Rroxscaffold_2G00140350

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002238
Physical Location & Seq
Reverse (-)
78589791 .. 78600106
10316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140350.1

Sequence Viewer

Length: 1362 bp
ATGGCATTAGATATAGTGAGCATTCCAGAGACATTGGATTCGGCAACAAACATGGAGGTGGGAGTCGAACGAACTGAAGGGACAGGGGAAAAGGATAGAGAAATATGGGTAGTCCCGTGTAAAGAAGCAGAGAAGGAAACCTCAACACCTACAGTTCCTGTTTTGGCTGCTCACAAAAGGCAAGCGTCAGTAGAAACACTTCTGAAAAATAACAAAGAAAACTATGCTGCTACACGCGTTCAAGAAGCCCTTAGGAGTACTGAAAAATTAGAGGTGGATTATACCAATATCAAATCAGAGTACACAGCCCATTTGAGCTCTTATGAGGCAGCCTGTAAGCTCGATTCAGGTTTACAATCGTTTGATGTATCTCTCCAACAGCGCACTAGCCGAGTCTTCAACTCCCTTTCCACTGGAGTTGAAGTTAGGTCCCTATCTTTGGACTCACTCAAAGAGGTCACTGATTGTTTGCTTGAGATGAACCAGGAAGTGGTCAGTGTCATTCTCGAATGCAAGGAGGATGTATGGAACAATCAAGAGATGTTCTCTCTAGTGGAGGAGTACTTTGAGAACTGTCTCCAGACATTGGACTTCTGTACTGCCCTCGAGAGATGTCTCAAGCGTGCAGTGAACAATCAGCTGAAAATTCAGGCTGTGATGAGGCGGTTTGAGGAAGAAGTCGAAGCTGGTTTCGAAGGGAAGGGGTGTGTGAGGACATTGCAGGAGTTGAGGGCATTTAAGGCAGCCGGGGACCCTTTCACGGAGGAGTTCTTTGTACTATTTCAGGCAGTTTATAACCAACAGGCATCAATGCTCCAGAAATTGCAAGTCCGAAAGAGAAAGCTTGATAAGAAGTTGAAATCAGTGAAAGCTTGGCGGAAAGTGTCAAATGTTATTTTTGTTGCCACTTTTGTGTCTGTATTGATATTTTCGGTGGTGGCAGCTGCCATTGCTGCATCGCCACTTGTAACCGCCTTGGCAGGTGCACTGGCTGTTCCAATAGGTTCTGTTGGAAAATGGTGTAATATGCTTTGGAGCCTCTTTGAGAGGGCTCTGAAAGGGCAGGGGGAGATAATAAGCTCAATGCAGGTTGGAAGTATGATTACAATGACAGACTTGAACAACATCAGGGTGCTTGTTGAGAAATTTGAAATCGAGATTGAGGCATTGCTGCAGAATGCTGACTTTGCCATTAGAGAAGAGGATGCTGTGAAGATAGTGATGATTGAGATCAACAAAAAGCTGGAAAAGTTTATGGAAACCATCGAAAGTTTAAGCCAAAACGCTGATAAGTGTGGCCGGGATATAAGGAAGGCAAGGACAGTGATTTTGCAGAGAATAATTAGGCATCCCATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

453

Amino Acids

51.05

Weight (kDa)

5.56

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 118 - 447 1.4e-124 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 795
AarI CACCTGC 1 cut(s) 971
Acc36I ACCTGC 2 cut(s) 971, 1078
AccB7I CCANNNNNTGG 2 cut(s) 490, 586
AccII CGCG 1 cut(s) 237
AciI CCGC 3 cut(s) 664, 877, 972
AcoI YGGCCR 1 cut(s) 1297
AcsI RAATTY 2 cut(s) 645, 1145
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 5 cut(s) 259, 302, 563, 598, 777
AfiI CCNNNNNNNGG 4 cut(s) 439, 490, 586, 760
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 6 cut(s) 242, 400, 422, 859, 1120, 1151
AjnI CCWGG 1 cut(s) 483
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 9 cut(s) 318, 340, 640, 686, 844, 872, 944, 1080, 1243
AluI AGCT 9 cut(s) 318, 340, 640, 686, 844, 872, 944, 1080, 1243
Alw21I GWGCWC 2 cut(s) 320, 988
Alw26I GTCTC 3 cut(s) 23, 581, 620
Alw44I GTGCAC 1 cut(s) 984
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 605
AoxI GGCC 1 cut(s) 1297
ApaLI GTGCAC 1 cut(s) 984
ApeKI GCWGC 8 cut(s) 167, 227, 329, 743, 941, 944, 953, 1171
ApoI RAATTY 2 cut(s) 645, 1145
ArsI GACNNNNNNTTYG 4 cut(s) 22, 54, 1312, 1344
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 429, 751
AsuC2I CCSGG 2 cut(s) 748, 1301
AsuII TTCGAA 1 cut(s) 693
AvaI CYCGRG 1 cut(s) 605
AvaII GGWCC 2 cut(s) 429, 751
AxyI CCTNAGG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 988
BanII GRGCYC 2 cut(s) 320, 1054
BarI GAAGNNNNNNTAC 4 cut(s) 183, 215, 1087, 1119
BbsI GAAGAC 1 cut(s) 388
Bbv12I GWGCWC 2 cut(s) 320, 988
BbvI GCAGC 8 cut(s) 154, 214, 341, 755, 931, 940, 953, 1158
BccI CCATC 2 cut(s) 1271, 1361
BciT130I CCWGG 1 cut(s) 485
BcnI CCSGG 2 cut(s) 748, 1301
BcoDI GTCTC 3 cut(s) 23, 581, 620
BfaI CTAG 2 cut(s) 387, 551
BfmI CTRYAG 2 cut(s) 150, 1172
BfuAI ACCTGC 2 cut(s) 971, 1078
BisI GCNGC 8 cut(s) 168, 228, 330, 744, 942, 945, 954, 1172
BlsI GCNGC 8 cut(s) 169, 229, 331, 745, 943, 946, 955, 1173
BmcAI AGTACT 2 cut(s) 259, 563
Bme1390I CCNGG 3 cut(s) 485, 748, 1301
Bme18I GGWCC 2 cut(s) 429, 751
BmeT110I CYCGRG 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 429, 751
BmiI GGNNCC 4 cut(s) 431, 752, 753, 1037
BmrFI CCNGG 3 cut(s) 485, 748, 1301
BmsI GCATC 4 cut(s) 815, 965, 1195, 1357
BpiI GAAGAC 1 cut(s) 388
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 3 cut(s) 435, 563, 800
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 494, 602
BpuMI CCSGG 2 cut(s) 748, 1301
BsaBI GATNNNNATC 1 cut(s) 1229
BsaJI CCNNGG 2 cut(s) 747, 975
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 439, 490, 586, 760
Bse1I ACTGG 2 cut(s) 418, 993
Bse21I CCTNAGG 1 cut(s) 251
Bse3DI GCAATG 3 cut(s) 716, 948, 1166
Bse8I GATNNNNATC 1 cut(s) 1229
BseBI CCWGG 1 cut(s) 485
BseDI CCNNGG 2 cut(s) 747, 975
BseGI GGATG 3 cut(s) 526, 1210, 1348
BseJI GATNNNNATC 1 cut(s) 1229
BseLI CCNNNNNNNGG 4 cut(s) 439, 490, 586, 760
BseMI GCAATG 3 cut(s) 716, 948, 1166
BseNI ACTGG 2 cut(s) 418, 993
BseRI GAGGAG 2 cut(s) 572, 779
BseSI GKGCMC 1 cut(s) 988
BseXI GCAGC 8 cut(s) 154, 214, 341, 755, 931, 940, 953, 1158
BsgI GTGCAG 1 cut(s) 645
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 1 cut(s) 1299
BsiHKAI GWGCWC 2 cut(s) 320, 988
BsiHKCI CYCGRG 1 cut(s) 605
BsiSI CCGG 2 cut(s) 747, 1300
BslFI GGGAC 4 cut(s) 94, 98, 415, 764
BslI CCNNNNNNNGG 4 cut(s) 439, 490, 586, 760
BsmAI GTCTC 3 cut(s) 23, 581, 620
BsmFI GGGAC 4 cut(s) 94, 98, 415, 764
BsmI GAATGC 3 cut(s) 21, 515, 1183
BsnI GGCC 1 cut(s) 1299
BsoBI CYCGRG 1 cut(s) 605
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 3 cut(s) 320, 988, 1054
Bsp143I GATC 1 cut(s) 1230
BspACI CCGC 3 cut(s) 664, 877, 972
BspANI GGCC 1 cut(s) 1299
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 4 cut(s) 431, 752, 753, 1037
BspMAI CTGCAG 1 cut(s) 1176
BspMI ACCTGC 2 cut(s) 971, 1078
BspT104I TTCGAA 1 cut(s) 693
BsrDI GCAATG 3 cut(s) 716, 948, 1166
BsrI ACTGG 2 cut(s) 418, 993
BssECI CCNNGG 2 cut(s) 747, 975
BssMI GATC 1 cut(s) 1230
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 485
Bst4CI ACNGT 3 cut(s) 154, 575, 1324
Bst6I CTCTTC 1 cut(s) 1194
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 183, 624
BstDEI CTNAG 1 cut(s) 251
BstF5I GGATG 3 cut(s) 526, 1210, 1348
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 1 cut(s) 1233
BstMAI GTCTC 3 cut(s) 23, 581, 620
BstMBI GATC 1 cut(s) 1230
BstMWI GCNNNNNNNGC 4 cut(s) 740, 950, 953, 1187
BstNI CCWGG 1 cut(s) 485
BstSCI CCNGG 3 cut(s) 483, 746, 1299
BstSFI CTRYAG 2 cut(s) 150, 1172
BstSLI GKGCMC 1 cut(s) 988
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 8 cut(s) 154, 214, 341, 755, 931, 940, 953, 1158
BstV2I GAAGAC 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 251
BsuRI GGCC 1 cut(s) 1299
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 3 cut(s) 526, 1210, 1348
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 7 cut(s) 411, 459, 502, 633, 870, 986, 1329
BveI ACCTGC 2 cut(s) 971, 1078
Cac8I GCNNGC 2 cut(s) 183, 624
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 429, 751
CseI GACGC 1 cut(s) 174
Csp6I GTAC 5 cut(s) 258, 301, 562, 597, 776
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 1 cut(s) 52
CviQI GTAC 5 cut(s) 258, 301, 562, 597, 776
DdeI CTNAG 1 cut(s) 251
DpnI GATC 1 cut(s) 1232
DpnII GATC 1 cut(s) 1230
EaeI YGGCCR 1 cut(s) 1297
Eam1104I CTCTTC 1 cut(s) 1194
EarI CTCTTC 1 cut(s) 1194
EciI GGCGGA 1 cut(s) 892
Ecl136II GAGCTC 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 975
Eco24I GRGCYC 2 cut(s) 320, 1054
Eco47I GGWCC 2 cut(s) 429, 751
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 251
Eco88I CYCGRG 1 cut(s) 605
EcoICRI GAGCTC 1 cut(s) 318
EcoO109I RGGNCCY 2 cut(s) 429, 751
EcoRII CCWGG 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 975
EcoT38I GRGCYC 2 cut(s) 320, 1054
ErhI CCWWGG 1 cut(s) 975
FaeI CATG 1 cut(s) 55
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 4 cut(s) 94, 98, 415, 764
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 8 cut(s) 168, 228, 330, 744, 942, 945, 954, 1172
FokI GGATG 3 cut(s) 533, 1217, 1335
FriOI GRGCYC 2 cut(s) 320, 1054
Fsp4HI GCNGC 8 cut(s) 168, 228, 330, 744, 942, 945, 954, 1172
FspBI CTAG 2 cut(s) 387, 551
GlaI GCGC 1 cut(s) 383
GluI GCNGC 8 cut(s) 168, 228, 330, 744, 942, 945, 954, 1172
GsuI CTGGAG 3 cut(s) 435, 563, 800
HaeIII GGCC 1 cut(s) 1299
HapII CCGG 2 cut(s) 747, 1300
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 384
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 842, 870
HinfI GANTC 5 cut(s) 38, 63, 344, 393, 443
HpaII CCGG 2 cut(s) 747, 1300
Hpy166II GTNNAC 4 cut(s) 303, 353, 631, 986
Hpy188I TCNGA 4 cut(s) 204, 298, 833, 1056
Hpy188III TCNNGA 8 cut(s) 26, 242, 506, 536, 580, 607, 817, 1156
Hpy8I GTNNAC 4 cut(s) 303, 353, 631, 986
HpyAV CCTTC 5 cut(s) 71, 127, 689, 694, 1306
HpyCH4III ACNGT 3 cut(s) 154, 575, 1324
HpyCH4V TGCA 9 cut(s) 513, 626, 721, 826, 956, 986, 1087, 1174, 1333
HpyF10VI GCNNNNNNNGC 4 cut(s) 740, 950, 953, 1187
HpyF3I CTNAG 1 cut(s) 251
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 382
KflI GGGWCCC 1 cut(s) 751
Kzo9I GATC 1 cut(s) 1230
LmnI GCTCC 2 cut(s) 819, 1035
Lsp1109I GCAGC 8 cut(s) 154, 214, 341, 755, 931, 940, 953, 1158
LweI GCATC 4 cut(s) 815, 965, 1195, 1357
MaeI CTAG 2 cut(s) 387, 551
MaeIII GTNAC 2 cut(s) 457, 967
MalI GATC 1 cut(s) 1232
MboI GATC 1 cut(s) 1230
MboII GAAGA 4 cut(s) 388, 686, 1211, 1225
MhlI GDGCHC 3 cut(s) 320, 988, 1054
MluCI AATT 5 cut(s) 266, 645, 821, 1145, 1341
MluI ACGCGT 1 cut(s) 235
MlyI GAGTC 3 cut(s) 72, 402, 437
MmeI TCCRAC 3 cut(s) 400, 991, 1072
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 2 cut(s) 738, 1274
MslI CAYNNNNRTG 3 cut(s) 56, 911, 1130
MspA1I CMGCKG 2 cut(s) 640, 944
MspI CCGG 2 cut(s) 747, 1300
MspR9I CCNGG 3 cut(s) 485, 748, 1301
Mva1269I GAATGC 3 cut(s) 21, 515, 1183
MvaI CCWGG 1 cut(s) 485
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 4 cut(s) 740, 950, 953, 1187
NciI CCSGG 2 cut(s) 748, 1301
NdeII GATC 1 cut(s) 1230
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 4 cut(s) 431, 752, 753, 1037
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 1 cut(s) 457
NspV TTCGAA 1 cut(s) 693
OliI CACNNNNGTG 1 cut(s) 911
PaeR7I CTCGAG 1 cut(s) 605
PaqCI CACCTGC 1 cut(s) 971
PctI GAATGC 3 cut(s) 21, 515, 1183
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 38, 344
PflMI CCANNNNNTGG 2 cut(s) 490, 586
PkrI GCNGC 8 cut(s) 169, 229, 331, 745, 943, 946, 955, 1173
PleI GAGTC 3 cut(s) 71, 401, 437
PpsI GAGTC 3 cut(s) 71, 401, 437
PpuMI RGGWCCY 2 cut(s) 429, 751
PsiI TTATAA 1 cut(s) 795
Psp124BI GAGCTC 1 cut(s) 320
Psp5II RGGWCCY 2 cut(s) 429, 751
Psp6I CCWGG 1 cut(s) 483
PspGI CCWGG 1 cut(s) 483
PspN4I GGNNCC 4 cut(s) 431, 752, 753, 1037
PspPI GGNCC 2 cut(s) 429, 751
PspPPI RGGWCCY 2 cut(s) 429, 751
PstI CTGCAG 1 cut(s) 1176
PstNI CAGNNNCTG 1 cut(s) 158
PvuII CAGCTG 2 cut(s) 640, 944
RsaI GTAC 5 cut(s) 259, 302, 563, 598, 777
RsaNI GTAC 5 cut(s) 258, 301, 562, 597, 776
RseI CAYNNNNRTG 3 cut(s) 56, 911, 1130
SacI GAGCTC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 738, 1274
SatI GCNGC 8 cut(s) 168, 228, 330, 744, 942, 945, 954, 1172
Sau3AI GATC 1 cut(s) 1230
Sau96I GGNCC 2 cut(s) 429, 751
ScaI AGTACT 2 cut(s) 259, 563
SchI GAGTC 3 cut(s) 72, 402, 437
ScrFI CCNGG 3 cut(s) 485, 748, 1301
SduI GDGCHC 3 cut(s) 320, 988, 1054
SfaNI GCATC 4 cut(s) 815, 965, 1195, 1357
SfcI CTRYAG 2 cut(s) 150, 1172
Sfr274I CTCGAG 1 cut(s) 605
SfuI TTCGAA 1 cut(s) 693
SinI GGWCC 2 cut(s) 429, 751
SlaI CTCGAG 1 cut(s) 605
SmiMI CAYNNNNRTG 3 cut(s) 56, 911, 1130
SmlI CTYRAG 3 cut(s) 473, 605, 617
SmoI CTYRAG 3 cut(s) 473, 605, 617
Sse9I AATT 5 cut(s) 266, 645, 821, 1145, 1341
SsiI CCGC 3 cut(s) 664, 877, 972
SspMI CTAG 2 cut(s) 387, 551
SstI GAGCTC 1 cut(s) 320
StyD4I CCNGG 3 cut(s) 483, 746, 1299
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 3 cut(s) 154, 575, 1324
TaqI TCGA 8 cut(s) 66, 342, 507, 606, 681, 693, 1155, 1266
TasI AATT 5 cut(s) 266, 645, 821, 1145, 1341
TatI WGTACW 5 cut(s) 257, 300, 561, 596, 775
TfiI GAWTC 2 cut(s) 38, 344
Tru1I TTAA 2 cut(s) 738, 1274
Tru9I TTAA 2 cut(s) 738, 1274
TscAI CASTG 7 cut(s) 418, 466, 502, 633, 870, 993, 1329
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 8 cut(s) 167, 227, 329, 743, 941, 944, 953, 1171
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 7 cut(s) 418, 466, 502, 633, 870, 993, 1329
Van91I CCANNNNNTGG 2 cut(s) 490, 586
VneI GTGCAC 1 cut(s) 984
VpaK11BI GGWCC 2 cut(s) 429, 751
XapI RAATTY 2 cut(s) 645, 1145
XhoI CTCGAG 1 cut(s) 605
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 387, 551
ZrmI AGTACT 2 cut(s) 259, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.