Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Reverse (-)

19520407 .. 19529013

Gene Structure 8607 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1237200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5478 bp

ATGGATATCCGGTTTCCCTTCTCGCCAGCGGAGGTGGCGAAGGTGAAATGCGTGCAGTTCGGCATTCTCAGCCCCGACGAAATCAGGCAAATGTCTGTGGTGGAGATTGAGCACAGCGAGACTATGTTGGGAGGCAAACCGAAGACGGCTGGTTTGAGTGATCCTCGGCTCGGTACAATTGACCGGAAGTTGAAATGTGAGACTTGTACAGCCAACATGGCTGAGTGCCCCGGCCATTTTGGGCACCTAGAGCTTGCGAAACCAATGTTTCACATCGGATTCATAAAGACTGTGTTATCTGTAATGCGATGCGTTTGCTTCAATTGCTCGAAAATTCTAGTCGATGAGGACGACCACAAGTTTAAGCAAGCTATGAGAATAAAAAATCCGAAAAACAGGCTGAAAAAGATTTTGGATGCCTGCAAAAACAAATCCAAATGTGAAGGTGGTGATGAAATTGATGTCCAAGGCCAAGATACTGAAGAGCCTGTAAAGAAAAGTCGGGGTGGCTGCGGTGCTCAGCAGCCCAAGCTCTCTATTGAGGGTATGAAAATGAATGCAGAGTACAAAGCCCAAAGGAAGAAAAATGATGACCAGGAACAGCTTCCTGAACCTGTGGAAAGGAAACAGACCCTTACTGCGGAAAGGGTTCTTAGTGTTTTAAAGAGAATAAGTGATGAAGACTCCCAAATATTGGGGTTGAACCCAAAGTATGCTCGGCCTGATTGGATGATTCTACAAGTTCTGCCCATTCCTCCACAACCTGTGAGACCCTCTGTAATGATGGACACCTCATCCAGGAGTGAGGATGATTTGACTCATCAATTGGCGATGATTATTCGTCATAATGACAATCTGAGGCGGCAGGAACGAAACGGTTCTCCTGCTCATATCATTTCAGAGTTTGCACAACTTCTGCAATTCCACATTGCCACTTATTTTGACAATGAGTTGCCAGGACTACCAAGGGCTACGCAGAGATCAGGGAGGCCTATTAAATCAATTTGTAGTAGACTGAAGGCAAAGGAAGGGCGTATAAGAGGCAACTTAATGGGGAAACGTGTTGACTTTTCAGCACGAACAGTGATTACCCCCGATCCAAACATAAACATTGATCAATTAGGAGTGCCATGGAGCATTGCTTTGAATCTCACATACCCTGAGACTGTGACTCCATATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTATGGCCCCCATCCTCCGCCCGCAGCGATCATCATTTGGAGCTTGGATATAAGGCATTGCTATTTCATTCTGATAAGTTTTGATTTGGATCTTTTCTGTTTGAGAGTAAATTTTGAACTTCTACTATTGTGGCTTCAGGTGGAACGTCATTTGAATGACGGAGACTTTATCCTCTTCAATCGACAACCTAGTCTCCATAAAATGTCTATCATGGGCCACAGAATCAAGATCATGCCATACTCAACATTTCGATTGAATTTGTCTGTGACATCGCCATATAATGCTGACTTTGACGGGGATGAAATGAACATGCATGTGCCCCAGTCCTTTGAAACCAGGGCTGAAGTATTGGAGCTCATGATGGTGCCAAAATGCATCGTCTCACCTCAGTCAAATCGACCTGTCATGGGAATTGTGCAGGACACACTTTTAGGTTGTCGTAAGATCACCAAGAGGGACACCTTCATAGAGAAGGATGTTTTCATGAATATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTTCCTGCTCCTGCAATTTTGAAGCCCCGGCCACTTTGGACTGGAAAACAGGTGTTCAATCTTATAATACCAAAACAAATAAATCTCCAAAGAACTTCTGCATGGCACTCGGAATCAGAGAGTGGGAACATAACTCCAGGGGATACTCTTGTTAGAATAGAAAAGGGTGAATTGCTTTCTGGAACTCTGTGCAAGAAGGCCCTTGGGACTTCTACAGGAAGTCTTATTCATGTTATATGGGAAGAGGTTGGTCCAGATGCAGCCCGCAAGTTTCTGGGCCATACCCAATGGCTTGTGAATTACTGGCTGCTGCAGAATGCTTTTAGCATTGGAATTGGAGATACGATTGCTGATGCAGCCACAATGGAGAAAATCAATGAAACAATTTCCAAGGCGAAAAATGAGGTGAAAGATCTTATAAGTAAAGCCCAATCTAAGCAGCTGGAGGCTGAACCTGGACGAACCATGATGGATTCGTTTGAAAACAAAGTGAACCAGGTGTTGAATAGGGCTCGTGATGATGCTGGAAGTAGTGCTCAAAAGAGTTTAGATGAGAGCAACAATCTTAAGGCCATGGTTACAGCAGGGTCCAAAGGGAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAGAACGTTGAGGGTAAGCGAATCCCGTACGGGTTCATAGACCGGACGTTGCCCCACTTTACTAAAGATGATTATGGACCAGAAAGTCGTGGATTTGTAGAGAATTCATACCTGCGTGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGTGGTAGGGAAGGTCTTATAGATACTGCTGTTAAGACATCTGAGACTGGGTACATCCAAAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTGAAATATGATGGCACAGTACGTAATTCTTTGGGTGATGTCATTCAGTTTCTATACGGGGAAGATGGGATGGATGCTGTTTGGATAGAATCGCAAAAGCTGGATTCTTTGAAAATGAAGAAAACAGAATTTGATAAGACTTTTACGTATGAGTTGGATGATGAATATTGGAATCCCGACTACATGTTGCACGAACATGTTGAGGATCTCAAAACGATTAGAGAGTTCCGTAATGTGTTTGATGCAGAAGTTCAGAAACTTGAAACAGATAGATTACAACTTGGAACAGAGATTGCAGTCACAGGTGATAATTCTTGGCCACTGCCTGTTAATTTGAAGAGGCTTATTTGGAATGCACAAAAAACCTTTAAGATTGACTTCCGAAGGACTTCTGATATGCACCCGATGGAAATTGTGGAAGCTATTGATAAGCTCCAGGAGAGACTGAAAGTTGTTCCTGGTGATGACTTGTTGAGTGTCGAAGCACAGAAGAACGCAACATTGTTTTTCAACATTTTACTTCGCAGCACTTTTGCAAGCAAACGGGTGTTGGATGAGTACAGGCTTACTCGTGAAGCATTTGAATGGGTTATTGGGGAGATAGAATCACGCTTCCTGCAATCGCTTGTAGCACCTGGGGAAATGATTGGCTGCGTTGCTGCACAGTCTATTGGAGAACCTGCCACTCAGATGACACTGAATACTTTCCACTACGCAGGTGTGAGTGCAAAGAATGTTACCCTAGGTGTCCCCAGGTTGAGGGAAATTATTAATGTAGCCAAGAGAATCAAAACGCCATCCCTGTCTGTCTACTTAAAGCCTGAAGCTAATAAAACGAAGGAGAGGGCGAAGAATGTGCAGTGTGCTTTGGAATACACCACTCTACGAAGTGTAACTCAAGCTACAGAAGTATGGTACGATCCAGAACCCACAAGCACAATAATTGATGAGGATATAGATTTTGTGAGAGCTTACTATGAAATGCCAGATGAAGAAGTTAATCCTGATAAAATTTCACCTTGGTTGCTTCGCATCGAGTTGAATCGCGAAATGATGGTGGATAAGAAGTTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGATGATGATTTAACTTGTATCTTCAATGATGACAATGCAGAAAAATTGATCCTTCGTATCCGTATCATGAATGCTGAAGCTCCAAAAGGGGAGATGGTTGATGAATCTGCTGAAGATGATGTTTTTCTGAAGAAGATTGAGAGTAACATGCTGACAGAGATGGCTCTTCGTGGTATCCCAGATATTAACAAAGTGTTCATTAAACATGGTAAAGTTAATAAGTTCGATCCAAATGAAGGGTTTAAAGCAGAGCAAGAGTGGATGCTGGATACGGAAGGGGTTAATCTCTTGGCTGTTCTGTGCCATGATGATGTTGATGCTAGGCGCACAACTAGTAATCATTTGATTGAAATTCTGGAAATTCTCGGAATTGAGGCAGTTCGTCGGTCATTGTTGGATGAGTTGAGGGTTGTTATATCATTTGATGGGTCCTATGTTAATTATAGGCATCTCGCTATCCTTTGTGACACGATGACGTACCGTGGGCACTTAATGGCAATAACCCGCCATGGCATTAATAGAAATGACACTGGTCCTATGATGAGGTGCTCCTTTGAAGAGACTGTTGATATTCTTCTTGATGCTGCAGTGTTTGCAGAGACGGATTATTTAAGAGGCGTGACAGAAAATATAATGTTAGGGCAACTTGCACCAATTGGGACGGGAGATTGTGCCCTGTATCTCAATGATGAGATGCTGAAGAACGCCATCGAACTCCAGTTGCCCAGTTACATGGATGGTCTTGATTTTGGTATGACACCATCTCGTTCTCCAGTGTCAGGAACACCATATCACGAGGGCATGATGTCCCCTAATTATTTGCTGAGCCCAAATCTTCGGCTGTCACCAATTTCGGATGCTCAGTTTTCTCCTTATGTTGGAGGAATGGCATTCTCTCCTACTTCCTCTCCAGGCTACAGCCCATCATCCCCTGGATACAGCCCTTCCTCCCCTGGATACAGCCCCACTTCTCCTGGCTACAGTCCCACCTCTCCAGGGTATAGCCCCACTTCTCCTGGGTACAGTCCCACCTCTCCGACATATAGTCCTAGTTCTCCCGGCTATAGTCCCACCAGTCCAGCTTACTCTCCTACGAGTCCATCATACTCGCCAACCTCTCCCAGCTACAGCCCCACCTCTCCAAGCTACAGCCCCACTTCACCAAGCTACAGCCCTACTTCCCCTGCTTACAGCCCCACTTCACCTGCTTACAGCCCCACTTCCCCCGCATATAGCCCCACTTCGCCATCTTACAGTCCCACGTCTCCTTCCTACAGCCCAACTTCGCCATCCTACAGCCCAACTTCGCCTTCCTACAGCCCAACTTCTCCTTCCTACAGTCCAACATCTCCATCCTACAGCCCTACCTCGCCAGCCTACAGCCCAACTTCTCCTGGTTACAGCCCAACCTCGCCTAGTTATAGTCCAACCTCGCCCAGTTACAGCCCTACATCTCCAAGCTATAATCCTCAGTCTGCCAAGTACAGTCCGTCATCAGCTTACTCTCCAAGCAGTCCAAGATTGTCACCATCTAGTCCATACAGTCCTACCTCTCCGAACTACAGCCCAACCTCACCATCATATTCCCCCACATCTCCATCTTACTCTCCCTCAAGCCCCACATACAGTCCCGGCAGCCCATTGACTTCTGGAGTGAGCCTAGACTATAGCTCGAGTTCCCCTCAGTATAGTCCAAGTGCTGGATACTCACCCAGTCAACCCGGTTACTCGCCATCATCTGCTAGCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1826

Amino Acids

203.51

Weight (kDa)

5.61

Isoelectric Point (pI)

64.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	14 - 350	1.4e-114	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	352 - 525	3.8e-63	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	528 - 692	4.6e-52	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	718 - 823	3e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	830 - 1420	3.1e-104	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	896 - 1080	1.1e-67	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1165 - 1299	7.6e-52	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1796, 2150
AarI	CACCTGC	2 cut(s)	3323, 4939
AasI	GACNNNNNNGTC	2 cut(s)	1398, 2454
Acc36I	ACCTGC	4 cut(s)	2490, 3304, 3323, 4939
AccB1I	GGYRCC	2 cut(s)	243, 1573
AccB7I	CCANNNNNTGG	2 cut(s)	694, 5426
AccI	GTMKAC	2 cut(s)	1012, 3426
AccII	CGCG	1 cut(s)	3663
AciI	CCGC	9 cut(s)	29, 513, 641, 862, 1226, 1230, 1996, 4231, 4953
AclI	AACGTT	1 cut(s)	2376
AclWI	GGATC	7 cut(s)	155, 1091, 1305, 2829, 3530, 3769, 3947
AcoI	YGGCCR	3 cut(s)	232, 1760, 2933
AcsI	RAATTY	9 cut(s)	333, 1318, 1465, 2473, 2744, 3627, 3722, 4077, 4086
AcuI	CTGAAG	9 cut(s)	501, 1037, 1328, 1572, 3459, 3822, 3858, 3875, 4445
AflII	CTTAAG	1 cut(s)	2297
AflIII	ACRYGT	3 cut(s)	1060, 2799, 2812
AhdI	GACNNNNNGTC	1 cut(s)	4770
AhlI	ACTAGT	1 cut(s)	4058
AjiI	CACGTC	1 cut(s)	4989
AjuI	GAANNNNNNNTTGG	8 cut(s)	395, 427, 3192, 3224, 5020, 5041, 5052, 5073
Alw21I	GWGCWC	5 cut(s)	114, 520, 1566, 2269, 4277
AlwI	GGATC	7 cut(s)	155, 1091, 1305, 2829, 3530, 3769, 3947
Ama87I	CYCGRG	1 cut(s)	5398
ApoI	RAATTY	9 cut(s)	333, 1318, 1465, 2473, 2744, 3627, 3722, 4077, 4086
ArsI	GACNNNNNNTTYG	2 cut(s)	136, 168
AseI	ATTAAT	2 cut(s)	3387, 4242
Asp700I	GAANNNNTTC	5 cut(s)	603, 648, 877, 3004, 3320
AspA2I	CCTAGG	1 cut(s)	3358
AspLEI	GCGC	1 cut(s)	4053
AspS9I	GGNCC	9 cut(s)	1214, 1423, 1930, 1982, 2008, 2319, 2447, 4155, 4259
AsuC2I	CCSGG	5 cut(s)	231, 1759, 4785, 5358, 5448
AsuNHI	GCTAGC	1 cut(s)	5468
AvaI	CYCGRG	1 cut(s)	5398
AvaII	GGWCC	5 cut(s)	1982, 2319, 2447, 4155, 4259
AvrII	CCTAGG	1 cut(s)	3358
BaeGI	GKGCMC	5 cut(s)	230, 246, 1530, 4215, 4402
BaeI	ACNNNNGTAYC	4 cut(s)	1866, 1899, 2538, 2571
BalI	TGGCCA	1 cut(s)	2935
BanI	GGYRCC	2 cut(s)	243, 1573
BanII	GRGCYC	3 cut(s)	1566, 2245, 4556
BauI	CACGAG	3 cut(s)	2244, 3186, 4520
BbsI	GAAGAC	2 cut(s)	149, 687
Bbv12I	GWGCWC	5 cut(s)	114, 520, 1566, 2269, 4277
BceAI	ACGGC	1 cut(s)	162
BcgI	CGANNNNNNTGC	6 cut(s)	297, 331, 1821, 1855, 3454, 3488
BciVI	GTATCC	7 cut(s)	1867, 3794, 3911, 3988, 4655, 4676, 5423
BclI	TGATCA	1 cut(s)	1114
BcnI	CCSGG	5 cut(s)	231, 1759, 4785, 5358, 5448
BcuI	ACTAGT	1 cut(s)	4058
BfrI	CTTAAG	1 cut(s)	2297
BfuAI	ACCTGC	4 cut(s)	2490, 3304, 3323, 4939
BfuI	GTATCC	7 cut(s)	1867, 3794, 3911, 3988, 4655, 4676, 5423
BglI	GCCNNNNNGGC	1 cut(s)	218
BglII	AGATCT	1 cut(s)	2143
BlnI	CCTAGG	1 cut(s)	3358
BlpI	GCTNAGC	2 cut(s)	519, 4550
Bme18I	GGWCC	5 cut(s)	1982, 2319, 2447, 4155, 4259
BmeRI	GACNNNNNGTC	1 cut(s)	4770
BmeT110I	CYCGRG	1 cut(s)	5398
BmgBI	CACGTC	1 cut(s)	4989
BmgT120I	GGNCC	9 cut(s)	1214, 1423, 1930, 1982, 2008, 2319, 2447, 4155, 4259
BmiI	GGNNCC	5 cut(s)	245, 1216, 1575, 2320, 4156
BmrI	ACTGGG	5 cut(s)	1525, 2580, 4446, 5157, 5433
BmtI	GCTAGC	1 cut(s)	5472
BmuI	ACTGGG	5 cut(s)	1525, 2580, 4446, 5157, 5433
BoxI	GACNNNNGTC	1 cut(s)	4257
BpiI	GAAGAC	2 cut(s)	149, 687
BplI	GAGNNNNNCTC	4 cut(s)	148, 180, 5392, 5424
BpmI	CTGGAG	8 cut(s)	1851, 2195, 3035, 4427, 4482, 4620, 4704, 5397
Bpu1102I	GCTNAGC	2 cut(s)	519, 4550
BpuEI	CTTGAG	2 cut(s)	3498, 5323
BpuMI	CCSGG	5 cut(s)	231, 1759, 4785, 5358, 5448
BsaAI	YACGTR	2 cut(s)	2639, 2763
BsaBI	GATNNNNATC	1 cut(s)	1296
BsaI	GGTCTC	1 cut(s)	763
BsaWI	WCCGGW	3 cut(s)	9, 183, 2412
BsaXI	ACNNNNNCTCC	6 cut(s)	1156, 1186, 1386, 1416, 4618, 4648
Bse3DI	GCAATG	4 cut(s)	927, 1137, 1264, 3699
Bse8I	GATNNNNATC	1 cut(s)	1296
BseJI	GATNNNNATC	1 cut(s)	1296
BseMI	GCAATG	4 cut(s)	927, 1137, 1264, 3699
BseSI	GKGCMC	5 cut(s)	230, 246, 1530, 4215, 4402
BseYI	CCCAGC	1 cut(s)	4847
BsgI	GTGCAG	4 cut(s)	74, 1646, 3261, 3494
Bsh1236I	CGCG	1 cut(s)	3663
BshNI	GGYRCC	2 cut(s)	243, 1573
BsiHKAI	GWGCWC	5 cut(s)	114, 520, 1566, 2269, 4277
BsiHKCI	CYCGRG	1 cut(s)	5398
BsiSI	CCGG	8 cut(s)	10, 184, 231, 1759, 2413, 4785, 5358, 5448
BsiWI	CGTACG	1 cut(s)	2397
BsmBI	CGTCTC	3 cut(s)	1594, 4319, 4995
BsmI	GAATGC	6 cut(s)	63, 562, 2053, 2974, 3802, 4616
Bso31I	GGTCTC	1 cut(s)	763
BsoBI	CYCGRG	1 cut(s)	5398
Bsp1407I	TGTACA	1 cut(s)	206
Bsp1720I	GCTNAGC	2 cut(s)	519, 4550
Bsp19I	CCATGG	3 cut(s)	1130, 2304, 4234
Bsp68I	TCGCGA	1 cut(s)	3663
BspACI	CCGC	9 cut(s)	29, 513, 641, 862, 1226, 1230, 1996, 4231, 4953
BspFNI	CGCG	1 cut(s)	3663
BspHI	TCATGA	3 cut(s)	1566, 1692, 3792
BspLI	GGNNCC	5 cut(s)	245, 1216, 1575, 2320, 4156
BspMAI	CTGCAG	2 cut(s)	2046, 4315
BspMI	ACCTGC	4 cut(s)	2490, 3304, 3323, 4939
BspOI	GCTAGC	1 cut(s)	5472
BspPI	GGATC	7 cut(s)	155, 1091, 1305, 2829, 3530, 3769, 3947
BspQI	GCTCTTC	2 cut(s)	477, 3897
BspT107I	GGYRCC	2 cut(s)	243, 1573
BspTI	CTTAAG	1 cut(s)	2297
BspTNI	GGTCTC	1 cut(s)	763
BsrDI	GCAATG	4 cut(s)	927, 1137, 1264, 3699
BsrGI	TGTACA	1 cut(s)	206
BssSI	CACGAG	3 cut(s)	2244, 3186, 4520
BssT1I	CCWWGG	9 cut(s)	466, 965, 1130, 1933, 2121, 2304, 3358, 3635, 4234
Bst2BI	CACGAG	3 cut(s)	2244, 3186, 4520
Bst6I	CTCTTC	6 cut(s)	477, 1388, 1968, 2948, 3897, 4278
BstAFI	CTTAAG	1 cut(s)	2297
BstAPI	GCANNNNNTGC	1 cut(s)	4319
BstAUI	TGTACA	1 cut(s)	206
BstBAI	YACGTR	2 cut(s)	2639, 2763
BstDSI	CCRYGG	4 cut(s)	1130, 2304, 4207, 4234
BstENI	CCTNNNNNAGG	1 cut(s)	796
BstFNI	CGCG	1 cut(s)	3663
BstHHI	GCGC	1 cut(s)	4053
BstMWI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2087, 3701, 4319
BstNSI	RCATGY	5 cut(s)	1522, 1526, 2803, 2816, 3877
BstPAI	GACNNNNGTC	1 cut(s)	4257
BstSLI	GKGCMC	5 cut(s)	230, 246, 1530, 4215, 4402
BstSNI	TACGTA	2 cut(s)	2639, 2763
BstUI	CGCG	1 cut(s)	3663
BstV2I	GAAGAC	2 cut(s)	149, 687
BstX2I	RGATCY	3 cut(s)	1297, 2143, 2821
BstXI	CCANNNNNNTGG	1 cut(s)	4459
BstYI	RGATCY	3 cut(s)	1297, 2143, 2821
BsuI	GTATCC	7 cut(s)	1867, 3794, 3911, 3988, 4655, 4676, 5423
BtgI	CCRYGG	4 cut(s)	1130, 2304, 4207, 4234
BtgZI	GCGATG	3 cut(s)	322, 845, 1464
BtrI	CACGTC	1 cut(s)	4989
BtsI	GCAGTG	3 cut(s)	2936, 3482, 4320
BtsIMutI	CAGTG	7 cut(s)	1089, 2936, 3311, 3482, 4254, 4320, 4506
BtuMI	TCGCGA	1 cut(s)	3663
BveI	ACCTGC	4 cut(s)	2490, 3304, 3323, 4939
CciI	TCATGA	3 cut(s)	1566, 1692, 3792
CfoI	GCGC	1 cut(s)	4053
Cfr13I	GGNCC	9 cut(s)	1214, 1423, 1930, 1982, 2008, 2319, 2447, 4155, 4259
CsiI	ACCWGGT	1 cut(s)	2226
CspCI	CAANNNNNGTGG	2 cut(s)	922, 957
DraI	TTTAAA	2 cut(s)	663, 3970
DrdI	GACNNNNNNGTC	2 cut(s)	1398, 2454
DriI	GACNNNNNGTC	1 cut(s)	4770
DseDI	GACNNNNNNGTC	2 cut(s)	1398, 2454
EaeI	YGGCCR	3 cut(s)	232, 1760, 2933
Eam1104I	CTCTTC	6 cut(s)	477, 1388, 1968, 2948, 3897, 4278
Eam1105I	GACNNNNNGTC	1 cut(s)	4770
EarI	CTCTTC	6 cut(s)	477, 1388, 1968, 2948, 3897, 4278
EciI	GGCGGA	1 cut(s)	1215
Ecl136II	GAGCTC	1 cut(s)	1564
Eco105I	TACGTA	2 cut(s)	2639, 2763
Eco130I	CCWWGG	9 cut(s)	466, 965, 1130, 1933, 2121, 2304, 3358, 3635, 4234
Eco147I	AGGCCT	1 cut(s)	991
Eco24I	GRGCYC	3 cut(s)	1566, 2245, 4556
Eco31I	GGTCTC	1 cut(s)	763
Eco32I	GATATC	1 cut(s)	7
Eco47I	GGWCC	5 cut(s)	1982, 2319, 2447, 4155, 4259
Eco53kI	GAGCTC	1 cut(s)	1564
Eco57I	CTGAAG	9 cut(s)	501, 1037, 1328, 1572, 3459, 3822, 3858, 3875, 4445
Eco88I	CYCGRG	1 cut(s)	5398
EcoICRI	GAGCTC	1 cut(s)	1564
EcoNI	CCTNNNNNAGG	1 cut(s)	796
EcoO109I	RGGNCCY	2 cut(s)	1930, 4155
EcoRI	GAATTC	1 cut(s)	2473
EcoRV	GATATC	1 cut(s)	7
EcoT14I	CCWWGG	9 cut(s)	466, 965, 1130, 1933, 2121, 2304, 3358, 3635, 4234
EcoT22I	ATGCAT	2 cut(s)	1524, 1586
EcoT38I	GRGCYC	3 cut(s)	1566, 2245, 4556
ErhI	CCWWGG	9 cut(s)	466, 965, 1130, 1933, 2121, 2304, 3358, 3635, 4234
Esp3I	CGTCTC	3 cut(s)	1594, 4319, 4995
FalI	AAGNNNNNCTT	2 cut(s)	2978, 3010
FauI	CCCGC	4 cut(s)	1237, 2003, 4238, 4960
FbaI	TGATCA	1 cut(s)	1114
FblI	GTMKAC	2 cut(s)	1012, 3426
FriOI	GRGCYC	3 cut(s)	1566, 2245, 4556
GlaI	GCGC	1 cut(s)	4052
GsaI	CCCAGC	1 cut(s)	4851
GsuI	CTGGAG	8 cut(s)	1851, 2195, 3035, 4427, 4482, 4620, 4704, 5397
HapII	CCGG	8 cut(s)	10, 184, 231, 1759, 2413, 4785, 5358, 5448
HhaI	GCGC	1 cut(s)	4053
Hin6I	GCGC	1 cut(s)	4051
HinP1I	GCGC	1 cut(s)	4051
HincII	GTYRAC	2 cut(s)	1066, 5444
HindII	GTYRAC	2 cut(s)	1066, 5444
HpaII	CCGG	8 cut(s)	10, 184, 231, 1759, 2413, 4785, 5358, 5448
Hpy166II	GTNNAC	6 cut(s)	1013, 1066, 2224, 2489, 3427, 5444
Hpy8I	GTNNAC	6 cut(s)	1013, 1066, 2224, 2489, 3427, 5444
Hpy99I	CGWCG	2 cut(s)	80, 4113
HpyCH4IV	ACGT	8 cut(s)	1060, 1354, 2376, 2417, 2638, 2762, 4202, 4988
HpyF10VI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2087, 3701, 4319
HpySE526I	ACGT	8 cut(s)	1060, 1354, 2376, 2417, 2638, 2762, 4202, 4988
HspAI	GCGC	1 cut(s)	4051
Ksp22I	TGATCA	1 cut(s)	1114
LguI	GCTCTTC	2 cut(s)	477, 3897
LmnI	GCTCC	8 cut(s)	1134, 1198, 1248, 1561, 1744, 3054, 3811, 4280
MabI	ACCWGGT	1 cut(s)	2226
MaeII	ACGT	8 cut(s)	1060, 1354, 2376, 2417, 2638, 2762, 4202, 4988
MfeI	CAATTG	4 cut(s)	177, 322, 824, 4380
MflI	RGATCY	3 cut(s)	1297, 2143, 2821
MlsI	TGGCCA	1 cut(s)	2935
MluNI	TGGCCA	1 cut(s)	2935
MlyI	GAGTC	4 cut(s)	677, 811, 1165, 4831
MmeI	TCCRAC	7 cut(s)	2751, 3147, 4101, 4585, 4787, 5093, 5177
Mox20I	TGGCCA	1 cut(s)	2935
Mph1103I	ATGCAT	2 cut(s)	1524, 1586
MroXI	GAANNNNTTC	5 cut(s)	603, 648, 877, 3004, 3320
MscI	TGGCCA	1 cut(s)	2935
MslI	CAYNNNNRTG	7 cut(s)	1362, 1523, 1571, 2811, 3199, 3305, 4035
Msp20I	TGGCCA	1 cut(s)	2935
MspA1I	CMGCKG	2 cut(s)	29, 2173
MspCI	CTTAAG	1 cut(s)	2297
MspI	CCGG	8 cut(s)	10, 184, 231, 1759, 2413, 4785, 5358, 5448
MunI	CAATTG	4 cut(s)	177, 322, 824, 4380
Mva1269I	GAATGC	6 cut(s)	63, 562, 2053, 2974, 3802, 4616
MvnI	CGCG	1 cut(s)	3663
MwoI	GCNNNNNNNGC	9 cut(s)	35, 69, 218, 250, 324, 529, 2087, 3701, 4319
NciI	CCSGG	5 cut(s)	231, 1759, 4785, 5358, 5448
NcoI	CCATGG	3 cut(s)	1130, 2304, 4234
NheI	GCTAGC	1 cut(s)	5468
NlaIV	GGNNCC	5 cut(s)	245, 1216, 1575, 2320, 4156
NmeAIII	GCCGAG	2 cut(s)	145, 697
NmuCI	GTSAC	7 cut(s)	1168, 1474, 2914, 4190, 4345, 4569, 5250
NruI	TCGCGA	1 cut(s)	3663
NsiI	ATGCAT	2 cut(s)	1524, 1586
NspI	RCATGY	5 cut(s)	1522, 1526, 2803, 2816, 3877
PaeR7I	CTCGAG	1 cut(s)	5398
PagI	TCATGA	3 cut(s)	1566, 1692, 3792
PaqCI	CACCTGC	2 cut(s)	3323, 4939
PceI	AGGCCT	1 cut(s)	991
PciI	ACATGT	2 cut(s)	2799, 2812
PciSI	GCTCTTC	2 cut(s)	477, 3897
PcsI	WCGNNNNNNNCGW	1 cut(s)	348
PctI	GAATGC	6 cut(s)	63, 562, 2053, 2974, 3802, 4616
PdmI	GAANNNNTTC	5 cut(s)	603, 648, 877, 3004, 3320
Pfl23II	CGTACG	1 cut(s)	2397
PflMI	CCANNNNNTGG	2 cut(s)	694, 5426
PfoI	TCCNGGA	2 cut(s)	797, 3051
PleI	GAGTC	4 cut(s)	677, 811, 1165, 4830
PpsI	GAGTC	4 cut(s)	677, 811, 1165, 4830
Ppu21I	YACGTR	2 cut(s)	2639, 2763
PpuMI	RGGWCCY	1 cut(s)	4155
PscI	ACATGT	2 cut(s)	2799, 2812
PshAI	GACNNNNGTC	1 cut(s)	4257
PshBI	ATTAAT	2 cut(s)	3387, 4242
PsiI	TTATAA	2 cut(s)	1796, 2150
Psp124BI	GAGCTC	1 cut(s)	1566
Psp1406I	AACGTT	1 cut(s)	2376
Psp5II	RGGWCCY	1 cut(s)	4155
PspFI	CCCAGC	1 cut(s)	4847
PspLI	CGTACG	1 cut(s)	2397
PspN4I	GGNNCC	5 cut(s)	245, 1216, 1575, 2320, 4156
PspPI	GGNCC	9 cut(s)	1214, 1423, 1930, 1982, 2008, 2319, 2447, 4155, 4259
PspPPI	RGGWCCY	1 cut(s)	4155
PspXI	VCTCGAGB	1 cut(s)	5398
PsrI	GAACNNNNNNTAC	2 cut(s)	1072, 1104
PstI	CTGCAG	2 cut(s)	2046, 4315
PsuI	RGATCY	3 cut(s)	1297, 2143, 2821
PvuII	CAGCTG	1 cut(s)	2173
RruI	TCGCGA	1 cut(s)	3663
RseI	CAYNNNNRTG	7 cut(s)	1362, 1523, 1571, 2811, 3199, 3305, 4035
SacI	GAGCTC	1 cut(s)	1566
SapI	GCTCTTC	2 cut(s)	477, 3897
Sau96I	GGNCC	9 cut(s)	1214, 1423, 1930, 1982, 2008, 2319, 2447, 4155, 4259
SchI	GAGTC	4 cut(s)	677, 811, 1165, 4831
SexAI	ACCWGGT	1 cut(s)	2226
Sfr274I	CTCGAG	1 cut(s)	5398
SinI	GGWCC	5 cut(s)	1982, 2319, 2447, 4155, 4259
SlaI	CTCGAG	1 cut(s)	5398
SmiMI	CAYNNNNRTG	7 cut(s)	1362, 1523, 1571, 2811, 3199, 3305, 4035
SmlI	CTYRAG	4 cut(s)	2297, 3513, 5338, 5398
SmoI	CTYRAG	4 cut(s)	2297, 3513, 5338, 5398
SnaBI	TACGTA	2 cut(s)	2639, 2763
SpeI	ACTAGT	1 cut(s)	4058
SseBI	AGGCCT	1 cut(s)	991
SsiI	CCGC	9 cut(s)	29, 513, 641, 862, 1226, 1230, 1996, 4231, 4953
SspI	AATATT	3 cut(s)	693, 1183, 2783
SstI	GAGCTC	1 cut(s)	1566
StuI	AGGCCT	1 cut(s)	991
StyI	CCWWGG	9 cut(s)	466, 965, 1130, 1933, 2121, 2304, 3358, 3635, 4234
TaiI	ACGT	8 cut(s)	1063, 1357, 2379, 2420, 2641, 2765, 4205, 4991
TaqII	GACCGA	1 cut(s)	4101
TatI	WGTACW	4 cut(s)	206, 564, 3174, 5208
TauI	GCSGC	1 cut(s)	865
TscAI	CASTG	7 cut(s)	1089, 2943, 3318, 3482, 4261, 4320, 4506
TseFI	GTSAC	7 cut(s)	1168, 1474, 2914, 4190, 4345, 4569, 5250
Tsp45I	GTSAC	7 cut(s)	1168, 1474, 2914, 4190, 4345, 4569, 5250
TspGWI	ACGGA	6 cut(s)	1383, 2834, 3776, 4013, 4343, 5205
TspRI	CASTG	7 cut(s)	1089, 2943, 3318, 3482, 4261, 4320, 4506
Van91I	CCANNNNNTGG	2 cut(s)	694, 5426
Vha464I	CTTAAG	1 cut(s)	2297
VpaK11BI	GGWCC	5 cut(s)	1982, 2319, 2447, 4155, 4259
VspI	ATTAAT	2 cut(s)	3387, 4242
XagI	CCTNNNNNAGG	1 cut(s)	796
XapI	RAATTY	9 cut(s)	333, 1318, 1465, 2473, 2744, 3627, 3722, 4077, 4086
XceI	RCATGY	5 cut(s)	1522, 1526, 2803, 2816, 3877
XhoI	CTCGAG	1 cut(s)	5398
XmaJI	CCTAGG	1 cut(s)	3358
XmiI	GTMKAC	2 cut(s)	1012, 3426
XmnI	GAANNNNTTC	5 cut(s)	603, 648, 877, 3004, 3320
Zsp2I	ATGCAT	2 cut(s)	1524, 1586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.