RchiOBHm_Chr2g0102731

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002238
Physical Location & Seq
Forward (+)
14371590 .. 14375333
3744 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47714

Sequence Viewer

Length: 1167 bp
ATGATGGGAGGAAACTGCAGTAAAAACAAAGGTGTTGATGCTCCACCACCCCCGCCGCCGCCGCCGCCGCCACAACAACCCCTCGAAAGCAATACCAAATCCGAGTACACAGCCCATTTGAGCTCTTATGAGGCAGCCTGTAAGCTCGATTCGGGTTTACAATCGTTTGATGTATCTCTCCAACAGCGCACTAGCCGAGTCTTCAACTCACTTTCCACTGGAGTTGAAGTTAGGTCCCTATCTTTGGACTCACTCAAAGAGGTCACTGATTGTTTGCTTGAGATGAACCAGGAAGTGGTCAGTGTGATTCTCGAATGCAAGAAGGATGTATGGAACAATCAAGAGATGTTCTCTCTAGTGGAGGAGTACTTTGAGAACAGTCTCCAGACATTGGACTTCTGTACTGCCCTCGAGAGATGTCTCAAGCGTGCAGTGAACAATCAGCTGATAATTCAGGCTGCGGTGAGGCAGTTTGAGGAAGAAGTAGAAGCTGGTTTCGAAGGGAAGGGGTGTGTGAGGACATTGCAGGAGTTGAGGGCATTTAAGGCAGCCGGGGACCCTTTCACGGAGGAGTTCTTTGTACTATTTCAGGCAGTTTATAACCAACAGGCATCGATGCTCCAGAAATTGCAAGTCCGAAAGAGAAAGCTTGATAAGAAGTTGAAATCAGTGAAAGCTTGGCGGAAAGTGTCGAATGTTATTTTTGTTGCCACTTTTGTGTCTGTATTGATATTTTCGGTGGTGGCAGCTGCCATTGCTGCACCGCCACTTGTAACCGCCTTGGCAGGTGCACTGGCTGTTCCGATAGGTTCTGTTGGAAAATGGTGTAATATGCTTTGGAGCCGCTACGAGAGGGCTCTGAAAGGGCAGAGGGAGATAATAAGCTCAATGCAGGTTGGAAGTATGATTACAATGACAGACTTGAACAACATCAGGGTGCTTGTTGAGAAATTTGAAATCGAGATTGAGGCACTGCTTCAGAATGCTGACTTTGCTATTAGAGAAGAGGATGCTGTGAAGATCGTGATGATTGAGATCAACAAAAAGCTGGAAAAGTTTATGGAAACCATCGAAAGTTTAAGCCAAAACGCTGATAAGTGTGGCCGGAATATAAGGAAGGCAAGGACAGTGATTTTGCAGAGAATAATTAGGCATCCCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

388

Amino Acids

43.58

Weight (kDa)

8.28

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 53 - 382 1.8e-131 Protein of unknown function (DUF677)
ROH1-like PF05633 54 - 161 6.1e-07 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AarI CACCTGC 1 cut(s) 776
Acc36I ACCTGC 2 cut(s) 776, 883
AccB7I CCANNNNNTGG 2 cut(s) 295, 391
AcoI YGGCCR 1 cut(s) 1102
AcsI RAATTY 1 cut(s) 950
AcuI CTGAAG 1 cut(s) 962
AfaI GTAC 4 cut(s) 107, 368, 403, 582
AfiI CCNNNNNNNGG 4 cut(s) 244, 295, 391, 565
AgsI TTSAA 5 cut(s) 205, 227, 664, 925, 956
AjnI CCWGG 1 cut(s) 288
AleI CACNNNNGTG 1 cut(s) 716
AluBI AGCT 9 cut(s) 123, 145, 445, 491, 649, 677, 749, 885, 1048
AluI AGCT 9 cut(s) 123, 145, 445, 491, 649, 677, 749, 885, 1048
Alw21I GWGCWC 2 cut(s) 125, 793
Alw26I GTCTC 2 cut(s) 386, 425
Alw44I GTGCAC 1 cut(s) 789
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 1 cut(s) 1102
ApaLI GTGCAC 1 cut(s) 789
ApeKI GCWGC 6 cut(s) 134, 458, 548, 746, 749, 758
ApoI RAATTY 1 cut(s) 950
ArsI GACNNNNNNTTYG 2 cut(s) 1117, 1149
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 234, 556
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 1 cut(s) 475
AsuII TTCGAA 1 cut(s) 498
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 2 cut(s) 234, 556
BaeGI GKGCMC 1 cut(s) 793
BanII GRGCYC 2 cut(s) 125, 859
BarI GAAGNNNNNNTAC 2 cut(s) 892, 924
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 125, 793
BbvI GCAGC 6 cut(s) 146, 445, 560, 736, 745, 758
BccI CCATC 2 cut(s) 1076, 1166
BciT130I CCWGG 1 cut(s) 290
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 2 cut(s) 386, 425
BfaI CTAG 2 cut(s) 192, 356
BfmI CTRYAG 1 cut(s) 16
BfuAI ACCTGC 2 cut(s) 776, 883
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 290, 553
Bme18I GGWCC 2 cut(s) 234, 556
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 2 cut(s) 234, 556
BmiI GGNNCC 4 cut(s) 236, 557, 558, 842
BmrFI CCNGG 2 cut(s) 290, 553
BmsI GCATC 5 cut(s) 28, 606, 620, 1000, 1162
BpiI GAAGAC 1 cut(s) 193
BplI GAGNNNNNCTC 2 cut(s) 335, 367
BpmI CTGGAG 3 cut(s) 240, 368, 605
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 2 cut(s) 299, 407
BpuMI CCSGG 1 cut(s) 553
Bsa29I ATCGAT 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 1034
BsaJI CCNNGG 2 cut(s) 552, 780
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 295, 391, 565
Bse1I ACTGG 2 cut(s) 223, 798
Bse3DI GCAATG 2 cut(s) 521, 753
Bse8I GATNNNNATC 1 cut(s) 1034
BseBI CCWGG 1 cut(s) 290
BseCI ATCGAT 1 cut(s) 614
BseDI CCNNGG 2 cut(s) 552, 780
BseGI GGATG 3 cut(s) 331, 1015, 1153
BseJI GATNNNNATC 1 cut(s) 1034
BseLI CCNNNNNNNGG 4 cut(s) 244, 295, 391, 565
BseMI GCAATG 2 cut(s) 521, 753
BseNI ACTGG 2 cut(s) 223, 798
BseRI GAGGAG 2 cut(s) 377, 584
BseSI GKGCMC 1 cut(s) 793
BseXI GCAGC 6 cut(s) 146, 445, 560, 736, 745, 758
BsgI GTGCAG 2 cut(s) 450, 744
BshFI GGCC 1 cut(s) 1104
BshVI ATCGAT 1 cut(s) 614
BsiHKAI GWGCWC 2 cut(s) 125, 793
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 2 cut(s) 552, 1105
BslFI GGGAC 2 cut(s) 220, 569
BslI CCNNNNNNNGG 4 cut(s) 244, 295, 391, 565
BsmAI GTCTC 2 cut(s) 386, 425
BsmFI GGGAC 2 cut(s) 220, 569
BsmI GAATGC 2 cut(s) 320, 988
BsnI GGCC 1 cut(s) 1104
BsoBI CYCGRG 1 cut(s) 410
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 3 cut(s) 125, 793, 859
Bsp143I GATC 2 cut(s) 1020, 1035
BspANI GGCC 1 cut(s) 1104
BspDI ATCGAT 1 cut(s) 614
BspLI GGNNCC 4 cut(s) 236, 557, 558, 842
BspMAI CTGCAG 1 cut(s) 20
BspMI ACCTGC 2 cut(s) 776, 883
BspT104I TTCGAA 1 cut(s) 498
BsrDI GCAATG 2 cut(s) 521, 753
BsrI ACTGG 2 cut(s) 223, 798
BssECI CCNNGG 2 cut(s) 552, 780
BssMI GATC 2 cut(s) 1020, 1035
BssT1I CCWWGG 1 cut(s) 780
Bst2UI CCWGG 1 cut(s) 290
Bst4CI ACNGT 2 cut(s) 380, 1129
Bst6I CTCTTC 1 cut(s) 999
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 429
BstF5I GGATG 3 cut(s) 331, 1015, 1153
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 2 cut(s) 1023, 1038
BstMAI GTCTC 2 cut(s) 386, 425
BstMBI GATC 2 cut(s) 1020, 1035
BstMWI GCNNNNNNNGC 7 cut(s) 61, 64, 67, 545, 755, 758, 992
BstNI CCWGG 1 cut(s) 290
BstSCI CCNGG 2 cut(s) 288, 551
BstSFI CTRYAG 1 cut(s) 16
BstSLI GKGCMC 1 cut(s) 793
BstV1I GCAGC 6 cut(s) 146, 445, 560, 736, 745, 758
BstV2I GAAGAC 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 614
BsuRI GGCC 1 cut(s) 1104
BsuTUI ATCGAT 1 cut(s) 614
BtsCI GGATG 3 cut(s) 331, 1015, 1153
BtsI GCAGTG 2 cut(s) 438, 971
BtsIMutI CAGTG 8 cut(s) 216, 264, 307, 438, 675, 791, 971, 1134
BveI ACCTGC 2 cut(s) 776, 883
Cac8I GCNNGC 1 cut(s) 429
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 234, 556
ClaI ATCGAT 1 cut(s) 614
Csp6I GTAC 4 cut(s) 106, 367, 402, 581
CspCI CAANNNNNGTGG 2 cut(s) 205, 240
CviQI GTAC 4 cut(s) 106, 367, 402, 581
DpnI GATC 2 cut(s) 1022, 1037
DpnII GATC 2 cut(s) 1020, 1035
EaeI YGGCCR 1 cut(s) 1102
Eam1104I CTCTTC 1 cut(s) 999
EarI CTCTTC 1 cut(s) 999
EciI GGCGGA 1 cut(s) 697
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 780
Eco24I GRGCYC 2 cut(s) 125, 859
Eco47I GGWCC 2 cut(s) 234, 556
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 962
Eco88I CYCGRG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 234, 556
EcoRII CCWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 780
EcoT38I GRGCYC 2 cut(s) 125, 859
ErhI CCWWGG 1 cut(s) 780
FaiI YATR 7 cut(s) 129, 331, 600, 833, 905, 1061, 1112
FaqI GGGAC 2 cut(s) 220, 569
FauI CCCGC 1 cut(s) 60
FokI GGATG 3 cut(s) 338, 1022, 1140
FriOI GRGCYC 2 cut(s) 125, 859
FspBI CTAG 2 cut(s) 192, 356
GlaI GCGC 1 cut(s) 188
GsuI CTGGAG 3 cut(s) 240, 368, 605
HaeIII GGCC 1 cut(s) 1104
HapII CCGG 2 cut(s) 552, 1105
HhaI GCGC 1 cut(s) 189
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 2 cut(s) 647, 675
HinfI GANTC 4 cut(s) 149, 198, 248, 307
HpaII CCGG 2 cut(s) 552, 1105
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 4 cut(s) 108, 158, 436, 791
Hpy188I TCNGA 5 cut(s) 103, 638, 804, 861, 981
Hpy188III TCNNGA 7 cut(s) 311, 341, 385, 412, 622, 961, 1024
Hpy8I GTNNAC 4 cut(s) 108, 158, 436, 791
HpyAV CCTTC 4 cut(s) 316, 494, 499, 1111
HpyCH4III ACNGT 2 cut(s) 380, 1129
HpyCH4V TGCA 9 cut(s) 18, 318, 431, 526, 631, 761, 791, 892, 1138
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 64, 67, 545, 755, 758, 992
HspAI GCGC 1 cut(s) 187
KflI GGGWCCC 1 cut(s) 556
Kzo9I GATC 2 cut(s) 1020, 1035
LmnI GCTCC 3 cut(s) 46, 624, 840
Lsp1109I GCAGC 6 cut(s) 146, 445, 560, 736, 745, 758
LweI GCATC 5 cut(s) 28, 606, 620, 1000, 1162
MaeI CTAG 2 cut(s) 192, 356
MaeIII GTNAC 2 cut(s) 262, 772
MalI GATC 2 cut(s) 1022, 1037
MboI GATC 2 cut(s) 1020, 1035
MboII GAAGA 4 cut(s) 193, 491, 1016, 1030
MhlI GDGCHC 3 cut(s) 125, 793, 859
MluCI AATT 4 cut(s) 450, 626, 950, 1146
MlyI GAGTC 2 cut(s) 207, 242
MmeI TCCRAC 3 cut(s) 205, 796, 877
MseI TTAA 2 cut(s) 543, 1079
MslI CAYNNNNRTG 2 cut(s) 716, 935
MspA1I CMGCKG 2 cut(s) 445, 749
MspI CCGG 2 cut(s) 552, 1105
MspR9I CCNGG 2 cut(s) 290, 553
Mva1269I GAATGC 2 cut(s) 320, 988
MvaI CCWGG 1 cut(s) 290
MwoI GCNNNNNNNGC 7 cut(s) 61, 64, 67, 545, 755, 758, 992
NciI CCSGG 1 cut(s) 553
NdeII GATC 2 cut(s) 1020, 1035
NlaIV GGNNCC 4 cut(s) 236, 557, 558, 842
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 1 cut(s) 262
NspV TTCGAA 1 cut(s) 498
OliI CACNNNNGTG 1 cut(s) 716
PaeR7I CTCGAG 1 cut(s) 410
PaqCI CACCTGC 1 cut(s) 776
PctI GAATGC 2 cut(s) 320, 988
PfeI GAWTC 2 cut(s) 149, 307
PflMI CCANNNNNTGG 2 cut(s) 295, 391
PleI GAGTC 2 cut(s) 206, 242
PpsI GAGTC 2 cut(s) 206, 242
PpuMI RGGWCCY 2 cut(s) 234, 556
PsiI TTATAA 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 234, 556
Psp6I CCWGG 1 cut(s) 288
PspGI CCWGG 1 cut(s) 288
PspN4I GGNNCC 4 cut(s) 236, 557, 558, 842
PspPI GGNCC 2 cut(s) 234, 556
PspPPI RGGWCCY 2 cut(s) 234, 556
PstI CTGCAG 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 445, 749
RsaI GTAC 4 cut(s) 107, 368, 403, 582
RsaNI GTAC 4 cut(s) 106, 367, 402, 581
RseI CAYNNNNRTG 2 cut(s) 716, 935
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 543, 1079
Sau3AI GATC 2 cut(s) 1020, 1035
Sau96I GGNCC 2 cut(s) 234, 556
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 2 cut(s) 207, 242
ScrFI CCNGG 2 cut(s) 290, 553
SduI GDGCHC 3 cut(s) 125, 793, 859
SfaNI GCATC 5 cut(s) 28, 606, 620, 1000, 1162
SfcI CTRYAG 1 cut(s) 16
Sfr274I CTCGAG 1 cut(s) 410
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 2 cut(s) 234, 556
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 2 cut(s) 716, 935
SmlI CTYRAG 3 cut(s) 278, 410, 422
SmoI CTYRAG 3 cut(s) 278, 410, 422
Sse9I AATT 4 cut(s) 450, 626, 950, 1146
SspMI CTAG 2 cut(s) 192, 356
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 288, 551
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 2 cut(s) 380, 1129
TaqI TCGA 9 cut(s) 84, 147, 312, 411, 498, 614, 692, 960, 1071
TasI AATT 4 cut(s) 450, 626, 950, 1146
TatI WGTACW 4 cut(s) 105, 366, 401, 580
TauI GCSGC 6 cut(s) 58, 61, 64, 67, 70, 846
TfiI GAWTC 2 cut(s) 149, 307
Tru1I TTAA 2 cut(s) 543, 1079
Tru9I TTAA 2 cut(s) 543, 1079
TscAI CASTG 8 cut(s) 223, 271, 307, 438, 675, 798, 978, 1134
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 6 cut(s) 134, 458, 548, 746, 749, 758
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 8 cut(s) 223, 271, 307, 438, 675, 798, 978, 1134
Van91I CCANNNNNTGG 2 cut(s) 295, 391
VneI GTGCAC 1 cut(s) 789
VpaK11BI GGWCC 2 cut(s) 234, 556
XapI RAATTY 1 cut(s) 950
XhoI CTCGAG 1 cut(s) 410
XspI CTAG 2 cut(s) 192, 356
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.