Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Forward (+)

81976989 .. 82034587

Gene Structure 57599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00144070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5340 bp

ATGAAGTGTGAGACTTGCACGGCCAACATGGCTGAGTGCCCGGGTCACTTTGGGCACCTGGAGCTGGCTAAGCCCATGTTTCATATTGGGTTCATGAAGACTGTGTTGTCTATAATGCGCTGCGTCTGCTTCAACTGCTCCAAAATCCTAGTTGATGAGGAGGACCACAAGTTTAAGACAGCTTTGAGAATCAAGAACCCAAAAAATAGGCTAAAGAAAATTTTGGACGCGTGCAAAAACAAAACCAAGTGTGAAGGCGGTGATGAGATTGATGTGCAAGAATCGGATGAACCTTTAAAGAAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCGAAGCTCTCTATAGATGGTATGAAGATGGTTGCAGAGTACAAAGCCCAAAGAAAGAAGAGTGATGACCAGGAACAGCTTCCTGAACCTGTGGAAAGGAAGCAGACCCTTACCGCTGAAAGGGTTCTTAGTGTGTTGAAGAGGATAAGTGATGAAGATTGCCAATTATTGGGATTGAACCCCAAGTATGCACGGCCAGATTGGATGATTTTACAAGTTCTTCCCATTCCTCCACAACCTGTGAGACCCTCTGTGATGATGGACACCTCCTCGAGGAGTGAGGATGATTTAACACATCAGCTGGCCATGATTATCCGCCACAACGAGAATCTGAGGAGACAGGAAAGAAACGGATCACCCGCTCATATCATCTCAGAGTTTGCACAACTCTTGCAATTCCATATAGCTACATATTTTGATAATGAGTTGCCTGGACTACCAAGGGCCACACAGAGATCAGGGAGGCCTATTAAATCAATTTGTAGTAGGCTTAAGGCGAAGGAAGGTCGGATTAGAGGAAACTTAATGGGGAAGAGGGTTGATTTCTCAGCACGTACGGTTATTACACCAGATCCGAACATTAACATTGATCAATTAGGAGTACCATGGAGTATTGCGTTGAATCTCACATACCCTGAAACTGTGACTCCTTATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTACGGGCCCCATCCTCCACCAGGTAAAACTGGTGCAAAGTATATAATAAGAGATGATGGGCAAAGGCTTGATCTACGGTACTTGAAGAAAAGTAGTGATCATCATTTGGAGCTTGGTTATAAGGTGGAGCGTCATTTGAATGATGGAGACTTTGTCCTTTTTAATCGTCAACCCAGTCTCCATAAAATGTCTATTATGGGGCACAGAATCAAAATCATGCCTTACTCAACTTTTCGATTAAATCTATCTGTCACTTCTCCTTATAATGCTGATTTTGATGGGGATGAAATGAATATGCACGTTCCCCAGTCATTTGAAACTAGGGCAGAAGTGTTGGAGCTCATGATGGTGCCCAAATGCATTGTGTCCCCTCAATCAAATCGTCCTGTGATGGGAATAGTCCAAGATACCCTGTTGGGTTGTCGGAAGATCACCAAGAGGGATACCTTCATAGAGAAGGATGTTTTCATGAACATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTACCTGCTCCTGCAATATTGAAGCCACGGCCTCTTTGGACTGGGAAACAGGTGTTTAATCTTATAATACCAAAGCAAATAAATCTCCAGAGAACTTCAGCGTGGCATTCTGAGTCAGAAACTGGTAACATTACCCCTGGTGATACTCACGTTAGAATAGAAAAAGGGGAGTTACTTTCTGGAACTCTGTGCAAAAAGGCACTTGGGACATCTACTGGAAGTCTTATACACGTCATATGGGAAGAGGTTGGTCCAGATGCTGCCCGCAAATTCCTTGGTCATACCCAGTGGCTTGTGAATTACTGGCTGTTGCAAAATGCATTTAGCATTGGAATTGGAGATACAATTGCTGATGCAGCAACGATGGAAAAGATCAATGAAACTATTTCCAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAAGAGCCTGGAGCCTGAACCGGGGCGAACTATGATGGATTCCTTTGAAAACAAAGTGAATCAGGTCTTAAATAGGGCTCGTGATGATGCTGGAAGTAGTGCTCAGAAGAGTTTGTCTGAGAGTAACAATCTTAAGGCTATGGTTACAGCTGGCTCTAAGGGAAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAACGAATCCCATATGGGTTCATAGACCGTACTTTGCCCCACTTCACTAAGGACGACTATGGACCAGAAAGTCGTGGCTTTGTAGAGAATTCATACCTTCGAGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGAGGTAGGGAAGGTCTTATAGATACTGCAGTTAAGACATCCGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTCAAATATGATGGGACTGTGAGAAATTCGTTGGGGGACGTCATACAGTTTCTCTATGGAGAAGATGGAATGGATGCTGTATGGATAGAATCACAAAAGCTGGATTCTCTTAAAATGAAGAAGAAAGAGTTTGACAAGACTTTCGCCTATGTGTTTGATGATGAAAATTGGAATCCGGACTATATGTTGCCTGAACATGTTGAGGATCTAAAGACTATTAGAGAGTTCCGCAATGTGTTTGATGCAGAAGTTTCAAAACTTGAAACTGATAGGCTTCAACTTGGCACAGAGATTGCTGTCACTGGTGATAATTCTTGGCCATTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTTAAAATCGACTTCAGGAGGACTTCTGATATGCATCCAATGGAAATTGTTGAGGCTATTGATAAACTCCAGGAGAGGCTGAGGGTTGTTCCTGGTGATGATTTGTTGAGTGTTGAAGCCCAAAAGAATGCAACTCTCTTTTTCAACATTTTACTTCGCAGCACTTTTGCTAGTAAAAGGGTGTTGGAGGAGTACAGGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGTCACGTTTCCTGCAATCACTAGTAGCGCCTGGTGAAATGATTGGTTGCGTTGCTGCACAATCTATTGGTGAGCCTGCCACTCAGATGACCCTCAATACCTTCCATTATGCTGGTGTGAGTGCAAAAAATGTTACACTAGGTGTTCCCAGGTTGAGGGAAATTATTAATGTTGCTAAGAGGATCAAAACACCATCTCTGTCTGTCTACTTAAAGCCTGAAGCTAATAAAACCAAGGAGAGGGCCAAGAATGTGCAGTGTGCCTTGGAATACACTACCCTACGAAGTGTTACTCAGGCTACAGAAGTATGGTATGATCCAGATCCAATGAGCACATTAATAGAAGAGGATCTTGATTTTGTGAGATCTTACTACGAAATGCCTGATGAAGAGGTCAACCCCGACAAAATTTCTCCGTGGCTGCTTCGCATTGAGTTGAATCGCGAAATGATGGTGGATAAGAAATTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGATGGTGATTTAACTTGTATTTTTAATGATGACAATGCCGAAAAGTTGATCCTTCGAATTCGTATCATGAATGAGGAAACTCCTAAAGGGGAATCACAAGATGAATCTGCTGAAGATGATGTATTCCTGAAGAAGATTGAAAGTAATATGCTTACAGAAATGGCTCTTCGAGGTATCCCAGATATCAACAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGATTCAAGGCAGAGCAAGAGTGGATGTTGGATACAGAGGGTGTTAATCTTTTAGCTGTCCTGTGCCATGATGATGTTGATGCCAGGAGAACAACAAGCAATCATTTGATTGAAATTATTGAAGTTCTTGGGATTGAGGCAGTTCGCCGGTCTTTGCTGGATGAGTTGCGAGTTGTCATATCGTTTGATGGATCTTATGTGAACTACCGCCATCTGGCTATCCTATGTGATACCATGACGTATCGTGGCCACTTGATGGCCATTACTCGTCATGGTATCAATAGAAATGATACCGGCCCAATGATGCGTTGTTCGTTTGAAGAAACTGTGGATATTCTGCTGGATGCTTCTGTTTTTGCAGAAACGGATCATCTGAGGGGTGTAACAGAAAACATTATGCTAGGCCAGTTGGCACCAATTGGGACTGGAGACTGTGCCCTGTATCTCAATGATGAGATGCTAAAGAATGCTATTGAACTCCAGCTGCCTAGTTATATGGATGGCCTTGATTTTGGCATGACCCCATCGCGTTCTCCAGTGTCAGGAACTCCATATCGTGATGGAATGATGTCACCTAATTATTTGCTGAGCCCAAATATGCGAATGTCTCCCATCTCAGATGCTCAGTTTTCTCCCTATGTCGGTGGAATGGCATTCTCTCCTACTTCATCTCCAGGCTACAGTCCATCATCTCCTGGCTACAGTCCATCCTCCCCTGGATACAGTCCCACGTCCCCTGGTTATAGCCCCACGTCCCCAGGTTACAGCCCCACCTCCCCTGGTTATAGTCCCACATCTCCAACCTATAGTCCAAGCTCTCCAGGTTATAGTCCAACCAGTCCGGCATATTCTCCTACAAGTCCGTCTTACTCTCCTACGTCTCCAAGCTACAGCCCTACCTCTCCAAGCTACAGTCCAACCTCTCCAAGCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTCCCCTGCTTATAGCCCGACTTCCCCCGCATACAGCCCCACTTCCCCCGCTTACAGCCCCACTTCACCCTCATACAGCCCAACATCCCCCTCATACAGCCCCACATCCCCCTCCTACAGCCCCACTTCGCCCTCCTACAGCCCCACATCCCCTTCCTACAGCCCCACATCCCCCTCCTACAGCCCAACTTCACCTGCCTACAGCCCAACTTCTCCTGGTTACAGCCCTACTTCCCCAAGTTATAGTCCAACCTCTCCTAGTTATAGTCCTACCTCTCCAAGTTACAATCCTCCTTCTGCAAAATATAGCCCATCGGTAGCCTACTCTCCGAGCAGTCCAAGATTGTCACCAGCTAGCCCATATAGTCCTACCTCTCCAAATTACAGCCCCACATCACCGTCATATTCTCCCACATCACCATCTTATTCTCCCTCTAGCCCCTCATACACTCCCGGCAGCCCATATACCTCTGGAGTGAGCCCCGATTACAGCCCAAGTTCTCCACAGTACAGTCCAAGTGCTGGATACTCACCCAGTCAGCCAGGGTACTCCCCATCATCTACTAGCCAGTACACCCCGCAAACAAGTGAAAAGGATGATAAGGATGACAGGAGCACTCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1779

Amino Acids

198.43

Weight (kDa)

5.84

Isoelectric Point (pI)

64.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	2 - 285	2.1e-95	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	287 - 455	1.1e-71	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	458 - 622	1.2e-52	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	649 - 753	9.3e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	760 - 1350	2.8e-104	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	826 - 1010	1.9e-67	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1095 - 1229	1.2e-53	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	984, 1134, 1278, 1586
AarI	CACCTGC	1 cut(s)	4918
AasI	GACNNNNNNGTC	2 cut(s)	106, 2244
AatII	GACGTC	1 cut(s)	2448
AbsI	CCTCGAGG	1 cut(s)	601
Acc36I	ACCTGC	2 cut(s)	1534, 4918
AccB1I	GGYRCC	3 cut(s)	54, 1363, 4165
AccB7I	CCANNNNNTGG	3 cut(s)	499, 4009, 5237
AccBSI	CCGCTC	1 cut(s)	692
AccI	GTMKAC	1 cut(s)	3216
AccII	CGCG	3 cut(s)	230, 3453, 4282
AccIII	TCCGGA	1 cut(s)	2581
AcoI	YGGCCR	6 cut(s)	21, 524, 633, 2723, 4000, 4011
AcsI	RAATTY	7 cut(s)	219, 1790, 2263, 2431, 3417, 3512, 3573
AcuI	CTGAAG	6 cut(s)	1602, 2762, 2770, 3249, 3648, 3665
AcyI	GRCGYC	1 cut(s)	2445
AdeI	CACNNNGTG	1 cut(s)	3152
AflII	CTTAAG	2 cut(s)	821, 2087
AflIII	ACRYGT	3 cut(s)	228, 1750, 2602
AhlI	ACTAGT	1 cut(s)	3031
AjiI	CACGTC	3 cut(s)	1753, 4485, 4506
Alw21I	GWGCWC	5 cut(s)	325, 1356, 2059, 3344, 5333
AlwNI	CAGNNNCTG	2 cut(s)	1643, 1781
Ama87I	CYCGRG	2 cut(s)	40, 601
Aor13HI	TCCGGA	1 cut(s)	2581
ApaI	GGGCCC	1 cut(s)	1023
ApeKI	GCWGC	9 cut(s)	120, 328, 1781, 1877, 2931, 3065, 3430, 4237, 5172
ApoI	RAATTY	7 cut(s)	219, 1790, 2263, 2431, 3417, 3512, 3573
ArsI	GACNNNNNNTTYG	2 cut(s)	2531, 2563
AseI	ATTAAT	2 cut(s)	3177, 3347
Asp700I	GAANNNNTTC	2 cut(s)	408, 453
AspLEI	GCGC	2 cut(s)	120, 3040
AspS9I	GGNCC	8 cut(s)	163, 774, 1019, 1020, 1772, 2237, 3252, 4049
AsuC2I	CCSGG	4 cut(s)	41, 42, 1977, 5169
AsuII	TTCGAA	1 cut(s)	3571
AsuNHI	GCTAGC	1 cut(s)	5069
AvaI	CYCGRG	2 cut(s)	40, 601
AvaII	GGWCC	3 cut(s)	163, 1772, 2237
BaeGI	GKGCMC	6 cut(s)	41, 57, 1023, 1218, 1368, 4192
BaeI	ACNNNNGTAYC	4 cut(s)	1656, 1689, 2328, 2361
BalI	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
BanI	GGYRCC	3 cut(s)	54, 1363, 4165
BanII	GRGCYC	5 cut(s)	1023, 1356, 2035, 4346, 5198
BauI	CACGAG	2 cut(s)	2034, 2976
BbsI	GAAGAC	1 cut(s)	104
Bbv12I	GWGCWC	5 cut(s)	325, 1356, 2059, 3344, 5333
BbvCI	CCTCAGC	1 cut(s)	2852
BbvI	GCAGC	9 cut(s)	107, 340, 1768, 1889, 2943, 3052, 3417, 4224, 5184
BceAI	ACGGC	3 cut(s)	36, 539, 1565
BciVI	GTATCC	5 cut(s)	1450, 3701, 3778, 4466, 5234
BclI	TGATCA	3 cut(s)	919, 1111, 3742
BcnI	CCSGG	4 cut(s)	41, 42, 1977, 5169
BcuI	ACTAGT	1 cut(s)	3031
BfoI	RGCGCY	1 cut(s)	3041
BfrI	CTTAAG	2 cut(s)	821, 2087
BfuAI	ACCTGC	2 cut(s)	1534, 4918
BfuI	GTATCC	5 cut(s)	1450, 3701, 3778, 4466, 5234
BglI	GCCNNNNNGGC	1 cut(s)	29
BglII	AGATCT	1 cut(s)	3374
BisI	GCNGC	9 cut(s)	121, 329, 1782, 1878, 2932, 3066, 3431, 4238, 5173
BlpI	GCTNAGC	3 cut(s)	69, 324, 4340
BlsI	GCNGC	9 cut(s)	122, 330, 1783, 1879, 2933, 3067, 3432, 4239, 5174
Bme18I	GGWCC	3 cut(s)	163, 1772, 2237
BmeT110I	CYCGRG	2 cut(s)	40, 601
BmgBI	CACGTC	3 cut(s)	1753, 4485, 4506
BmgT120I	GGNCC	8 cut(s)	163, 774, 1019, 1020, 1772, 2237, 3252, 4049
BmiI	GGNNCC	6 cut(s)	56, 1021, 1022, 1365, 1968, 4167
BmrI	ACTGGG	6 cut(s)	1182, 1315, 1572, 1801, 2370, 5244
BmtI	GCTAGC	1 cut(s)	5073
BmuI	ACTGGG	6 cut(s)	1182, 1315, 1572, 1801, 2370, 5244
BpiI	GAAGAC	1 cut(s)	104
Bpu10I	CCTNAGC	1 cut(s)	2852
Bpu1102I	GCTNAGC	3 cut(s)	69, 324, 4340
Bpu14I	TTCGAA	1 cut(s)	3571
BpuMI	CCSGG	4 cut(s)	41, 42, 1977, 5169
BsaAI	YACGTR	1 cut(s)	884
BsaBI	GATNNNNATC	2 cut(s)	2805, 3330
BsaHI	GRCGYC	1 cut(s)	2445
BsaI	GGTCTC	1 cut(s)	568
BsaWI	WCCGGW	1 cut(s)	2581
Bse118I	RCCGGY	2 cut(s)	3900, 4046
Bse3DI	GCAATG	3 cut(s)	2644, 2726, 3489
Bse8I	GATNNNNATC	2 cut(s)	2805, 3330
BseAI	TCCGGA	1 cut(s)	2581
BseJI	GATNNNNATC	2 cut(s)	2805, 3330
BseMI	GCAATG	3 cut(s)	2644, 2726, 3489
BseRI	GAGGAG	6 cut(s)	173, 589, 619, 679, 2389, 2975
BseSI	GKGCMC	6 cut(s)	41, 57, 1023, 1218, 1368, 4192
BseXI	GCAGC	9 cut(s)	107, 340, 1768, 1889, 2943, 3052, 3417, 4224, 5184
BsgI	GTGCAG	2 cut(s)	3051, 3284
Bsh1236I	CGCG	3 cut(s)	230, 3453, 4282
BshNI	GGYRCC	3 cut(s)	54, 1363, 4165
BsiHKAI	GWGCWC	5 cut(s)	325, 1356, 2059, 3344, 5333
BsiHKCI	CYCGRG	2 cut(s)	40, 601
BsiSI	CCGG	7 cut(s)	41, 1976, 2582, 3901, 4047, 4595, 5169
BsiWI	CGTACG	1 cut(s)	884
BsmBI	CGTCTC	1 cut(s)	4638
BsmI	GAATGC	5 cut(s)	1627, 2764, 2905, 4225, 4406
Bso31I	GGTCTC	1 cut(s)	568
BsoBI	CYCGRG	2 cut(s)	40, 601
Bsp119I	TTCGAA	1 cut(s)	3571
Bsp120I	GGGCCC	1 cut(s)	1019
Bsp13I	TCCGGA	1 cut(s)	2581
Bsp1720I	GCTNAGC	3 cut(s)	69, 324, 4340
Bsp19I	CCATGG	1 cut(s)	935
Bsp68I	TCGCGA	1 cut(s)	3453
BspEI	TCCGGA	1 cut(s)	2581
BspFNI	CGCG	3 cut(s)	230, 3453, 4282
BspHI	TCATGA	4 cut(s)	93, 1356, 1482, 3582
BspLI	GGNNCC	6 cut(s)	56, 1021, 1022, 1365, 1968, 4167
BspMAI	CTGCAG	1 cut(s)	2344
BspMI	ACCTGC	2 cut(s)	1534, 4918
BspOI	GCTAGC	1 cut(s)	5073
BspQI	GCTCTTC	1 cut(s)	3687
BspT104I	TTCGAA	1 cut(s)	3571
BspT107I	GGYRCC	3 cut(s)	54, 1363, 4165
BspTI	CTTAAG	2 cut(s)	821, 2087
BspTNI	GGTCTC	1 cut(s)	568
BsrBI	CCGCTC	1 cut(s)	692
BsrDI	GCAATG	3 cut(s)	2644, 2726, 3489
BsrFI	RCCGGY	2 cut(s)	3900, 4046
BssAI	RCCGGY	2 cut(s)	3900, 4046
BssNI	GRCGYC	1 cut(s)	2445
BssSI	CACGAG	2 cut(s)	2034, 2976
BssT1I	CCWWGG	6 cut(s)	770, 935, 1795, 1911, 3243, 3273
Bst2BI	CACGAG	2 cut(s)	2034, 2976
Bst6I	CTCTTC	9 cut(s)	383, 464, 857, 1758, 2057, 2738, 3348, 3393, 3687
BstACI	GRCGYC	1 cut(s)	2445
BstAFI	CTTAAG	2 cut(s)	821, 2087
BstBAI	YACGTR	1 cut(s)	884
BstBI	TTCGAA	1 cut(s)	3571
BstC8I	GCNNGC	7 cut(s)	66, 232, 633, 1786, 2107, 3087, 5071
BstDSI	CCRYGG	3 cut(s)	935, 1547, 3425
BstENI	CCTNNNNNAGG	2 cut(s)	601, 1032
BstFNI	CGCG	3 cut(s)	230, 3453, 4282
BstH2I	RGCGCY	1 cut(s)	3041
BstHHI	GCGC	2 cut(s)	120, 3040
BstMWI	GCNNNNNNNGC	8 cut(s)	29, 61, 70, 126, 135, 334, 1877, 3491
BstNSI	RCATGY	1 cut(s)	2606
BstSLI	GKGCMC	6 cut(s)	41, 57, 1023, 1218, 1368, 4192
BstUI	CGCG	3 cut(s)	230, 3453, 4282
BstV1I	GCAGC	9 cut(s)	107, 340, 1768, 1889, 2943, 3052, 3417, 4224, 5184
BstV2I	GAAGAC	1 cut(s)	104
BstX2I	RGATCY	6 cut(s)	901, 2611, 3331, 3358, 3374, 3944
BstXI	CCANNNNNNTGG	2 cut(s)	1963, 3122
BstYI	RGATCY	6 cut(s)	901, 2611, 3331, 3358, 3374, 3944
BsuI	GTATCC	5 cut(s)	1450, 3701, 3778, 4466, 5234
BtgI	CCRYGG	3 cut(s)	935, 1547, 3425
BtgZI	GCGATG	1 cut(s)	4263
BtrI	CACGTC	3 cut(s)	1753, 4485, 4506
BtsI	GCAGTG	1 cut(s)	3272
BtsIMutI	CAGTG	4 cut(s)	1814, 2706, 3272, 4296
BtuMI	TCGCGA	1 cut(s)	3453
BveI	ACCTGC	2 cut(s)	1534, 4918
Cac8I	GCNNGC	7 cut(s)	66, 232, 633, 1786, 2107, 3087, 5071
CaiI	CAGNNNCTG	2 cut(s)	1643, 1781
CciI	TCATGA	4 cut(s)	93, 1356, 1482, 3582
CfoI	GCGC	2 cut(s)	120, 3040
Cfr10I	RCCGGY	2 cut(s)	3900, 4046
Cfr13I	GGNCC	8 cut(s)	163, 774, 1019, 1020, 1772, 2237, 3252, 4049
Cfr9I	CCCGGG	1 cut(s)	40
CseI	GACGC	3 cut(s)	112, 236, 1133
CsiI	ACCWGGT	1 cut(s)	1033
DraI	TTTAAA	2 cut(s)	297, 2776
DraIII	CACNNNGTG	1 cut(s)	3152
DrdI	GACNNNNNNGTC	2 cut(s)	106, 2244
DseDI	GACNNNNNNGTC	2 cut(s)	106, 2244
EaeI	YGGCCR	6 cut(s)	21, 524, 633, 2723, 4000, 4011
Eam1104I	CTCTTC	9 cut(s)	383, 464, 857, 1758, 2057, 2738, 3348, 3393, 3687
EarI	CTCTTC	9 cut(s)	383, 464, 857, 1758, 2057, 2738, 3348, 3393, 3687
EciI	GGCGGA	1 cut(s)	635
Ecl136II	GAGCTC	1 cut(s)	1354
Eco130I	CCWWGG	6 cut(s)	770, 935, 1795, 1911, 3243, 3273
Eco147I	AGGCCT	1 cut(s)	796
Eco24I	GRGCYC	5 cut(s)	1023, 1356, 2035, 4346, 5198
Eco31I	GGTCTC	1 cut(s)	568
Eco32I	GATATC	1 cut(s)	3700
Eco47I	GGWCC	3 cut(s)	163, 1772, 2237
Eco53kI	GAGCTC	1 cut(s)	1354
Eco57I	CTGAAG	6 cut(s)	1602, 2762, 2770, 3249, 3648, 3665
Eco88I	CYCGRG	2 cut(s)	40, 601
EcoICRI	GAGCTC	1 cut(s)	1354
EcoNI	CCTNNNNNAGG	2 cut(s)	601, 1032
EcoO109I	RGGNCCY	1 cut(s)	1020
EcoRI	GAATTC	2 cut(s)	2263, 3573
EcoRV	GATATC	1 cut(s)	3700
EcoT14I	CCWWGG	6 cut(s)	770, 935, 1795, 1911, 3243, 3273
EcoT22I	ATGCAT	3 cut(s)	1376, 1843, 2808
EcoT38I	GRGCYC	5 cut(s)	1023, 1356, 2035, 4346, 5198
ErhI	CCWWGG	6 cut(s)	770, 935, 1795, 1911, 3243, 3273
Esp3I	CGTCTC	1 cut(s)	4638
FalI	AAGNNNNNCTT	4 cut(s)	3345, 3377, 4603, 4635
FauI	CCCGC	5 cut(s)	697, 1793, 4750, 4771, 5301
FauNDI	CATATG	2 cut(s)	1757, 2188
FbaI	TGATCA	3 cut(s)	919, 1111, 3742
FblI	GTMKAC	1 cut(s)	3216
Fnu4HI	GCNGC	9 cut(s)	121, 329, 1782, 1878, 2932, 3066, 3431, 4238, 5173
FriOI	GRGCYC	5 cut(s)	1023, 1356, 2035, 4346, 5198
Fsp4HI	GCNGC	9 cut(s)	121, 329, 1782, 1878, 2932, 3066, 3431, 4238, 5173
GlaI	GCGC	2 cut(s)	119, 3039
GluI	GCNGC	9 cut(s)	121, 329, 1782, 1878, 2932, 3066, 3431, 4238, 5173
HaeII	RGCGCY	1 cut(s)	3041
HapII	CCGG	7 cut(s)	41, 1976, 2582, 3901, 4047, 4595, 5169
HgaI	GACGC	3 cut(s)	112, 236, 1133
HhaI	GCGC	2 cut(s)	120, 3040
Hin1I	GRCGYC	1 cut(s)	2445
Hin6I	GCGC	2 cut(s)	118, 3038
HinP1I	GCGC	2 cut(s)	118, 3038
HincII	GTYRAC	4 cut(s)	1184, 2737, 3406, 3733
HindII	GTYRAC	4 cut(s)	1184, 2737, 3406, 3733
HpaI	GTTAAC	2 cut(s)	2737, 3733
HpaII	CCGG	7 cut(s)	41, 1976, 2582, 3901, 4047, 4595, 5169
Hpy166II	GTNNAC	8 cut(s)	1184, 2177, 2737, 3217, 3406, 3733, 3955, 5289
Hpy8I	GTNNAC	8 cut(s)	1184, 2177, 2737, 3217, 3406, 3733, 3955, 5289
HpyF10VI	GCNNNNNNNGC	8 cut(s)	29, 61, 70, 126, 135, 334, 1877, 3491
Hsp92I	GRCGYC	1 cut(s)	2445
HspAI	GCGC	2 cut(s)	118, 3038
Kpn2I	TCCGGA	1 cut(s)	2581
Ksp22I	TGATCA	3 cut(s)	919, 1111, 3742
KspAI	GTTAAC	2 cut(s)	2737, 3733
LguI	GCTCTTC	1 cut(s)	3687
LmnI	GCTCC	9 cut(s)	61, 143, 1003, 1123, 1141, 1351, 1534, 1966, 5328
Lsp1109I	GCAGC	9 cut(s)	107, 340, 1768, 1889, 2943, 3052, 3417, 4224, 5184
MabI	ACCWGGT	1 cut(s)	1033
MbiI	CCGCTC	1 cut(s)	692
MfeI	CAATTG	2 cut(s)	1866, 4170
MflI	RGATCY	6 cut(s)	901, 2611, 3331, 3358, 3374, 3944
MlsI	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
MluI	ACGCGT	1 cut(s)	228
MluNI	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
MlyI	GAGTC	3 cut(s)	970, 1643, 3020
MmeI	TCCRAC	9 cut(s)	818, 1329, 1418, 2937, 3762, 4577, 4610, 4694, 4988
Mox20I	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
Mph1103I	ATGCAT	3 cut(s)	1376, 1843, 2808
MroI	TCCGGA	1 cut(s)	2581
MroXI	GAANNNNTTC	2 cut(s)	408, 453
MscI	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
MslI	CAYNNNNRTG	6 cut(s)	1152, 1361, 1493, 2989, 3095, 3825
Msp20I	TGGCCA	4 cut(s)	635, 2725, 4002, 4013
MspA1I	CMGCKG	4 cut(s)	446, 631, 2105, 4237
MspCI	CTTAAG	2 cut(s)	821, 2087
MspI	CCGG	7 cut(s)	41, 1976, 2582, 3901, 4047, 4595, 5169
MunI	CAATTG	2 cut(s)	1866, 4170
Mva1269I	GAATGC	5 cut(s)	1627, 2764, 2905, 4225, 4406
MvnI	CGCG	3 cut(s)	230, 3453, 4282
MwoI	GCNNNNNNNGC	8 cut(s)	29, 61, 70, 126, 135, 334, 1877, 3491
NciI	CCSGG	4 cut(s)	41, 42, 1977, 5169
NcoI	CCATGG	1 cut(s)	935
NdeI	CATATG	2 cut(s)	1757, 2188
NheI	GCTAGC	1 cut(s)	5069
NlaIV	GGNNCC	6 cut(s)	56, 1021, 1022, 1365, 1968, 4167
NmuCI	GTSAC	7 cut(s)	44, 973, 1264, 2704, 3012, 4323, 5061
NruI	TCGCGA	1 cut(s)	3453
NsiI	ATGCAT	3 cut(s)	1376, 1843, 2808
NspI	RCATGY	1 cut(s)	2606
NspV	TTCGAA	1 cut(s)	3571
PaeR7I	CTCGAG	1 cut(s)	601
PagI	TCATGA	4 cut(s)	93, 1356, 1482, 3582
PaqCI	CACCTGC	1 cut(s)	4918
PceI	AGGCCT	1 cut(s)	796
PciI	ACATGT	1 cut(s)	2602
PciSI	GCTCTTC	1 cut(s)	3687
PctI	GAATGC	5 cut(s)	1627, 2764, 2905, 4225, 4406
PdmI	GAANNNNTTC	2 cut(s)	408, 453
Pfl23II	CGTACG	1 cut(s)	884
PflFI	GACNNNGTC	1 cut(s)	1166
PflMI	CCANNNNNTGG	3 cut(s)	499, 4009, 5237
PfoI	TCCNGGA	1 cut(s)	2841
PkrI	GCNGC	9 cut(s)	122, 330, 1783, 1879, 2933, 3067, 3432, 4239, 5174
PleI	GAGTC	3 cut(s)	970, 1642, 3019
PpsI	GAGTC	3 cut(s)	970, 1642, 3019
Ppu21I	YACGTR	1 cut(s)	884
PscI	ACATGT	1 cut(s)	2602
PshBI	ATTAAT	2 cut(s)	3177, 3347
PsiI	TTATAA	4 cut(s)	984, 1134, 1278, 1586
Psp124BI	GAGCTC	1 cut(s)	1356
PspLI	CGTACG	1 cut(s)	884
PspN4I	GGNNCC	6 cut(s)	56, 1021, 1022, 1365, 1968, 4167
PspOMI	GGGCCC	1 cut(s)	1019
PspPI	GGNCC	8 cut(s)	163, 774, 1019, 1020, 1772, 2237, 3252, 4049
PspXI	VCTCGAGB	1 cut(s)	601
PstI	CTGCAG	1 cut(s)	2344
PstNI	CAGNNNCTG	2 cut(s)	1643, 1781
PsuI	RGATCY	6 cut(s)	901, 2611, 3331, 3358, 3374, 3944
PsyI	GACNNNGTC	1 cut(s)	1166
PvuII	CAGCTG	3 cut(s)	631, 2105, 4237
RruI	TCGCGA	1 cut(s)	3453
RseI	CAYNNNNRTG	6 cut(s)	1152, 1361, 1493, 2989, 3095, 3825
SacI	GAGCTC	1 cut(s)	1356
SapI	GCTCTTC	1 cut(s)	3687
SatI	GCNGC	9 cut(s)	121, 329, 1782, 1878, 2932, 3066, 3431, 4238, 5173
Sau96I	GGNCC	8 cut(s)	163, 774, 1019, 1020, 1772, 2237, 3252, 4049
SchI	GAGTC	3 cut(s)	970, 1643, 3020
SexAI	ACCWGGT	1 cut(s)	1033
Sfr274I	CTCGAG	1 cut(s)	601
SfuI	TTCGAA	1 cut(s)	3571
SinI	GGWCC	3 cut(s)	163, 1772, 2237
SlaI	CTCGAG	1 cut(s)	601
SmaI	CCCGGG	1 cut(s)	42
SmiMI	CAYNNNNRTG	6 cut(s)	1152, 1361, 1493, 2989, 3095, 3825
SmlI	CTYRAG	3 cut(s)	601, 821, 2087
SmoI	CTYRAG	3 cut(s)	601, 821, 2087
SpeI	ACTAGT	1 cut(s)	3031
SseBI	AGGCCT	1 cut(s)	796
SspI	AATATT	2 cut(s)	988, 1539
SstI	GAGCTC	1 cut(s)	1356
StuI	AGGCCT	1 cut(s)	796
StyI	CCWWGG	6 cut(s)	770, 935, 1795, 1911, 3243, 3273
TaqI	TCGA	6 cut(s)	602, 1249, 2275, 2781, 3571, 3685
TatI	WGTACW	4 cut(s)	369, 2964, 5223, 5286
TscAI	CASTG	4 cut(s)	1814, 2713, 3272, 4296
TseFI	GTSAC	7 cut(s)	44, 973, 1264, 2704, 3012, 4323, 5061
TseI	GCWGC	9 cut(s)	120, 328, 1781, 1877, 2931, 3065, 3430, 4237, 5172
Tsp45I	GTSAC	7 cut(s)	44, 973, 1264, 2704, 3012, 4323, 5061
TspGWI	ACGGA	4 cut(s)	696, 3414, 4133, 4605
TspMI	CCCGGG	1 cut(s)	40
TspRI	CASTG	4 cut(s)	1814, 2713, 3272, 4296
Tth111I	GACNNNGTC	1 cut(s)	1166
Van91I	CCANNNNNTGG	3 cut(s)	499, 4009, 5237
Vha464I	CTTAAG	2 cut(s)	821, 2087
VpaK11BI	GGWCC	3 cut(s)	163, 1772, 2237
VspI	ATTAAT	2 cut(s)	3177, 3347
XagI	CCTNNNNNAGG	2 cut(s)	601, 1032
XapI	RAATTY	7 cut(s)	219, 1790, 2263, 2431, 3417, 3512, 3573
XceI	RCATGY	1 cut(s)	2606
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1554
XhoI	CTCGAG	1 cut(s)	601
XmaI	CCCGGG	1 cut(s)	40
XmiI	GTMKAC	1 cut(s)	3216
XmnI	GAANNNNTTC	2 cut(s)	408, 453
ZraI	GACGTC	1 cut(s)	2446
Zsp2I	ATGCAT	3 cut(s)	1376, 1843, 2808

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.