Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Forward (+)

81962615 .. 81974532

Gene Structure 11918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00144060.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5766 bp

ATGGATTTCCGGTTCCCGTACTCTCCTCTGGAGGTTGCGACGCCGAGCTCCGTCCAGTTCGGCATCCTCAGCCCCGGCGAGATCGTAATTCTCCTTCTCACTGTTCTCTCGTTTTCCTTTTCTGATTATGTGTTTAGAAAAAGGAAACCGGAGATTTTGATACTGTTTGTGTGTGCTCTGAGGCAAATGTCGGTGCTGGAGGTCGAGAATGGCCGTTCAATGCTGAACGGGAAGCCGGTGAGAGGCGGCTTGAGTGATCCTCGGCTTGGTCCGATTGACAGGCACAACAGGTGTGAGACTTGTGCCAATAACTTGAGCGAGTGCCCGGGCCATTTCGGCCACATTGAGCTCGCCAAGCCTGTGTTTCACTTCGGGTTTATGAAGACTGTGCTGTCTGTAATGCGCTGCGTTTGCTTCAATTGCTCCAAACTTCTACTCGATGAGGAAGATGTTAAGTTTAAACAAGCTTTGAGAATCAAGAAGCCTAAAGATAGGCTAAAAAAGTTTGTGGATGCATGCAAAAACAAGACCAAGTGTGAAGGTGGTGATGAAATTGATCTGCAAGGCTCAAAATCCGGTCAACATGTAAAGCAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCCAAGTTCTCTATGGCTGGTTTGGAACTGGTTGCAGAGTACAAATCACGAAGGAAACAATATGATGAGGAACAGCTTCCTGAACCAGTGGAAAGGAAACAAATCCTTACTGCAGAAAAGGTTCTTAGCTTATTAAAGAGGATAAGTGATGAGGACTGCCAATTGTTGGGACTGAACCCTGAATATGCTCGACCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACCAGCTGTGAGACCCCCGGATGATTTAACACATCAGTTGTCCATGATTATTCGCCACAACGAGAATTTGAGGAAACAGGAAAAACATGGCTCAGCTGCTCATATCATTTCACAGTTTACACAAATCTTGCAATACTACATATCCACATATTTTGACAATAGCTTGCCTGGACTACCAAAGGCTGCTCATGGATCGACAAAGAGGCCTATTAAATCAATATGTTGTAGGCTTAAGGCAAAGCATGGCCGGATTAGAGGAAACTTGATGGGGAAGAGAGTCGATTTCTCAGCACGGACAGTTATTACACCAGATCCAAACCTTAGCCTTGATCAATTAGGAGTACCATGGAGCATTGCTATGAATCTCACATACACAGAGACTGTGACTCCATTTAATAAAGAAGGGTTGAAGGAGCTTGTTGAGTATGGGCCGCATCCCCCACCTGGACTAACTGGTGCAAAGTACATCATACGAGATGATGGCCAAAGACTCGATCTTCGTTACGTGGAGCGTCATCTAAAGGATGGTGACATTGTCCTCTTAAATCGTCAGCCTAGTCTCCATAAAATGTCATTCATGGGGCATAGAATCAAGATCATGCCATACTCAACTTTTCGCTTGAACTTATCTGTCACTTCACCTTACAATGCTGATTTTGATGGGGATGAAATGAACGTCCATGTTCCTCAGTCATTTGAAACTAGGGCAGAAGCTCTGGAGCTCATGATGGTGCCCAAATGCATTGTTTCCCCTCAGTCGAACCGGCCTGACGTTTTTATGAATATCTTGATGTGGTGGGAGGATTTTGACGGGAAAGTGCCTGCTCCTGCAATATTGAAGCCACGACCTCTTTGGACTGGGAAACAGGTTTTTAATCTTATAATACCGAAACAGATAAATCTCCAGAGAACTTCAGCGTGGCACTCTGAATCAGAAAGTGGTAACATTACCCCTGGTGATACTTTCGTTAGAATAGAAAAAGGGGAGTTACTTTCTGGAACTCTCTGTAAAAGGGCACTTGGGACTTCTACAGGAAGTCTTATACATGTCATATGGGAAGAGGTTGGTCCAGATGCAGCCTCCAAATTCCTTAGTAATACCCAGTGGCTTGTGAATTACTGGCTGTTGCAGGATGGTTTTAGCATTGGAATTGGAGATACAATTGCCGATGCAGCAACAATGGAAAAGATCAATGTAACTATTTACAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAGGCAGCTGGAGGCTGAACCAGGACGAACTATGATGGAATCCTTTGAAAACAAAGTGAACCAGGTGTTGAATAGGGCTCGTGATGATGCTGGAAGTAGTGCCCAGAAGAGTTTATCTGAGAGTAACAATCTCAAGGCCATGGTCACAGCTGGATCGAAGGGAAGTTTTATCAATATATCGCAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAGCGAATTCCGTATGGGTTCACAGACCGGACTTTGCCCCATTTTACGAAAGACGATTATGGACCGGAAAGTCGTGGCTTTGTAGAGAATTCCTACCTGCGTGGATTAACTCCACAGGAGTTTTATTTTCATGCCATGGGTGGTAGGGAAGGCATTATTGATACTGCAGTTAAGACATCTGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTTAAATATGATGGGACTGTCAGAAATTCATTGGGGGATGTAATACAGTTTCTCTATGGGGAAGATGGAATGGACGCTGTTTGGTTAGAATCACAAAAGCTGGATTCTTTAAAGATGAAAAAATCAGAATTTGACAAGACTTTCACTTTTGAGTTTGATGATAGAAATTGGAATCCGGACTATATATTGCCTGAATATGTTGAGGATCTAAAAACTATTGGAAAGTTTCGCTGGGCATTGGATGCTGAAGTTTCAAAACTTGAAGCTGATAGGATTCAACTTGGCACAGAGATTGCTGTCACAGGTGATAATACTTGGCCGTTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTCAAAATTGACTTCCGAAGGACTTCTGATATGCAACCAATGGAAATTATAGAAGCTATTGATAATCTTCAAGAGAGGCTGAAGGTTGTTCCTGGTGATGACTTGTTAAGTGTTGAAGCTCAAAATAATGCTACACTCTTTTTCAACATTTTGCTTCGCAGCACTTTTGCTAGCAAAAGGGTGCTAAAAGAGTACAAGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGGCACGCTTCCTGCAATCACTTGTAGCACCTGGGGAAATGATCGGTATTGTTGCTGCACAATCTATTGGCGAGCCTGCCACTCAGATGACTCTCAATACCTTCCACTATGCCGGTGTAAGTGCAAAGAATGTTACTCTAGGTGTTCCAAGGTTGAAGGAAATTATTGATGTCTCTAAGCGTATCAAAACACCATCTCTCTCTATCTACTTGAAGCCTGAAGCTAATAAAACCAAGGAGAAGGCCAAGAATGTTCAGTGTGCTTTGGAATACACTAACCTCGGAAGTGTTACTCAAGCTACAGAATTGTGGTATGATCCGGACCCTATGAGCACAATAATTGAAGAGGATCTTGAATTTGTCCAATCTTACTATGAAATGCCAGATGAAGATGTTAACCCTGAAAAAATATCACCTTGGTTGCTTCGCATAGAGATGAATCGTGAAATGATGGTGGATAAGAAACTGAGTATGGCTGACATTGCTGAGAAGATTAATTGTGAATATAGTGGTAAAATACAGTGCATCTTTAATGATGACAATTCTGAGAAGTTGATACTACGAATCCGTCTCATGAATGAAGAAGCTCCACATGAGGAACTGAATGATCAATCTGAAGATGATGTTTTCCTGAAGATGATTGAGAGTAACATGCTTACAAATATGGCTCTTCAAGGGATCCCAGAGATTAAAAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGGTTTAATGCAGAGCAAGAGTGGATGCTGGATACTGAAGGTGTTAATCTCTTAGCTGTCATGTGCCATGAGGATGTTGATGCCAAGAGGACAACAAGCAATGACTTGTATGAAATTATGGAAGTTCTTGGGATTGAGGCAGCTCGACGGGCATTGCTGGATGAGTTGCGTAATGTTATATCATTTGATGGATCCTATGTAAATTACCGGCACCTGGCCATTCTTTGTGATACCATGACCTATCGTGGCTACTTGATGGCTATCACTCGACATGGTATCAATCGAAAGGACACTGGTCCTATGATGCGATGCTCGTTTGAACAAACAGTGGACATGCTGCTGGATGCTTCAGTTTTTGCAGAAACAGATCATCTGAGGGGCGTAACAGAAAACATAATGCTAGGCAAGCTGGCACCAATTGGCACTGGAGACTGTGCCCTGTATCTGAATGAAGAGATGTTACAAAATGCTATGGAAGTGCAGCTGCCTGGTTATATGGATGGCCTTGATGTCGGCATGACACCATCCCATTCTCCATACCTTGAGAGCATACTGTCTCCTAGTTATTTGCCGAGTCCCAGTAATCCTCCTTCACCGATGTTGGATGCAGCGCAGTTTTCTCCATACGTTAGGGGAATTCCCTTTTCTCCTACTTCATCTCCAGGCAACATCCCATCATCTCTTCGCTACTATCCGCCATCCTTTGTATACAGTCCTACCTCCCCATACTATAGCCCCACGTCACCAGGTTATAGTCCATCCAGTCCCCCCTATTCTCCTTCATGTCCATCTTTCACATCACCAGGTTATACTCCAACCAGTCCTCCCTATTCTCCTTCACGTCCATCTTTCACATCACCAGGTTATAGTCCAACCAGTCCTGTCTATTCTCCTACAAGTCCATATTACTCACCGACCTCACCCAACTATAGCCCTATCTCTCCAACATCAACTCCTTATAGCCCAACTTCACCTGCATACGACCGAACACCACCGCCTTATGGCCTGACTTTACCTGCATATAGTCCCACTTCTCCTTTGTACACGCCAACATCACCCTCCTACAGCCCAACCTCTCCAAGCTACAGCCCGACTTTCCCCACATACACCCCCTCTTCGCTTTCCTACAGCCCCACATCGCCATCATACAGCCCCACATCGCCCTCCTACAGCCCCACATCGCCATCATACAGCCCTACATCACCCTCCTACAGGCCTAGCTCCCCATACGATAGCCTGACATCTCCAGGTTACAACCCAACCTCTCCCAGTTATAGCCTGACATCGCTGTACAGCGCAACTTCACTAGCATATTCCCCAACATCTCCAACTTACTCTCCATCGAGCCCCACTTACAGTCCCAGCAGCCTATTCACCACCTCTGCAGTGAGCCCAGACTATTCCCCAAGTGGTCCACAGTACAGAGATGCGGAGAGGAATGTGGTGTCGTGGACTTCGATCTTGTGTGGGTTAGCTTGTACGGGGAGGATTGAGGAGGCGAGGCGGTTGTTTGAGGAAGTGCCGGAGAGGAATGTTGTTTCTTGGAATTCTATGATTCTTGGGTTGGTTAAGAATGGGGACTTGGAGGGAGCACGAGAGGTTTTTGACCGGATGCGGATGAAGAATGTTGTTTCTTGTAATGCTATGATTGGTGGGTATGCGGAGTATTGTAGAATGGATGAGGCGAGAAGTTTGTTTCATGGGATGCTAGATAGGAATGTGATTACTTGGACTAGCATGATATCTAGGTATTGTAGGTCCGGCAATGTGGATGAAGGGCCGGTATGCTTTGTTTCAAAAAATGCACGAGAGGAACGTTGTTTCTTGGACAACCATGATTGGTGGCTTTGCTCGGAATGGATTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1921

Amino Acids

215.73

Weight (kDa)

5.78

Isoelectric Point (pI)

55.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	60 - 372	5.9e-88	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	374 - 528	6.2e-61	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	540 - 671	1.1e-35	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	698 - 802	1.2e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	809 - 1398	7.6e-106	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	875 - 1059	9.2e-65	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1144 - 1277	2.8e-50	RNA polymerase Rpb1, domain 7
PPR_2	PF13041	1809 - 1842	1.5e-06	PPR repeat family
PPR	PF01535	1812 - 1842	9.7e-08	PPR repeat
PPR_2	PF13041	1840 - 1885	2.2e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1733
AarI	CACCTGC	1 cut(s)	4879
AasI	GACNNNNNNGTC	2 cut(s)	391, 2391
Acc36I	ACCTGC	3 cut(s)	2427, 4879, 4921
AccB1I	GGYRCC	3 cut(s)	1582, 4107, 4309
AccB7I	CCANNNNNTGG	1 cut(s)	787
AccI	GTMKAC	1 cut(s)	4605
AccIII	TCCGGA	2 cut(s)	2728, 3475
AciI	CCGC	8 cut(s)	246, 1283, 4592, 4892, 5327, 5401, 5512, 5558
AclI	AACGTT	1 cut(s)	5713
AcoI	YGGCCR	6 cut(s)	211, 337, 1096, 1333, 2870, 4113
AcsI	RAATTY	9 cut(s)	916, 1937, 2328, 2410, 2578, 2681, 3512, 4533, 5443
AcuI	CTGAAG	9 cut(s)	1749, 2820, 2909, 3020, 3396, 3792, 3809, 3954, 4230
AcyI	GRCGYC	1 cut(s)	41
AfaI	GTAC	9 cut(s)	20, 662, 1194, 1316, 3113, 4940, 5189, 5318, 5377
AfiI	CCNNNNNNNGG	8 cut(s)	242, 266, 787, 1295, 2103, 4111, 4898, 5109
AflII	CTTAAG	1 cut(s)	1082
AflIII	ACRYGT	2 cut(s)	583, 1897
AjiI	CACGTC	2 cut(s)	4638, 4739
AjuI	GAANNNNNNNTTGG	2 cut(s)	5462, 5494
AloI	GAACNNNNNNTCC	1 cut(s)	28
Alw21I	GWGCWC	7 cut(s)	50, 178, 351, 616, 1575, 3491, 5491
AlwNI	CAGNNNCTG	1 cut(s)	1232
Ama87I	CYCGRG	1 cut(s)	325
Aor13HI	TCCGGA	2 cut(s)	2728, 3475
ApoI	RAATTY	9 cut(s)	916, 1937, 2328, 2410, 2578, 2681, 3512, 4533, 5443
ArsI	GACNNNNNNTTYG	2 cut(s)	1332, 1364
AseI	ATTAAT	1 cut(s)	3651
Asp700I	GAANNNNTTC	3 cut(s)	696, 741, 2941
AspLEI	GCGC	3 cut(s)	405, 4510, 5195
AsuC2I	CCSGG	4 cut(s)	75, 326, 327, 869
AsuNHI	GCTAGC	1 cut(s)	3089
AvaI	CYCGRG	1 cut(s)	325
AvaII	GGWCC	7 cut(s)	269, 1919, 2384, 3478, 4193, 5309, 5655
BaeGI	GKGCMC	5 cut(s)	326, 1587, 1870, 2206, 4336
BaeI	ACNNNNGTAYC	4 cut(s)	1803, 1836, 2475, 2508
BalI	TGGCCA	2 cut(s)	1335, 4115
BamHI	GGATCC	2 cut(s)	3834, 4088
BanI	GGYRCC	3 cut(s)	1582, 4107, 4309
BanII	GRGCYC	6 cut(s)	50, 351, 1575, 2182, 5246, 5291
BarI	GAAGNNNNNNTAC	6 cut(s)	78, 110, 4341, 4373, 4471, 4503
BauI	CACGAG	4 cut(s)	2181, 3123, 5490, 5703
BbsI	GAAGAC	1 cut(s)	389
Bbv12I	GWGCWC	7 cut(s)	50, 178, 351, 616, 1575, 3491, 5491
BbvCI	CCTCAGC	1 cut(s)	68
BceAI	ACGGC	2 cut(s)	198, 2857
BciVI	GTATCC	1 cut(s)	3922
BclI	TGATCA	3 cut(s)	1180, 3763, 3886
BcnI	CCSGG	4 cut(s)	75, 326, 327, 869
BfrI	CTTAAG	1 cut(s)	1082
BfuAI	ACCTGC	3 cut(s)	2427, 4879, 4921
BfuI	GTATCC	1 cut(s)	3922
BglI	GCCNNNNNGGC	1 cut(s)	336
BlpI	GCTNAGC	2 cut(s)	615, 943
Bme18I	GGWCC	7 cut(s)	269, 1919, 2384, 3478, 4193, 5309, 5655
BmeT110I	CYCGRG	1 cut(s)	325
BmgBI	CACGTC	2 cut(s)	4638, 4739
BmiI	GGNNCC	7 cut(s)	14, 1584, 3480, 3836, 4090, 4109, 4311
BmrI	ACTGGG	5 cut(s)	1719, 1948, 2517, 4470, 5160
BmtI	GCTAGC	1 cut(s)	3093
BmuI	ACTGGG	5 cut(s)	1719, 1948, 2517, 4470, 5160
BoxI	GACNNNNGTC	1 cut(s)	4191
BpiI	GAAGAC	1 cut(s)	389
BplI	GAGNNNNNCTC	2 cut(s)	244, 276
BpmI	CTGGAG	8 cut(s)	50, 218, 1589, 1739, 2132, 4344, 4542, 5127
Bpu10I	CCTNAGC	2 cut(s)	68, 1172
Bpu1102I	GCTNAGC	2 cut(s)	615, 943
BpuEI	CTTGAG	5 cut(s)	271, 334, 2219, 3435, 4460
BpuMI	CCSGG	4 cut(s)	75, 326, 327, 869
BsaAI	YACGTR	1 cut(s)	1357
BsaBI	GATNNNNATC	1 cut(s)	4157
BsaHI	GRCGYC	1 cut(s)	41
BsaI	GGTCTC	1 cut(s)	856
BsaWI	WCCGGW	8 cut(s)	9, 148, 575, 2349, 2386, 2728, 3475, 5505
Bsc4I	CCNNNNNNNGG	8 cut(s)	242, 266, 787, 1295, 2103, 4111, 4898, 5109
Bse118I	RCCGGY	5 cut(s)	235, 1614, 3269, 4104, 5677
Bse3DI	GCAATG	5 cut(s)	1203, 3636, 4003, 4049, 5668
Bse8I	GATNNNNATC	1 cut(s)	4157
BseAI	TCCGGA	2 cut(s)	2728, 3475
BseJI	GATNNNNATC	1 cut(s)	4157
BseLI	CCNNNNNNNGG	8 cut(s)	242, 266, 787, 1295, 2103, 4111, 4898, 5109
BseMI	GCAATG	5 cut(s)	1203, 3636, 4003, 4049, 5668
BseRI	GAGGAG	3 cut(s)	15, 2536, 5405
BseSI	GKGCMC	5 cut(s)	326, 1587, 1870, 2206, 4336
BseYI	CCCAGC	2 cut(s)	2784, 5258
BsgI	GTGCAG	2 cut(s)	3198, 4397
Bsh1285I	CGRYCG	1 cut(s)	4882
BshNI	GGYRCC	3 cut(s)	1582, 4107, 4309
BsiEI	CGRYCG	1 cut(s)	4882
BsiHKAI	GWGCWC	7 cut(s)	50, 178, 351, 616, 1575, 3491, 5491
BsiHKCI	CYCGRG	1 cut(s)	325
BslFI	GGGAC	9 cut(s)	804, 1888, 2316, 2581, 4458, 4647, 4908, 5241, 5489
BslI	CCNNNNNNNGG	8 cut(s)	242, 266, 787, 1295, 2103, 4111, 4898, 5109
BsmBI	CGTCTC	1 cut(s)	3731
BsmFI	GGGAC	9 cut(s)	804, 1888, 2316, 2581, 4458, 4647, 4908, 5241, 5489
BsmI	GAATGC	1 cut(s)	2911
Bso31I	GGTCTC	1 cut(s)	856
BsoBI	CYCGRG	1 cut(s)	325
Bsp13I	TCCGGA	2 cut(s)	2728, 3475
Bsp1407I	TGTACA	2 cut(s)	4938, 5187
Bsp1720I	GCTNAGC	2 cut(s)	615, 943
Bsp19I	CCATGG	3 cut(s)	1196, 2241, 2457
BspACI	CCGC	8 cut(s)	246, 1283, 4592, 4892, 5327, 5401, 5512, 5558
BspEI	TCCGGA	2 cut(s)	2728, 3475
BspHI	TCATGA	2 cut(s)	1575, 3729
BspLI	GGNNCC	7 cut(s)	14, 1584, 3480, 3836, 4090, 4109, 4311
BspMAI	CTGCAG	3 cut(s)	736, 2491, 5284
BspMI	ACCTGC	3 cut(s)	2427, 4879, 4921
BspOI	GCTAGC	1 cut(s)	3093
BspQI	GCTCTTC	1 cut(s)	3831
BspT107I	GGYRCC	3 cut(s)	1582, 4107, 4309
BspTI	CTTAAG	1 cut(s)	1082
BspTNI	GGTCTC	1 cut(s)	856
BsrDI	GCAATG	5 cut(s)	1203, 3636, 4003, 4049, 5668
BsrFI	RCCGGY	5 cut(s)	235, 1614, 3269, 4104, 5677
BsrGI	TGTACA	2 cut(s)	4938, 5187
BssAI	RCCGGY	5 cut(s)	235, 1614, 3269, 4104, 5677
BssNAI	GTATAC	1 cut(s)	4606
BssNI	GRCGYC	1 cut(s)	41
BssSI	CACGAG	4 cut(s)	2181, 3123, 5490, 5703
BssT1I	CCWWGG	6 cut(s)	1196, 2241, 2457, 3305, 3390, 3572
Bst1107I	GTATAC	1 cut(s)	4606
Bst2BI	CACGAG	4 cut(s)	2181, 3123, 5490, 5703
Bst6I	CTCTTC	9 cut(s)	1118, 1905, 2204, 2885, 3495, 3831, 4344, 4584, 5017
BstACI	GRCGYC	1 cut(s)	41
BstAFI	CTTAAG	1 cut(s)	1082
BstAPI	GCANNNNNTGC	1 cut(s)	2795
BstAUI	TGTACA	2 cut(s)	4938, 5187
BstBAI	YACGTR	1 cut(s)	1357
BstDSI	CCRYGG	3 cut(s)	1196, 2241, 2457
BstENI	CCTNNNNNAGG	1 cut(s)	5107
BstHHI	GCGC	3 cut(s)	405, 4510, 5195
BstMCI	CGRYCG	1 cut(s)	4882
BstNSI	RCATGY	5 cut(s)	519, 587, 1901, 3811, 4234
BstPAI	GACNNNNGTC	1 cut(s)	4191
BstSLI	GKGCMC	5 cut(s)	326, 1587, 1870, 2206, 4336
BstV2I	GAAGAC	1 cut(s)	389
BstX2I	RGATCY	5 cut(s)	1162, 2758, 3505, 3834, 4088
BstXI	CCANNNNNNTGG	1 cut(s)	2110
BstYI	RGATCY	5 cut(s)	1162, 2758, 3505, 3834, 4088
BstZ17I	GTATAC	1 cut(s)	4606
BsuI	GTATCC	1 cut(s)	3922
BtgI	CCRYGG	3 cut(s)	1196, 2241, 2457
BtgZI	GCGATG	5 cut(s)	4219, 5019, 5040, 5061, 5166
BtrI	CACGTC	2 cut(s)	4638, 4739
BtsI	GCAGTG	1 cut(s)	5289
BtsIMutI	CAGTG	9 cut(s)	99, 714, 1961, 3419, 3683, 4188, 4230, 4320, 5289
BveI	ACCTGC	3 cut(s)	2427, 4879, 4921
CaiI	CAGNNNCTG	1 cut(s)	1232
CciI	TCATGA	2 cut(s)	1575, 3729
CfoI	GCGC	3 cut(s)	405, 4510, 5195
Cfr10I	RCCGGY	5 cut(s)	235, 1614, 3269, 4104, 5677
Cfr9I	CCCGGG	1 cut(s)	325
CseI	GACGC	3 cut(s)	49, 1352, 2636
CsiI	ACCWGGT	4 cut(s)	2163, 4642, 4699, 4756
Csp6I	GTAC	9 cut(s)	19, 661, 1193, 1315, 3112, 4939, 5188, 5317, 5376
CspCI	CAANNNNNGTGG	2 cut(s)	985, 1020
CviQI	GTAC	9 cut(s)	19, 661, 1193, 1315, 3112, 4939, 5188, 5317, 5376
DraI	TTTAAA	2 cut(s)	460, 2664
DrdI	GACNNNNNNGTC	2 cut(s)	391, 2391
DseDI	GACNNNNNNGTC	2 cut(s)	391, 2391
EaeI	YGGCCR	6 cut(s)	211, 337, 1096, 1333, 2870, 4113
Eam1104I	CTCTTC	9 cut(s)	1118, 1905, 2204, 2885, 3495, 3831, 4344, 4584, 5017
EarI	CTCTTC	9 cut(s)	1118, 1905, 2204, 2885, 3495, 3831, 4344, 4584, 5017
EciI	GGCGGA	1 cut(s)	4581
Ecl136II	GAGCTC	3 cut(s)	48, 349, 1573
Eco130I	CCWWGG	6 cut(s)	1196, 2241, 2457, 3305, 3390, 3572
Eco147I	AGGCCT	2 cut(s)	1057, 5113
Eco24I	GRGCYC	6 cut(s)	50, 351, 1575, 2182, 5246, 5291
Eco31I	GGTCTC	1 cut(s)	856
Eco32I	GATATC	1 cut(s)	5640
Eco47I	GGWCC	7 cut(s)	269, 1919, 2384, 3478, 4193, 5309, 5655
Eco53kI	GAGCTC	3 cut(s)	48, 349, 1573
Eco57I	CTGAAG	9 cut(s)	1749, 2820, 2909, 3020, 3396, 3792, 3809, 3954, 4230
Eco88I	CYCGRG	1 cut(s)	325
EcoICRI	GAGCTC	3 cut(s)	48, 349, 1573
EcoNI	CCTNNNNNAGG	1 cut(s)	5107
EcoRI	GAATTC	4 cut(s)	2328, 2410, 4533, 5443
EcoRV	GATATC	1 cut(s)	5640
EcoT14I	CCWWGG	6 cut(s)	1196, 2241, 2457, 3305, 3390, 3572
EcoT22I	ATGCAT	2 cut(s)	517, 1595
EcoT38I	GRGCYC	6 cut(s)	50, 351, 1575, 2182, 5246, 5291
ErhI	CCWWGG	6 cut(s)	1196, 2241, 2457, 3305, 3390, 3572
Esp3I	CGTCTC	1 cut(s)	3731
FalI	AAGNNNNNCTT	2 cut(s)	3492, 3524
FaqI	GGGAC	9 cut(s)	804, 1888, 2316, 2581, 4458, 4647, 4908, 5241, 5489
FauNDI	CATATG	1 cut(s)	1904
FbaI	TGATCA	3 cut(s)	1180, 3763, 3886
FblI	GTMKAC	1 cut(s)	4605
FriOI	GRGCYC	6 cut(s)	50, 351, 1575, 2182, 5246, 5291
GlaI	GCGC	3 cut(s)	404, 4509, 5194
GsaI	CCCAGC	2 cut(s)	2788, 5262
GsuI	CTGGAG	8 cut(s)	50, 218, 1589, 1739, 2132, 4344, 4542, 5127
HgaI	GACGC	3 cut(s)	49, 1352, 2636
HhaI	GCGC	3 cut(s)	405, 4510, 5195
Hin1I	GRCGYC	1 cut(s)	41
Hin6I	GCGC	3 cut(s)	403, 4508, 5193
HinP1I	GCGC	3 cut(s)	403, 4508, 5193
HincII	GTYRAC	4 cut(s)	581, 2884, 3553, 3877
HindII	GTYRAC	4 cut(s)	581, 2884, 3553, 3877
HindIII	AAGCTT	2 cut(s)	465, 3116
HpaI	GTTAAC	3 cut(s)	2884, 3553, 3877
Hpy99I	CGWCG	2 cut(s)	43, 4047
HpyCH4IV	ACGT	7 cut(s)	1356, 1527, 1623, 4524, 4637, 4738, 5713
HpySE526I	ACGT	7 cut(s)	1356, 1527, 1623, 4524, 4637, 4738, 5713
Hsp92I	GRCGYC	1 cut(s)	41
HspAI	GCGC	3 cut(s)	403, 4508, 5193
Kpn2I	TCCGGA	2 cut(s)	2728, 3475
Ksp22I	TGATCA	3 cut(s)	1180, 3763, 3886
KspAI	GTTAAC	3 cut(s)	2884, 3553, 3877
LguI	GCTCTTC	1 cut(s)	3831
MabI	ACCWGGT	4 cut(s)	2163, 4642, 4699, 4756
MaeII	ACGT	7 cut(s)	1356, 1527, 1623, 4524, 4637, 4738, 5713
MfeI	CAATTG	4 cut(s)	418, 782, 2013, 4314
MflI	RGATCY	5 cut(s)	1162, 2758, 3505, 3834, 4088
MlsI	TGGCCA	2 cut(s)	1335, 4115
MluNI	TGGCCA	2 cut(s)	1335, 4115
MlyI	GAGTC	5 cut(s)	1137, 1231, 1335, 3241, 4480
MmeI	TCCRAC	5 cut(s)	4479, 4736, 4793, 4865, 5249
Mox20I	TGGCCA	2 cut(s)	1335, 4115
Mph1103I	ATGCAT	2 cut(s)	517, 1595
MroI	TCCGGA	2 cut(s)	2728, 3475
MroXI	GAANNNNTTC	3 cut(s)	696, 741, 2941
MscI	TGGCCA	2 cut(s)	1335, 4115
MslI	CAYNNNNRTG	6 cut(s)	1208, 1580, 3136, 3242, 3590, 3969
Msp20I	TGGCCA	2 cut(s)	1335, 4115
MspA1I	CMGCKG	5 cut(s)	857, 947, 2110, 2252, 4381
MspCI	CTTAAG	1 cut(s)	1082
MssI	GTTTAAAC	1 cut(s)	460
MunI	CAATTG	4 cut(s)	418, 782, 2013, 4314
Mva1269I	GAATGC	1 cut(s)	2911
NciI	CCSGG	4 cut(s)	75, 326, 327, 869
NcoI	CCATGG	3 cut(s)	1196, 2241, 2457
NdeI	CATATG	1 cut(s)	1904
NheI	GCTAGC	1 cut(s)	3089
NlaIV	GGNNCC	7 cut(s)	14, 1584, 3480, 3836, 4090, 4109, 4311
NmeAIII	GCCGAG	3 cut(s)	69, 241, 4494
NmuCI	GTSAC	6 cut(s)	1234, 1379, 1483, 2245, 2851, 4638
NsiI	ATGCAT	2 cut(s)	517, 1595
NspI	RCATGY	5 cut(s)	519, 587, 1901, 3811, 4234
PaeI	GCATGC	1 cut(s)	519
PagI	TCATGA	2 cut(s)	1575, 3729
PaqCI	CACCTGC	1 cut(s)	4879
PceI	AGGCCT	2 cut(s)	1057, 5113
PciI	ACATGT	2 cut(s)	583, 1897
PciSI	GCTCTTC	1 cut(s)	3831
PcsI	WCGNNNNNNNCGW	2 cut(s)	5351, 5710
PctI	GAATGC	1 cut(s)	2911
PdmI	GAANNNNTTC	3 cut(s)	696, 741, 2941
PflFI	GACNNNGTC	1 cut(s)	1385
PflMI	CCANNNNNTGG	1 cut(s)	787
PleI	GAGTC	5 cut(s)	1136, 1231, 1335, 3241, 4479
PmeI	GTTTAAAC	1 cut(s)	460
PpsI	GAGTC	5 cut(s)	1136, 1231, 1335, 3241, 4479
Ppu21I	YACGTR	1 cut(s)	1357
PscI	ACATGT	2 cut(s)	583, 1897
PshAI	GACNNNNGTC	1 cut(s)	4191
PshBI	ATTAAT	1 cut(s)	3651
PsiI	TTATAA	1 cut(s)	1733
Psp124BI	GAGCTC	3 cut(s)	50, 351, 1575
Psp1406I	AACGTT	1 cut(s)	5713
PspFI	CCCAGC	2 cut(s)	2784, 5258
PspN4I	GGNNCC	7 cut(s)	14, 1584, 3480, 3836, 4090, 4109, 4311
PstI	CTGCAG	3 cut(s)	736, 2491, 5284
PstNI	CAGNNNCTG	1 cut(s)	1232
PsuI	RGATCY	5 cut(s)	1162, 2758, 3505, 3834, 4088
PsyI	GACNNNGTC	1 cut(s)	1385
PvuII	CAGCTG	5 cut(s)	857, 947, 2110, 2252, 4381
RsaI	GTAC	9 cut(s)	20, 662, 1194, 1316, 3113, 4940, 5189, 5318, 5377
RsaNI	GTAC	9 cut(s)	19, 661, 1193, 1315, 3112, 4939, 5188, 5317, 5376
RseI	CAYNNNNRTG	6 cut(s)	1208, 1580, 3136, 3242, 3590, 3969
SacI	GAGCTC	3 cut(s)	50, 351, 1575
SapI	GCTCTTC	1 cut(s)	3831
SchI	GAGTC	5 cut(s)	1137, 1231, 1335, 3241, 4480
SexAI	ACCWGGT	4 cut(s)	2163, 4642, 4699, 4756
SfiI	GGCCNNNNNGGCC	1 cut(s)	336
SinI	GGWCC	7 cut(s)	269, 1919, 2384, 3478, 4193, 5309, 5655
SmaI	CCCGGG	1 cut(s)	327
SmiMI	CAYNNNNRTG	6 cut(s)	1208, 1580, 3136, 3242, 3590, 3969
SmlI	CTYRAG	6 cut(s)	250, 313, 1082, 2234, 3450, 4439
SmoI	CTYRAG	6 cut(s)	250, 313, 1082, 2234, 3450, 4439
SphI	GCATGC	1 cut(s)	519
SrfI	GCCCGGGC	1 cut(s)	327
SseBI	AGGCCT	2 cut(s)	1057, 5113
SsiI	CCGC	8 cut(s)	246, 1283, 4592, 4892, 5327, 5401, 5512, 5558
SspI	AATATT	1 cut(s)	1686
SstI	GAGCTC	3 cut(s)	50, 351, 1575
StuI	AGGCCT	2 cut(s)	1057, 5113
StyI	CCWWGG	6 cut(s)	1196, 2241, 2457, 3305, 3390, 3572
TaiI	ACGT	7 cut(s)	1359, 1530, 1626, 4527, 4640, 4741, 5716
TaqII	GACCGA	1 cut(s)	4896
TatI	WGTACW	6 cut(s)	660, 1314, 3111, 4938, 5187, 5316
TauI	GCSGC	2 cut(s)	249, 1285
TscAI	CASTG	9 cut(s)	106, 714, 1961, 3419, 3683, 4195, 4230, 4327, 5289
TseFI	GTSAC	6 cut(s)	1234, 1379, 1483, 2245, 2851, 4638
Tsp45I	GTSAC	6 cut(s)	1234, 1379, 1483, 2245, 2851, 4638
TspGWI	ACGGA	4 cut(s)	40, 1159, 2322, 3713
TspMI	CCCGGG	1 cut(s)	325
TspRI	CASTG	9 cut(s)	106, 714, 1961, 3419, 3683, 4195, 4230, 4327, 5289
Tth111I	GACNNNGTC	1 cut(s)	1385
Van91I	CCANNNNNTGG	1 cut(s)	787
Vha464I	CTTAAG	1 cut(s)	1082
VpaK11BI	GGWCC	7 cut(s)	269, 1919, 2384, 3478, 4193, 5309, 5655
VspI	ATTAAT	1 cut(s)	3651
XagI	CCTNNNNNAGG	1 cut(s)	5107
XapI	RAATTY	9 cut(s)	916, 1937, 2328, 2410, 2578, 2681, 3512, 4533, 5443
XceI	RCATGY	5 cut(s)	519, 587, 1901, 3811, 4234
XcmI	CCANNNNNNNNNTGG	2 cut(s)	631, 1701
XmaI	CCCGGG	1 cut(s)	325
XmiI	GTMKAC	1 cut(s)	4605
XmnI	GAANNNNTTC	3 cut(s)	696, 741, 2941
Zsp2I	ATGCAT	2 cut(s)	517, 1595

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.