Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Reverse (-)

9371578 .. 9378790

Gene Structure 7213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G009330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5436 bp

ATGGATATTCGCTTCCCTTTCTCGCCGTGCGAGGTCGCCAAGGTCCGCCGCGTCCAGTTCGGCATACTGAGCCCCGACGAAATCAGGCAAATGTCTGTGGTGCAGATTGAGCACAGTGAAACAATGCTGGGGGGAAAGCCGAAGACGGCGGGGTTGAGTGACCCTCGGCTGGGGACAATTGACCGGAAGATGAAGTGTGAGACTTGCACGGCCAACATGGCTGAGTGCCCGGGTCACTTTGGGCACCTGGAGCTGGCCAAGCCCATGTTTCATATTGGGTTCATGAAGACTGTGTTGTCTATAATGCGCTGCGTCTGCTTCAACTGCTCCAAAATCCTAGTTGATGAGGAGGAGCACAAGTTTAAGACAGCTTTGAGAATCAAGAACCCAAAAAATAGGCTAAAGAAAATTTTGGACGCGTGCAAAAACAAAACCAAGTGTGAAGGCGGTGATGAGATTGATGTGCAAGAATCGGACGAACCTTTAAAGAAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCGAAGCTCTCTATAGATGGTATGAAGATGGTTGCAGAGTACAAAGCCCAAAGAAAGAAGAGTGATGACCAGGAACAGCTTCCTGAACCTGTGGAAAGGAAGCAGACCCTTACCGCTGAAAGGGTTCTTAGTGTGTTGAAGAGGATAAGTGATGAAGATTGCCAATTATTGGGATTGAACCCCAAGTATGCACGTCCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACAACCTGTGAGACCCTCTGTGATGATGGACACTCCTCGAGGAGTGAGGCAAAAACGGATCACCGCTCATATCATCTCAGAGTTTGCACAACTCTTGCAATTCCATATAGCTACATATTTTGATAATGAGTTGCCTGGACTACCAAGGGCCACACAGAGATCAGGGAGGCCTATTAAATCAATTTGTAGTAGGCTTAAGGCGAAGGAAGGTCGGATTAGAGGAAACTTAATGGGGAAGAGGGTTGATTTCTCAGCACGTACGGTTATTACACCAGATCCGAACATTAACATTGATCAATTAGGAGTACCATGGAGTATTGCGCTGAATCTCACATACCCTGAAACTGTGACTCCTTATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTACGGGCCCCATCCTCCACCAGGTAAAACTGGTGCAAAGTATATAATAAGAGATGATGGGCAAAGGCTTGATCTACGGTACTTGAAGAAAAGTAGTGATCATCATTTGGAGCTTGGTTATAAGGTGGAGCGTCATTTGAATGATGGAGACTTTGTCCTCTTTAATCGTCAACCCAGTCTCCATAAAATGTCTATTATGGGGCACAGAATCAAAATCATGCCTTACTCAACTTTTCGATTAAATCTATCTGTCACTTCGCCTTATAATGCTGATTTTGATGGGGATGAAATGAATATGCACGTTCCCCAGTCATTTGAAACTAGGGCAGAAGTGTTGGAGCTCATGATGGTGCCCAAATGCATTGTGTCCCCTCAATCAAATCGTCCTGTGATGGGAATAGTCCAAGATACCCTGTTGGGTTGTCGTAAGATCACCAAGAGGGATACCTTCATAGAGAAGGATGTTTTCATGAACATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTACCTGCTCCTGCAATATTGAAACCACGGCCTCTTTGGACTGGGAAACAGGTGTTTAATCTTATAATACCAAAGCAAATAAATCTCCAGAGAACTTCAGCGTGGCACTCTGAATCAGAAACTGGTAACATTACCCCTGGTGATACTCACGTTAGAATAGAAAAAGGGGAGTTACTTTCCGGAACTCTGTGCAAAAAGGCACTTGGGACATCTACTGGAAGTCTTATACACGTCATATGGGAAGAGGTTGGTCCAGATGCTGCCCGCAAATTCCTTGGTCATACCCAGTGGCTTGTGAATTACTGGCTATTGCAGAATGCATTTAGCATTGGAATTGGAGATACAATTGCTGATGCAGCAACGATGGAAAAGATCAATGAAACTATTTCCAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAAGAGCCTGGAGCCTGAACCGGGGCGAACTATGATGGATTCCTTTGAAAACAAAGTGAATCAGGTCTTAAATAGGGCTCGTGATGATGCTGGAAGTAGTGCTCAGAAGAGTTTGTCTGAGAGTAACAATCTTAAGGCTATGGTTACAGCTGGCTCTAAGGGAAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAACGAATCCCATATGGGTTCATAGACCGTACTTTGCCCCACTTCACTAAGGACGACTATGGACCAGAAAGTCGTGGCTTTGTAGAGAATTCATACCTTCGAGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGAGGTAGGGAAGGTCTTATAGATACTGCAGTTAAGACATCCGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTCAAATATGATGGGACTGTGAGAAATTCGTTGGGGGACGTCATACAGTTTCTCTATGGAGAAGATGGAATGGATGCTGTATGGATAGAATCACAAAAGCTGGATTCTCTTAAAATGAAGAAGAAAGAGTTTGACAAGACTTTCGCTTATGTGTTTGATGATGAAAATTGGAATCCGGACTATATGTTGCCTGAACATGTTGAGGATCTAAAGACTATTAGAGAGTTCCGCAATGTGTTTGATGCAGAAGTTTCAAAACTTGAAACTGATAGGCTTCAACTTGGCACAGAGATTGCTGTCACTGGTGATAATTCTTGGCCATTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTTAAAATCGACTTCAGGAGGACTTCTGATATGCATCCAATGGAAATTGTTGAGGCTATTGATAAACTCCAGGAGAGGCTGAGGGTTGTTCCTGGTGATGATTTGTTGAGTGTTGAAGCCCAAAAGAATGCAACTCTCTTTTTCAACATTTTGCTTCGTAGCACTTTCGCTAGTAAAAGGGTGTTGGAGGAGTACAGGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGTCACGTTTCCTGCAATCACTGGTAGCGCCTGGTGAAATGATTGGTTGTGTTGCTGCACAATCTATTGGTGAGCCTGCCACTCAGATGACCCTCAATACCTTCCATTATGCTGGTGTGAGTGCAAAAAATGTTACACTAGGTGTTCCCAGGTTGAGGGAAATTATTAATGTTGCCAAAAGGATCAAAACACCATCTCTGTCTGTCTACTTAAAGCCTGAAGCTAATAAAACCAAGGAGAGGGCCAAGAATGTGCAGTGTGCCTTGGAATACACTACCCTACGAAGTGTTACTCAGGCTACAGAAGTATGGTATGATCCAGATCCAATGAGCACATTAATAGAAGAGGATCTTGATTTTGTGAGATCTTACTACGAAATGCCAGATGAAGAGGTCAACCCCGACAAAATTTCTCCGTGGCTGCTTCGCATTGAGTTGAATCGCGAAATGATGGTGGATAAGAAATTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGATGGTGATTTAACTTGTATTTTTAATGATGACAACGCCGAAAAGTTGATCCTTCGAATTCGTATCATGAATGAGGAAACTCCTAAAGGGGAATCACAAGATGAATCTGCTGAAGATGATGTATTCCTGAAGAAGATTGAAAGTAATATGCTTACAGAAATGGCTCTTCGAGGTATCCCAGATATCAACAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGATTCAAGGCAGAGCAAGAGTGGATGTTGGATACAGAGGGTGTTAATCTTTTAGCTGTCCTGTGCCATGATGATGTTGATGCCAGGAGAACGACAAGCAATCATTTGATTGAAATTATTGAAGTTCTTGGGATTGAGGCAGTTCGCCGGTCTTTGCTGGATGAGTTGCGAGTTGTCATATCGTTTGATGGATCTTATGTGAACTACCGCCATCTGGCTATCCTATGTGATACCATGACGTATCGTGGCCACTTGATGGCCATTACTCGTCATGGTATAAATAGAAATGATACCGGCCCAATGATGCGTTGTTCGTTCGAAGAAACTGTGGATATTCTGCTGGATGCTTCTGTTTTTGCAGAAACGGATCATCTGAGGGGTGTAACAGAAAACATTATGCTAGGCCAGTTGGCACCAATTGGGACTGGAGACTGTGCCCTGTATCTCAATGATGAGATGCTAAAGAATGCTATTGAACTCCAGCTGCCTAGTTATATGGATGGCCTTGATTTTGGCATGACCCCATCGCGTTCTCCAGTGTCAGGAACTCCATATCGTGATGGAATGATGTCACCTAATTATTTGCTGAGCCCAAATATGCGAATGTCTCCCATCTCAGATGCTCAGTTTTCTCCCTATGTCGGTGGAATGGCATTCTCTCCTACTTCATCTCCAGGCTACAGCCCATCATCTCCTGGCTACAGTCCATCCTCCCCTGGTTACAGCCCCACGTCCCCTGGTTACAGCCCCACGTCCCCAGGTTACAGCCCCACCTCCCCTGGTTATAGTCCCACTTCTCCAACCTATAGTCCAAGCTCTCCAGGTTATAGTCCAACCAGTCCGGCATACTCTCCTACAAGTCCGTCTTACTCTCCTACATCTCCAAGCTACAGCCCTACCTCTCCAAGCTACAGTCCAACCTCTCCAAGCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTCCCCCGCTTACAGCCCGACTTCCCCCGCATACAGCCCCACTTCCCCCGCTTACAGCCCCACTTCCCCATCATACAGCCCAACATCTCCCTCATACAGCCCCACTTCACCCTCCTACAGCCCCACTTCGCCCTCCTACAGCCCCACATCCCCCTCCTACAGCCCAACATCCCCCTCCTACAGCCCAACTTCACCTGCCTACAGCCCAACTTCTCCTGGTTACAGCCCTACTTCCCCAAGTTATAGTCCAACCTCTCCTAGTTATAGCCCTACCTCTCCAAGTTACAATCCTCCTTCTGCAAAATATAGCCCATCGGTAGCTTACTCTCCGAGCAGTCCAAGATTGTCACCAGCTAGTCCATATAGTCCTACCTCTCCAAATTACAGCCCCACATCACCGTCATATTCTCCCACATCACCATCTTATTCTCCCTCTAGCCCCTCATACACCCCCGGCAGGACACTGATGTATGCATCTTTAATTTTCCAAAAAAATGTGGCGAGTGCTCATTCAAATTCCTCTTCTTACTTCTCCTTGCAGTCCAAGTGCTGGATACTCACCCAGTCAGCCAGGGTACTCCCCATCATCTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1811

Amino Acids

202.27

Weight (kDa)

6.3

Isoelectric Point (pI)

62.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	14 - 262	4.6e-76	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_1	PF04997	268 - 327	2e-18	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	329 - 497	1.1e-71	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	500 - 664	1.2e-52	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	691 - 795	9.5e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	802 - 1392	2.9e-104	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	868 - 1052	2e-67	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1137 - 1271	1.2e-53	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1110, 1260, 1404, 1712
AarI	CACCTGC	1 cut(s)	5044
AasI	GACNNNNNNGTC	2 cut(s)	295, 2370
AatII	GACGTC	1 cut(s)	2574
AbsI	CCTCGAGG	1 cut(s)	789
Acc36I	ACCTGC	2 cut(s)	1660, 5044
AccB1I	GGYRCC	3 cut(s)	243, 1489, 4291
AccB7I	CCANNNNNTGG	3 cut(s)	688, 4135, 5391
AccBSI	CCGCTC	1 cut(s)	818
AccI	GTMKAC	1 cut(s)	3342
AccII	CGCG	4 cut(s)	51, 419, 3579, 4408
AccIII	TCCGGA	2 cut(s)	1826, 2707
AcoI	YGGCCR	5 cut(s)	210, 255, 2849, 4126, 4137
AcsI	RAATTY	8 cut(s)	408, 1916, 2389, 2557, 3543, 3638, 3699, 5356
AcuI	CTGAAG	6 cut(s)	1728, 2888, 2896, 3375, 3774, 3791
AcyI	GRCGYC	1 cut(s)	2571
AdeI	CACNNNGTG	1 cut(s)	3278
AfaI	GTAC	9 cut(s)	560, 1012, 1059, 1142, 1220, 1650, 2332, 3092, 5418
AflII	CTTAAG	2 cut(s)	947, 2213
AflIII	ACRYGT	3 cut(s)	417, 1876, 2728
AjiI	CACGTC	4 cut(s)	713, 1879, 4611, 4632
Alw21I	GWGCWC	7 cut(s)	114, 357, 514, 1482, 2185, 3470, 5350
Alw26I	GTCTC	8 cut(s)	194, 757, 1281, 1322, 2477, 2497, 4302, 4491
AlwNI	CAGNNNCTG	2 cut(s)	1769, 1907
Ama87I	CYCGRG	2 cut(s)	229, 789
Aor13HI	TCCGGA	2 cut(s)	1826, 2707
ApaI	GGGCCC	1 cut(s)	1149
ApeKI	GCWGC	7 cut(s)	309, 517, 1907, 2003, 3191, 3556, 4363
ApoI	RAATTY	8 cut(s)	408, 1916, 2389, 2557, 3543, 3638, 3699, 5356
ArsI	GACNNNNNNTTYG	2 cut(s)	2657, 2689
AseI	ATTAAT	2 cut(s)	3303, 3473
Asp700I	GAANNNNTTC	2 cut(s)	597, 642
AspLEI	GCGC	3 cut(s)	309, 1075, 3166
AspS9I	GGNCC	8 cut(s)	43, 900, 1145, 1146, 1898, 2363, 3378, 4175
AsuC2I	CCSGG	4 cut(s)	230, 231, 2103, 5295
AsuII	TTCGAA	2 cut(s)	3697, 4197
AvaI	CYCGRG	2 cut(s)	229, 789
AvaII	GGWCC	3 cut(s)	43, 1898, 2363
BaeGI	GKGCMC	6 cut(s)	230, 246, 1149, 1344, 1494, 4318
BaeI	ACNNNNGTAYC	4 cut(s)	1782, 1815, 2454, 2487
BalI	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
BanI	GGYRCC	3 cut(s)	243, 1489, 4291
BanII	GRGCYC	5 cut(s)	74, 1149, 1482, 2161, 4472
BauI	CACGAG	2 cut(s)	2160, 3102
BbsI	GAAGAC	2 cut(s)	149, 293
Bbv12I	GWGCWC	7 cut(s)	114, 357, 514, 1482, 2185, 3470, 5350
BbvCI	CCTCAGC	1 cut(s)	2978
BbvI	GCAGC	7 cut(s)	296, 529, 1894, 2015, 3178, 3543, 4350
BceAI	ACGGC	4 cut(s)	10, 162, 225, 1691
BciVI	GTATCC	4 cut(s)	1576, 3827, 3904, 5388
BclI	TGATCA	3 cut(s)	1045, 1237, 3868
BcnI	CCSGG	4 cut(s)	230, 231, 2103, 5295
BcoDI	GTCTC	8 cut(s)	194, 757, 1281, 1322, 2477, 2497, 4302, 4491
BfoI	RGCGCY	1 cut(s)	3167
BfrI	CTTAAG	2 cut(s)	947, 2213
BfuAI	ACCTGC	2 cut(s)	1660, 5044
BfuI	GTATCC	4 cut(s)	1576, 3827, 3904, 5388
BglI	GCCNNNNNGGC	1 cut(s)	218
BglII	AGATCT	1 cut(s)	3500
BisI	GCNGC	8 cut(s)	49, 310, 518, 1908, 2004, 3192, 3557, 4364
BlpI	GCTNAGC	2 cut(s)	513, 4466
BlsI	GCNGC	8 cut(s)	50, 311, 519, 1909, 2005, 3193, 3558, 4365
Bme18I	GGWCC	3 cut(s)	43, 1898, 2363
BmeT110I	CYCGRG	2 cut(s)	229, 789
BmgBI	CACGTC	4 cut(s)	713, 1879, 4611, 4632
BmgT120I	GGNCC	8 cut(s)	43, 900, 1145, 1146, 1898, 2363, 3378, 4175
BmiI	GGNNCC	6 cut(s)	245, 1147, 1148, 1491, 2094, 4293
BmrI	ACTGGG	6 cut(s)	1308, 1441, 1698, 1927, 2496, 5398
BmuI	ACTGGG	6 cut(s)	1308, 1441, 1698, 1927, 2496, 5398
BpiI	GAAGAC	2 cut(s)	149, 293
BplI	GAGNNNNNCTC	2 cut(s)	148, 180
BpmI	CTGGAG	9 cut(s)	269, 1718, 2111, 2951, 4326, 4343, 4398, 4536, 4683
Bpu10I	CCTNAGC	1 cut(s)	2978
Bpu1102I	GCTNAGC	2 cut(s)	513, 4466
Bpu14I	TTCGAA	2 cut(s)	3697, 4197
BpuMI	CCSGG	4 cut(s)	230, 231, 2103, 5295
BsaAI	YACGTR	1 cut(s)	1010
BsaBI	GATNNNNATC	2 cut(s)	2931, 3456
BsaHI	GRCGYC	1 cut(s)	2571
BsaI	GGTCTC	1 cut(s)	757
BsaWI	WCCGGW	3 cut(s)	183, 1826, 2707
Bse118I	RCCGGY	2 cut(s)	4026, 4172
Bse3DI	GCAATG	3 cut(s)	2770, 2852, 3615
Bse8I	GATNNNNATC	2 cut(s)	2931, 3456
BseAI	TCCGGA	2 cut(s)	1826, 2707
BseJI	GATNNNNATC	2 cut(s)	2931, 3456
BseMI	GCAATG	3 cut(s)	2770, 2852, 3615
BseRI	GAGGAG	6 cut(s)	362, 365, 777, 807, 2515, 3101
BseSI	GKGCMC	6 cut(s)	230, 246, 1149, 1344, 1494, 4318
BseXI	GCAGC	7 cut(s)	296, 529, 1894, 2015, 3178, 3543, 4350
BseYI	CCCAGC	2 cut(s)	127, 169
BsgI	GTGCAG	3 cut(s)	122, 3177, 3410
Bsh1236I	CGCG	4 cut(s)	51, 419, 3579, 4408
BshNI	GGYRCC	3 cut(s)	243, 1489, 4291
BsiHKAI	GWGCWC	7 cut(s)	114, 357, 514, 1482, 2185, 3470, 5350
BsiHKCI	CYCGRG	2 cut(s)	229, 789
BsiSI	CCGG	9 cut(s)	184, 230, 1827, 2102, 2708, 4027, 4173, 4721, 5295
BsiWI	CGTACG	1 cut(s)	1010
BsmAI	GTCTC	8 cut(s)	194, 757, 1281, 1322, 2477, 2497, 4302, 4491
BsmI	GAATGC	5 cut(s)	1969, 2890, 3031, 4351, 4532
Bso31I	GGTCTC	1 cut(s)	757
BsoBI	CYCGRG	2 cut(s)	229, 789
Bsp119I	TTCGAA	2 cut(s)	3697, 4197
Bsp120I	GGGCCC	1 cut(s)	1145
Bsp13I	TCCGGA	2 cut(s)	1826, 2707
Bsp1720I	GCTNAGC	2 cut(s)	513, 4466
Bsp19I	CCATGG	1 cut(s)	1061
Bsp68I	TCGCGA	1 cut(s)	3579
BspEI	TCCGGA	2 cut(s)	1826, 2707
BspFNI	CGCG	4 cut(s)	51, 419, 3579, 4408
BspHI	TCATGA	4 cut(s)	282, 1482, 1608, 3708
BspLI	GGNNCC	6 cut(s)	245, 1147, 1148, 1491, 2094, 4293
BspMAI	CTGCAG	1 cut(s)	2470
BspMI	ACCTGC	2 cut(s)	1660, 5044
BspQI	GCTCTTC	1 cut(s)	3813
BspT104I	TTCGAA	2 cut(s)	3697, 4197
BspT107I	GGYRCC	3 cut(s)	243, 1489, 4291
BspTI	CTTAAG	2 cut(s)	947, 2213
BspTNI	GGTCTC	1 cut(s)	757
BsrBI	CCGCTC	1 cut(s)	818
BsrDI	GCAATG	3 cut(s)	2770, 2852, 3615
BsrFI	RCCGGY	2 cut(s)	4026, 4172
BssAI	RCCGGY	2 cut(s)	4026, 4172
BssNI	GRCGYC	1 cut(s)	2571
BssSI	CACGAG	2 cut(s)	2160, 3102
BssT1I	CCWWGG	7 cut(s)	39, 896, 1061, 1921, 2037, 3369, 3399
Bst2BI	CACGAG	2 cut(s)	2160, 3102
BstACI	GRCGYC	1 cut(s)	2571
BstAFI	CTTAAG	2 cut(s)	947, 2213
BstBAI	YACGTR	1 cut(s)	1010
BstBI	TTCGAA	2 cut(s)	3697, 4197
BstC8I	GCNNGC	5 cut(s)	255, 421, 1912, 2233, 3213
BstDSI	CCRYGG	3 cut(s)	1061, 1673, 3551
BstENI	CCTNNNNNAGG	1 cut(s)	1158
BstFNI	CGCG	4 cut(s)	51, 419, 3579, 4408
BstH2I	RGCGCY	1 cut(s)	3167
BstHHI	GCGC	3 cut(s)	309, 1075, 3166
BstMAI	GTCTC	8 cut(s)	194, 757, 1281, 1322, 2477, 2497, 4302, 4491
BstNSI	RCATGY	1 cut(s)	2732
BstSLI	GKGCMC	6 cut(s)	230, 246, 1149, 1344, 1494, 4318
BstUI	CGCG	4 cut(s)	51, 419, 3579, 4408
BstV1I	GCAGC	7 cut(s)	296, 529, 1894, 2015, 3178, 3543, 4350
BstV2I	GAAGAC	2 cut(s)	149, 293
BstX2I	RGATCY	6 cut(s)	1027, 2737, 3457, 3484, 3500, 4070
BstXI	CCANNNNNNTGG	2 cut(s)	2089, 3248
BstYI	RGATCY	6 cut(s)	1027, 2737, 3457, 3484, 3500, 4070
BsuI	GTATCC	4 cut(s)	1576, 3827, 3904, 5388
BtgI	CCRYGG	3 cut(s)	1061, 1673, 3551
BtgZI	GCGATG	1 cut(s)	4389
BtrI	CACGTC	4 cut(s)	713, 1879, 4611, 4632
BtsI	GCAGTG	1 cut(s)	3398
BtsIMutI	CAGTG	7 cut(s)	121, 1940, 2832, 3155, 3398, 4422, 5303
BtuMI	TCGCGA	1 cut(s)	3579
BveI	ACCTGC	2 cut(s)	1660, 5044
Cac8I	GCNNGC	5 cut(s)	255, 421, 1912, 2233, 3213
CaiI	CAGNNNCTG	2 cut(s)	1769, 1907
CciI	TCATGA	4 cut(s)	282, 1482, 1608, 3708
CfoI	GCGC	3 cut(s)	309, 1075, 3166
Cfr10I	RCCGGY	2 cut(s)	4026, 4172
Cfr13I	GGNCC	8 cut(s)	43, 900, 1145, 1146, 1898, 2363, 3378, 4175
Cfr9I	CCCGGG	1 cut(s)	229
CseI	GACGC	4 cut(s)	40, 301, 425, 1259
CsiI	ACCWGGT	1 cut(s)	1159
Csp6I	GTAC	9 cut(s)	559, 1011, 1058, 1141, 1219, 1649, 2331, 3091, 5417
CviQI	GTAC	9 cut(s)	559, 1011, 1058, 1141, 1219, 1649, 2331, 3091, 5417
DraI	TTTAAA	2 cut(s)	486, 2902
DraIII	CACNNNGTG	1 cut(s)	3278
DrdI	GACNNNNNNGTC	2 cut(s)	295, 2370
DseDI	GACNNNNNNGTC	2 cut(s)	295, 2370
EaeI	YGGCCR	5 cut(s)	210, 255, 2849, 4126, 4137
EciI	GGCGGA	1 cut(s)	35
Ecl136II	GAGCTC	1 cut(s)	1480
Eco130I	CCWWGG	7 cut(s)	39, 896, 1061, 1921, 2037, 3369, 3399
Eco147I	AGGCCT	1 cut(s)	922
Eco24I	GRGCYC	5 cut(s)	74, 1149, 1482, 2161, 4472
Eco31I	GGTCTC	1 cut(s)	757
Eco32I	GATATC	1 cut(s)	3826
Eco47I	GGWCC	3 cut(s)	43, 1898, 2363
Eco53kI	GAGCTC	1 cut(s)	1480
Eco57I	CTGAAG	6 cut(s)	1728, 2888, 2896, 3375, 3774, 3791
Eco88I	CYCGRG	2 cut(s)	229, 789
EcoICRI	GAGCTC	1 cut(s)	1480
EcoNI	CCTNNNNNAGG	1 cut(s)	1158
EcoO109I	RGGNCCY	1 cut(s)	1146
EcoRI	GAATTC	2 cut(s)	2389, 3699
EcoRV	GATATC	1 cut(s)	3826
EcoT14I	CCWWGG	7 cut(s)	39, 896, 1061, 1921, 2037, 3369, 3399
EcoT22I	ATGCAT	4 cut(s)	1502, 1969, 2934, 5317
EcoT38I	GRGCYC	5 cut(s)	74, 1149, 1482, 2161, 4472
ErhI	CCWWGG	7 cut(s)	39, 896, 1061, 1921, 2037, 3369, 3399
FalI	AAGNNNNNCTT	4 cut(s)	3471, 3503, 4729, 4761
FauI	CCCGC	5 cut(s)	142, 1919, 4855, 4876, 4897
FauNDI	CATATG	2 cut(s)	1883, 2314
FbaI	TGATCA	3 cut(s)	1045, 1237, 3868
FblI	GTMKAC	1 cut(s)	3342
Fnu4HI	GCNGC	8 cut(s)	49, 310, 518, 1908, 2004, 3192, 3557, 4364
FriOI	GRGCYC	5 cut(s)	74, 1149, 1482, 2161, 4472
Fsp4HI	GCNGC	8 cut(s)	49, 310, 518, 1908, 2004, 3192, 3557, 4364
GlaI	GCGC	3 cut(s)	308, 1074, 3165
GluI	GCNGC	8 cut(s)	49, 310, 518, 1908, 2004, 3192, 3557, 4364
GsaI	CCCAGC	2 cut(s)	131, 173
GsuI	CTGGAG	9 cut(s)	269, 1718, 2111, 2951, 4326, 4343, 4398, 4536, 4683
HaeII	RGCGCY	1 cut(s)	3167
HapII	CCGG	9 cut(s)	184, 230, 1827, 2102, 2708, 4027, 4173, 4721, 5295
HgaI	GACGC	4 cut(s)	40, 301, 425, 1259
HhaI	GCGC	3 cut(s)	309, 1075, 3166
Hin1I	GRCGYC	1 cut(s)	2571
Hin6I	GCGC	3 cut(s)	307, 1073, 3164
HinP1I	GCGC	3 cut(s)	307, 1073, 3164
HincII	GTYRAC	4 cut(s)	1310, 2863, 3532, 3859
HindII	GTYRAC	4 cut(s)	1310, 2863, 3532, 3859
HpaI	GTTAAC	2 cut(s)	2863, 3859
HpaII	CCGG	9 cut(s)	184, 230, 1827, 2102, 2708, 4027, 4173, 4721, 5295
Hpy166II	GTNNAC	7 cut(s)	1310, 2303, 2863, 3343, 3532, 3859, 4081
Hpy8I	GTNNAC	7 cut(s)	1310, 2303, 2863, 3343, 3532, 3859, 4081
Hpy99I	CGWCG	1 cut(s)	80
Hsp92I	GRCGYC	1 cut(s)	2571
HspAI	GCGC	3 cut(s)	307, 1073, 3164
Kpn2I	TCCGGA	2 cut(s)	1826, 2707
Ksp22I	TGATCA	3 cut(s)	1045, 1237, 3868
KspAI	GTTAAC	2 cut(s)	2863, 3859
LguI	GCTCTTC	1 cut(s)	3813
LmnI	GCTCC	9 cut(s)	250, 332, 352, 1129, 1249, 1267, 1477, 1660, 2092
Lsp1109I	GCAGC	7 cut(s)	296, 529, 1894, 2015, 3178, 3543, 4350
MabI	ACCWGGT	1 cut(s)	1159
MbiI	CCGCTC	1 cut(s)	818
MfeI	CAATTG	3 cut(s)	177, 1992, 4296
MflI	RGATCY	6 cut(s)	1027, 2737, 3457, 3484, 3500, 4070
MlsI	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
MluI	ACGCGT	1 cut(s)	417
MluNI	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
MlyI	GAGTC	2 cut(s)	1096, 3146
MmeI	TCCRAC	8 cut(s)	944, 1455, 3063, 3888, 4703, 4736, 4820, 5114
Mox20I	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
Mph1103I	ATGCAT	4 cut(s)	1502, 1969, 2934, 5317
MroI	TCCGGA	2 cut(s)	1826, 2707
MroXI	GAANNNNTTC	2 cut(s)	597, 642
MscI	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
MslI	CAYNNNNRTG	6 cut(s)	1278, 1487, 1619, 3115, 3221, 3951
Msp20I	TGGCCA	4 cut(s)	257, 2851, 4128, 4139
MspA1I	CMGCKG	3 cut(s)	635, 2231, 4363
MspCI	CTTAAG	2 cut(s)	947, 2213
MspI	CCGG	9 cut(s)	184, 230, 1827, 2102, 2708, 4027, 4173, 4721, 5295
MunI	CAATTG	3 cut(s)	177, 1992, 4296
Mva1269I	GAATGC	5 cut(s)	1969, 2890, 3031, 4351, 4532
MvnI	CGCG	4 cut(s)	51, 419, 3579, 4408
NciI	CCSGG	4 cut(s)	230, 231, 2103, 5295
NcoI	CCATGG	1 cut(s)	1061
NdeI	CATATG	2 cut(s)	1883, 2314
NlaIV	GGNNCC	6 cut(s)	245, 1147, 1148, 1491, 2094, 4293
NmeAIII	GCCGAG	1 cut(s)	145
NmuCI	GTSAC	8 cut(s)	158, 233, 1099, 1390, 2830, 3138, 4449, 5187
NruI	TCGCGA	1 cut(s)	3579
NsiI	ATGCAT	4 cut(s)	1502, 1969, 2934, 5317
NspI	RCATGY	1 cut(s)	2732
NspV	TTCGAA	2 cut(s)	3697, 4197
PaeR7I	CTCGAG	1 cut(s)	789
PagI	TCATGA	4 cut(s)	282, 1482, 1608, 3708
PaqCI	CACCTGC	1 cut(s)	5044
PceI	AGGCCT	1 cut(s)	922
PciI	ACATGT	1 cut(s)	2728
PciSI	GCTCTTC	1 cut(s)	3813
PctI	GAATGC	5 cut(s)	1969, 2890, 3031, 4351, 4532
PdmI	GAANNNNTTC	2 cut(s)	597, 642
Pfl23II	CGTACG	1 cut(s)	1010
PflFI	GACNNNGTC	1 cut(s)	1292
PflMI	CCANNNNNTGG	3 cut(s)	688, 4135, 5391
PfoI	TCCNGGA	1 cut(s)	2967
PkrI	GCNGC	8 cut(s)	50, 311, 519, 1909, 2005, 3193, 3558, 4365
PleI	GAGTC	2 cut(s)	1096, 3145
PpsI	GAGTC	2 cut(s)	1096, 3145
Ppu21I	YACGTR	1 cut(s)	1010
PscI	ACATGT	1 cut(s)	2728
PshBI	ATTAAT	2 cut(s)	3303, 3473
PsiI	TTATAA	4 cut(s)	1110, 1260, 1404, 1712
Psp124BI	GAGCTC	1 cut(s)	1482
PspFI	CCCAGC	2 cut(s)	127, 169
PspLI	CGTACG	1 cut(s)	1010
PspN4I	GGNNCC	6 cut(s)	245, 1147, 1148, 1491, 2094, 4293
PspOMI	GGGCCC	1 cut(s)	1145
PspPI	GGNCC	8 cut(s)	43, 900, 1145, 1146, 1898, 2363, 3378, 4175
PspXI	VCTCGAGB	1 cut(s)	789
PstI	CTGCAG	1 cut(s)	2470
PstNI	CAGNNNCTG	2 cut(s)	1769, 1907
PsuI	RGATCY	6 cut(s)	1027, 2737, 3457, 3484, 3500, 4070
PsyI	GACNNNGTC	1 cut(s)	1292
PvuII	CAGCTG	2 cut(s)	2231, 4363
RruI	TCGCGA	1 cut(s)	3579
RsaI	GTAC	9 cut(s)	560, 1012, 1059, 1142, 1220, 1650, 2332, 3092, 5418
RsaNI	GTAC	9 cut(s)	559, 1011, 1058, 1141, 1219, 1649, 2331, 3091, 5417
RseI	CAYNNNNRTG	6 cut(s)	1278, 1487, 1619, 3115, 3221, 3951
SacI	GAGCTC	1 cut(s)	1482
SapI	GCTCTTC	1 cut(s)	3813
SatI	GCNGC	8 cut(s)	49, 310, 518, 1908, 2004, 3192, 3557, 4364
Sau96I	GGNCC	8 cut(s)	43, 900, 1145, 1146, 1898, 2363, 3378, 4175
SchI	GAGTC	2 cut(s)	1096, 3146
SexAI	ACCWGGT	1 cut(s)	1159
Sfr274I	CTCGAG	1 cut(s)	789
SfuI	TTCGAA	2 cut(s)	3697, 4197
SinI	GGWCC	3 cut(s)	43, 1898, 2363
SlaI	CTCGAG	1 cut(s)	789
SmaI	CCCGGG	1 cut(s)	231
SmiMI	CAYNNNNRTG	6 cut(s)	1278, 1487, 1619, 3115, 3221, 3951
SmlI	CTYRAG	3 cut(s)	789, 947, 2213
SmoI	CTYRAG	3 cut(s)	789, 947, 2213
SseBI	AGGCCT	1 cut(s)	922
SspI	AATATT	2 cut(s)	1114, 1665
SstI	GAGCTC	1 cut(s)	1482
StuI	AGGCCT	1 cut(s)	922
StyI	CCWWGG	7 cut(s)	39, 896, 1061, 1921, 2037, 3369, 3399
TaqI	TCGA	7 cut(s)	790, 1375, 2401, 2907, 3697, 3811, 4197
TatI	WGTACW	2 cut(s)	558, 3090
TauI	GCSGC	1 cut(s)	51
TscAI	CASTG	7 cut(s)	121, 1940, 2839, 3162, 3398, 4422, 5310
TseFI	GTSAC	8 cut(s)	158, 233, 1099, 1390, 2830, 3138, 4449, 5187
TseI	GCWGC	7 cut(s)	309, 517, 1907, 2003, 3191, 3556, 4363
Tsp45I	GTSAC	8 cut(s)	158, 233, 1099, 1390, 2830, 3138, 4449, 5187
TspGWI	ACGGA	4 cut(s)	823, 3540, 4259, 4731
TspMI	CCCGGG	1 cut(s)	229
TspRI	CASTG	7 cut(s)	121, 1940, 2839, 3162, 3398, 4422, 5310
Tth111I	GACNNNGTC	1 cut(s)	1292
Van91I	CCANNNNNTGG	3 cut(s)	688, 4135, 5391
Vha464I	CTTAAG	2 cut(s)	947, 2213
VpaK11BI	GGWCC	3 cut(s)	43, 1898, 2363
VspI	ATTAAT	2 cut(s)	3303, 3473
XagI	CCTNNNNNAGG	1 cut(s)	1158
XapI	RAATTY	8 cut(s)	408, 1916, 2389, 2557, 3543, 3638, 3699, 5356
XceI	RCATGY	1 cut(s)	2732
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1680
XhoI	CTCGAG	1 cut(s)	789
XmaI	CCCGGG	1 cut(s)	229
XmiI	GTMKAC	1 cut(s)	3342
XmnI	GAANNNNTTC	2 cut(s)	597, 642
ZraI	GACGTC	1 cut(s)	2572
Zsp2I	ATGCAT	4 cut(s)	1502, 1969, 2934, 5317

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.