Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Reverse (-)

10034762 .. 10043181

Gene Structure 8420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1123400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5517 bp

ATGGATATCCGGTTTCCCTACTCGCCAGCCGAGGTGGCGAAGGTGAAATGCGTGCAGTTCGGCATCCTCAGCCCCGACGAAATCAGGCAAATGTCTGTCGTGGAGATTGAGCACAGTGAGACTATGTTGGGAGGCAAACCGAAGACGGCTGGTTTGAGTGATCCTCGGCTTGGTACAATTGATCGGAAGTTGAAATGTGAGACGTGTACAGCCAACATGGCTGAGTGCCCTGGCCATTTTGGGCACCTTGAGCTTGCAAAACCAATGTTTCACATCGGTTTCATGAAGATTGTGCTGTCCGTAATGCGATGCGTTTGCTTCAATTGCTCGAAAATTCTAGTCGATGAGGACGACCACAAGTTTAAGCAAGCTATGAGAATAAAGAATCCGAAAAGCAGGCTGAAAAAAATTTTGGATGCCTGCAAAAACAAAACCAAATGTGAAGGTGGTGATGAAATTGATGTCCAAGGCCAAGATACTGAAGAGCCTGTAAAGAAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCCAAGCTCTCTATTGAGGGTATGAAAATGAATGCAGAGTACAAAGCCCAAAGGAAGAAAAACGATGACCCGGAACAGCTTCCTGAACCCGTGGAAAGGAAACAGACCCTTACTGCAGAAAGGGTTCTTAGTGTTTTAAAGAGAATAAGTGATGAAGACTCCCAAATATTGGGATTAAACCCAAAGTACGCTCGGCCAGATTGGATGATTCTACAAGTTCTGCCTATACCTCCACAACCTGTGAGACCCTCTGTAATGATGGACACCTCATCCAGGAGTGAGGATGATTTGACTCATCAATTGGCGATGATAATTCGTCATAATGACAATCTCAGGCGGCAGGAACGAAATGGTTCTCCTGCTCATATCATTTCAGAGTTTGCACAACTTCTGCAATTCCACATTGCCACTTATTTTGACAATGAGTTGCCAGGACTACCAAGGGCTACGCAGAGATCAGGGAGGCCTATTAAATCAATTTGTAGTAGACTGAAGGCGAAAGAAGGGCGTATAAGAGGCAACTTAATGGGGAAACGTGTTGATTTTTCAGCTCGGACAGTGATTACTCCCGATCCAAACATAAACATTGATCAATTAGGAGTGCCGTGGAGTATTGCTTTGAATCTCACATACCCCGAGACTGTGACTCCATATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTATGGCCCCCATCCTCCGCCCGGTAAAACTGGTGCGAAGTATATAATAAGAGATGATGGGCAAAGGCTTGATCTTCGTTATTTGAAGAAAAGCATAAATTTTGAACTTCTACTTTTGTGGCTTCAGGTGGAACGTCATTTGAATGATGGAGACTTTGTCCTCTTTAATCGACAACCTAGTCTCCATAAAATGTCTATCATGGGCCACAGAATCAAGATCATGCCATACTCGACATTCCGATTGAATCTGTCTGTGACCTCGCCATATAATGCTGACTTTGACGGGGATGAAATGAACATGCATGTGCCCCAGTCCTTTGAAACCAGGGCCGAAGTTTTGGAGCTCATGATGGTGCCAAAATGCATCGTCTCACCTCAGGCTAATCGACCTGTCATGGGAATTGTGCAGGACACACTTTTAGGTTGTCGTAAGATCACGAAGAGGGATACCTTCATAGAGAAGGATGTTTTCATGAATATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTTCCTGCTCCTGCGATTTTGAAGCCCCGGCCACTTTGGACTGGAAAACAGGTGTTTAATCTTATAATACCAAAACAAATAAATCTCCAAAGAACTTCTGCATGGCACTCAGAATCAGAGAGTGGGAACATAACTCCAGGGGATACTCTTGTTAGAATAGAAAAGGGTGAATTGCTTTCTGGAACTCTCTGCAAGAAGGCCCTTGGGACTTCTACAGGAAGTCTTATTCATGTTATATGGGAAGAGGTTGGTCCAGATGCAGCCCGCAAGTTTCTGGGTCATACCCAATGGCTTGTAAATTACTGGCTGCTGCAGAATGCTTTTAGCATTGGAATTGGAGATACTATTGCTGATGCAGCCACAATGGAGAAAATCAATGAAACAATTTCCAAGGCGAAAAATGAGGTGAAAGATCTTATAAGTAAAGCCCAATCCAAGCAGCTGGAGGCTGAACCTGGACGAACCATGATGGACTCGTTTGAAAACAAAGTGAACCAGGTGTTGAATAGGGCTCGTGATGACGCTGGAAGTAGTGCTCAAAAGAGTTTAGATGAGAGCAACAATCTTAAGGCCATGGTTACAGCAGGGTCCAAAGGGAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAAAATGTTGAGGGTAAGCGAATCCCGTATGGGTTCATAGACCGGACGTTGCCCCACTTTACTAAAGATGATTATGGACCAGAAAGTCGTGGATTTGTAGAAAATTCATACCTGCGTGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGTGGTAGGGAAGGTCTTATAGATACTGCTGTTAAGACATCTGAGACTGGGTACATCCAAAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTGAAATATGATGGCACAGTACGTAATTCTTTAGGCGATGTCATTCAGTTTCTATATGGGGAAGATGGGATGGATGCTGTTTGGATAGAATCGCAAAAGCTGGATTCTTTGAAAATGAAAAAAACAGAATTTGATAGGACTTTTACTTATGAGTTGGATGATGAAAATTGGAATCCTGACTACATGTTGCACGAACATGTTGAGGATCTCAAAACGATTAGAGAGTTCCGTAATGTGTTTGATGCAGAAGTTCAGAAACTTGAAACAGATAGATTACAACTGGGAACAGAGATTGCAGTCACAGGGGATAATTCTTGGCCACTGCCTGTTAACTTGAAGAGGCTTATTTGGAATGCACAAAAAACCTTTAAGATTGACTTCCGAAGGACTTCTGATATGCACCCAATGGAAATTGTGGAAGCTATTGATAAGCTCCAGGAGAGACTGAAAGTTGTTCCTGGTGATGACCAGTTGAGTGTTGAAGCGCAGAAGAACGCAACGTTGTTTTTCAACATTTTACTTCGCAGCACTTTCGCAAGCAAACGGGTGTTGGATGAGTACAGGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAATCACGCTTCCTGCAATCACTTGTAGCACCAGGGGAAATGATTGGTTGCGTTGCTGCACAGTCTATTGGAGAGCCTGCCACTCAGATGACACTGAATACTTTCCACTATGCTGGTGTGAGTGCAAAGAATGTTACCCTAGGTGTCCCCAGGTTAAGGGAAATTATTAATGTTGCCAAGAGAATCAAAACGCCATCCCTCTCTGTCTACTTAAAGTCTGAAGCTAATAAAACGAAGGAGCGGGCCAAGAATGTGCAGTGTGCTTTGGAATACACCACTCTGCGAAGTGTAACTCAAGCTACAGAAGTATGGTACGATCCAGACCCCACAAGCACAGTAATTGAAGAGGATATAGATTTTGTGAAAGCTTACTATGAAATGCCAGATGAAGAGGTTAATCCTGATAAAATTTCACCTTGGTTGCTTCGCATTGAGTTGAATCGCGAAATGATGGTGGATAAGAAGTTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGACGATGATTTGACTTGTATCTTCAATGATGACAATGCTGAAAAATTGATCCTTCGTATCCGTATCATGAATGCTGAAGCTCCAAAAGGGGAGATGGTTGATGAATCTGCTGAAGATGATGTTTTTCTGAAGAAGATTGAGAGTAACATGCTGACAGAGATGGCTCTTCGTGGTATTCCAGATATTAACAAAGTGTTCATTAAACACGGTAAAGTTAACAAGTTCGATCAGAATGAAGGATTTAAAGCGGAGCAAGAGTGGATGCTGGATACCGAAGGGGTTAATCTCTTAGCTGTTTTGTGCCACGATGATGTTGATGCTAGGCGCACAACTAGTAATCATTTGATTGAAATTCTGGAAATTCTCGGAATTGAGGCAGTTCGTCGGTCATTGTTGGATGAGTTGAGGGTTGTTATATCGTTTGATGGGTCCTATGTTAATTATCGGCATCTCGCTATCCTTTGTGACACGATGACCTACCGTGGGCACTTAATGGCAATAACTCGTCATGGCATTAATAGAAATGACACTGGTCCTATGATGAGGTGCTCCTTTGAAGAGACTGTTGATATTCTTCTTGATGCTGCAGTGTTCGCAGAGACGGATTATTTAAGAGGTGTGACAGAAAATATAATGTTAGGACAACTTGCACCAATTGGAACAGGAGATTGTGCCCTGTATCTCAATGATGAGATGCTGAAGAATGCCATTGAACTGCAGCTGCCCAGTTACATGGATGGTCTTGATTTTGGTATGACACCGTCTCGTTCTCCAGTATCAGGAACACCATATCACGAGGGCATGATGTCCCCTAATTATTTGTTGAGCCCAAATCTTCGGTTGTCGCCAATTTCAGATGCTCAGTTTTCTCCATATGTTGGAGGAATGGCATTCTCTCCTACTTCCTCTCCAGGCTACAGCCCATCATCCCCTGGATACAGCCCTTCCTCGCCTGGATACAGCCCCACTTCTCCTGGGTACAGTCCCACCTCACCAGGGTATAGCCCCACTTCTCCTGGGTACAGTCCCACCTCTCCGACATATAGTCCTAGTTCTCCTGGCTATAGTCCCACCAGTCCAGCTTACTCTCCTACGAGTCCATCATACTCACCAACCTCTCCCAGCTACAGCCCCACCTCACCAAGCTACAGTCCCACCTCACCAAGCTACAGCCCCACTTCCCCTGCTTACAGCCCCACTTCCCCCGCTTACAGCCCCACTTCCCCTGCATATAGCCCCACTTCCCCATCTTACAGTCCCACGTCTCCTTCATACAGCCCAACTTCGCCCTCCTACAGCCCAACTTCGCCCTCCTACAGCCCAACTTCTCCCTCCTACAGCCCCACATCTCCATCCTACAGCCCTACCTCGCCAGCCTACAGCCCAACTTCTCCTGGTTACAGCCCAACCTCTCCCAGTTATAGTCCAACCTCGCCCAGTTACAGCCCTACATCTCCAAGCTATAATCCTCAGTCTGCCAAGTACAGTCCGTCGTCAGCTTACTCTCCAAGCAGTCCAAGATTGTCACCATCTAGTCCATACAGTCCTACCTCTCCGAACTACAGCCCAACCTCACCATCATATTCACCCACATCTCCATCGTACTCTCCCTCAAGCCCCACATACAGTCCGGGCAGCCCATTGACTTCTGGAGTGAGCCCAGACTATAGCCCGAGTTCCCCTCAGTATAGTCCAAGTGCTGGATACTCACCTAGTCAACCAGCTCGCCTGCTACCATCGGAGAACAGACTAATTGGAACTGAGAAGTCACGTTCAAGCTGGCCGCTTCATGGCAGGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1839

Amino Acids

205.0

Weight (kDa)

5.84

Isoelectric Point (pI)

63.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	14 - 350	1.4e-114	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	352 - 521	6e-69	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	524 - 688	1.5e-51	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	714 - 819	3e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	826 - 1416	3.1e-104	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	892 - 1076	2.3e-68	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1161 - 1295	6e-53	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1784, 2138
AasI	GACNNNNNNGTC	2 cut(s)	1386, 2442
Acc36I	ACCTGC	1 cut(s)	2478
AccB1I	GGYRCC	2 cut(s)	243, 1561
AccB7I	CCANNNNNTGG	3 cut(s)	694, 4592, 5414
AccBSI	CCGCTC	1 cut(s)	3448
AccI	GTMKAC	2 cut(s)	1012, 3414
AccII	CGCG	1 cut(s)	3651
AciI	CCGC	7 cut(s)	862, 1226, 1984, 3448, 3962, 4920, 5498
AclI	AACGTT	1 cut(s)	3104
AclWI	GGATC	5 cut(s)	155, 1091, 2817, 3518, 3757
AcoI	YGGCCR	5 cut(s)	232, 719, 1748, 2921, 5495
AcsI	RAATTY	9 cut(s)	333, 408, 1306, 2461, 2732, 3615, 3710, 4065, 4074
AcuI	CTGAAG	8 cut(s)	501, 1037, 1316, 3447, 3810, 3846, 3863, 4433
AflII	CTTAAG	1 cut(s)	2285
AflIII	ACRYGT	4 cut(s)	203, 1060, 2787, 2800
AhdI	GACNNNNNGTC	1 cut(s)	4758
AhlI	ACTAGT	1 cut(s)	4046
AjiI	CACGTC	2 cut(s)	204, 4977
AjuI	GAANNNNNNNTTGG	2 cut(s)	395, 427
Alw21I	GWGCWC	5 cut(s)	114, 520, 1554, 2257, 4265
AlwI	GGATC	5 cut(s)	155, 1091, 2817, 3518, 3757
Ama87I	CYCGRG	2 cut(s)	1160, 5386
ApoI	RAATTY	9 cut(s)	333, 408, 1306, 2461, 2732, 3615, 3710, 4065, 4074
ArsI	GACNNNNNNTTYG	2 cut(s)	136, 168
AseI	ATTAAT	2 cut(s)	3375, 4230
Asp700I	GAANNNNTTC	5 cut(s)	603, 648, 877, 2992, 3308
AspA2I	CCTAGG	1 cut(s)	3346
AspLEI	GCGC	2 cut(s)	3091, 4041
AsuC2I	CCSGG	4 cut(s)	596, 1230, 1747, 5346
AvaI	CYCGRG	2 cut(s)	1160, 5386
AvaII	GGWCC	5 cut(s)	1970, 2307, 2435, 4143, 4247
AvrII	CCTAGG	1 cut(s)	3346
AxyI	CCTNAGG	1 cut(s)	1584
BaeGI	GKGCMC	5 cut(s)	230, 246, 1518, 4203, 4390
BaeI	ACNNNNGTAYC	4 cut(s)	1854, 1887, 2526, 2559
BalI	TGGCCA	2 cut(s)	234, 2923
BanI	GGYRCC	2 cut(s)	243, 1561
BanII	GRGCYC	4 cut(s)	1554, 2233, 4544, 5375
BauI	CACGAG	3 cut(s)	2232, 3174, 4508
BbsI	GAAGAC	2 cut(s)	149, 687
Bbv12I	GWGCWC	5 cut(s)	114, 520, 1554, 2257, 4265
BbvCI	CCTCAGC	1 cut(s)	68
BceAI	ACGGC	2 cut(s)	162, 1114
BcgI	CGANNNNNNTGC	4 cut(s)	297, 331, 3118, 3152
BciVI	GTATCC	7 cut(s)	1648, 1855, 3782, 3976, 4643, 4664, 5411
BclI	TGATCA	1 cut(s)	1114
BcnI	CCSGG	4 cut(s)	596, 1230, 1747, 5346
BcuI	ACTAGT	1 cut(s)	4046
BfaI	CTAG	8 cut(s)	338, 1386, 3347, 4035, 4047, 4764, 5247, 5427
BfrI	CTTAAG	1 cut(s)	2285
BfuAI	ACCTGC	1 cut(s)	2478
BfuI	GTATCC	7 cut(s)	1648, 1855, 3782, 3976, 4643, 4664, 5411
BglI	GCCNNNNNGGC	2 cut(s)	35, 218
BglII	AGATCT	1 cut(s)	2131
BlnI	CCTAGG	1 cut(s)	3346
BlpI	GCTNAGC	1 cut(s)	519
Bme18I	GGWCC	5 cut(s)	1970, 2307, 2435, 4143, 4247
BmeRI	GACNNNNNGTC	1 cut(s)	4758
BmeT110I	CYCGRG	2 cut(s)	1160, 5386
BmgBI	CACGTC	2 cut(s)	204, 4977
BmiI	GGNNCC	5 cut(s)	245, 1216, 1563, 2308, 4144
BmrI	ACTGGG	6 cut(s)	1513, 2568, 2894, 4434, 5124, 5145
BmuI	ACTGGG	6 cut(s)	1513, 2568, 2894, 4434, 5124, 5145
BoxI	GACNNNNGTC	1 cut(s)	4245
BpiI	GAAGAC	2 cut(s)	149, 687
BplI	GAGNNNNNCTC	4 cut(s)	148, 180, 5380, 5412
BpmI	CTGGAG	6 cut(s)	1839, 2183, 3023, 4470, 4608, 5385
Bpu10I	CCTNAGC	1 cut(s)	68
Bpu1102I	GCTNAGC	1 cut(s)	519
BpuEI	CTTGAG	3 cut(s)	269, 3486, 5311
BpuMI	CCSGG	4 cut(s)	596, 1230, 1747, 5346
BsaAI	YACGTR	1 cut(s)	2627
BsaI	GGTCTC	1 cut(s)	763
BsaWI	WCCGGW	2 cut(s)	9, 2400
Bse21I	CCTNAGG	1 cut(s)	1584
Bse3DI	GCAATG	2 cut(s)	927, 3687
BseMI	GCAATG	2 cut(s)	927, 3687
BseSI	GKGCMC	5 cut(s)	230, 246, 1518, 4203, 4390
BseYI	CCCAGC	1 cut(s)	4835
BsgI	GTGCAG	4 cut(s)	74, 1634, 3249, 3482
Bsh1236I	CGCG	1 cut(s)	3651
BshNI	GGYRCC	2 cut(s)	243, 1561
BsiHKAI	GWGCWC	5 cut(s)	114, 520, 1554, 2257, 4265
BsiHKCI	CYCGRG	2 cut(s)	1160, 5386
BsiSI	CCGG	6 cut(s)	10, 596, 1230, 1747, 2401, 5345
BslFI	GGGAC	9 cut(s)	1939, 2367, 3338, 4507, 4683, 4725, 4767, 4851, 4956
BsmBI	CGTCTC	5 cut(s)	194, 1582, 4307, 4482, 4983
BsmFI	GGGAC	9 cut(s)	1939, 2367, 3338, 4507, 4683, 4725, 4767, 4851, 4956
BsmI	GAATGC	6 cut(s)	562, 2041, 2962, 3790, 4423, 4604
Bso31I	GGTCTC	1 cut(s)	763
BsoBI	CYCGRG	2 cut(s)	1160, 5386
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	1 cut(s)	519
Bsp19I	CCATGG	1 cut(s)	2292
Bsp68I	TCGCGA	1 cut(s)	3651
BspACI	CCGC	7 cut(s)	862, 1226, 1984, 3448, 3962, 4920, 5498
BspFNI	CGCG	1 cut(s)	3651
BspHI	TCATGA	4 cut(s)	282, 1554, 1680, 3780
BspLI	GGNNCC	5 cut(s)	245, 1216, 1563, 2308, 4144
BspMAI	CTGCAG	4 cut(s)	643, 2034, 4303, 4434
BspMI	ACCTGC	1 cut(s)	2478
BspPI	GGATC	5 cut(s)	155, 1091, 2817, 3518, 3757
BspQI	GCTCTTC	2 cut(s)	477, 3885
BspT107I	GGYRCC	2 cut(s)	243, 1561
BspTI	CTTAAG	1 cut(s)	2285
BspTNI	GGTCTC	1 cut(s)	763
BsrBI	CCGCTC	1 cut(s)	3448
BsrDI	GCAATG	2 cut(s)	927, 3687
BsrGI	TGTACA	1 cut(s)	206
BssSI	CACGAG	3 cut(s)	2232, 3174, 4508
BssT1I	CCWWGG	7 cut(s)	466, 965, 1921, 2109, 2292, 3346, 3623
Bst2BI	CACGAG	3 cut(s)	2232, 3174, 4508
Bst6I	CTCTTC	8 cut(s)	477, 1643, 1956, 2936, 3546, 3591, 3885, 4266
BstAFI	CTTAAG	1 cut(s)	2285
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	1 cut(s)	2627
BstDSI	CCRYGG	4 cut(s)	615, 1130, 2292, 4195
BstENI	CCTNNNNNAGG	1 cut(s)	796
BstFNI	CGCG	1 cut(s)	3651
BstHHI	GCGC	2 cut(s)	3091, 4041
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2075, 3689, 4307
BstNSI	RCATGY	5 cut(s)	1510, 1514, 2791, 2804, 3865
BstPAI	GACNNNNGTC	1 cut(s)	4245
BstSLI	GKGCMC	5 cut(s)	230, 246, 1518, 4203, 4390
BstSNI	TACGTA	1 cut(s)	2627
BstUI	CGCG	1 cut(s)	3651
BstV2I	GAAGAC	2 cut(s)	149, 687
BstX2I	RGATCY	2 cut(s)	2131, 2809
BstXI	CCANNNNNNTGG	3 cut(s)	2161, 3320, 4447
BstYI	RGATCY	2 cut(s)	2131, 2809
Bsu36I	CCTNAGG	1 cut(s)	1584
BsuI	GTATCC	7 cut(s)	1648, 1855, 3782, 3976, 4643, 4664, 5411
BtgI	CCRYGG	4 cut(s)	615, 1130, 2292, 4195
BtgZI	GCGATG	3 cut(s)	322, 845, 2655
BtrI	CACGTC	2 cut(s)	204, 4977
BtsI	GCAGTG	3 cut(s)	2924, 3470, 4308
BtsIMutI	CAGTG	7 cut(s)	121, 1089, 2924, 3299, 3470, 4242, 4308
BtuMI	TCGCGA	1 cut(s)	3651
BveI	ACCTGC	1 cut(s)	2478
CciI	TCATGA	4 cut(s)	282, 1554, 1680, 3780
CfoI	GCGC	2 cut(s)	3091, 4041
CseI	GACGC	1 cut(s)	2249
CsiI	ACCWGGT	1 cut(s)	2214
CspCI	CAANNNNNGTGG	2 cut(s)	922, 957
DraI	TTTAAA	2 cut(s)	663, 3958
DrdI	GACNNNNNNGTC	2 cut(s)	1386, 2442
DriI	GACNNNNNGTC	1 cut(s)	4758
DseDI	GACNNNNNNGTC	2 cut(s)	1386, 2442
EaeI	YGGCCR	5 cut(s)	232, 719, 1748, 2921, 5495
Eam1104I	CTCTTC	8 cut(s)	477, 1643, 1956, 2936, 3546, 3591, 3885, 4266
Eam1105I	GACNNNNNGTC	1 cut(s)	4758
EarI	CTCTTC	8 cut(s)	477, 1643, 1956, 2936, 3546, 3591, 3885, 4266
EciI	GGCGGA	1 cut(s)	1215
Ecl136II	GAGCTC	1 cut(s)	1552
Eco105I	TACGTA	1 cut(s)	2627
Eco130I	CCWWGG	7 cut(s)	466, 965, 1921, 2109, 2292, 3346, 3623
Eco147I	AGGCCT	1 cut(s)	991
Eco24I	GRGCYC	4 cut(s)	1554, 2233, 4544, 5375
Eco31I	GGTCTC	1 cut(s)	763
Eco32I	GATATC	1 cut(s)	7
Eco47I	GGWCC	5 cut(s)	1970, 2307, 2435, 4143, 4247
Eco53kI	GAGCTC	1 cut(s)	1552
Eco57I	CTGAAG	8 cut(s)	501, 1037, 1316, 3447, 3810, 3846, 3863, 4433
Eco81I	CCTNAGG	1 cut(s)	1584
Eco88I	CYCGRG	2 cut(s)	1160, 5386
EcoICRI	GAGCTC	1 cut(s)	1552
EcoNI	CCTNNNNNAGG	1 cut(s)	796
EcoO109I	RGGNCCY	2 cut(s)	1918, 4143
EcoRV	GATATC	1 cut(s)	7
EcoT14I	CCWWGG	7 cut(s)	466, 965, 1921, 2109, 2292, 3346, 3623
EcoT22I	ATGCAT	2 cut(s)	1512, 1574
EcoT38I	GRGCYC	4 cut(s)	1554, 2233, 4544, 5375
ErhI	CCWWGG	7 cut(s)	466, 965, 1921, 2109, 2292, 3346, 3623
Esp3I	CGTCTC	5 cut(s)	194, 1582, 4307, 4482, 4983
FalI	AAGNNNNNCTT	2 cut(s)	2966, 2998
FaqI	GGGAC	9 cut(s)	1939, 2367, 3338, 4507, 4683, 4725, 4767, 4851, 4956
FauI	CCCGC	3 cut(s)	1991, 3441, 4927
FauNDI	CATATG	1 cut(s)	4588
FbaI	TGATCA	1 cut(s)	1114
FblI	GTMKAC	2 cut(s)	1012, 3414
FriOI	GRGCYC	4 cut(s)	1554, 2233, 4544, 5375
FspBI	CTAG	8 cut(s)	338, 1386, 3347, 4035, 4047, 4764, 5247, 5427
GlaI	GCGC	2 cut(s)	3090, 4040
GsaI	CCCAGC	1 cut(s)	4839
GsuI	CTGGAG	6 cut(s)	1839, 2183, 3023, 4470, 4608, 5385
HapII	CCGG	6 cut(s)	10, 596, 1230, 1747, 2401, 5345
HgaI	GACGC	1 cut(s)	2249
HhaI	GCGC	2 cut(s)	3091, 4041
Hin6I	GCGC	2 cut(s)	3089, 4039
HinP1I	GCGC	2 cut(s)	3089, 4039
HincII	GTYRAC	3 cut(s)	2935, 3931, 5432
HindII	GTYRAC	3 cut(s)	2935, 3931, 5432
HindIII	AAGCTT	1 cut(s)	3573
HpaI	GTTAAC	2 cut(s)	2935, 3931
HpaII	CCGG	6 cut(s)	10, 596, 1230, 1747, 2401, 5345
Hpy166II	GTNNAC	8 cut(s)	207, 1013, 2212, 2477, 2935, 3415, 3931, 5432
Hpy8I	GTNNAC	8 cut(s)	207, 1013, 2212, 2477, 2935, 3415, 3931, 5432
Hpy99I	CGWCG	3 cut(s)	80, 4101, 5209
HpyCH4IV	ACGT	8 cut(s)	203, 1060, 1342, 2405, 2626, 3104, 4976, 5485
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2075, 3689, 4307
HpySE526I	ACGT	8 cut(s)	203, 1060, 1342, 2405, 2626, 3104, 4976, 5485
HspAI	GCGC	2 cut(s)	3089, 4039
Ksp22I	TGATCA	1 cut(s)	1114
KspAI	GTTAAC	2 cut(s)	2935, 3931
LguI	GCTCTTC	2 cut(s)	477, 3885
LmnI	GCTCC	8 cut(s)	1198, 1549, 1732, 3042, 3445, 3799, 3964, 4268
MabI	ACCWGGT	1 cut(s)	2214
MaeI	CTAG	8 cut(s)	338, 1386, 3347, 4035, 4047, 4764, 5247, 5427
MaeII	ACGT	8 cut(s)	203, 1060, 1342, 2405, 2626, 3104, 4976, 5485
MbiI	CCGCTC	1 cut(s)	3448
MfeI	CAATTG	4 cut(s)	177, 322, 824, 4368
MflI	RGATCY	2 cut(s)	2131, 2809
MlsI	TGGCCA	2 cut(s)	234, 2923
MluNI	TGGCCA	2 cut(s)	234, 2923
MlyI	GAGTC	5 cut(s)	677, 811, 1165, 2186, 4819
MmeI	TCCRAC	6 cut(s)	2739, 3135, 4089, 4573, 4775, 5165
Mox20I	TGGCCA	2 cut(s)	234, 2923
Mph1103I	ATGCAT	2 cut(s)	1512, 1574
MroXI	GAANNNNTTC	5 cut(s)	603, 648, 877, 2992, 3308
MscI	TGGCCA	2 cut(s)	234, 2923
MslI	CAYNNNNRTG	7 cut(s)	1350, 1511, 1559, 2799, 3187, 3293, 4023
Msp20I	TGGCCA	2 cut(s)	234, 2923
MspA1I	CMGCKG	2 cut(s)	2161, 4435
MspCI	CTTAAG	1 cut(s)	2285
MspI	CCGG	6 cut(s)	10, 596, 1230, 1747, 2401, 5345
MunI	CAATTG	4 cut(s)	177, 322, 824, 4368
Mva1269I	GAATGC	6 cut(s)	562, 2041, 2962, 3790, 4423, 4604
MvnI	CGCG	1 cut(s)	3651
MwoI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2075, 3689, 4307
NciI	CCSGG	4 cut(s)	596, 1230, 1747, 5346
NcoI	CCATGG	1 cut(s)	2292
NdeI	CATATG	1 cut(s)	4588
NlaIV	GGNNCC	5 cut(s)	245, 1216, 1563, 2308, 4144
NmeAIII	GCCGAG	3 cut(s)	55, 145, 697
NmuCI	GTSAC	7 cut(s)	1168, 1462, 2902, 4178, 4333, 5238, 5481
NruI	TCGCGA	1 cut(s)	3651
NsiI	ATGCAT	2 cut(s)	1512, 1574
NspI	RCATGY	5 cut(s)	1510, 1514, 2791, 2804, 3865
PagI	TCATGA	4 cut(s)	282, 1554, 1680, 3780
PceI	AGGCCT	1 cut(s)	991
PciI	ACATGT	2 cut(s)	2787, 2800
PciSI	GCTCTTC	2 cut(s)	477, 3885
PcsI	WCGNNNNNNNCGW	1 cut(s)	348
PctI	GAATGC	6 cut(s)	562, 2041, 2962, 3790, 4423, 4604
PdmI	GAANNNNTTC	5 cut(s)	603, 648, 877, 2992, 3308
PflFI	GACNNNGTC	2 cut(s)	1364, 4474
PflMI	CCANNNNNTGG	3 cut(s)	694, 4592, 5414
PfoI	TCCNGGA	2 cut(s)	797, 3039
PleI	GAGTC	5 cut(s)	677, 811, 1165, 2186, 4818
PpsI	GAGTC	5 cut(s)	677, 811, 1165, 2186, 4818
Ppu21I	YACGTR	1 cut(s)	2627
PpuMI	RGGWCCY	1 cut(s)	4143
PscI	ACATGT	2 cut(s)	2787, 2800
PshAI	GACNNNNGTC	1 cut(s)	4245
PshBI	ATTAAT	2 cut(s)	3375, 4230
PsiI	TTATAA	2 cut(s)	1784, 2138
Psp124BI	GAGCTC	1 cut(s)	1554
Psp1406I	AACGTT	1 cut(s)	3104
Psp5II	RGGWCCY	1 cut(s)	4143
PspFI	CCCAGC	1 cut(s)	4835
PspN4I	GGNNCC	5 cut(s)	245, 1216, 1563, 2308, 4144
PspPPI	RGGWCCY	1 cut(s)	4143
PstI	CTGCAG	4 cut(s)	643, 2034, 4303, 4434
PsuI	RGATCY	2 cut(s)	2131, 2809
PsyI	GACNNNGTC	2 cut(s)	1364, 4474
PvuII	CAGCTG	2 cut(s)	2161, 4435
RruI	TCGCGA	1 cut(s)	3651
RseI	CAYNNNNRTG	7 cut(s)	1350, 1511, 1559, 2799, 3187, 3293, 4023
SacI	GAGCTC	1 cut(s)	1554
SapI	GCTCTTC	2 cut(s)	477, 3885
SchI	GAGTC	5 cut(s)	677, 811, 1165, 2186, 4819
SexAI	ACCWGGT	1 cut(s)	2214
SinI	GGWCC	5 cut(s)	1970, 2307, 2435, 4143, 4247
SmiMI	CAYNNNNRTG	7 cut(s)	1350, 1511, 1559, 2799, 3187, 3293, 4023
SmlI	CTYRAG	4 cut(s)	248, 2285, 3501, 5326
SmoI	CTYRAG	4 cut(s)	248, 2285, 3501, 5326
SnaBI	TACGTA	1 cut(s)	2627
SpeI	ACTAGT	1 cut(s)	4046
SseBI	AGGCCT	1 cut(s)	991
SsiI	CCGC	7 cut(s)	862, 1226, 1984, 3448, 3962, 4920, 5498
SspI	AATATT	2 cut(s)	693, 1183
SspMI	CTAG	8 cut(s)	338, 1386, 3347, 4035, 4047, 4764, 5247, 5427
SstI	GAGCTC	1 cut(s)	1554
StuI	AGGCCT	1 cut(s)	991
StyI	CCWWGG	7 cut(s)	466, 965, 1921, 2109, 2292, 3346, 3623
TaiI	ACGT	8 cut(s)	206, 1063, 1345, 2408, 2629, 3107, 4979, 5488
TaqI	TCGA	6 cut(s)	329, 342, 1378, 1439, 1594, 3939
TaqII	GACCGA	1 cut(s)	4089
TatI	WGTACW	4 cut(s)	206, 564, 3162, 5196
TauI	GCSGC	2 cut(s)	865, 5500
TscAI	CASTG	7 cut(s)	121, 1089, 2931, 3306, 3470, 4249, 4308
TseFI	GTSAC	7 cut(s)	1168, 1462, 2902, 4178, 4333, 5238, 5481
Tsp45I	GTSAC	7 cut(s)	1168, 1462, 2902, 4178, 4333, 5238, 5481
TspGWI	ACGGA	5 cut(s)	289, 2822, 3764, 4331, 5193
TspRI	CASTG	7 cut(s)	121, 1089, 2931, 3306, 3470, 4249, 4308
Tth111I	GACNNNGTC	2 cut(s)	1364, 4474
Van91I	CCANNNNNTGG	3 cut(s)	694, 4592, 5414
Vha464I	CTTAAG	1 cut(s)	2285
VpaK11BI	GGWCC	5 cut(s)	1970, 2307, 2435, 4143, 4247
VspI	ATTAAT	2 cut(s)	3375, 4230
XagI	CCTNNNNNAGG	1 cut(s)	796
XapI	RAATTY	9 cut(s)	333, 408, 1306, 2461, 2732, 3615, 3710, 4065, 4074
XceI	RCATGY	5 cut(s)	1510, 1514, 2791, 2804, 3865
XmaJI	CCTAGG	1 cut(s)	3346
XmiI	GTMKAC	2 cut(s)	1012, 3414
XmnI	GAANNNNTTC	5 cut(s)	603, 648, 877, 2992, 3308
XspI	CTAG	8 cut(s)	338, 1386, 3347, 4035, 4047, 4764, 5247, 5427
Zsp2I	ATGCAT	2 cut(s)	1512, 1574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.