MD02G1154800.v1.1

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002238
Physical Location & Seq
Forward (+)
12818010 .. 12820911
2902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1154800.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGATGGGAGGAAAATCCAGTAAAAACCTTGAAAACCTTGAAACTACTACCATCTCCCAGGACAGCGCCGATTTGAGCTCCTATGAGGCAGCTTGTAAGCTCGATCCAGCCTTGCAATCGTTTGATGTCAATCTCCAGCAGCGCACAAGCCGTGTCATTCACTCGCTTTCAGCTGGGTTGGATTTTCGGTCCCTATCCCTTGACTCGCTCAAGGAGGTTACCGATTGCTTGCTTGACATGAACCAGGAAGTGGTCAAGGTGATTCTAGAATGCAAGAAGGATATATGGAAAAGTAAAGAAATGTTCTCTCTTGTGGAGGAATACTTTGAGAACAGTCTTCAAACGTTGGATTTTTGTACTTCCCTCGAGCAATGTCTTAAGCGTGCGCTCAATACCCAGCTGATAATTCAAGCTGCAGTTAGGCAGTTTGAGGAAGAAGTAGGTGCCGGGGCAGATGGGAACGGGTGTGCAAGAACCTTAAAAGAATTGAGGGCGTTTAAGGCGGCTGGGGATCCTTTCACGGACGAGTTCTTTATACTGTTTCAAGCAGTTTACAAGAACCAGGCATCCATGTTTGAGAAACTGCAACTTCGTAAGAGAAAACTTGATAAGAAGTTCAAATCAATGAAAGCTTGGAGGAGGCTGTCAAATGTTATTTTCGTTGCTACATTTGTCTCAGTATTGGTTTTCTCTGTAGTGGCAGCTGCCATTGCTGCACCACCACTTGTAACGGCCCTGGCAGGAGCATTGGCTATCCCAATAGGTTCAGTTGGAAAATGGTGTAATATTCTTTGGAATAACTACGAGAAAGGACTGAAAGGGCAGAGGGAGATAATCAGCTCAATGCAGATTCGAACGTTCGTCACAATGAAGGACCTGGATAGCATCCGGGTGCTTGTTGACAAATTTGAAATTGGGATTGAGTCACTGTTGCACAATGTTGATTTTGCTATTAGAGAAGAGGGGTCGGCTGTGAAGCTAGTGATGGATGAGATCAAGAAAAAGCTTGATGTGTTTGTGGAAACCATTGATAATCTAAGCCAGCATGCTGATAAGTGTAGCCGGGATATAAGGAAGGCAAGGACAGTGATTTCGCAGAGAATAATTGGGTATCCCAACAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

375

Amino Acids

42.03

Weight (kDa)

8.3

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 37 - 149 1.1e-06 Protein ROH1-like
DUF677 PF05055 38 - 368 6.3e-129 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 250
AciI CCGC 1 cut(s) 503
AclI AACGTT 2 cut(s) 344, 857
AclWI GGATC 3 cut(s) 98, 506, 519
AcsI RAATTY 1 cut(s) 905
AfaI GTAC 1 cut(s) 358
AfiI CCNNNNNNNGG 1 cut(s) 250
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 7 cut(s) 32, 41, 341, 410, 545, 619, 911
AjnI CCWGG 5 cut(s) 57, 243, 561, 735, 876
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 3 cut(s) 98, 506, 519
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 1 cut(s) 732
ApeKI GCWGC 6 cut(s) 89, 139, 413, 701, 704, 713
ApoI RAATTY 1 cut(s) 905
ArsI GACNNNNNNTTYG 2 cut(s) 1075, 1107
AspLEI GCGC 3 cut(s) 68, 144, 388
AspS9I GGNCC 3 cut(s) 189, 733, 874
AsuC2I CCSGG 3 cut(s) 448, 890, 1064
AsuHPI GGTGA 1 cut(s) 271
AsuII TTCGAA 1 cut(s) 853
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 2 cut(s) 189, 874
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 6 cut(s) 101, 151, 400, 691, 700, 713
BccI CCATC 3 cut(s) 59, 449, 979
BceAI ACGGC 2 cut(s) 135, 747
BciT130I CCWGG 5 cut(s) 59, 245, 563, 737, 878
BciVI GTATCC 1 cut(s) 1122
BcnI CCSGG 3 cut(s) 448, 890, 1064
BcoDI GTCTC 1 cut(s) 679
BfaI CTAG 2 cut(s) 266, 980
BfmI CTRYAG 2 cut(s) 414, 693
BfoI RGCGCY 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 377
BfuI GTATCC 1 cut(s) 1122
BisI GCNGC 7 cut(s) 90, 140, 414, 504, 702, 705, 714
BlsI GCNGC 7 cut(s) 91, 141, 415, 505, 703, 706, 715
Bme1390I CCNGG 8 cut(s) 59, 245, 448, 563, 737, 878, 890, 1064
Bme18I GGWCC 2 cut(s) 189, 874
BmeT110I CYCGRG 1 cut(s) 365
BmgT120I GGNCC 3 cut(s) 189, 733, 874
BmiI GGNNCC 3 cut(s) 191, 445, 513
BmrFI CCNGG 8 cut(s) 59, 245, 448, 563, 737, 878, 890, 1064
BmsI GCATC 2 cut(s) 575, 894
BpiI GAAGAC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 853
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 3 cut(s) 448, 890, 1064
BsaBI GATNNNNATC 1 cut(s) 129
BsaJI CCNNGG 3 cut(s) 57, 447, 735
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 2 cut(s) 377, 708
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 5 cut(s) 59, 245, 563, 737, 878
BseDI CCNNGG 3 cut(s) 57, 447, 735
BseGI GGATG 3 cut(s) 566, 885, 994
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 2 cut(s) 377, 708
BseMII CTCAG 1 cut(s) 690
BseNI ACTGG 1 cut(s) 18
BseRI GAGGAG 1 cut(s) 652
BseXI GCAGC 6 cut(s) 101, 151, 400, 691, 700, 713
BseYI CCCAGC 3 cut(s) 173, 396, 506
BsgI GTGCAG 1 cut(s) 699
BshFI GGCC 1 cut(s) 734
BshNI GGYRCC 1 cut(s) 443
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 3 cut(s) 447, 889, 1063
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 679
BsmFI GGGAC 1 cut(s) 175
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 734
BsoBI CYCGRG 1 cut(s) 365
Bsp119I TTCGAA 1 cut(s) 853
Bsp1286I GDGCHC 1 cut(s) 80
Bsp143I GATC 3 cut(s) 103, 511, 993
BspACI CCGC 1 cut(s) 503
BspANI GGCC 1 cut(s) 734
BspCNI CTCAG 1 cut(s) 689
BspLI GGNNCC 3 cut(s) 191, 445, 513
BspMAI CTGCAG 1 cut(s) 418
BspPI GGATC 3 cut(s) 98, 506, 519
BspT104I TTCGAA 1 cut(s) 853
BspT107I GGYRCC 1 cut(s) 443
BspTI CTTAAG 1 cut(s) 377
BsrDI GCAATG 2 cut(s) 377, 708
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 3 cut(s) 57, 447, 735
BssMI GATC 3 cut(s) 103, 511, 993
Bst2UI CCWGG 5 cut(s) 59, 245, 563, 737, 878
Bst4CI ACNGT 4 cut(s) 335, 540, 930, 1087
Bst6I CTCTTC 1 cut(s) 954
BstAFI CTTAAG 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 853
BstC8I GCNNGC 4 cut(s) 230, 384, 1043, 1047
BstDEI CTNAG 2 cut(s) 676, 1037
BstEII GGTNACC 1 cut(s) 217
BstF5I GGATG 3 cut(s) 566, 885, 994
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 144, 388
BstKTI GATC 3 cut(s) 106, 514, 996
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 3 cut(s) 103, 511, 993
BstMWI GCNNNNNNNGC 3 cut(s) 500, 710, 713
BstNI CCWGG 5 cut(s) 59, 245, 563, 737, 878
BstNSI RCATGY 1 cut(s) 1049
BstPI GGTNACC 1 cut(s) 217
BstSCI CCNGG 8 cut(s) 57, 243, 446, 561, 735, 876, 888, 1062
BstSFI CTRYAG 2 cut(s) 414, 693
BstV1I GCAGC 6 cut(s) 101, 151, 400, 691, 700, 713
BstV2I GAAGAC 1 cut(s) 329
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuI GTATCC 1 cut(s) 1122
BsuRI GGCC 1 cut(s) 734
BtsCI GGATG 3 cut(s) 566, 885, 994
BtsIMutI CAGTG 2 cut(s) 926, 1092
Cac8I GCNNGC 4 cut(s) 230, 384, 1043, 1047
CfoI GCGC 3 cut(s) 68, 144, 388
Cfr13I GGNCC 3 cut(s) 189, 733, 874
Csp6I GTAC 1 cut(s) 357
CviAII CATG 3 cut(s) 238, 571, 1046
CviQI GTAC 1 cut(s) 357
DdeI CTNAG 2 cut(s) 676, 1037
DpnI GATC 3 cut(s) 105, 513, 995
DpnII GATC 3 cut(s) 103, 511, 993
Eam1104I CTCTTC 1 cut(s) 954
EarI CTCTTC 1 cut(s) 954
Ecl136II GAGCTC 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 189, 874
Eco53kI GAGCTC 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 365
Eco91I GGTNACC 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 874
EcoO65I GGTNACC 1 cut(s) 217
EcoRII CCWGG 5 cut(s) 57, 243, 561, 735, 876
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 3 cut(s) 241, 574, 1049
FaiI YATR 8 cut(s) 84, 239, 284, 286, 536, 572, 1047, 1070
FaqI GGGAC 1 cut(s) 175
FatI CATG 3 cut(s) 237, 570, 1045
Fnu4HI GCNGC 7 cut(s) 90, 140, 414, 504, 702, 705, 714
FokI GGATG 3 cut(s) 553, 872, 1001
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 7 cut(s) 90, 140, 414, 504, 702, 705, 714
FspBI CTAG 2 cut(s) 266, 980
GlaI GCGC 3 cut(s) 67, 143, 387
GluI GCNGC 7 cut(s) 90, 140, 414, 504, 702, 705, 714
GsaI CCCAGC 3 cut(s) 177, 400, 510
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 734
HapII CCGG 3 cut(s) 447, 889, 1063
HhaI GCGC 3 cut(s) 68, 144, 388
Hin1II CATG 3 cut(s) 241, 574, 1049
Hin6I GCGC 3 cut(s) 66, 142, 386
HinP1I GCGC 3 cut(s) 66, 142, 386
HincII GTYRAC 1 cut(s) 901
HindII GTYRAC 1 cut(s) 901
HindIII AAGCTT 2 cut(s) 630, 1004
HinfI GANTC 4 cut(s) 203, 262, 850, 923
HpaII CCGG 3 cut(s) 447, 889, 1063
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 2 cut(s) 553, 901
Hpy188III TCNNGA 2 cut(s) 266, 997
Hpy8I GTNNAC 2 cut(s) 553, 901
HpyAV CCTTC 3 cut(s) 271, 865, 1069
HpyCH4III ACNGT 4 cut(s) 335, 540, 930, 1087
HpyCH4IV ACGT 2 cut(s) 344, 857
HpyCH4V TGCA 8 cut(s) 115, 273, 416, 470, 586, 716, 847, 934
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 710, 713
HpyF3I CTNAG 2 cut(s) 676, 1037
HpySE526I ACGT 2 cut(s) 344, 857
Hsp92II CATG 3 cut(s) 241, 574, 1049
HspAI GCGC 3 cut(s) 66, 142, 386
Kzo9I GATC 3 cut(s) 103, 511, 993
LmnI GCTCC 2 cut(s) 83, 743
Lsp1109I GCAGC 6 cut(s) 101, 151, 400, 691, 700, 713
LweI GCATC 2 cut(s) 575, 894
MaeI CTAG 2 cut(s) 266, 980
MaeII ACGT 2 cut(s) 344, 857
MaeIII GTNAC 4 cut(s) 217, 727, 862, 924
MalI GATC 3 cut(s) 105, 513, 995
MboI GATC 3 cut(s) 103, 511, 993
MboII GAAGA 3 cut(s) 329, 446, 971
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 1 cut(s) 80
MluCI AATT 5 cut(s) 405, 485, 905, 912, 1104
MlyI GAGTC 2 cut(s) 197, 932
MmeI TCCRAC 3 cut(s) 159, 327, 751
MseI TTAA 3 cut(s) 378, 479, 498
MslI CAYNNNNRTG 1 cut(s) 890
MspA1I CMGCKG 3 cut(s) 173, 400, 704
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 3 cut(s) 447, 889, 1063
MspR9I CCNGG 8 cut(s) 59, 245, 448, 563, 737, 878, 890, 1064
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 5 cut(s) 59, 245, 563, 737, 878
MwoI GCNNNNNNNGC 3 cut(s) 500, 710, 713
NciI CCSGG 3 cut(s) 448, 890, 1064
NdeII GATC 3 cut(s) 103, 511, 993
NlaIII CATG 3 cut(s) 241, 574, 1049
NlaIV GGNNCC 3 cut(s) 191, 445, 513
NmuCI GTSAC 2 cut(s) 862, 924
NspI RCATGY 1 cut(s) 1049
NspV TTCGAA 1 cut(s) 853
PaeI GCATGC 1 cut(s) 1049
PaeR7I CTCGAG 1 cut(s) 365
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 2 cut(s) 262, 850
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 7 cut(s) 91, 141, 415, 505, 703, 706, 715
PleI GAGTC 2 cut(s) 197, 931
PpsI GAGTC 2 cut(s) 197, 931
PpuMI RGGWCCY 1 cut(s) 874
Psp124BI GAGCTC 1 cut(s) 80
Psp1406I AACGTT 2 cut(s) 344, 857
Psp5II RGGWCCY 1 cut(s) 874
Psp6I CCWGG 5 cut(s) 57, 243, 561, 735, 876
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 3 cut(s) 173, 396, 506
PspGI CCWGG 5 cut(s) 57, 243, 561, 735, 876
PspN4I GGNNCC 3 cut(s) 191, 445, 513
PspPI GGNCC 3 cut(s) 189, 733, 874
PspPPI RGGWCCY 1 cut(s) 874
PspXI VCTCGAGB 1 cut(s) 365
PstI CTGCAG 1 cut(s) 418
PsuI RGATCY 1 cut(s) 511
PvuII CAGCTG 3 cut(s) 173, 400, 704
RsaI GTAC 1 cut(s) 358
RsaNI GTAC 1 cut(s) 357
RseI CAYNNNNRTG 1 cut(s) 890
SacI GAGCTC 1 cut(s) 80
SaqAI TTAA 3 cut(s) 378, 479, 498
SatI GCNGC 7 cut(s) 90, 140, 414, 504, 702, 705, 714
Sau3AI GATC 3 cut(s) 103, 511, 993
Sau96I GGNCC 3 cut(s) 189, 733, 874
SchI GAGTC 2 cut(s) 197, 932
ScrFI CCNGG 8 cut(s) 59, 245, 448, 563, 737, 878, 890, 1064
SduI GDGCHC 1 cut(s) 80
SfaNI GCATC 2 cut(s) 575, 894
SfcI CTRYAG 2 cut(s) 414, 693
Sfr274I CTCGAG 1 cut(s) 365
SfuI TTCGAA 1 cut(s) 853
SinI GGWCC 2 cut(s) 189, 874
SlaI CTCGAG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 3 cut(s) 209, 365, 377
SmoI CTYRAG 3 cut(s) 209, 365, 377
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 5 cut(s) 405, 485, 905, 912, 1104
SsiI CCGC 1 cut(s) 503
SspI AATATT 1 cut(s) 787
SspMI CTAG 2 cut(s) 266, 980
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 8 cut(s) 57, 243, 446, 561, 735, 876, 888, 1062
TaaI ACNGT 4 cut(s) 335, 540, 930, 1087
TaiI ACGT 2 cut(s) 347, 860
TaqI TCGA 3 cut(s) 102, 366, 853
TaqII GACCGA 1 cut(s) 177
TasI AATT 5 cut(s) 405, 485, 905, 912, 1104
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 506
TfiI GAWTC 2 cut(s) 262, 850
Tru1I TTAA 3 cut(s) 378, 479, 498
Tru9I TTAA 3 cut(s) 378, 479, 498
TscAI CASTG 2 cut(s) 933, 1092
TseFI GTSAC 2 cut(s) 862, 924
TseI GCWGC 6 cut(s) 89, 139, 413, 701, 704, 713
Tsp45I GTSAC 2 cut(s) 862, 924
TspDTI ATGAA 3 cut(s) 254, 641, 884
TspGWI ACGGA 1 cut(s) 536
TspRI CASTG 2 cut(s) 933, 1092
Van91I CCANNNNNTGG 1 cut(s) 250
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 189, 874
XapI RAATTY 1 cut(s) 905
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 1 cut(s) 1049
XhoI CTCGAG 1 cut(s) 365
XspI CTAG 2 cut(s) 266, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.