Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002476

Physical Location & Seq

Genomic Coordinates Reverse (-)

10324578 .. 10332826

Gene Structure 8249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG123900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5367 bp

ATGGATATTCGCTTCCCTTTCTCGCCGTGCGAGGTCGCCAAGGTCCGCCGCGTCCAGTTCGGCATACTGAGCCCAGACGAAATCAGGCAAATGTCTGTGGTGCAGATTGAGCACAGTGAGACAATGCTGGGGGGAAAGCCGAAGACGGCGGGGTTGAGTGACCCTCGGCTGGGGACAATTGACCGGAAGATGAAGTGTGAGACTTGCACGGCCAACATGGCTGAGTGCCCGGGTCATTTTGGGCACCTGGAGCTGGCCAAGCCCATGTTTCATATTGGGTTCATGAAGACTGTGTTGTCTATAATGCGCTGCGTCTGCTTCAACTGCTCCAAAATCCTAGTTGATGAGGAGGAGCACAAGTTTAAGACAGCTTTGAGAATCAAGAACCCAAAAAATAGGCTAAAGAAAATTTTGGACGCGTGCAAAAACAAAACCAAGTGTGAAGGCGGTGATGAGATTGATGTGCAAGAATCGGACGAACCTTTAAAGAAAAGTCGGGGTGGCTGTGGTGCTCAGCAGCCGAAGCTCTCTATAGATGGTATGAAGATGGTTGCAGAGTACAAAGCCCAAAGAAAGAAGAGTGATGACCAGGAACAGCTTCCTGAACCTGTGGAAAGGAAGCAGACCCTTACCGCTGAAAGGGTTCTTAGTGTGTTGAAGAGGATAAGTGATGAAGATTGCCAATTATTGGGATTGAACCCCAAGTATGCACGTCCAGATTGGATGATTTTACAAGTTCTTCCTATTCCTCCACAACCTGTGAGACCCTCTGTGATGATGGACACCTCCTCGAGGAGTGAGGATGATTTAACACATCAGCTGGCCATGATTATCCGCCACAACGAGAATCTGAGGAGACAGGAAAGAAACGGATCACCCGCTCATATCATCTCAGAGTTTGCACAACTCTTGCAATTCCATATAGCTACATATTTTGATAATGAGTTGCCTGGACTACCAAGGGCCACACAGAGATCAGGGAGGCCTATAAAATCAATTTGTAGTAGGCTTAAGGCGAAGGAAGGTCGGATTAGAGGAAACTTAATGGGGAAGAGGGTTGATTTCTCAGCACGTACGGTTATTACACCAGATCCGAACATTAACATTGATCAATTAGGAGTACCATGGAGTATTGCGCTGAATCTCACATACCCTGAAACTGTGACTCCTTATAATATTGAAAGGTTGAAGGAGCTTGTTGAGTACGGGCCCCATCCTCCACCAGGTAAAACTGGTGCAAAGTATATAATAAGAGATGATGGGCAAAGGCTTGATCTACGGTACTTGAAGAAAAGTAGTGATCATCATTTGGAGCTTGGTTATAAGGTGGAGCGTCATTTGAATGATGGAGACTTTGTCCTCTTTAATCGTCAACCCAGTCTCCATAAAATGTCTATTATGGGGCACAGAATCAAAATCATGCCTTACTCAACTTTTCGATTAAATCTATCTGTCACTTCGCCTTATAATGCTGATTTTGATGGGGATGAAATGAATATGCACGTTCCCCAGTCATTTGAAACTAGGGCAGAAGTGTTGGAGCTCATGATGGTGCCCAAATGCATTGTGTCCCCTCAATCAAATCGTCCTGTGATGGGAATTGTCCAAGATACCCTGTTGGGTTGTCGTAAGATCACCAAGAGGGATACCTTCATAGAGAAGGATGTTTTCATGAACATCTTGATGTGGTGGGAGGATTTTGATGGAAAAGTACCTGCTCCTGCAATATTGAAACCACGGCCTCTTTGGACTGGGAAACAGGTGTTTAATCTTATAATACCAAAGCAAATAAATCTCCAGAGAACTTCAGCGTGGCACTCTGAATCAGAAACTGGTAACATTACCCCTGGTGATACTCACGTAAGAATAGAAAAAGGGGAGTTACTTTCCGGAACTCTGTGCAAAAAGGCACTTGGGACATCTACTGGAAGTCTTATACACGTCATATGGGAAGAGGTTGGTCCAGATGCTGCCCGCAAATTCCTTGGTCATACCCAGTGGCTTGTGAATTACTGGCTATTGCAGAATGCATTTAGCATTGGAATTGGAGATACAATTGCTGATGCAGCAACGATGGAAAAGATCAATGAAACTATTTCCAAGGCAAAAAATGAAGTGAAAGAACTTATCAGTAAAGCCCAATCCAAGAGCCTGGAGCCTGAACCGGGGCGAACTATGATGGATTCCTTTGAAAACAAAGTGAATCAGGTCTTAAATAGGGCTCGTGATGATGCTGGAAGTAGTGCTCAGAAGAGTTTGTCTGAGAGTAACAATCTTAAGGCTATGGTTACAGCTGGCTCTAAGGGAAGTTTTATCAACATATCACAGATGACTGCTTGTGTGGGACAGCAGAATGTTGAGGGTAAACGAATCCCATATGGGTTCATAGACCGTACTTTGCCCCACTTCACTAAGGATGACTATGGACCAGAAAGTCGTGGCTTTGTAGAGAATTCATACCTTCGAGGACTAACTCCACAGGAGTTCTTTTTTCATGCTATGGGAGGTAGGGAAGGTCTTATAGATACTGCAGTTAAGACATCCGAGACTGGGTATATCCAGAGGAGACTGGTGAAGGCTATGGAGGATATTATGGTCAAGTATGATGGGACTGTGAGAAATTCGTTGGGGGACGTCATACAGTTTCTCTATGGAGAAGATGGAATGGATGCTGTATGGATAGAATCACAAAAGCTGGATTCTCTTAAAATGAAGAAGAAAGAGTTTGACAAGACTTTCGCTTATGTGTTTGATGATGAAAATTGGAATCCGGACTATATGTTGCCTGAACATGTTGAGGATCTAAAGACTATTAGAGAGTTCCGCAATGTGTTTGATGCAGAAGTTTCAAAACTTGAAACTGATAGGCTTCAACTTGGCACAGAGATTGCTGTCACTGGTGATAATTCTTGGCCATTGCCTGTTAACCTGAAGAGGCTCATTTGGAATGCACAAAAAACTTTTAAAATCGACTTCAGGAGGACTTCTGATATGCATCCAATGGAAATTGTTGAGGCTATTGATAAACTCCAGGAGAGGCTAAGGGTTGTTCCTGGTGATGATTTGTTGAGTGTTGAAGCCCAAAAGAATGCAACTCTCTTTTTCAACATTTTGCTTCGTAGCACTTTCGCTAGTAAAAGGGTGTTGGAGGAGTACAGGCTTACTCGTGAAGCATTTGAGTGGGTTATTGGGGAGATAGAGTCACGTTTCCTGCAATCACTGGTAGCGCCTGGTGAAATGATTGGTTGTGTTGCTGCACAATCTATTGGTGAACCTGCCACTCAGATGACCCTCAATACCTTCCATTATGCTGGTGTGAGTGCAAAAAATGTTACACTAGGTGTTCCCAGGTTGAGGGAAATTATTAATGTTGCCAAAAGGATCAAAACACCATCTCTGTCTGTCTACTTAAAACCTGAAGCTAATAAAACCAAGGAGAGGGCCAAGAATGTGCAGTGTGCCTTGGAATACACTACCCTACGAAGTGTTACTCAGGCTACAGAAGTATGGTATGATCCAGATCCAATGAGCACATTAATAGAAGAGGATCTTGATTTTGTGAGATCTTACTACGAAATGCCAGATGAAGAGGTCAACCCCGACAAAATTTCTCCGTGGCTGCTTCGTATTGAGTTGAATCGCGAAATGATGGTGGATAAGAAATTGAGCATGGCTGACATTGCTGAGAAGATTAACCTTGAATTTGATGGTGATTTAACTTGTATTTTTAATGATGACAATGCCGAAAAGTTGATCCTTCGAATTCGTATCATGAATGAGGAAACTCCTAAAGGGGAATCACAAGATGAATCTGCTGAAGATGATGTATTCCTGAAGAAGATTGAAAGTAATATGCTTACAGAAATGGCTCTTCGAGGTATCCCAGATATCAACAAAGTGTTCATAAAGCATGGTAAAGTTAACAAGTTTGATCAGAATGAAGGATTCAAGGCAGAGCAAGAGTGGATGTTGGATACAGAGGGTGTTAATCTTTTAGCCGTCCTGTGCCATGATGATGTTGATGCCAGGAGAACGACAAGCAATCATTTGATTGAAATTATTGAAGTTCTTGGGATTGAGGCAGTTCGCCGGTCTTTGCTGGATGAGTTGCGAGTTGTCATATCGTTTGATGGATCTTATGTGAACTACCGCCATCTGGCTATCCTATGTGATACCATGACGTATCGTGGCCACTTGATGGCCATTACTCGTCATGGTATAAATAGAAATGATACCGGCCCAATGATGCGTTGTTCGTTCGAAGAAACTGTGGATATTCTGCTGGATGCTTCTGTTTTTGCAGAAACGGATCATCTGAGGGGTGTAACAGAAAACATTATGCTAGGCCAGTTGGCACCAATTGGGACTGGAGACTGTGCCCTGTATCTCAATGATGAGATGCTAAAGAATGCTATTGAACTCCAGCTGCCTAGTTATATGGATGGCCTTGATTTTGGCATGACCCCATCGCGTTCTCCAGTGTCAGGAACTCCATATCGTGATGGAATGATGTCACCTAACTATTTGCTGAGCCCAAATATGCGAATGTCTCCCATCTCAGATGCTCAGTTTTCTCCCTATGTCGGTGGAATGGCATTCTCTCCTACTTCATCTCCAGGCTACAGCCCATCATCCCCTGGCTACAGTCCATCATCCCCAGGTTACAGCCCCACGTCCCCTGGTTATAGCCCCACATCCCCAGGTTACAGCCCCACCTCCCCTGGTTATAGTCCCACTTCTCCAACCTATAGTCCAAGCTCTCCAGGTTATAGTCCAACCAGTCCGGCATATTCTCCTACAAGTCCGTCTTACTCTCCTACATCTCCAAGCTACAGCCCTACCTCTCCAAGCTACAGTCCAACCTCTCCAAGCTACAGCCCGACCTCTCCAAGCTACAGCCCGACTTCCCCCGCTTACAGCCCGACTTCCCCCGCATACAGCCCGACTTCCCCCGCTTACAGCCCCACTTCCCCATCATACAGCCCAACATCTCCCTCATACAGCCCCACTTCGCCCTCCTACAGCCCCACTTCGCCCTCCTACAGCCCCACATCCCCCTCCTACAGCCCAACATCCCCCTCCTACAGCCCAACTTCACCTGCCTACAGCCCAACTTCTCCTGGTTACAGCCCTACTTCCCCAAGTTATAGTCCAACCTCTCCTAGTTATAGCCCTACCTCTCCAAGTTACAATCCTCCTTCTGCAAAATATAGCCCATCGGTAGCTTACTCTCCGAGCAGTCCAAGATTGTCACCAGCTAGCCCATATAGTCCTACCTCTCCAAATTACAGCCCCACATCACCGTCATATTCTCCCACATCACCATCTTATTCTCCCTCTAGCCCCTCATACACTCCCGGCAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003899 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005665 GO:0005737 GO:0005773 GO:0005911 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0016070 GO:0016591 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097159 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5 RNA_pol_Rpb1_6 RNA_pol_Rpb1_7 RNA_pol_Rpb1_R

Protein Analysis

1788

Amino Acids

199.77

Weight (kDa)

6.03

Isoelectric Point (pI)

63.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	14 - 348	1.7e-115	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	350 - 518	1.1e-71	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	521 - 685	1.2e-52	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	712 - 816	9.4e-38	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	823 - 1413	2.9e-104	RNA polymerase Rpb1, domain 5
RNA_pol_Rpb1_6	PF04992	889 - 1073	1.9e-67	RNA polymerase Rpb1, domain 6
RNA_pol_Rpb1_7	PF04990	1158 - 1292	1.2e-53	RNA polymerase Rpb1, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35800 AT4G35800
fragaria_vesca	FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070 FvH4_1g11070
malus_domestica	MD02G1123200.v1.1 MD02G1123400.v1.1 MD02G1125500.v1.1 MD02G1125800.v1.1 MD15G1237200.v1.1
prunus_persica	Prupe.7G175200_v2.0.a1
pyrus_communis	pycom02g09610 pycom15g21110
rosa_chinensis	RchiOBHm_Chr2g0098321 RchiOBHm_Chr2g0098331
rosa_laevigata	RLG00000016790 RLG00000016791
rosa_multiflora	Rmu_co8358581.1_g000001 Rmu_sc0003434.1_g000008 Rmu_sc0023772.1_g000002 Rmu_sc0042863.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144060 Rroxscaffold_2G00144070
rosa_rugosa	Rorug02G0072000 Rorug02G0072000 Rorug02G0072100
rosa_samantha	Rh2AG119100 Rh2AG119200 Rh2BG122300 Rh2BG122400 Rh2CG123900 Rh2CG124000 Rh2DG123900 Rh2DG124000
rosa_wichuraiana	Rw2G009330 Rw2G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1173, 1323, 1467, 1775
AarI	CACCTGC	1 cut(s)	5107
AasI	GACNNNNNNGTC	2 cut(s)	295, 2433
AatII	GACGTC	1 cut(s)	2637
AbsI	CCTCGAGG	1 cut(s)	790
Acc36I	ACCTGC	4 cut(s)	1723, 3283, 5107, 5352
AccB1I	GGYRCC	3 cut(s)	243, 1552, 4354
AccB7I	CCANNNNNTGG	2 cut(s)	688, 4198
AccBSI	CCGCTC	1 cut(s)	881
AccI	GTMKAC	1 cut(s)	3405
AccII	CGCG	4 cut(s)	51, 419, 3642, 4471
AccIII	TCCGGA	2 cut(s)	1889, 2770
AcoI	YGGCCR	6 cut(s)	210, 255, 822, 2912, 4189, 4200
AcsI	RAATTY	7 cut(s)	408, 1979, 2452, 2620, 3606, 3701, 3762
AcuI	CTGAAG	6 cut(s)	1791, 2951, 2959, 3438, 3837, 3854
AcyI	GRCGYC	1 cut(s)	2634
AdeI	CACNNNGTG	1 cut(s)	3341
AfaI	GTAC	8 cut(s)	560, 1075, 1122, 1205, 1283, 1713, 2395, 3155
AflII	CTTAAG	2 cut(s)	1010, 2276
AflIII	ACRYGT	3 cut(s)	417, 1939, 2791
AjiI	CACGTC	3 cut(s)	713, 1942, 4674
Alw21I	GWGCWC	6 cut(s)	114, 357, 514, 1545, 2248, 3533
AlwNI	CAGNNNCTG	2 cut(s)	1832, 1970
Ama87I	CYCGRG	2 cut(s)	229, 790
Aor13HI	TCCGGA	2 cut(s)	1889, 2770
ApaI	GGGCCC	1 cut(s)	1212
ApeKI	GCWGC	7 cut(s)	309, 517, 1970, 2066, 3254, 3619, 4426
ApoI	RAATTY	7 cut(s)	408, 1979, 2452, 2620, 3606, 3701, 3762
ArsI	GACNNNNNNTTYG	2 cut(s)	2720, 2752
AseI	ATTAAT	2 cut(s)	3366, 3536
Asp700I	GAANNNNTTC	2 cut(s)	597, 642
AspLEI	GCGC	3 cut(s)	309, 1138, 3229
AspS9I	GGNCC	8 cut(s)	43, 963, 1208, 1209, 1961, 2426, 3441, 4238
AsuC2I	CCSGG	4 cut(s)	230, 231, 2166, 5358
AsuII	TTCGAA	2 cut(s)	3760, 4260
AsuNHI	GCTAGC	1 cut(s)	5258
AvaI	CYCGRG	2 cut(s)	229, 790
AvaII	GGWCC	3 cut(s)	43, 1961, 2426
BaeGI	GKGCMC	6 cut(s)	230, 246, 1212, 1407, 1557, 4381
BaeI	ACNNNNGTAYC	4 cut(s)	1845, 1878, 2517, 2550
BalI	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
BanI	GGYRCC	3 cut(s)	243, 1552, 4354
BanII	GRGCYC	5 cut(s)	74, 1212, 1545, 2224, 4535
BauI	CACGAG	2 cut(s)	2223, 3165
BbsI	GAAGAC	2 cut(s)	149, 293
Bbv12I	GWGCWC	6 cut(s)	114, 357, 514, 1545, 2248, 3533
BbvI	GCAGC	7 cut(s)	296, 529, 1957, 2078, 3241, 3606, 4413
BceAI	ACGGC	5 cut(s)	10, 162, 225, 1754, 3983
BciVI	GTATCC	3 cut(s)	1639, 3890, 3967
BclI	TGATCA	3 cut(s)	1108, 1300, 3931
BcnI	CCSGG	4 cut(s)	230, 231, 2166, 5358
BfaI	CTAG	9 cut(s)	338, 1524, 3132, 3338, 4343, 4431, 5163, 5259, 5340
BfoI	RGCGCY	1 cut(s)	3230
BfrI	CTTAAG	2 cut(s)	1010, 2276
BfuAI	ACCTGC	4 cut(s)	1723, 3283, 5107, 5352
BfuI	GTATCC	3 cut(s)	1639, 3890, 3967
BglI	GCCNNNNNGGC	1 cut(s)	218
BglII	AGATCT	1 cut(s)	3563
BisI	GCNGC	8 cut(s)	49, 310, 518, 1971, 2067, 3255, 3620, 4427
BlpI	GCTNAGC	2 cut(s)	513, 4529
BlsI	GCNGC	8 cut(s)	50, 311, 519, 1972, 2068, 3256, 3621, 4428
Bme18I	GGWCC	3 cut(s)	43, 1961, 2426
BmeT110I	CYCGRG	2 cut(s)	229, 790
BmgBI	CACGTC	3 cut(s)	713, 1942, 4674
BmgT120I	GGNCC	8 cut(s)	43, 963, 1208, 1209, 1961, 2426, 3441, 4238
BmiI	GGNNCC	6 cut(s)	245, 1210, 1211, 1554, 2157, 4356
BmrI	ACTGGG	5 cut(s)	1371, 1504, 1761, 1990, 2559
BmtI	GCTAGC	1 cut(s)	5262
BmuI	ACTGGG	5 cut(s)	1371, 1504, 1761, 1990, 2559
BpiI	GAAGAC	2 cut(s)	149, 293
BplI	GAGNNNNNCTC	2 cut(s)	148, 180
BpmI	CTGGAG	9 cut(s)	269, 1781, 2174, 3014, 4389, 4406, 4461, 4599, 4746
Bpu10I	CCTNAGC	1 cut(s)	3041
Bpu1102I	GCTNAGC	2 cut(s)	513, 4529
Bpu14I	TTCGAA	2 cut(s)	3760, 4260
BpuMI	CCSGG	4 cut(s)	230, 231, 2166, 5358
BsaAI	YACGTR	2 cut(s)	1073, 1861
BsaBI	GATNNNNATC	2 cut(s)	2994, 3519
BsaHI	GRCGYC	1 cut(s)	2634
BsaI	GGTCTC	1 cut(s)	757
BsaWI	WCCGGW	3 cut(s)	183, 1889, 2770
Bse118I	RCCGGY	2 cut(s)	4089, 4235
Bse3DI	GCAATG	3 cut(s)	2833, 2915, 3678
Bse8I	GATNNNNATC	2 cut(s)	2994, 3519
BseAI	TCCGGA	2 cut(s)	1889, 2770
BseJI	GATNNNNATC	2 cut(s)	2994, 3519
BseMI	GCAATG	3 cut(s)	2833, 2915, 3678
BseRI	GAGGAG	7 cut(s)	362, 365, 778, 808, 868, 2578, 3164
BseSI	GKGCMC	6 cut(s)	230, 246, 1212, 1407, 1557, 4381
BseXI	GCAGC	7 cut(s)	296, 529, 1957, 2078, 3241, 3606, 4413
BseYI	CCCAGC	2 cut(s)	127, 169
BsgI	GTGCAG	3 cut(s)	122, 3240, 3473
Bsh1236I	CGCG	4 cut(s)	51, 419, 3642, 4471
BshNI	GGYRCC	3 cut(s)	243, 1552, 4354
BsiHKAI	GWGCWC	6 cut(s)	114, 357, 514, 1545, 2248, 3533
BsiHKCI	CYCGRG	2 cut(s)	229, 790
BsiSI	CCGG	9 cut(s)	184, 230, 1890, 2165, 2771, 4090, 4236, 4784, 5358
BsiWI	CGTACG	1 cut(s)	1073
BslFI	GGGAC	9 cut(s)	187, 1555, 1930, 2358, 2623, 2645, 4378, 4660, 4716
BsmFI	GGGAC	9 cut(s)	187, 1555, 1930, 2358, 2623, 2645, 4378, 4660, 4716
BsmI	GAATGC	5 cut(s)	2032, 2953, 3094, 4414, 4595
Bso31I	GGTCTC	1 cut(s)	757
BsoBI	CYCGRG	2 cut(s)	229, 790
Bsp119I	TTCGAA	2 cut(s)	3760, 4260
Bsp120I	GGGCCC	1 cut(s)	1208
Bsp13I	TCCGGA	2 cut(s)	1889, 2770
Bsp1720I	GCTNAGC	2 cut(s)	513, 4529
Bsp19I	CCATGG	1 cut(s)	1124
Bsp68I	TCGCGA	1 cut(s)	3642
BspEI	TCCGGA	2 cut(s)	1889, 2770
BspFNI	CGCG	4 cut(s)	51, 419, 3642, 4471
BspHI	TCATGA	4 cut(s)	282, 1545, 1671, 3771
BspLI	GGNNCC	6 cut(s)	245, 1210, 1211, 1554, 2157, 4356
BspMAI	CTGCAG	1 cut(s)	2533
BspMI	ACCTGC	4 cut(s)	1723, 3283, 5107, 5352
BspOI	GCTAGC	1 cut(s)	5262
BspQI	GCTCTTC	1 cut(s)	3876
BspT104I	TTCGAA	2 cut(s)	3760, 4260
BspT107I	GGYRCC	3 cut(s)	243, 1552, 4354
BspTI	CTTAAG	2 cut(s)	1010, 2276
BspTNI	GGTCTC	1 cut(s)	757
BsrBI	CCGCTC	1 cut(s)	881
BsrDI	GCAATG	3 cut(s)	2833, 2915, 3678
BsrFI	RCCGGY	2 cut(s)	4089, 4235
BssAI	RCCGGY	2 cut(s)	4089, 4235
BssNI	GRCGYC	1 cut(s)	2634
BssSI	CACGAG	2 cut(s)	2223, 3165
BssT1I	CCWWGG	7 cut(s)	39, 959, 1124, 1984, 2100, 3432, 3462
Bst2BI	CACGAG	2 cut(s)	2223, 3165
Bst6I	CTCTTC	9 cut(s)	572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
BstACI	GRCGYC	1 cut(s)	2634
BstAFI	CTTAAG	2 cut(s)	1010, 2276
BstBAI	YACGTR	2 cut(s)	1073, 1861
BstBI	TTCGAA	2 cut(s)	3760, 4260
BstC8I	GCNNGC	6 cut(s)	255, 421, 822, 1975, 2296, 5260
BstDSI	CCRYGG	3 cut(s)	1124, 1736, 3614
BstENI	CCTNNNNNAGG	2 cut(s)	790, 1221
BstFNI	CGCG	4 cut(s)	51, 419, 3642, 4471
BstH2I	RGCGCY	1 cut(s)	3230
BstHHI	GCGC	3 cut(s)	309, 1138, 3229
BstNSI	RCATGY	1 cut(s)	2795
BstSLI	GKGCMC	6 cut(s)	230, 246, 1212, 1407, 1557, 4381
BstUI	CGCG	4 cut(s)	51, 419, 3642, 4471
BstV1I	GCAGC	7 cut(s)	296, 529, 1957, 2078, 3241, 3606, 4413
BstV2I	GAAGAC	2 cut(s)	149, 293
BstX2I	RGATCY	6 cut(s)	1090, 2800, 3520, 3547, 3563, 4133
BstXI	CCANNNNNNTGG	2 cut(s)	2152, 3311
BstYI	RGATCY	6 cut(s)	1090, 2800, 3520, 3547, 3563, 4133
BsuI	GTATCC	3 cut(s)	1639, 3890, 3967
BtgI	CCRYGG	3 cut(s)	1124, 1736, 3614
BtgZI	GCGATG	1 cut(s)	4452
BtrI	CACGTC	3 cut(s)	713, 1942, 4674
BtsI	GCAGTG	1 cut(s)	3461
BtsIMutI	CAGTG	6 cut(s)	121, 2003, 2895, 3218, 3461, 4485
BtuMI	TCGCGA	1 cut(s)	3642
BveI	ACCTGC	4 cut(s)	1723, 3283, 5107, 5352
Cac8I	GCNNGC	6 cut(s)	255, 421, 822, 1975, 2296, 5260
CaiI	CAGNNNCTG	2 cut(s)	1832, 1970
CciI	TCATGA	4 cut(s)	282, 1545, 1671, 3771
CfoI	GCGC	3 cut(s)	309, 1138, 3229
Cfr10I	RCCGGY	2 cut(s)	4089, 4235
Cfr13I	GGNCC	8 cut(s)	43, 963, 1208, 1209, 1961, 2426, 3441, 4238
Cfr9I	CCCGGG	1 cut(s)	229
CseI	GACGC	4 cut(s)	40, 301, 425, 1322
CsiI	ACCWGGT	1 cut(s)	1222
Csp6I	GTAC	8 cut(s)	559, 1074, 1121, 1204, 1282, 1712, 2394, 3154
CviQI	GTAC	8 cut(s)	559, 1074, 1121, 1204, 1282, 1712, 2394, 3154
DraI	TTTAAA	2 cut(s)	486, 2965
DraIII	CACNNNGTG	1 cut(s)	3341
DrdI	GACNNNNNNGTC	2 cut(s)	295, 2433
DseDI	GACNNNNNNGTC	2 cut(s)	295, 2433
EaeI	YGGCCR	6 cut(s)	210, 255, 822, 2912, 4189, 4200
Eam1104I	CTCTTC	9 cut(s)	572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
EarI	CTCTTC	9 cut(s)	572, 653, 1046, 1947, 2246, 2927, 3537, 3582, 3876
EciI	GGCGGA	2 cut(s)	35, 824
Ecl136II	GAGCTC	1 cut(s)	1543
Eco130I	CCWWGG	7 cut(s)	39, 959, 1124, 1984, 2100, 3432, 3462
Eco147I	AGGCCT	1 cut(s)	985
Eco24I	GRGCYC	5 cut(s)	74, 1212, 1545, 2224, 4535
Eco31I	GGTCTC	1 cut(s)	757
Eco32I	GATATC	1 cut(s)	3889
Eco47I	GGWCC	3 cut(s)	43, 1961, 2426
Eco53kI	GAGCTC	1 cut(s)	1543
Eco57I	CTGAAG	6 cut(s)	1791, 2951, 2959, 3438, 3837, 3854
Eco88I	CYCGRG	2 cut(s)	229, 790
EcoICRI	GAGCTC	1 cut(s)	1543
EcoNI	CCTNNNNNAGG	2 cut(s)	790, 1221
EcoO109I	RGGNCCY	1 cut(s)	1209
EcoRI	GAATTC	2 cut(s)	2452, 3762
EcoRV	GATATC	1 cut(s)	3889
EcoT14I	CCWWGG	7 cut(s)	39, 959, 1124, 1984, 2100, 3432, 3462
EcoT22I	ATGCAT	3 cut(s)	1565, 2032, 2997
EcoT38I	GRGCYC	5 cut(s)	74, 1212, 1545, 2224, 4535
ErhI	CCWWGG	7 cut(s)	39, 959, 1124, 1984, 2100, 3432, 3462
FalI	AAGNNNNNCTT	4 cut(s)	3534, 3566, 4792, 4824
FaqI	GGGAC	9 cut(s)	187, 1555, 1930, 2358, 2623, 2645, 4378, 4660, 4716
FauI	CCCGC	6 cut(s)	142, 886, 1982, 4918, 4939, 4960
FauNDI	CATATG	2 cut(s)	1946, 2377
FbaI	TGATCA	3 cut(s)	1108, 1300, 3931
FblI	GTMKAC	1 cut(s)	3405
Fnu4HI	GCNGC	8 cut(s)	49, 310, 518, 1971, 2067, 3255, 3620, 4427
FriOI	GRGCYC	5 cut(s)	74, 1212, 1545, 2224, 4535
Fsp4HI	GCNGC	8 cut(s)	49, 310, 518, 1971, 2067, 3255, 3620, 4427
FspBI	CTAG	9 cut(s)	338, 1524, 3132, 3338, 4343, 4431, 5163, 5259, 5340
GlaI	GCGC	3 cut(s)	308, 1137, 3228
GluI	GCNGC	8 cut(s)	49, 310, 518, 1971, 2067, 3255, 3620, 4427
GsaI	CCCAGC	2 cut(s)	131, 173
GsuI	CTGGAG	9 cut(s)	269, 1781, 2174, 3014, 4389, 4406, 4461, 4599, 4746
HaeII	RGCGCY	1 cut(s)	3230
HapII	CCGG	9 cut(s)	184, 230, 1890, 2165, 2771, 4090, 4236, 4784, 5358
HgaI	GACGC	4 cut(s)	40, 301, 425, 1322
HhaI	GCGC	3 cut(s)	309, 1138, 3229
Hin1I	GRCGYC	1 cut(s)	2634
Hin6I	GCGC	3 cut(s)	307, 1136, 3227
HinP1I	GCGC	3 cut(s)	307, 1136, 3227
HincII	GTYRAC	4 cut(s)	1373, 2926, 3595, 3922
HindII	GTYRAC	4 cut(s)	1373, 2926, 3595, 3922
HpaI	GTTAAC	2 cut(s)	2926, 3922
HpaII	CCGG	9 cut(s)	184, 230, 1890, 2165, 2771, 4090, 4236, 4784, 5358
Hpy166II	GTNNAC	8 cut(s)	1373, 2366, 2926, 3272, 3406, 3595, 3922, 4144
Hpy8I	GTNNAC	8 cut(s)	1373, 2366, 2926, 3272, 3406, 3595, 3922, 4144
HpyCH4IV	ACGT	9 cut(s)	712, 1072, 1503, 1860, 1941, 2634, 3205, 4181, 4673
HpySE526I	ACGT	9 cut(s)	712, 1072, 1503, 1860, 1941, 2634, 3205, 4181, 4673
Hsp92I	GRCGYC	1 cut(s)	2634
HspAI	GCGC	3 cut(s)	307, 1136, 3227
Kpn2I	TCCGGA	2 cut(s)	1889, 2770
Ksp22I	TGATCA	3 cut(s)	1108, 1300, 3931
KspAI	GTTAAC	2 cut(s)	2926, 3922
LguI	GCTCTTC	1 cut(s)	3876
LmnI	GCTCC	9 cut(s)	250, 332, 352, 1192, 1312, 1330, 1540, 1723, 2155
Lsp1109I	GCAGC	7 cut(s)	296, 529, 1957, 2078, 3241, 3606, 4413
MabI	ACCWGGT	1 cut(s)	1222
MaeI	CTAG	9 cut(s)	338, 1524, 3132, 3338, 4343, 4431, 5163, 5259, 5340
MaeII	ACGT	9 cut(s)	712, 1072, 1503, 1860, 1941, 2634, 3205, 4181, 4673
MbiI	CCGCTC	1 cut(s)	881
MfeI	CAATTG	3 cut(s)	177, 2055, 4359
MflI	RGATCY	6 cut(s)	1090, 2800, 3520, 3547, 3563, 4133
MlsI	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
MluI	ACGCGT	1 cut(s)	417
MluNI	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
MlyI	GAGTC	2 cut(s)	1159, 3209
MmeI	TCCRAC	8 cut(s)	1007, 1518, 3126, 3951, 4766, 4799, 4883, 5177
Mox20I	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
Mph1103I	ATGCAT	3 cut(s)	1565, 2032, 2997
MroI	TCCGGA	2 cut(s)	1889, 2770
MroXI	GAANNNNTTC	2 cut(s)	597, 642
MscI	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
MslI	CAYNNNNRTG	6 cut(s)	1341, 1550, 1682, 3178, 3284, 4014
Msp20I	TGGCCA	5 cut(s)	257, 824, 2914, 4191, 4202
MspA1I	CMGCKG	4 cut(s)	635, 820, 2294, 4426
MspCI	CTTAAG	2 cut(s)	1010, 2276
MspI	CCGG	9 cut(s)	184, 230, 1890, 2165, 2771, 4090, 4236, 4784, 5358
MunI	CAATTG	3 cut(s)	177, 2055, 4359
Mva1269I	GAATGC	5 cut(s)	2032, 2953, 3094, 4414, 4595
MvnI	CGCG	4 cut(s)	51, 419, 3642, 4471
NciI	CCSGG	4 cut(s)	230, 231, 2166, 5358
NcoI	CCATGG	1 cut(s)	1124
NdeI	CATATG	2 cut(s)	1946, 2377
NheI	GCTAGC	1 cut(s)	5258
NlaIV	GGNNCC	6 cut(s)	245, 1210, 1211, 1554, 2157, 4356
NmeAIII	GCCGAG	1 cut(s)	145
NmuCI	GTSAC	7 cut(s)	158, 1162, 1453, 2893, 3201, 4512, 5250
NruI	TCGCGA	1 cut(s)	3642
NsiI	ATGCAT	3 cut(s)	1565, 2032, 2997
NspI	RCATGY	1 cut(s)	2795
NspV	TTCGAA	2 cut(s)	3760, 4260
PaeR7I	CTCGAG	1 cut(s)	790
PagI	TCATGA	4 cut(s)	282, 1545, 1671, 3771
PaqCI	CACCTGC	1 cut(s)	5107
PceI	AGGCCT	1 cut(s)	985
PciI	ACATGT	1 cut(s)	2791
PciSI	GCTCTTC	1 cut(s)	3876
PctI	GAATGC	5 cut(s)	2032, 2953, 3094, 4414, 4595
PdmI	GAANNNNTTC	2 cut(s)	597, 642
Pfl23II	CGTACG	1 cut(s)	1073
PflFI	GACNNNGTC	1 cut(s)	1355
PflMI	CCANNNNNTGG	2 cut(s)	688, 4198
PfoI	TCCNGGA	1 cut(s)	3030
PkrI	GCNGC	8 cut(s)	50, 311, 519, 1972, 2068, 3256, 3621, 4428
PleI	GAGTC	2 cut(s)	1159, 3208
PpsI	GAGTC	2 cut(s)	1159, 3208
Ppu21I	YACGTR	2 cut(s)	1073, 1861
PscI	ACATGT	1 cut(s)	2791
PshBI	ATTAAT	2 cut(s)	3366, 3536
PsiI	TTATAA	4 cut(s)	1173, 1323, 1467, 1775
Psp124BI	GAGCTC	1 cut(s)	1545
PspFI	CCCAGC	2 cut(s)	127, 169
PspLI	CGTACG	1 cut(s)	1073
PspN4I	GGNNCC	6 cut(s)	245, 1210, 1211, 1554, 2157, 4356
PspOMI	GGGCCC	1 cut(s)	1208
PspPI	GGNCC	8 cut(s)	43, 963, 1208, 1209, 1961, 2426, 3441, 4238
PspXI	VCTCGAGB	1 cut(s)	790
PstI	CTGCAG	1 cut(s)	2533
PstNI	CAGNNNCTG	2 cut(s)	1832, 1970
PsuI	RGATCY	6 cut(s)	1090, 2800, 3520, 3547, 3563, 4133
PsyI	GACNNNGTC	1 cut(s)	1355
PvuII	CAGCTG	3 cut(s)	820, 2294, 4426
RruI	TCGCGA	1 cut(s)	3642
RsaI	GTAC	8 cut(s)	560, 1075, 1122, 1205, 1283, 1713, 2395, 3155
RsaNI	GTAC	8 cut(s)	559, 1074, 1121, 1204, 1282, 1712, 2394, 3154
RseI	CAYNNNNRTG	6 cut(s)	1341, 1550, 1682, 3178, 3284, 4014
SacI	GAGCTC	1 cut(s)	1545
SapI	GCTCTTC	1 cut(s)	3876
SatI	GCNGC	8 cut(s)	49, 310, 518, 1971, 2067, 3255, 3620, 4427
Sau96I	GGNCC	8 cut(s)	43, 963, 1208, 1209, 1961, 2426, 3441, 4238
SchI	GAGTC	2 cut(s)	1159, 3209
SexAI	ACCWGGT	1 cut(s)	1222
Sfr274I	CTCGAG	1 cut(s)	790
SfuI	TTCGAA	2 cut(s)	3760, 4260
SinI	GGWCC	3 cut(s)	43, 1961, 2426
SlaI	CTCGAG	1 cut(s)	790
SmaI	CCCGGG	1 cut(s)	231
SmiMI	CAYNNNNRTG	6 cut(s)	1341, 1550, 1682, 3178, 3284, 4014
SmlI	CTYRAG	3 cut(s)	790, 1010, 2276
SmoI	CTYRAG	3 cut(s)	790, 1010, 2276
SseBI	AGGCCT	1 cut(s)	985
SspI	AATATT	2 cut(s)	1177, 1728
SspMI	CTAG	9 cut(s)	338, 1524, 3132, 3338, 4343, 4431, 5163, 5259, 5340
SstI	GAGCTC	1 cut(s)	1545
StuI	AGGCCT	1 cut(s)	985
StyI	CCWWGG	7 cut(s)	39, 959, 1124, 1984, 2100, 3432, 3462
TaiI	ACGT	9 cut(s)	715, 1075, 1506, 1863, 1944, 2637, 3208, 4184, 4676
TaqI	TCGA	7 cut(s)	791, 1438, 2464, 2970, 3760, 3874, 4260
TatI	WGTACW	2 cut(s)	558, 3153
TauI	GCSGC	1 cut(s)	51
TscAI	CASTG	6 cut(s)	121, 2003, 2902, 3225, 3461, 4485
TseFI	GTSAC	7 cut(s)	158, 1162, 1453, 2893, 3201, 4512, 5250
TseI	GCWGC	7 cut(s)	309, 517, 1970, 2066, 3254, 3619, 4426
Tsp45I	GTSAC	7 cut(s)	158, 1162, 1453, 2893, 3201, 4512, 5250
TspGWI	ACGGA	4 cut(s)	885, 3603, 4322, 4794
TspMI	CCCGGG	1 cut(s)	229
TspRI	CASTG	6 cut(s)	121, 2003, 2902, 3225, 3461, 4485
Tth111I	GACNNNGTC	1 cut(s)	1355
Van91I	CCANNNNNTGG	2 cut(s)	688, 4198
Vha464I	CTTAAG	2 cut(s)	1010, 2276
VpaK11BI	GGWCC	3 cut(s)	43, 1961, 2426
VspI	ATTAAT	2 cut(s)	3366, 3536
XagI	CCTNNNNNAGG	2 cut(s)	790, 1221
XapI	RAATTY	7 cut(s)	408, 1979, 2452, 2620, 3606, 3701, 3762
XceI	RCATGY	1 cut(s)	2795
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1743
XhoI	CTCGAG	1 cut(s)	790
XmaI	CCCGGG	1 cut(s)	229
XmiI	GTMKAC	1 cut(s)	3405
XmnI	GAANNNNTTC	2 cut(s)	597, 642
XspI	CTAG	9 cut(s)	338, 1524, 3132, 3338, 4343, 4431, 5163, 5259, 5340
ZraI	GACGTC	1 cut(s)	2635
Zsp2I	ATGCAT	3 cut(s)	1565, 2032, 2997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.