Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Reverse (-)

10941944 .. 10950047

Gene Structure 8104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG124000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5319 bp

ATGGATTTCCGGTTCCCATACTCGCCTCTGGAGGTTGCGACGCCGAGCTCCGTCCAGTTCGGCATCCTCAGCCCCGGCGAGATTAGGCAAATGTCGGTGCTGGAGGTCGAGGATGGCCGTTCAATGCTGAACGGGAAGCCGGTGAGAGGCGGCTTGAGTGACCCTCGGCTTGGTCCGATTGACAGGCACAATAGGTGTGAGACTTGTGCCAATAACTTGAGCGAGTGCCCGGGCCATTTCGGCCACATTGAGCTCGCCAAGCCTGTGTTCCACTTCGGGTTTATGAAGACTCTGCTGTCTGTAATGCGCTGCGTTTGCTTCAATTGCTCCAAACTTCTACTCCATGAGGAAGATGTTAAGTTTAAACAAGCTTTGAGAATCAAGAAGCCTAAAGATAGGCTAAAAAAGTTTGTGGATGCATGCAAAAACAAGACCAAGTGTGAAGGTGGTGATGAAATTGATCTGCAGGGCTCAAAATCCGGTCAACATGTAAAGCAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCCAAGTTCTCTATGGCTGGTTTGGAACTGGTTGCAGAGTACAAATCACAAAGGAAACAATATGATGAGGAACAGCTTCCTGAACCAGTGGAAAGGAAACAAATCCTTACTGCAGAAAAGGTTCTTAGCTTATTAAAGAGGATAAGTGATGAGGACTGCCAATTCTTGGGACTGAACCCTGAATATGCTCGACCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACCAGCTGTGAGACCCCCGGTGATGATGAGCAGCTCTGCCATGAGTGAGGATGATTTAACACATCAGTTGTCCATGATTATTCGCCACAACGAGAATTTGAGGAAACAGGAAAAACATGGTTCAGCTGCTCATATCATTTCACAGTTTACACAAATCTTGCAATACTACATATCCACATATTTTGACAATAGCTTGCCTGGACTACCAAAGGCTGCTCATGGATCGACAAAGAGGCCCATTAAATCAATATGTTGTAGGCTTAAGGCAAAGCATGGCCGGATTAGAGGAAACTTGATGGGGAAGAGAGTCGATTTCTCAGCACGGACAGTTATTACACCAGATCCGAACCTTAGCCTTGATCAATTAGGAGTACCATGGAGCATTGCTATGAATCTCACATACACAGAGACTGTGACTCCATTTAATAAAGAAGGGTTGAAGGAGCTTGTTGAGTATGGGCCGCATCCCCCACCTGGACTAACTGGTGCAAAGTACATAATACGAGATGATGGCCAAAGACTCGATCTTCGTTACATGAAGAAAGATAGTGATCATCATTTAGAGCTTGGTTATAAGGTGGAGCGTCATCTAAAGGATGGAGACATTGTCCTCTTAAATCGTCAGCCTAGTCTCCATAAAATGTCATTCATGGGGCATAGAATCAAGATCATGCCATACTCAACTCTTCGCTTGAACTTATCTGTCACTTCACCTTACAATGCTGATTTTGATGGGGATGAAATGAACGTCCATGTTCCTCAGTCATTTGAAACTAGGGCAGAAGCTCTGGAGCTCATGATGGTGCCCAAATGCATTGTTTCCCCTCAGTCGAACCGGCCTGTAATGGGAATAGTCCAAGATACACTGTTAGGTGCTCGAAAAATCACAAAAAGGGATACCTTCATAGAGAAGGACGTTTTTATGAACATCTTGATGTGGTGGGAGGATTTTGACGGGAAAGTGCCTGCTCCTGCAATATTGAAGCCACGACCTCTTTGGACAGGGAAACAGGTATTTAATCTTATAATACCGAAACAGATAAATCTCCAGAGAACTTCAGCGTGGCACTCTGAATCAGAAAGTGGTAACATTACCCCTGGTGATACTTTCGTTAGAATAGAAAAAGGGGAGTTACTTTCTGGAACTCTCTGCAAAAGGGCACTTGGGACTTCTACAGGAAGTCTTATACATGTCATATGGGAAGAGGTTGGTCCAGATGCAGCCTCCAAATTCCTTAGTAATACCCAGTGGCTTGTGAATTACTGGCTGTTGCAGGATGGTTTTAGCATTGGAATTGGAGATACAATTGCTGATGCAGCAACAATGGAAAAGATCAATGTAACTATTTCCAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAGGCAGCTGGAGGCTGAACCAGGACGAACTATGATGGAATCCTTTGAAAACAAAGTGAACCAGGTGTTGAATAGGGCTCGTGATGATGCTGGAAGTAGTGCCCAGAAGAGTTTATCTGAGAGTAACAATCTCAAGGCCATGGTCACAGCCGGATCGAAGGGAAGTTTTATCAATATATCGCAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAGCGAATTCCGTTTGGGTTCACAGACCGGACTTTGCCCCATTTTACGAAGGACGATTATGGACCGGAAAGTCGTGGCTTTGTAGAGAATTCCTACCTGCGTGGATTAACTCCACAGGAGTTTTATTTTCATGCTATGGGTGGTAGGGAAGGCATTATTGATACTGCAGTTAAGACATCTGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTTAAATATGATGGGACTGTCAGAAATTCAGTGGGGGATGTAATACAGTTTCTCTATGGGGAAGATGGAATGGACGCTGTTTGGTTAGAATCACAAAAGCTGGATTCTTTAAAGATGAAAAAATCAGAATTTAACAAGACTTTCACTGTTGAGTTTGATGATAGAAATTGGAATCCGGACTATATGTTGCCTGAATATGTTGAGGATCTAAAAACTATTGGAGAGTTTCGCTGGGCATTGGACGCTGAAGTTTCGAAACTTGAAGCTGATAGGATTCAACTTGGCACAGAGATTGCTGTCACAGGTGATAATACTTGGCCGTTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTCAAAATTGACTTCCGAAGGACTTCTGATATGCAACCAATGGAAATTATAGAAGCTATTGATAATCTTCAAGAGAGGCTGAAGGTTGTTCCTGGTGATGACTTGTTAAGTGTTGAAGCTCAAAATAATGCTACACTCTTTTTCAATATTTTGCTTCGCAGCACTTTTGCTAGCAAAAGGGTGCTAAAAGAGTACAAGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGGCACGCTTCCTGCAATCACTTGTAGCACCTGGGGAAATGATCGGTATTGTTGCTGCACAATCTATTGGCGAGCCTGCCACTCAGATGACTCTCAATACCTTCCACTATGCCGGTGTAAGTGCAAAGAATGTTACTCTAGGTGTTCCAAGGTTGAAGGAAATTATTGATGTATCTAAGCGTATCAAAACACCATCTCTCTCTATCTACTTGAAGCCTGAAGCTAATAGAACCAAGGAGAAGGCCAAGAATGTCCAGTGTGCTTTGGAATACACTAACCTCGGAAGTGTTACTCAAGCTACAGAATTGTGGTATGATCCGGACCCTATGAGCACAATAATTGAAGAGGATCTTGAATTTGTCCAATCTTACTATGAAATGCCAGATGAAGAGGTTAGCCCTGAAAAAATATCACCTTGGTTGCTTCGCATAGAGATGAATCGTGAAATGATGGTGGATAAGAAACTGAGTATGGCTGACATTGCTGAGAAGATTAATTGTGAATATAGTGGTAAAATACAGTGTATCTTTAATGATGACAATTCTGAGAAGTTGATACTACGAATCCGTCTCATGAATGAAGAAGCTCCACATGAGGAAGTGAATGATCAATCTGAAGATGATGTTTTCCTGAAGATGATTGAGAGTAACATGCTTACAAAAATGGCTCTTCAAGGGATCCCAGAGATTAAAAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGGTTTATTGCGGAGCAAGAGTGGATGCTGGATACTGAAGGTGTTAATCTCTTAGCTGTCATGTGCCATGAGGATGTTGATGCCAGGAGAACGACAAGCAATCATTTGTATGAAATTATGGAAGTTCTTGGGATTGAGGCAGCTCGACGGGCATTGCTGGATGAGTTGCGTAATGTTATTTCATTTGATGGATCCTATGTAAATTACCGGCACCTGGCCATTCTTTGTGATACCATGACCTATCGTGGCTACTTGATGGCCATCACTCGACATGGTATCAATCGAAAGGACACTGGTCCTATGATGCGATGCTCGTTTGAACAAACAGTGGACATGCTGCTGGATGCTTCAGTTTTTGCAGAAACAGATCATCTGAGGGGTGTAACAGAAAACATAATACTAGGCAAGCTGGCACCAATTGGCACTGGAGACTGTGCCCTGTATCTGAATGAAGAGATGTTACAAAATGCTATGGAAGTGCAGCTACCTGGTTATATGGATGGCCTTGATGTTGGCATGACACCATCCCATTCTCCATACCTTGAGAGCATACCGTCTCCTAGTTATTTGCGGAGTCCCAGTAATCCTCTTTCACCAATGTTGGATGCAGCACAGTTTTCTCCATACGTTAGGGGAATTCCCTTTTCTCCTACTTCATCTCCAGGCAACATCCCATCATCTCTTGGCTACTATCTGCCATCCTTTGTATACAGTCCCACCTCCCCATACTATAGCCCCACGTCACCAGGTTATAGTCCAATCAGTCCCCCCTATTCTCCTTCACGTCCATCTTTCACATCACCAGGTTATACTCCAACCAGTCCTCCCTATTCTCCTTCACGTCCATCTTTCACATCACCAGGTTATAGTCCAACCAGTCCTGTCTATTCTCCTACAAGTCCATATTACTCACCGACCTCACCCAACTATAGCCCTATCTCTCCAACATCAACTCCTTATAGCCCAACTTCACCTGCATACGACCGAGCACCACCGCCTTATGGCCTGACTTTACCTGCATATAGTCCCACTTCTCCTTTGTACACCCCAACATCACCCTCCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTTCCCCACATACATCCCCACTTCGCTTTCTTACAGCCCCACATCGCCATCATACAGCCACACATCGCCCTCCTACAGCCCCACATCCCCATCATACAGCCCCACATCACCCTCCTACAGCCCTAGCTCCCCATACGATAGCCTGACATCTCCAGGTTACAACCCAACCTCTCCCAGTTATAGCCTGACATCTCTGTACAGCAGCGCAACTTCACTAGCATATTCCCCAACATCTCCAACTTACTCTCCATCGAGCCCCACTTACAGTCCCAGCAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1772

Amino Acids

198.5

Weight (kDa)

5.94

Isoelectric Point (pI)

57.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	16 - 350	9.6e-101	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	352 - 520	3.6e-69	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	523 - 687	1.2e-53	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	714 - 818	3.9e-39	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	825 - 1414	2.1e-105	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	891 - 1075	1.9e-65	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1160 - 1293	4e-50	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1329, 1781
AarI	CACCTGC	1 cut(s)	4927
AasI	GACNNNNNNGTC	2 cut(s)	295, 2439
Acc36I	ACCTGC	4 cut(s)	2475, 4927, 4969, 5304
AccB1I	GGYRCC	3 cut(s)	1558, 4155, 4357
AccB7I	CCANNNNNTGG	1 cut(s)	691
AccI	GTMKAC	1 cut(s)	4653
AccIII	TCCGGA	2 cut(s)	2776, 3523
AciI	CCGC	5 cut(s)	150, 1217, 3956, 4516, 4940
AcoI	YGGCCR	7 cut(s)	115, 241, 1030, 1267, 2918, 4161, 4203
AcsI	RAATTY	8 cut(s)	850, 1985, 2376, 2458, 2626, 2729, 3560, 4581
AcuI	CTGAAG	9 cut(s)	1797, 2868, 2957, 3068, 3444, 3840, 3857, 4002, 4278
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	6 cut(s)	566, 1128, 1250, 3161, 4988, 5237
AfiI	CCNNNNNNNGG	8 cut(s)	146, 170, 691, 1229, 1601, 2151, 4159, 4946
AflII	CTTAAG	1 cut(s)	1016
AflIII	ACRYGT	2 cut(s)	487, 1945
AjiI	CACGTC	3 cut(s)	4686, 4730, 4787
AjuI	GAANNNNNNNTTGG	2 cut(s)	251, 283
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	7 cut(s)	50, 255, 520, 1551, 1633, 3539, 4936
AlwNI	CAGNNNCTG	1 cut(s)	1166
Ama87I	CYCGRG	1 cut(s)	229
Aor13HI	TCCGGA	2 cut(s)	2776, 3523
ApoI	RAATTY	8 cut(s)	850, 1985, 2376, 2458, 2626, 2729, 3560, 4581
ArsI	GACNNNNNNTTYG	2 cut(s)	1266, 1298
AseI	ATTAAT	1 cut(s)	3699
Asp700I	GAANNNNTTC	3 cut(s)	600, 645, 2989
AspLEI	GCGC	2 cut(s)	309, 5246
AspS9I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1967, 2432, 3526, 4241
AsuC2I	CCSGG	4 cut(s)	75, 230, 231, 773
AsuII	TTCGAA	1 cut(s)	2855
AsuNHI	GCTAGC	1 cut(s)	3137
AvaI	CYCGRG	1 cut(s)	229
AvaII	GGWCC	5 cut(s)	173, 1967, 2432, 3526, 4241
BaeGI	GKGCMC	5 cut(s)	230, 1563, 1918, 2254, 4384
BaeI	ACNNNNGTAYC	4 cut(s)	1851, 1884, 2523, 2556
BalI	TGGCCA	3 cut(s)	1269, 4163, 4205
BamHI	GGATCC	2 cut(s)	3882, 4136
BanI	GGYRCC	3 cut(s)	1558, 4155, 4357
BanII	GRGCYC	6 cut(s)	50, 255, 473, 1551, 2230, 5297
BarI	GAAGNNNNNNTAC	4 cut(s)	4389, 4413, 4421, 4445
BauI	CACGAG	2 cut(s)	2229, 3171
BbsI	GAAGAC	1 cut(s)	293
Bbv12I	GWGCWC	7 cut(s)	50, 255, 520, 1551, 1633, 3539, 4936
BbvCI	CCTCAGC	1 cut(s)	68
BceAI	ACGGC	2 cut(s)	102, 2905
BciVI	GTATCC	2 cut(s)	1645, 3970
BclI	TGATCA	4 cut(s)	1114, 1306, 3811, 3934
BcnI	CCSGG	4 cut(s)	75, 230, 231, 773
BfaI	CTAG	8 cut(s)	1383, 1530, 3138, 3344, 4346, 4506, 5163, 5255
BfrI	CTTAAG	1 cut(s)	1016
BfuAI	ACCTGC	4 cut(s)	2475, 4927, 4969, 5304
BfuI	GTATCC	2 cut(s)	1645, 3970
BglI	GCCNNNNNGGC	1 cut(s)	240
BlpI	GCTNAGC	1 cut(s)	519
Bme18I	GGWCC	5 cut(s)	173, 1967, 2432, 3526, 4241
BmeT110I	CYCGRG	1 cut(s)	229
BmgBI	CACGTC	3 cut(s)	4686, 4730, 4787
BmgT120I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1967, 2432, 3526, 4241
BmiI	GGNNCC	7 cut(s)	14, 1560, 3528, 3884, 4138, 4157, 4359
BmrI	ACTGGG	4 cut(s)	1996, 2565, 4518, 5208
BmtI	GCTAGC	1 cut(s)	3141
BmuI	ACTGGG	4 cut(s)	1996, 2565, 4518, 5208
BoxI	GACNNNNGTC	1 cut(s)	4239
BpiI	GAAGAC	1 cut(s)	293
BplI	GAGNNNNNCTC	2 cut(s)	148, 180
BpmI	CTGGAG	8 cut(s)	50, 122, 1565, 1787, 2180, 4392, 4590, 5175
Bpu10I	CCTNAGC	2 cut(s)	68, 1106
Bpu1102I	GCTNAGC	1 cut(s)	519
Bpu14I	TTCGAA	1 cut(s)	2855
BpuEI	CTTGAG	5 cut(s)	175, 238, 2267, 3483, 4508
BpuMI	CCSGG	4 cut(s)	75, 230, 231, 773
BsaBI	GATNNNNATC	2 cut(s)	1305, 4205
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	1 cut(s)	760
BsaWI	WCCGGW	6 cut(s)	9, 479, 2397, 2434, 2776, 3523
Bsc4I	CCNNNNNNNGG	8 cut(s)	146, 170, 691, 1229, 1601, 2151, 4159, 4946
Bse118I	RCCGGY	4 cut(s)	139, 1590, 3317, 4152
Bse3DI	GCAATG	3 cut(s)	1137, 3684, 4097
Bse8I	GATNNNNATC	2 cut(s)	1305, 4205
BseAI	TCCGGA	2 cut(s)	2776, 3523
BseJI	GATNNNNATC	2 cut(s)	1305, 4205
BseLI	CCNNNNNNNGG	8 cut(s)	146, 170, 691, 1229, 1601, 2151, 4159, 4946
BseMI	GCAATG	3 cut(s)	1137, 3684, 4097
BseRI	GAGGAG	1 cut(s)	2584
BseSI	GKGCMC	5 cut(s)	230, 1563, 1918, 2254, 4384
BseYI	CCCAGC	2 cut(s)	2832, 5309
BsgI	GTGCAG	2 cut(s)	3246, 4445
Bsh1285I	CGRYCG	1 cut(s)	4930
BshNI	GGYRCC	3 cut(s)	1558, 4155, 4357
BsiEI	CGRYCG	1 cut(s)	4930
BsiHKAI	GWGCWC	7 cut(s)	50, 255, 520, 1551, 1633, 3539, 4936
BsiHKCI	CYCGRG	1 cut(s)	229
BslFI	GGGAC	9 cut(s)	708, 1936, 2364, 2629, 4506, 4644, 4695, 4956, 5292
BslI	CCNNNNNNNGG	8 cut(s)	146, 170, 691, 1229, 1601, 2151, 4159, 4946
BsmBI	CGTCTC	2 cut(s)	3779, 4506
BsmFI	GGGAC	9 cut(s)	708, 1936, 2364, 2629, 4506, 4644, 4695, 4956, 5292
BsmI	GAATGC	1 cut(s)	2959
Bso31I	GGTCTC	1 cut(s)	760
BsoBI	CYCGRG	1 cut(s)	229
Bsp119I	TTCGAA	1 cut(s)	2855
Bsp13I	TCCGGA	2 cut(s)	2776, 3523
Bsp1407I	TGTACA	2 cut(s)	4986, 5235
Bsp1720I	GCTNAGC	1 cut(s)	519
Bsp19I	CCATGG	2 cut(s)	1130, 2289
BspACI	CCGC	5 cut(s)	150, 1217, 3956, 4516, 4940
BspEI	TCCGGA	2 cut(s)	2776, 3523
BspHI	TCATGA	2 cut(s)	1551, 3777
BspLI	GGNNCC	7 cut(s)	14, 1560, 3528, 3884, 4138, 4157, 4359
BspMAI	CTGCAG	3 cut(s)	468, 640, 2539
BspMI	ACCTGC	4 cut(s)	2475, 4927, 4969, 5304
BspOI	GCTAGC	1 cut(s)	3141
BspQI	GCTCTTC	1 cut(s)	3879
BspT104I	TTCGAA	1 cut(s)	2855
BspT107I	GGYRCC	3 cut(s)	1558, 4155, 4357
BspTI	CTTAAG	1 cut(s)	1016
BspTNI	GGTCTC	1 cut(s)	760
BsrDI	GCAATG	3 cut(s)	1137, 3684, 4097
BsrFI	RCCGGY	4 cut(s)	139, 1590, 3317, 4152
BsrGI	TGTACA	2 cut(s)	4986, 5235
BssAI	RCCGGY	4 cut(s)	139, 1590, 3317, 4152
BssNAI	GTATAC	1 cut(s)	4654
BssNI	GRCGYC	1 cut(s)	41
BssSI	CACGAG	2 cut(s)	2229, 3171
BssT1I	CCWWGG	6 cut(s)	1130, 2106, 2289, 3353, 3438, 3620
Bst1107I	GTATAC	1 cut(s)	4654
Bst2BI	CACGAG	2 cut(s)	2229, 3171
Bst6I	CTCTTC	9 cut(s)	1052, 1446, 1953, 2252, 2933, 3543, 3588, 3879, 4392
BstACI	GRCGYC	1 cut(s)	41
BstAFI	CTTAAG	1 cut(s)	1016
BstAUI	TGTACA	2 cut(s)	4986, 5235
BstBI	TTCGAA	1 cut(s)	2855
BstDSI	CCRYGG	2 cut(s)	1130, 2289
BstHHI	GCGC	2 cut(s)	309, 5246
BstMCI	CGRYCG	1 cut(s)	4930
BstMWI	GCNNNNNNNGC	9 cut(s)	69, 240, 259, 315, 324, 2072, 2843, 3686, 4094
BstNSI	RCATGY	5 cut(s)	423, 491, 1949, 3859, 4282
BstPAI	GACNNNNGTC	1 cut(s)	4239
BstSLI	GKGCMC	5 cut(s)	230, 1563, 1918, 2254, 4384
BstV2I	GAAGAC	1 cut(s)	293
BstX2I	RGATCY	5 cut(s)	1096, 2806, 3553, 3882, 4136
BstXI	CCANNNNNNTGG	1 cut(s)	2158
BstYI	RGATCY	5 cut(s)	1096, 2806, 3553, 3882, 4136
BstZ17I	GTATAC	1 cut(s)	4654
BsuI	GTATCC	2 cut(s)	1645, 3970
BtgI	CCRYGG	2 cut(s)	1130, 2289
BtgZI	GCGATG	3 cut(s)	4267, 5067, 5088
BtrI	CACGTC	3 cut(s)	4686, 4730, 4787
BveI	ACCTGC	4 cut(s)	2475, 4927, 4969, 5304
CaiI	CAGNNNCTG	1 cut(s)	1166
CciI	TCATGA	2 cut(s)	1551, 3777
CfoI	GCGC	2 cut(s)	309, 5246
Cfr10I	RCCGGY	4 cut(s)	139, 1590, 3317, 4152
Cfr13I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1967, 2432, 3526, 4241
Cfr9I	CCCGGG	1 cut(s)	229
CseI	GACGC	4 cut(s)	49, 1328, 2684, 2852
CsiI	ACCWGGT	5 cut(s)	2211, 4432, 4690, 4747, 4804
Csp6I	GTAC	6 cut(s)	565, 1127, 1249, 3160, 4987, 5236
CspCI	CAANNNNNGTGG	2 cut(s)	919, 954
CviQI	GTAC	6 cut(s)	565, 1127, 1249, 3160, 4987, 5236
DraI	TTTAAA	2 cut(s)	364, 2712
DrdI	GACNNNNNNGTC	2 cut(s)	295, 2439
DseDI	GACNNNNNNGTC	2 cut(s)	295, 2439
EaeI	YGGCCR	7 cut(s)	115, 241, 1030, 1267, 2918, 4161, 4203
Eam1104I	CTCTTC	9 cut(s)	1052, 1446, 1953, 2252, 2933, 3543, 3588, 3879, 4392
EarI	CTCTTC	9 cut(s)	1052, 1446, 1953, 2252, 2933, 3543, 3588, 3879, 4392
Ecl136II	GAGCTC	3 cut(s)	48, 253, 1549
Eco130I	CCWWGG	6 cut(s)	1130, 2106, 2289, 3353, 3438, 3620
Eco24I	GRGCYC	6 cut(s)	50, 255, 473, 1551, 2230, 5297
Eco31I	GGTCTC	1 cut(s)	760
Eco47I	GGWCC	5 cut(s)	173, 1967, 2432, 3526, 4241
Eco53kI	GAGCTC	3 cut(s)	48, 253, 1549
Eco57I	CTGAAG	9 cut(s)	1797, 2868, 2957, 3068, 3444, 3840, 3857, 4002, 4278
Eco88I	CYCGRG	1 cut(s)	229
EcoICRI	GAGCTC	3 cut(s)	48, 253, 1549
EcoRI	GAATTC	3 cut(s)	2376, 2458, 4581
EcoT14I	CCWWGG	6 cut(s)	1130, 2106, 2289, 3353, 3438, 3620
EcoT22I	ATGCAT	2 cut(s)	421, 1571
EcoT38I	GRGCYC	6 cut(s)	50, 255, 473, 1551, 2230, 5297
ErhI	CCWWGG	6 cut(s)	1130, 2106, 2289, 3353, 3438, 3620
Esp3I	CGTCTC	2 cut(s)	3779, 4506
FalI	AAGNNNNNCTT	2 cut(s)	3540, 3572
FaqI	GGGAC	9 cut(s)	708, 1936, 2364, 2629, 4506, 4644, 4695, 4956, 5292
FauNDI	CATATG	1 cut(s)	1952
FbaI	TGATCA	4 cut(s)	1114, 1306, 3811, 3934
FblI	GTMKAC	1 cut(s)	4653
FriOI	GRGCYC	6 cut(s)	50, 255, 473, 1551, 2230, 5297
FspBI	CTAG	8 cut(s)	1383, 1530, 3138, 3344, 4346, 4506, 5163, 5255
GlaI	GCGC	2 cut(s)	308, 5245
GsaI	CCCAGC	2 cut(s)	2836, 5313
GsuI	CTGGAG	8 cut(s)	50, 122, 1565, 1787, 2180, 4392, 4590, 5175
HgaI	GACGC	4 cut(s)	49, 1328, 2684, 2852
HhaI	GCGC	2 cut(s)	309, 5246
Hin1I	GRCGYC	1 cut(s)	41
Hin6I	GCGC	2 cut(s)	307, 5244
HinP1I	GCGC	2 cut(s)	307, 5244
HincII	GTYRAC	3 cut(s)	485, 2932, 3925
HindII	GTYRAC	3 cut(s)	485, 2932, 3925
HindIII	AAGCTT	2 cut(s)	369, 3164
HpaI	GTTAAC	2 cut(s)	2932, 3925
Hpy166II	GTNNAC	9 cut(s)	485, 903, 2209, 2391, 2932, 3925, 4276, 4654, 4989
Hpy8I	GTNNAC	9 cut(s)	485, 903, 2209, 2391, 2932, 3925, 4276, 4654, 4989
Hpy99I	CGWCG	2 cut(s)	43, 4095
HpyCH4IV	ACGT	6 cut(s)	1503, 1671, 4572, 4685, 4729, 4786
HpyF10VI	GCNNNNNNNGC	9 cut(s)	69, 240, 259, 315, 324, 2072, 2843, 3686, 4094
HpySE526I	ACGT	6 cut(s)	1503, 1671, 4572, 4685, 4729, 4786
Hsp92I	GRCGYC	1 cut(s)	41
HspAI	GCGC	2 cut(s)	307, 5244
Kpn2I	TCCGGA	2 cut(s)	2776, 3523
Ksp22I	TGATCA	4 cut(s)	1114, 1306, 3811, 3934
KspAI	GTTAAC	2 cut(s)	2932, 3925
LguI	GCTCTTC	1 cut(s)	3879
MabI	ACCWGGT	5 cut(s)	2211, 4432, 4690, 4747, 4804
MaeI	CTAG	8 cut(s)	1383, 1530, 3138, 3344, 4346, 4506, 5163, 5255
MaeII	ACGT	6 cut(s)	1503, 1671, 4572, 4685, 4729, 4786
MfeI	CAATTG	3 cut(s)	322, 2061, 4362
MflI	RGATCY	5 cut(s)	1096, 2806, 3553, 3882, 4136
MlsI	TGGCCA	3 cut(s)	1269, 4163, 4205
MluNI	TGGCCA	3 cut(s)	1269, 4163, 4205
MlyI	GAGTC	6 cut(s)	283, 1071, 1165, 1269, 3289, 4528
MmeI	TCCRAC	5 cut(s)	4527, 4784, 4841, 4913, 5300
Mox20I	TGGCCA	3 cut(s)	1269, 4163, 4205
Mph1103I	ATGCAT	2 cut(s)	421, 1571
MroI	TCCGGA	2 cut(s)	2776, 3523
MroXI	GAANNNNTTC	3 cut(s)	600, 645, 2989
MscI	TGGCCA	3 cut(s)	1269, 4163, 4205
MslI	CAYNNNNRTG	8 cut(s)	1142, 1556, 1688, 3184, 3290, 3638, 4017, 4053
Msp20I	TGGCCA	3 cut(s)	1269, 4163, 4205
MspA1I	CMGCKG	3 cut(s)	761, 881, 2158
MspCI	CTTAAG	1 cut(s)	1016
MssI	GTTTAAAC	1 cut(s)	364
MunI	CAATTG	3 cut(s)	322, 2061, 4362
Mva1269I	GAATGC	1 cut(s)	2959
MwoI	GCNNNNNNNGC	9 cut(s)	69, 240, 259, 315, 324, 2072, 2843, 3686, 4094
NciI	CCSGG	4 cut(s)	75, 230, 231, 773
NcoI	CCATGG	2 cut(s)	1130, 2289
NdeI	CATATG	1 cut(s)	1952
NheI	GCTAGC	1 cut(s)	3137
NlaIV	GGNNCC	7 cut(s)	14, 1560, 3528, 3884, 4138, 4157, 4359
NmeAIII	GCCGAG	2 cut(s)	69, 145
NmuCI	GTSAC	6 cut(s)	158, 1168, 1459, 2293, 2899, 4686
NsiI	ATGCAT	2 cut(s)	421, 1571
NspI	RCATGY	5 cut(s)	423, 491, 1949, 3859, 4282
NspV	TTCGAA	1 cut(s)	2855
PaeI	GCATGC	1 cut(s)	423
PagI	TCATGA	2 cut(s)	1551, 3777
PaqCI	CACCTGC	1 cut(s)	4927
PciI	ACATGT	2 cut(s)	487, 1945
PciSI	GCTCTTC	1 cut(s)	3879
PctI	GAATGC	1 cut(s)	2959
PdmI	GAANNNNTTC	3 cut(s)	600, 645, 2989
PflFI	GACNNNGTC	1 cut(s)	1361
PflMI	CCANNNNNTGG	1 cut(s)	691
PleI	GAGTC	6 cut(s)	283, 1070, 1165, 1269, 3289, 4527
PmeI	GTTTAAAC	1 cut(s)	364
PpsI	GAGTC	6 cut(s)	283, 1070, 1165, 1269, 3289, 4527
PscI	ACATGT	2 cut(s)	487, 1945
PshAI	GACNNNNGTC	1 cut(s)	4239
PshBI	ATTAAT	1 cut(s)	3699
PsiI	TTATAA	2 cut(s)	1329, 1781
Psp124BI	GAGCTC	3 cut(s)	50, 255, 1551
PspFI	CCCAGC	2 cut(s)	2832, 5309
PspN4I	GGNNCC	7 cut(s)	14, 1560, 3528, 3884, 4138, 4157, 4359
PspPI	GGNCC	8 cut(s)	173, 232, 990, 1214, 1967, 2432, 3526, 4241
PstI	CTGCAG	3 cut(s)	468, 640, 2539
PstNI	CAGNNNCTG	1 cut(s)	1166
PsuI	RGATCY	5 cut(s)	1096, 2806, 3553, 3882, 4136
PsyI	GACNNNGTC	1 cut(s)	1361
PvuII	CAGCTG	3 cut(s)	761, 881, 2158
RsaI	GTAC	6 cut(s)	566, 1128, 1250, 3161, 4988, 5237
RsaNI	GTAC	6 cut(s)	565, 1127, 1249, 3160, 4987, 5236
RseI	CAYNNNNRTG	8 cut(s)	1142, 1556, 1688, 3184, 3290, 3638, 4017, 4053
SacI	GAGCTC	3 cut(s)	50, 255, 1551
SapI	GCTCTTC	1 cut(s)	3879
Sau96I	GGNCC	8 cut(s)	173, 232, 990, 1214, 1967, 2432, 3526, 4241
SchI	GAGTC	6 cut(s)	283, 1071, 1165, 1269, 3289, 4528
SexAI	ACCWGGT	5 cut(s)	2211, 4432, 4690, 4747, 4804
SfiI	GGCCNNNNNGGCC	1 cut(s)	240
SfuI	TTCGAA	1 cut(s)	2855
SinI	GGWCC	5 cut(s)	173, 1967, 2432, 3526, 4241
SmaI	CCCGGG	1 cut(s)	231
SmiMI	CAYNNNNRTG	8 cut(s)	1142, 1556, 1688, 3184, 3290, 3638, 4017, 4053
SmlI	CTYRAG	6 cut(s)	154, 217, 1016, 2282, 3498, 4487
SmoI	CTYRAG	6 cut(s)	154, 217, 1016, 2282, 3498, 4487
SphI	GCATGC	1 cut(s)	423
SrfI	GCCCGGGC	1 cut(s)	231
SsiI	CCGC	5 cut(s)	150, 1217, 3956, 4516, 4940
SspI	AATATT	2 cut(s)	1734, 3115
SspMI	CTAG	8 cut(s)	1383, 1530, 3138, 3344, 4346, 4506, 5163, 5255
SstI	GAGCTC	3 cut(s)	50, 255, 1551
StyI	CCWWGG	6 cut(s)	1130, 2106, 2289, 3353, 3438, 3620
TaiI	ACGT	6 cut(s)	1506, 1674, 4575, 4688, 4732, 4789
TaqII	GACCGA	1 cut(s)	4944
TatI	WGTACW	5 cut(s)	564, 1248, 3159, 4986, 5235
TauI	GCSGC	2 cut(s)	153, 1219
TseFI	GTSAC	6 cut(s)	158, 1168, 1459, 2293, 2899, 4686
Tsp45I	GTSAC	6 cut(s)	158, 1168, 1459, 2293, 2899, 4686
TspGWI	ACGGA	4 cut(s)	40, 1093, 2370, 3761
TspMI	CCCGGG	1 cut(s)	229
Tth111I	GACNNNGTC	1 cut(s)	1361
Van91I	CCANNNNNTGG	1 cut(s)	691
Vha464I	CTTAAG	1 cut(s)	1016
VpaK11BI	GGWCC	5 cut(s)	173, 1967, 2432, 3526, 4241
VspI	ATTAAT	1 cut(s)	3699
XapI	RAATTY	8 cut(s)	850, 1985, 2376, 2458, 2626, 2729, 3560, 4581
XceI	RCATGY	5 cut(s)	423, 491, 1949, 3859, 4282
XcmI	CCANNNNNNNNNTGG	2 cut(s)	535, 1749
XmaI	CCCGGG	1 cut(s)	229
XmiI	GTMKAC	1 cut(s)	4653
XmnI	GAANNNNTTC	3 cut(s)	600, 645, 2989
XspI	CTAG	8 cut(s)	1383, 1530, 3138, 3344, 4346, 4506, 5163, 5255
Zsp2I	ATGCAT	2 cut(s)	421, 1571

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.