pycom02g15720

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002238
Physical Location & Seq
Forward (+)
12681320 .. 12682251
932 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15720.1

Sequence Viewer

Length: 708 bp
ATGGAGGTGGTAATAGGTGTGCGTACACCATCGATAGGCTGTCGTTGGGCAAGGGAGCATGATGGTCGAGCTCAGTTGGATTCCAGCCTCCTTGCGATGAGTTTTGTAGCAATGATCTTGAAGTTTGTGAAGTTTGATTTTCTCGAATTAACAGTTTGGAATTGTGCAATAGGCAAATGTAAGAGAACCTTAAAAGAATTGAGGGCATTTAAGGTAGCTGGGGATCATTTCATGGATGAGTTCTTTGTACTGTTTCAGGCTGTTAATAAGAACCGGGCATCGATGTTTGAGAAACTGCAACTTCGTAAAAGAAAACTTGATAAGAAGTTGAAATCAGTGAATATTGGTTTCTCTGTAGTGTCAGCTGCCATTGCTGCACCACTACTTGTAAAGGCCTTAGCAGGAGCATTCATTGTTCCAATAGGTTTAGTTGGAAAATGGTGTAATATACTTTGGAATAACTACGAGAGGGGATTGAAAGGGAAGGACTTGGACAACATTCGGGTGCTTGCTGACAAATTTGAAATTGGTATTGAATCACTGTTGCACAATGCTGGTTTTGCTATTAAAGAAGAGAGCGTAGCTATGAAGCTAGTGATAGATGAAATCAAGAAGAAGCTTGATGTGTTTGTGGAAACCATTGATAATGTGAGCCAACATGCTGATAATTCTAACCAGAATATAAGGGCAGTGATTCTGAGAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

236

Amino Acids

26.49

Weight (kDa)

9.43

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 60 - 229 9.8e-28 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 231
AcsI RAATTY 1 cut(s) 518
AfaI GTAC 2 cut(s) 25, 249
AfiI CCNNNNNNNGG 1 cut(s) 35
AgsI TTSAA 5 cut(s) 121, 331, 478, 524, 536
AluBI AGCT 6 cut(s) 71, 218, 365, 584, 592, 619
AluI AGCT 6 cut(s) 71, 218, 365, 584, 592, 619
Alw21I GWGCWC 1 cut(s) 73
AlwI GGATC 1 cut(s) 231
AoxI GGCC 1 cut(s) 393
ApeKI GCWGC 2 cut(s) 365, 374
ApoI RAATTY 1 cut(s) 518
AsuC2I CCSGG 1 cut(s) 275
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 352, 361
BccI CCATC 2 cut(s) 37, 56
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BcnI CCSGG 1 cut(s) 275
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 354
BisI GCNGC 2 cut(s) 366, 375
BlsI GCNGC 2 cut(s) 367, 376
Bme1390I CCNGG 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 2 cut(s) 32, 281
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 117, 369
BseCI ATCGAT 2 cut(s) 32, 281
BseGI GGATG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMI GCAATG 2 cut(s) 117, 369
BseMII CTCAG 2 cut(s) 86, 689
BseXI GCAGC 2 cut(s) 352, 361
BseYI CCCAGC 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 2 cut(s) 32, 281
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 35
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 114, 223
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 85, 690
BspDI ATCGAT 2 cut(s) 32, 281
BspPI GGATC 1 cut(s) 231
BsrDI GCAATG 2 cut(s) 117, 369
BssMI GATC 2 cut(s) 114, 223
Bst4CI ACNGT 3 cut(s) 154, 252, 543
Bst6I CTCTTC 1 cut(s) 567
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 3 cut(s) 72, 397, 698
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 2 cut(s) 117, 226
BstMBI GATC 2 cut(s) 114, 223
BstMWI GCNNNNNNNGC 3 cut(s) 371, 374, 560
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 1 cut(s) 273
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 2 cut(s) 352, 361
Bsu15I ATCGAT 2 cut(s) 32, 281
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 2 cut(s) 32, 281
BtgZI GCGATG 1 cut(s) 110
BtsCI GGATG 1 cut(s) 241
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 342, 539, 696
Cac8I GCNNGC 1 cut(s) 510
ClaI ATCGAT 2 cut(s) 32, 281
Csp6I GTAC 2 cut(s) 24, 248
CviAII CATG 3 cut(s) 59, 232, 659
CviQI GTAC 2 cut(s) 24, 248
DdeI CTNAG 3 cut(s) 72, 397, 698
DpnI GATC 2 cut(s) 116, 225
DpnII GATC 2 cut(s) 114, 223
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 71
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 73
Eco53kI GAGCTC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 71
EcoT38I GRGCYC 1 cut(s) 73
FaeI CATG 3 cut(s) 62, 235, 662
FaiI YATR 6 cut(s) 60, 233, 449, 587, 660, 683
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FatI CATG 3 cut(s) 58, 231, 658
Fnu4HI GCNGC 2 cut(s) 366, 375
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 2 cut(s) 366, 375
FspBI CTAG 1 cut(s) 593
GluI GCNGC 2 cut(s) 366, 375
GsaI CCCAGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 274
Hin1II CATG 3 cut(s) 62, 235, 662
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 3 cut(s) 80, 536, 694
HpaII CCGG 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 26
Hpy188I TCNGA 1 cut(s) 699
Hpy188III TCNNGA 3 cut(s) 118, 143, 610
Hpy8I GTNNAC 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 478
HpyCH4III ACNGT 3 cut(s) 154, 252, 543
HpyCH4V TGCA 4 cut(s) 167, 298, 377, 547
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 374, 560
HpyF3I CTNAG 3 cut(s) 72, 397, 698
Hsp92II CATG 3 cut(s) 62, 235, 662
Kzo9I GATC 2 cut(s) 114, 223
LmnI GCTCC 2 cut(s) 55, 404
LpnPI CCDG 7 cut(s) 97, 204, 242, 287, 387, 540, 689
Lsp1109I GCAGC 2 cut(s) 352, 361
LweI GCATC 1 cut(s) 287
MaeI CTAG 1 cut(s) 593
MalI GATC 2 cut(s) 116, 225
MboI GATC 2 cut(s) 114, 223
MboII GAAGA 2 cut(s) 584, 625
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 6 cut(s) 146, 160, 197, 518, 525, 667
MmeI TCCRAC 2 cut(s) 57, 412
MnlI CCTC 3 cut(s) 98, 195, 462
MseI TTAA 5 cut(s) 149, 191, 210, 264, 567
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 274
MspR9I CCNGG 1 cut(s) 275
Mva1269I GAATGC 1 cut(s) 407
MwoI GCNNNNNNNGC 3 cut(s) 371, 374, 560
NciI CCSGG 1 cut(s) 275
NdeII GATC 2 cut(s) 114, 223
NlaIII CATG 3 cut(s) 62, 235, 662
NspI RCATGY 1 cut(s) 662
PceI AGGCCT 1 cut(s) 395
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 3 cut(s) 80, 536, 694
PkrI GCNGC 2 cut(s) 367, 376
Psp124BI GAGCTC 1 cut(s) 73
PspFI CCCAGC 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 25, 249
RsaNI GTAC 2 cut(s) 24, 248
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 5 cut(s) 149, 191, 210, 264, 567
SatI GCNGC 2 cut(s) 366, 375
Sau3AI GATC 2 cut(s) 114, 223
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 1 cut(s) 503
Sse9I AATT 6 cut(s) 146, 160, 197, 518, 525, 667
SseBI AGGCCT 1 cut(s) 395
SspI AATATT 1 cut(s) 343
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 73
StuI AGGCCT 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 3 cut(s) 154, 252, 543
TaqI TCGA 4 cut(s) 32, 67, 144, 281
TasI AATT 6 cut(s) 146, 160, 197, 518, 525, 667
TatI WGTACW 1 cut(s) 247
TfiI GAWTC 3 cut(s) 80, 536, 694
Tru1I TTAA 5 cut(s) 149, 191, 210, 264, 567
Tru9I TTAA 5 cut(s) 149, 191, 210, 264, 567
TscAI CASTG 3 cut(s) 342, 546, 696
TseI GCWGC 2 cut(s) 365, 374
TspDTI ATGAA 4 cut(s) 220, 400, 602, 618
TspRI CASTG 3 cut(s) 342, 546, 696
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 662
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.