAATTY 5 cut(s) 1118, 1412, 1460, 1494, 2707
AcuI CTGAAG 4 cut(s) 852, 1019, 1223, 1500
AcyI GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
AfaI GTAC 9 cut(s) 932, 1047, 1691, 1712, 1859, 1874, 1952, 2247, 2276
AfiI CCNNNNNNNGG 4 cut(s) 124, 130, 1231, 1686
AflIII ACRYGT 2 cut(s) 927, 1760
AgeI ACCGGT 1 cut(s) 1231
AhdI GACNNNNNGTC 1 cut(s) 2530
AhlI ACTAGT 1 cut(s) 2303
AjiI CACGTC 1 cut(s) 928
AjnI CCWGG 4 cut(s) 123, 1846, 1939, 2416
AleI CACNNNNGTG 1 cut(s) 1036
AloI GAACNNNNNNTCC 6 cut(s) 326, 358, 494, 526, 974, 1006
Alw21I GWGCWC 3 cut(s) 153, 430, 959
Alw26I GTCTC 5 cut(s) 918, 1012, 1046, 1190, 1974
AlwI GGATC 4 cut(s) 860, 1257, 2082, 2475
Ama87I CYCGRG 3 cut(s) 1049, 1989, 2601
Aor13HI TCCGGA 1 cut(s) 1014
AoxI GGCC 5 cut(s) 225, 390, 1179, 1740, 2083
ApeKI GCWGC 7 cut(s) 97, 186, 493, 1145, 1190, 1535, 1665
ApoI RAATTY 5 cut(s) 1118, 1412, 1460, 1494, 2707
ArsI GACNNNNNNTTYG 8 cut(s) 326, 358, 680, 712, 1480, 1512, 2565, 2597
AseI ATTAAT 2 cut(s) 848, 1305
AsiGI ACCGGT 1 cut(s) 1231
Asp700I GAANNNNTTC 1 cut(s) 768
AspLEI GCGC 7 cut(s) 419, 421, 706, 708, 1540, 1939, 2128
AspS9I GGNCC 4 cut(s) 1012, 1804, 2108, 2551
AsuC2I CCSGG 4 cut(s) 60, 1360, 1812, 2355
AsuHPI GGTGA 7 cut(s) 569, 1462, 1516, 1573, 2369, 2534, 2536
AvaI CYCGRG 3 cut(s) 1049, 1989, 2601
AvaII GGWCC 4 cut(s) 1012, 1804, 2108, 2551
BaeGI GKGCMC 1 cut(s) 2122
BalI TGGCCA 1 cut(s) 1181
BanI GGYRCC 2 cut(s) 2119, 2597
BanII GRGCYC 2 cut(s) 153, 430
BauI CACGAG 1 cut(s) 2159
BbsI GAAGAC 2 cut(s) 306, 2331
Bbv12I GWGCWC 3 cut(s) 153, 430, 959
BbvCI CCTCAGC 2 cut(s) 1933, 1955
BbvI GCAGC 7 cut(s) 84, 198, 505, 1132, 1177, 1547, 1652
BccI CCATC 5 cut(s) 565, 740, 1374, 1495, 1526
BceAI ACGGC 5 cut(s) 58, 184, 342, 547, 1011
BciT130I CCWGG 4 cut(s) 125, 1848, 1941, 2418
BcnI CCSGG 4 cut(s) 60, 1360, 1812, 2355
BcoDI GTCTC 5 cut(s) 918, 1012, 1046, 1190, 1974
BcuI ACTAGT 1 cut(s) 2303
BfaI CTAG 6 cut(s) 1275, 1532, 1628, 2304, 2529, 2745
BfmI CTRYAG 4 cut(s) 940, 1191, 1332, 2034
BfoI RGCGCY 1 cut(s) 1940
BglI GCCNNNNNGGC 2 cut(s) 233, 1187
BglII AGATCT 1 cut(s) 2206
Bme1390I CCNGG 8 cut(s) 60, 125, 1360, 1812, 1848, 1941, 2355, 2418
Bme18I GGWCC 4 cut(s) 1012, 1804, 2108, 2551
BmeRI GACNNNNNGTC 1 cut(s) 2530
BmeT110I CYCGRG 3 cut(s) 1049, 1989, 2601
BmgBI CACGTC 1 cut(s) 928
BmgT120I GGNCC 4 cut(s) 1012, 1804, 2108, 2551
BmiI GGNNCC 3 cut(s) 1364, 2121, 2599
BmrFI CCNGG 8 cut(s) 60, 125, 1360, 1812, 1848, 1941, 2355, 2418
BmrI ACTGGG 1 cut(s) 1602
BmsI GCATC 6 cut(s) 598, 1924, 2052, 2674, 2750, 2807
BmuI ACTGGG 1 cut(s) 1602
BpiI GAAGAC 2 cut(s) 306, 2331
BplI GAGNNNNNCTC 2 cut(s) 2405, 2437
Bpu10I CCTNAGC 3 cut(s) 813, 1933, 1955
BpuMI CCSGG 4 cut(s) 60, 1360, 1812, 2355
BsaBI GATNNNNATC 1 cut(s) 1712
BsaHI GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
BsaJI CCNNGG 5 cut(s) 43, 228, 345, 525, 2547
BsaWI WCCGGW 4 cut(s) 1014, 1231, 1472, 2626
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 130, 1231, 1686
Bse118I RCCGGY 2 cut(s) 364, 1231
Bse1I ACTGG 6 cut(s) 543, 1061, 1175, 1495, 1597, 1988
Bse3DI GCAATG 4 cut(s) 99, 745, 908, 1101
Bse8I GATNNNNATC 1 cut(s) 1712
BseAI TCCGGA 1 cut(s) 1014
BseBI CCWGG 4 cut(s) 125, 1848, 1941, 2418
BseDI CCNNGG 5 cut(s) 43, 228, 345, 525, 2547
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 4 cut(s) 124, 130, 1231, 1686
BseMI GCAATG 4 cut(s) 99, 745, 908, 1101
BseMII CTCAG 6 cut(s) 802, 1000, 1013, 1443, 1947, 1969
BseNI ACTGG 6 cut(s) 543, 1061, 1175, 1495, 1597, 1988
BsePI GCGCGC 2 cut(s) 417, 704
BseRI GAGGAG 8 cut(s) 58, 268, 289, 394, 599, 671, 1891, 2147
BseSI GKGCMC 1 cut(s) 2122
BseXI GCAGC 7 cut(s) 84, 198, 505, 1132, 1177, 1547, 1652
BsgI GTGCAG 2 cut(s) 205, 567
Bsh1236I CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
Bsh1285I CGRYCG 2 cut(s) 32, 84
BshFI GGCC 5 cut(s) 227, 392, 1181, 1742, 2085
BshNI GGYRCC 2 cut(s) 2119, 2597
BshTI ACCGGT 1 cut(s) 1231
BsiEI CGRYCG 2 cut(s) 32, 84
BsiHKAI GWGCWC 3 cut(s) 153, 430, 959
BsiHKCI CYCGRG 3 cut(s) 1049, 1989, 2601
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 4 cut(s) 124, 130, 1231, 1686
BsmAI GTCTC 5 cut(s) 918, 1012, 1046, 1190, 1974
BsmBI CGTCTC 1 cut(s) 918
BsmFI GGGAC 1 cut(s) 590
BsmI GAATGC 2 cut(s) 2196, 2392
BsnI GGCC 5 cut(s) 227, 392, 1181, 1742, 2085
BsoBI CYCGRG 3 cut(s) 1049, 1989, 2601
Bsp1286I GDGCHC 4 cut(s) 153, 430, 959, 2122
Bsp13I TCCGGA 1 cut(s) 1014
Bsp1407I TGTACA 2 cut(s) 930, 1710
Bsp143I GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
Bsp19I CCATGG 1 cut(s) 2547
Bsp68I TCGCGA 2 cut(s) 372, 666
BspANI GGCC 5 cut(s) 227, 392, 1181, 1742, 2085
BspCNI CTCAG 6 cut(s) 801, 999, 1014, 1442, 1946, 1968
BspEI TCCGGA 1 cut(s) 1014
BspFNI CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
BspLI GGNNCC 3 cut(s) 1364, 2121, 2599
BspMAI CTGCAG 2 cut(s) 1195, 2038
BspPI GGATC 4 cut(s) 860, 1257, 2082, 2475
BspT107I GGYRCC 2 cut(s) 2119, 2597
BsrDI GCAATG 4 cut(s) 99, 745, 908, 1101
BsrFI RCCGGY 2 cut(s) 364, 1231
BsrGI TGTACA 2 cut(s) 930, 1710
BsrI ACTGG 6 cut(s) 543, 1061, 1175, 1495, 1597, 1988
BssAI RCCGGY 2 cut(s) 364, 1231
BssECI CCNNGG 5 cut(s) 43, 228, 345, 525, 2547
BssHII GCGCGC 2 cut(s) 417, 704
BssMI GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
BssNI GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
BssSI CACGAG 1 cut(s) 2159
BssT1I CCWWGG 2 cut(s) 525, 2547
Bst2BI CACGAG 1 cut(s) 2159
Bst2UI CCWGG 4 cut(s) 125, 1848, 1941, 2418
Bst4CI ACNGT 5 cut(s) 1975, 2108, 2250, 2680, 2812
Bst6I CTCTTC 1 cut(s) 2511
BstACI GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
BstAUI TGTACA 2 cut(s) 930, 1710
BstDEI CTNAG 7 cut(s) 788, 813, 986, 1022, 1429, 1933, 1955
BstDSI CCRYGG 4 cut(s) 43, 228, 345, 2547
BstEII GGTNACC 1 cut(s) 2404
BstENI CCTNNNNNAGG 1 cut(s) 122
BstFNI CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
BstH2I RGCGCY 1 cut(s) 1940
BstHHI GCGC 7 cut(s) 419, 421, 706, 708, 1540, 1939, 2128
BstKTI GATC 8 cut(s) 413, 868, 1265, 1769, 2077, 2209, 2312, 2470
BstMAI GTCTC 5 cut(s) 918, 1012, 1046, 1190, 1974
BstMBI GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
BstMCI CGRYCG 2 cut(s) 32, 84
BstMWI GCNNNNNNNGC 8 cut(s) 233, 374, 425, 493, 784, 814, 1187, 1255
BstNI CCWGG 4 cut(s) 125, 1848, 1941, 2418
BstNSI RCATGY 4 cut(s) 538, 1249, 1377, 1764
BstPI GGTNACC 1 cut(s) 2404
BstSCI CCNGG 8 cut(s) 58, 123, 1358, 1810, 1846, 1939, 2353, 2416
BstSFI CTRYAG 4 cut(s) 940, 1191, 1332, 2034
BstSLI GKGCMC 1 cut(s) 2122
BstUI CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
BstV1I GCAGC 7 cut(s) 84, 198, 505, 1132, 1177, 1547, 1652
BstV2I GAAGAC 2 cut(s) 306, 2331
BstX2I RGATCY 1 cut(s) 2206
BstXI CCANNNNNNTGG 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 2206
BsuRI GGCC 5 cut(s) 227, 392, 1181, 1742, 2085
BtgI CCRYGG 4 cut(s) 43, 228, 345, 2547
BtgZI GCGATG 1 cut(s) 1386
BtrI CACGTC 1 cut(s) 928
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 5 cut(s) 494, 550, 796, 1043, 1182
BtuMI TCGCGA 2 cut(s) 372, 666
CfoI GCGC 7 cut(s) 419, 421, 706, 708, 1540, 1939, 2128
Cfr10I RCCGGY 2 cut(s) 364, 1231
Cfr13I GGNCC 4 cut(s) 1012, 1804, 2108, 2551
Cfr42I CCGCGG 1 cut(s) 231
CseI GACGC 8 cut(s) 178, 300, 318, 485, 710, 2359, 2534, 2569
Csp6I GTAC 9 cut(s) 931, 1046, 1690, 1711, 1858, 1873, 1951, 2246, 2275
CspAI ACCGGT 1 cut(s) 1231
CviQI GTAC 9 cut(s) 931, 1046, 1690, 1711, 1858, 1873, 1951, 2246, 2275
DdeI CTNAG 7 cut(s) 788, 813, 986, 1022, 1429, 1933, 1955
DpnI GATC 8 cut(s) 412, 867, 1264, 1768, 2076, 2208, 2311, 2469
DpnII GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
DraI TTTAAA 1 cut(s) 2437
DrdI GACNNNNNNGTC 1 cut(s) 328
DriI GACNNNNNGTC 1 cut(s) 2530
DseDI GACNNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 2 cut(s) 1179, 1740
Eam1104I CTCTTC 1 cut(s) 2511
Eam1105I GACNNNNNGTC 1 cut(s) 2530
EarI CTCTTC 1 cut(s) 2511
EciI GGCGGA 3 cut(s) 301, 497, 746
Ecl136II GAGCTC 2 cut(s) 151, 428
Eco130I CCWWGG 2 cut(s) 525, 2547
Eco24I GRGCYC 2 cut(s) 153, 430
Eco47I GGWCC 4 cut(s) 1012, 1804, 2108, 2551
Eco53kI GAGCTC 2 cut(s) 151, 428
Eco57I CTGAAG 4 cut(s) 852, 1019, 1223, 1500
Eco88I CYCGRG 3 cut(s) 1049, 1989, 2601
Eco91I GGTNACC 1 cut(s) 2404
EcoICRI GAGCTC 2 cut(s) 151, 428
EcoNI CCTNNNNNAGG 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 2404
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 4 cut(s) 123, 1846, 1939, 2416
EcoT14I CCWWGG 2 cut(s) 525, 2547
EcoT22I ATGCAT 2 cut(s) 613, 2743
EcoT38I GRGCYC 2 cut(s) 153, 430
ErhI CCWWGG 2 cut(s) 525, 2547
Esp3I CGTCTC 1 cut(s) 918
FalI AAGNNNNNCTT 6 cut(s) 645, 677, 897, 929, 955, 987
FaqI GGGAC 1 cut(s) 590
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 1 cut(s) 2113
FblI GTMKAC 4 cut(s) 1638, 2511, 2534, 2583
FriOI GRGCYC 2 cut(s) 153, 430
FspBI CTAG 6 cut(s) 1275, 1532, 1628, 2304, 2529, 2745
GlaI GCGC 7 cut(s) 418, 420, 705, 707, 1539, 1938, 2127
HaeII RGCGCY 1 cut(s) 1940
HaeIII GGCC 5 cut(s) 227, 392, 1181, 1742, 2085
HgaI GACGC 8 cut(s) 178, 300, 318, 485, 710, 2359, 2534, 2569
HhaI GCGC 7 cut(s) 419, 421, 706, 708, 1540, 1939, 2128
Hin1I GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
Hin6I GCGC 7 cut(s) 417, 419, 704, 706, 1538, 1937, 2126
HinP1I GCGC 7 cut(s) 417, 419, 704, 706, 1538, 1937, 2126
HincII GTYRAC 3 cut(s) 1590, 2535, 2584
HindII GTYRAC 3 cut(s) 1590, 2535, 2584
HindIII AAGCTT 2 cut(s) 395, 1205
HphI GGTGA 7 cut(s) 569, 1462, 1516, 1573, 2369, 2534, 2536
Hpy188III TCNNGA 9 cut(s) 371, 665, 773, 869, 1015, 1051, 1991, 2072, 2204
HpyAV CCTTC 5 cut(s) 382, 615, 719, 758, 1475
HpyCH4III ACNGT 5 cut(s) 1975, 2108, 2250, 2680, 2812
HpyCH4IV ACGT 5 cut(s) 215, 269, 290, 927, 2336
HpyF10VI GCNNNNNNNGC 8 cut(s) 233, 374, 425, 493, 784, 814, 1187, 1255
HpyF3I CTNAG 7 cut(s) 788, 813, 986, 1022, 1429, 1933, 1955
HpySE526I ACGT 5 cut(s) 215, 269, 290, 927, 2336
Hsp92I GRCGYC 6 cut(s) 215, 269, 311, 329, 2351, 2580
HspAI GCGC 7 cut(s) 417, 419, 704, 706, 1538, 1937, 2126
Kpn2I TCCGGA 1 cut(s) 1014
KroI GCCGGC 1 cut(s) 364
KroNI GCCGGC 1 cut(s) 366
KspI CCGCGG 1 cut(s) 231
Kzo9I GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
LmnI GCTCC 8 cut(s) 148, 199, 484, 658, 1579, 2160, 2177, 2835
Lsp1109I GCAGC 7 cut(s) 84, 198, 505, 1132, 1177, 1547, 1652
LweI GCATC 6 cut(s) 598, 1924, 2052, 2674, 2750, 2807
MaeI CTAG 6 cut(s) 1275, 1532, 1628, 2304, 2529, 2745
MaeII ACGT 5 cut(s) 215, 269, 290, 927, 2336
MaeIII GTNAC 8 cut(s) 35, 54, 139, 473, 881, 2357, 2404, 2522
MalI GATC 8 cut(s) 412, 867, 1264, 1768, 2076, 2208, 2311, 2469
MboI GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
MfeI CAATTG 3 cut(s) 174, 899, 1338
MflI RGATCY 1 cut(s) 2206
MhlI GDGCHC 4 cut(s) 153, 430, 959, 2122
MlsI TGGCCA 1 cut(s) 1181
MluNI TGGCCA 1 cut(s) 1181
MmeI TCCRAC 4 cut(s) 201, 711, 1049, 2286
Mox20I TGGCCA 1 cut(s) 1181
Mph1103I ATGCAT 2 cut(s) 613, 2743
MroI TCCGGA 1 cut(s) 1014
MroNI GCCGGC 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 768
MscI TGGCCA 1 cut(s) 1181
MslI CAYNNNNRTG 2 cut(s) 1036, 1909
Msp20I TGGCCA 1 cut(s) 1181
MspA1I CMGCKG 1 cut(s) 230
MspR9I CCNGG 8 cut(s) 60, 125, 1360, 1812, 1848, 1941, 2355, 2418
MunI CAATTG 3 cut(s) 174, 899, 1338
Mva1269I GAATGC 2 cut(s) 2196, 2392
MvaI CCWGG 4 cut(s) 125, 1848, 1941, 2418
MvnI CGCG 9 cut(s) 11, 230, 372, 419, 479, 666, 704, 706, 1540
MwoI GCNNNNNNNGC 8 cut(s) 233, 374, 425, 493, 784, 814, 1187, 1255
NaeI GCCGGC 1 cut(s) 366
NciI CCSGG 4 cut(s) 60, 1360, 1812, 2355
NcoI CCATGG 1 cut(s) 2547
NdeI CATATG 1 cut(s) 2113
NdeII GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
NgoMIV GCCGGC 1 cut(s) 364
NlaIV GGNNCC 3 cut(s) 1364, 2121, 2599
NmuCI GTSAC 4 cut(s) 139, 473, 2357, 2522
NruI TCGCGA 2 cut(s) 372, 666
NsiI ATGCAT 2 cut(s) 613, 2743
NspI RCATGY 4 cut(s) 538, 1249, 1377, 1764
OliI CACNNNNGTG 1 cut(s) 1036
PaeI GCATGC 2 cut(s) 538, 1377
PaeR7I CTCGAG 2 cut(s) 1049, 1989
PauI GCGCGC 2 cut(s) 417, 704
PciI ACATGT 1 cut(s) 1760
PcsI WCGNNNNNNNCGW 9 cut(s) 20, 29, 159, 162, 186, 269, 314, 329, 1753
PctI GAATGC 2 cut(s) 2196, 2392
PdiI GCCGGC 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 7 cut(s) 444, 753, 1006, 1072, 2024, 2288, 2783
PflFI GACNNNGTC 3 cut(s) 131, 217, 328
PflMI CCANNNNNTGG 1 cut(s) 130
PinAI ACCGGT 1 cut(s) 1231
PscI ACATGT 1 cut(s) 1760
PshBI ATTAAT 2 cut(s) 848, 1305
PsiI TTATAA 1 cut(s) 782
Psp124BI GAGCTC 2 cut(s) 153, 430
Psp6I CCWGG 4 cut(s) 123, 1846, 1939, 2416
PspEI GGTNACC 1 cut(s) 2404
PspGI CCWGG 4 cut(s) 123, 1846, 1939, 2416
PspN4I GGNNCC 3 cut(s) 1364, 2121, 2599
PspPI GGNCC 4 cut(s) 1012, 1804, 2108, 2551
PsrI GAACNNNNNNTAC 2 cut(s) 2229, 2261
PstI CTGCAG 2 cut(s) 1195, 2038
PsuI RGATCY 1 cut(s) 2206
PsyI GACNNNGTC 3 cut(s) 131, 217, 328
PteI GCGCGC 2 cut(s) 417, 704
RruI TCGCGA 2 cut(s) 372, 666
RsaI GTAC 9 cut(s) 932, 1047, 1691, 1712, 1859, 1874, 1952, 2247, 2276
RsaNI GTAC 9 cut(s) 931, 1046, 1690, 1711, 1858, 1873, 1951, 2246, 2275
RseI CAYNNNNRTG 2 cut(s) 1036, 1909
SacI GAGCTC 2 cut(s) 153, 430
SacII CCGCGG 1 cut(s) 231
SalI GTCGAC 2 cut(s) 2533, 2582
Sau3AI GATC 8 cut(s) 410, 865, 1262, 1766, 2074, 2206, 2309, 2467
Sau96I GGNCC 4 cut(s) 1012, 1804, 2108, 2551
ScrFI CCNGG 8 cut(s) 60, 125, 1360, 1812, 1848, 1941, 2355, 2418
SduI GDGCHC 4 cut(s) 153, 430, 959, 2122
SfaNI GCATC 6 cut(s) 598, 1924, 2052, 2674, 2750, 2807
SfcI CTRYAG 4 cut(s) 940, 1191, 1332, 2034
Sfr274I CTCGAG 2 cut(s) 1049, 1989
Sfr303I CCGCGG 1 cut(s) 231
SgrBI CCGCGG 1 cut(s) 231
SinI GGWCC 4 cut(s) 1012, 1804, 2108, 2551
SlaI CTCGAG 2 cut(s) 1049, 1989
SmiMI CAYNNNNRTG 2 cut(s) 1036, 1909
SmlI CTYRAG 2 cut(s) 1049, 1989
SmoI CTYRAG 2 cut(s) 1049, 1989
SpeI ACTAGT 1 cut(s) 2303
SphI GCATGC 2 cut(s) 538, 1377
SspMI CTAG 6 cut(s) 1275, 1532, 1628, 2304, 2529, 2745
SstI GAGCTC 2 cut(s) 153, 430
StyD4I CCNGG 8 cut(s) 58, 123, 1358, 1810, 1846, 1939, 2353, 2416
StyI CCWWGG 2 cut(s) 525, 2547
TaaI ACNGT 5 cut(s) 1975, 2108, 2250, 2680, 2812
TaiI ACGT 5 cut(s) 218, 272, 293, 930, 2339
TaqII GACCGA 2 cut(s) 70, 1792
TatI WGTACW 5 cut(s) 930, 1710, 1857, 1872, 2274
TauI GCSGC 6 cut(s) 230, 233, 236, 482, 628, 631
TfiI GAWTC 7 cut(s) 444, 753, 1006, 1072, 2024, 2288, 2783
TscAI CASTG 5 cut(s) 494, 550, 796, 1043, 1182
TseFI GTSAC 4 cut(s) 139, 473, 2357, 2522
TseI GCWGC 7 cut(s) 97, 186, 493, 1145, 1190, 1535, 1665
Tsp45I GTSAC 4 cut(s) 139, 473, 2357, 2522
TspDTI ATGAA 9 cut(s) 401, 436, 756, 834, 1273, 2016, 2179, 2242, 2399
TspGWI ACGGA 4 cut(s) 265, 334, 418, 2658
TspRI CASTG 5 cut(s) 494, 550, 796, 1043, 1182
Tth111I GACNNNGTC 3 cut(s) 131, 217, 328
Van91I CCANNNNNTGG 1 cut(s) 130
VpaK11BI GGWCC 4 cut(s) 1012, 1804, 2108, 2551
VspI ATTAAT 2 cut(s) 848, 1305
XagI CCTNNNNNAGG 1 cut(s) 122
XapI RAATTY 5 cut(s) 1118, 1412, 1460, 1494, 2707
XceI RCATGY 4 cut(s) 538, 1249, 1377, 1764
XcmI CCANNNNNNNNNTGG 1 cut(s) 2573
XhoI CTCGAG 2 cut(s) 1049, 1989
XmiI GTMKAC 4 cut(s) 1638, 2511, 2534, 2583
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 6 cut(s) 1275, 1532, 1628, 2304, 2529, 2745
ZraI GACGTC 2 cut(s) 216, 270
Zsp2I ATGCAT 2 cut(s) 613, 2743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.